MD12G1249700.v1.1

Caleosin related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011702
Physical Location & Seq
Forward (+)
31904823 .. 31906555
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1249700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGCAGCTGATCAGGGCCAGCAGCAGGCATCCAGTGAGTCCATGGCGACGGTGGCTGAGAAGGCTCCGATCACCGTGGAGAGGAAGATCCGCAATGATTTGGAGACCAAGCTCCCCAAACCATACATGCCTAGGGCTATGATTGCGCCTGATATGGGGAACATCAATGGCACGTGGGGCCATAAGCATTCCAACATGTCTGTGCTTCAACAGCATGCAGCATTCTTCGACCAAGACAACGATGGTATAATCTACCCTTCCGAAACATTCAGAGGATTTCGTGCACTCGGGTTTAATCCGGTTGCTTCGTTCATCTTCATGGTTCTTGTCCATGGAGCTATGAGTTATGCTACTCTTCCTACATGGATACCGTCGCCTTTCTTCGCAATTCACATAGAGAACATACACAGGGCAAAACATGGAAGTGACTCAGGGACCTATGACACAGAGGGAAGGTACATTCCTGCAAATCTGGAGAACATGTTCAGCAAGTACGCCCGCACTGTGCCTGATAAGCTTACATTCAAGGAGCTTTGGCACATGACTCAGGCTAACCGTGATGCCTTTGATTTCTTTGGCTGGATTGCAAGTAAACTGGAATGGGGAGTTCTGTATCTTCTTGCAAAAGATGAAAACGGCTACTTGGCAAAGGAAGCTGTGAGGCGTTGTTTTGATGGAAGCTTGTTCGAGTACTGTTCGAAGTTGCAGAAGGGTGCTGTTAGTAAGATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
Caleosin

Protein Analysis

245

Amino Acids

27.53

Weight (kDa)

7.1

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Caleosin PF05042 65 - 232 1.5e-78 Caleosin related protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 91, 499
AclWI GGATC 1 cut(s) 82
AcvI CACGTG 1 cut(s) 174
AfaI GTAC 3 cut(s) 458, 494, 692
AfiI CCNNNNNNNGG 3 cut(s) 25, 81, 155
AflIII ACRYGT 2 cut(s) 195, 480
AgsI TTSAA 2 cut(s) 209, 526
AloI GAACNNNNNNTCC 2 cut(s) 467, 499
AluBI AGCT 7 cut(s) 8, 112, 338, 517, 532, 656, 681
AluI AGCT 7 cut(s) 8, 112, 338, 517, 532, 656, 681
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 1 cut(s) 98
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 16, 178
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 5, 22, 218
Asp700I GAANNNNTTC 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 16, 178, 435
AsuHPI GGTGA 1 cut(s) 64
AsuII TTCGAA 1 cut(s) 698
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 435
AvrII CCTAGG 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 286
BbrPI CACGTG 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 3 cut(s) 17, 34, 230
BccI CCATC 3 cut(s) 236, 668, 721
BceAI ACGGC 1 cut(s) 652
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 1 cut(s) 132
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 3 cut(s) 6, 23, 219
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 3 cut(s) 7, 24, 220
BmcAI AGTACT 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 16, 178, 435
BmiI GGNNCC 3 cut(s) 66, 179, 436
BmsI GCATC 2 cut(s) 38, 550
BpmI CTGGAG 1 cut(s) 494
Bpu14I TTCGAA 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 4 cut(s) 42, 75, 131, 331
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 81, 155
Bse1I ACTGG 2 cut(s) 33, 600
Bse3DI GCAATG 1 cut(s) 100
BseDI CCNNGG 4 cut(s) 42, 75, 131, 331
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 3 cut(s) 25, 81, 155
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 48, 444, 560
BseNI ACTGG 2 cut(s) 33, 600
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 3 cut(s) 17, 34, 230
BshFI GGCC 2 cut(s) 18, 180
BsiHKAI GWGCWC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 3 cut(s) 25, 81, 155
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 187, 221
BsnI GGCC 2 cut(s) 18, 180
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 10, 69, 87
Bsp19I CCATGG 2 cut(s) 42, 331
BspACI CCGC 2 cut(s) 91, 499
BspANI GGCC 2 cut(s) 18, 180
BspCNI CTCAG 3 cut(s) 49, 443, 559
BspLI GGNNCC 3 cut(s) 66, 179, 436
BspPI GGATC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 2 cut(s) 33, 600
BssECI CCNNGG 4 cut(s) 42, 75, 131, 331
BssMI GATC 3 cut(s) 10, 69, 87
BssT1I CCWWGG 3 cut(s) 42, 131, 331
Bst4CI ACNGT 6 cut(s) 52, 76, 372, 505, 557, 695
Bst6I CTCTTC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 698
BstC8I GCNNGC 4 cut(s) 20, 27, 216, 499
BstDEI CTNAG 3 cut(s) 57, 430, 546
BstDSI CCRYGG 3 cut(s) 42, 75, 331
BstF5I GGATG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 3 cut(s) 13, 72, 90
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 10, 69, 87
BstMWI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
BstNSI RCATGY 4 cut(s) 130, 199, 218, 484
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 3 cut(s) 17, 34, 230
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 18, 180
BtgI CCRYGG 3 cut(s) 42, 75, 331
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 40, 501
Cac8I GCNNGC 4 cut(s) 20, 27, 216, 499
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 16, 178, 435
Csp6I GTAC 3 cut(s) 457, 493, 691
CviQI GTAC 3 cut(s) 457, 493, 691
DdeI CTNAG 3 cut(s) 57, 430, 546
DpnI GATC 3 cut(s) 12, 71, 89
DpnII GATC 3 cut(s) 10, 69, 87
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco130I CCWWGG 3 cut(s) 42, 131, 331
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 435
Eco72I CACGTG 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 435
EcoT14I CCWWGG 3 cut(s) 42, 131, 331
ErhI CCWWGG 3 cut(s) 42, 131, 331
FaqI GGGAC 1 cut(s) 448
FauI CCCGC 1 cut(s) 506
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 3 cut(s) 6, 23, 219
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 3 cut(s) 6, 23, 219
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 147
GluI GCNGC 3 cut(s) 6, 23, 219
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 2 cut(s) 18, 180
HapII CCGG 1 cut(s) 299
HhaI GCGC 1 cut(s) 148
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HindIII AAGCTT 2 cut(s) 515, 679
HinfI GANTC 3 cut(s) 38, 428, 544
HpaII CCGG 1 cut(s) 299
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 284, 593
Hpy188I TCNGA 3 cut(s) 69, 262, 272
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 2 cut(s) 284, 593
Hpy99I CGWCG 2 cut(s) 52, 376
HpyAV CCTTC 4 cut(s) 55, 267, 447, 703
HpyCH4III ACNGT 6 cut(s) 52, 76, 372, 505, 557, 695
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 6 cut(s) 218, 284, 467, 587, 623, 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
HpyF3I CTNAG 3 cut(s) 57, 430, 546
HpySE526I ACGT 1 cut(s) 173
HspAI GCGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 3 cut(s) 10, 69, 87
LmnI GCTCC 4 cut(s) 70, 117, 335, 529
Lsp1109I GCAGC 3 cut(s) 17, 34, 230
LweI GCATC 2 cut(s) 38, 550
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 1 cut(s) 425
MalI GATC 3 cut(s) 12, 71, 89
MboI GATC 3 cut(s) 10, 69, 87
MboII GAAGA 6 cut(s) 97, 217, 307, 347, 373, 608
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 1 cut(s) 387
MlyI GAGTC 3 cut(s) 47, 422, 538
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 4 cut(s) 75, 266, 442, 654
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 3 cut(s) 200, 317, 423
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 299
Mva1269I GAATGC 2 cut(s) 187, 221
MwoI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
NcoI CCATGG 2 cut(s) 42, 331
NdeII GATC 3 cut(s) 10, 69, 87
NlaIV GGNNCC 3 cut(s) 66, 179, 436
NmuCI GTSAC 1 cut(s) 425
NspI RCATGY 4 cut(s) 130, 199, 218, 484
NspV TTCGAA 1 cut(s) 698
PaeI GCATGC 1 cut(s) 218
PciI ACATGT 2 cut(s) 195, 480
PctI GAATGC 2 cut(s) 187, 221
PdmI GAANNNNTTC 1 cut(s) 482
PkrI GCNGC 3 cut(s) 7, 24, 220
PleI GAGTC 3 cut(s) 46, 422, 538
PmaCI CACGTG 1 cut(s) 174
PmlI CACGTG 1 cut(s) 174
PpsI GAGTC 3 cut(s) 46, 422, 538
Ppu21I YACGTR 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 435
PscI ACATGT 2 cut(s) 195, 480
Psp5II RGGWCCY 1 cut(s) 435
PspCI CACGTG 1 cut(s) 174
PspN4I GGNNCC 3 cut(s) 66, 179, 436
PspPI GGNCC 3 cut(s) 16, 178, 435
PspPPI RGGWCCY 1 cut(s) 435
PsuI RGATCY 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 458, 494, 692
RsaNI GTAC 3 cut(s) 457, 493, 691
RseI CAYNNNNRTG 3 cut(s) 200, 317, 423
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 6, 23, 219
Sau3AI GATC 3 cut(s) 10, 69, 87
Sau96I GGNCC 3 cut(s) 16, 178, 435
ScaI AGTACT 1 cut(s) 692
SchI GAGTC 3 cut(s) 47, 422, 538
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 2 cut(s) 38, 550
SfuI TTCGAA 1 cut(s) 698
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 3 cut(s) 200, 317, 423
SphI GCATGC 1 cut(s) 218
Sse9I AATT 1 cut(s) 387
SsiI CCGC 2 cut(s) 91, 499
SspMI CTAG 1 cut(s) 132
StyI CCWWGG 3 cut(s) 42, 131, 331
TaaI ACNGT 6 cut(s) 52, 76, 372, 505, 557, 695
TaiI ACGT 1 cut(s) 176
TaqI TCGA 3 cut(s) 228, 687, 698
TasI AATT 1 cut(s) 387
TatI WGTACW 1 cut(s) 690
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 40, 508
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 3 cut(s) 5, 22, 218
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 3 cut(s) 301, 307, 645
TspRI CASTG 2 cut(s) 40, 508
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 1 cut(s) 435
XceI RCATGY 4 cut(s) 130, 199, 218, 484
XcmI CCANNNNNNNNNTGG 3 cut(s) 40, 49, 239
XmaJI CCTAGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 132
ZrmI AGTACT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.