MD04G1243900.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012632
Physical Location & Seq
Forward (+)
32032868 .. 32036596
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1243900.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGAAGTCCTCCTCTGTTACTTTTCGTGCAGATTCGAGAAGCGGAAAATCATGTTTTTGTAGTATTATCCCGGCAGCTTGTCTGCTGTGTATATTATTTCTCATAGTGAGTGTATACATATCATCATCAGATTACAAAGAGGATTCATCGTCATTTTGGAAAGTCACAAACAATTTGCAAACTCTAAAATACAAATGCAAGAATCAAAATCGGCCTTACGGAAGTGAGCCACTTCCTCGAGGTATTGTTGCCAATACTTCTAATCTGGAAATGCAGCCGTTGTGGCATGCTAAAAAGAAACCTTCGTTTAAATTGTTGGAGAAGAAAGATGCAAATTCTTCATCTAGTTTGTTTGCCATGGCCGTTGGGATAAAGCAAAAAGATCTTGTTTGTAAAATGGTCAAAAAGTTTCTGTCAAGTGGTTTCGTTGTGATGCTCTTCCATTATGATGGCATTGTCGATCAGTGGAAGGAATTTCAATGGAGTGACCAAGTCATTCATGTATCTGCAGTCAATCAAACTAAATGGTGGTTTGCGAAGCGCTTCTTGCATCCTGACATAGTTGCCGAATATAGTTACATTTTCCTCTGGGATGAGGACCTCGGAGTTGACAATTTCCACCCCCAACGGTACGTATCCATTGTTCAAAAGGAAGGGCTTGAGATATCACAACCTGCATTGGATTATTCCAAATCAGAGGTGCATCATCAAATTACTGTGCGTATGAGAGGATCGGTAGCACACAGAAGGACTTACAAGTCAAGCAACAATGGGAAAGGTTGTGATGAAAGCAGTACAGCTCCTCCTTGCACCGGGTGGATAGAAGTGATGGCTCCTGTTTTCTCTCGGGCTGCCTGGCGCTGCGTATGGTATATGATCCAGAATGACTTGATTCATGCTTGGGGACTAGATACACAGATTGGCTATTGTGCACAGGGAGATCGAACTAAAAAAGTTGGGGTTGTGGATGCCGAGTACATAATCCATTACGGCCGCCCTACACTGGGAGGACCTTCAGATGATCAAAGTGATCACGTGACGAAAGATAAAATTCGTTGGCAGGAATCGTCACCATCATCAAAATCTACAAGAAAGGGCCACAGAGTTGATGTGAGGCGGGAATCGTACAACGAGATGAAAGTGTTCAGAAGGAAATGGGAACGAGCCATCAAGAATGATAAATGTTGGATCGATCCCTACGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

402

Amino Acids

46.09

Weight (kDa)

9.21

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 70 - 391 2.7e-138 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 757
AbsI CCTCGAGG 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 682
AccI GTMKAC 1 cut(s) 114
AciI CCGC 3 cut(s) 42, 994, 1117
AclWI GGATC 4 cut(s) 739, 871, 1187, 1196
AcoI YGGCCR 2 cut(s) 360, 991
AcsI RAATTY 3 cut(s) 334, 473, 1050
AcuI CTGAAG 1 cut(s) 999
AcvI CACGTG 1 cut(s) 1036
AdeI CACNNNGTG 1 cut(s) 816
AfaI GTAC 4 cut(s) 632, 796, 977, 1127
AfeI AGCGCT 1 cut(s) 542
AfiI CCNNNNNNNGG 3 cut(s) 812, 1003, 1004
AgsI TTSAA 2 cut(s) 479, 647
AjnI CCWGG 1 cut(s) 854
AluBI AGCT 2 cut(s) 77, 800
AluI AGCT 2 cut(s) 77, 800
Alw21I GWGCWC 1 cut(s) 934
Alw44I GTGCAC 1 cut(s) 930
AlwI GGATC 4 cut(s) 739, 871, 1187, 1196
Ama87I CYCGRG 2 cut(s) 237, 846
Aor51HI AGCGCT 1 cut(s) 542
AoxI GGCC 4 cut(s) 212, 360, 991, 1096
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 4 cut(s) 74, 274, 851, 861
ApoI RAATTY 3 cut(s) 334, 473, 1050
Asp700I GAANNNNTTC 2 cut(s) 542, 1142
AspLEI GCGC 2 cut(s) 543, 861
AspS9I GGNCC 3 cut(s) 598, 1010, 1096
AsuC2I CCSGG 2 cut(s) 71, 814
AsuHPI GGTGA 1 cut(s) 1062
AvaI CYCGRG 2 cut(s) 237, 846
AvaII GGWCC 2 cut(s) 598, 1010
BaeGI GKGCMC 1 cut(s) 934
BbrPI CACGTG 1 cut(s) 1036
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 4 cut(s) 86, 286, 838, 848
BccI CCATC 4 cut(s) 443, 823, 1081, 1175
BceAI ACGGC 3 cut(s) 262, 347, 1006
BciT130I CCWGG 1 cut(s) 856
BciVI GTATCC 1 cut(s) 646
BclI TGATCA 2 cut(s) 1021, 1030
BcnI CCSGG 2 cut(s) 71, 814
BfaI CTAG 2 cut(s) 345, 908
BfmI CTRYAG 1 cut(s) 507
BfoI RGCGCY 2 cut(s) 544, 862
BfuAI ACCTGC 1 cut(s) 682
BfuI GTATCC 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 283
BglII AGATCT 1 cut(s) 382
BisI GCNGC 5 cut(s) 75, 275, 852, 862, 994
BlsI GCNGC 5 cut(s) 76, 276, 853, 863, 995
Bme1390I CCNGG 3 cut(s) 71, 814, 856
Bme18I GGWCC 2 cut(s) 598, 1010
BmeT110I CYCGRG 2 cut(s) 237, 846
BmgT120I GGNCC 3 cut(s) 598, 1010, 1096
BmiI GGNNCC 1 cut(s) 834
BmrFI CCNGG 3 cut(s) 71, 814, 856
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 5 cut(s) 319, 423, 559, 712, 958
BmuI ACTGGG 1 cut(s) 1013
BpuEI CTTGAG 1 cut(s) 680
BpuMI CCSGG 2 cut(s) 71, 814
Bsa29I ATCGAT 1 cut(s) 1191
BsaAI YACGTR 2 cut(s) 634, 1036
BsaJI CCNNGG 2 cut(s) 357, 601
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 3 cut(s) 812, 1003, 1004
Bse1I ACTGG 1 cut(s) 1008
BseBI CCWGG 1 cut(s) 856
BseCI ATCGAT 1 cut(s) 1191
BseDI CCNNGG 2 cut(s) 357, 601
BseGI GGATG 3 cut(s) 550, 598, 973
BseLI CCNNNNNNNGG 3 cut(s) 812, 1003, 1004
BseNI ACTGG 1 cut(s) 1008
BseRI GAGGAG 1 cut(s) 792
BseSI GKGCMC 1 cut(s) 934
BseX3I CGGCCG 1 cut(s) 991
BseXI GCAGC 4 cut(s) 86, 286, 838, 848
BsgI GTGCAG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 994
BshFI GGCC 4 cut(s) 214, 362, 993, 1098
BshVI ATCGAT 1 cut(s) 1191
BsiEI CGRYCG 1 cut(s) 994
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 2 cut(s) 237, 846
BsiSI CCGG 2 cut(s) 71, 813
BslFI GGGAC 1 cut(s) 918
BslI CCNNNNNNNGG 3 cut(s) 812, 1003, 1004
BsmFI GGGAC 1 cut(s) 918
BsnI GGCC 4 cut(s) 214, 362, 993, 1098
BsoBI CYCGRG 2 cut(s) 237, 846
Bsp1286I GDGCHC 1 cut(s) 934
Bsp143I GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 3 cut(s) 42, 994, 1117
BspANI GGCC 4 cut(s) 214, 362, 993, 1098
BspDI ATCGAT 1 cut(s) 1191
BspLI GGNNCC 1 cut(s) 834
BspMAI CTGCAG 1 cut(s) 511
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 4 cut(s) 739, 871, 1187, 1196
BspQI GCTCTTC 1 cut(s) 443
BsrI ACTGG 1 cut(s) 1008
BssECI CCNNGG 2 cut(s) 357, 601
BssMI GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
BssNAI GTATAC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 357
Bst1107I GTATAC 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 856
Bst4CI ACNGT 2 cut(s) 630, 718
Bst6I CTCTTC 1 cut(s) 443
BstBAI YACGTR 2 cut(s) 634, 1036
BstC8I GCNNGC 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 550, 598, 973
BstH2I RGCGCY 2 cut(s) 544, 862
BstHHI GCGC 2 cut(s) 543, 861
BstKTI GATC 9 cut(s) 385, 463, 734, 879, 943, 1024, 1033, 1191, 1195
BstMBI GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
BstMCI CGRYCG 1 cut(s) 994
BstMWI GCNNNNNNNGC 2 cut(s) 283, 547
BstNI CCWGG 1 cut(s) 856
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 69, 812, 854
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 934
BstSNI TACGTA 1 cut(s) 634
BstV1I GCAGC 4 cut(s) 86, 286, 838, 848
BstX2I RGATCY 1 cut(s) 382
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 382
BstZ17I GTATAC 1 cut(s) 115
BstZI CGGCCG 1 cut(s) 991
Bsu15I ATCGAT 1 cut(s) 1191
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 4 cut(s) 214, 362, 993, 1098
BsuTUI ATCGAT 1 cut(s) 1191
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 550, 598, 973
BtsIMutI CAGTG 2 cut(s) 470, 1001
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 1 cut(s) 288
CfoI GCGC 2 cut(s) 543, 861
Cfr13I GGNCC 3 cut(s) 598, 1010, 1096
ClaI ATCGAT 1 cut(s) 1191
Csp6I GTAC 4 cut(s) 631, 795, 976, 1126
CviAII CATG 5 cut(s) 51, 287, 358, 500, 896
CviQI GTAC 4 cut(s) 631, 795, 976, 1126
DpnI GATC 9 cut(s) 384, 462, 733, 878, 942, 1023, 1032, 1190, 1194
DpnII GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
DraI TTTAAA 1 cut(s) 310
DraIII CACNNNGTG 1 cut(s) 816
DrdI GACNNNNNNGTC 1 cut(s) 757
DseDI GACNNNNNNGTC 1 cut(s) 757
EaeI YGGCCR 2 cut(s) 360, 991
EagI CGGCCG 1 cut(s) 991
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EclXI CGGCCG 1 cut(s) 991
Eco105I TACGTA 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 357
Eco32I GATATC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 598, 1010
Eco47III AGCGCT 1 cut(s) 542
Eco52I CGGCCG 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 999
Eco72I CACGTG 1 cut(s) 1036
Eco88I CYCGRG 2 cut(s) 237, 846
EcoO109I RGGNCCY 2 cut(s) 598, 1010
EcoRII CCWGG 1 cut(s) 854
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 5 cut(s) 54, 290, 361, 503, 899
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 1 cut(s) 918
FatI CATG 5 cut(s) 50, 286, 357, 499, 895
FauI CCCGC 1 cut(s) 1110
FbaI TGATCA 2 cut(s) 1021, 1030
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 5 cut(s) 75, 275, 852, 862, 994
FokI GGATG 3 cut(s) 537, 605, 980
Fsp4HI GCNGC 5 cut(s) 75, 275, 852, 862, 994
FspBI CTAG 2 cut(s) 345, 908
GlaI GCGC 2 cut(s) 542, 860
GluI GCNGC 5 cut(s) 75, 275, 852, 862, 994
HaeII RGCGCY 2 cut(s) 544, 862
HaeIII GGCC 4 cut(s) 214, 362, 993, 1098
HapII CCGG 2 cut(s) 71, 813
HhaI GCGC 2 cut(s) 543, 861
Hin1II CATG 5 cut(s) 54, 290, 361, 503, 899
Hin6I GCGC 2 cut(s) 541, 859
HinP1I GCGC 2 cut(s) 541, 859
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 6 cut(s) 32, 143, 202, 892, 1064, 1121
HpaII CCGG 2 cut(s) 71, 813
HphI GGTGA 1 cut(s) 1062
Hpy166II GTNNAC 3 cut(s) 115, 610, 932
Hpy188I TCNGA 5 cut(s) 130, 605, 697, 1018, 1148
Hpy188III TCNNGA 5 cut(s) 36, 266, 554, 880, 1171
Hpy8I GTNNAC 3 cut(s) 115, 610, 932
HpyAV CCTTC 6 cut(s) 312, 463, 647, 741, 1023, 1143
HpyCH4III ACNGT 2 cut(s) 630, 718
HpyCH4IV ACGT 2 cut(s) 633, 1035
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 547
HpySE526I ACGT 2 cut(s) 633, 1035
Hsp92II CATG 5 cut(s) 54, 290, 361, 503, 899
HspAI GCGC 2 cut(s) 541, 859
Ksp22I TGATCA 2 cut(s) 1021, 1030
Kzo9I GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 805, 838
Lsp1109I GCAGC 4 cut(s) 86, 286, 838, 848
LweI GCATC 5 cut(s) 319, 423, 559, 712, 958
MaeI CTAG 2 cut(s) 345, 908
MaeII ACGT 2 cut(s) 633, 1035
MaeIII GTNAC 6 cut(s) 16, 163, 485, 575, 1036, 1068
MalI GATC 9 cut(s) 384, 462, 733, 878, 942, 1023, 1032, 1190, 1194
MboI GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
MboII GAAGA 3 cut(s) 330, 334, 430
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 934
MluCI AATT 7 cut(s) 172, 311, 334, 473, 613, 711, 1050
MmeI TCCRAC 2 cut(s) 297, 1166
MroXI GAANNNNTTC 2 cut(s) 542, 1142
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 447
MspI CCGG 2 cut(s) 71, 813
MspR9I CCNGG 3 cut(s) 71, 814, 856
MvaI CCWGG 1 cut(s) 856
MwoI GCNNNNNNNGC 2 cut(s) 283, 547
NciI CCSGG 2 cut(s) 71, 814
NcoI CCATGG 1 cut(s) 357
NdeII GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
NlaIII CATG 5 cut(s) 54, 290, 361, 503, 899
NlaIV GGNNCC 1 cut(s) 834
NmeAIII GCCGAG 1 cut(s) 997
NmuCI GTSAC 4 cut(s) 163, 485, 1036, 1068
NspI RCATGY 1 cut(s) 290
PaeI GCATGC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 443
PdmI GAANNNNTTC 2 cut(s) 542, 1142
PfeI GAWTC 6 cut(s) 32, 143, 202, 892, 1064, 1121
PflFI GACNNNGTC 1 cut(s) 491
PkrI GCNGC 5 cut(s) 76, 276, 853, 863, 995
PmaCI CACGTG 1 cut(s) 1036
PmlI CACGTG 1 cut(s) 1036
Ppu21I YACGTR 2 cut(s) 634, 1036
PpuMI RGGWCCY 2 cut(s) 598, 1010
Psp5II RGGWCCY 2 cut(s) 598, 1010
Psp6I CCWGG 1 cut(s) 854
PspCI CACGTG 1 cut(s) 1036
PspGI CCWGG 1 cut(s) 854
PspN4I GGNNCC 1 cut(s) 834
PspPI GGNCC 3 cut(s) 598, 1010, 1096
PspPPI RGGWCCY 2 cut(s) 598, 1010
PspXI VCTCGAGB 1 cut(s) 237
PsrI GAACNNNNNNTAC 2 cut(s) 627, 659
PstI CTGCAG 1 cut(s) 511
PsuI RGATCY 1 cut(s) 382
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 4 cut(s) 632, 796, 977, 1127
RsaNI GTAC 4 cut(s) 631, 795, 976, 1126
RseI CAYNNNNRTG 1 cut(s) 447
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 5 cut(s) 75, 275, 852, 862, 994
Sau3AI GATC 9 cut(s) 382, 460, 731, 876, 940, 1021, 1030, 1188, 1192
Sau96I GGNCC 3 cut(s) 598, 1010, 1096
ScrFI CCNGG 3 cut(s) 71, 814, 856
SduI GDGCHC 1 cut(s) 934
SfaNI GCATC 5 cut(s) 319, 423, 559, 712, 958
SfcI CTRYAG 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 237
SinI GGWCC 2 cut(s) 598, 1010
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 2 cut(s) 237, 659
SmoI CTYRAG 2 cut(s) 237, 659
SnaBI TACGTA 1 cut(s) 634
SphI GCATGC 1 cut(s) 290
Sse9I AATT 7 cut(s) 172, 311, 334, 473, 613, 711, 1050
SsiI CCGC 3 cut(s) 42, 994, 1117
SspMI CTAG 2 cut(s) 345, 908
StyD4I CCNGG 3 cut(s) 69, 812, 854
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 630, 718
TaiI ACGT 2 cut(s) 636, 1038
TaqI TCGA 5 cut(s) 35, 238, 459, 943, 1191
TasI AATT 7 cut(s) 172, 311, 334, 473, 613, 711, 1050
TatI WGTACW 2 cut(s) 794, 975
TauI GCSGC 1 cut(s) 996
TfiI GAWTC 6 cut(s) 32, 143, 202, 892, 1064, 1121
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 470, 1008
TseFI GTSAC 4 cut(s) 163, 485, 1036, 1068
TseI GCWGC 4 cut(s) 74, 274, 851, 861
Tsp45I GTSAC 4 cut(s) 163, 485, 1036, 1068
TspDTI ATGAA 7 cut(s) 17, 135, 330, 488, 801, 884, 1151
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 2 cut(s) 470, 1008
Tth111I GACNNNGTC 1 cut(s) 491
VneI GTGCAC 1 cut(s) 930
VpaK11BI GGWCC 2 cut(s) 598, 1010
XapI RAATTY 3 cut(s) 334, 473, 1050
XceI RCATGY 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 2 cut(s) 542, 1142
XspI CTAG 2 cut(s) 345, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.