MD12G1259600.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012632
Physical Location & Seq
Forward (+)
32601372 .. 32604937
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1259600.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAAGTCCTCCTCCGTAACTTTACGTGCAGATCTGAGAAGCGGGAAATTGTGTTTTTGTAGCATTCTCCCGGCAGCTTCTCTGCTGTGTATACTGTTTCTCATAGTGAGTGCATACATATCATCAGATTACAGAGAGAATTCATCATCGTTTTGGAGAGTCACAAACAATTTGCAGAATCTAAAGTACAAATGCATGAATCAGAAAAAGCCTTATGGAAGTGAGCCACTGCCTCGAGGCATTGTTGCCAAGACTTCTGATCTGGAAATGCGGCCGTTATGGAGTGCTAAAAAGCCTTCAGTTAACTTGTTGAAGAAGAAAGATGCAAATTCCTCAACTAGTTTATTTGCCATGGCTGTCGGGATAAAGCAAAAGGATCTTGTTAGTAAAATGGTCAAAAAGTTCCTGTCTAGTGGTTTCGTTGTGATGCTCTTCCATTATGATGGCATCGTCGATGAGTGGAAGGAATTTCAATGGAGTGACCAAGTTATTCATGTATCTGCAGTCAGTCAAACTAAATGGTGGTTTGCAAAGCGTTTCTTGCATCCTGACATAGTTGCCGAATATAGTTACATTTTCCTCTGGGATGAGGACCTCGGAGTTGACTATTTCCAGCCCCAACGGTACATATCCATTGTTCAAAAGGAAGGGCTTGAGATATCACAACCTGCACTGGATTATTCAAAATCAGAGGTGCATCATCAAATTACTACGCGTTTGAGAGGATCGGTAGCACACAGAAGGACTTACAAGTCAAGTAACAATGGGAAAGGTTGTTATGAGAGCAGTAGAGCTCCTCCTTGCACCGGGTGGATAGAAGTGATGGCTCCTGTTTTCTCTCGAGCTGCCTGGCGTTGTGTTTGGTATATGATCCAGAATAACTTGATTCATGCTTGGGGACTAGATATGCAGATTGGTTATTGTGCACAGGGAGATCGAACTAAAAAAGTTGGAGTTGTGGATGCCGAGTACATAATCCATCACGGCCGCCCTACATTGGGAGGAGCCTCAGATGATCAAAATAATCAGGGATCATCATCATCATCCTCAAAATCTACAGGAAACGACCACAGAGTTGATGTGAGGCGGGAATCGTTCAATCAAATGAAAGTGTTCAGAAGGAAATGGGAACGAGCCGTCGGGAACGATAAATGTTGGATTGATCCCTACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

392

Amino Acids

44.71

Weight (kDa)

9.4

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 69 - 380 7.2e-139 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 751
AbsI CCTCGAGG 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 91
AccII CGCG 1 cut(s) 715
AciI CCGC 4 cut(s) 42, 271, 988, 1087
AclWI GGATC 5 cut(s) 384, 733, 865, 1039, 1157
AcoI YGGCCR 2 cut(s) 272, 985
AcsI RAATTY 3 cut(s) 139, 328, 467
AcuI CTGAAG 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 810
AfaI GTAC 3 cut(s) 188, 626, 971
AfiI CCNNNNNNNGG 3 cut(s) 806, 997, 998
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 5 cut(s) 313, 473, 641, 684, 1099
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 848
AluBI AGCT 3 cut(s) 77, 794, 845
AluI AGCT 3 cut(s) 77, 794, 845
Alw21I GWGCWC 2 cut(s) 796, 928
Alw44I GTGCAC 1 cut(s) 924
AlwI GGATC 5 cut(s) 384, 733, 865, 1039, 1157
Ama87I CYCGRG 2 cut(s) 234, 840
AoxI GGCC 2 cut(s) 272, 985
ApaLI GTGCAC 1 cut(s) 924
ApeKI GCWGC 2 cut(s) 74, 845
ApoI RAATTY 3 cut(s) 139, 328, 467
Asp700I GAANNNNTTC 1 cut(s) 1112
AspS9I GGNCC 1 cut(s) 592
AsuC2I CCSGG 2 cut(s) 71, 808
AvaI CYCGRG 2 cut(s) 234, 840
AvaII GGWCC 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 928
BanII GRGCYC 1 cut(s) 796
Bbv12I GWGCWC 2 cut(s) 796, 928
BbvI GCAGC 2 cut(s) 86, 832
BccI CCATC 3 cut(s) 437, 817, 987
BceAI ACGGC 3 cut(s) 259, 1000, 1121
BciT130I CCWGG 1 cut(s) 850
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 2 cut(s) 71, 808
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 3 cut(s) 339, 411, 902
BfmI CTRYAG 2 cut(s) 501, 1056
BfuAI ACCTGC 1 cut(s) 676
BglII AGATCT 1 cut(s) 31
BisI GCNGC 4 cut(s) 75, 272, 846, 988
BlsI GCNGC 4 cut(s) 76, 273, 847, 989
Bme1390I CCNGG 3 cut(s) 71, 808, 850
Bme18I GGWCC 1 cut(s) 592
BmeT110I CYCGRG 2 cut(s) 234, 840
BmgT120I GGNCC 1 cut(s) 592
BmiI GGNNCC 2 cut(s) 828, 1006
BmrFI CCNGG 3 cut(s) 71, 808, 850
BmsI GCATC 6 cut(s) 313, 417, 456, 553, 706, 952
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 2 cut(s) 71, 808
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 1036
BsaJI CCNNGG 2 cut(s) 351, 595
Bsc4I CCNNNNNNNGG 3 cut(s) 806, 997, 998
Bse1I ACTGG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 1036
BseBI CCWGG 1 cut(s) 850
BseDI CCNNGG 2 cut(s) 351, 595
BseGI GGATG 4 cut(s) 544, 592, 967, 1043
BseJI GATNNNNATC 1 cut(s) 1036
BseLI CCNNNNNNNGG 3 cut(s) 806, 997, 998
BseMII CTCAG 2 cut(s) 26, 1023
BseNI ACTGG 1 cut(s) 678
BseRI GAGGAG 2 cut(s) 786, 1017
BseSI GKGCMC 1 cut(s) 928
BseX3I CGGCCG 2 cut(s) 272, 985
BseXI GCAGC 2 cut(s) 86, 832
BsgI GTGCAG 2 cut(s) 48, 654
Bsh1236I CGCG 1 cut(s) 715
Bsh1285I CGRYCG 2 cut(s) 275, 988
BshFI GGCC 2 cut(s) 274, 987
BsiEI CGRYCG 2 cut(s) 275, 988
BsiHKAI GWGCWC 2 cut(s) 796, 928
BsiHKCI CYCGRG 2 cut(s) 234, 840
BsiSI CCGG 2 cut(s) 71, 807
BslFI GGGAC 1 cut(s) 912
BslI CCNNNNNNNGG 3 cut(s) 806, 997, 998
BsmFI GGGAC 1 cut(s) 912
BsmI GAATGC 1 cut(s) 63
BsnI GGCC 2 cut(s) 274, 987
BsoBI CYCGRG 2 cut(s) 234, 840
Bsp1286I GDGCHC 2 cut(s) 796, 928
Bsp143I GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 4 cut(s) 42, 271, 988, 1087
BspANI GGCC 2 cut(s) 274, 987
BspCNI CTCAG 2 cut(s) 27, 1022
BspFNI CGCG 1 cut(s) 715
BspLI GGNNCC 2 cut(s) 828, 1006
BspMAI CTGCAG 1 cut(s) 505
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 5 cut(s) 384, 733, 865, 1039, 1157
BspQI GCTCTTC 1 cut(s) 437
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 2 cut(s) 351, 595
BssMI GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
BssNAI GTATAC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 850
Bst4CI ACNGT 2 cut(s) 96, 624
Bst6I CTCTTC 1 cut(s) 437
BstBAI YACGTR 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 35, 1009
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 4 cut(s) 544, 592, 967, 1043
BstFNI CGCG 1 cut(s) 715
BstKTI GATC 9 cut(s) 34, 262, 379, 728, 873, 937, 1018, 1034, 1165
BstMBI GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
BstMCI CGRYCG 2 cut(s) 275, 988
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 1 cut(s) 850
BstSCI CCNGG 3 cut(s) 69, 806, 848
BstSFI CTRYAG 2 cut(s) 501, 1056
BstSLI GKGCMC 1 cut(s) 928
BstUI CGCG 1 cut(s) 715
BstV1I GCAGC 2 cut(s) 86, 832
BstX2I RGATCY 2 cut(s) 31, 376
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 2 cut(s) 31, 376
BstZ17I GTATAC 1 cut(s) 92
BstZI CGGCCG 2 cut(s) 272, 985
BsuRI GGCC 2 cut(s) 274, 987
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 4 cut(s) 544, 592, 967, 1043
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 227, 671
BveI ACCTGC 1 cut(s) 676
Cfr13I GGNCC 1 cut(s) 592
Csp6I GTAC 3 cut(s) 187, 625, 970
CviAII CATG 4 cut(s) 196, 352, 494, 890
CviQI GTAC 3 cut(s) 187, 625, 970
DdeI CTNAG 2 cut(s) 35, 1009
DpnI GATC 9 cut(s) 33, 261, 378, 727, 872, 936, 1017, 1033, 1164
DpnII GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
DraIII CACNNNGTG 1 cut(s) 810
DrdI GACNNNNNNGTC 1 cut(s) 751
DseDI GACNNNNNNGTC 1 cut(s) 751
EaeI YGGCCR 2 cut(s) 272, 985
EagI CGGCCG 2 cut(s) 272, 985
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 794
EclXI CGGCCG 2 cut(s) 272, 985
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 796
Eco32I GATATC 1 cut(s) 660
Eco47I GGWCC 1 cut(s) 592
Eco52I CGGCCG 2 cut(s) 272, 985
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 234, 840
EcoICRI GAGCTC 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 848
EcoRV GATATC 1 cut(s) 660
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 4 cut(s) 199, 355, 497, 893
FalI AAGNNNNNCTT 2 cut(s) 524, 556
FaqI GGGAC 1 cut(s) 912
FatI CATG 4 cut(s) 195, 351, 493, 889
FauI CCCGC 2 cut(s) 35, 1080
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 1 cut(s) 91
Fnu4HI GCNGC 4 cut(s) 75, 272, 846, 988
FokI GGATG 4 cut(s) 531, 599, 974, 1030
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 4 cut(s) 75, 272, 846, 988
FspBI CTAG 3 cut(s) 339, 411, 902
GluI GCNGC 4 cut(s) 75, 272, 846, 988
HaeIII GGCC 2 cut(s) 274, 987
HapII CCGG 2 cut(s) 71, 807
Hin1II CATG 4 cut(s) 199, 355, 497, 893
HincII GTYRAC 2 cut(s) 304, 604
HindII GTYRAC 2 cut(s) 304, 604
HinfI GANTC 5 cut(s) 159, 178, 199, 886, 1091
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 2 cut(s) 71, 807
Hpy166II GTNNAC 4 cut(s) 92, 304, 604, 926
Hpy188I TCNGA 8 cut(s) 36, 127, 204, 259, 599, 691, 1012, 1118
Hpy188III TCNNGA 6 cut(s) 263, 361, 548, 840, 874, 1141
Hpy8I GTNNAC 4 cut(s) 92, 304, 604, 926
Hpy99I CGWCG 2 cut(s) 455, 1142
HpyAV CCTTC 5 cut(s) 306, 457, 641, 735, 1113
HpyCH4III ACNGT 2 cut(s) 96, 624
HpyCH4IV ACGT 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 2 cut(s) 35, 1009
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 4 cut(s) 199, 355, 497, 893
Ksp22I TGATCA 1 cut(s) 1015
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 3 cut(s) 799, 832, 1004
Lsp1109I GCAGC 2 cut(s) 86, 832
LweI GCATC 6 cut(s) 313, 417, 456, 553, 706, 952
MaeI CTAG 3 cut(s) 339, 411, 902
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 5 cut(s) 16, 160, 479, 569, 758
MalI GATC 9 cut(s) 33, 261, 378, 727, 872, 936, 1017, 1033, 1164
MboI GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
MboII GAAGA 3 cut(s) 325, 328, 424
MflI RGATCY 2 cut(s) 31, 376
MhlI GDGCHC 2 cut(s) 796, 928
MluCI AATT 6 cut(s) 47, 139, 169, 328, 467, 705
MluI ACGCGT 1 cut(s) 713
MlyI GAGTC 1 cut(s) 168
MmeI TCCRAC 2 cut(s) 931, 1136
Mph1103I ATGCAT 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 1112
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 2 cut(s) 71, 807
MspR9I CCNGG 3 cut(s) 71, 808, 850
Mva1269I GAATGC 1 cut(s) 63
MvaI CCWGG 1 cut(s) 850
MvnI CGCG 1 cut(s) 715
MwoI GCNNNNNNNGC 1 cut(s) 541
NciI CCSGG 2 cut(s) 71, 808
NcoI CCATGG 1 cut(s) 351
NdeII GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
NlaIII CATG 4 cut(s) 199, 355, 497, 893
NlaIV GGNNCC 2 cut(s) 828, 1006
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 160, 479
NsiI ATGCAT 1 cut(s) 197
PaeR7I CTCGAG 2 cut(s) 234, 840
PciSI GCTCTTC 1 cut(s) 437
PctI GAATGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 1112
PfeI GAWTC 4 cut(s) 178, 199, 886, 1091
PkrI GCNGC 4 cut(s) 76, 273, 847, 989
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 592
Psp124BI GAGCTC 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 848
PspGI CCWGG 1 cut(s) 848
PspN4I GGNNCC 2 cut(s) 828, 1006
PspPI GGNCC 1 cut(s) 592
PspPPI RGGWCCY 1 cut(s) 592
PspXI VCTCGAGB 1 cut(s) 234
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 2 cut(s) 31, 376
RsaI GTAC 3 cut(s) 188, 626, 971
RsaNI GTAC 3 cut(s) 187, 625, 970
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 796
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 4 cut(s) 75, 272, 846, 988
Sau3AI GATC 9 cut(s) 31, 259, 376, 725, 870, 934, 1015, 1031, 1162
Sau96I GGNCC 1 cut(s) 592
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 3 cut(s) 71, 808, 850
SduI GDGCHC 2 cut(s) 796, 928
SetI ASST 8 cut(s) 28, 79, 597, 670, 696, 775, 796, 847
SfaNI GCATC 6 cut(s) 313, 417, 456, 553, 706, 952
SfcI CTRYAG 2 cut(s) 501, 1056
Sfr274I CTCGAG 2 cut(s) 234, 840
SinI GGWCC 1 cut(s) 592
SlaI CTCGAG 2 cut(s) 234, 840
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 3 cut(s) 234, 653, 840
SmoI CTYRAG 3 cut(s) 234, 653, 840
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 6 cut(s) 47, 139, 169, 328, 467, 705
SsiI CCGC 4 cut(s) 42, 271, 988, 1087
SspMI CTAG 3 cut(s) 339, 411, 902
SstI GAGCTC 1 cut(s) 796
StyD4I CCNGG 3 cut(s) 69, 806, 848
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 96, 624
TaiI ACGT 1 cut(s) 28
TaqI TCGA 4 cut(s) 235, 453, 841, 937
TasI AATT 6 cut(s) 47, 139, 169, 328, 467, 705
TatI WGTACW 2 cut(s) 186, 969
TauI GCSGC 2 cut(s) 274, 990
TfiI GAWTC 4 cut(s) 178, 199, 886, 1091
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 2 cut(s) 234, 678
TseFI GTSAC 2 cut(s) 160, 479
TseI GCWGC 2 cut(s) 74, 845
Tsp45I GTSAC 2 cut(s) 160, 479
TspDTI ATGAA 6 cut(s) 17, 132, 212, 482, 878, 1121
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 2 cut(s) 234, 678
VneI GTGCAC 1 cut(s) 924
VpaK11BI GGWCC 1 cut(s) 592
XapI RAATTY 3 cut(s) 139, 328, 467
XhoI CTCGAG 2 cut(s) 234, 840
XmiI GTMKAC 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 1112
XspI CTAG 3 cut(s) 339, 411, 902
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.