Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001073

Physical Location & Seq

Genomic Coordinates Reverse (-)

32197676 .. 32207455

Gene Structure 9780 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1247300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3885 bp

ATGGAGATCGACGGAGCCCAATTATCCGCTGACCCTGATCCTCTCCCATTCGCCAGAAGTTACCAGCTAGAAGCATTAGAGGCAGCAATTAAGAAGAACACCATTGTGTTCTTGGAGACTGGCTCTGGAAAGACTCTGATTGCCATTATGCTTCTCCGCAGCTACGCTTATTTGCTCCGCAAGCCTTCTCGTTTCATTGCCGTCTTCTTGGTTCCTAAAGTTGTCTTGGTCAAACAACAAGCTGAAGCTGTGAAAATGCACACCGACTTGAAAGTGGGTATGTATTGGGGAGACATGGGAGTCGACTTCTGGGATGCGGATATGTGGAAGCAACAATTAGAACAATATGAGGTGCTTGTGATGACTCCTGCAATATTGCTTAGTAATTTGAGGCATAGCTTCTTCAAACTAAGCATGATAAAGGTTTTAATTTTTGATGAGTGCCATCATGCCAGGGGTAACCACGACTATGCTTGCATAATGAAGGACTTCTTTCATCGTGAGCTACATTCCGGTGAATCTGAACTTCCAAGAATATTTGGAATGACTGCTTCCCCCATAAAATCAAAAGGTGGAAATTCAGAGTCATCGTATTGGAAAATAATTGATGAACTTGAGACCCTGATGAATTCAAAGGTGTATACATGTGCTAGTGAATCCGTGCTTGCTGAGTTTATACCACATTCAACTACAAAGTTCAAATATTACAGGCATGAGGAGATTCCAGGTGGCTTGGTTGCAAATATAATAAACCAGTTGAATGCTTTGAAAGAAAAGCATGAACTGTCACTAGAAAGTTTGGATCTCAGTCAATCTACATCACAATCCATAAGCAATAAGATGAAGAAATTTTATTCAGCTTTAACATTTTGTTTGGAAGAACTTGGTGTTTGGCTGGCTTTAAAGGCTGCTTGGTTCTTTTCCCAGAAAGAAAATGAATTGTTTTCATGGGGGAAACTGGATGTGTTGGGCGAGAAAATTGTGAGAAACTTCAGTTCTGAAGCTTACCAGGCATTTGCATCTTTTTTACCATCTGATCGAAAGTGGACCATTGCGAATGACGTTACATACGATTTGGATAAGGGGCTTCTGACTTCAAAAGTTGTCTGTCTCATCCAATTGCTTCACGAATACAGGGGCTTAAAGGATTTGAGATGTATAGTTTTTGTTGAAAGGGTCATAACAGCTGTTGTCATCGAATCGTTATTTAGTACATTTTTTCCTAAACTCAATGACTGGAAGACAAAGTACATTGCGGGAAATAACAATGCGGTGCAATTCCAAACCAGAAAAAGGCAGAACGAAATTGTTGAAGAATTCCGTAAGGGCATGGTGAATATCATTGTTGCAACATCGATTCTTGAAGAGGGGTTAGATGTTCAAAGCTGTAATTTGGTCATTAGATTTGATCCTTCCTCCACTGTTTCTAGTTTCATACAGTCTAAAGGTCGTGCAAGGATGCAAAACTCTGATTATGTGTTAATGTTGAAAAGTGGTGATTCCAGTACACATTCTCGAGTACAAAATTATCTTGCAAGTGGAGCTATAATGAGAAAGGAGTCCTTACGCCATTCTTCTCTTGCCTGCACACCTTTGGAAATTGATTTGCAGGATGATGACTTTTATCGTGTTGAAAGTACTGGAGCAAGCTTGAGCCTTGCTTCCTCTATTGGATTGATGTACTTCTATTGCTCAAGGCTCCCTTCTGATGGATATTTTAAACCTGCTCCAAGATGGGACAAGGAGACTTGCACTTTGCATCTTCCCAAGAGCTGTCCAATACCTGATGTTCATGTAAAAGGAAATGTTAAAATTTTGAAGCAACTTGCATGCTTCGAAGCTTGCAAGGAACTTCACAAAATTGGTGCTTTAACGGATAATCTTGTTCCAGATATTGTTGAGGAAGAAGGCACACAAGAGCTTGGATCGGAACCGTACGATGATGTACAGTGCAGCTATGTCCCAATTGAATTAGTCAAGCCCTTTTGCTCAAATGATCCTAGTATATTGTATCATTGCTACTTAATTGAGCTGAATCAGAATTTTGAATATGATATTCCAGTTCATAACATTGTACTTGGTATGAGGAGTGAGCTGGATTGCGATATTGCAAATCCTCATTTTGACTTAGAAGTCGGCAGAGGTAGTGTAACAGTAAACTTTAAATATGTTGGAGAAATTCATCTAAATTCAGAACAGGTACTTCTGTGTAGAAGGTTCCAAATCACAATTTTTCAAATTCTAATTGATCACAATTTGAATAAGCTGGGAGAAGTTTTAGATGGACTTTCTTCGGGAGGAAATGTTGGGATTGATTATCTTTTACTCCCGGGGACCAGAATACATCAGGAAGCTGTGATCATTGATTGGAGATGTGTTACATCTGTGTTATTTGCATCTGAAGAATATTCCAATGATCACGTAAATTGTTCAACGCTTAACGGCTGTTCCCGTTTGGTACATACCAAACAGGGTTTGGTTTGTACATGCATGATCCAGAATTCTTTGGTTTGTACCCCTCATAATGGTACCATATATTGCATCACTGGTTTGTTGAGTAACTTGAATGGAAACTCACTTCTTAAGCTCAGGGATGGCAGAGCTCTTACGTATAAGAAATACTACGAAGAAAGACACAAGATCAACTTGTATTTTGATCAACAATTGCTACTGAAAGGGAGACGAATTTTCCAGGTGCAAAATTATCTACAAAGGTGCAGACAACAGAAAGAAAAAGAATCGAGTCATACATCTGTTGAATTGCCACCTGAATTTTGTTCTATAATTATGTCACCTATATCAATCAGCAGTGTATATTCATACTCATTTGTTCCTTCAATCATGCATCAACTTGAGGCTTTTCTTATTGCTGCCAACTTGAAAAGGATTGTTTTGGATCATTGCACACAAAATGTTATCATTCCAACCGTTAAGGTCTTGGAAGCTATTACAACAAAAAAATGTCAAGAGAATTACCATTTGGAATCCTTGGAGGCTCTTGGAGATTCATTTCTTAAATATGCTGCTAGTCAGCAGCTTTTCAAAACATATCAGAACAATCATGAAGGCCTTCTTAGTGTAAAGAAGGAAAAGATAATTTCTAATGCTGCCCTTTGCAGGCTTGGCTGTGACCACAAACTTCCGGGCTTTATTCGTAACGAGAGTTTTGACCCAAAAAATTGGTTTATACCTGGAGATTATTCTGGAAGTTATTTACTATATGAGGAGTTGCTTTATGACGGAAGAAAGATCTACACTAGGGAAAGACGGAAGGTAAAAAGTAAGAGTGTTGCTGATGTGGTTGAAGCACTAATTGGTGCATTTCTTAGCACAGGTGGGGAAGTAGCTGCCATGTATTTCATGAATTGGGTTGGTATAAAGGTGGATTTTGTCCATATACCATGCGAGAGGCACTTCCAACTGCAACCGGAGAAGCTTGTCAATGTCGGACGTATAGAGTCCTTACTGAAGTATTCATTCCGTGATCCTTCTCTTCTTGTTGAGGCACTAACCCATGGTTCGTACATGCTTCCTGAGATTCCAGGATGTTATCAGCGGCTGGAATTTCTCGGGGATGCTGTGTTAGATTATCTGATCACTATATATTTGTACAATGAGTATCCTGGGATGTCACCGGGAATCTTAACTGATATGAGGTCTGCCTCTGTGAACAACGACTGTTATGCTCGCTCTGCTATTAAGGCTGAGTTGTACAAGCACATTCTGCATGCTTCACACAAACTGCACAAAGATATAGTTTACACAATTGAAAACTTTGAGAGGTTATCTACGGAATCGACTTTTGGATGGGATTCCGAGACATCATTCCCCAAGGTTAGTACTTGGAGATGTTGTCGAGTCTCTAGCAGGGGCAATTTATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1295

Amino Acids

147.18

Weight (kDa)

6.38

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	19 - 187	1.2e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	20 - 187	6.4e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	367 - 485	2.1e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	556 - 631	8.2e-14	Dicer dimerisation domain
PAZ	PF02170	824 - 934	7.5e-12	PAZ domain
Ribonuclease_3	PF00636	995 - 1112	1e-17	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1162 - 1243	7.7e-11	Ribonuclease-III-like
Ribonuclease_3	PF00636	1185 - 1261	6.3e-15	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001073)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03300 AT3G03300 AT3G03300 AT3G03300
fragaria_vesca	FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g00200 FvH4_6g37440 FvH4_6g37440 FvH4_6g37440
malus_domestica	MD04G1247300.v1.1 MD12G1087600.v1.1 MD14G1082600.v1.1 MD14G1082800.v1.1
prunus_persica	Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.6G363600_v2.0.a1 Prupe.7G047700_v2.0.a1 Prupe.7G047900_v2.0.a1 Prupe.7G047900_v2.0.a1 Prupe.7G047900_v2.0.a1 Prupe.7G047900_v2.0.a1 Prupe.7G048000_v2.0.a1 Prupe.7G048000_v2.0.a1 Prupe.7G048000_v2.0.a1 Prupe.7G048000_v2.0.a1
pyrus_communis	pycom04g21800 pycom12g07520 pycom14g07170
rosa_chinensis	RchiOBHm_Chr3g0447571
rosa_laevigata	RLG00000020482 RLG00000025988
rosa_multiflora	Rmu_sc0003550.1_g000007
rosa_roxburghii	Rroxscaffold_5G00355920
rosa_rugosa	Rorug02G0603000 Rorug02G0603100
rosa_samantha	Rh3AG003300 Rh3BG002800 Rh3CG003100 Rh3DG003100
rosa_wichuraiana	Rw3G000220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	299, 2132
Acc36I	ACCTGC	1 cut(s)	1732
Acc65I	GGTACC	1 cut(s)	2528
AccB1I	GGYRCC	1 cut(s)	2528
AccI	GTMKAC	2 cut(s)	303, 641
AciI	CCGC	7 cut(s)	27, 157, 178, 317, 1256, 1271, 3556
AclWI	GGATC	8 cut(s)	32, 810, 1403, 1933, 1991, 2488, 2904, 3479
AcuI	CTGAAG	5 cut(s)	264, 976, 1020, 2421, 3488
AfiI	CCNNNNNNNGG	4 cut(s)	1293, 1709, 2525, 3117
AflII	CTTAAG	1 cut(s)	2582
AflIII	ACRYGT	1 cut(s)	644
AjnI	CCWGG	7 cut(s)	452, 724, 1008, 2691, 3190, 3541, 3622
AjuI	GAANNNNNNNTTGG	6 cut(s)	2963, 2995, 3297, 3329, 3786, 3818
AleI	CACNNNNGTG	1 cut(s)	104
Alw21I	GWGCWC	1 cut(s)	2605
Alw26I	GTCTC	8 cut(s)	110, 285, 611, 1115, 1739, 2674, 3812, 3865
AlwI	GGATC	8 cut(s)	32, 810, 1403, 1933, 1991, 2488, 2904, 3479
AlwNI	CAGNNNCTG	1 cut(s)	3559
Ama87I	CYCGRG	3 cut(s)	1515, 2329, 3569
AoxI	GGCC	1 cut(s)	3067
ApeKI	GCWGC	9 cut(s)	83, 159, 908, 1953, 2870, 3023, 3034, 3107, 3347
ArsI	GACNNNNNNTTYG	2 cut(s)	1738, 1770
Asp700I	GAANNNNTTC	2 cut(s)	488, 3069
Asp718I	GGTACC	1 cut(s)	2528
AspS9I	GGNCC	2 cut(s)	1047, 2334
AsuC2I	CCSGG	4 cut(s)	2330, 2331, 3144, 3636
AsuHPI	GGTGA	5 cut(s)	527, 1345, 1508, 2784, 3624
AsuII	TTCGAA	1 cut(s)	1836
AvaI	CYCGRG	3 cut(s)	1515, 2329, 3569
AvaII	GGWCC	2 cut(s)	1047, 2334
BaeI	ACNNNNGTAYC	2 cut(s)	2192, 2225
BanI	GGYRCC	1 cut(s)	2528
BanII	GRGCYC	2 cut(s)	19, 2605
BarI	GAAGNNNNNNTAC	2 cut(s)	1232, 1264
BbsI	GAAGAC	2 cut(s)	196, 1247
Bbv12I	GWGCWC	1 cut(s)	2605
BbvI	GCAGC	9 cut(s)	95, 171, 895, 1965, 2857, 3010, 3046, 3094, 3334
BccI	CCATC	7 cut(s)	453, 1039, 1703, 1728, 2276, 2588, 3801
BceAI	ACGGC	2 cut(s)	185, 2458
BcgI	CGANNNNNNTGC	2 cut(s)	3665, 3699
BciT130I	CCWGG	7 cut(s)	454, 726, 1010, 2693, 3192, 3543, 3624
BciVI	GTATCC	1 cut(s)	3630
BclI	TGATCA	5 cut(s)	2248, 2358, 2416, 2656, 3594
BcnI	CCSGG	4 cut(s)	2330, 2331, 3144, 3636
BcoDI	GTCTC	8 cut(s)	110, 285, 611, 1115, 1739, 2674, 3812, 3865
BfaI	CTAG	8 cut(s)	68, 651, 791, 1428, 2001, 3027, 3258, 3864
BfrI	CTTAAG	1 cut(s)	2582
BfuAI	ACCTGC	1 cut(s)	1732
BfuI	GTATCC	1 cut(s)	3630
BglII	AGATCT	1 cut(s)	3249
BmcAI	AGTACT	2 cut(s)	1639, 3841
Bme18I	GGWCC	2 cut(s)	1047, 2334
BmeT110I	CYCGRG	3 cut(s)	1515, 2329, 3569
BmgT120I	GGNCC	2 cut(s)	1047, 2334
BmiI	GGNNCC	7 cut(s)	16, 213, 1700, 1932, 2219, 2335, 2530
BmsI	GCATC	8 cut(s)	304, 1028, 1449, 1768, 2405, 2550, 2854, 3565
BpiI	GAAGAC	2 cut(s)	196, 1247
BplI	GAGNNNNNCTC	2 cut(s)	107, 139
BpmI	CTGGAG	2 cut(s)	1662, 3213
Bpu10I	CCTNAGC	1 cut(s)	2588
Bpu14I	TTCGAA	1 cut(s)	1836
BpuEI	CTTGAG	4 cut(s)	635, 1672, 1678, 2873
BpuMI	CCSGG	4 cut(s)	2330, 2331, 3144, 3636
Bsa29I	ATCGAT	1 cut(s)	1355
BsaAI	YACGTR	2 cut(s)	2422, 2610
BsaI	GGTCTC	1 cut(s)	611
BsaJI	CCNNGG	7 cut(s)	453, 2329, 2330, 2988, 3514, 3623, 3831
BsaWI	WCCGGW	2 cut(s)	512, 3427
BsaXI	ACNNNNNCTCC	4 cut(s)	2289, 2319, 3838, 3868
Bsc4I	CCNNNNNNNGG	4 cut(s)	1293, 1709, 2525, 3117
Bse1I	ACTGG	8 cut(s)	124, 754, 963, 1241, 1503, 1645, 2060, 2551
Bse3DI	GCAATG	5 cut(s)	195, 1050, 1251, 2014, 2899
BseBI	CCWGG	7 cut(s)	454, 726, 1010, 2693, 3192, 3543, 3624
BseCI	ATCGAT	1 cut(s)	1355
BseDI	CCNNGG	7 cut(s)	453, 2329, 2330, 2988, 3514, 3623, 3831
BseLI	CCNNNNNNNGG	4 cut(s)	1293, 1709, 2525, 3117
BseMI	GCAATG	5 cut(s)	195, 1050, 1251, 2014, 2899
BseMII	CTCAG	5 cut(s)	660, 820, 2602, 3525, 3696
BseNI	ACTGG	8 cut(s)	124, 754, 963, 1241, 1503, 1645, 2060, 2551
BseRI	GAGGAG	3 cut(s)	731, 2101, 3239
BseXI	GCAGC	9 cut(s)	95, 171, 895, 1965, 2857, 3010, 3046, 3094, 3334
BseYI	CCCAGC	1 cut(s)	2266
BsgI	GTGCAG	4 cut(s)	1570, 1972, 2737, 3728
BshFI	GGCC	1 cut(s)	3069
BshNI	GGYRCC	1 cut(s)	2528
BshVI	ATCGAT	1 cut(s)	1355
BsiHKAI	GWGCWC	1 cut(s)	2605
BsiHKCI	CYCGRG	3 cut(s)	1515, 2329, 3569
BsiSI	CCGG	5 cut(s)	513, 2330, 3143, 3428, 3635
BsiWI	CGTACG	1 cut(s)	1935
BslFI	GGGAC	3 cut(s)	1751, 1946, 2347
BslI	CCNNNNNNNGG	4 cut(s)	1293, 1709, 2525, 3117
BsmAI	GTCTC	8 cut(s)	110, 285, 611, 1115, 1739, 2674, 3812, 3865
BsmBI	CGTCTC	1 cut(s)	2674
BsmFI	GGGAC	3 cut(s)	1751, 1946, 2347
BsmI	GAATGC	1 cut(s)	766
BsnI	GGCC	1 cut(s)	3069
Bso31I	GGTCTC	1 cut(s)	611
BsoBI	CYCGRG	3 cut(s)	1515, 2329, 3569
Bsp119I	TTCGAA	1 cut(s)	1836
Bsp1286I	GDGCHC	2 cut(s)	19, 2605
Bsp1407I	TGTACA	4 cut(s)	1945, 2483, 3609, 3711
Bsp19I	CCATGG	1 cut(s)	3514
BspACI	CCGC	7 cut(s)	27, 157, 178, 317, 1256, 1271, 3556
BspANI	GGCC	1 cut(s)	3069
BspCNI	CTCAG	5 cut(s)	661, 819, 2601, 3526, 3697
BspDI	ATCGAT	1 cut(s)	1355
BspHI	TCATGA	2 cut(s)	3061, 3360
BspLI	GGNNCC	7 cut(s)	16, 213, 1700, 1932, 2219, 2335, 2530
BspMI	ACCTGC	1 cut(s)	1732
BspPI	GGATC	8 cut(s)	32, 810, 1403, 1933, 1991, 2488, 2904, 3479
BspT104I	TTCGAA	1 cut(s)	1836
BspT107I	GGYRCC	1 cut(s)	2528
BspTI	CTTAAG	1 cut(s)	2582
BspTNI	GGTCTC	1 cut(s)	611
BsrDI	GCAATG	5 cut(s)	195, 1050, 1251, 2014, 2899
BsrGI	TGTACA	4 cut(s)	1945, 2483, 3609, 3711
BsrI	ACTGG	8 cut(s)	124, 754, 963, 1241, 1503, 1645, 2060, 2551
BssECI	CCNNGG	7 cut(s)	453, 2329, 2330, 2988, 3514, 3623, 3831
BssNAI	GTATAC	1 cut(s)	642
BssT1I	CCWWGG	3 cut(s)	2988, 3514, 3831
Bst1107I	GTATAC	1 cut(s)	642
Bst2UI	CCWGG	7 cut(s)	454, 726, 1010, 2693, 3192, 3543, 3624
Bst4CI	ACNGT	8 cut(s)	786, 1423, 1440, 1935, 1950, 2155, 2929, 3680
Bst6I	CTCTTC	2 cut(s)	1359, 3498
BstAFI	CTTAAG	1 cut(s)	2582
BstAPI	GCANNNNNTGC	1 cut(s)	3724
BstAUI	TGTACA	4 cut(s)	1945, 2483, 3609, 3711
BstBAI	YACGTR	2 cut(s)	2422, 2610
BstBI	TTCGAA	1 cut(s)	1836
BstDSI	CCRYGG	1 cut(s)	3514
BstEII	GGTNACC	1 cut(s)	458
BstMAI	GTCTC	8 cut(s)	110, 285, 611, 1115, 1739, 2674, 3812, 3865
BstMWI	GCNNNNNNNGC	9 cut(s)	80, 181, 905, 1010, 1541, 3123, 3692, 3701, 3724
BstNI	CCWGG	7 cut(s)	454, 726, 1010, 2693, 3192, 3543, 3624
BstNSI	RCATGY	5 cut(s)	648, 1833, 2490, 3529, 3731
BstPI	GGTNACC	1 cut(s)	458
BstSNI	TACGTA	1 cut(s)	2610
BstV1I	GCAGC	9 cut(s)	95, 171, 895, 1965, 2857, 3010, 3046, 3094, 3334
BstV2I	GAAGAC	2 cut(s)	196, 1247
BstX2I	RGATCY	2 cut(s)	802, 3249
BstXI	CCANNNNNNTGG	1 cut(s)	3180
BstYI	RGATCY	2 cut(s)	802, 3249
BstZ17I	GTATAC	1 cut(s)	642
Bsu15I	ATCGAT	1 cut(s)	1355
BsuI	GTATCC	1 cut(s)	3630
BsuRI	GGCC	1 cut(s)	3069
BsuTUI	ATCGAT	1 cut(s)	1355
BtgI	CCRYGG	1 cut(s)	3514
BtsI	GCAGTG	1 cut(s)	2815
BtsIMutI	CAGTG	4 cut(s)	1419, 1955, 2544, 2815
BveI	ACCTGC	1 cut(s)	1732
CaiI	CAGNNNCTG	1 cut(s)	3559
CciI	TCATGA	2 cut(s)	3061, 3360
Cfr13I	GGNCC	2 cut(s)	1047, 2334
Cfr9I	CCCGGG	1 cut(s)	2329
ClaI	ATCGAT	1 cut(s)	1355
DraI	TTTAAA	3 cut(s)	903, 1720, 2164
DrdI	GACNNNNNNGTC	2 cut(s)	299, 2132
DseDI	GACNNNNNNGTC	2 cut(s)	299, 2132
Eam1104I	CTCTTC	2 cut(s)	1359, 3498
EarI	CTCTTC	2 cut(s)	1359, 3498
Ecl136II	GAGCTC	1 cut(s)	2603
Eco105I	TACGTA	1 cut(s)	2610
Eco130I	CCWWGG	3 cut(s)	2988, 3514, 3831
Eco147I	AGGCCT	1 cut(s)	3069
Eco24I	GRGCYC	2 cut(s)	19, 2605
Eco31I	GGTCTC	1 cut(s)	611
Eco47I	GGWCC	2 cut(s)	1047, 2334
Eco53kI	GAGCTC	1 cut(s)	2603
Eco57I	CTGAAG	5 cut(s)	264, 976, 1020, 2421, 3488
Eco88I	CYCGRG	3 cut(s)	1515, 2329, 3569
Eco91I	GGTNACC	1 cut(s)	458
EcoICRI	GAGCTC	1 cut(s)	2603
EcoO65I	GGTNACC	1 cut(s)	458
EcoRI	GAATTC	3 cut(s)	628, 1316, 2500
EcoRII	CCWGG	7 cut(s)	452, 724, 1008, 2691, 3190, 3541, 3622
EcoT14I	CCWWGG	3 cut(s)	2988, 3514, 3831
EcoT22I	ATGCAT	2 cut(s)	2492, 2847
EcoT38I	GRGCYC	2 cut(s)	19, 2605
ErhI	CCWWGG	3 cut(s)	2988, 3514, 3831
Esp3I	CGTCTC	1 cut(s)	2674
FaqI	GGGAC	3 cut(s)	1751, 1946, 2347
FauI	CCCGC	1 cut(s)	1249
FbaI	TGATCA	5 cut(s)	2248, 2358, 2416, 2656, 3594
FblI	GTMKAC	2 cut(s)	303, 641
FriOI	GRGCYC	2 cut(s)	19, 2605
FspBI	CTAG	8 cut(s)	68, 651, 791, 1428, 2001, 3027, 3258, 3864
GsaI	CCCAGC	1 cut(s)	2270
GsuI	CTGGAG	2 cut(s)	1662, 3213
HaeIII	GGCC	1 cut(s)	3069
HapII	CCGG	5 cut(s)	513, 2330, 3143, 3428, 3635
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HindIII	AAGCTT	4 cut(s)	1002, 1648, 1839, 3434
HpaII	CCGG	5 cut(s)	513, 2330, 3143, 3428, 3635
HphI	GGTGA	5 cut(s)	527, 1345, 1508, 2784, 3624
Hpy166II	GTNNAC	7 cut(s)	304, 642, 1047, 1508, 2158, 3670, 3760
Hpy8I	GTNNAC	7 cut(s)	304, 642, 1047, 1508, 2158, 3670, 3760
Hpy99I	CGWCG	1 cut(s)	14
HpyCH4III	ACNGT	8 cut(s)	786, 1423, 1440, 1935, 1950, 2155, 2929, 3680
HpyCH4IV	ACGT	4 cut(s)	1062, 2421, 2609, 3451
HpyF10VI	GCNNNNNNNGC	9 cut(s)	80, 181, 905, 1010, 1541, 3123, 3692, 3701, 3724
HpySE526I	ACGT	4 cut(s)	1062, 2421, 2609, 3451
KpnI	GGTACC	1 cut(s)	2532
Ksp22I	TGATCA	5 cut(s)	2248, 2358, 2416, 2656, 3594
LmnI	GCTCC	6 cut(s)	14, 180, 1541, 1643, 1704, 1732
Lsp1109I	GCAGC	9 cut(s)	95, 171, 895, 1965, 2857, 3010, 3046, 3094, 3334
LweI	GCATC	8 cut(s)	304, 1028, 1449, 1768, 2405, 2550, 2854, 3565
MaeI	CTAG	8 cut(s)	68, 651, 791, 1428, 2001, 3027, 3258, 3864
MaeII	ACGT	4 cut(s)	1062, 2421, 2609, 3451
MfeI	CAATTG	4 cut(s)	1118, 1965, 2663, 3765
MflI	RGATCY	2 cut(s)	802, 3249
MhlI	GDGCHC	2 cut(s)	19, 2605
MlyI	GAGTC	8 cut(s)	127, 309, 358, 593, 1568, 2752, 3467, 3867
MmeI	TCCRAC	4 cut(s)	2152, 2948, 3427, 3442
Mph1103I	ATGCAT	2 cut(s)	2492, 2847
MroXI	GAANNNNTTC	2 cut(s)	488, 3069
MslI	CAYNNNNRTG	3 cut(s)	104, 468, 513
MspA1I	CMGCKG	3 cut(s)	29, 1187, 3556
MspCI	CTTAAG	1 cut(s)	2582
MspI	CCGG	5 cut(s)	513, 2330, 3143, 3428, 3635
MunI	CAATTG	4 cut(s)	1118, 1965, 2663, 3765
Mva1269I	GAATGC	1 cut(s)	766
MvaI	CCWGG	7 cut(s)	454, 726, 1010, 2693, 3192, 3543, 3624
MwoI	GCNNNNNNNGC	9 cut(s)	80, 181, 905, 1010, 1541, 3123, 3692, 3701, 3724
NciI	CCSGG	4 cut(s)	2330, 2331, 3144, 3636
NcoI	CCATGG	1 cut(s)	3514
NlaIV	GGNNCC	7 cut(s)	16, 213, 1700, 1932, 2219, 2335, 2530
NmuCI	GTSAC	4 cut(s)	786, 2790, 3128, 3630
NsiI	ATGCAT	2 cut(s)	2492, 2847
NspI	RCATGY	5 cut(s)	648, 1833, 2490, 3529, 3731
NspV	TTCGAA	1 cut(s)	1836
OliI	CACNNNNGTG	1 cut(s)	104
PaeI	GCATGC	2 cut(s)	1833, 3731
PaeR7I	CTCGAG	1 cut(s)	1515
PagI	TCATGA	2 cut(s)	3061, 3360
PceI	AGGCCT	1 cut(s)	3069
PciI	ACATGT	1 cut(s)	644
PcsI	WCGNNNNNNNCGW	1 cut(s)	1068
PctI	GAATGC	1 cut(s)	766
PdmI	GAANNNNTTC	2 cut(s)	488, 3069
Pfl23II	CGTACG	1 cut(s)	1935
PfoI	TCCNGGA	1 cut(s)	3541
PleI	GAGTC	8 cut(s)	127, 308, 358, 592, 1567, 2751, 3466, 3866
PpsI	GAGTC	8 cut(s)	127, 308, 358, 592, 1567, 2751, 3466, 3866
Ppu21I	YACGTR	2 cut(s)	2422, 2610
PscI	ACATGT	1 cut(s)	644
Psp124BI	GAGCTC	1 cut(s)	2605
Psp6I	CCWGG	7 cut(s)	452, 724, 1008, 2691, 3190, 3541, 3622
PspEI	GGTNACC	1 cut(s)	458
PspFI	CCCAGC	1 cut(s)	2266
PspGI	CCWGG	7 cut(s)	452, 724, 1008, 2691, 3190, 3541, 3622
PspLI	CGTACG	1 cut(s)	1935
PspN4I	GGNNCC	7 cut(s)	16, 213, 1700, 1932, 2219, 2335, 2530
PspPI	GGNCC	2 cut(s)	1047, 2334
PstNI	CAGNNNCTG	1 cut(s)	3559
PsuI	RGATCY	2 cut(s)	802, 3249
PvuII	CAGCTG	1 cut(s)	1187
RseI	CAYNNNNRTG	3 cut(s)	104, 468, 513
SacI	GAGCTC	1 cut(s)	2605
SalI	GTCGAC	1 cut(s)	302
Sau96I	GGNCC	2 cut(s)	1047, 2334
ScaI	AGTACT	2 cut(s)	1639, 3841
SchI	GAGTC	8 cut(s)	127, 309, 358, 593, 1568, 2752, 3467, 3867
SduI	GDGCHC	2 cut(s)	19, 2605
SfaNI	GCATC	8 cut(s)	304, 1028, 1449, 1768, 2405, 2550, 2854, 3565
Sfr274I	CTCGAG	1 cut(s)	1515
SfuI	TTCGAA	1 cut(s)	1836
SinI	GGWCC	2 cut(s)	1047, 2334
SlaI	CTCGAG	1 cut(s)	1515
SmaI	CCCGGG	1 cut(s)	2331
SmiMI	CAYNNNNRTG	3 cut(s)	104, 468, 513
SmlI	CTYRAG	6 cut(s)	614, 1515, 1651, 1693, 2582, 2852
SmoI	CTYRAG	6 cut(s)	614, 1515, 1651, 1693, 2582, 2852
SnaBI	TACGTA	1 cut(s)	2610
SphI	GCATGC	2 cut(s)	1833, 3731
SseBI	AGGCCT	1 cut(s)	3069
SsiI	CCGC	7 cut(s)	27, 157, 178, 317, 1256, 1271, 3556
SspI	AATATT	4 cut(s)	375, 537, 704, 2408
SspMI	CTAG	8 cut(s)	68, 651, 791, 1428, 2001, 3027, 3258, 3864
SstI	GAGCTC	1 cut(s)	2605
StuI	AGGCCT	1 cut(s)	3069
StyI	CCWWGG	3 cut(s)	2988, 3514, 3831
TaaI	ACNGT	8 cut(s)	786, 1423, 1440, 1935, 1950, 2155, 2929, 3680
TaiI	ACGT	4 cut(s)	1065, 2424, 2612, 3454
TauI	GCSGC	1 cut(s)	3559
TscAI	CASTG	4 cut(s)	1426, 1955, 2551, 2815
TseFI	GTSAC	4 cut(s)	786, 2790, 3128, 3630
TseI	GCWGC	9 cut(s)	83, 159, 908, 1953, 2870, 3023, 3034, 3107, 3347
Tsp45I	GTSAC	4 cut(s)	786, 2790, 3128, 3630
TspGWI	ACGGA	8 cut(s)	27, 649, 1310, 1889, 3255, 3283, 3470, 3806
TspMI	CCCGGG	1 cut(s)	2329
TspRI	CASTG	4 cut(s)	1426, 1955, 2551, 2815
Vha464I	CTTAAG	1 cut(s)	2582
VpaK11BI	GGWCC	2 cut(s)	1047, 2334
XceI	RCATGY	5 cut(s)	648, 1833, 2490, 3529, 3731
XcmI	CCANNNNNNNNNTGG	2 cut(s)	109, 2473
XhoI	CTCGAG	1 cut(s)	1515
XmaI	CCCGGG	1 cut(s)	2329
XmiI	GTMKAC	2 cut(s)	303, 641
XmnI	GAANNNNTTC	2 cut(s)	488, 3069
XspI	CTAG	8 cut(s)	68, 651, 791, 1428, 2001, 3027, 3258, 3864
ZrmI	AGTACT	2 cut(s)	1639, 3841
Zsp2I	ATGCAT	2 cut(s)	2492, 2847

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.