Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008801

Physical Location & Seq

Genomic Coordinates Reverse (-)

1179443 .. 1187642

Gene Structure 8200 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGCGGAGCTGCCAGTGATAACAATAGGAGGCAAGGGCTCGTCTCTGTCCTCCTCCTCCGTCTGCTCCCTCGCCAAGGGCCTTGCTCATCTGCGCACCGACTTGTCGGCGCTCAATCGGCTCGACTCCTCCTCCTCCTCCTCATCGCCCCTCGCCCACAATCTCGTCTCTCTCCTCCCCTCCCTCCCAACTGTCGAAGAATGCAGAGCCTCCCTCGTCGTCCTCCTCTGCAAGCTTTTATCTCTCTCCGGTTCCCCCAACGTCCGTACGGTTCTCGCGGTACGTCTCGCCGAAGCCATCAATTCCCGAGACTTGGAGCTCGGGTCGCTCGTTTTGACCGAAGAGGAGGCTCTTGCGTTGGAGGAATTCAAGTTATCCTCAGCTTTGTGCGGGATATGTGCGCTTCTGGACCACCAGTCCGCCGCGTTGTCTACTGTTTCCGATGCGGTGGCAGCGATGTCTTGCGAGGCGTTGAAGGCCGATGTAAGGGCGTTTAATTCGATGGATTTGGGGGATGGGTACGCAGCGAAGGAGGTGATTGCTGTGGCCAATGATTTGAAGGTGCTGCTCAATGGGTCTAAATTAGTGGGTAAGCTTGAAATTGAAGCGGTATCGGAAATCCCCCAAATTCACGCGAGTTTGAGAGAGCAGGTGAAATCTTTGCACTCCAAGACTCGAACGGAGCTGAATTCTGGCGGGAAAGTAGTTACTCCTAGGACAGTGGTGACCACATTGTTGTCAGTGGCGTCTGCGCTTCATCACTTGGGTGACCTTAGTTTGTCCCGTGCGAAGATGATTGTGGATGCGGTGGGGAGTGAAGATTTGAGGTCGAGTTTGGTGGCATTGTTTGAGAAGAAATGCCCAAGTGGTGAAAGCTTGATTAGTGGGTTCAAGTTGGTTTCCCATTTGGGATCTGACGCTGAAGTGAATTATGATAATTTTGCTCACGAAGTGAATGTGTTAATGGGAATTGTGTGGAAGATTGTGACTTGGGAGGCAATAACGGCATTTGTGGTGCTCGAGGGAGCTGAGTTGAATGTGAAGAAGAGTGAAGGTAGTGAAGTGAATGGAGGAGGGAATGTGAGAGTAGAGAAGAAGAGTGAGAAGAAGAAGAAGGTGGTTTTGGGTAAGGGGACTAGTGTTGTAGTACAGTTGATTAAAGATAGGTTGCGGGGCAAGGGAGGAAATGCTGTTGATAGTTTGGGGTTGTTGGTAATTTGGACGGACGAGCTTTCATCGTTTTTGGACCCCAAGGACCCGGAATTTGATGGTTTGTTGAACAAGGTTAAGGAGATAGTGGAAAGCAATGAGAGTAGAAGACTCCCAAAGCTTCCAAAGGGTACTCGTGACTTTGCAAAAGAACAAATGGTAGTAAGAAAGACAGCATTTTCGATTATTGAAGAGGTCTTTGAGAGGCACGGTGCGATGGCCCTTGACACTCCAGTTTTTGAATTGAGAGAAACCCTCATGGGAAAATATGGGGAAGATTCAAAATTGATTTATGATCTTGCTGACCAGGGAGGAGAGCTTTTATCACTGCGATATGATCTAACTGTTCCATTTGCTAGATATGTAGCTATGAATGGTATCACATCTTTCAAAAGGTACCAAATAGCAAAGGTCTATAGAAGAGACAATCCATCCAAAGGACGATATCGTGAATTTTACCAATGCGACTTTGATATTGCTGGTCAATTTGAAAAAATGGGTCCAGATTTTGAGGTTATTAAGATTTTGACAGAACTTCTTGATGAGCTGAATATTGGAGATTATGAGATTAAATTGAATCACCGAAAGTTGTTGGATGGAATGTTGGAAATCTGTGGAGTACCTCCTGAAAAATTCAGAACTATTTGTTCAAGTATTGACAAGTTAGACAAGCAGCCTTTTGAGCAGATAAAAGGAGAAATGGTGGAGGAGAAGGGCTTAAGTGTTGAGACAGCAGACAAAATTGGTGCTTTTGTAAAAGAGCGTGGACATCCTTTGGAATTGTTAAGTAAGCTTAAACAGGAGGGCAGCAAGTTCTTGGAAAGCAGTTCATCTATAGATGCGTTGAATGACTTGGAGATCTTATTTAATGCTTTGCAAAAATCAAAGTGCATAGGAAAAGTGACTTTTGACTTAAGTCTTGCAAGGGGTCTCGATTATTATACTGGAGTAATATTTGAAGCTGTTTTTAGGGGGGGTGCCCAGGTTGGTTCAATTGCTGCTGGTGGACGCTACGACAACCTTATAGGGATGTTTGGTACAAAGCAGGTTCCAGCTGTTGGTGTCAGCCTTGGAATTGAACGAGTATTTAATATAATGGAGCAGCTTAACAAAGACCAGAATCAGAGAACACGAGCAACGAAGACTGAAGTCCTAGTGAGTATATTGGGGGATGATTTAGGACGAGCAGCAGAGCTATCAACGGAGTTGTGGGACGTAAAAGTGAAAGCAGAATACTCAGTGAATAAAAGAGTGACGAAACACTTTGATCGAGCAAAGGAATCTAGGATCCCTTGGATGATAATTGAGGGTGAACGGGAGCGGAATGATAATGTTGTGAGATTGAAAAACATGGCGGCTGGCGAGGAAATCACAATTTCTAGAGATACAATTGTGGAAGAAGTTCAAAAACGGTTGAGTTTGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0032543 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0140053 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon Lyase_aromatic tRNA-synt_His

Protein Analysis

879

Amino Acids

96.2

Weight (kDa)

5.92

Isoelectric Point (pI)

34.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lyase_aromatic	PF00221	81 - 231	3.8e-09	Aromatic amino acid lyase
tRNA-synt_His	PF13393	447 - 765	2.5e-41	Histidyl-tRNA synthetase
HGTP_anticodon	PF03129	788 - 874	9.6e-12	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02760 AT3G02760
fragaria_vesca	FvH4_2g00780 FvH4_2g00780 FvH4_2g00780 FvH4_2g00780 FvH4_3g14280 FvH4_3g25240
malus_domestica	MD05G1003500.v1.1 MD10G1004600.v1.1
prunus_persica	Prupe.8G006900_v2.0.a1
pyrus_communis	pycom05g00320 pycom10g00360
rosa_chinensis	RchiOBHm_Chr6g0251201
rosa_laevigata	RLG00000015474
rosa_multiflora	Rmu_sc0004077.1_g000009
rosa_roxburghii	Rroxscaffold_7G00216930
rosa_rugosa	Rorug05G0500700.1
rosa_samantha	Rh6AG010100 Rh6BG001900 Rh6CG007000 Rh6DG007500
rosa_wichuraiana	Rw6G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	640
Acc16I	TGCGCA	1 cut(s)	95
Acc36I	ACCTGC	2 cut(s)	640, 2245
Acc65I	GGTACC	1 cut(s)	1605
AccB1I	GGYRCC	2 cut(s)	1605, 2186
AccB7I	CCANNNNNTGG	1 cut(s)	2267
AccBSI	CCGCTC	1 cut(s)	2530
AccI	GTMKAC	1 cut(s)	431
AccII	CGCG	3 cut(s)	278, 425, 635
AclWI	GGATC	3 cut(s)	919, 2491, 2504
AcoI	YGGCCR	1 cut(s)	546
AcsI	RAATTY	6 cut(s)	365, 627, 688, 1262, 1661, 1841
AcuI	CTGAAG	2 cut(s)	942, 2376
AcyI	GRCGYC	1 cut(s)	746
AdeI	CACNNNGTG	1 cut(s)	952
AfaI	GTAC	8 cut(s)	268, 282, 521, 1149, 1342, 1607, 1830, 2248
AfiI	CCNNNNNNNGG	5 cut(s)	76, 313, 486, 1646, 2267
AflII	CTTAAG	2 cut(s)	1927, 2122
AhdI	GACNNNNNGTC	1 cut(s)	415
AhlI	ACTAGT	1 cut(s)	1136
AjnI	CCWGG	2 cut(s)	1515, 2190
AjuI	GAANNNNNNNTTGG	2 cut(s)	1106, 1138
AleI	CACNNNNGTG	1 cut(s)	765
Alw21I	GWGCWC	2 cut(s)	321, 1020
Alw26I	GTCTC	7 cut(s)	48, 172, 290, 303, 1626, 1931, 2144
AlwI	GGATC	3 cut(s)	919, 2491, 2504
Ama87I	CYCGRG	3 cut(s)	306, 320, 1019
AoxI	GGCC	4 cut(s)	79, 477, 546, 1428
ApeKI	GCWGC	9 cut(s)	10, 452, 524, 565, 1882, 2016, 2207, 2311, 2396
ApoI	RAATTY	6 cut(s)	365, 627, 688, 1262, 1661, 1841
ArsI	GACNNNNNNTTYG	4 cut(s)	1247, 1279, 1372, 1404
Asp700I	GAANNNNTTC	1 cut(s)	2610
Asp718I	GGTACC	1 cut(s)	1605
AspA2I	CCTAGG	1 cut(s)	713
AspLEI	GCGC	4 cut(s)	96, 112, 403, 754
AspS9I	GGNCC	6 cut(s)	79, 409, 1246, 1255, 1429, 1709
AsuC2I	CCSGG	1 cut(s)	1259
AsuHPI	GGTGA	7 cut(s)	547, 664, 736, 779, 881, 1781, 2531
AvaI	CYCGRG	3 cut(s)	306, 320, 1019
AvaII	GGWCC	4 cut(s)	409, 1246, 1255, 1709
AvrII	CCTAGG	1 cut(s)	713
BaeGI	GKGCMC	1 cut(s)	2191
BaeI	ACNNNNGTAYC	2 cut(s)	2586, 2619
BalI	TGGCCA	1 cut(s)	548
BamHI	GGATCC	1 cut(s)	2496
BanI	GGYRCC	2 cut(s)	1605, 2186
BanII	GRGCYC	2 cut(s)	41, 321
BauI	CACGAG	2 cut(s)	1344, 2340
BbsI	GAAGAC	2 cut(s)	1324, 2357
Bbv12I	GWGCWC	2 cut(s)	321, 1020
BbvCI	CCTCAGC	1 cut(s)	379
BbvI	GCAGC	8 cut(s)	464, 536, 552, 1894, 2028, 2194, 2323, 2408
BccI	CCATC	7 cut(s)	305, 496, 509, 1262, 1420, 1648, 1799
BceAI	ACGGC	1 cut(s)	1020
BciT130I	CCWGG	2 cut(s)	1517, 2192
BcnI	CCSGG	1 cut(s)	1259
BcoDI	GTCTC	7 cut(s)	48, 172, 290, 303, 1626, 1931, 2144
BcuI	ACTAGT	1 cut(s)	1136
BfaI	CTAG	6 cut(s)	714, 1137, 1566, 2363, 2493, 2589
BfmI	CTRYAG	2 cut(s)	1624, 2043
BfoI	RGCGCY	1 cut(s)	113
BfrI	CTTAAG	2 cut(s)	1927, 2122
BfuAI	ACCTGC	2 cut(s)	640, 2245
BglII	AGATCT	1 cut(s)	2067
BlnI	CCTAGG	1 cut(s)	713
Bme1390I	CCNGG	3 cut(s)	1259, 1517, 2192
Bme18I	GGWCC	4 cut(s)	409, 1246, 1255, 1709
BmeRI	GACNNNNNGTC	1 cut(s)	415
BmeT110I	CYCGRG	3 cut(s)	306, 320, 1019
BmgT120I	GGNCC	6 cut(s)	79, 409, 1246, 1255, 1429, 1709
BmiI	GGNNCC	8 cut(s)	253, 1248, 1257, 1607, 1710, 2188, 2259, 2498
BmrFI	CCNGG	3 cut(s)	1259, 1517, 2192
BmsI	GCATC	3 cut(s)	433, 793, 2038
BoxI	GACNNNNGTC	2 cut(s)	2124, 2357
BpiI	GAAGAC	2 cut(s)	1324, 2357
BplI	GAGNNNNNCTC	4 cut(s)	198, 230, 1449, 1481
BpmI	CTGGAG	2 cut(s)	1425, 2175
Bpu10I	CCTNAGC	1 cut(s)	379
BpuMI	CCSGG	1 cut(s)	1259
BsaHI	GRCGYC	1 cut(s)	746
BsaI	GGTCTC	1 cut(s)	2144
BsaJI	CCNNGG	7 cut(s)	75, 713, 1251, 1516, 2190, 2278, 2501
BsaWI	WCCGGW	1 cut(s)	248
BsaXI	ACNNNNNCTCC	8 cut(s)	116, 146, 308, 338, 1065, 1095, 1896, 1926
Bsc4I	CCNNNNNNNGG	5 cut(s)	76, 313, 486, 1646, 2267
Bse1I	ACTGG	4 cut(s)	14, 415, 1442, 2158
Bse3DI	GCAATG	1 cut(s)	1312
BseBI	CCWGG	2 cut(s)	1517, 2192
BseDI	CCNNGG	7 cut(s)	75, 713, 1251, 1516, 2190, 2278, 2501
BseGI	GGATG	8 cut(s)	520, 808, 1640, 1810, 1978, 2244, 2386, 2511
BseLI	CCNNNNNNNGG	5 cut(s)	76, 313, 486, 1646, 2267
BseMI	GCAATG	1 cut(s)	1312
BseMII	CTCAG	3 cut(s)	393, 1020, 2460
BseNI	ACTGG	4 cut(s)	14, 415, 1442, 2158
BseSI	GKGCMC	1 cut(s)	2191
BseXI	GCAGC	8 cut(s)	464, 536, 552, 1894, 2028, 2194, 2323, 2408
Bsh1236I	CGCG	3 cut(s)	278, 425, 635
BshFI	GGCC	4 cut(s)	81, 479, 548, 1430
BshNI	GGYRCC	2 cut(s)	1605, 2186
BsiHKAI	GWGCWC	2 cut(s)	321, 1020
BsiHKCI	CYCGRG	3 cut(s)	306, 320, 1019
BsiSI	CCGG	2 cut(s)	249, 1259
BsiWI	CGTACG	1 cut(s)	266
BslFI	GGGAC	3 cut(s)	766, 1147, 2435
BslI	CCNNNNNNNGG	5 cut(s)	76, 313, 486, 1646, 2267
BsmAI	GTCTC	7 cut(s)	48, 172, 290, 303, 1626, 1931, 2144
BsmBI	CGTCTC	3 cut(s)	48, 172, 290
BsmFI	GGGAC	3 cut(s)	766, 1147, 2435
BsmI	GAATGC	1 cut(s)	206
BsnI	GGCC	4 cut(s)	81, 479, 548, 1430
Bso31I	GGTCTC	1 cut(s)	2144
BsoBI	CYCGRG	3 cut(s)	306, 320, 1019
Bsp1286I	GDGCHC	4 cut(s)	41, 321, 1020, 2191
Bsp143I	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
BspANI	GGCC	4 cut(s)	81, 479, 548, 1430
BspCNI	CTCAG	3 cut(s)	392, 1021, 2459
BspFNI	CGCG	3 cut(s)	278, 425, 635
BspLI	GGNNCC	8 cut(s)	253, 1248, 1257, 1607, 1710, 2188, 2259, 2498
BspMI	ACCTGC	2 cut(s)	640, 2245
BspPI	GGATC	3 cut(s)	919, 2491, 2504
BspT107I	GGYRCC	2 cut(s)	1605, 2186
BspTI	CTTAAG	2 cut(s)	1927, 2122
BspTNI	GGTCTC	1 cut(s)	2144
BsrBI	CCGCTC	1 cut(s)	2530
BsrDI	GCAATG	1 cut(s)	1312
BsrI	ACTGG	4 cut(s)	14, 415, 1442, 2158
BssECI	CCNNGG	7 cut(s)	75, 713, 1251, 1516, 2190, 2278, 2501
BssMI	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
BssNI	GRCGYC	1 cut(s)	746
BssSI	CACGAG	2 cut(s)	1344, 2340
BssT1I	CCWWGG	5 cut(s)	75, 713, 1251, 2278, 2501
Bst2BI	CACGAG	2 cut(s)	1344, 2340
Bst2UI	CCWGG	2 cut(s)	1517, 2192
Bst4CI	ACNGT	8 cut(s)	193, 271, 436, 721, 1152, 1421, 1555, 2622
Bst6I	CTCTTC	5 cut(s)	336, 1040, 1091, 1395, 1624
BstACI	GRCGYC	1 cut(s)	746
BstAFI	CTTAAG	2 cut(s)	1927, 2122
BstC8I	GCNNGC	2 cut(s)	233, 2569
BstDEI	CTNAG	4 cut(s)	379, 773, 1029, 2446
BstEII	GGTNACC	2 cut(s)	724, 767
BstENI	CCTNNNNNAGG	1 cut(s)	74
BstF5I	GGATG	8 cut(s)	520, 808, 1640, 1810, 1978, 2244, 2386, 2511
BstFNI	CGCG	3 cut(s)	278, 425, 635
BstH2I	RGCGCY	1 cut(s)	113
BstHHI	GCGC	4 cut(s)	96, 112, 403, 754
BstKTI	GATC	6 cut(s)	914, 1507, 1549, 2070, 2479, 2499
BstMAI	GTCTC	7 cut(s)	48, 172, 290, 303, 1626, 1931, 2144
BstMBI	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
BstMWI	GCNNNNNNNGC	6 cut(s)	118, 325, 452, 476, 1004, 1891
BstNI	CCWGG	2 cut(s)	1517, 2192
BstPAI	GACNNNNGTC	2 cut(s)	2124, 2357
BstPI	GGTNACC	2 cut(s)	724, 767
BstSCI	CCNGG	3 cut(s)	1257, 1515, 2190
BstSFI	CTRYAG	2 cut(s)	1624, 2043
BstSLI	GKGCMC	1 cut(s)	2191
BstUI	CGCG	3 cut(s)	278, 425, 635
BstV1I	GCAGC	8 cut(s)	464, 536, 552, 1894, 2028, 2194, 2323, 2408
BstV2I	GAAGAC	2 cut(s)	1324, 2357
BstX2I	RGATCY	3 cut(s)	911, 2067, 2496
BstYI	RGATCY	3 cut(s)	911, 2067, 2496
BsuRI	GGCC	4 cut(s)	81, 479, 548, 1430
BtgZI	GCGATG	3 cut(s)	129, 470, 1439
BtsCI	GGATG	8 cut(s)	520, 808, 1640, 1810, 1978, 2244, 2386, 2511
BtsI	GCAGTG	1 cut(s)	1535
BtsIMutI	CAGTG	5 cut(s)	21, 726, 747, 1535, 2454
BveI	ACCTGC	2 cut(s)	640, 2245
Cac8I	GCNNGC	2 cut(s)	233, 2569
CfoI	GCGC	4 cut(s)	96, 112, 403, 754
Cfr13I	GGNCC	6 cut(s)	79, 409, 1246, 1255, 1429, 1709
CseI	GACGC	3 cut(s)	735, 926, 2226
Csp6I	GTAC	8 cut(s)	267, 281, 520, 1148, 1341, 1606, 1829, 2247
CviAII	CATG	2 cut(s)	1468, 2560
CviQI	GTAC	8 cut(s)	267, 281, 520, 1148, 1341, 1606, 1829, 2247
DdeI	CTNAG	4 cut(s)	379, 773, 1029, 2446
DpnI	GATC	6 cut(s)	913, 1506, 1548, 2069, 2478, 2498
DpnII	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
DraIII	CACNNNGTG	1 cut(s)	952
DriI	GACNNNNNGTC	1 cut(s)	415
EaeI	YGGCCR	1 cut(s)	546
Eam1104I	CTCTTC	5 cut(s)	336, 1040, 1091, 1395, 1624
Eam1105I	GACNNNNNGTC	1 cut(s)	415
EarI	CTCTTC	5 cut(s)	336, 1040, 1091, 1395, 1624
EciI	GGCGGA	2 cut(s)	20, 409
Ecl136II	GAGCTC	1 cut(s)	319
Eco130I	CCWWGG	5 cut(s)	75, 713, 1251, 2278, 2501
Eco24I	GRGCYC	2 cut(s)	41, 321
Eco31I	GGTCTC	1 cut(s)	2144
Eco32I	GATATC	1 cut(s)	1655
Eco47I	GGWCC	4 cut(s)	409, 1246, 1255, 1709
Eco53kI	GAGCTC	1 cut(s)	319
Eco57I	CTGAAG	2 cut(s)	942, 2376
Eco88I	CYCGRG	3 cut(s)	306, 320, 1019
Eco91I	GGTNACC	2 cut(s)	724, 767
EcoICRI	GAGCTC	1 cut(s)	319
EcoNI	CCTNNNNNAGG	1 cut(s)	74
EcoO109I	RGGNCCY	2 cut(s)	79, 1255
EcoO65I	GGTNACC	2 cut(s)	724, 767
EcoRI	GAATTC	2 cut(s)	365, 688
EcoRII	CCWGG	2 cut(s)	1515, 2190
EcoRV	GATATC	1 cut(s)	1655
EcoT14I	CCWWGG	5 cut(s)	75, 713, 1251, 2278, 2501
EcoT38I	GRGCYC	2 cut(s)	41, 321
ErhI	CCWWGG	5 cut(s)	75, 713, 1251, 2278, 2501
Esp3I	CGTCTC	3 cut(s)	48, 172, 290
FaeI	CATG	2 cut(s)	1471, 2563
FaqI	GGGAC	3 cut(s)	766, 1147, 2435
FatI	CATG	2 cut(s)	1467, 2559
FauI	CCCGC	3 cut(s)	383, 689, 1164
FblI	GTMKAC	1 cut(s)	431
FokI	GGATG	8 cut(s)	527, 815, 1627, 1817, 1965, 2251, 2393, 2518
FriOI	GRGCYC	2 cut(s)	41, 321
FspBI	CTAG	6 cut(s)	714, 1137, 1566, 2363, 2493, 2589
FspI	TGCGCA	1 cut(s)	95
GlaI	GCGC	4 cut(s)	95, 111, 402, 753
GsuI	CTGGAG	2 cut(s)	1425, 2175
HaeII	RGCGCY	1 cut(s)	113
HaeIII	GGCC	4 cut(s)	81, 479, 548, 1430
HapII	CCGG	2 cut(s)	249, 1259
HgaI	GACGC	3 cut(s)	735, 926, 2226
HhaI	GCGC	4 cut(s)	96, 112, 403, 754
Hin1I	GRCGYC	1 cut(s)	746
Hin1II	CATG	2 cut(s)	1471, 2563
Hin6I	GCGC	4 cut(s)	94, 110, 401, 752
HinP1I	GCGC	4 cut(s)	94, 110, 401, 752
HindIII	AAGCTT	5 cut(s)	233, 593, 874, 1328, 2000
HinfI	GANTC	7 cut(s)	125, 673, 1320, 1487, 1786, 2329, 2489
HpaII	CCGG	2 cut(s)	249, 1259
HphI	GGTGA	7 cut(s)	547, 664, 736, 779, 881, 1781, 2531
Hpy166II	GTNNAC	4 cut(s)	432, 1976, 2216, 2522
Hpy188I	TCNGA	5 cut(s)	442, 616, 916, 1847, 2334
Hpy8I	GTNNAC	4 cut(s)	432, 1976, 2216, 2522
Hpy99I	CGWCG	1 cut(s)	221
HpyAV	CCTTC	6 cut(s)	469, 523, 553, 1046, 1108, 1915
HpyCH4III	ACNGT	8 cut(s)	193, 271, 436, 721, 1152, 1421, 1555, 2622
HpyCH4IV	ACGT	3 cut(s)	261, 283, 2424
HpyCH4V	TGCA	7 cut(s)	204, 231, 664, 1355, 2086, 2100, 2132
HpyF10VI	GCNNNNNNNGC	6 cut(s)	118, 325, 452, 476, 1004, 1891
HpyF3I	CTNAG	4 cut(s)	379, 773, 1029, 2446
HpySE526I	ACGT	3 cut(s)	261, 283, 2424
Hsp92I	GRCGYC	1 cut(s)	746
Hsp92II	CATG	2 cut(s)	1471, 2563
HspAI	GCGC	4 cut(s)	94, 110, 401, 752
KpnI	GGTACC	1 cut(s)	1609
Kzo9I	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
LmnI	GCTCC	7 cut(s)	7, 71, 316, 682, 1025, 2308, 2527
Lsp1109I	GCAGC	8 cut(s)	464, 536, 552, 1894, 2028, 2194, 2323, 2408
LweI	GCATC	3 cut(s)	433, 793, 2038
MaeI	CTAG	6 cut(s)	714, 1137, 1566, 2363, 2493, 2589
MaeII	ACGT	3 cut(s)	261, 283, 2424
MaeIII	GTNAC	7 cut(s)	706, 724, 767, 985, 1346, 2110, 2461
MalI	GATC	6 cut(s)	913, 1506, 1548, 2069, 2478, 2498
MbiI	CCGCTC	1 cut(s)	2530
MboI	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
MfeI	CAATTG	2 cut(s)	2202, 2598
MflI	RGATCY	3 cut(s)	911, 2067, 2496
MhlI	GDGCHC	4 cut(s)	41, 321, 1020, 2191
MlsI	TGGCCA	1 cut(s)	548
MluNI	TGGCCA	1 cut(s)	548
MlyI	GAGTC	3 cut(s)	119, 667, 1314
MmeI	TCCRAC	3 cut(s)	339, 1782, 1794
Mox20I	TGGCCA	1 cut(s)	548
MroXI	GAANNNNTTC	1 cut(s)	2610
MscI	TGGCCA	1 cut(s)	548
MslI	CAYNNNNRTG	1 cut(s)	765
Msp20I	TGGCCA	1 cut(s)	548
MspA1I	CMGCKG	1 cut(s)	2264
MspCI	CTTAAG	2 cut(s)	1927, 2122
MspI	CCGG	2 cut(s)	249, 1259
MspR9I	CCNGG	3 cut(s)	1259, 1517, 2192
MunI	CAATTG	2 cut(s)	2202, 2598
Mva1269I	GAATGC	1 cut(s)	206
MvaI	CCWGG	2 cut(s)	1517, 2192
MvnI	CGCG	3 cut(s)	278, 425, 635
MwoI	GCNNNNNNNGC	6 cut(s)	118, 325, 452, 476, 1004, 1891
NciI	CCSGG	1 cut(s)	1259
NdeII	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
NlaIII	CATG	2 cut(s)	1471, 2563
NlaIV	GGNNCC	8 cut(s)	253, 1248, 1257, 1607, 1710, 2188, 2259, 2498
NmuCI	GTSAC	6 cut(s)	724, 767, 985, 1346, 2110, 2461
NsbI	TGCGCA	1 cut(s)	95
OliI	CACNNNNGTG	1 cut(s)	765
PaeR7I	CTCGAG	1 cut(s)	1019
PaqCI	CACCTGC	1 cut(s)	640
PcsI	WCGNNNNNNNCGW	2 cut(s)	327, 336
PctI	GAATGC	1 cut(s)	206
PdmI	GAANNNNTTC	1 cut(s)	2610
PfeI	GAWTC	4 cut(s)	1487, 1786, 2329, 2489
Pfl23II	CGTACG	1 cut(s)	266
PflMI	CCANNNNNTGG	1 cut(s)	2267
PleI	GAGTC	3 cut(s)	119, 667, 1314
PpsI	GAGTC	3 cut(s)	119, 667, 1314
PpuMI	RGGWCCY	1 cut(s)	1255
PshAI	GACNNNNGTC	2 cut(s)	2124, 2357
Psp124BI	GAGCTC	1 cut(s)	321
Psp5II	RGGWCCY	1 cut(s)	1255
Psp6I	CCWGG	2 cut(s)	1515, 2190
PspEI	GGTNACC	2 cut(s)	724, 767
PspGI	CCWGG	2 cut(s)	1515, 2190
PspLI	CGTACG	1 cut(s)	266
PspN4I	GGNNCC	8 cut(s)	253, 1248, 1257, 1607, 1710, 2188, 2259, 2498
PspPI	GGNCC	6 cut(s)	79, 409, 1246, 1255, 1429, 1709
PspPPI	RGGWCCY	1 cut(s)	1255
PspXI	VCTCGAGB	1 cut(s)	1019
PsuI	RGATCY	3 cut(s)	911, 2067, 2496
PvuII	CAGCTG	1 cut(s)	2264
RsaI	GTAC	8 cut(s)	268, 282, 521, 1149, 1342, 1607, 1830, 2248
RsaNI	GTAC	8 cut(s)	267, 281, 520, 1148, 1341, 1606, 1829, 2247
RseI	CAYNNNNRTG	1 cut(s)	765
SacI	GAGCTC	1 cut(s)	321
Sau3AI	GATC	6 cut(s)	911, 1504, 1546, 2067, 2476, 2496
Sau96I	GGNCC	6 cut(s)	79, 409, 1246, 1255, 1429, 1709
SchI	GAGTC	3 cut(s)	119, 667, 1314
ScrFI	CCNGG	3 cut(s)	1259, 1517, 2192
SduI	GDGCHC	4 cut(s)	41, 321, 1020, 2191
SfaNI	GCATC	3 cut(s)	433, 793, 2038
SfcI	CTRYAG	2 cut(s)	1624, 2043
Sfr274I	CTCGAG	1 cut(s)	1019
SinI	GGWCC	4 cut(s)	409, 1246, 1255, 1709
SlaI	CTCGAG	1 cut(s)	1019
SmiMI	CAYNNNNRTG	1 cut(s)	765
SmlI	CTYRAG	3 cut(s)	1019, 1927, 2122
SmoI	CTYRAG	3 cut(s)	1019, 1927, 2122
SpeI	ACTAGT	1 cut(s)	1136
SspI	AATATT	2 cut(s)	1762, 2163
SspMI	CTAG	6 cut(s)	714, 1137, 1566, 2363, 2493, 2589
SstI	GAGCTC	1 cut(s)	321
StyD4I	CCNGG	3 cut(s)	1257, 1515, 2190
StyI	CCWWGG	5 cut(s)	75, 713, 1251, 2278, 2501
TaaI	ACNGT	8 cut(s)	193, 271, 436, 721, 1152, 1421, 1555, 2622
TaiI	ACGT	3 cut(s)	264, 286, 2427
TaqI	TCGA	9 cut(s)	123, 195, 500, 676, 830, 1020, 1391, 2142, 2479
TaqII	GACCGA	1 cut(s)	353
TatI	WGTACW	1 cut(s)	1147
TauI	GCSGC	2 cut(s)	425, 2567
TfiI	GAWTC	4 cut(s)	1487, 1786, 2329, 2489
TscAI	CASTG	5 cut(s)	21, 726, 747, 1542, 2454
TseFI	GTSAC	6 cut(s)	724, 767, 985, 1346, 2110, 2461
TseI	GCWGC	9 cut(s)	10, 452, 524, 565, 1882, 2016, 2207, 2311, 2396
Tsp45I	GTSAC	6 cut(s)	724, 767, 985, 1346, 2110, 2461
TspDTI	ATGAA	4 cut(s)	746, 1224, 1595, 2028
TspGWI	ACGGA	5 cut(s)	49, 254, 695, 1238, 2426
TspRI	CASTG	5 cut(s)	21, 726, 747, 1542, 2454
Van91I	CCANNNNNTGG	1 cut(s)	2267
Vha464I	CTTAAG	2 cut(s)	1927, 2122
VpaK11BI	GGWCC	4 cut(s)	409, 1246, 1255, 1709
XagI	CCTNNNNNAGG	1 cut(s)	74
XapI	RAATTY	6 cut(s)	365, 627, 688, 1262, 1661, 1841
XbaI	TCTAGA	1 cut(s)	2588
XhoI	CTCGAG	1 cut(s)	1019
XmaJI	CCTAGG	1 cut(s)	713
XmiI	GTMKAC	1 cut(s)	431
XmnI	GAANNNNTTC	1 cut(s)	2610
XspI	CTAG	6 cut(s)	714, 1137, 1566, 2363, 2493, 2589

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.