Rw6G000990

Histidine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008801
Physical Location & Seq
Reverse (-)
1410495 .. 1416422
5928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000990.1

Sequence Viewer

Length: 2643 bp
ATGGCGGGCCTTGCAGTGATAAGAGTCGGAGGCAAAGGCTCATCTCTCTCCTCCTCCGCCGTCTACGCCGTCGCCAATGCCCTCGCTCACGTCACTATGGACTCTTCCGCCCTGGACAGACTCTCCTCCTCCTCCTCCGCCGCCAATCTCAAGCCTTACCACCTCGTCTCTCTCCTCCCTTCCTTCCCGTCCGTCCAAGAATCCAGAGCCTCCCTCGTTGTCCTCCTCGCCAAACTCCTCTCCCTCAACATCCGTACGGTTCTCCCGGTTCGAATCGCCGCCGCCCTAAATTCCGGCTCCACTCAAGACCTGGGGCTCGAAACCCTCGATTTGACCGAAGAGGAGGCTCTTGCACTGGAGAAGCTCAAAGATTCGTCTGCTTTGTACGGCGTCTGTGCGCTTCTGGACCACCAATCGGCGGCTCTGTCCACGGTTTCGGACGCCGTCGCGGCGCTTTCTTGCGAGGCGTTGAAGGCCGACGTGGCGGGGTTTAATTCGGTTGACGCCGGGGACGGGTACGCCGCGAAGGAGGTGATCGGAGTTGCTAGTGATTTGAAGGTTTTGTTGAACGGGTCGAAGTTAGTCGGCAAAGTTGAGACTGAAGCTGTATCGGAGATTCCGAAAATTCACGCCAGTCTGAGAGTGCAAGTGAAATTGGTGCATGCGAAGACTAGGGAGGAGCTGAATTCTGGAGCCAAAGTAGGGAAAGGTGGTTCCCGGAGCGCCAGGTCGGAGAGGACGGCATTGTTGGCGTTGGCGGCGGCGCTGTTTGACTTGGGGGATTACAGTCTCCGGCGAGCGAAGTTGGATTTGGAGGCAGTGGGGAGTGAGAGTCTGCGTTTGAGATTGGTGGAGTTGTTTGGGAAGCAATGCCCTAGTGGTGAAAGCTTGAGAAGTGGGCTTAAGCTGGTTCAGGAGTTGGCTTTCGAAGAGGAAGAGAATTATGTTAAGTTTGCGCATGCAGTGAATGTGTTGATGGAGCTTGTTTGGAAGATTGTGACTTGGGAGGCCATAACAGTGTTTGTGGCGCTGGAGGGAGCAGAGTTGAGTGGAAAGGGTCAAGGAGGTGAAGTGAATGGAGAAGTGAATGCGAAAGCAGGGAAGAAGAGTGAGAAGAAGAAGAAGGCGGTTTTGGGGAAAGGGACAACTGTTATGGTACAGTTGATTAAAGATAAGCTGCAGGGTGAGGGAGGAAATGCTGTTGATAGTTCAGAGTTGTTGGAAAATTGGGTCAAGGAGCTTTTATTGTTTTTGGACCCGAAGAGCTCTGAGTTTGATGGTTTGTTGAACAAGGTGAAGGAGATAGTAGAAAGCAATGAAACTAGAAGACTCCCAAAGCTTCCAAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATAAGAAAGAAAGCATTTTCGATAATAGAAGATGTTTTTGAGAGGCATGGTGCCACAGCTCTGGATACTCCAGTTTTTGAATTGAGAGACACTCTCATGGGCAAATATGGGGAAGACTCGAAATTGATTTATGATCTTGCTGACCAGGGAGGAGAGCTTTCCTCACTGCGATATGATTTAACTGTTCCATTTGCTAGGTATGTTGCCATGAATGGTATCACATCCTTTAAAAGGTACCAAATAGCAAAGGTCTACAGAAGAGACAATCCCTCTAAGGGTAGATACCGTGAGTTCTACCAATGCGACTTTGATATTGCTGGCCCAAGTGAAAAAATGGGACCAGATTTTGAGGCTGTCTATGTGCTAACAGAACTTCTTGATGAGCTGAATATTGGAGACTACGAGGTTAAGTTAAATCACCGAAAGTTGTTGGATGGAATGTTGGAAATATGTGGAGTGCCTCCTGAAAAATTCAGAACTATTTGTTCTAGTATCGACAAGTTAGACAAGCAGTCATTCGAGCAAATAAAAAAAGAAATGGTGGAGGAGAAGGGCTTAAGTGTTGAGACAGCCGACAGAATTGGTACTTTTGTGAAAGAGCGGGGACATCCTTTGGAATTGTTATCTAAGCTTAAACAGGAGGGCAGCGAGTTCTTGGAGAACAATTCATCTATAGATGCACTGAATGACCTGGAGATCTTGTTCAAATGTTTGGAAAAGTCACAGTGTATTGGAAAAGTGGTTTTTGACTTGAGTCTTGCAAGGGGTCTTGATTATTATACTGGAGTCATATATGAAGCTGTTTTCAAGGGTGGTGCGCAGATGGGTTCCATTGCTGCTGGTGGACGGTATGACAACCTTATTGGGATGTTTGGTACAAAGCAAGTTCCAGCAGTTGGTGTCAGCCTTGGAATTGAGCGAGTATTTGCTATAATGGAGCAGCTTCACAAAGACCAGAATCAGACAACCCGGGCAACCAAGACTGAGGTCCTAGTGAGTATATTAGGGGATGATATAGGACTAGCAGCAGAGCTATCAGGTGAGTTGTGGAAGGGAAAAGTGCAAGCAGAATTCTTGGTCAATAAGAGAGTGACGAAGCACTTTGATCGAGCAAAGGATTCTAAGATTCCCTGGATGATAATTGTGGGTGAAAAAGAGCAGAAAGAAGGGGTTGTTCGATTGAAGAACATTGCAGCTGGGGAAGAAGTTGTAATTCAGAGAAGTGATATTGTTGAAGAACTTAAGAAGCGGTTGAATGCATGA

Protein Analysis

880

Amino Acids

96.05

Weight (kDa)

5.92

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_aromatic PF00221 102 - 230 1.3e-06 Aromatic amino acid lyase
tRNA-synt_His PF13393 450 - 768 3.8e-39 Histidyl-tRNA synthetase
HGTP_anticodon2 PF12745 786 - 878 1.1e-06 Anticodon binding domain of tRNAs
HGTP_anticodon PF03129 800 - 877 4.7e-13 Anticodon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 957, 2199
Acc65I GGTACC 1 cut(s) 1614
AccB1I GGYRCC 2 cut(s) 1430, 1614
AccB7I CCANNNNNTGG 1 cut(s) 2276
AccBSI CCGCTC 1 cut(s) 1981
AccI GTMKAC 2 cut(s) 63, 1632
AccII CGCG 2 cut(s) 449, 524
AcsI RAATTY 5 cut(s) 289, 624, 685, 1850, 2450
AcuI CTGAAG 1 cut(s) 621
AcyI GRCGYC 3 cut(s) 390, 441, 504
AfaI GTAC 8 cut(s) 256, 386, 518, 1158, 1351, 1616, 1966, 2257
AfiI CCNNNNNNNGG 7 cut(s) 415, 418, 513, 702, 1611, 1655, 2276
AflII CTTAAG 3 cut(s) 902, 1936, 2621
AhdI GACNNNNNGTC 1 cut(s) 1891
AjiI CACGTC 2 cut(s) 91, 481
AjnI CCWGG 6 cut(s) 111, 309, 725, 1524, 2070, 2510
AjuI GAANNNNNNNTTGG 4 cut(s) 794, 826, 1115, 1147
AloI GAACNNNNNNTCC 4 cut(s) 697, 729, 2066, 2098
Alw21I GWGCWC 1 cut(s) 1268
Alw26I GTCTC 7 cut(s) 172, 590, 794, 1461, 1635, 1770, 1940
Ama87I CYCGRG 1 cut(s) 2349
AoxI GGCC 4 cut(s) 7, 474, 1008, 1699
ApeKI GCWGC 6 cut(s) 1177, 2025, 2216, 2320, 2405, 2573
ApoI RAATTY 5 cut(s) 289, 624, 685, 1850, 2450
Asp718I GGTACC 1 cut(s) 1614
AspLEI GCGC 7 cut(s) 400, 454, 725, 766, 958, 1030, 2200
AspS9I GGNCC 6 cut(s) 7, 406, 1255, 1700, 1718, 2368
AsuC2I CCSGG 5 cut(s) 266, 508, 718, 2350, 2351
AsuHPI GGTGA 8 cut(s) 544, 893, 1079, 1196, 1306, 1790, 2432, 2540
AsuII TTCGAA 2 cut(s) 271, 927
AvaI CYCGRG 1 cut(s) 2349
AvaII GGWCC 4 cut(s) 406, 1255, 1718, 2368
BaeI ACNNNNGTAYC 4 cut(s) 1437, 1470, 1956, 1989
BanI GGYRCC 2 cut(s) 1430, 1614
BanII GRGCYC 2 cut(s) 318, 1268
BauI CACGAG 1 cut(s) 1353
BbsI GAAGAC 3 cut(s) 674, 1333, 1500
Bbv12I GWGCWC 1 cut(s) 1268
BbvI GCAGC 6 cut(s) 1164, 2037, 2203, 2332, 2417, 2585
BccI CCATC 4 cut(s) 970, 1271, 1808, 2197
BceAI ACGGC 5 cut(s) 44, 53, 403, 428, 756
BcgI CGANNNNNNTGC 2 cut(s) 2468, 2502
BciT130I CCWGG 6 cut(s) 113, 311, 727, 1526, 2072, 2512
BciVI GTATCC 1 cut(s) 1438
BcnI CCSGG 5 cut(s) 266, 508, 718, 2350, 2351
BcoDI GTCTC 7 cut(s) 172, 590, 794, 1461, 1635, 1770, 1940
BfaI CTAG 8 cut(s) 546, 672, 876, 1323, 1575, 1869, 2372, 2402
BfmI CTRYAG 3 cut(s) 1178, 1633, 2052
BfoI RGCGCY 4 cut(s) 455, 726, 767, 1031
BfrI CTTAAG 3 cut(s) 902, 1936, 2621
BfuI GTATCC 1 cut(s) 1438
BglI GCCNNNNNGGC 2 cut(s) 449, 482
BglII AGATCT 1 cut(s) 2076
Bme18I GGWCC 4 cut(s) 406, 1255, 1718, 2368
BmeRI GACNNNNNGTC 1 cut(s) 1891
BmeT110I CYCGRG 1 cut(s) 2349
BmgBI CACGTC 2 cut(s) 91, 481
BmgT120I GGNCC 6 cut(s) 7, 406, 1255, 1700, 1718, 2368
BmiI GGNNCC 8 cut(s) 298, 694, 715, 1257, 1432, 1616, 1719, 2209
BmsI GCATC 1 cut(s) 2047
BoxI GACNNNNGTC 2 cut(s) 2133, 2366
BpiI GAAGAC 3 cut(s) 674, 1333, 1500
BplI GAGNNNNNCTC 8 cut(s) 198, 230, 1456, 1488, 1458, 1490, 1526, 1558
BpmI CTGGAG 6 cut(s) 377, 711, 1052, 1434, 2093, 2184
Bpu14I TTCGAA 2 cut(s) 271, 927
BpuEI CTTGAG 4 cut(s) 134, 288, 910, 2152
BpuMI CCSGG 5 cut(s) 266, 508, 718, 2350, 2351
BsaHI GRCGYC 3 cut(s) 390, 441, 504
BsaJI CCNNGG 8 cut(s) 111, 310, 429, 507, 1525, 2287, 2349, 2510
Bsc4I CCNNNNNNNGG 7 cut(s) 415, 418, 513, 702, 1611, 1655, 2276
Bse1I ACTGG 4 cut(s) 360, 633, 1451, 2167
Bse3DI GCAATG 4 cut(s) 875, 1321, 2211, 2568
BseBI CCWGG 6 cut(s) 113, 311, 727, 1526, 2072, 2512
BseDI CCNNGG 8 cut(s) 111, 310, 429, 507, 1525, 2287, 2349, 2510
BseGI GGATG 7 cut(s) 249, 1601, 1819, 1987, 2253, 2395, 2520
BseLI CCNNNNNNNGG 7 cut(s) 415, 418, 513, 702, 1611, 1655, 2276
BseMI GCAATG 4 cut(s) 875, 1321, 2211, 2568
BseMII CTCAG 3 cut(s) 629, 1260, 2355
BseNI ACTGG 4 cut(s) 360, 633, 1451, 2167
BseXI GCAGC 6 cut(s) 1164, 2037, 2203, 2332, 2417, 2585
BseYI CCCAGC 1 cut(s) 2576
Bsh1236I CGCG 2 cut(s) 449, 524
BshFI GGCC 4 cut(s) 9, 476, 1010, 1701
BshNI GGYRCC 2 cut(s) 1430, 1614
BsiHKAI GWGCWC 1 cut(s) 1268
BsiHKCI CYCGRG 1 cut(s) 2349
BsiSI CCGG 6 cut(s) 266, 294, 507, 718, 793, 2350
BsiWI CGTACG 1 cut(s) 254
BslFI GGGAC 4 cut(s) 524, 1156, 1731, 1998
BslI CCNNNNNNNGG 7 cut(s) 415, 418, 513, 702, 1611, 1655, 2276
BsmAI GTCTC 7 cut(s) 172, 590, 794, 1461, 1635, 1770, 1940
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 524, 1156, 1731, 1998
BsmI GAATGC 2 cut(s) 1093, 2641
BsnI GGCC 4 cut(s) 9, 476, 1010, 1701
BsoBI CYCGRG 1 cut(s) 2349
Bsp119I TTCGAA 2 cut(s) 271, 927
Bsp1286I GDGCHC 2 cut(s) 318, 1268
Bsp143I GATC 4 cut(s) 534, 1513, 2076, 2485
BspANI GGCC 4 cut(s) 9, 476, 1010, 1701
BspCNI CTCAG 3 cut(s) 630, 1261, 2356
BspFNI CGCG 2 cut(s) 449, 524
BspLI GGNNCC 8 cut(s) 298, 694, 715, 1257, 1432, 1616, 1719, 2209
BspMAI CTGCAG 1 cut(s) 1182
BspQI GCTCTTC 1 cut(s) 1256
BspT104I TTCGAA 2 cut(s) 271, 927
BspT107I GGYRCC 2 cut(s) 1430, 1614
BspTI CTTAAG 3 cut(s) 902, 1936, 2621
BsrBI CCGCTC 1 cut(s) 1981
BsrDI GCAATG 4 cut(s) 875, 1321, 2211, 2568
BsrI ACTGG 4 cut(s) 360, 633, 1451, 2167
BssECI CCNNGG 8 cut(s) 111, 310, 429, 507, 1525, 2287, 2349, 2510
BssMI GATC 4 cut(s) 534, 1513, 2076, 2485
BssNI GRCGYC 3 cut(s) 390, 441, 504
BssSI CACGAG 1 cut(s) 1353
BssT1I CCWWGG 1 cut(s) 2287
Bst2BI CACGAG 1 cut(s) 1353
Bst2UI CCWGG 6 cut(s) 113, 311, 727, 1526, 2072, 2512
Bst6I CTCTTC 7 cut(s) 109, 333, 924, 930, 1100, 1256, 1633
BstACI GRCGYC 3 cut(s) 390, 441, 504
BstAFI CTTAAG 3 cut(s) 902, 1936, 2621
BstBI TTCGAA 2 cut(s) 271, 927
BstC8I GCNNGC 6 cut(s) 7, 663, 798, 960, 1699, 2445
BstDEI CTNAG 6 cut(s) 638, 1269, 1653, 2007, 2364, 2502
BstDSI CCRYGG 1 cut(s) 429
BstENI CCTNNNNNAGG 1 cut(s) 1609
BstF5I GGATG 7 cut(s) 249, 1601, 1819, 1987, 2253, 2395, 2520
BstFNI CGCG 2 cut(s) 449, 524
BstH2I RGCGCY 4 cut(s) 455, 726, 767, 1031
BstHHI GCGC 7 cut(s) 400, 454, 725, 766, 958, 1030, 2200
BstKTI GATC 4 cut(s) 537, 1516, 2079, 2488
BstMAI GTCTC 7 cut(s) 172, 590, 794, 1461, 1635, 1770, 1940
BstMBI GATC 4 cut(s) 534, 1513, 2076, 2485
BstMWI GCNNNNNNNGC 7 cut(s) 11, 65, 449, 473, 482, 749, 758
BstNI CCWGG 6 cut(s) 113, 311, 727, 1526, 2072, 2512
BstNSI RCATGY 2 cut(s) 665, 962
BstPAI GACNNNNGTC 2 cut(s) 2133, 2366
BstSFI CTRYAG 3 cut(s) 1178, 1633, 2052
BstUI CGCG 2 cut(s) 449, 524
BstV1I GCAGC 6 cut(s) 1164, 2037, 2203, 2332, 2417, 2585
BstV2I GAAGAC 3 cut(s) 674, 1333, 1500
BstX2I RGATCY 1 cut(s) 2076
BstXI CCANNNNNNTGG 1 cut(s) 1441
BstYI RGATCY 1 cut(s) 2076
BsuI GTATCC 1 cut(s) 1438
BsuRI GGCC 4 cut(s) 9, 476, 1010, 1701
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 2 cut(s) 91, 481
BtsCI GGATG 7 cut(s) 249, 1601, 1819, 1987, 2253, 2395, 2520
BtsI GCAGTG 4 cut(s) 21, 825, 969, 1544
BtsIMutI CAGTG 8 cut(s) 21, 353, 825, 969, 1023, 1544, 2060, 2111
Cac8I GCNNGC 6 cut(s) 7, 663, 798, 960, 1699, 2445
CfoI GCGC 7 cut(s) 400, 454, 725, 766, 958, 1030, 2200
Cfr13I GGNCC 6 cut(s) 7, 406, 1255, 1700, 1718, 2368
Cfr9I CCCGGG 1 cut(s) 2349
CseI GACGC 3 cut(s) 379, 449, 512
Csp6I GTAC 8 cut(s) 255, 385, 517, 1157, 1350, 1615, 1965, 2256
CviAII CATG 6 cut(s) 662, 959, 1427, 1477, 1588, 2640
CviQI GTAC 8 cut(s) 255, 385, 517, 1157, 1350, 1615, 1965, 2256
DdeI CTNAG 6 cut(s) 638, 1269, 1653, 2007, 2364, 2502
DpnI GATC 4 cut(s) 536, 1515, 2078, 2487
DpnII GATC 4 cut(s) 534, 1513, 2076, 2485
DraI TTTAAA 1 cut(s) 1609
DriI GACNNNNNGTC 1 cut(s) 1891
Eam1104I CTCTTC 7 cut(s) 109, 333, 924, 930, 1100, 1256, 1633
Eam1105I GACNNNNNGTC 1 cut(s) 1891
EarI CTCTTC 7 cut(s) 109, 333, 924, 930, 1100, 1256, 1633
EciI GGCGGA 3 cut(s) 46, 97, 127
Ecl136II GAGCTC 1 cut(s) 1266
Eco130I CCWWGG 1 cut(s) 2287
Eco24I GRGCYC 2 cut(s) 318, 1268
Eco47I GGWCC 4 cut(s) 406, 1255, 1718, 2368
Eco53kI GAGCTC 1 cut(s) 1266
Eco57I CTGAAG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 2349
EcoICRI GAGCTC 1 cut(s) 1266
EcoNI CCTNNNNNAGG 1 cut(s) 1609
EcoO109I RGGNCCY 1 cut(s) 2368
EcoRI GAATTC 2 cut(s) 685, 2450
EcoRII CCWGG 6 cut(s) 111, 309, 725, 1524, 2070, 2510
EcoT14I CCWWGG 1 cut(s) 2287
EcoT22I ATGCAT 1 cut(s) 2641
EcoT38I GRGCYC 2 cut(s) 318, 1268
ErhI CCWWGG 1 cut(s) 2287
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 6 cut(s) 665, 962, 1430, 1480, 1591, 2643
FaqI GGGAC 4 cut(s) 524, 1156, 1731, 1998
FatI CATG 6 cut(s) 661, 958, 1426, 1476, 1587, 2639
FauI CCCGC 2 cut(s) 478, 1974
FblI GTMKAC 2 cut(s) 63, 1632
FokI GGATG 7 cut(s) 236, 1588, 1826, 1974, 2260, 2402, 2527
FriOI GRGCYC 2 cut(s) 318, 1268
FspBI CTAG 8 cut(s) 546, 672, 876, 1323, 1575, 1869, 2372, 2402
FspI TGCGCA 2 cut(s) 957, 2199
GlaI GCGC 7 cut(s) 399, 453, 724, 765, 957, 1029, 2199
GsaI CCCAGC 1 cut(s) 2580
GsuI CTGGAG 6 cut(s) 377, 711, 1052, 1434, 2093, 2184
HaeII RGCGCY 4 cut(s) 455, 726, 767, 1031
HaeIII GGCC 4 cut(s) 9, 476, 1010, 1701
HapII CCGG 6 cut(s) 266, 294, 507, 718, 793, 2350
HgaI GACGC 3 cut(s) 379, 449, 512
HhaI GCGC 7 cut(s) 400, 454, 725, 766, 958, 1030, 2200
Hin1I GRCGYC 3 cut(s) 390, 441, 504
Hin1II CATG 6 cut(s) 665, 962, 1430, 1480, 1591, 2643
Hin6I GCGC 7 cut(s) 398, 452, 723, 764, 956, 1028, 2198
HinP1I GCGC 7 cut(s) 398, 452, 723, 764, 956, 1028, 2198
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 3 cut(s) 886, 1337, 2009
HpaII CCGG 6 cut(s) 266, 294, 507, 718, 793, 2350
HphI GGTGA 8 cut(s) 544, 893, 1079, 1196, 1306, 1790, 2432, 2540
Hpy166II GTNNAC 5 cut(s) 64, 429, 502, 1633, 2225
Hpy8I GTNNAC 5 cut(s) 64, 429, 502, 1633, 2225
Hpy99I CGWCG 3 cut(s) 74, 449, 482
HpyCH4IV ACGT 2 cut(s) 90, 480
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 65, 449, 473, 482, 749, 758
HpyF3I CTNAG 6 cut(s) 638, 1269, 1653, 2007, 2364, 2502
HpySE526I ACGT 2 cut(s) 90, 480
Hsp92I GRCGYC 3 cut(s) 390, 441, 504
Hsp92II CATG 6 cut(s) 665, 962, 1430, 1480, 1591, 2643
HspAI GCGC 7 cut(s) 398, 452, 723, 764, 956, 1028, 2198
KpnI GGTACC 1 cut(s) 1618
Kzo9I GATC 4 cut(s) 534, 1513, 2076, 2485
LguI GCTCTTC 1 cut(s) 1256
LmnI GCTCC 8 cut(s) 302, 679, 692, 720, 979, 1037, 1237, 2317
Lsp1109I GCAGC 6 cut(s) 1164, 2037, 2203, 2332, 2417, 2585
LweI GCATC 1 cut(s) 2047
MaeI CTAG 8 cut(s) 546, 672, 876, 1323, 1575, 1869, 2372, 2402
MaeII ACGT 2 cut(s) 90, 480
MaeIII GTNAC 5 cut(s) 91, 997, 1355, 2100, 2470
MalI GATC 4 cut(s) 536, 1515, 2078, 2487
MbiI CCGCTC 1 cut(s) 1981
MboI GATC 4 cut(s) 534, 1513, 2076, 2485
MflI RGATCY 1 cut(s) 2076
MhlI GDGCHC 2 cut(s) 318, 1268
MlyI GAGTC 8 cut(s) 33, 95, 114, 841, 1323, 1490, 2143, 2175
MmeI TCCRAC 6 cut(s) 7, 711, 786, 1200, 1791, 1803
Mph1103I ATGCAT 1 cut(s) 2641
MslI CAYNNNNRTG 2 cut(s) 1016, 1475
MspA1I CMGCKG 1 cut(s) 2576
MspCI CTTAAG 3 cut(s) 902, 1936, 2621
MspI CCGG 6 cut(s) 266, 294, 507, 718, 793, 2350
Mva1269I GAATGC 2 cut(s) 1093, 2641
MvaI CCWGG 6 cut(s) 113, 311, 727, 1526, 2072, 2512
MvnI CGCG 2 cut(s) 449, 524
MwoI GCNNNNNNNGC 7 cut(s) 11, 65, 449, 473, 482, 749, 758
NciI CCSGG 5 cut(s) 266, 508, 718, 2350, 2351
NdeII GATC 4 cut(s) 534, 1513, 2076, 2485
NlaIII CATG 6 cut(s) 665, 962, 1430, 1480, 1591, 2643
NlaIV GGNNCC 8 cut(s) 298, 694, 715, 1257, 1432, 1616, 1719, 2209
NmuCI GTSAC 5 cut(s) 91, 997, 1355, 2100, 2470
NsbI TGCGCA 2 cut(s) 957, 2199
NsiI ATGCAT 1 cut(s) 2641
NspI RCATGY 2 cut(s) 665, 962
NspV TTCGAA 2 cut(s) 271, 927
PaeI GCATGC 2 cut(s) 665, 962
PciSI GCTCTTC 1 cut(s) 1256
PcsI WCGNNNNNNNCGW 3 cut(s) 324, 333, 617
PctI GAATGC 2 cut(s) 1093, 2641
PfeI GAWTC 7 cut(s) 200, 273, 371, 616, 2338, 2498, 2506
Pfl23II CGTACG 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 2276
PfoI TCCNGGA 1 cut(s) 716
PleI GAGTC 8 cut(s) 32, 95, 114, 840, 1323, 1490, 2142, 2174
PpsI GAGTC 8 cut(s) 32, 95, 114, 840, 1323, 1490, 2142, 2174
PpuMI RGGWCCY 1 cut(s) 2368
PshAI GACNNNNGTC 2 cut(s) 2133, 2366
Psp124BI GAGCTC 1 cut(s) 1268
Psp5II RGGWCCY 1 cut(s) 2368
Psp6I CCWGG 6 cut(s) 111, 309, 725, 1524, 2070, 2510
PspFI CCCAGC 1 cut(s) 2576
PspGI CCWGG 6 cut(s) 111, 309, 725, 1524, 2070, 2510
PspLI CGTACG 1 cut(s) 254
PspN4I GGNNCC 8 cut(s) 298, 694, 715, 1257, 1432, 1616, 1719, 2209
PspPI GGNCC 6 cut(s) 7, 406, 1255, 1700, 1718, 2368
PspPPI RGGWCCY 1 cut(s) 2368
PstI CTGCAG 1 cut(s) 1182
PsuI RGATCY 1 cut(s) 2076
PsyI GACNNNGTC 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 2576
RsaI GTAC 8 cut(s) 256, 386, 518, 1158, 1351, 1616, 1966, 2257
RsaNI GTAC 8 cut(s) 255, 385, 517, 1157, 1350, 1615, 1965, 2256
RseI CAYNNNNRTG 2 cut(s) 1016, 1475
SacI GAGCTC 1 cut(s) 1268
SapI GCTCTTC 1 cut(s) 1256
Sau3AI GATC 4 cut(s) 534, 1513, 2076, 2485
Sau96I GGNCC 6 cut(s) 7, 406, 1255, 1700, 1718, 2368
SchI GAGTC 8 cut(s) 33, 95, 114, 841, 1323, 1490, 2143, 2175
SduI GDGCHC 2 cut(s) 318, 1268
SfaNI GCATC 1 cut(s) 2047
SfcI CTRYAG 3 cut(s) 1178, 1633, 2052
SfuI TTCGAA 2 cut(s) 271, 927
SinI GGWCC 4 cut(s) 406, 1255, 1718, 2368
SmaI CCCGGG 1 cut(s) 2351
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1475
SmlI CTYRAG 7 cut(s) 149, 303, 889, 902, 1936, 2131, 2621
SmoI CTYRAG 7 cut(s) 149, 303, 889, 902, 1936, 2131, 2621
SphI GCATGC 2 cut(s) 665, 962
SspI AATATT 1 cut(s) 1771
SspMI CTAG 8 cut(s) 546, 672, 876, 1323, 1575, 1869, 2372, 2402
SstI GAGCTC 1 cut(s) 1268
StyI CCWWGG 1 cut(s) 2287
TaiI ACGT 2 cut(s) 93, 483
TaqII GACCGA 1 cut(s) 350
TauI GCSGC 8 cut(s) 143, 281, 284, 422, 452, 524, 761, 764
TfiI GAWTC 7 cut(s) 200, 273, 371, 616, 2338, 2498, 2506
TscAI CASTG 8 cut(s) 21, 360, 825, 969, 1023, 1551, 2067, 2111
TseFI GTSAC 5 cut(s) 91, 997, 1355, 2100, 2470
TseI GCWGC 6 cut(s) 1177, 2025, 2216, 2320, 2405, 2573
Tsp45I GTSAC 5 cut(s) 91, 997, 1355, 2100, 2470
TspDTI ATGAA 4 cut(s) 1332, 1604, 2037, 2190
TspGWI ACGGA 2 cut(s) 181, 242
TspMI CCCGGG 1 cut(s) 2349
TspRI CASTG 8 cut(s) 21, 360, 825, 969, 1023, 1551, 2067, 2111
Tth111I GACNNNGTC 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 2276
Vha464I CTTAAG 3 cut(s) 902, 1936, 2621
VpaK11BI GGWCC 4 cut(s) 406, 1255, 1718, 2368
XagI CCTNNNNNAGG 1 cut(s) 1609
XapI RAATTY 5 cut(s) 289, 624, 685, 1850, 2450
XceI RCATGY 2 cut(s) 665, 962
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmaI CCCGGG 1 cut(s) 2349
XmiI GTMKAC 2 cut(s) 63, 1632
XspI CTAG 8 cut(s) 546, 672, 876, 1323, 1575, 1869, 2372, 2402
Zsp2I ATGCAT 1 cut(s) 2641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.