RchiOBHm_Chr6g0251201

Histidine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008801
Physical Location & Seq
Reverse (-)
6502243 .. 6508678
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22521

Sequence Viewer

Length: 2646 bp
ATGGCGGGCCTTGCAGTGATAAGAGTCGGAGGCAAAGGCTCATCTCTCTCCTCCTCCGCCGTCTACGCCGTCGCCAATGCCCTCGCTCACGTCACTATCGACTCTTCCGCCCTCGACAGACTCTCCTCCTCCTCCTCCTCCGCCGCCAATCTCAAGCCTTACCACCTCGTCTCTCTCCTCCCTTCCTTCCCGTCCGTCCAAGAATCCAGAGCCTCCCTCGTTGTCCTCCTCGCCAAGCTCCTCTCCCTTAACATCCGTACGGTTCTCCCTGTTCGAATCGCCGCCGCCTTAAATTCCGGCTCCGCTCAAGACCTGGGGCTCGAAACCCTCGATTTGACCGAAGAGGAGGCTCTTGCGTTGGAGAAGCTCAAAGATTCGTCTGCTTTGTACGGCGTCTGTGCGCTTCTGGACCACCAATCGGCGGCTCTGTCCACGGTTTCGGACGCCGTCGCGGCGCTTTCTTGCGAGGCGTTGAAGGCCGACGTGGCGGGGTTTAATTCGGTTGACGCCGGGGACGGGTACGCCGCGAAGGAGGTGATCGGAGTTGCTAGTGATTTGAAGGTTTTGTTGAACGGGTCGAAGTTAGTCGGCAAAGTTGAGACTGAAGCTGTATCGGAGATTCCGAAAATTCACGCCAGTCTGAGAGTGCAAGTGAAATTGGTGCATGCGAAGACTAGGGAGGAGCTGAATTCTGGAGCCAAAGTAGGGAAAGGTGGTTCCCGGAGCGCCAGGTCGGAGAGGACGGCATTGCTGGCGTTGGCGGCGGCGCTGTTTGACTTGGGGGACTATAGTCTCCGAAGAGCGAAGTTGGATTTGGAGGCAGTGGGGAGTGAGAGTTTGCGTTTGAGATTGGTGGAGTTGTTTGGGAAGCAATGCCCTACTGGTGAAAGCTTGAGAGGTGGGCTTAAGTTGGTTCAGGAGTTGGCTTTCGAAGAGGAAGAGAATTATGTTAAGTTTGCGCATGCAGTGAATGTGTTGATGGAGCTTGTATGGAAGATTGTGACTTGGGAGGCAATAACAGTGTTTGTGGCGCTGGAGGGAGCAGAGTTGACTGGGAAGGGTCAAGGAGGTGAAGTGAATGGAGAAGTGAATGCGAAAACAGGGAAGAAGAGTGAGAAGAAGAAGAAGGCAGTTTTGGGGAAAGGGACAACTGTTATGGTACAGTTGATTAAAGATAAGCTGCAGGGTGAGGGAGGAAATGCTGTTGATTGTTCAGAGTTGTTGGAAAATTGGGTCAAGGAGCTTTTATTTTTTTTGGACCCGAAGAGCTCTGAGTTTGATGGTTTGTTGAACAAGGTGAAGGAGATAGTAGAAAGCAATGAAACTAGAAGACTCCCAAAGCTTCCAAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATAAGAAAGAAAGCATTTTCGATAATAGAAGATGTTTTTGAGAGGCATGGTGCCACAGCTCTGGATACTCCAGTTTTTGAATTGAGAGACACTCTCACGGGCAAATATGGGGAAGACTCGAAATTGATTTATGATCTTGCTGACCAGGGAGGAGAGCTTTCCTCACTGCGATATGATTTAACTGTTCCATTTGCTAGGTATGTTGCTATGAATGGCATCACATCCTTTAAAAGGTACCAAATAGCAAAGGTCTACAGAAGAGACAATCCCTCTAAGGGTAGATACCGTGAGTTCTACCAATGCGACTTTGATATTGCTGGCCAAAGTGAAAAAATGGGACCAGATTTTGAGGCTGTCTATGTGCTAACAGAACTTCTTGATGAGCTGAATATTGGAGACTACGAGGTTAAGTTAAATCACCGAAAGTTGTTGGATGGAATGTTGGAAATATGTGGAGTGCCTCCTGAAAAATTCAGAACTATTTGTTCTAGTATTGACAAGTTAGACAAGCAGTCATTCGAGCAAATAAAAAAAGAAATGGTGGAGGAGAAGGGCTTAAGTGTTGAGACAGCCGACAGAATTGGTACTTTTGTGAAAGAGCGGGGACATCCTTTGGAATTGTTATCTAAGCTTAAACAGGAGGGCAGCGAGTTCTTGGAGAACAATTCATCTATAGATGCACTGAATGACCTGGAGATCTTGTTCAAATGTTTGGAAAAGTCACAGTGTATTGGAAAAGTGGTTTTTGACTTGAGTCTTGCAAGGGGTCTTGATTATTATACTGGAGTCATATATGAAGCTGTTTTCAAGGGTGGTGCGCAGATGGGTTCCATTGCTGCTGGTGGACGATATGACAACCTTATAGGGATGTTCGGTACAAAGCAAGTTCCAGCAGTTGGTGTCAGCCTTGGAATTGAGCGAGTATTTGCTATAATGGAGCAGCTTCACAAAGACCAGAATCAGACAACCCGGGCAACCAAGACTGAGGTCCTAGTGAGTATATTAGGGGATGATATAGGACTAGCAGCAGAGCTATCAGGTGAGTTGTGGAAGGGAAAAGTGCAAGCAGAATTCTTGGTCAATAAGAGAGTGACGAAGCACTTTGATCGAGCAAAGGATTCTAAGATTCCCTGGATGATAATTGTGGGTGAAAAAGAGCAGAATGAAGGGGTTGTTCGATTGAAGAACATTGCAGCTGGGGAAGAAGTCGTAATTCAGAGAAGTGATATTGTTGAAGAACTTAAGAAGCGGTTGAATGCATGA

Protein Analysis

881

Amino Acids

96.16

Weight (kDa)

5.85

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_aromatic PF00221 103 - 231 2.3e-06 Aromatic amino acid lyase
tRNA-synt_His PF13393 451 - 769 1.1e-39 Histidyl-tRNA synthetase
HGTP_anticodon2 PF12745 787 - 879 1.4e-06 Anticodon binding domain of tRNAs
HGTP_anticodon PF03129 801 - 878 9.3e-13 Anticodon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 960, 2202
Acc65I GGTACC 1 cut(s) 1617
AccB1I GGYRCC 2 cut(s) 1433, 1617
AccB7I CCANNNNNTGG 1 cut(s) 2279
AccBSI CCGCTC 2 cut(s) 305, 1984
AccI GTMKAC 2 cut(s) 63, 1635
AccII CGCG 2 cut(s) 452, 527
AcoI YGGCCR 1 cut(s) 1702
AcsI RAATTY 5 cut(s) 292, 627, 688, 1853, 2453
AcuI CTGAAG 1 cut(s) 624
AcyI GRCGYC 3 cut(s) 393, 444, 507
AfaI GTAC 8 cut(s) 259, 389, 521, 1161, 1354, 1619, 1969, 2260
AfiI CCNNNNNNNGG 7 cut(s) 418, 421, 516, 705, 1614, 1658, 2279
AflII CTTAAG 3 cut(s) 905, 1939, 2624
AhdI GACNNNNNGTC 1 cut(s) 1894
AjiI CACGTC 2 cut(s) 91, 484
AjnI CCWGG 5 cut(s) 312, 728, 1527, 2073, 2513
AjuI GAANNNNNNNTTGG 4 cut(s) 797, 829, 1118, 1150
AloI GAACNNNNNNTCC 4 cut(s) 700, 732, 2069, 2101
Alw21I GWGCWC 1 cut(s) 1271
Alw26I GTCTC 7 cut(s) 175, 593, 797, 1464, 1638, 1773, 1943
Ama87I CYCGRG 1 cut(s) 2352
AoxI GGCC 3 cut(s) 7, 477, 1702
ApeKI GCWGC 6 cut(s) 1180, 2028, 2219, 2323, 2408, 2576
ApoI RAATTY 5 cut(s) 292, 627, 688, 1853, 2453
Asp718I GGTACC 1 cut(s) 1617
AspLEI GCGC 7 cut(s) 403, 457, 728, 769, 961, 1033, 2203
AspS9I GGNCC 5 cut(s) 7, 409, 1258, 1721, 2371
AsuC2I CCSGG 4 cut(s) 511, 721, 2353, 2354
AsuHPI GGTGA 8 cut(s) 547, 896, 1082, 1199, 1309, 1793, 2435, 2543
AsuII TTCGAA 2 cut(s) 274, 930
AvaI CYCGRG 1 cut(s) 2352
AvaII GGWCC 4 cut(s) 409, 1258, 1721, 2371
BaeI ACNNNNGTAYC 4 cut(s) 1440, 1473, 1959, 1992
BalI TGGCCA 1 cut(s) 1704
BanI GGYRCC 2 cut(s) 1433, 1617
BanII GRGCYC 2 cut(s) 321, 1271
BauI CACGAG 1 cut(s) 1356
BbsI GAAGAC 3 cut(s) 677, 1336, 1503
Bbv12I GWGCWC 1 cut(s) 1271
BbvI GCAGC 6 cut(s) 1167, 2040, 2206, 2335, 2420, 2588
BccI CCATC 4 cut(s) 973, 1274, 1811, 2200
BceAI ACGGC 5 cut(s) 44, 53, 406, 431, 759
BcgI CGANNNNNNTGC 2 cut(s) 2471, 2505
BciT130I CCWGG 5 cut(s) 314, 730, 1529, 2075, 2515
BciVI GTATCC 1 cut(s) 1441
BcnI CCSGG 4 cut(s) 511, 721, 2353, 2354
BcoDI GTCTC 7 cut(s) 175, 593, 797, 1464, 1638, 1773, 1943
BfaI CTAG 7 cut(s) 549, 675, 1326, 1578, 1872, 2375, 2405
BfmI CTRYAG 4 cut(s) 787, 1181, 1636, 2055
BfoI RGCGCY 4 cut(s) 458, 729, 770, 1034
BfrI CTTAAG 3 cut(s) 905, 1939, 2624
BfuI GTATCC 1 cut(s) 1441
BglI GCCNNNNNGGC 2 cut(s) 452, 485
BglII AGATCT 1 cut(s) 2079
Bme1390I CCNGG 9 cut(s) 314, 511, 721, 730, 1529, 2075, 2353, 2354, 2515
Bme18I GGWCC 4 cut(s) 409, 1258, 1721, 2371
BmeRI GACNNNNNGTC 1 cut(s) 1894
BmeT110I CYCGRG 1 cut(s) 2352
BmgBI CACGTC 2 cut(s) 91, 484
BmgT120I GGNCC 5 cut(s) 7, 409, 1258, 1721, 2371
BmiI GGNNCC 8 cut(s) 301, 697, 718, 1260, 1435, 1619, 1722, 2212
BmrFI CCNGG 9 cut(s) 314, 511, 721, 730, 1529, 2075, 2353, 2354, 2515
BmrI ACTGGG 1 cut(s) 1062
BmsI GCATC 2 cut(s) 1608, 2050
BmuI ACTGGG 1 cut(s) 1062
BoxI GACNNNNGTC 3 cut(s) 789, 2136, 2369
BpiI GAAGAC 3 cut(s) 677, 1336, 1503
BplI GAGNNNNNCTC 8 cut(s) 201, 233, 1459, 1491, 1461, 1493, 1529, 1561
BpmI CTGGAG 5 cut(s) 714, 1055, 1437, 2096, 2187
Bpu14I TTCGAA 2 cut(s) 274, 930
BpuEI CTTGAG 4 cut(s) 137, 291, 913, 2155
BpuMI CCSGG 4 cut(s) 511, 721, 2353, 2354
BsaHI GRCGYC 3 cut(s) 393, 444, 507
BsaJI CCNNGG 7 cut(s) 313, 432, 510, 1528, 2290, 2352, 2513
Bsc4I CCNNNNNNNGG 7 cut(s) 418, 421, 516, 705, 1614, 1658, 2279
Bse1I ACTGG 5 cut(s) 636, 886, 1057, 1454, 2170
Bse3DI GCAATG 5 cut(s) 746, 878, 1324, 2214, 2571
BseBI CCWGG 5 cut(s) 314, 730, 1529, 2075, 2515
BseDI CCNNGG 7 cut(s) 313, 432, 510, 1528, 2290, 2352, 2513
BseGI GGATG 7 cut(s) 252, 1604, 1822, 1990, 2256, 2398, 2523
BseLI CCNNNNNNNGG 7 cut(s) 418, 421, 516, 705, 1614, 1658, 2279
BseMI GCAATG 5 cut(s) 746, 878, 1324, 2214, 2571
BseMII CTCAG 3 cut(s) 632, 1263, 2358
BseNI ACTGG 5 cut(s) 636, 886, 1057, 1454, 2170
BseXI GCAGC 6 cut(s) 1167, 2040, 2206, 2335, 2420, 2588
BseYI CCCAGC 1 cut(s) 2579
Bsh1236I CGCG 2 cut(s) 452, 527
BshFI GGCC 3 cut(s) 9, 479, 1704
BshNI GGYRCC 2 cut(s) 1433, 1617
BsiHKAI GWGCWC 1 cut(s) 1271
BsiHKCI CYCGRG 1 cut(s) 2352
BsiSI CCGG 4 cut(s) 297, 510, 721, 2353
BsiWI CGTACG 1 cut(s) 257
BslFI GGGAC 5 cut(s) 527, 797, 1159, 1734, 2001
BslI CCNNNNNNNGG 7 cut(s) 418, 421, 516, 705, 1614, 1658, 2279
BsmAI GTCTC 7 cut(s) 175, 593, 797, 1464, 1638, 1773, 1943
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 5 cut(s) 527, 797, 1159, 1734, 2001
BsmI GAATGC 2 cut(s) 1096, 2644
BsnI GGCC 3 cut(s) 9, 479, 1704
BsoBI CYCGRG 1 cut(s) 2352
Bsp119I TTCGAA 2 cut(s) 274, 930
Bsp1286I GDGCHC 2 cut(s) 321, 1271
Bsp143I GATC 4 cut(s) 537, 1516, 2079, 2488
BspANI GGCC 3 cut(s) 9, 479, 1704
BspCNI CTCAG 3 cut(s) 633, 1264, 2359
BspFNI CGCG 2 cut(s) 452, 527
BspLI GGNNCC 8 cut(s) 301, 697, 718, 1260, 1435, 1619, 1722, 2212
BspMAI CTGCAG 1 cut(s) 1185
BspQI GCTCTTC 2 cut(s) 793, 1259
BspT104I TTCGAA 2 cut(s) 274, 930
BspT107I GGYRCC 2 cut(s) 1433, 1617
BspTI CTTAAG 3 cut(s) 905, 1939, 2624
BsrBI CCGCTC 2 cut(s) 305, 1984
BsrDI GCAATG 5 cut(s) 746, 878, 1324, 2214, 2571
BsrI ACTGG 5 cut(s) 636, 886, 1057, 1454, 2170
BssECI CCNNGG 7 cut(s) 313, 432, 510, 1528, 2290, 2352, 2513
BssMI GATC 4 cut(s) 537, 1516, 2079, 2488
BssNI GRCGYC 3 cut(s) 393, 444, 507
BssSI CACGAG 1 cut(s) 1356
BssT1I CCWWGG 1 cut(s) 2290
Bst2BI CACGAG 1 cut(s) 1356
Bst2UI CCWGG 5 cut(s) 314, 730, 1529, 2075, 2515
Bst4CI ACNGT 8 cut(s) 262, 436, 1021, 1153, 1164, 1567, 1670, 2109
Bst6I CTCTTC 8 cut(s) 109, 336, 793, 927, 933, 1103, 1259, 1636
BstACI GRCGYC 3 cut(s) 393, 444, 507
BstAFI CTTAAG 3 cut(s) 905, 1939, 2624
BstBI TTCGAA 2 cut(s) 274, 930
BstC8I GCNNGC 6 cut(s) 7, 666, 753, 963, 1702, 2448
BstDEI CTNAG 6 cut(s) 641, 1272, 1656, 2010, 2367, 2505
BstDSI CCRYGG 1 cut(s) 432
BstENI CCTNNNNNAGG 1 cut(s) 1612
BstF5I GGATG 7 cut(s) 252, 1604, 1822, 1990, 2256, 2398, 2523
BstFNI CGCG 2 cut(s) 452, 527
BstH2I RGCGCY 4 cut(s) 458, 729, 770, 1034
BstHHI GCGC 7 cut(s) 403, 457, 728, 769, 961, 1033, 2203
BstKTI GATC 4 cut(s) 540, 1519, 2082, 2491
BstMAI GTCTC 7 cut(s) 175, 593, 797, 1464, 1638, 1773, 1943
BstMBI GATC 4 cut(s) 537, 1516, 2079, 2488
BstMWI GCNNNNNNNGC 7 cut(s) 11, 65, 452, 476, 485, 752, 761
BstNI CCWGG 5 cut(s) 314, 730, 1529, 2075, 2515
BstNSI RCATGY 2 cut(s) 668, 965
BstPAI GACNNNNGTC 3 cut(s) 789, 2136, 2369
BstSCI CCNGG 9 cut(s) 312, 509, 719, 728, 1527, 2073, 2351, 2352, 2513
BstSFI CTRYAG 4 cut(s) 787, 1181, 1636, 2055
BstUI CGCG 2 cut(s) 452, 527
BstV1I GCAGC 6 cut(s) 1167, 2040, 2206, 2335, 2420, 2588
BstV2I GAAGAC 3 cut(s) 677, 1336, 1503
BstX2I RGATCY 1 cut(s) 2079
BstXI CCANNNNNNTGG 1 cut(s) 1444
BstYI RGATCY 1 cut(s) 2079
BsuI GTATCC 1 cut(s) 1441
BsuRI GGCC 3 cut(s) 9, 479, 1704
BtgI CCRYGG 1 cut(s) 432
BtrI CACGTC 2 cut(s) 91, 484
BtsCI GGATG 7 cut(s) 252, 1604, 1822, 1990, 2256, 2398, 2523
BtsI GCAGTG 4 cut(s) 21, 828, 972, 1547
BtsIMutI CAGTG 7 cut(s) 21, 828, 972, 1026, 1547, 2063, 2114
Cac8I GCNNGC 6 cut(s) 7, 666, 753, 963, 1702, 2448
CfoI GCGC 7 cut(s) 403, 457, 728, 769, 961, 1033, 2203
Cfr13I GGNCC 5 cut(s) 7, 409, 1258, 1721, 2371
Cfr9I CCCGGG 1 cut(s) 2352
CseI GACGC 3 cut(s) 382, 452, 515
Csp6I GTAC 8 cut(s) 258, 388, 520, 1160, 1353, 1618, 1968, 2259
CviAII CATG 4 cut(s) 665, 962, 1430, 2643
CviQI GTAC 8 cut(s) 258, 388, 520, 1160, 1353, 1618, 1968, 2259
DdeI CTNAG 6 cut(s) 641, 1272, 1656, 2010, 2367, 2505
DpnI GATC 4 cut(s) 539, 1518, 2081, 2490
DpnII GATC 4 cut(s) 537, 1516, 2079, 2488
DraI TTTAAA 1 cut(s) 1612
DriI GACNNNNNGTC 1 cut(s) 1894
EaeI YGGCCR 1 cut(s) 1702
Eam1104I CTCTTC 8 cut(s) 109, 336, 793, 927, 933, 1103, 1259, 1636
Eam1105I GACNNNNNGTC 1 cut(s) 1894
EarI CTCTTC 8 cut(s) 109, 336, 793, 927, 933, 1103, 1259, 1636
EciI GGCGGA 3 cut(s) 46, 97, 130
Ecl136II GAGCTC 1 cut(s) 1269
Eco130I CCWWGG 1 cut(s) 2290
Eco24I GRGCYC 2 cut(s) 321, 1271
Eco47I GGWCC 4 cut(s) 409, 1258, 1721, 2371
Eco53kI GAGCTC 1 cut(s) 1269
Eco57I CTGAAG 1 cut(s) 624
Eco88I CYCGRG 1 cut(s) 2352
EcoICRI GAGCTC 1 cut(s) 1269
EcoNI CCTNNNNNAGG 1 cut(s) 1612
EcoO109I RGGNCCY 1 cut(s) 2371
EcoRI GAATTC 2 cut(s) 688, 2453
EcoRII CCWGG 5 cut(s) 312, 728, 1527, 2073, 2513
EcoT14I CCWWGG 1 cut(s) 2290
EcoT22I ATGCAT 1 cut(s) 2644
EcoT38I GRGCYC 2 cut(s) 321, 1271
ErhI CCWWGG 1 cut(s) 2290
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 4 cut(s) 668, 965, 1433, 2646
FaqI GGGAC 5 cut(s) 527, 797, 1159, 1734, 2001
FatI CATG 4 cut(s) 664, 961, 1429, 2642
FauI CCCGC 2 cut(s) 481, 1977
FblI GTMKAC 2 cut(s) 63, 1635
FokI GGATG 7 cut(s) 239, 1591, 1829, 1977, 2263, 2405, 2530
FriOI GRGCYC 2 cut(s) 321, 1271
FspBI CTAG 7 cut(s) 549, 675, 1326, 1578, 1872, 2375, 2405
FspI TGCGCA 2 cut(s) 960, 2202
GlaI GCGC 7 cut(s) 402, 456, 727, 768, 960, 1032, 2202
GsaI CCCAGC 1 cut(s) 2583
GsuI CTGGAG 5 cut(s) 714, 1055, 1437, 2096, 2187
HaeII RGCGCY 4 cut(s) 458, 729, 770, 1034
HaeIII GGCC 3 cut(s) 9, 479, 1704
HapII CCGG 4 cut(s) 297, 510, 721, 2353
HgaI GACGC 3 cut(s) 382, 452, 515
HhaI GCGC 7 cut(s) 403, 457, 728, 769, 961, 1033, 2203
Hin1I GRCGYC 3 cut(s) 393, 444, 507
Hin1II CATG 4 cut(s) 668, 965, 1433, 2646
Hin6I GCGC 7 cut(s) 401, 455, 726, 767, 959, 1031, 2201
HinP1I GCGC 7 cut(s) 401, 455, 726, 767, 959, 1031, 2201
HincII GTYRAC 2 cut(s) 505, 1050
HindII GTYRAC 2 cut(s) 505, 1050
HindIII AAGCTT 3 cut(s) 889, 1340, 2012
HpaII CCGG 4 cut(s) 297, 510, 721, 2353
HphI GGTGA 8 cut(s) 547, 896, 1082, 1199, 1309, 1793, 2435, 2543
Hpy166II GTNNAC 6 cut(s) 64, 432, 505, 1050, 1636, 2228
Hpy8I GTNNAC 6 cut(s) 64, 432, 505, 1050, 1636, 2228
Hpy99I CGWCG 3 cut(s) 74, 452, 485
HpyCH4III ACNGT 8 cut(s) 262, 436, 1021, 1153, 1164, 1567, 1670, 2109
HpyCH4IV ACGT 2 cut(s) 90, 483
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 65, 452, 476, 485, 752, 761
HpyF3I CTNAG 6 cut(s) 641, 1272, 1656, 2010, 2367, 2505
HpySE526I ACGT 2 cut(s) 90, 483
Hsp92I GRCGYC 3 cut(s) 393, 444, 507
Hsp92II CATG 4 cut(s) 668, 965, 1433, 2646
HspAI GCGC 7 cut(s) 401, 455, 726, 767, 959, 1031, 2201
KpnI GGTACC 1 cut(s) 1621
Kzo9I GATC 4 cut(s) 537, 1516, 2079, 2488
LguI GCTCTTC 2 cut(s) 793, 1259
LmnI GCTCC 9 cut(s) 243, 305, 682, 695, 723, 982, 1040, 1240, 2320
Lsp1109I GCAGC 6 cut(s) 1167, 2040, 2206, 2335, 2420, 2588
LweI GCATC 2 cut(s) 1608, 2050
MaeI CTAG 7 cut(s) 549, 675, 1326, 1578, 1872, 2375, 2405
MaeII ACGT 2 cut(s) 90, 483
MaeIII GTNAC 5 cut(s) 91, 1000, 1358, 2103, 2473
MalI GATC 4 cut(s) 539, 1518, 2081, 2490
MbiI CCGCTC 2 cut(s) 305, 1984
MboI GATC 4 cut(s) 537, 1516, 2079, 2488
MflI RGATCY 1 cut(s) 2079
MhlI GDGCHC 2 cut(s) 321, 1271
MlsI TGGCCA 1 cut(s) 1704
MluNI TGGCCA 1 cut(s) 1704
MlyI GAGTC 7 cut(s) 33, 95, 114, 1326, 1493, 2146, 2178
MmeI TCCRAC 7 cut(s) 7, 339, 714, 789, 1203, 1794, 1806
Mox20I TGGCCA 1 cut(s) 1704
Mph1103I ATGCAT 1 cut(s) 2644
MscI TGGCCA 1 cut(s) 1704
Msp20I TGGCCA 1 cut(s) 1704
MspA1I CMGCKG 1 cut(s) 2579
MspCI CTTAAG 3 cut(s) 905, 1939, 2624
MspI CCGG 4 cut(s) 297, 510, 721, 2353
MspR9I CCNGG 9 cut(s) 314, 511, 721, 730, 1529, 2075, 2353, 2354, 2515
Mva1269I GAATGC 2 cut(s) 1096, 2644
MvaI CCWGG 5 cut(s) 314, 730, 1529, 2075, 2515
MvnI CGCG 2 cut(s) 452, 527
MwoI GCNNNNNNNGC 7 cut(s) 11, 65, 452, 476, 485, 752, 761
NciI CCSGG 4 cut(s) 511, 721, 2353, 2354
NdeII GATC 4 cut(s) 537, 1516, 2079, 2488
NlaIII CATG 4 cut(s) 668, 965, 1433, 2646
NlaIV GGNNCC 8 cut(s) 301, 697, 718, 1260, 1435, 1619, 1722, 2212
NmuCI GTSAC 5 cut(s) 91, 1000, 1358, 2103, 2473
NsbI TGCGCA 2 cut(s) 960, 2202
NsiI ATGCAT 1 cut(s) 2644
NspI RCATGY 2 cut(s) 668, 965
NspV TTCGAA 2 cut(s) 274, 930
PaeI GCATGC 2 cut(s) 668, 965
PciSI GCTCTTC 2 cut(s) 793, 1259
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 327, 336, 620
PctI GAATGC 2 cut(s) 1096, 2644
PfeI GAWTC 7 cut(s) 203, 276, 374, 619, 2341, 2501, 2509
Pfl23II CGTACG 1 cut(s) 257
PflFI GACNNNGTC 1 cut(s) 446
PflMI CCANNNNNTGG 1 cut(s) 2279
PfoI TCCNGGA 1 cut(s) 719
PleI GAGTC 7 cut(s) 32, 95, 114, 1326, 1493, 2145, 2177
PpsI GAGTC 7 cut(s) 32, 95, 114, 1326, 1493, 2145, 2177
PpuMI RGGWCCY 1 cut(s) 2371
PshAI GACNNNNGTC 3 cut(s) 789, 2136, 2369
Psp124BI GAGCTC 1 cut(s) 1271
Psp5II RGGWCCY 1 cut(s) 2371
Psp6I CCWGG 5 cut(s) 312, 728, 1527, 2073, 2513
PspFI CCCAGC 1 cut(s) 2579
PspGI CCWGG 5 cut(s) 312, 728, 1527, 2073, 2513
PspLI CGTACG 1 cut(s) 257
PspN4I GGNNCC 8 cut(s) 301, 697, 718, 1260, 1435, 1619, 1722, 2212
PspPI GGNCC 5 cut(s) 7, 409, 1258, 1721, 2371
PspPPI RGGWCCY 1 cut(s) 2371
PstI CTGCAG 1 cut(s) 1185
PsuI RGATCY 1 cut(s) 2079
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 2579
RsaI GTAC 8 cut(s) 259, 389, 521, 1161, 1354, 1619, 1969, 2260
RsaNI GTAC 8 cut(s) 258, 388, 520, 1160, 1353, 1618, 1968, 2259
SacI GAGCTC 1 cut(s) 1271
SapI GCTCTTC 2 cut(s) 793, 1259
Sau3AI GATC 4 cut(s) 537, 1516, 2079, 2488
Sau96I GGNCC 5 cut(s) 7, 409, 1258, 1721, 2371
SchI GAGTC 7 cut(s) 33, 95, 114, 1326, 1493, 2146, 2178
ScrFI CCNGG 9 cut(s) 314, 511, 721, 730, 1529, 2075, 2353, 2354, 2515
SduI GDGCHC 2 cut(s) 321, 1271
SfaNI GCATC 2 cut(s) 1608, 2050
SfcI CTRYAG 4 cut(s) 787, 1181, 1636, 2055
SfuI TTCGAA 2 cut(s) 274, 930
SinI GGWCC 4 cut(s) 409, 1258, 1721, 2371
SmaI CCCGGG 1 cut(s) 2354
SmlI CTYRAG 7 cut(s) 152, 306, 892, 905, 1939, 2134, 2624
SmoI CTYRAG 7 cut(s) 152, 306, 892, 905, 1939, 2134, 2624
SphI GCATGC 2 cut(s) 668, 965
SspI AATATT 1 cut(s) 1774
SspMI CTAG 7 cut(s) 549, 675, 1326, 1578, 1872, 2375, 2405
SstI GAGCTC 1 cut(s) 1271
StyD4I CCNGG 9 cut(s) 312, 509, 719, 728, 1527, 2073, 2351, 2352, 2513
StyI CCWWGG 1 cut(s) 2290
TaaI ACNGT 8 cut(s) 262, 436, 1021, 1153, 1164, 1567, 1670, 2109
TaiI ACGT 2 cut(s) 93, 486
TaqII GACCGA 1 cut(s) 353
TauI GCSGC 8 cut(s) 146, 284, 287, 425, 455, 527, 764, 767
TfiI GAWTC 7 cut(s) 203, 276, 374, 619, 2341, 2501, 2509
TscAI CASTG 7 cut(s) 21, 828, 972, 1026, 1554, 2070, 2114
TseFI GTSAC 5 cut(s) 91, 1000, 1358, 2103, 2473
TseI GCWGC 6 cut(s) 1180, 2028, 2219, 2323, 2408, 2576
Tsp45I GTSAC 5 cut(s) 91, 1000, 1358, 2103, 2473
TspDTI ATGAA 5 cut(s) 1335, 1607, 2040, 2193, 2562
TspGWI ACGGA 2 cut(s) 184, 245
TspMI CCCGGG 1 cut(s) 2352
TspRI CASTG 7 cut(s) 21, 828, 972, 1026, 1554, 2070, 2114
Tth111I GACNNNGTC 1 cut(s) 446
Van91I CCANNNNNTGG 1 cut(s) 2279
Vha464I CTTAAG 3 cut(s) 905, 1939, 2624
VpaK11BI GGWCC 4 cut(s) 409, 1258, 1721, 2371
XagI CCTNNNNNAGG 1 cut(s) 1612
XapI RAATTY 5 cut(s) 292, 627, 688, 1853, 2453
XceI RCATGY 2 cut(s) 668, 965
XmaI CCCGGG 1 cut(s) 2352
XmiI GTMKAC 2 cut(s) 63, 1635
XspI CTAG 7 cut(s) 549, 675, 1326, 1578, 1872, 2375, 2405
Zsp2I ATGCAT 1 cut(s) 2644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.