Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0008801

Physical Location & Seq

Genomic Coordinates Forward (+)

67557019 .. 67563179

Gene Structure 6161 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00216930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGCGGGCCTTGCAGTGATAAGAGTTGGAGGCAAAGGCTCATCTCTCTCCTCCTCCGCCGTCTACGCCGTCGCCAATGCCCTCGCTCACGTCACTATCGACTCTTCCGCCCTCGACAGACTCTCCTCCTCCTCCTCCGCCGCCGCCAATCTCAAGCCTTACCACCTCGTCTCTCTCCTCCCTTCCTTCCCGTCCGTCCAAGAATCCAGAGCCTCCCTCGTTGTCCTCCTCGCCAAGCTCCTCTCCCTCAACATCCGTACGGTTCTCCCTGTTCGAATCGCCGCCGCCCTAAATTCCAGCTCCACTCAAGACTTGGCGCTCGAAACCCTCGATTTGACCGAAGAGGAGGCTTTTGCGTTGGAGAAGCTCAAAGATTCGTCTGCTTTGTACGGCGTCTGTGCGCTTCTGGACCACCAATCGGCGGCTCTGTCCACGGTTTCGGACGCCGTCGCGGCGCTTTCTTGCGAGGCGTTGAAGGCCGACGTGGCGGGGTTTAATTCGGTCGACGCCGGGGACGGGTACGCCGCGAAGGAGGTGATCGGAGTTGCTAGTGATTTGAAGGTTTTGTTGAACGGGTCGAAGTTAGTCGGCAAAGTTGAGACTGAAGCTGTTTCGGAGATTCCGAAAATTCACGCCAGTCTGAGAGTGCAAGTGAAATTGGTGCATGCTAAGACTAGGGAGGAGCTGAATTCTGGAGCCAAAGTGGGGAAAGGTGGTTCCCGGAGCGCCAGGTCGGAGAGGACGGCATTGTTGGCGTTAGCGGCGGCGCTGTTTGACTTGGGGGATTACAGTCTCCGGCGAGCGAAGTTGGATTTGGAGGCAGTGGGGAGTGAGAGTTTGCGTTTGAGATTGGTGGAGTTGTTTGGGAAGCAATGCCCTAGTGGTGAAAGCTTGAGAAGTGGGCTTAAGCTGGTTCAGGAGTTGGCTTTCGAAGAGGAAGAGAATTATGTTAAGTTTGCGCATGCAGTGAATGTGTTGATGGAGCTTGTTTGGAAGATTGTGACTTGGGAGGCCATAACAGTGTTTGTGGCGCTGGAGGGAGCAGAGTTGAGTGGGAAGGGTCAAGGAGGTGAAGTGAATGGAGAAGTGAATGCGAAAGCAGGGAAGAAGAGTGAGAAGAAGAAGAAGGCGGTTTTGGGGAAAGGGACAACTGTTATGGTACAGTTGATTAGAGATAAGCTGCAGGGTGAGGGAGGAAATGCTGTTGATAGTTCAGAGTTGTTGGAAAATTGGGTCAAGGAGCTTTTATTGTTTTTGGACCCGAAGAGCTCTGAGTTTGATGGTTTGTTGAACAAGGTGAAGGAGATAGTAGAAAGCAATGAAACTAGAAGACTCCCAAAGCTTCCAAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATAAGAAAGAAAGCATTTTTGATAATAGAAGATGTTTTTGAGAGGCATGGTGCCACAGCTCTGGATACTCCAGTTTTTGAATTGAGAGACACTCTCATGGGAAAATATGGGGAAGACTCGAAATTGATTTATGATCTTGCTGACCAGGGAGGAGAGCTTTCCTCACTGCGATATGATTTAACTGTTCCATTTGCTAGGTATGTTGCTATGAATGGTATCACATCCTTTAAAAGGTACCAAATAGCAAAGGTCTACAGAAGAGACAATCCCTCTAAGGGTAGATACCGTGAGTTCTACCAATGCGACTTTGATATTGCTGGCCAAAGTGAAAAAATGGGGCCAGATTTTGAGGCTGTCTATGTTCTAACAGAACTTCTTGATGAGCTGAATATTGGAGACTACGAGGTTAAGTTAAATCACCGAAAGTTGTTGGATGGAATGTTGGAAATATGTGGAGTGCCTCCTGAAAAATTCAGAACTATTTGTTCTAGTATTGACAAGTTAGACAAGCAGTCATTCGAGCAAATAAAAAAAGAAATGGTGGAGGAGAAGGGCTTAAGTGTTGAGACAGCCGACAGAATTGGTACTTTTGTGAAAGAGCGGGGACATCCTTTGGAATTGTTATCTAAGCTTAAACAGGAGGGCAGCGAGTTCTTGGAGAACAATTCATCTATAGATGCACTGAATGACCTGGAGATCTTGTTCAAATGTTTGGAAAAGTCACAGTGTATTGGAAAAGTGGTTTTTGACTTGAGTCTTGCAAGGGGTCTTGATTATTATACTGGAGTCATATATGAAGCCGTTTTCAAGGGTGGTGCGCAGACAACCCGGGCAACCAAGACTGAGGTCCTAGTGAGTATATTAGGGGATGATATAGGACTAGCAGCAGAGCTATCAGGTGAGTTGTGGAAGGGAAAAGTGCAAGCAGAATTCTTGGTCAATAAGAGAGTGACGAAGCACTTTGATCGAGCAAAGGATTCTAAGATTCCCTGGATGATAATTGTGGGTGAAAAAGAGCAGAATGAAGGGGTTGTTCGATTGAAGAACATTGCAGCTGGGGAAGAAGTCGTAATTCAGAGAAGTGATATTGTTGAAGAACTTAAGAAGCGGTTGAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0032543 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0140053 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon Lyase_aromatic tRNA-synt_His

Protein Analysis

834

Amino Acids

91.22

Weight (kDa)

5.8

Isoelectric Point (pI)

33.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_His	PF13393	451 - 733	4.8e-28	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	740 - 832	1.3e-06	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	754 - 831	8.7e-13	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02760 AT3G02760
fragaria_vesca	FvH4_2g00780 FvH4_2g00780 FvH4_2g00780 FvH4_2g00780 FvH4_3g14280 FvH4_3g25240
malus_domestica	MD05G1003500.v1.1 MD10G1004600.v1.1
prunus_persica	Prupe.8G006900_v2.0.a1
pyrus_communis	pycom05g00320 pycom10g00360
rosa_chinensis	RchiOBHm_Chr6g0251201
rosa_laevigata	RLG00000015474
rosa_multiflora	Rmu_sc0004077.1_g000009
rosa_roxburghii	Rroxscaffold_7G00216930
rosa_rugosa	Rorug05G0500700.1
rosa_samantha	Rh6AG010100 Rh6BG001900 Rh6CG007000 Rh6DG007500
rosa_wichuraiana	Rw6G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	960, 2202
Acc65I	GGTACC	1 cut(s)	1617
AccB1I	GGYRCC	2 cut(s)	1433, 1617
AccBSI	CCGCTC	1 cut(s)	1984
AccI	GTMKAC	3 cut(s)	63, 504, 1635
AccII	CGCG	2 cut(s)	452, 527
AcoI	YGGCCR	1 cut(s)	1702
AcsI	RAATTY	5 cut(s)	292, 627, 688, 1853, 2312
AcuI	CTGAAG	1 cut(s)	624
AcyI	GRCGYC	3 cut(s)	393, 444, 507
AfaI	GTAC	7 cut(s)	259, 389, 521, 1161, 1354, 1619, 1969
AfiI	CCNNNNNNNGG	6 cut(s)	418, 421, 516, 705, 1614, 1658
AflII	CTTAAG	3 cut(s)	905, 1939, 2483
AhdI	GACNNNNNGTC	1 cut(s)	1894
AjiI	CACGTC	2 cut(s)	91, 484
AjnI	CCWGG	4 cut(s)	728, 1527, 2073, 2372
AjuI	GAANNNNNNNTTGG	4 cut(s)	797, 829, 1118, 1150
AloI	GAACNNNNNNTCC	4 cut(s)	700, 732, 2069, 2101
Alw21I	GWGCWC	1 cut(s)	1271
Alw26I	GTCTC	7 cut(s)	175, 593, 797, 1464, 1638, 1773, 1943
Ama87I	CYCGRG	1 cut(s)	2211
AoxI	GGCC	5 cut(s)	7, 477, 1011, 1702, 1721
ApeKI	GCWGC	4 cut(s)	1180, 2028, 2267, 2435
ApoI	RAATTY	5 cut(s)	292, 627, 688, 1853, 2312
Asp718I	GGTACC	1 cut(s)	1617
AspLEI	GCGC	8 cut(s)	319, 403, 457, 728, 769, 961, 1033, 2203
AspS9I	GGNCC	5 cut(s)	7, 409, 1258, 1721, 2230
AsuC2I	CCSGG	4 cut(s)	511, 721, 2212, 2213
AsuHPI	GGTGA	8 cut(s)	547, 896, 1082, 1199, 1309, 1793, 2294, 2402
AsuII	TTCGAA	2 cut(s)	274, 930
AvaI	CYCGRG	1 cut(s)	2211
AvaII	GGWCC	3 cut(s)	409, 1258, 2230
BaeI	ACNNNNGTAYC	4 cut(s)	1440, 1473, 1959, 1992
BalI	TGGCCA	1 cut(s)	1704
BanI	GGYRCC	2 cut(s)	1433, 1617
BanII	GRGCYC	1 cut(s)	1271
BauI	CACGAG	1 cut(s)	1356
BbsI	GAAGAC	2 cut(s)	1336, 1503
Bbv12I	GWGCWC	1 cut(s)	1271
BbvI	GCAGC	4 cut(s)	1167, 2040, 2279, 2447
BccI	CCATC	3 cut(s)	973, 1274, 1811
BceAI	ACGGC	6 cut(s)	44, 53, 406, 431, 759, 2168
BcgI	CGANNNNNNTGC	2 cut(s)	2330, 2364
BciT130I	CCWGG	4 cut(s)	730, 1529, 2075, 2374
BciVI	GTATCC	1 cut(s)	1441
BcnI	CCSGG	4 cut(s)	511, 721, 2212, 2213
BcoDI	GTCTC	7 cut(s)	175, 593, 797, 1464, 1638, 1773, 1943
BfaI	CTAG	8 cut(s)	549, 675, 879, 1326, 1578, 1872, 2234, 2264
BfmI	CTRYAG	3 cut(s)	1181, 1636, 2055
BfoI	RGCGCY	5 cut(s)	320, 458, 729, 770, 1034
BfrI	CTTAAG	3 cut(s)	905, 1939, 2483
BfuI	GTATCC	1 cut(s)	1441
BglI	GCCNNNNNGGC	2 cut(s)	452, 485
BglII	AGATCT	1 cut(s)	2079
Bme1390I	CCNGG	8 cut(s)	511, 721, 730, 1529, 2075, 2212, 2213, 2374
Bme18I	GGWCC	3 cut(s)	409, 1258, 2230
BmeRI	GACNNNNNGTC	1 cut(s)	1894
BmeT110I	CYCGRG	1 cut(s)	2211
BmgBI	CACGTC	2 cut(s)	91, 484
BmgT120I	GGNCC	5 cut(s)	7, 409, 1258, 1721, 2230
BmiI	GGNNCC	6 cut(s)	697, 718, 1260, 1435, 1619, 1722
BmrFI	CCNGG	8 cut(s)	511, 721, 730, 1529, 2075, 2212, 2213, 2374
BmsI	GCATC	1 cut(s)	2050
BoxI	GACNNNNGTC	2 cut(s)	2136, 2228
BpiI	GAAGAC	2 cut(s)	1336, 1503
BplI	GAGNNNNNCTC	8 cut(s)	201, 233, 1459, 1491, 1461, 1493, 1529, 1561
BpmI	CTGGAG	5 cut(s)	714, 1055, 1437, 2096, 2187
Bpu14I	TTCGAA	2 cut(s)	274, 930
BpuEI	CTTGAG	4 cut(s)	137, 291, 913, 2155
BpuMI	CCSGG	4 cut(s)	511, 721, 2212, 2213
BsaHI	GRCGYC	3 cut(s)	393, 444, 507
BsaJI	CCNNGG	5 cut(s)	432, 510, 1528, 2211, 2372
Bsc4I	CCNNNNNNNGG	6 cut(s)	418, 421, 516, 705, 1614, 1658
Bse1I	ACTGG	3 cut(s)	636, 1454, 2170
Bse3DI	GCAATG	3 cut(s)	878, 1324, 2430
BseBI	CCWGG	4 cut(s)	730, 1529, 2075, 2374
BseDI	CCNNGG	5 cut(s)	432, 510, 1528, 2211, 2372
BseGI	GGATG	6 cut(s)	252, 1604, 1822, 1990, 2257, 2382
BseLI	CCNNNNNNNGG	6 cut(s)	418, 421, 516, 705, 1614, 1658
BseMI	GCAATG	3 cut(s)	878, 1324, 2430
BseMII	CTCAG	3 cut(s)	632, 1263, 2217
BseNI	ACTGG	3 cut(s)	636, 1454, 2170
BseXI	GCAGC	4 cut(s)	1167, 2040, 2279, 2447
BseYI	CCCAGC	1 cut(s)	2438
Bsh1236I	CGCG	2 cut(s)	452, 527
Bsh1285I	CGRYCG	1 cut(s)	504
BshFI	GGCC	5 cut(s)	9, 479, 1013, 1704, 1723
BshNI	GGYRCC	2 cut(s)	1433, 1617
BsiEI	CGRYCG	1 cut(s)	504
BsiHKAI	GWGCWC	1 cut(s)	1271
BsiHKCI	CYCGRG	1 cut(s)	2211
BsiSI	CCGG	4 cut(s)	510, 721, 796, 2212
BsiWI	CGTACG	1 cut(s)	257
BslFI	GGGAC	3 cut(s)	527, 1159, 2001
BslI	CCNNNNNNNGG	6 cut(s)	418, 421, 516, 705, 1614, 1658
BsmAI	GTCTC	7 cut(s)	175, 593, 797, 1464, 1638, 1773, 1943
BsmBI	CGTCTC	1 cut(s)	175
BsmFI	GGGAC	3 cut(s)	527, 1159, 2001
BsmI	GAATGC	2 cut(s)	1096, 2503
BsnI	GGCC	5 cut(s)	9, 479, 1013, 1704, 1723
BsoBI	CYCGRG	1 cut(s)	2211
Bsp119I	TTCGAA	2 cut(s)	274, 930
Bsp1286I	GDGCHC	1 cut(s)	1271
Bsp143I	GATC	4 cut(s)	537, 1516, 2079, 2347
BspANI	GGCC	5 cut(s)	9, 479, 1013, 1704, 1723
BspCNI	CTCAG	3 cut(s)	633, 1264, 2218
BspFNI	CGCG	2 cut(s)	452, 527
BspLI	GGNNCC	6 cut(s)	697, 718, 1260, 1435, 1619, 1722
BspMAI	CTGCAG	1 cut(s)	1185
BspQI	GCTCTTC	1 cut(s)	1259
BspT104I	TTCGAA	2 cut(s)	274, 930
BspT107I	GGYRCC	2 cut(s)	1433, 1617
BspTI	CTTAAG	3 cut(s)	905, 1939, 2483
BsrBI	CCGCTC	1 cut(s)	1984
BsrDI	GCAATG	3 cut(s)	878, 1324, 2430
BsrI	ACTGG	3 cut(s)	636, 1454, 2170
BssECI	CCNNGG	5 cut(s)	432, 510, 1528, 2211, 2372
BssMI	GATC	4 cut(s)	537, 1516, 2079, 2347
BssNI	GRCGYC	3 cut(s)	393, 444, 507
BssSI	CACGAG	1 cut(s)	1356
Bst2BI	CACGAG	1 cut(s)	1356
Bst2UI	CCWGG	4 cut(s)	730, 1529, 2075, 2374
Bst4CI	ACNGT	9 cut(s)	262, 436, 791, 1021, 1153, 1164, 1567, 1670, 2109
Bst6I	CTCTTC	7 cut(s)	109, 336, 927, 933, 1103, 1259, 1636
BstACI	GRCGYC	3 cut(s)	393, 444, 507
BstAFI	CTTAAG	3 cut(s)	905, 1939, 2483
BstBI	TTCGAA	2 cut(s)	274, 930
BstC8I	GCNNGC	6 cut(s)	7, 666, 801, 963, 1702, 2307
BstDEI	CTNAG	7 cut(s)	641, 669, 1272, 1656, 2010, 2226, 2364
BstDSI	CCRYGG	1 cut(s)	432
BstENI	CCTNNNNNAGG	1 cut(s)	1612
BstF5I	GGATG	6 cut(s)	252, 1604, 1822, 1990, 2257, 2382
BstFNI	CGCG	2 cut(s)	452, 527
BstH2I	RGCGCY	5 cut(s)	320, 458, 729, 770, 1034
BstHHI	GCGC	8 cut(s)	319, 403, 457, 728, 769, 961, 1033, 2203
BstKTI	GATC	4 cut(s)	540, 1519, 2082, 2350
BstMAI	GTCTC	7 cut(s)	175, 593, 797, 1464, 1638, 1773, 1943
BstMBI	GATC	4 cut(s)	537, 1516, 2079, 2347
BstMCI	CGRYCG	1 cut(s)	504
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 65, 452, 476, 485, 752, 761
BstNI	CCWGG	4 cut(s)	730, 1529, 2075, 2374
BstNSI	RCATGY	2 cut(s)	668, 965
BstPAI	GACNNNNGTC	2 cut(s)	2136, 2228
BstSCI	CCNGG	8 cut(s)	509, 719, 728, 1527, 2073, 2210, 2211, 2372
BstSFI	CTRYAG	3 cut(s)	1181, 1636, 2055
BstUI	CGCG	2 cut(s)	452, 527
BstV1I	GCAGC	4 cut(s)	1167, 2040, 2279, 2447
BstV2I	GAAGAC	2 cut(s)	1336, 1503
BstX2I	RGATCY	1 cut(s)	2079
BstXI	CCANNNNNNTGG	1 cut(s)	1444
BstYI	RGATCY	1 cut(s)	2079
BsuI	GTATCC	1 cut(s)	1441
BsuRI	GGCC	5 cut(s)	9, 479, 1013, 1704, 1723
BtgI	CCRYGG	1 cut(s)	432
BtrI	CACGTC	2 cut(s)	91, 484
BtsCI	GGATG	6 cut(s)	252, 1604, 1822, 1990, 2257, 2382
BtsI	GCAGTG	4 cut(s)	21, 828, 972, 1547
BtsIMutI	CAGTG	7 cut(s)	21, 828, 972, 1026, 1547, 2063, 2114
Cac8I	GCNNGC	6 cut(s)	7, 666, 801, 963, 1702, 2307
CfoI	GCGC	8 cut(s)	319, 403, 457, 728, 769, 961, 1033, 2203
Cfr13I	GGNCC	5 cut(s)	7, 409, 1258, 1721, 2230
Cfr9I	CCCGGG	1 cut(s)	2211
CseI	GACGC	3 cut(s)	382, 452, 515
Csp6I	GTAC	7 cut(s)	258, 388, 520, 1160, 1353, 1618, 1968
CviAII	CATG	5 cut(s)	665, 962, 1430, 1480, 2502
CviQI	GTAC	7 cut(s)	258, 388, 520, 1160, 1353, 1618, 1968
DdeI	CTNAG	7 cut(s)	641, 669, 1272, 1656, 2010, 2226, 2364
DpnI	GATC	4 cut(s)	539, 1518, 2081, 2349
DpnII	GATC	4 cut(s)	537, 1516, 2079, 2347
DraI	TTTAAA	1 cut(s)	1612
DriI	GACNNNNNGTC	1 cut(s)	1894
EaeI	YGGCCR	1 cut(s)	1702
Eam1104I	CTCTTC	7 cut(s)	109, 336, 927, 933, 1103, 1259, 1636
Eam1105I	GACNNNNNGTC	1 cut(s)	1894
EarI	CTCTTC	7 cut(s)	109, 336, 927, 933, 1103, 1259, 1636
EciI	GGCGGA	3 cut(s)	46, 97, 127
Ecl136II	GAGCTC	1 cut(s)	1269
Eco24I	GRGCYC	1 cut(s)	1271
Eco47I	GGWCC	3 cut(s)	409, 1258, 2230
Eco53kI	GAGCTC	1 cut(s)	1269
Eco57I	CTGAAG	1 cut(s)	624
Eco88I	CYCGRG	1 cut(s)	2211
EcoICRI	GAGCTC	1 cut(s)	1269
EcoNI	CCTNNNNNAGG	1 cut(s)	1612
EcoO109I	RGGNCCY	1 cut(s)	2230
EcoRI	GAATTC	2 cut(s)	688, 2312
EcoRII	CCWGG	4 cut(s)	728, 1527, 2073, 2372
EcoT22I	ATGCAT	1 cut(s)	2503
EcoT38I	GRGCYC	1 cut(s)	1271
Esp3I	CGTCTC	1 cut(s)	175
FaeI	CATG	5 cut(s)	668, 965, 1433, 1483, 2505
FaqI	GGGAC	3 cut(s)	527, 1159, 2001
FatI	CATG	5 cut(s)	664, 961, 1429, 1479, 2501
FauI	CCCGC	2 cut(s)	481, 1977
FblI	GTMKAC	3 cut(s)	63, 504, 1635
FokI	GGATG	6 cut(s)	239, 1591, 1829, 1977, 2264, 2389
FriOI	GRGCYC	1 cut(s)	1271
FspBI	CTAG	8 cut(s)	549, 675, 879, 1326, 1578, 1872, 2234, 2264
FspI	TGCGCA	2 cut(s)	960, 2202
GlaI	GCGC	8 cut(s)	318, 402, 456, 727, 768, 960, 1032, 2202
GsaI	CCCAGC	1 cut(s)	2442
GsuI	CTGGAG	5 cut(s)	714, 1055, 1437, 2096, 2187
HaeII	RGCGCY	5 cut(s)	320, 458, 729, 770, 1034
HaeIII	GGCC	5 cut(s)	9, 479, 1013, 1704, 1723
HapII	CCGG	4 cut(s)	510, 721, 796, 2212
HgaI	GACGC	3 cut(s)	382, 452, 515
HhaI	GCGC	8 cut(s)	319, 403, 457, 728, 769, 961, 1033, 2203
Hin1I	GRCGYC	3 cut(s)	393, 444, 507
Hin1II	CATG	5 cut(s)	668, 965, 1433, 1483, 2505
Hin6I	GCGC	8 cut(s)	317, 401, 455, 726, 767, 959, 1031, 2201
HinP1I	GCGC	8 cut(s)	317, 401, 455, 726, 767, 959, 1031, 2201
HincII	GTYRAC	1 cut(s)	505
HindII	GTYRAC	1 cut(s)	505
HindIII	AAGCTT	3 cut(s)	889, 1340, 2012
HpaII	CCGG	4 cut(s)	510, 721, 796, 2212
HphI	GGTGA	8 cut(s)	547, 896, 1082, 1199, 1309, 1793, 2294, 2402
Hpy166II	GTNNAC	4 cut(s)	64, 432, 505, 1636
Hpy8I	GTNNAC	4 cut(s)	64, 432, 505, 1636
Hpy99I	CGWCG	4 cut(s)	74, 452, 485, 509
HpyCH4III	ACNGT	9 cut(s)	262, 436, 791, 1021, 1153, 1164, 1567, 1670, 2109
HpyCH4IV	ACGT	2 cut(s)	90, 483
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 65, 452, 476, 485, 752, 761
HpyF3I	CTNAG	7 cut(s)	641, 669, 1272, 1656, 2010, 2226, 2364
HpySE526I	ACGT	2 cut(s)	90, 483
Hsp92I	GRCGYC	3 cut(s)	393, 444, 507
Hsp92II	CATG	5 cut(s)	668, 965, 1433, 1483, 2505
HspAI	GCGC	8 cut(s)	317, 401, 455, 726, 767, 959, 1031, 2201
KpnI	GGTACC	1 cut(s)	1621
Kzo9I	GATC	4 cut(s)	537, 1516, 2079, 2347
LguI	GCTCTTC	1 cut(s)	1259
LmnI	GCTCC	8 cut(s)	243, 305, 682, 695, 723, 982, 1040, 1240
Lsp1109I	GCAGC	4 cut(s)	1167, 2040, 2279, 2447
LweI	GCATC	1 cut(s)	2050
MaeI	CTAG	8 cut(s)	549, 675, 879, 1326, 1578, 1872, 2234, 2264
MaeII	ACGT	2 cut(s)	90, 483
MaeIII	GTNAC	5 cut(s)	91, 1000, 1358, 2103, 2332
MalI	GATC	4 cut(s)	539, 1518, 2081, 2349
MbiI	CCGCTC	1 cut(s)	1984
MboI	GATC	4 cut(s)	537, 1516, 2079, 2347
MflI	RGATCY	1 cut(s)	2079
MhlI	GDGCHC	1 cut(s)	1271
MlsI	TGGCCA	1 cut(s)	1704
MluNI	TGGCCA	1 cut(s)	1704
MlyI	GAGTC	6 cut(s)	95, 114, 1326, 1493, 2146, 2178
MmeI	TCCRAC	7 cut(s)	7, 339, 714, 789, 1203, 1794, 1806
Mox20I	TGGCCA	1 cut(s)	1704
Mph1103I	ATGCAT	1 cut(s)	2503
MscI	TGGCCA	1 cut(s)	1704
MslI	CAYNNNNRTG	2 cut(s)	1019, 1478
Msp20I	TGGCCA	1 cut(s)	1704
MspA1I	CMGCKG	1 cut(s)	2438
MspCI	CTTAAG	3 cut(s)	905, 1939, 2483
MspI	CCGG	4 cut(s)	510, 721, 796, 2212
MspR9I	CCNGG	8 cut(s)	511, 721, 730, 1529, 2075, 2212, 2213, 2374
Mva1269I	GAATGC	2 cut(s)	1096, 2503
MvaI	CCWGG	4 cut(s)	730, 1529, 2075, 2374
MvnI	CGCG	2 cut(s)	452, 527
MwoI	GCNNNNNNNGC	7 cut(s)	11, 65, 452, 476, 485, 752, 761
NciI	CCSGG	4 cut(s)	511, 721, 2212, 2213
NdeII	GATC	4 cut(s)	537, 1516, 2079, 2347
NlaIII	CATG	5 cut(s)	668, 965, 1433, 1483, 2505
NlaIV	GGNNCC	6 cut(s)	697, 718, 1260, 1435, 1619, 1722
NmuCI	GTSAC	5 cut(s)	91, 1000, 1358, 2103, 2332
NsbI	TGCGCA	2 cut(s)	960, 2202
NsiI	ATGCAT	1 cut(s)	2503
NspI	RCATGY	2 cut(s)	668, 965
NspV	TTCGAA	2 cut(s)	274, 930
PaeI	GCATGC	2 cut(s)	668, 965
PciSI	GCTCTTC	1 cut(s)	1259
PcsI	WCGNNNNNNNCGW	4 cut(s)	96, 327, 336, 620
PctI	GAATGC	2 cut(s)	1096, 2503
PfeI	GAWTC	6 cut(s)	203, 276, 374, 619, 2360, 2368
Pfl23II	CGTACG	1 cut(s)	257
PflFI	GACNNNGTC	1 cut(s)	446
PfoI	TCCNGGA	1 cut(s)	719
PleI	GAGTC	6 cut(s)	95, 114, 1326, 1493, 2145, 2177
PpsI	GAGTC	6 cut(s)	95, 114, 1326, 1493, 2145, 2177
PpuMI	RGGWCCY	1 cut(s)	2230
PshAI	GACNNNNGTC	2 cut(s)	2136, 2228
Psp124BI	GAGCTC	1 cut(s)	1271
Psp5II	RGGWCCY	1 cut(s)	2230
Psp6I	CCWGG	4 cut(s)	728, 1527, 2073, 2372
PspFI	CCCAGC	1 cut(s)	2438
PspGI	CCWGG	4 cut(s)	728, 1527, 2073, 2372
PspLI	CGTACG	1 cut(s)	257
PspN4I	GGNNCC	6 cut(s)	697, 718, 1260, 1435, 1619, 1722
PspPI	GGNCC	5 cut(s)	7, 409, 1258, 1721, 2230
PspPPI	RGGWCCY	1 cut(s)	2230
PstI	CTGCAG	1 cut(s)	1185
PsuI	RGATCY	1 cut(s)	2079
PsyI	GACNNNGTC	1 cut(s)	446
PvuII	CAGCTG	1 cut(s)	2438
RsaI	GTAC	7 cut(s)	259, 389, 521, 1161, 1354, 1619, 1969
RsaNI	GTAC	7 cut(s)	258, 388, 520, 1160, 1353, 1618, 1968
RseI	CAYNNNNRTG	2 cut(s)	1019, 1478
SacI	GAGCTC	1 cut(s)	1271
SalI	GTCGAC	1 cut(s)	503
SapI	GCTCTTC	1 cut(s)	1259
Sau3AI	GATC	4 cut(s)	537, 1516, 2079, 2347
Sau96I	GGNCC	5 cut(s)	7, 409, 1258, 1721, 2230
SchI	GAGTC	6 cut(s)	95, 114, 1326, 1493, 2146, 2178
ScrFI	CCNGG	8 cut(s)	511, 721, 730, 1529, 2075, 2212, 2213, 2374
SduI	GDGCHC	1 cut(s)	1271
SfaNI	GCATC	1 cut(s)	2050
SfcI	CTRYAG	3 cut(s)	1181, 1636, 2055
SfuI	TTCGAA	2 cut(s)	274, 930
SinI	GGWCC	3 cut(s)	409, 1258, 2230
SmaI	CCCGGG	1 cut(s)	2213
SmiMI	CAYNNNNRTG	2 cut(s)	1019, 1478
SmlI	CTYRAG	7 cut(s)	152, 306, 892, 905, 1939, 2134, 2483
SmoI	CTYRAG	7 cut(s)	152, 306, 892, 905, 1939, 2134, 2483
SphI	GCATGC	2 cut(s)	668, 965
SspI	AATATT	1 cut(s)	1774
SspMI	CTAG	8 cut(s)	549, 675, 879, 1326, 1578, 1872, 2234, 2264
SstI	GAGCTC	1 cut(s)	1271
StyD4I	CCNGG	8 cut(s)	509, 719, 728, 1527, 2073, 2210, 2211, 2372
TaaI	ACNGT	9 cut(s)	262, 436, 791, 1021, 1153, 1164, 1567, 1670, 2109
TaiI	ACGT	2 cut(s)	93, 486
TaqII	GACCGA	2 cut(s)	353, 490
TauI	GCSGC	9 cut(s)	143, 146, 284, 287, 425, 455, 527, 764, 767
TfiI	GAWTC	6 cut(s)	203, 276, 374, 619, 2360, 2368
TscAI	CASTG	7 cut(s)	21, 828, 972, 1026, 1554, 2070, 2114
TseFI	GTSAC	5 cut(s)	91, 1000, 1358, 2103, 2332
TseI	GCWGC	4 cut(s)	1180, 2028, 2267, 2435
Tsp45I	GTSAC	5 cut(s)	91, 1000, 1358, 2103, 2332
TspDTI	ATGAA	5 cut(s)	1335, 1607, 2040, 2193, 2421
TspGWI	ACGGA	2 cut(s)	184, 245
TspMI	CCCGGG	1 cut(s)	2211
TspRI	CASTG	7 cut(s)	21, 828, 972, 1026, 1554, 2070, 2114
Tth111I	GACNNNGTC	1 cut(s)	446
Vha464I	CTTAAG	3 cut(s)	905, 1939, 2483
VpaK11BI	GGWCC	3 cut(s)	409, 1258, 2230
XagI	CCTNNNNNAGG	1 cut(s)	1612
XapI	RAATTY	5 cut(s)	292, 627, 688, 1853, 2312
XceI	RCATGY	2 cut(s)	668, 965
XcmI	CCANNNNNNNNNTGG	1 cut(s)	310
XmaI	CCCGGG	1 cut(s)	2211
XmiI	GTMKAC	3 cut(s)	63, 504, 1635
XspI	CTAG	8 cut(s)	549, 675, 879, 1326, 1578, 1872, 2234, 2264
Zsp2I	ATGCAT	1 cut(s)	2503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.