Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009653

Physical Location & Seq

Genomic Coordinates Forward (+)

2042011 .. 2048552

Gene Structure 6542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1010000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGCGAGCCCTCTGACACCATCGCATGTTGTTATCGCCTATGACGCCACCAAGGACCGCGGCGTGCATGAGCTTACGCTCGCAATTGACAGTCTCCAGAAGAGGGGTGACGTTTTACATTCAGGGGATACCATCATTGTGCTTGGAGTTTTGCATAAGGTTGTCCACCCGATGGGATATAACACAAAACCATGCGTGGATTTTTTGGAAAACAGCATTCGTGCAGTGGAGGAAGAAGTTACAAAGAAAGTTGACATGTATGTTAACATGCTTCTTCAAAGTGCGGGAAAGTGTGAAGATCAAGGGGTCAGCATTGAAGTCAAAGTTACTGCTGGTTTTCCCGTTAAGCAGGTTATTTTGCAAGAGATTATGGCCTGCAATGCAGCTTGGGTTGTACTTGATAGGCACCTTAGGCGGGATTTGACGTTCTACCTGAAGCAGATACCGTGCAAGGTTGCAATAATTCAAGATAGCTTGGCTGTGGAAGTTGTGAGACCTCATACAACGGATGAAACTGACACTATAGAACACAAATTGTTTTTTTCTTTGTCCAAGCCCGTTCCTCTATCCAATCAAGGTAACGAAAACAATGAACCACTCAGCACTTGCGGAAGCTGCTTTGTATCCATTGATTCACCAGAAAGCTCTGAGATTCTGAAAAATCACTTTGTAACTTCACCTACATTTAAGTTACGAGACTACGGTACTTTATTGAATCTAGGGTCTTCCTCTAAGCAAGAAAATTCAGGTGTTCAAACCAAAGCAGACAATAAACCATCCACTGTTCCTCAAAAAATCCAGAAGCAAAATAACGCCTTCCGAGAAAGATGCTCAGATGCCCCAATTCTGTGTTCCAGATGCGGAACAAGGACTGAAATGTATGTCAAAGATTCCATGACATTCAGCTACTCAGAGATACAGCTTGCAACAAATGATTTTTCCAAGGAAAACTTATTAGGAGAAGGTGGATATGGTCATGTGTATAAAGGTGAGCTCAAGGACGGACAGCAGATTGCAGCAAAAGTGCGAAAAGAAGCAAGTACACAAGGATTTAAGGAATTTCACTCTGAGGTTTATGTCTTGAGTTTTGCACGCCATAAGAACATTGTCATGCTGTTGGGTTATTGTTGCAAGGAGAACCTAAATATCTTGGTTTATGAATATATTTGCCACAATTCTCTTGAATGGCACTTATTTGATGAAAAAGCAGCAGCTCTTGAGTGGCACCAAAGACATTCTATTGCCATTGGAACTGCAAAAGGATTGCGTTTCCTACACGAAGAATGTCGTGGAGGTCCTATAATTCATAGAGACACGCGTCCAAGCAATATCCTGCTAACACATGATTATGTTCCTATGTTAGGTGATTTTGGCTTAGCCAAATGGAAGACAACGGGTGATGACACTGACCCGACAAGGATAATTGGCACACTAGGCTACCTTGCTCCAGAGTATGCGGAGAACGGTATTGTTTCTGTACGGACAGATGTATATGCCTTTGGTATGGTTCTGTTACAACTAATAACCGGCCGCAAGATTATTGATTCAAATGGAGAAGGGAAAGTTCAATCCCTACGGGAGTGGGCAGAACCAATAATCCAGAGGCTAGCACTACATGAACTCATTGATCTTCGTTTAGGAGATTCGTATGAGACATATGGAGTGTACCTTATGGCTAAAGCCGCATACTTATGCATACAAAGAAGTCCTGAAATGCGTCCATCAATGGGAGAGGTTGTCCGTGTTCTTGAAGGTGAAAGTGACAATTTCCACCACCTAGGAGTGCGCTTTGTACCTGAACAGCATACAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

605

Amino Acids

67.73

Weight (kDa)

6.17

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	314 - 578	2.1e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	316 - 529	1.2e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009653)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g15260
malus_domestica	MD05G1010000.v1.1 MD10G1008700.v1.1
prunus_persica	Prupe.8G012200_v2.0.a1 Prupe.8G012300_v2.0.a1 Prupe.8G012500_v2.0.a1
pyrus_communis	pycom05g00520 pycom10g00520
rosa_chinensis	RchiOBHm_Chr6g0277981
rosa_laevigata	RLG00000013250
rosa_multiflora	Rmu_sc0001474.1_g000022
rosa_roxburghii	Rroxscaffold_7G00190610 Rroxscaffold_7G00190620
rosa_rugosa	Rorug06G0113500 Rorug06G0113600 Rorug06G0113700 Rorug06G0113800
rosa_samantha	Rh6AG223500 Rh6BG227700 Rh6CG230200 Rh6DG221000
rosa_wichuraiana	Rw6G019530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	340
AccB1I	GGYRCC	2 cut(s)	405, 1224
AccB7I	CCANNNNNTGG	1 cut(s)	172
AccII	CGCG	2 cut(s)	60, 1318
AciI	CCGC	9 cut(s)	58, 60, 284, 415, 609, 861, 1457, 1531, 1683
AcoI	YGGCCR	1 cut(s)	1528
AcsI	RAATTY	2 cut(s)	742, 1058
AcuI	CTGAAG	1 cut(s)	455
AcyI	GRCGYC	1 cut(s)	45
AfaI	GTAC	6 cut(s)	396, 706, 1042, 1479, 1667, 1794
AfiI	CCNNNNNNNGG	5 cut(s)	103, 172, 415, 1361, 1727
AflIII	ACRYGT	2 cut(s)	255, 1316
AgsI	TTSAA	9 cut(s)	278, 317, 467, 715, 755, 1184, 1548, 1568, 1751
AjuI	GAANNNNNNNTTGG	2 cut(s)	835, 867
AloI	GAACNNNNNNTCC	4 cut(s)	410, 442, 1581, 1613
AluBI	AGCT	9 cut(s)	73, 386, 474, 615, 645, 906, 922, 994, 1214
AluI	AGCT	9 cut(s)	73, 386, 474, 615, 645, 906, 922, 994, 1214
Alw21I	GWGCWC	1 cut(s)	996
Alw26I	GTCTC	5 cut(s)	98, 487, 690, 1305, 1646
AoxI	GGCC	2 cut(s)	372, 1528
ApeKI	GCWGC	5 cut(s)	383, 615, 1016, 1208, 1211
ApoI	RAATTY	2 cut(s)	742, 1058
AspA2I	CCTAGG	1 cut(s)	1777
AspLEI	GCGC	1 cut(s)	1788
AspS9I	GGNCC	2 cut(s)	55, 1295
AsuHPI	GGTGA	7 cut(s)	119, 627, 669, 1001, 1376, 1409, 1766
AsuNHI	GCTAGC	1 cut(s)	1606
AvaII	GGWCC	2 cut(s)	55, 1295
AvrII	CCTAGG	1 cut(s)	1777
AxyI	CCTNAGG	1 cut(s)	410
BanI	GGYRCC	2 cut(s)	405, 1224
BanII	GRGCYC	2 cut(s)	11, 996
BbsI	GAAGAC	2 cut(s)	717, 1394
Bbv12I	GWGCWC	1 cut(s)	996
BbvI	GCAGC	5 cut(s)	395, 602, 1028, 1220, 1223
BccI	CCATC	5 cut(s)	28, 140, 166, 784, 1729
BcgI	CGANNNNNNTGC	2 cut(s)	810, 844
BciVI	GTATCC	2 cut(s)	121, 634
BcoDI	GTCTC	5 cut(s)	98, 487, 690, 1305, 1646
BfaI	CTAG	4 cut(s)	719, 1433, 1607, 1778
BfmI	CTRYAG	1 cut(s)	522
BfuAI	ACCTGC	1 cut(s)	340
BfuI	GTATCC	2 cut(s)	121, 634
BisI	GCNGC	8 cut(s)	61, 384, 616, 1017, 1209, 1212, 1531, 1683
BlnI	CCTAGG	1 cut(s)	1777
BlpI	GCTNAGC	1 cut(s)	1375
BlsI	GCNGC	8 cut(s)	62, 385, 617, 1018, 1210, 1213, 1532, 1684
Bme18I	GGWCC	2 cut(s)	55, 1295
BmgT120I	GGNCC	2 cut(s)	55, 1295
BmiI	GGNNCC	2 cut(s)	407, 1226
BmsI	GCATC	3 cut(s)	818, 826, 848
BmtI	GCTAGC	1 cut(s)	1610
BoxI	GACNNNNGTC	1 cut(s)	1317
BpiI	GAAGAC	2 cut(s)	717, 1394
BpmI	CTGGAG	2 cut(s)	80, 1431
Bpu1102I	GCTNAGC	1 cut(s)	1375
BpuEI	CTTGAG	3 cut(s)	980, 1102, 1238
BsaHI	GRCGYC	1 cut(s)	45
BsaI	GGTCTC	1 cut(s)	487
BsaJI	CCNNGG	4 cut(s)	51, 58, 942, 1777
BsaXI	ACNNNNNCTCC	2 cut(s)	1722, 1752
Bsc4I	CCNNNNNNNGG	5 cut(s)	103, 172, 415, 1361, 1727
Bse118I	RCCGGY	1 cut(s)	1526
Bse21I	CCTNAGG	1 cut(s)	410
Bse3DI	GCAATG	1 cut(s)	385
BseDI	CCNNGG	4 cut(s)	51, 58, 942, 1777
BseGI	GGATG	2 cut(s)	514, 776
BseLI	CCNNNNNNNGG	5 cut(s)	103, 172, 415, 1361, 1727
BseMI	GCAATG	1 cut(s)	385
BseMII	CTCAG	5 cut(s)	613, 639, 846, 924, 1059
BseX3I	CGGCCG	1 cut(s)	1528
BseXI	GCAGC	5 cut(s)	395, 602, 1028, 1220, 1223
BsgI	GTGCAG	1 cut(s)	243
Bsh1236I	CGCG	2 cut(s)	60, 1318
Bsh1285I	CGRYCG	1 cut(s)	1531
BshFI	GGCC	2 cut(s)	374, 1530
BshNI	GGYRCC	2 cut(s)	405, 1224
BsiEI	CGRYCG	1 cut(s)	1531
BsiHKAI	GWGCWC	1 cut(s)	996
BsiSI	CCGG	1 cut(s)	1527
BslI	CCNNNNNNNGG	5 cut(s)	103, 172, 415, 1361, 1727
BsmAI	GTCTC	5 cut(s)	98, 487, 690, 1305, 1646
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	2 cut(s)	374, 1530
Bso31I	GGTCTC	1 cut(s)	487
Bsp1286I	GDGCHC	2 cut(s)	11, 996
Bsp143I	GATC	2 cut(s)	298, 1627
Bsp1720I	GCTNAGC	1 cut(s)	1375
BspACI	CCGC	9 cut(s)	58, 60, 284, 415, 609, 861, 1457, 1531, 1683
BspANI	GGCC	2 cut(s)	374, 1530
BspCNI	CTCAG	5 cut(s)	612, 640, 845, 923, 1060
BspFNI	CGCG	2 cut(s)	60, 1318
BspLI	GGNNCC	2 cut(s)	407, 1226
BspMI	ACCTGC	1 cut(s)	340
BspOI	GCTAGC	1 cut(s)	1610
BspT107I	GGYRCC	2 cut(s)	405, 1224
BspTNI	GGTCTC	1 cut(s)	487
BsrDI	GCAATG	1 cut(s)	385
BsrFI	RCCGGY	1 cut(s)	1526
BssAI	RCCGGY	1 cut(s)	1526
BssECI	CCNNGG	4 cut(s)	51, 58, 942, 1777
BssMI	GATC	2 cut(s)	298, 1627
BssNI	GRCGYC	1 cut(s)	45
BssT1I	CCWWGG	3 cut(s)	51, 942, 1777
Bst4CI	ACNGT	5 cut(s)	92, 447, 704, 784, 1466
Bst6I	CTCTTC	1 cut(s)	95
BstACI	GRCGYC	1 cut(s)	45
BstC8I	GCNNGC	7 cut(s)	7, 65, 81, 376, 924, 1093, 1608
BstDEI	CTNAG	8 cut(s)	410, 599, 648, 732, 832, 910, 1068, 1375
BstDSI	CCRYGG	1 cut(s)	58
BstENI	CCTNNNNNAGG	1 cut(s)	1359
BstF5I	GGATG	2 cut(s)	514, 776
BstFNI	CGCG	2 cut(s)	60, 1318
BstHHI	GCGC	1 cut(s)	1788
BstKTI	GATC	2 cut(s)	301, 1630
BstMAI	GTCTC	5 cut(s)	98, 487, 690, 1305, 1646
BstMBI	GATC	2 cut(s)	298, 1627
BstMCI	CGRYCG	1 cut(s)	1531
BstMWI	GCNNNNNNNGC	6 cut(s)	44, 380, 412, 615, 1434, 1682
BstNSI	RCATGY	3 cut(s)	29, 259, 271
BstPAI	GACNNNNGTC	1 cut(s)	1317
BstSFI	CTRYAG	1 cut(s)	522
BstUI	CGCG	2 cut(s)	60, 1318
BstV1I	GCAGC	5 cut(s)	395, 602, 1028, 1220, 1223
BstV2I	GAAGAC	2 cut(s)	717, 1394
BstZI	CGGCCG	1 cut(s)	1528
Bsu36I	CCTNAGG	1 cut(s)	410
BsuI	GTATCC	2 cut(s)	121, 634
BsuRI	GGCC	2 cut(s)	374, 1530
BtgI	CCRYGG	1 cut(s)	58
BtgZI	GCGATG	1 cut(s)	6
BtsCI	GGATG	2 cut(s)	514, 776
BtsI	GCAGTG	1 cut(s)	231
BtsIMutI	CAGTG	3 cut(s)	231, 780, 1404
BveI	ACCTGC	1 cut(s)	340
Cac8I	GCNNGC	7 cut(s)	7, 65, 81, 376, 924, 1093, 1608
CfoI	GCGC	1 cut(s)	1788
Cfr10I	RCCGGY	1 cut(s)	1526
Cfr13I	GGNCC	2 cut(s)	55, 1295
Cfr42I	CCGCGG	1 cut(s)	61
CseI	GACGC	3 cut(s)	53, 1307, 1706
Csp6I	GTAC	6 cut(s)	395, 705, 1041, 1478, 1666, 1793
CviQI	GTAC	6 cut(s)	395, 705, 1041, 1478, 1666, 1793
DdeI	CTNAG	8 cut(s)	410, 599, 648, 732, 832, 910, 1068, 1375
DpnI	GATC	2 cut(s)	300, 1629
DpnII	GATC	2 cut(s)	298, 1627
EaeI	YGGCCR	1 cut(s)	1528
EagI	CGGCCG	1 cut(s)	1528
Eam1104I	CTCTTC	1 cut(s)	95
EarI	CTCTTC	1 cut(s)	95
Ecl136II	GAGCTC	1 cut(s)	994
EclXI	CGGCCG	1 cut(s)	1528
Eco130I	CCWWGG	3 cut(s)	51, 942, 1777
Eco24I	GRGCYC	2 cut(s)	11, 996
Eco31I	GGTCTC	1 cut(s)	487
Eco47I	GGWCC	2 cut(s)	55, 1295
Eco52I	CGGCCG	1 cut(s)	1528
Eco53kI	GAGCTC	1 cut(s)	994
Eco57I	CTGAAG	1 cut(s)	455
Eco81I	CCTNAGG	1 cut(s)	410
EcoICRI	GAGCTC	1 cut(s)	994
EcoNI	CCTNNNNNAGG	1 cut(s)	1359
EcoO109I	RGGNCCY	1 cut(s)	1295
EcoT14I	CCWWGG	3 cut(s)	51, 942, 1777
EcoT22I	ATGCAT	1 cut(s)	1697
EcoT38I	GRGCYC	2 cut(s)	11, 996
ErhI	CCWWGG	3 cut(s)	51, 942, 1777
FalI	AAGNNNNNCTT	2 cut(s)	935, 967
FauI	CCCGC	2 cut(s)	277, 408
FauNDI	CATATG	1 cut(s)	1657
Fnu4HI	GCNGC	8 cut(s)	61, 384, 616, 1017, 1209, 1212, 1531, 1683
FokI	GGATG	2 cut(s)	521, 763
FriOI	GRGCYC	2 cut(s)	11, 996
Fsp4HI	GCNGC	8 cut(s)	61, 384, 616, 1017, 1209, 1212, 1531, 1683
FspBI	CTAG	4 cut(s)	719, 1433, 1607, 1778
GlaI	GCGC	1 cut(s)	1787
GluI	GCNGC	8 cut(s)	61, 384, 616, 1017, 1209, 1212, 1531, 1683
GsuI	CTGGAG	2 cut(s)	80, 1431
HaeIII	GGCC	2 cut(s)	374, 1530
HapII	CCGG	1 cut(s)	1527
HgaI	GACGC	3 cut(s)	53, 1307, 1706
HhaI	GCGC	1 cut(s)	1788
Hin1I	GRCGYC	1 cut(s)	45
Hin6I	GCGC	1 cut(s)	1786
HinP1I	GCGC	1 cut(s)	1786
HincII	GTYRAC	2 cut(s)	253, 265
HindII	GTYRAC	2 cut(s)	253, 265
HinfI	GANTC	6 cut(s)	632, 652, 715, 890, 1544, 1643
HpaI	GTTAAC	1 cut(s)	265
HpaII	CCGG	1 cut(s)	1527
HphI	GGTGA	7 cut(s)	119, 627, 669, 1001, 1376, 1409, 1766
Hpy166II	GTNNAC	5 cut(s)	166, 253, 265, 1043, 1666
Hpy188I	TCNGA	7 cut(s)	15, 649, 657, 821, 835, 913, 1069
Hpy8I	GTNNAC	5 cut(s)	166, 253, 265, 1043, 1666
HpyAV	CCTTC	4 cut(s)	826, 956, 1550, 1745
HpyCH4III	ACNGT	5 cut(s)	92, 447, 704, 784, 1466
HpyCH4IV	ACGT	2 cut(s)	111, 425
HpyF10VI	GCNNNNNNNGC	6 cut(s)	44, 380, 412, 615, 1434, 1682
HpyF3I	CTNAG	8 cut(s)	410, 599, 648, 732, 832, 910, 1068, 1375
HpySE526I	ACGT	2 cut(s)	111, 425
Hsp92I	GRCGYC	1 cut(s)	45
HspAI	GCGC	1 cut(s)	1786
KspAI	GTTAAC	1 cut(s)	265
KspI	CCGCGG	1 cut(s)	61
Kzo9I	GATC	2 cut(s)	298, 1627
LmnI	GCTCC	1 cut(s)	1450
Lsp1109I	GCAGC	5 cut(s)	395, 602, 1028, 1220, 1223
LweI	GCATC	3 cut(s)	818, 826, 848
MaeI	CTAG	4 cut(s)	719, 1433, 1607, 1778
MaeII	ACGT	2 cut(s)	111, 425
MaeIII	GTNAC	8 cut(s)	107, 238, 325, 578, 670, 690, 1512, 1760
MalI	GATC	2 cut(s)	300, 1629
MboI	GATC	2 cut(s)	298, 1627
MboII	GAAGA	8 cut(s)	112, 245, 266, 308, 717, 1292, 1399, 1622
MfeI	CAATTG	1 cut(s)	84
MhlI	GDGCHC	2 cut(s)	11, 996
MluI	ACGCGT	1 cut(s)	1316
Mph1103I	ATGCAT	1 cut(s)	1697
MseI	TTAA	4 cut(s)	264, 345, 687, 1053
MslI	CAYNNNNRTG	4 cut(s)	137, 1109, 1347, 1690
MspA1I	CMGCKG	1 cut(s)	60
MspI	CCGG	1 cut(s)	1527
MunI	CAATTG	1 cut(s)	84
Mva1269I	GAATGC	1 cut(s)	216
MvnI	CGCG	2 cut(s)	60, 1318
MwoI	GCNNNNNNNGC	6 cut(s)	44, 380, 412, 615, 1434, 1682
NdeI	CATATG	1 cut(s)	1657
NdeII	GATC	2 cut(s)	298, 1627
NheI	GCTAGC	1 cut(s)	1606
NlaIV	GGNNCC	2 cut(s)	407, 1226
NmuCI	GTSAC	2 cut(s)	107, 1760
NsiI	ATGCAT	1 cut(s)	1697
NspI	RCATGY	3 cut(s)	29, 259, 271
PciI	ACATGT	1 cut(s)	255
PctI	GAATGC	1 cut(s)	216
PfeI	GAWTC	6 cut(s)	632, 652, 715, 890, 1544, 1643
PflMI	CCANNNNNTGG	1 cut(s)	172
PkrI	GCNGC	8 cut(s)	62, 385, 617, 1018, 1210, 1213, 1532, 1684
PpuMI	RGGWCCY	1 cut(s)	1295
PscI	ACATGT	1 cut(s)	255
PshAI	GACNNNNGTC	1 cut(s)	1317
Psp124BI	GAGCTC	1 cut(s)	996
Psp5II	RGGWCCY	1 cut(s)	1295
PspN4I	GGNNCC	2 cut(s)	407, 1226
PspPI	GGNCC	2 cut(s)	55, 1295
PspPPI	RGGWCCY	1 cut(s)	1295
RsaI	GTAC	6 cut(s)	396, 706, 1042, 1479, 1667, 1794
RsaNI	GTAC	6 cut(s)	395, 705, 1041, 1478, 1666, 1793
RseI	CAYNNNNRTG	4 cut(s)	137, 1109, 1347, 1690
SacI	GAGCTC	1 cut(s)	996
SacII	CCGCGG	1 cut(s)	61
SaqAI	TTAA	4 cut(s)	264, 345, 687, 1053
SatI	GCNGC	8 cut(s)	61, 384, 616, 1017, 1209, 1212, 1531, 1683
Sau3AI	GATC	2 cut(s)	298, 1627
Sau96I	GGNCC	2 cut(s)	55, 1295
SduI	GDGCHC	2 cut(s)	11, 996
SfaNI	GCATC	3 cut(s)	818, 826, 848
SfcI	CTRYAG	1 cut(s)	522
Sfr303I	CCGCGG	1 cut(s)	61
SgrBI	CCGCGG	1 cut(s)	61
SinI	GGWCC	2 cut(s)	55, 1295
SmiMI	CAYNNNNRTG	4 cut(s)	137, 1109, 1347, 1690
SmlI	CTYRAG	3 cut(s)	995, 1081, 1217
SmoI	CTYRAG	3 cut(s)	995, 1081, 1217
SsiI	CCGC	9 cut(s)	58, 60, 284, 415, 609, 861, 1457, 1531, 1683
SspMI	CTAG	4 cut(s)	719, 1433, 1607, 1778
SstI	GAGCTC	1 cut(s)	996
StyI	CCWWGG	3 cut(s)	51, 942, 1777
TaaI	ACNGT	5 cut(s)	92, 447, 704, 784, 1466
TaiI	ACGT	2 cut(s)	114, 428
TatI	WGTACW	2 cut(s)	394, 1040
TauI	GCSGC	3 cut(s)	63, 1533, 1685
TfiI	GAWTC	6 cut(s)	632, 652, 715, 890, 1544, 1643
Tru1I	TTAA	4 cut(s)	264, 345, 687, 1053
Tru9I	TTAA	4 cut(s)	264, 345, 687, 1053
TscAI	CASTG	3 cut(s)	231, 787, 1411
TseFI	GTSAC	2 cut(s)	107, 1760
TseI	GCWGC	5 cut(s)	383, 615, 1016, 1208, 1211
Tsp45I	GTSAC	2 cut(s)	107, 1760
TspDTI	ATGAA	6 cut(s)	525, 606, 1173, 1215, 1295, 1632
TspGWI	ACGGA	4 cut(s)	521, 1017, 1495, 1730
TspRI	CASTG	3 cut(s)	231, 787, 1411
Van91I	CCANNNNNTGG	1 cut(s)	172
VpaK11BI	GGWCC	2 cut(s)	55, 1295
XagI	CCTNNNNNAGG	1 cut(s)	1359
XapI	RAATTY	2 cut(s)	742, 1058
XceI	RCATGY	3 cut(s)	29, 259, 271
XmaJI	CCTAGG	1 cut(s)	1777
XspI	CTAG	4 cut(s)	719, 1433, 1607, 1778
Zsp2I	ATGCAT	1 cut(s)	1697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.