Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007971

Physical Location & Seq

Genomic Coordinates Forward (+)

6971799 .. 6976636

Gene Structure 4838 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1041400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGCTTATTTCACTCAGAAATCTAGCCAAATCCAAGGCGAATACCAACTGCTCCCTCTGCTGCTCCTCCGCCGCCATCCTCGATGCCAATTTTCTTCTCACCACTACCGCCGCCGCTGCTGCCACCAGAACCAGCAGTTCCTTCAGTTTCAGCCCCAACGCTTCCCCTCCCCCCGCTCCTTTTTCAACCTCAGCTCGCTTGACTCCGGCAAGGGACGTGGTCCTCTCTTTCAAAGAATGGTTCAACACCCGCAACGACGCCTTATTCGACCGCATCTTCCTCATCCTCAAATCCTCCACCGACGACGACGTCTTGGCCCTCTCCCATCTCAGCCTCCGCCTCTCGGAGTCCTTCGTCCTCGAGGTCCTACGCTACGGCAGCAACGACCATGACGTCCTCTCCTGCCTCAAGTTCTTCGACTGGGCCGGCCGCCAGCCCGGCTTCCACCACACCCGCGCCACCTTCAACGCCATCTTCAAGATCCTCTCCAAGGCCAAGCTCATGTCCCTCATGCTCGACGTCCTCTCCACCTACAGCAAGCAGAGGTACGCCCACACCGTCCGATTTCACGACACGCTGGTCATGGGCTACGCTGTCGCCGGCAAGCCCGACATTGCCCTCCAACTGTTTGGTAAAATGCGCTTCCAGGGTTTGGACCTCGATTCCTTTGCCTACCATGTCCTTTTGAATGCTTTGGTGGAGGAGAGTTGCCTTGATGCCGTTCAGGTCATTGCCAAGCAGATTTCTTTGAGGGGTTTTGAGAACGAAATCACCCATTCCATCATGCTCAAGTGGTTGTGTAAGCAGAAGTTGCTCGATCAAGCTGAAGCCTATTTGCGCCAACTGGTGTCTGACGGCAAGCCCGTCACCGGGCATGCAGTCAGTGTCCTTGTGGACGCCCTTTGTAAGCATAAGAAGTTCCAGCAGGCTGGTGAATTAGTCGAGGAGTTTCGGGACTTGGGGGTGGCTCTCATGGAGAGTGCTTATGGTGTGTGGATACGCGATCTTGTTCAGGCTGGGAGGTTGGATGGAGCATTGGAGTTTTTTCAGAACAAGAAGTCGTTGGAAGGATATGTGCCCGATGCTTTTAGGTACAATACTTTGATATGTAGGCTTCTGAAAGAGGATCGCCTCGAGGAGGTTTGTGATTTGCTGATGGAGATGAAGGAGGATAAGATTTCCCCTGATAAGGTCACCATGAATGCTGCCTTATGCTTCTTTTGCAAAGCGGGGATGGTGGATGTCGCACTTGAGTTGTATAATTCGAGATCCGAGTTTCAGCTCACGCCTAATAGTATGGTTTACAACTATTTGATCAATGTTTTCTGTGGGGAAGGTAGCATTGACGACGCGTATCGAGTGATGAAGCATTCTGTGGAACAAGGTTATTTTACAGGAAGGAAAACGTTTTCTATTCTGGCAGACTCATTGTGCCGAGAGGGAAAGCTCGAGAAGATGAAAGAGTTAGTTACTTTTGCCCTAGAGCGGAACTTTATGCCAAGTAACTCCACATATGATAAGTTCATTACAACTCTTTGCAGAACTAAGAGGGTAGAAGATGCTTATTTGATACATGGGGAACTCAATAGAATTAACAAGGTTTCTACAAAGAGTAGATACATTAGCTTGATAAATGGTTTTAACGGGTTGAGCAAGGGGGATATTGCTGCCAGACTTCTGATTGAAATGCAAGAAAAGGGTCACTTACCGACTCGCAAGCTGTTCAAAGATGTAATTTGTTGTCTATGTGATATGGAAAATCCAGATGAGCAATTTTTTAATTTGTTGGAGATGCAGTTGTCTTGTAGGGAACCCAGCTGTGATGTATACAACTTTTTCATCTACGGAGCTGGGTATGCCAAAAGGCCCGATCTGGCTAGACAAGTATATGAGATGATGCAGCGGAGTGGAATTCAGCCCAATGTGAGATCTGATGTTATTATGTTGCAGAGTTATTTGAAGGGTGAACGGATTTCGGATGCTTTGAACTTCTTCAATGATCTGCACGAGAGAAGAAAGGTGTTGGGGAGAAGGCTATACAGCACCCTGATCGTTGGTCTATGCAAAGCCCAAAAGGCGGAAATTGCAGTTAACTTCTTGATGGAAATGAAAGAGAAGGGAGTGGTTCCAAGTGATGACTGTTACGAGTTTCTGATACAGTTGCTGTGCTGGAATAAAGAATTTGATATGGCAGTAAATCTCATAAATGACTTGGAGAAAGTTGGCCGCCATATTACGTCCTTTACTGGTAACATACTTCTGTTGCATTCTCTGAAAACTGCAGAGCTCTACAAGTCTTGGGTACAGCTGAGAGAGGAGCAGAATGAGATGTCTGGTTGTTCAATGCTTGGTCTGCTCATTGGTGCATTTTCTGGTCGTATCCAGGTGAGCCAAGATATTGGGAATTTGGAAGAAGTAATTGAAAAGTGCTTTCCGCTAGATGTGTTCACACATAATTTGTTGATTAGAAGACTAAGCCAGAGCAACATGGAGCATGCTTGTGCTTTGTTCGATAAGATGTGCCAGAAAGGTTATGAGCCTAATCGTTGGACTTATGACGCCATAGTACAAGGTTTTCTTAAACATGGGAGGACATCTGAGGCAAAGAGATGGGTAGAAGTGATGTACCGGAAAGGATTTTATCCAACAAGGCGCACAAATCTACTCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

891

Amino Acids

101.39

Weight (kDa)

8.01

Isoelectric Point (pI)

36.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	198 - 313	4.3e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	360 - 402	7.7e-09	PPR repeat family
PPR_2	PF13041	430 - 479	1.3e-06	PPR repeat family
PPR_2	PF13041	680 - 723	1e-07	PPR repeat family
PPR_2	PF13041	814 - 859	4.3e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71210
fragaria_vesca	FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690
malus_domestica	MD05G1041400.v1.1 MD10G1047800.v1.1
prunus_persica	Prupe.8G057300_v2.0.a1 Prupe.8G057300_v2.0.a1
pyrus_communis	pycom05g03410 pycom10g03380 pycom17g22250
rosa_chinensis	RchiOBHm_Chr5g0055041
rosa_laevigata	RLG00000034959
rosa_multiflora	Rmu_sc0000925.1_g000011
rosa_roxburghii	Rroxscaffold_1G00025450
rosa_rugosa	Rorug05G0290400
rosa_samantha	Rh5AG360300 Rh5BG371300 Rh5CG393600 Rh5DG385100
rosa_wichuraiana	Rw5G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	308, 392, 578
AatII	GACGTC	3 cut(s)	312, 396, 522
AbsI	CCTCGAGG	2 cut(s)	359, 1133
AccB7I	CCANNNNNTGG	1 cut(s)	652
AccBSI	CCGCTC	2 cut(s)	176, 1486
AccI	GTMKAC	1 cut(s)	1827
AccII	CGCG	3 cut(s)	456, 1002, 1352
AclI	AACGTT	1 cut(s)	1406
AclWI	GGATC	3 cut(s)	475, 1134, 1263
AcoI	YGGCCR	2 cut(s)	427, 2224
AcsI	RAATTY	3 cut(s)	1911, 2180, 2404
AcuI	CTGAAG	2 cut(s)	127, 846
AcyI	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
AfaI	GTAC	5 cut(s)	548, 1094, 2304, 2568, 2627
AfiI	CCNNNNNNNGG	9 cut(s)	343, 490, 652, 869, 870, 1138, 1189, 1485, 2077
AflIII	ACRYGT	1 cut(s)	1350
AjiI	CACGTC	1 cut(s)	217
AjnI	CCWGG	2 cut(s)	645, 2382
Alw21I	GWGCWC	1 cut(s)	2289
AlwI	GGATC	3 cut(s)	475, 1134, 1263
AlwNI	CAGNNNCTG	1 cut(s)	2164
Ama87I	CYCGRG	3 cut(s)	359, 1133, 1448
AoxI	GGCC	6 cut(s)	315, 423, 427, 492, 1865, 2224
ApeKI	GCWGC	7 cut(s)	60, 116, 119, 378, 1205, 1667, 1900
ApoI	RAATTY	3 cut(s)	1911, 2180, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	248, 280
Asp700I	GAANNNNTTC	1 cut(s)	1991
AspLEI	GCGC	4 cut(s)	458, 642, 840, 2655
AspS9I	GGNCC	6 cut(s)	220, 316, 364, 423, 655, 1866
AsuC2I	CCSGG	2 cut(s)	438, 871
AsuHPI	GGTGA	7 cut(s)	91, 763, 859, 944, 1186, 1976, 2398
AvaI	CYCGRG	3 cut(s)	359, 1133, 1448
AvaII	GGWCC	3 cut(s)	220, 364, 655
BaeGI	GKGCMC	1 cut(s)	1080
BanII	GRGCYC	1 cut(s)	2289
BauI	CACGAG	1 cut(s)	2006
BbsI	GAAGAC	1 cut(s)	2476
Bbv12I	GWGCWC	1 cut(s)	2289
BbvCI	CCTCAGC	1 cut(s)	190
BbvI	GCAGC	7 cut(s)	47, 103, 106, 390, 1192, 1654, 1912
BccI	CCATC	9 cut(s)	83, 333, 479, 788, 1022, 1150, 1228, 2095, 2604
BceAI	ACGGC	3 cut(s)	391, 704, 871
BcgI	CGANNNNNNTGC	4 cut(s)	650, 684, 2032, 2066
BciT130I	CCWGG	2 cut(s)	647, 2384
BciVI	GTATCC	2 cut(s)	990, 2390
BclI	TGATCA	1 cut(s)	1314
BcnI	CCSGG	2 cut(s)	438, 871
BfaI	CTAG	4 cut(s)	23, 1481, 1878, 2438
BfmI	CTRYAG	2 cut(s)	532, 2280
BfuI	GTATCC	2 cut(s)	990, 2390
BglI	GCCNNNNNGGC	2 cut(s)	438, 2075
BglII	AGATCT	1 cut(s)	1928
Bme1390I	CCNGG	4 cut(s)	438, 647, 871, 2384
Bme18I	GGWCC	3 cut(s)	220, 364, 655
BmeT110I	CYCGRG	3 cut(s)	359, 1133, 1448
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	6 cut(s)	220, 316, 364, 423, 655, 1866
BmiI	GGNNCC	2 cut(s)	1812, 2127
BmrFI	CCNGG	4 cut(s)	438, 647, 871, 2384
BmrI	ACTGGG	1 cut(s)	430
BmsI	GCATC	8 cut(s)	73, 282, 706, 1072, 1549, 1782, 1887, 1969
BmuI	ACTGGG	1 cut(s)	430
BpiI	GAAGAC	1 cut(s)	2476
Bpu10I	CCTNAGC	1 cut(s)	190
BpuEI	CTTGAG	3 cut(s)	392, 773, 1271
BpuMI	CCSGG	2 cut(s)	438, 871
BsaHI	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
BsaJI	CCNNGG	3 cut(s)	33, 489, 646
BsaWI	WCCGGW	1 cut(s)	2628
BsaXI	ACNNNNNCTCC	6 cut(s)	383, 413, 509, 539, 1781, 1811
Bsc4I	CCNNNNNNNGG	9 cut(s)	343, 490, 652, 869, 870, 1138, 1189, 1485, 2077
Bse118I	RCCGGY	2 cut(s)	425, 599
Bse1I	ACTGG	3 cut(s)	425, 849, 2251
Bse3DI	GCAATG	2 cut(s)	612, 729
BseBI	CCWGG	2 cut(s)	647, 2384
BseDI	CCNNGG	3 cut(s)	33, 489, 646
BseGI	GGATG	6 cut(s)	75, 282, 1033, 1239, 1246, 1984
BseLI	CCNNNNNNNGG	9 cut(s)	343, 490, 652, 869, 870, 1138, 1189, 1485, 2077
BseMI	GCAATG	2 cut(s)	612, 729
BseMII	CTCAG	5 cut(s)	28, 204, 343, 2300, 2589
BseNI	ACTGG	3 cut(s)	425, 849, 2251
BseRI	GAGGAG	5 cut(s)	55, 716, 959, 1151, 2330
BseSI	GKGCMC	1 cut(s)	1080
BseX3I	CGGCCG	1 cut(s)	427
BseXI	GCAGC	7 cut(s)	47, 103, 106, 390, 1192, 1654, 1912
BseYI	CCCAGC	3 cut(s)	1016, 1814, 1850
BsgI	GTGCAG	1 cut(s)	1988
Bsh1236I	CGCG	3 cut(s)	456, 1002, 1352
Bsh1285I	CGRYCG	2 cut(s)	271, 430
BshFI	GGCC	6 cut(s)	317, 425, 429, 494, 1867, 2226
BsiEI	CGRYCG	2 cut(s)	271, 430
BsiHKAI	GWGCWC	1 cut(s)	2289
BsiHKCI	CYCGRG	3 cut(s)	359, 1133, 1448
BsiSI	CCGG	6 cut(s)	206, 426, 438, 600, 870, 2629
BslFI	GGGAC	3 cut(s)	227, 490, 968
BslI	CCNNNNNNNGG	9 cut(s)	343, 490, 652, 869, 870, 1138, 1189, 1485, 2077
BsmFI	GGGAC	3 cut(s)	227, 490, 968
BsmI	GAATGC	4 cut(s)	694, 1207, 1369, 2266
BsnI	GGCC	6 cut(s)	317, 425, 429, 494, 1867, 2226
BsoBI	CYCGRG	3 cut(s)	359, 1133, 1448
Bsp1286I	GDGCHC	2 cut(s)	1080, 2289
BspANI	GGCC	6 cut(s)	317, 425, 429, 494, 1867, 2226
BspCNI	CTCAG	5 cut(s)	27, 203, 342, 2301, 2590
BspFNI	CGCG	3 cut(s)	456, 1002, 1352
BspLI	GGNNCC	2 cut(s)	1812, 2127
BspMAI	CTGCAG	1 cut(s)	2284
BspPI	GGATC	3 cut(s)	475, 1134, 1263
BsrBI	CCGCTC	2 cut(s)	176, 1486
BsrDI	GCAATG	2 cut(s)	612, 729
BsrFI	RCCGGY	2 cut(s)	425, 599
BsrI	ACTGG	3 cut(s)	425, 849, 2251
BssAI	RCCGGY	2 cut(s)	425, 599
BssECI	CCNNGG	3 cut(s)	33, 489, 646
BssNAI	GTATAC	1 cut(s)	1828
BssNI	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
BssSI	CACGAG	1 cut(s)	2006
BssT1I	CCWWGG	2 cut(s)	33, 489
Bst1107I	GTATAC	1 cut(s)	1828
Bst2BI	CACGAG	1 cut(s)	2006
Bst2UI	CCWGG	2 cut(s)	647, 2384
Bst4CI	ACNGT	4 cut(s)	559, 627, 2141, 2160
BstACI	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
BstAPI	GCANNNNNTGC	1 cut(s)	811
BstDEI	CTNAG	7 cut(s)	14, 190, 329, 1545, 2309, 2474, 2598
BstEII	GGTNACC	1 cut(s)	1192
BstENI	CCTNNNNNAGG	2 cut(s)	488, 1136
BstF5I	GGATG	6 cut(s)	75, 282, 1033, 1239, 1246, 1984
BstFNI	CGCG	3 cut(s)	456, 1002, 1352
BstHHI	GCGC	4 cut(s)	458, 642, 840, 2655
BstMCI	CGRYCG	2 cut(s)	271, 430
BstNI	CCWGG	2 cut(s)	647, 2384
BstNSI	RCATGY	2 cut(s)	878, 2498
BstPI	GGTNACC	1 cut(s)	1192
BstSCI	CCNGG	4 cut(s)	436, 645, 869, 2382
BstSFI	CTRYAG	2 cut(s)	532, 2280
BstSLI	GKGCMC	1 cut(s)	1080
BstUI	CGCG	3 cut(s)	456, 1002, 1352
BstV1I	GCAGC	7 cut(s)	47, 103, 106, 390, 1192, 1654, 1912
BstV2I	GAAGAC	1 cut(s)	2476
BstX2I	RGATCY	3 cut(s)	480, 1268, 1928
BstXI	CCANNNNNNTGG	3 cut(s)	629, 929, 2399
BstYI	RGATCY	3 cut(s)	480, 1268, 1928
BstZ17I	GTATAC	1 cut(s)	1828
BstZI	CGGCCG	1 cut(s)	427
BsuI	GTATCC	2 cut(s)	990, 2390
BsuRI	GGCC	6 cut(s)	317, 425, 429, 494, 1867, 2226
BtrI	CACGTC	1 cut(s)	217
BtsCI	GGATG	6 cut(s)	75, 282, 1033, 1239, 1246, 1984
BtsIMutI	CAGTG	1 cut(s)	889
CaiI	CAGNNNCTG	1 cut(s)	2164
CfoI	GCGC	4 cut(s)	458, 642, 840, 2655
Cfr10I	RCCGGY	2 cut(s)	425, 599
Cfr13I	GGNCC	6 cut(s)	220, 316, 364, 423, 655, 1866
CseI	GACGC	4 cut(s)	266, 905, 1358, 2567
Csp6I	GTAC	5 cut(s)	547, 1093, 2303, 2567, 2626
CspCI	CAANNNNNGTGG	2 cut(s)	198, 233
CviQI	GTAC	5 cut(s)	547, 1093, 2303, 2567, 2626
DdeI	CTNAG	7 cut(s)	14, 190, 329, 1545, 2309, 2474, 2598
DrdI	GACNNNNNNGTC	3 cut(s)	308, 392, 578
DseDI	GACNNNNNNGTC	3 cut(s)	308, 392, 578
EaeI	YGGCCR	2 cut(s)	427, 2224
EagI	CGGCCG	1 cut(s)	427
EciI	GGCGGA	3 cut(s)	58, 326, 2093
Ecl136II	GAGCTC	1 cut(s)	2287
EclXI	CGGCCG	1 cut(s)	427
Eco130I	CCWWGG	2 cut(s)	33, 489
Eco24I	GRGCYC	1 cut(s)	2289
Eco47I	GGWCC	3 cut(s)	220, 364, 655
Eco52I	CGGCCG	1 cut(s)	427
Eco53kI	GAGCTC	1 cut(s)	2287
Eco57I	CTGAAG	2 cut(s)	127, 846
Eco88I	CYCGRG	3 cut(s)	359, 1133, 1448
Eco91I	GGTNACC	1 cut(s)	1192
EcoICRI	GAGCTC	1 cut(s)	2287
EcoNI	CCTNNNNNAGG	2 cut(s)	488, 1136
EcoO109I	RGGNCCY	1 cut(s)	364
EcoO65I	GGTNACC	1 cut(s)	1192
EcoRI	GAATTC	1 cut(s)	1911
EcoRII	CCWGG	2 cut(s)	645, 2382
EcoT14I	CCWWGG	2 cut(s)	33, 489
EcoT38I	GRGCYC	1 cut(s)	2289
ErhI	CCWWGG	2 cut(s)	33, 489
FalI	AAGNNNNNCTT	4 cut(s)	1688, 1720, 2562, 2594
FaqI	GGGAC	3 cut(s)	227, 490, 968
FauI	CCCGC	4 cut(s)	181, 257, 461, 1222
FauNDI	CATATG	1 cut(s)	1513
FbaI	TGATCA	1 cut(s)	1314
FblI	GTMKAC	1 cut(s)	1827
FokI	GGATG	6 cut(s)	62, 269, 1040, 1246, 1253, 1991
FriOI	GRGCYC	1 cut(s)	2289
FseI	GGCCGGCC	1 cut(s)	429
FspBI	CTAG	4 cut(s)	23, 1481, 1878, 2438
GlaI	GCGC	4 cut(s)	457, 641, 839, 2654
GsaI	CCCAGC	3 cut(s)	1020, 1818, 1854
HaeIII	GGCC	6 cut(s)	317, 425, 429, 494, 1867, 2226
HapII	CCGG	6 cut(s)	206, 426, 438, 600, 870, 2629
HgaI	GACGC	4 cut(s)	266, 905, 1358, 2567
HhaI	GCGC	4 cut(s)	458, 642, 840, 2655
Hin1I	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
Hin6I	GCGC	4 cut(s)	456, 640, 838, 2653
HinP1I	GCGC	4 cut(s)	456, 640, 838, 2653
HincII	GTYRAC	1 cut(s)	2092
HindII	GTYRAC	1 cut(s)	2092
HinfI	GANTC	5 cut(s)	202, 347, 662, 1424, 1711
HpaI	GTTAAC	1 cut(s)	2092
HpaII	CCGG	6 cut(s)	206, 426, 438, 600, 870, 2629
HphI	GGTGA	7 cut(s)	91, 763, 859, 944, 1186, 1976, 2398
Hpy166II	GTNNAC	6 cut(s)	895, 1303, 1828, 1967, 2092, 2448
Hpy188III	TCNNGA	7 cut(s)	478, 569, 953, 1266, 1450, 1763, 2098
Hpy8I	GTNNAC	6 cut(s)	895, 1303, 1828, 1967, 2092, 2448
Hpy99I	CGWCG	6 cut(s)	260, 305, 308, 311, 521, 1352
HpyCH4III	ACNGT	4 cut(s)	559, 627, 2141, 2160
HpyCH4IV	ACGT	6 cut(s)	216, 309, 393, 519, 1406, 2237
HpyF3I	CTNAG	7 cut(s)	14, 190, 329, 1545, 2309, 2474, 2598
HpySE526I	ACGT	6 cut(s)	216, 309, 393, 519, 1406, 2237
Hsp92I	GRCGYC	6 cut(s)	258, 309, 393, 519, 897, 2559
HspAI	GCGC	4 cut(s)	456, 640, 838, 2653
KroI	GCCGGC	2 cut(s)	425, 599
KroNI	GCCGGC	2 cut(s)	427, 601
Ksp22I	TGATCA	1 cut(s)	1314
KspAI	GTTAAC	1 cut(s)	2092
LmnI	GCTCC	7 cut(s)	56, 68, 181, 1031, 1847, 2317, 2491
Lsp1109I	GCAGC	7 cut(s)	47, 103, 106, 390, 1192, 1654, 1912
LweI	GCATC	8 cut(s)	73, 282, 706, 1072, 1549, 1782, 1887, 1969
MaeI	CTAG	4 cut(s)	23, 1481, 1878, 2438
MaeII	ACGT	6 cut(s)	216, 309, 393, 519, 1406, 2237
MaeIII	GTNAC	7 cut(s)	865, 1192, 1468, 1502, 1700, 2141, 2249
MbiI	CCGCTC	2 cut(s)	176, 1486
MflI	RGATCY	3 cut(s)	480, 1268, 1928
MhlI	GDGCHC	2 cut(s)	1080, 2289
MluI	ACGCGT	1 cut(s)	1350
MlyI	GAGTC	4 cut(s)	196, 356, 1418, 1705
MmeI	TCCRAC	6 cut(s)	646, 1005, 1044, 1767, 2528, 2669
MroNI	GCCGGC	2 cut(s)	425, 599
MroXI	GAANNNNTTC	1 cut(s)	1991
MseI	TTAA	6 cut(s)	1593, 1641, 1779, 2091, 2580, 2671
MslI	CAYNNNNRTG	1 cut(s)	2499
MspA1I	CMGCKG	4 cut(s)	116, 1818, 1903, 2308
MspI	CCGG	6 cut(s)	206, 426, 438, 600, 870, 2629
MspR9I	CCNGG	4 cut(s)	438, 647, 871, 2384
Mva1269I	GAATGC	4 cut(s)	694, 1207, 1369, 2266
MvaI	CCWGG	2 cut(s)	647, 2384
MvnI	CGCG	3 cut(s)	456, 1002, 1352
NaeI	GCCGGC	2 cut(s)	427, 601
NciI	CCSGG	2 cut(s)	438, 871
NdeI	CATATG	1 cut(s)	1513
NgoMIV	GCCGGC	2 cut(s)	425, 599
NlaIV	GGNNCC	2 cut(s)	1812, 2127
NmeAIII	GCCGAG	1 cut(s)	1460
NmuCI	GTSAC	3 cut(s)	865, 1192, 1700
NspI	RCATGY	2 cut(s)	878, 2498
PaeI	GCATGC	2 cut(s)	878, 2498
PaeR7I	CTCGAG	3 cut(s)	359, 1133, 1448
PcsI	WCGNNNNNNNCGW	5 cut(s)	264, 381, 390, 555, 861
PctI	GAATGC	4 cut(s)	694, 1207, 1369, 2266
PdiI	GCCGGC	2 cut(s)	427, 601
PdmI	GAANNNNTTC	1 cut(s)	1991
PfeI	GAWTC	1 cut(s)	662
PflFI	GACNNNGTC	2 cut(s)	218, 308
PflMI	CCANNNNNTGG	1 cut(s)	652
PleI	GAGTC	4 cut(s)	196, 355, 1418, 1705
PpsI	GAGTC	4 cut(s)	196, 355, 1418, 1705
PpuMI	RGGWCCY	1 cut(s)	364
Psp124BI	GAGCTC	1 cut(s)	2289
Psp1406I	AACGTT	1 cut(s)	1406
Psp5II	RGGWCCY	1 cut(s)	364
Psp6I	CCWGG	2 cut(s)	645, 2382
PspEI	GGTNACC	1 cut(s)	1192
PspFI	CCCAGC	3 cut(s)	1016, 1814, 1850
PspGI	CCWGG	2 cut(s)	645, 2382
PspN4I	GGNNCC	2 cut(s)	1812, 2127
PspPI	GGNCC	6 cut(s)	220, 316, 364, 423, 655, 1866
PspPPI	RGGWCCY	1 cut(s)	364
PspXI	VCTCGAGB	2 cut(s)	359, 1133
PstI	CTGCAG	1 cut(s)	2284
PstNI	CAGNNNCTG	1 cut(s)	2164
PsuI	RGATCY	3 cut(s)	480, 1268, 1928
PsyI	GACNNNGTC	2 cut(s)	218, 308
PvuII	CAGCTG	2 cut(s)	1818, 2308
RigI	GGCCGGCC	1 cut(s)	429
RsaI	GTAC	5 cut(s)	548, 1094, 2304, 2568, 2627
RsaNI	GTAC	5 cut(s)	547, 1093, 2303, 2567, 2626
RseI	CAYNNNNRTG	1 cut(s)	2499
SacI	GAGCTC	1 cut(s)	2289
SaqAI	TTAA	6 cut(s)	1593, 1641, 1779, 2091, 2580, 2671
Sau96I	GGNCC	6 cut(s)	220, 316, 364, 423, 655, 1866
SchI	GAGTC	4 cut(s)	196, 356, 1418, 1705
ScrFI	CCNGG	4 cut(s)	438, 647, 871, 2384
SduI	GDGCHC	2 cut(s)	1080, 2289
SfaNI	GCATC	8 cut(s)	73, 282, 706, 1072, 1549, 1782, 1887, 1969
SfcI	CTRYAG	2 cut(s)	532, 2280
Sfr274I	CTCGAG	3 cut(s)	359, 1133, 1448
SinI	GGWCC	3 cut(s)	220, 364, 655
SlaI	CTCGAG	3 cut(s)	359, 1133, 1448
SmiMI	CAYNNNNRTG	1 cut(s)	2499
SmlI	CTYRAG	6 cut(s)	359, 407, 788, 1133, 1250, 1448
SmoI	CTYRAG	6 cut(s)	359, 407, 788, 1133, 1250, 1448
SphI	GCATGC	2 cut(s)	878, 2498
SspMI	CTAG	4 cut(s)	23, 1481, 1878, 2438
SstI	GAGCTC	1 cut(s)	2289
StyD4I	CCNGG	4 cut(s)	436, 645, 869, 2382
StyI	CCWWGG	2 cut(s)	33, 489
TaaI	ACNGT	4 cut(s)	559, 627, 2141, 2160
TaiI	ACGT	6 cut(s)	219, 312, 396, 522, 1409, 2240
TatI	WGTACW	1 cut(s)	2566
TauI	GCSGC	5 cut(s)	74, 113, 116, 432, 2229
TfiI	GAWTC	1 cut(s)	662
Tru1I	TTAA	6 cut(s)	1593, 1641, 1779, 2091, 2580, 2671
Tru9I	TTAA	6 cut(s)	1593, 1641, 1779, 2091, 2580, 2671
TscAI	CASTG	1 cut(s)	889
TseFI	GTSAC	3 cut(s)	865, 1192, 1700
TseI	GCWGC	7 cut(s)	60, 116, 119, 378, 1205, 1667, 1900
Tsp45I	GTSAC	3 cut(s)	865, 1192, 1700
TspDTI	ATGAA	7 cut(s)	1178, 1214, 1379, 1472, 1513, 1828, 2123
TspGWI	ACGGA	2 cut(s)	1860, 1984
TspRI	CASTG	1 cut(s)	889
Tth111I	GACNNNGTC	2 cut(s)	218, 308
Van91I	CCANNNNNTGG	1 cut(s)	652
VpaK11BI	GGWCC	3 cut(s)	220, 364, 655
XagI	CCTNNNNNAGG	2 cut(s)	488, 1136
XapI	RAATTY	3 cut(s)	1911, 2180, 2404
XceI	RCATGY	2 cut(s)	878, 2498
XhoI	CTCGAG	3 cut(s)	359, 1133, 1448
XmiI	GTMKAC	1 cut(s)	1827
XmnI	GAANNNNTTC	1 cut(s)	1991
XspI	CTAG	4 cut(s)	23, 1481, 1878, 2438
ZraI	GACGTC	3 cut(s)	310, 394, 520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.