Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007971

Physical Location & Seq

Genomic Coordinates Forward (+)

6379409 .. 6382183

Gene Structure 2775 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1047800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2682 bp

ATGCTTATTTCACTCAGAAATCTAGCCAAATCCAAGGCCAATACCAACTGCTCCCTTTGCTCCTCCTTCGCCGCCATCCTCGATGCCAATTCTCTTCTCTCCACCGCCGCCGCCGCCGCTGCTACCACCAGCAGCAGCGTCAGCGGTGGTTGTTATTGCTTTTGTTGTTCCTTGAGCCCCAACGCTTCCCCTGCCGCCGCTGCTTTCTCAACCATGGCCCGCATGACTGCGGCGAGGGACGTGGTCCTCTCTTTCAAAGAATGGTTCCAGACCCGCAACGACGCCTTATTGGACCGCATCTTCCTCATCCTCAAATCCTCTACCGACGGCGACCCCGCCGCCACCACCGACCGCCGCCTCAACGGCGACAGCGACATCCCCTTGCGCTATAATATCCCTTACTCCTCCGCCGACTTGGCCCTCTCCCGTCTCAGTCTCCGCCTCACGGAGGCCTTCGTCCTCGAGGTCCTCCGCTACGGCAGCAACGACCATGATGTCCTCTCCTGCCTCAAGTTCTTCGATTGGGCAGGCCGCCAGCCCAGCTTCCACCACACCCGCGCCACCTTTAACGCCATCTTCAAGATCCTTTCCAAGGCCAAGCTCATGTCTCTCATGCTCGACTTCCTCTCCACCTACCGCAAGCAGAGGTACGCCCACACCGTCCGATTTCACGACACTCTGGTCATGGGCTACGCCGTCGCCGGCAAGCCCTACATTGCCCTCCAACTGTTTGGTAAAATGCGCTTCCAGGGTCTCGACCTCGATTCCTTTGGCTACCATGTCCTTCTGAATGCTCTAGTGGAGGAGAGTTGCTTTGATGCTGTTCAGGTCATTGCCAAGCAGATTTCTTTGAGGGGTTTCGAGAACGAAATTACCCATTCCATCATGCTCAAGTGGTTCTGTAAGCAGAACTTGCTCGATCAAGCTGAAGCCTATTTGCGTCAATTGGTGTCTGACGGCAAGCCGGTCACTGGCCATGCCGTCAGTGTCCTTGTGGACGCCCTTTGTAAGCAGAAGAAGTTCCAGCATGCTGGCCAATTAGTCGAGGAGTTTCAGGACCTTGGGGCTGGGAGGTTGGATGGAGCATTGGAGTTTTTGCAGAACAAGAAGTCGTTGGAAGGGCTTTTGAAGGAGGATCGCCTCGAGGAGGTTTGTGATTTGTTGATGGAGATGAAGGAGGGTAAGATTTCCCCTGATAAGATCACCATGAATGCTGCCTTGTGCTTCTTCTGCAAAGCTGGGATGGTGGATGTCGCACTTGAGTTGTATAATTCGAGATCTGAGTTTGGGCTCACTCCTAATAGTATGGTTTACAACTATTTGATCAATATTTTCTGTGGGGATGGTAGCATTGACGAAGCCTATCGAGTGTTGAAGCATTCTATGGAACAAGGGTATTTTACAGGTAGGAAAACATTTTCTATTCTGGCTGACTCATTGTGTCGAGAGGGCAAGCTTGAGAAGATGAAAGAGTTGGTTATTTTCGCCTTAGAGCGAAACTTTATGCCAAGTGACTATACATTTGATAAGTTCATTACAACTCTTTGCAGTACTGAGAGGGTAGAAGACGGTTATTTGGTACATGCGGAACTCAACAGAATTAATAAGGTTTCTAAAAAGAAGACATACTTTAGCTTGATAAATGGTTTTAACAGGTCGAGCAAGGGGGATATTGCTGCCAGGCTTCTGATTGAAATGCAAGAAAAGGGTCACTTACCAATTCGTAAACTGTTCAAAGATGTAATTTGCTGTCTCTGTGATATGGAAAATCCAGATAAGCAATTTTTTACTTTGTTGGAGATGCAGTTGTCTTGTAGGGAACCCAGCCGTGATATATACAACTTTTTCATCTACGGAGCTGGGCATGCCAAAAGGCCCGATCTGGCTAGACAAGTATTTGAGATGATGCAGCGGAGTGGGATTGATCCCAATGTGAGATCGGATGTTCTTATGTTGCAGAGTTATTTGAAGAGTGAACGAATTTCGGATGCATTGAACTTCTTCAATGATCTGCACCAGAGAAGAAAGGTGATGGGGAGAAAGCTATACAGTGCCCTGATCATTGGTCTATGCAAAGCCAAAAAGGTGGAAATTGCAGTTAACTTCTTGATGGAAATGAAAGAGAAGGGAGTGGTTCCAAGCGATGACTGTTACGAGTTTCTGATACAGTTGCTGTGCCAGAATAAAGAGTTTGATATGGCAGTAAATCTCATAAATGACTTGGAGAAAGTTGGCCGCCATATTACATCCTTTACCGGTAACATACTTCTGTTGCAATCTCTGAAAACTGCAGAGCTCTACAAGTCTTGGGTACGGCTGAGAGAGGAGCGGAATGAGATGTCTGATCGTTCAATGCTTGGTCTGCTCATTGGTGCGTTTTCTGGTCAGATTAAGGTGAGCCGAGATATTGGGAATCTGGAAGAAGCAATTGAAATGTGCTTTCCGCTAGACGTGTTCACACACAATTTGTTGATTAGAAGACTAAGCCAAAGCAACATGGAGCACGCTTGTGCTTTGTTCGAAAAGATGTGCCAGAAAGGTTATGAGCCTAATCGATGGACTTACGACGCCTTAGTAGAAGGTTTTCTTAGACATGGGAGGACATCTGAGGCAAAGAAATGGGTAGAAGTGATGTACCGGAAAGGGTTTTATCCAACAGGGCGCACAAATCTACTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

894

Amino Acids

101.36

Weight (kDa)

8.38

Isoelectric Point (pI)

36.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	398 - 446	1.4e-08	PPR repeat family
PPR_1	PF12854	430 - 459	4.4e-06	PPR repeat
PPR_2	PF13041	433 - 482	4.4e-06	PPR repeat family
PPR_2	PF13041	683 - 726	3.5e-08	PPR repeat family
PPR_2	PF13041	817 - 862	3.8e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71210
fragaria_vesca	FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690
malus_domestica	MD05G1041400.v1.1 MD10G1047800.v1.1
prunus_persica	Prupe.8G057300_v2.0.a1 Prupe.8G057300_v2.0.a1
pyrus_communis	pycom05g03410 pycom10g03380 pycom17g22250
rosa_chinensis	RchiOBHm_Chr5g0055041
rosa_laevigata	RLG00000034959
rosa_multiflora	Rmu_sc0000925.1_g000011
rosa_roxburghii	Rroxscaffold_1G00025450
rosa_rugosa	Rorug05G0290400
rosa_samantha	Rh5AG360300 Rh5BG371300 Rh5CG393600 Rh5DG385100
rosa_wichuraiana	Rw5G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	494, 680
AbsI	CCTCGAGG	2 cut(s)	461, 1142
AccBSI	CCGCTC	1 cut(s)	2329
AccII	CGCG	1 cut(s)	558
AclWI	GGATC	3 cut(s)	577, 1143, 1919
AcoI	YGGCCR	3 cut(s)	973, 1033, 2233
AcsI	RAATTY	1 cut(s)	1980
AcuI	CTGAAG	1 cut(s)	948
AcyI	GRCGYC	3 cut(s)	282, 999, 2568
AfaI	GTAC	5 cut(s)	650, 1552, 1581, 2313, 2636
AfiI	CCNNNNNNNGG	5 cut(s)	445, 448, 592, 971, 1147
AflIII	ACRYGT	1 cut(s)	2451
AgeI	ACCGGT	1 cut(s)	2255
AjiI	CACGTC	2 cut(s)	241, 2452
AjnI	CCWGG	2 cut(s)	747, 1679
AleI	CACNNNNGTG	1 cut(s)	2508
AluBI	AGCT	9 cut(s)	543, 601, 926, 1238, 1456, 1635, 1859, 2044, 2296
AluI	AGCT	9 cut(s)	543, 601, 926, 1238, 1456, 1635, 1859, 2044, 2296
Alw21I	GWGCWC	2 cut(s)	2298, 2505
Alw26I	GTCTC	5 cut(s)	434, 440, 612, 758, 1757
AlwI	GGATC	3 cut(s)	577, 1143, 1919
AlwNI	CAGNNNCTG	1 cut(s)	2173
Ama87I	CYCGRG	2 cut(s)	461, 1142
ApeKI	GCWGC	8 cut(s)	119, 132, 135, 200, 480, 1214, 1676, 1909
ApoI	RAATTY	1 cut(s)	1980
ArsI	GACNNNNNNTTYG	2 cut(s)	1505, 1537
AseI	ATTAAT	1 cut(s)	1602
AsiGI	ACCGGT	1 cut(s)	2255
Asp700I	GAANNNNTTC	3 cut(s)	1019, 2000, 2583
AspLEI	GCGC	4 cut(s)	387, 560, 744, 2664
AspS9I	GGNCC	7 cut(s)	217, 244, 292, 418, 466, 1057, 1875
AsuHPI	GGTGA	3 cut(s)	1195, 2041, 2407
AsuII	TTCGAA	1 cut(s)	2520
AvaI	CYCGRG	2 cut(s)	461, 1142
AvaII	GGWCC	4 cut(s)	244, 292, 466, 1057
BaeGI	GKGCMC	1 cut(s)	2056
BalI	TGGCCA	2 cut(s)	975, 1035
BanII	GRGCYC	3 cut(s)	179, 1293, 2298
BbsI	GAAGAC	3 cut(s)	1572, 1628, 2485
Bbv12I	GWGCWC	2 cut(s)	2298, 2505
BbvI	GCAGC	8 cut(s)	106, 144, 147, 187, 492, 1201, 1663, 1921
BceAI	ACGGC	8 cut(s)	343, 379, 493, 680, 965, 973, 1812, 2330
BciT130I	CCWGG	2 cut(s)	749, 1681
BclI	TGATCA	2 cut(s)	1323, 2058
BcoDI	GTCTC	5 cut(s)	434, 440, 612, 758, 1757
BfaI	CTAG	4 cut(s)	23, 797, 1887, 2447
BfmI	CTRYAG	1 cut(s)	2289
BglI	GCCNNNNNGGC	2 cut(s)	363, 416
BglII	AGATCT	1 cut(s)	1277
BmcAI	AGTACT	1 cut(s)	1552
Bme1390I	CCNGG	2 cut(s)	749, 1681
Bme18I	GGWCC	4 cut(s)	244, 292, 466, 1057
BmeT110I	CYCGRG	2 cut(s)	461, 1142
BmgBI	CACGTC	2 cut(s)	241, 2452
BmgT120I	GGNCC	7 cut(s)	217, 244, 292, 418, 466, 1057, 1875
BmiI	GGNNCC	3 cut(s)	266, 1821, 2136
BmrFI	CCNGG	2 cut(s)	749, 1681
BmsI	GCATC	6 cut(s)	73, 306, 808, 1791, 1896, 1978
BpiI	GAAGAC	3 cut(s)	1572, 1628, 2485
Bpu14I	TTCGAA	1 cut(s)	2520
BpuEI	CTTGAG	5 cut(s)	193, 494, 875, 1280, 1478
Bsa29I	ATCGAT	1 cut(s)	2554
BsaHI	GRCGYC	3 cut(s)	282, 999, 2568
BsaI	GGTCTC	1 cut(s)	758
BsaJI	CCNNGG	5 cut(s)	33, 213, 591, 748, 1060
BsaWI	WCCGGW	2 cut(s)	2255, 2637
BsaXI	ACNNNNNCTCC	4 cut(s)	611, 641, 1790, 1820
Bsc4I	CCNNNNNNNGG	5 cut(s)	445, 448, 592, 971, 1147
Bse118I	RCCGGY	3 cut(s)	701, 964, 2255
Bse1I	ACTGG	1 cut(s)	976
Bse3DI	GCAATG	2 cut(s)	714, 831
BseBI	CCWGG	2 cut(s)	749, 1681
BseCI	ATCGAT	1 cut(s)	2554
BseDI	CCNNGG	5 cut(s)	33, 213, 591, 748, 1060
BseLI	CCNNNNNNNGG	5 cut(s)	445, 448, 592, 971, 1147
BseMI	GCAATG	2 cut(s)	714, 831
BseMII	CTCAG	6 cut(s)	28, 445, 1272, 1545, 2309, 2598
BseNI	ACTGG	1 cut(s)	976
BseRI	GAGGAG	6 cut(s)	52, 394, 818, 1061, 1160, 2339
BseSI	GKGCMC	1 cut(s)	2056
BseXI	GCAGC	8 cut(s)	106, 144, 147, 187, 492, 1201, 1663, 1921
BseYI	CCCAGC	5 cut(s)	539, 1067, 1238, 1823, 1859
BsgI	GTGCAG	1 cut(s)	1997
Bsh1236I	CGCG	1 cut(s)	558
Bsh1285I	CGRYCG	1 cut(s)	352
BshTI	ACCGGT	1 cut(s)	2255
BshVI	ATCGAT	1 cut(s)	2554
BsiEI	CGRYCG	1 cut(s)	352
BsiHKAI	GWGCWC	2 cut(s)	2298, 2505
BsiHKCI	CYCGRG	2 cut(s)	461, 1142
BsiSI	CCGG	4 cut(s)	702, 965, 2256, 2638
BslFI	GGGAC	1 cut(s)	251
BslI	CCNNNNNNNGG	5 cut(s)	445, 448, 592, 971, 1147
BsmAI	GTCTC	5 cut(s)	434, 440, 612, 758, 1757
BsmBI	CGTCTC	1 cut(s)	434
BsmFI	GGGAC	1 cut(s)	251
BsmI	GAATGC	3 cut(s)	796, 1216, 1378
Bso31I	GGTCTC	1 cut(s)	758
BsoBI	CYCGRG	2 cut(s)	461, 1142
Bsp119I	TTCGAA	1 cut(s)	2520
Bsp1286I	GDGCHC	5 cut(s)	179, 1293, 2056, 2298, 2505
Bsp19I	CCATGG	1 cut(s)	213
BspCNI	CTCAG	6 cut(s)	27, 444, 1273, 1546, 2310, 2599
BspDI	ATCGAT	1 cut(s)	2554
BspFNI	CGCG	1 cut(s)	558
BspLI	GGNNCC	3 cut(s)	266, 1821, 2136
BspMAI	CTGCAG	1 cut(s)	2293
BspPI	GGATC	3 cut(s)	577, 1143, 1919
BspT104I	TTCGAA	1 cut(s)	2520
BspTNI	GGTCTC	1 cut(s)	758
BsrBI	CCGCTC	1 cut(s)	2329
BsrDI	GCAATG	2 cut(s)	714, 831
BsrFI	RCCGGY	3 cut(s)	701, 964, 2255
BsrI	ACTGG	1 cut(s)	976
BssAI	RCCGGY	3 cut(s)	701, 964, 2255
BssECI	CCNNGG	5 cut(s)	33, 213, 591, 748, 1060
BssNI	GRCGYC	3 cut(s)	282, 999, 2568
BssT1I	CCWWGG	4 cut(s)	33, 213, 591, 1060
Bst2UI	CCWGG	2 cut(s)	749, 1681
Bst4CI	ACNGT	7 cut(s)	661, 729, 1571, 1731, 2051, 2150, 2169
Bst6I	CTCTTC	2 cut(s)	99, 1964
BstACI	GRCGYC	3 cut(s)	282, 999, 2568
BstAPI	GCANNNNNTGC	1 cut(s)	913
BstBI	TTCGAA	1 cut(s)	2520
BstDSI	CCRYGG	1 cut(s)	213
BstENI	CCTNNNNNAGG	3 cut(s)	446, 590, 1145
BstFNI	CGCG	1 cut(s)	558
BstHHI	GCGC	4 cut(s)	387, 560, 744, 2664
BstMAI	GTCTC	5 cut(s)	434, 440, 612, 758, 1757
BstMCI	CGRYCG	1 cut(s)	352
BstNI	CCWGG	2 cut(s)	749, 1681
BstNSI	RCATGY	3 cut(s)	1031, 1586, 1868
BstSCI	CCNGG	2 cut(s)	747, 1679
BstSFI	CTRYAG	1 cut(s)	2289
BstSLI	GKGCMC	1 cut(s)	2056
BstUI	CGCG	1 cut(s)	558
BstV1I	GCAGC	8 cut(s)	106, 144, 147, 187, 492, 1201, 1663, 1921
BstV2I	GAAGAC	3 cut(s)	1572, 1628, 2485
BstX2I	RGATCY	2 cut(s)	582, 1277
BstXI	CCANNNNNNTGG	3 cut(s)	731, 1031, 2086
BstYI	RGATCY	2 cut(s)	582, 1277
Bsu15I	ATCGAT	1 cut(s)	2554
BsuTUI	ATCGAT	1 cut(s)	2554
BtgI	CCRYGG	1 cut(s)	213
BtgZI	GCGATG	1 cut(s)	2157
BtrI	CACGTC	2 cut(s)	241, 2452
BtsIMutI	CAGTG	3 cut(s)	969, 991, 2056
CaiI	CAGNNNCTG	1 cut(s)	2173
CfoI	GCGC	4 cut(s)	387, 560, 744, 2664
Cfr10I	RCCGGY	3 cut(s)	701, 964, 2255
Cfr13I	GGNCC	7 cut(s)	217, 244, 292, 418, 466, 1057, 1875
ClaI	ATCGAT	1 cut(s)	2554
CseI	GACGC	5 cut(s)	127, 290, 929, 1007, 2576
Csp6I	GTAC	5 cut(s)	649, 1551, 1580, 2312, 2635
CspAI	ACCGGT	1 cut(s)	2255
CviQI	GTAC	5 cut(s)	649, 1551, 1580, 2312, 2635
DrdI	GACNNNNNNGTC	2 cut(s)	494, 680
DseDI	GACNNNNNNGTC	2 cut(s)	494, 680
EaeI	YGGCCR	3 cut(s)	973, 1033, 2233
Eam1104I	CTCTTC	2 cut(s)	99, 1964
EarI	CTCTTC	2 cut(s)	99, 1964
EciI	GGCGGA	2 cut(s)	397, 428
Ecl136II	GAGCTC	1 cut(s)	2296
Eco130I	CCWWGG	4 cut(s)	33, 213, 591, 1060
Eco147I	AGGCCT	1 cut(s)	452
Eco24I	GRGCYC	3 cut(s)	179, 1293, 2298
Eco31I	GGTCTC	1 cut(s)	758
Eco47I	GGWCC	4 cut(s)	244, 292, 466, 1057
Eco53kI	GAGCTC	1 cut(s)	2296
Eco57I	CTGAAG	1 cut(s)	948
Eco88I	CYCGRG	2 cut(s)	461, 1142
EcoICRI	GAGCTC	1 cut(s)	2296
EcoNI	CCTNNNNNAGG	3 cut(s)	446, 590, 1145
EcoO109I	RGGNCCY	2 cut(s)	466, 1057
EcoRII	CCWGG	2 cut(s)	747, 1679
EcoT14I	CCWWGG	4 cut(s)	33, 213, 591, 1060
EcoT22I	ATGCAT	1 cut(s)	1993
EcoT38I	GRGCYC	3 cut(s)	179, 1293, 2298
ErhI	CCWWGG	4 cut(s)	33, 213, 591, 1060
Esp3I	CGTCTC	1 cut(s)	434
FalI	AAGNNNNNCTT	8 cut(s)	896, 928, 1613, 1645, 1697, 1729, 2571, 2603
FaqI	GGGAC	1 cut(s)	251
FauI	CCCGC	4 cut(s)	227, 281, 343, 563
FbaI	TGATCA	2 cut(s)	1323, 2058
FriOI	GRGCYC	3 cut(s)	179, 1293, 2298
FspBI	CTAG	4 cut(s)	23, 797, 1887, 2447
GlaI	GCGC	4 cut(s)	386, 559, 743, 2663
GsaI	CCCAGC	5 cut(s)	543, 1071, 1242, 1827, 1863
HapII	CCGG	4 cut(s)	702, 965, 2256, 2638
HgaI	GACGC	5 cut(s)	127, 290, 929, 1007, 2576
HhaI	GCGC	4 cut(s)	387, 560, 744, 2664
Hin1I	GRCGYC	3 cut(s)	282, 999, 2568
Hin6I	GCGC	4 cut(s)	385, 558, 742, 2662
HinP1I	GCGC	4 cut(s)	385, 558, 742, 2662
HincII	GTYRAC	1 cut(s)	2101
HindII	GTYRAC	1 cut(s)	2101
HindIII	AAGCTT	1 cut(s)	1454
HinfI	GANTC	3 cut(s)	764, 1433, 2413
HpaI	GTTAAC	1 cut(s)	2101
HpaII	CCGG	4 cut(s)	702, 965, 2256, 2638
HphI	GGTGA	3 cut(s)	1195, 2041, 2407
Hpy166II	GTNNAC	6 cut(s)	997, 1312, 1727, 1976, 2101, 2457
Hpy8I	GTNNAC	6 cut(s)	997, 1312, 1727, 1976, 2101, 2457
Hpy99I	CGWCG	4 cut(s)	284, 329, 701, 2570
HpyAV	CCTTC	8 cut(s)	76, 463, 794, 1112, 1123, 1168, 2119, 2573
HpyCH4III	ACNGT	7 cut(s)	661, 729, 1571, 1731, 2051, 2150, 2169
HpyCH4IV	ACGT	2 cut(s)	240, 2451
HpySE526I	ACGT	2 cut(s)	240, 2451
Hsp92I	GRCGYC	3 cut(s)	282, 999, 2568
HspAI	GCGC	4 cut(s)	385, 558, 742, 2662
KroI	GCCGGC	1 cut(s)	701
KroNI	GCCGGC	1 cut(s)	703
Ksp22I	TGATCA	2 cut(s)	1323, 2058
KspAI	GTTAAC	1 cut(s)	2101
LmnI	GCTCC	6 cut(s)	56, 65, 1082, 1856, 2326, 2500
Lsp1109I	GCAGC	8 cut(s)	106, 144, 147, 187, 492, 1201, 1663, 1921
LweI	GCATC	6 cut(s)	73, 306, 808, 1791, 1896, 1978
MaeI	CTAG	4 cut(s)	23, 797, 1887, 2447
MaeII	ACGT	2 cut(s)	240, 2451
MaeIII	GTNAC	5 cut(s)	967, 1511, 1709, 2150, 2258
MbiI	CCGCTC	1 cut(s)	2329
MfeI	CAATTG	2 cut(s)	944, 2427
MflI	RGATCY	2 cut(s)	582, 1277
MhlI	GDGCHC	5 cut(s)	179, 1293, 2056, 2298, 2505
MlsI	TGGCCA	2 cut(s)	975, 1035
MluNI	TGGCCA	2 cut(s)	975, 1035
MlyI	GAGTC	1 cut(s)	1427
MmeI	TCCRAC	5 cut(s)	748, 1056, 1095, 1776, 2678
Mox20I	TGGCCA	2 cut(s)	975, 1035
Mph1103I	ATGCAT	1 cut(s)	1993
MroNI	GCCGGC	1 cut(s)	701
MroXI	GAANNNNTTC	3 cut(s)	1019, 2000, 2583
MscI	TGGCCA	2 cut(s)	975, 1035
MseI	TTAA	6 cut(s)	567, 1602, 1650, 2100, 2391, 2680
MslI	CAYNNNNRTG	1 cut(s)	2508
Msp20I	TGGCCA	2 cut(s)	975, 1035
MspA1I	CMGCKG	4 cut(s)	119, 144, 200, 1912
MspI	CCGG	4 cut(s)	702, 965, 2256, 2638
MspR9I	CCNGG	2 cut(s)	749, 1681
MunI	CAATTG	2 cut(s)	944, 2427
Mva1269I	GAATGC	3 cut(s)	796, 1216, 1378
MvaI	CCWGG	2 cut(s)	749, 1681
MvnI	CGCG	1 cut(s)	558
NaeI	GCCGGC	1 cut(s)	703
NcoI	CCATGG	1 cut(s)	213
NgoMIV	GCCGGC	1 cut(s)	701
NlaIV	GGNNCC	3 cut(s)	266, 1821, 2136
NmeAIII	GCCGAG	1 cut(s)	2426
NmuCI	GTSAC	3 cut(s)	967, 1511, 1709
NsiI	ATGCAT	1 cut(s)	1993
NspI	RCATGY	3 cut(s)	1031, 1586, 1868
NspV	TTCGAA	1 cut(s)	2520
OliI	CACNNNNGTG	1 cut(s)	2508
PaeI	GCATGC	2 cut(s)	1031, 1868
PaeR7I	CTCGAG	2 cut(s)	461, 1142
PceI	AGGCCT	1 cut(s)	452
PcsI	WCGNNNNNNNCGW	3 cut(s)	369, 483, 657
PctI	GAATGC	3 cut(s)	796, 1216, 1378
PdiI	GCCGGC	1 cut(s)	703
PdmI	GAANNNNTTC	3 cut(s)	1019, 2000, 2583
PfeI	GAWTC	2 cut(s)	764, 2413
PflFI	GACNNNGTC	1 cut(s)	242
PinAI	ACCGGT	1 cut(s)	2255
PleI	GAGTC	1 cut(s)	1427
PpsI	GAGTC	1 cut(s)	1427
PpuMI	RGGWCCY	2 cut(s)	466, 1057
PshBI	ATTAAT	1 cut(s)	1602
Psp124BI	GAGCTC	1 cut(s)	2298
Psp5II	RGGWCCY	2 cut(s)	466, 1057
Psp6I	CCWGG	2 cut(s)	747, 1679
PspFI	CCCAGC	5 cut(s)	539, 1067, 1238, 1823, 1859
PspGI	CCWGG	2 cut(s)	747, 1679
PspN4I	GGNNCC	3 cut(s)	266, 1821, 2136
PspPI	GGNCC	7 cut(s)	217, 244, 292, 418, 466, 1057, 1875
PspPPI	RGGWCCY	2 cut(s)	466, 1057
PspXI	VCTCGAGB	2 cut(s)	461, 1142
PstI	CTGCAG	1 cut(s)	2293
PstNI	CAGNNNCTG	1 cut(s)	2173
PsuI	RGATCY	2 cut(s)	582, 1277
PsyI	GACNNNGTC	1 cut(s)	242
RsaI	GTAC	5 cut(s)	650, 1552, 1581, 2313, 2636
RsaNI	GTAC	5 cut(s)	649, 1551, 1580, 2312, 2635
RseI	CAYNNNNRTG	1 cut(s)	2508
SacI	GAGCTC	1 cut(s)	2298
SaqAI	TTAA	6 cut(s)	567, 1602, 1650, 2100, 2391, 2680
Sau96I	GGNCC	7 cut(s)	217, 244, 292, 418, 466, 1057, 1875
ScaI	AGTACT	1 cut(s)	1552
SchI	GAGTC	1 cut(s)	1427
ScrFI	CCNGG	2 cut(s)	749, 1681
SduI	GDGCHC	5 cut(s)	179, 1293, 2056, 2298, 2505
SfaNI	GCATC	6 cut(s)	73, 306, 808, 1791, 1896, 1978
SfcI	CTRYAG	1 cut(s)	2289
Sfr274I	CTCGAG	2 cut(s)	461, 1142
SfuI	TTCGAA	1 cut(s)	2520
SinI	GGWCC	4 cut(s)	244, 292, 466, 1057
SlaI	CTCGAG	2 cut(s)	461, 1142
SmiMI	CAYNNNNRTG	1 cut(s)	2508
SmlI	CTYRAG	7 cut(s)	172, 461, 509, 890, 1142, 1259, 1457
SmoI	CTYRAG	7 cut(s)	172, 461, 509, 890, 1142, 1259, 1457
SphI	GCATGC	2 cut(s)	1031, 1868
SseBI	AGGCCT	1 cut(s)	452
SspI	AATATT	1 cut(s)	1330
SspMI	CTAG	4 cut(s)	23, 797, 1887, 2447
SstI	GAGCTC	1 cut(s)	2298
StuI	AGGCCT	1 cut(s)	452
StyD4I	CCNGG	2 cut(s)	747, 1679
StyI	CCWWGG	4 cut(s)	33, 213, 591, 1060
TaaI	ACNGT	7 cut(s)	661, 729, 1571, 1731, 2051, 2150, 2169
TaiI	ACGT	2 cut(s)	243, 2454
TatI	WGTACW	1 cut(s)	1550
TfiI	GAWTC	2 cut(s)	764, 2413
Tru1I	TTAA	6 cut(s)	567, 1602, 1650, 2100, 2391, 2680
Tru9I	TTAA	6 cut(s)	567, 1602, 1650, 2100, 2391, 2680
TscAI	CASTG	3 cut(s)	976, 991, 2056
TseFI	GTSAC	3 cut(s)	967, 1511, 1709
TseI	GCWGC	8 cut(s)	119, 132, 135, 200, 480, 1214, 1676, 1909
Tsp45I	GTSAC	3 cut(s)	967, 1511, 1709
TspDTI	ATGAA	6 cut(s)	1187, 1223, 1481, 1522, 1837, 2132
TspGWI	ACGGA	2 cut(s)	461, 1869
TspRI	CASTG	3 cut(s)	976, 991, 2056
Tth111I	GACNNNGTC	1 cut(s)	242
VpaK11BI	GGWCC	4 cut(s)	244, 292, 466, 1057
VspI	ATTAAT	1 cut(s)	1602
XagI	CCTNNNNNAGG	3 cut(s)	446, 590, 1145
XapI	RAATTY	1 cut(s)	1980
XceI	RCATGY	3 cut(s)	1031, 1586, 1868
XhoI	CTCGAG	2 cut(s)	461, 1142
XmnI	GAANNNNTTC	3 cut(s)	1019, 2000, 2583
XspI	CTAG	4 cut(s)	23, 797, 1887, 2447
ZrmI	AGTACT	1 cut(s)	1552
Zsp2I	ATGCAT	1 cut(s)	1993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.