Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007971

Physical Location & Seq

Genomic Coordinates Forward (+)

3707666 .. 3710446

Gene Structure 2781 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g03380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2781 bp

ATGCTTATTTCACTCAGAAATCTAGCCAAATCCAAGGCCAATACCAACTGCTCCCCTTGCTCCTCCTCCGCCGCCATCCTCGATGCCAATTCTCTTCTCTCCACCGCCGCCGCGGCTGCTGCTACCACCAGCAGCAGCATCAGCGGTGGTTGTTATTGCTTTTGTTGTTCCTTCAGTTTCAGCCCCAACGCTTCCCCTGCCGCCGCTGCTTTCTCAACCATGGCCCGCATGACTCCGGCGAGGGACGTGGTCCTCTCTTTCAAAGAATGGTTCCAGACCCGCAACGACGCCTTATTGGACCGCATCTTCCTCATCCTCAAATCCTCTACCGACGGCGACAGCGCCACCACCACCGACCGCCGCCTCAACGGCGACAGCGACATCCCCTTGCGCTATAATATCCCTTACTCCTCCGCCGACTTGGCCCTTTCCCATCTCAGTCTCCGCCTCACGGAGGCCTTCGTCCTCGAGGTCCTCCGCTACGGCAGCAACGACCATGATGTCCTCTCCTGCCTCAAGTTCTTTGATTGGGCCGGCCGCCAGCCCGGCTTCCACCACACCCGCGCCACCTTTAACGCCATCTTCAAGATCCTTTCCAAGGCCAAGCTCATGTCTCTCATGCTCGACTTCCTCTCCAACTACCGCAAGCAGAGGTACGCCCACACCGTCCGATTTCACGACACTCTGGTCATGGGCTACGCCGTCGCCGGCAAGCCCCACATTGCCCTCCAACTGTTTGGTAAAATGCGCTTCCAGGGTCTCGACCTCGATTCCTTTGCCTACCATGTCCTTTTGAATGCTCTAGTGGAGGAGAGTTGCTTTGATGCTGTTCAGGTCATTGCCAAGCAGATTTCTTTGAGGGGTTTCGAGAACGAAATTACCCATTCCATCATGCTCAAGTGGTTCTGTAAGCAGAAGTTGCTCGATCAAGCTGAAGCCTATTTGCGTCAATTGGTGTCTGACGGCAAGCCAGTCACTGGCCATGCCGTCAGTGTCCTTGTGGACGCCCTTTGTAAGCAGAAGAAGTTCCAGCATGCTGGCCAATTAGTCGAGGAGTTTCAGGACCTTGGGGTGGCTTTCATGGAGAGTGTTTATAGTGTGTGGATGCGTGATCTTGTTCAGGCTGGGAGGTTGGATGGAGCATTGGAGTTTTTGCAGAACAAGAAGTCGTTGGAAGGGTATGTACCTGATGCTTTTAGGTACAATACTTTGATATGTAGGCTTTTGAAGGAGGATCGCCTCGAGGAGGTTTGTGATTTGTTGATGGAGATGAAGGAGGGTAAGATTTCCCCTGATAAGATCACCATGAATGTTGCCTTGTGCTTCTTCTGCAAAGCGGGGATGGTGGATGTCGCACTTGAGTTGTATAATTCAAGATCCGAGTTTGGGCTCACGCCTAATAGTATGGTTTACAACTATTTGATCAATGTTTTCTGTGGGGATGGTAGCATTGACGAAGCCTATCGAGTGTTGAAGCATTCTATGGAACAAGGGTATTTTACAGGTAGGAAAACATTTTCTATTCTGGCAGACTCATTGTGCCGAGAGGGAAAGCTCGAGAAGATGAAAGAGTTGGTTATTTTCGCCCTAGAACGAAACTTTATGCCAAGTGACTATACATTTGATAAGTTCATTACAACTCTTTGCAGTACTGAGAGGGTAGAAGACGGTTATTTGGTACATGCGGAACTCAACAGAATTAATAAGGTTTCTACAAAGAAGACATATTTTAGCTTGATAAATGGTTTTAACAGGTCCAGCAAGGGGGATATTGCTGCCAGGCTTCTGATTGAAATGCAAGAAAAGGGTCACTTACCAATTCGTAAAGTGTTCAAAGATGTAATTTGCTGTCTCTGTGATATGGAAAATCCAGATAAGCAATTTTTTACTTTGTTGGAGATGCAGTTGTCTTGTAGGGAACCCAGCCGTGATATATACAACTTTTTCATCTACGGAGCTGGGCATGCCAAAAGGCCCGATCTGGCTAGACAAGTATTTGAGATGATGGAGCGGAGTGGGATTCATCCCAATGTGAGATCAGATGTTCTTATGTTGCAGGGTTATTTGAAGAGTGAACGAATTTCGGATGCATTGAACTTCTTCAATGATCTGCACCAGAGAAGAAAGGTGATGGGGAGAAGGCTATACAGCACCCTGATCGTTGGTCTATGCAAAGCCAAAAAGGCGGAAATTGCAGTTAACTTCTTGATGGAAATGAAAGAGAAGGGAGTGGTTCCAAGCGATGACTGTTACGAGTTTCTGATACAGTTGCTGTGCCAGAACAAAGAATTTGATATGGCGGTAAATCTCATAAATGACTTGGAGAAAGTTGGCCGCCATATTACATCCTTTACTGGTAACATACTTCTGTTGCAATCTCTGAAAACTCCAGAGCTCTACAAGTCTTGGGTACGGCTGAGAGAGGAGCAGAATGAGATGTCTGATTGTTCAATGCTTGGTCTGCTCATTGGTGCGTTTTCTGGTCAGATTAAGGTGAGCCGAGATATTGGGAATTTGGAAGAAGCAATTGAAATGTGCTTTCCACTAGACGTGTTCACACACAATTTGTTGATTAGAAGACTAAGCCAAAGCAACATGGAGCACGCTTGTGCTTTGTTCGAAAAGATGTGCCAGAAAGGTTATGAGCCTAATCGATGGACTTACGATGCCTTGGTAGAGGGTTTTCTTAGACATGGGAGAACATCTGAGGCAAAGAGATGGGTAGAAGTGATGTACCGGAAAGGGTTTTATCCAACAGGGCGCACAAATCTACTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

927

Amino Acids

105.29

Weight (kDa)

8.1

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	396 - 444	2.8e-09	PPR repeat family
PPR_2	PF13041	434 - 479	1.1e-07	PPR repeat family
PPR_1	PF12854	463 - 492	1.9e-06	PPR repeat
PPR_2	PF13041	466 - 515	2.9e-06	PPR repeat family
PPR_2	PF13041	716 - 760	1.9e-08	PPR repeat family
PPR_2	PF13041	850 - 896	2.4e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71210
fragaria_vesca	FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690
malus_domestica	MD05G1041400.v1.1 MD10G1047800.v1.1
prunus_persica	Prupe.8G057300_v2.0.a1 Prupe.8G057300_v2.0.a1
pyrus_communis	pycom05g03410 pycom10g03380 pycom17g22250
rosa_chinensis	RchiOBHm_Chr5g0055041
rosa_laevigata	RLG00000034959
rosa_multiflora	Rmu_sc0000925.1_g000011
rosa_roxburghii	Rroxscaffold_1G00025450
rosa_rugosa	Rorug05G0290400
rosa_samantha	Rh5AG360300 Rh5BG371300 Rh5CG393600 Rh5DG385100
rosa_wichuraiana	Rw5G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	500, 686
AbsI	CCTCGAGG	2 cut(s)	467, 1241
AccB7I	CCANNNNNTGG	1 cut(s)	977
AccBSI	CCGCTC	1 cut(s)	2011
AccII	CGCG	2 cut(s)	113, 564
AclWI	GGATC	3 cut(s)	583, 1242, 1371
AcoI	YGGCCR	4 cut(s)	535, 979, 1039, 2332
AcsI	RAATTY	3 cut(s)	2079, 2288, 2512
AcuI	CTGAAG	2 cut(s)	157, 954
AcyI	GRCGYC	2 cut(s)	288, 1005
AfaI	GTAC	7 cut(s)	656, 1185, 1202, 1651, 1680, 2412, 2735
AfiI	CCNNNNNNNGG	8 cut(s)	451, 454, 598, 977, 1072, 1246, 1386, 1764
AflIII	ACRYGT	1 cut(s)	2550
AjiI	CACGTC	2 cut(s)	247, 2551
AjnI	CCWGG	2 cut(s)	753, 1778
AleI	CACNNNNGTG	1 cut(s)	2607
AluBI	AGCT	6 cut(s)	607, 932, 1555, 1734, 1958, 2395
AluI	AGCT	6 cut(s)	607, 932, 1555, 1734, 1958, 2395
Alw21I	GWGCWC	2 cut(s)	2397, 2604
Alw26I	GTCTC	4 cut(s)	446, 618, 764, 1856
AlwI	GGATC	3 cut(s)	583, 1242, 1371
AlwNI	CAGNNNCTG	2 cut(s)	977, 2272
Ama87I	CYCGRG	3 cut(s)	467, 1241, 1556
ApeKI	GCWGC	7 cut(s)	116, 119, 132, 135, 206, 486, 1775
ApoI	RAATTY	3 cut(s)	2079, 2288, 2512
ArsI	GACNNNNNNTTYG	2 cut(s)	1604, 1636
AseI	ATTAAT	1 cut(s)	1701
Asp700I	GAANNNNTTC	2 cut(s)	1025, 2099
AspLEI	GCGC	5 cut(s)	344, 393, 566, 750, 2763
AspS9I	GGNCC	9 cut(s)	223, 250, 298, 424, 472, 531, 1063, 1755, 1974
AsuC2I	CCSGG	1 cut(s)	546
AsuHPI	GGTGA	3 cut(s)	1294, 2140, 2506
AsuII	TTCGAA	1 cut(s)	2619
AvaI	CYCGRG	3 cut(s)	467, 1241, 1556
AvaII	GGWCC	5 cut(s)	250, 298, 472, 1063, 1755
BalI	TGGCCA	2 cut(s)	981, 1041
BanII	GRGCYC	2 cut(s)	1392, 2397
BarI	GAAGNNNNNNTAC	2 cut(s)	1167, 1199
BbsI	GAAGAC	3 cut(s)	1671, 1727, 2584
Bbv12I	GWGCWC	2 cut(s)	2397, 2604
BbvI	GCAGC	7 cut(s)	103, 106, 144, 147, 193, 498, 1762
BceAI	ACGGC	8 cut(s)	349, 385, 499, 686, 971, 979, 1911, 2429
BcgI	CGANNNNNNTGC	4 cut(s)	758, 792, 2140, 2174
BciT130I	CCWGG	2 cut(s)	755, 1780
BclI	TGATCA	1 cut(s)	1422
BcnI	CCSGG	1 cut(s)	546
BcoDI	GTCTC	4 cut(s)	446, 618, 764, 1856
BfaI	CTAG	5 cut(s)	23, 803, 1589, 1986, 2546
BfoI	RGCGCY	1 cut(s)	345
BglI	GCCNNNNNGGC	5 cut(s)	113, 369, 422, 546, 2183
BmcAI	AGTACT	1 cut(s)	1651
Bme1390I	CCNGG	3 cut(s)	546, 755, 1780
Bme18I	GGWCC	5 cut(s)	250, 298, 472, 1063, 1755
BmeT110I	CYCGRG	3 cut(s)	467, 1241, 1556
BmgBI	CACGTC	2 cut(s)	247, 2551
BmgT120I	GGNCC	9 cut(s)	223, 250, 298, 424, 472, 531, 1063, 1755, 1974
BmiI	GGNNCC	3 cut(s)	272, 1920, 2235
BmrFI	CCNGG	3 cut(s)	546, 755, 1780
BmsI	GCATC	9 cut(s)	73, 147, 312, 814, 1095, 1180, 1890, 2077, 2656
BpiI	GAAGAC	3 cut(s)	1671, 1727, 2584
BpmI	CTGGAG	1 cut(s)	2373
Bpu14I	TTCGAA	1 cut(s)	2619
BpuEI	CTTGAG	3 cut(s)	500, 881, 1379
BpuMI	CCSGG	1 cut(s)	546
Bsa29I	ATCGAT	1 cut(s)	2653
BsaHI	GRCGYC	2 cut(s)	288, 1005
BsaI	GGTCTC	1 cut(s)	764
BsaJI	CCNNGG	7 cut(s)	33, 111, 219, 597, 754, 1066, 2670
BsaWI	WCCGGW	1 cut(s)	2736
BsaXI	ACNNNNNCTCC	4 cut(s)	617, 647, 1889, 1919
Bsc4I	CCNNNNNNNGG	8 cut(s)	451, 454, 598, 977, 1072, 1246, 1386, 1764
Bse118I	RCCGGY	2 cut(s)	533, 707
Bse1I	ACTGG	3 cut(s)	971, 982, 2359
Bse3DI	GCAATG	2 cut(s)	720, 837
BseBI	CCWGG	2 cut(s)	755, 1780
BseCI	ATCGAT	1 cut(s)	2653
BseDI	CCNNGG	7 cut(s)	33, 111, 219, 597, 754, 1066, 2670
BseLI	CCNNNNNNNGG	8 cut(s)	451, 454, 598, 977, 1072, 1246, 1386, 1764
BseMI	GCAATG	2 cut(s)	720, 837
BseMII	CTCAG	5 cut(s)	28, 451, 1644, 2408, 2697
BseNI	ACTGG	3 cut(s)	971, 982, 2359
BseRI	GAGGAG	7 cut(s)	52, 55, 400, 824, 1067, 1259, 2438
BseX3I	CGGCCG	1 cut(s)	535
BseXI	GCAGC	7 cut(s)	103, 106, 144, 147, 193, 498, 1762
BseYI	CCCAGC	3 cut(s)	1124, 1922, 1958
BsgI	GTGCAG	1 cut(s)	2096
Bsh1236I	CGCG	2 cut(s)	113, 564
Bsh1285I	CGRYCG	2 cut(s)	358, 538
BshVI	ATCGAT	1 cut(s)	2653
BsiEI	CGRYCG	2 cut(s)	358, 538
BsiHKAI	GWGCWC	2 cut(s)	2397, 2604
BsiHKCI	CYCGRG	3 cut(s)	467, 1241, 1556
BsiSI	CCGG	5 cut(s)	236, 534, 546, 708, 2737
BslFI	GGGAC	1 cut(s)	257
BslI	CCNNNNNNNGG	8 cut(s)	451, 454, 598, 977, 1072, 1246, 1386, 1764
BsmAI	GTCTC	4 cut(s)	446, 618, 764, 1856
BsmFI	GGGAC	1 cut(s)	257
BsmI	GAATGC	2 cut(s)	802, 1477
Bso31I	GGTCTC	1 cut(s)	764
BsoBI	CYCGRG	3 cut(s)	467, 1241, 1556
Bsp119I	TTCGAA	1 cut(s)	2619
Bsp1286I	GDGCHC	3 cut(s)	1392, 2397, 2604
Bsp19I	CCATGG	1 cut(s)	219
BspCNI	CTCAG	5 cut(s)	27, 450, 1645, 2409, 2698
BspDI	ATCGAT	1 cut(s)	2653
BspFNI	CGCG	2 cut(s)	113, 564
BspLI	GGNNCC	3 cut(s)	272, 1920, 2235
BspPI	GGATC	3 cut(s)	583, 1242, 1371
BspT104I	TTCGAA	1 cut(s)	2619
BspTNI	GGTCTC	1 cut(s)	764
BsrBI	CCGCTC	1 cut(s)	2011
BsrDI	GCAATG	2 cut(s)	720, 837
BsrFI	RCCGGY	2 cut(s)	533, 707
BsrI	ACTGG	3 cut(s)	971, 982, 2359
BssAI	RCCGGY	2 cut(s)	533, 707
BssECI	CCNNGG	7 cut(s)	33, 111, 219, 597, 754, 1066, 2670
BssNI	GRCGYC	2 cut(s)	288, 1005
BssT1I	CCWWGG	5 cut(s)	33, 219, 597, 1066, 2670
Bst2UI	CCWGG	2 cut(s)	755, 1780
Bst4CI	ACNGT	5 cut(s)	667, 735, 1670, 2249, 2268
Bst6I	CTCTTC	2 cut(s)	99, 2063
BstACI	GRCGYC	2 cut(s)	288, 1005
BstAPI	GCANNNNNTGC	1 cut(s)	919
BstBI	TTCGAA	1 cut(s)	2619
BstDEI	CTNAG	7 cut(s)	14, 437, 1653, 2417, 2582, 2687, 2706
BstDSI	CCRYGG	2 cut(s)	111, 219
BstENI	CCTNNNNNAGG	3 cut(s)	452, 596, 1244
BstFNI	CGCG	2 cut(s)	113, 564
BstH2I	RGCGCY	1 cut(s)	345
BstHHI	GCGC	5 cut(s)	344, 393, 566, 750, 2763
BstMAI	GTCTC	4 cut(s)	446, 618, 764, 1856
BstMCI	CGRYCG	2 cut(s)	358, 538
BstNI	CCWGG	2 cut(s)	755, 1780
BstNSI	RCATGY	3 cut(s)	1037, 1685, 1967
BstSCI	CCNGG	3 cut(s)	544, 753, 1778
BstUI	CGCG	2 cut(s)	113, 564
BstV1I	GCAGC	7 cut(s)	103, 106, 144, 147, 193, 498, 1762
BstV2I	GAAGAC	3 cut(s)	1671, 1727, 2584
BstX2I	RGATCY	2 cut(s)	588, 1376
BstXI	CCANNNNNNTGG	2 cut(s)	737, 1037
BstYI	RGATCY	2 cut(s)	588, 1376
BstZI	CGGCCG	1 cut(s)	535
Bsu15I	ATCGAT	1 cut(s)	2653
BsuTUI	ATCGAT	1 cut(s)	2653
BtgI	CCRYGG	2 cut(s)	111, 219
BtgZI	GCGATG	1 cut(s)	2256
BtrI	CACGTC	2 cut(s)	247, 2551
BtsIMutI	CAGTG	2 cut(s)	975, 997
CaiI	CAGNNNCTG	2 cut(s)	977, 2272
CfoI	GCGC	5 cut(s)	344, 393, 566, 750, 2763
Cfr10I	RCCGGY	2 cut(s)	533, 707
Cfr13I	GGNCC	9 cut(s)	223, 250, 298, 424, 472, 531, 1063, 1755, 1974
Cfr42I	CCGCGG	1 cut(s)	114
ClaI	ATCGAT	1 cut(s)	2653
CseI	GACGC	3 cut(s)	296, 935, 1013
Csp6I	GTAC	7 cut(s)	655, 1184, 1201, 1650, 1679, 2411, 2734
CviQI	GTAC	7 cut(s)	655, 1184, 1201, 1650, 1679, 2411, 2734
DdeI	CTNAG	7 cut(s)	14, 437, 1653, 2417, 2582, 2687, 2706
DrdI	GACNNNNNNGTC	2 cut(s)	500, 686
DseDI	GACNNNNNNGTC	2 cut(s)	500, 686
EaeI	YGGCCR	4 cut(s)	535, 979, 1039, 2332
EagI	CGGCCG	1 cut(s)	535
Eam1104I	CTCTTC	2 cut(s)	99, 2063
EarI	CTCTTC	2 cut(s)	99, 2063
EciI	GGCGGA	4 cut(s)	58, 403, 434, 2201
Ecl136II	GAGCTC	1 cut(s)	2395
EclXI	CGGCCG	1 cut(s)	535
Eco130I	CCWWGG	5 cut(s)	33, 219, 597, 1066, 2670
Eco147I	AGGCCT	1 cut(s)	458
Eco24I	GRGCYC	2 cut(s)	1392, 2397
Eco31I	GGTCTC	1 cut(s)	764
Eco47I	GGWCC	5 cut(s)	250, 298, 472, 1063, 1755
Eco52I	CGGCCG	1 cut(s)	535
Eco53kI	GAGCTC	1 cut(s)	2395
Eco57I	CTGAAG	2 cut(s)	157, 954
Eco88I	CYCGRG	3 cut(s)	467, 1241, 1556
EcoICRI	GAGCTC	1 cut(s)	2395
EcoNI	CCTNNNNNAGG	3 cut(s)	452, 596, 1244
EcoO109I	RGGNCCY	2 cut(s)	472, 1063
EcoRII	CCWGG	2 cut(s)	753, 1778
EcoT14I	CCWWGG	5 cut(s)	33, 219, 597, 1066, 2670
EcoT22I	ATGCAT	1 cut(s)	2092
EcoT38I	GRGCYC	2 cut(s)	1392, 2397
ErhI	CCWWGG	5 cut(s)	33, 219, 597, 1066, 2670
FalI	AAGNNNNNCTT	2 cut(s)	1796, 1828
FaqI	GGGAC	1 cut(s)	257
FauI	CCCGC	4 cut(s)	233, 287, 569, 1330
FbaI	TGATCA	1 cut(s)	1422
FriOI	GRGCYC	2 cut(s)	1392, 2397
FseI	GGCCGGCC	1 cut(s)	537
FspBI	CTAG	5 cut(s)	23, 803, 1589, 1986, 2546
GlaI	GCGC	5 cut(s)	343, 392, 565, 749, 2762
GsaI	CCCAGC	3 cut(s)	1128, 1926, 1962
GsuI	CTGGAG	1 cut(s)	2373
HaeII	RGCGCY	1 cut(s)	345
HapII	CCGG	5 cut(s)	236, 534, 546, 708, 2737
HgaI	GACGC	3 cut(s)	296, 935, 1013
HhaI	GCGC	5 cut(s)	344, 393, 566, 750, 2763
Hin1I	GRCGYC	2 cut(s)	288, 1005
Hin6I	GCGC	5 cut(s)	342, 391, 564, 748, 2761
HinP1I	GCGC	5 cut(s)	342, 391, 564, 748, 2761
HincII	GTYRAC	1 cut(s)	2200
HindII	GTYRAC	1 cut(s)	2200
HinfI	GANTC	4 cut(s)	232, 770, 1532, 2020
HpaI	GTTAAC	1 cut(s)	2200
HpaII	CCGG	5 cut(s)	236, 534, 546, 708, 2737
HphI	GGTGA	3 cut(s)	1294, 2140, 2506
Hpy166II	GTNNAC	5 cut(s)	1003, 1411, 2075, 2200, 2556
Hpy8I	GTNNAC	5 cut(s)	1003, 1411, 2075, 2200, 2556
Hpy99I	CGWCG	3 cut(s)	290, 335, 707
HpyAV	CCTTC	7 cut(s)	181, 469, 1169, 1222, 1267, 2133, 2218
HpyCH4III	ACNGT	5 cut(s)	667, 735, 1670, 2249, 2268
HpyCH4IV	ACGT	2 cut(s)	246, 2550
HpyF3I	CTNAG	7 cut(s)	14, 437, 1653, 2417, 2582, 2687, 2706
HpySE526I	ACGT	2 cut(s)	246, 2550
Hsp92I	GRCGYC	2 cut(s)	288, 1005
HspAI	GCGC	5 cut(s)	342, 391, 564, 748, 2761
KroI	GCCGGC	2 cut(s)	533, 707
KroNI	GCCGGC	2 cut(s)	535, 709
Ksp22I	TGATCA	1 cut(s)	1422
KspAI	GTTAAC	1 cut(s)	2200
KspI	CCGCGG	1 cut(s)	114
LmnI	GCTCC	7 cut(s)	56, 65, 1139, 1955, 2008, 2425, 2599
Lsp1109I	GCAGC	7 cut(s)	103, 106, 144, 147, 193, 498, 1762
LweI	GCATC	9 cut(s)	73, 147, 312, 814, 1095, 1180, 1890, 2077, 2656
MaeI	CTAG	5 cut(s)	23, 803, 1589, 1986, 2546
MaeII	ACGT	2 cut(s)	246, 2550
MaeIII	GTNAC	5 cut(s)	973, 1610, 1808, 2249, 2357
MbiI	CCGCTC	1 cut(s)	2011
MfeI	CAATTG	2 cut(s)	950, 2526
MflI	RGATCY	2 cut(s)	588, 1376
MhlI	GDGCHC	3 cut(s)	1392, 2397, 2604
MlsI	TGGCCA	2 cut(s)	981, 1041
MluNI	TGGCCA	2 cut(s)	981, 1041
MlyI	GAGTC	2 cut(s)	226, 1526
MmeI	TCCRAC	6 cut(s)	660, 754, 1113, 1152, 1875, 2777
Mox20I	TGGCCA	2 cut(s)	981, 1041
Mph1103I	ATGCAT	1 cut(s)	2092
MroNI	GCCGGC	2 cut(s)	533, 707
MroXI	GAANNNNTTC	2 cut(s)	1025, 2099
MscI	TGGCCA	2 cut(s)	981, 1041
MseI	TTAA	6 cut(s)	573, 1701, 1749, 2199, 2490, 2779
MslI	CAYNNNNRTG	2 cut(s)	2028, 2607
Msp20I	TGGCCA	2 cut(s)	981, 1041
MspA1I	CMGCKG	3 cut(s)	113, 144, 206
MspI	CCGG	5 cut(s)	236, 534, 546, 708, 2737
MspR9I	CCNGG	3 cut(s)	546, 755, 1780
MunI	CAATTG	2 cut(s)	950, 2526
Mva1269I	GAATGC	2 cut(s)	802, 1477
MvaI	CCWGG	2 cut(s)	755, 1780
MvnI	CGCG	2 cut(s)	113, 564
NaeI	GCCGGC	2 cut(s)	535, 709
NciI	CCSGG	1 cut(s)	546
NcoI	CCATGG	1 cut(s)	219
NgoMIV	GCCGGC	2 cut(s)	533, 707
NlaIV	GGNNCC	3 cut(s)	272, 1920, 2235
NmeAIII	GCCGAG	2 cut(s)	1568, 2525
NmuCI	GTSAC	3 cut(s)	973, 1610, 1808
NsiI	ATGCAT	1 cut(s)	2092
NspI	RCATGY	3 cut(s)	1037, 1685, 1967
NspV	TTCGAA	1 cut(s)	2619
OliI	CACNNNNGTG	1 cut(s)	2607
PaeI	GCATGC	2 cut(s)	1037, 1967
PaeR7I	CTCGAG	3 cut(s)	467, 1241, 1556
PceI	AGGCCT	1 cut(s)	458
PcsI	WCGNNNNNNNCGW	3 cut(s)	375, 489, 663
PctI	GAATGC	2 cut(s)	802, 1477
PdiI	GCCGGC	2 cut(s)	535, 709
PdmI	GAANNNNTTC	2 cut(s)	1025, 2099
PfeI	GAWTC	2 cut(s)	770, 2020
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	1 cut(s)	977
PleI	GAGTC	2 cut(s)	226, 1526
PpsI	GAGTC	2 cut(s)	226, 1526
PpuMI	RGGWCCY	2 cut(s)	472, 1063
PshBI	ATTAAT	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	2397
Psp5II	RGGWCCY	2 cut(s)	472, 1063
Psp6I	CCWGG	2 cut(s)	753, 1778
PspFI	CCCAGC	3 cut(s)	1124, 1922, 1958
PspGI	CCWGG	2 cut(s)	753, 1778
PspN4I	GGNNCC	3 cut(s)	272, 1920, 2235
PspPI	GGNCC	9 cut(s)	223, 250, 298, 424, 472, 531, 1063, 1755, 1974
PspPPI	RGGWCCY	2 cut(s)	472, 1063
PspXI	VCTCGAGB	2 cut(s)	467, 1241
PstNI	CAGNNNCTG	2 cut(s)	977, 2272
PsuI	RGATCY	2 cut(s)	588, 1376
PsyI	GACNNNGTC	1 cut(s)	248
RigI	GGCCGGCC	1 cut(s)	537
RsaI	GTAC	7 cut(s)	656, 1185, 1202, 1651, 1680, 2412, 2735
RsaNI	GTAC	7 cut(s)	655, 1184, 1201, 1650, 1679, 2411, 2734
RseI	CAYNNNNRTG	2 cut(s)	2028, 2607
SacI	GAGCTC	1 cut(s)	2397
SacII	CCGCGG	1 cut(s)	114
SaqAI	TTAA	6 cut(s)	573, 1701, 1749, 2199, 2490, 2779
Sau96I	GGNCC	9 cut(s)	223, 250, 298, 424, 472, 531, 1063, 1755, 1974
ScaI	AGTACT	1 cut(s)	1651
SchI	GAGTC	2 cut(s)	226, 1526
ScrFI	CCNGG	3 cut(s)	546, 755, 1780
SduI	GDGCHC	3 cut(s)	1392, 2397, 2604
SfaNI	GCATC	9 cut(s)	73, 147, 312, 814, 1095, 1180, 1890, 2077, 2656
Sfr274I	CTCGAG	3 cut(s)	467, 1241, 1556
Sfr303I	CCGCGG	1 cut(s)	114
SfuI	TTCGAA	1 cut(s)	2619
SgrBI	CCGCGG	1 cut(s)	114
SinI	GGWCC	5 cut(s)	250, 298, 472, 1063, 1755
SlaI	CTCGAG	3 cut(s)	467, 1241, 1556
SmiMI	CAYNNNNRTG	2 cut(s)	2028, 2607
SmlI	CTYRAG	6 cut(s)	467, 515, 896, 1241, 1358, 1556
SmoI	CTYRAG	6 cut(s)	467, 515, 896, 1241, 1358, 1556
SphI	GCATGC	2 cut(s)	1037, 1967
SseBI	AGGCCT	1 cut(s)	458
SspMI	CTAG	5 cut(s)	23, 803, 1589, 1986, 2546
SstI	GAGCTC	1 cut(s)	2397
StuI	AGGCCT	1 cut(s)	458
StyD4I	CCNGG	3 cut(s)	544, 753, 1778
StyI	CCWWGG	5 cut(s)	33, 219, 597, 1066, 2670
TaaI	ACNGT	5 cut(s)	667, 735, 1670, 2249, 2268
TaiI	ACGT	2 cut(s)	249, 2553
TatI	WGTACW	1 cut(s)	1649
TauI	GCSGC	9 cut(s)	74, 110, 113, 116, 203, 206, 363, 540, 2337
TfiI	GAWTC	2 cut(s)	770, 2020
Tru1I	TTAA	6 cut(s)	573, 1701, 1749, 2199, 2490, 2779
Tru9I	TTAA	6 cut(s)	573, 1701, 1749, 2199, 2490, 2779
TscAI	CASTG	2 cut(s)	982, 997
TseFI	GTSAC	3 cut(s)	973, 1610, 1808
TseI	GCWGC	7 cut(s)	116, 119, 132, 135, 206, 486, 1775
Tsp45I	GTSAC	3 cut(s)	973, 1610, 1808
TspDTI	ATGAA	8 cut(s)	1069, 1286, 1322, 1580, 1621, 1936, 2012, 2231
TspGWI	ACGGA	2 cut(s)	467, 1968
TspRI	CASTG	2 cut(s)	982, 997
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	1 cut(s)	977
VpaK11BI	GGWCC	5 cut(s)	250, 298, 472, 1063, 1755
VspI	ATTAAT	1 cut(s)	1701
XagI	CCTNNNNNAGG	3 cut(s)	452, 596, 1244
XapI	RAATTY	3 cut(s)	2079, 2288, 2512
XceI	RCATGY	3 cut(s)	1037, 1685, 1967
XhoI	CTCGAG	3 cut(s)	467, 1241, 1556
XmnI	GAANNNNTTC	2 cut(s)	1025, 2099
XspI	CTAG	5 cut(s)	23, 803, 1589, 1986, 2546
ZrmI	AGTACT	1 cut(s)	1651
Zsp2I	ATGCAT	1 cut(s)	2092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.