Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007971

Physical Location & Seq

Genomic Coordinates Forward (+)

3898207 .. 3900894

Gene Structure 2688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGCTTATTTCACTCAGAAATCTAGCCAAATCCAAGGCCAATACCAACTGCTCCCTCTGCTGCTCCTCCACCGCCATCCTCGATGCCACTTTTCTTCTCACCACTACCGCCGCCGCTGCTGCCACCAGAACCAGCAGCATTAGCGGTTGTTGTTCCTTCAGTTTCGGCCCCAACGCTCCCCCTACCCCCGCTCCTTTTTCAACCTCAGCTCGCTTGACTCCGGCAAGGGACGTGGTCCTCTCTTTCAAAGAATGGTTCAACACCCGCAACGACGCCTTATTCGACCACATCTTCCTCATCCTCAAATCCTCCACCGACGACGACGTCTTGGCCCTCTCCCATCTCAGCGTCCGCCTCTCGGAGTCCTTCGTCCTCGAGGTCCTACGCTACGGCAGCAACGACCATGACGTCCTCTCCTGCCTCAAGTTCTTCGACTGGGCCGGCCGCCAGCCCGGCTTCCACCACACCCGCGCCACCTTCAACGCCATCTTCAAGATCCTTTCCAAGGCCAAGCTCATGTCCCTTATGCTCGACGTCCTCTCCACCTACAGCAAGCAGAGGTACGCCCACACCGTCCGATTTCACGACACGCTGGTCATGGGCTACGCTGTCGCCGGCAAGCCCGACATTGCTCTCCAACTGTTTGGTAAAATGCGCTTCCATGGTCTGGACCTGGATTCCTTTGCCTACCATGTCCTTTTGAATGCTTTGGTGGAGGAGAGTTGCTTTGATGCCGTTCAGGTCATTGCCAAGCAGATTTCTTTGAGGGGTTTTGAGAACGAAATCACCCATTCCATCATGCTCAAGTGGTTGTGTAAGCAGAAGTTGCTCGATCAAGCTGAAGCCTATTTGCGCCAATTGGTGTCTGACGGCAAGCCCGTCACCGGGCATGCAGTCAGTGTCCTTGTGGACGCCCTTTGTAAGCATAAGAAGTTCCAGCAGGCTGGTGAATTAGTCGAGGAGTTTCGGGACTTGGGGGTGGCTCTCATGGAGAGTGCTTATGGTGTGTGGATACGCGATCTTGTTCAGGCTGGGAGGTTGGATGGAGCATTGGAGTTTTTTCAGAAAAAGAAGTCGTTGGAAGGATATGTGCCCGATGCTTTTCGGTACAATACTTTGATATGTAGCCTTCTGAAAGAGGATCACCTTGAGGAGGTTTGTGATTTGCTGATGGAGATGAAGGAGAATAAGATATCCCCTGATAAGGTCACCATGAATGCTGCCTTGTGCTTCTTTTGCAAAGCGGGGATGGTGGATGTCGCACTTGAGTTATATAATTCGAGATCCGAGTTTCGGCTCACGCCTAATAGTATGATTTACAACTATTCGATCAATGTTTTCTGTGGGGAAGGTAGCATTGACGAAGCGTATCGAGTTATGAAGCATTCTGTGGAACAAGGTTATTTTACAGGAAGGAAAACGTTTTCTATTCTGGCAGACTCATTGTGCCGAGAGGGAAAGCTCGAGAAGATGAAAGAGTTGGTTACCTTCGCCCTAGAGCGGAACTTTATGCCAAGTAACTCCACATATGATAAGTTCATTACAACTCTTTGCAGAACTAAGAGGGTAGAAGACGCGTATTTGATACATGGGGAACTCAATAGAATTAACAAGGGTTCTACAAAGAGTAGATACTTTAGCTTGATAAATGGTTTTAACGGGTTGAGCAAGGGGGATATTGCTGCCAGACTTCTGATTGAAATGCAAGAAAAGGGTCACTTACCGACTCGCAAGCTGTTCAAAGATGTAATTTGTTGTCTATGTGATATGGAAAATCCAGATGAGCAATTTTTTAATTTGTTGGAGATGCAGTTGTCTTGTAGGGAACCCAGCTGTGATGTATACAACTTTTTCATCTACGGAGCTGGGTATGCCAAAAGGCCCGATCTGGCTAGACAAGTATATGAGATGATGCAGCGGAGTGGAATTCAGCCCAATTTGAGATCTGATGTTATTATGTTGCAGAGTTATTTGAAGGGTGAATGGATTTCGGATGCTTTGAACTTCTTCAATGATCTGCACGAGAGAAGAAAGGTGTTGGGGAGAAGGCTATACAGCGCCCTGATCGTTGGTCTATGCAAAGCCCAAAAGGCAGAAATTGCAGTTAACTTCTTGATGGAAATGAAAGAGAAGGGAGTGGTTCCAAGCGATGACTGTTACGAGTTTCTGATACAGTTGCTGTGCTGGAATAAAGAATTTGATATGGCAGTAAATCTCATAAATGACTTGGAGAAAGTTGGCCGCCATATTACATCCTTTACTGGTAACATACTTCTGTTGCATTCTCTGAAAACTCCAGAGCTCTACAATTCTTGGGTACGGCTGAGAGAGGAGCAGAATGAGATGTCTGGTTGTTCAATGCTTGGTCTGCTCATTGGTGCATTTTCTGGTCGTATTCAGGTGAGCCAAGATATTGGGAATTTGGAAGAAGTAATTGAAAAGTGCTTTCCGCTAGATGTGTTCACACACAATATGTTGATTAGAAGACTAAGCCAGAGCAACATGGAGCATGCTTGTGCTTTGTTCGATAAGATGTGCCAGAAAGGTTACGAGCCTAATCGTTGGACTTATGACGCCATAGTACAAGGTTTTCTTAAACATGGGAGGACATCTGAGGCAAAGAGATGGGTAGAAGTGATGTACCGGAAAGGGTTTTATCCAACAAGGCGCACAAATCTACTCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

896

Amino Acids

101.88

Weight (kDa)

7.85

Isoelectric Point (pI)

35.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	170 - 318	5.5e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	365 - 407	1.4e-09	PPR repeat family
PPR_2	PF13041	685 - 728	2.6e-07	PPR repeat family
PPR_2	PF13041	819 - 864	2.3e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71210
fragaria_vesca	FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690
malus_domestica	MD05G1041400.v1.1 MD10G1047800.v1.1
prunus_persica	Prupe.8G057300_v2.0.a1 Prupe.8G057300_v2.0.a1
pyrus_communis	pycom05g03410 pycom10g03380 pycom17g22250
rosa_chinensis	RchiOBHm_Chr5g0055041
rosa_laevigata	RLG00000034959
rosa_multiflora	Rmu_sc0000925.1_g000011
rosa_roxburghii	Rroxscaffold_1G00025450
rosa_rugosa	Rorug05G0290400
rosa_samantha	Rh5AG360300 Rh5BG371300 Rh5CG393600 Rh5DG385100
rosa_wichuraiana	Rw5G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	323, 407, 593
AatII	GACGTC	3 cut(s)	327, 411, 537
AbsI	CCTCGAGG	1 cut(s)	374
AccB7I	CCANNNNNTGG	1 cut(s)	667
AccBSI	CCGCTC	2 cut(s)	191, 1501
AccI	GTMKAC	1 cut(s)	1842
AccII	CGCG	3 cut(s)	471, 1017, 1577
AclI	AACGTT	1 cut(s)	1421
AclWI	GGATC	3 cut(s)	490, 1149, 1278
AcoI	YGGCCR	2 cut(s)	442, 2239
AcsI	RAATTY	3 cut(s)	1926, 2195, 2419
AcuI	CTGAAG	2 cut(s)	142, 861
AcyI	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
AfaI	GTAC	5 cut(s)	563, 1109, 2319, 2583, 2642
AfiI	CCNNNNNNNGG	9 cut(s)	358, 505, 667, 884, 885, 1153, 1204, 1293, 1500
AflIII	ACRYGT	1 cut(s)	1575
AjiI	CACGTC	1 cut(s)	232
AjnI	CCWGG	1 cut(s)	672
AluBI	AGCT	9 cut(s)	209, 514, 839, 1462, 1641, 1735, 1833, 1865, 2302
AluI	AGCT	9 cut(s)	209, 514, 839, 1462, 1641, 1735, 1833, 1865, 2302
Alw21I	GWGCWC	1 cut(s)	2304
AlwI	GGATC	3 cut(s)	490, 1149, 1278
AlwNI	CAGNNNCTG	1 cut(s)	2179
Ama87I	CYCGRG	2 cut(s)	374, 1463
AoxI	GGCC	8 cut(s)	36, 166, 330, 438, 442, 507, 1880, 2239
ApeKI	GCWGC	8 cut(s)	60, 116, 119, 135, 393, 1220, 1682, 1915
ApoI	RAATTY	3 cut(s)	1926, 2195, 2419
ArsI	GACNNNNNNTTYG	2 cut(s)	263, 295
Asp700I	GAANNNNTTC	1 cut(s)	2006
AspLEI	GCGC	5 cut(s)	473, 657, 855, 2060, 2670
AspS9I	GGNCC	7 cut(s)	167, 235, 331, 379, 438, 670, 1881
AsuC2I	CCSGG	2 cut(s)	453, 886
AsuHPI	GGTGA	8 cut(s)	91, 778, 874, 959, 1136, 1201, 1991, 2413
AvaI	CYCGRG	2 cut(s)	374, 1463
AvaII	GGWCC	3 cut(s)	235, 379, 670
BaeGI	GKGCMC	1 cut(s)	1095
BanII	GRGCYC	1 cut(s)	2304
BauI	CACGAG	1 cut(s)	2021
BbsI	GAAGAC	2 cut(s)	1578, 2491
Bbv12I	GWGCWC	1 cut(s)	2304
BbvCI	CCTCAGC	1 cut(s)	205
BbvI	GCAGC	8 cut(s)	47, 103, 106, 147, 405, 1207, 1669, 1927
BccI	CCATC	9 cut(s)	83, 348, 494, 803, 1037, 1165, 1243, 2110, 2619
BceAI	ACGGC	4 cut(s)	406, 719, 886, 2336
BciT130I	CCWGG	1 cut(s)	674
BciVI	GTATCC	1 cut(s)	1005
BcnI	CCSGG	2 cut(s)	453, 886
BfaI	CTAG	4 cut(s)	23, 1496, 1893, 2453
BfmI	CTRYAG	1 cut(s)	547
BfoI	RGCGCY	1 cut(s)	2061
BfuI	GTATCC	1 cut(s)	1005
BglI	GCCNNNNNGGC	2 cut(s)	453, 2090
BglII	AGATCT	1 cut(s)	1943
Bme1390I	CCNGG	3 cut(s)	453, 674, 886
Bme18I	GGWCC	3 cut(s)	235, 379, 670
BmeT110I	CYCGRG	2 cut(s)	374, 1463
BmgBI	CACGTC	1 cut(s)	232
BmgT120I	GGNCC	7 cut(s)	167, 235, 331, 379, 438, 670, 1881
BmiI	GGNNCC	3 cut(s)	169, 1827, 2142
BmrFI	CCNGG	3 cut(s)	453, 674, 886
BmrI	ACTGGG	1 cut(s)	445
BmsI	GCATC	6 cut(s)	73, 721, 1087, 1797, 1902, 1984
BmuI	ACTGGG	1 cut(s)	445
BpiI	GAAGAC	2 cut(s)	1578, 2491
BpmI	CTGGAG	1 cut(s)	2280
Bpu10I	CCTNAGC	1 cut(s)	205
BpuEI	CTTGAG	4 cut(s)	407, 788, 1169, 1286
BpuMI	CCSGG	2 cut(s)	453, 886
BsaHI	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
BsaJI	CCNNGG	3 cut(s)	33, 504, 661
BsaWI	WCCGGW	1 cut(s)	2643
BsaXI	ACNNNNNCTCC	8 cut(s)	175, 205, 398, 428, 524, 554, 1796, 1826
Bsc4I	CCNNNNNNNGG	9 cut(s)	358, 505, 667, 884, 885, 1153, 1204, 1293, 1500
Bse118I	RCCGGY	2 cut(s)	440, 614
Bse1I	ACTGG	2 cut(s)	440, 2266
Bse3DI	GCAATG	2 cut(s)	627, 744
BseBI	CCWGG	1 cut(s)	674
BseDI	CCNNGG	3 cut(s)	33, 504, 661
BseGI	GGATG	7 cut(s)	75, 297, 1048, 1254, 1261, 1999, 2252
BseLI	CCNNNNNNNGG	9 cut(s)	358, 505, 667, 884, 885, 1153, 1204, 1293, 1500
BseMI	GCAATG	2 cut(s)	627, 744
BseMII	CTCAG	5 cut(s)	28, 219, 358, 2315, 2604
BseNI	ACTGG	2 cut(s)	440, 2266
BseRI	GAGGAG	5 cut(s)	55, 731, 974, 1166, 2345
BseSI	GKGCMC	1 cut(s)	1095
BseX3I	CGGCCG	1 cut(s)	442
BseXI	GCAGC	8 cut(s)	47, 103, 106, 147, 405, 1207, 1669, 1927
BseYI	CCCAGC	3 cut(s)	1031, 1829, 1865
BsgI	GTGCAG	1 cut(s)	2003
Bsh1236I	CGCG	3 cut(s)	471, 1017, 1577
Bsh1285I	CGRYCG	1 cut(s)	445
BshFI	GGCC	8 cut(s)	38, 168, 332, 440, 444, 509, 1882, 2241
BsiEI	CGRYCG	1 cut(s)	445
BsiHKAI	GWGCWC	1 cut(s)	2304
BsiHKCI	CYCGRG	2 cut(s)	374, 1463
BsiSI	CCGG	6 cut(s)	221, 441, 453, 615, 885, 2644
BslFI	GGGAC	3 cut(s)	242, 505, 983
BslI	CCNNNNNNNGG	9 cut(s)	358, 505, 667, 884, 885, 1153, 1204, 1293, 1500
BsmFI	GGGAC	3 cut(s)	242, 505, 983
BsmI	GAATGC	4 cut(s)	709, 1222, 1384, 2281
BsnI	GGCC	8 cut(s)	38, 168, 332, 440, 444, 509, 1882, 2241
BsoBI	CYCGRG	2 cut(s)	374, 1463
Bsp1286I	GDGCHC	2 cut(s)	1095, 2304
Bsp19I	CCATGG	1 cut(s)	661
BspANI	GGCC	8 cut(s)	38, 168, 332, 440, 444, 509, 1882, 2241
BspCNI	CTCAG	5 cut(s)	27, 218, 357, 2316, 2605
BspFNI	CGCG	3 cut(s)	471, 1017, 1577
BspLI	GGNNCC	3 cut(s)	169, 1827, 2142
BspPI	GGATC	3 cut(s)	490, 1149, 1278
BsrBI	CCGCTC	2 cut(s)	191, 1501
BsrDI	GCAATG	2 cut(s)	627, 744
BsrFI	RCCGGY	2 cut(s)	440, 614
BsrI	ACTGG	2 cut(s)	440, 2266
BssAI	RCCGGY	2 cut(s)	440, 614
BssECI	CCNNGG	3 cut(s)	33, 504, 661
BssNAI	GTATAC	1 cut(s)	1843
BssNI	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
BssSI	CACGAG	1 cut(s)	2021
BssT1I	CCWWGG	3 cut(s)	33, 504, 661
Bst1107I	GTATAC	1 cut(s)	1843
Bst2BI	CACGAG	1 cut(s)	2021
Bst2UI	CCWGG	1 cut(s)	674
Bst4CI	ACNGT	4 cut(s)	574, 642, 2156, 2175
BstACI	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
BstAPI	GCANNNNNTGC	2 cut(s)	826, 2099
BstDEI	CTNAG	7 cut(s)	14, 205, 344, 1560, 2324, 2489, 2613
BstDSI	CCRYGG	1 cut(s)	661
BstEII	GGTNACC	2 cut(s)	1207, 1483
BstENI	CCTNNNNNAGG	2 cut(s)	503, 1151
BstF5I	GGATG	7 cut(s)	75, 297, 1048, 1254, 1261, 1999, 2252
BstFNI	CGCG	3 cut(s)	471, 1017, 1577
BstH2I	RGCGCY	1 cut(s)	2061
BstHHI	GCGC	5 cut(s)	473, 657, 855, 2060, 2670
BstMCI	CGRYCG	1 cut(s)	445
BstNI	CCWGG	1 cut(s)	674
BstNSI	RCATGY	2 cut(s)	893, 2513
BstPI	GGTNACC	2 cut(s)	1207, 1483
BstSCI	CCNGG	3 cut(s)	451, 672, 884
BstSFI	CTRYAG	1 cut(s)	547
BstSLI	GKGCMC	1 cut(s)	1095
BstUI	CGCG	3 cut(s)	471, 1017, 1577
BstV1I	GCAGC	8 cut(s)	47, 103, 106, 147, 405, 1207, 1669, 1927
BstV2I	GAAGAC	2 cut(s)	1578, 2491
BstX2I	RGATCY	3 cut(s)	495, 1283, 1943
BstXI	CCANNNNNNTGG	3 cut(s)	644, 944, 2414
BstYI	RGATCY	3 cut(s)	495, 1283, 1943
BstZ17I	GTATAC	1 cut(s)	1843
BstZI	CGGCCG	1 cut(s)	442
BsuI	GTATCC	1 cut(s)	1005
BsuRI	GGCC	8 cut(s)	38, 168, 332, 440, 444, 509, 1882, 2241
BtgI	CCRYGG	1 cut(s)	661
BtgZI	GCGATG	1 cut(s)	2163
BtrI	CACGTC	1 cut(s)	232
BtsCI	GGATG	7 cut(s)	75, 297, 1048, 1254, 1261, 1999, 2252
BtsIMutI	CAGTG	1 cut(s)	904
CaiI	CAGNNNCTG	1 cut(s)	2179
CfoI	GCGC	5 cut(s)	473, 657, 855, 2060, 2670
Cfr10I	RCCGGY	2 cut(s)	440, 614
Cfr13I	GGNCC	7 cut(s)	167, 235, 331, 379, 438, 670, 1881
CseI	GACGC	5 cut(s)	281, 337, 920, 1583, 2582
Csp6I	GTAC	5 cut(s)	562, 1108, 2318, 2582, 2641
CspCI	CAANNNNNGTGG	2 cut(s)	213, 248
CviQI	GTAC	5 cut(s)	562, 1108, 2318, 2582, 2641
DdeI	CTNAG	7 cut(s)	14, 205, 344, 1560, 2324, 2489, 2613
DrdI	GACNNNNNNGTC	3 cut(s)	323, 407, 593
DseDI	GACNNNNNNGTC	3 cut(s)	323, 407, 593
EaeI	YGGCCR	2 cut(s)	442, 2239
EagI	CGGCCG	1 cut(s)	442
EciI	GGCGGA	1 cut(s)	341
Ecl136II	GAGCTC	1 cut(s)	2302
EclXI	CGGCCG	1 cut(s)	442
Eco130I	CCWWGG	3 cut(s)	33, 504, 661
Eco24I	GRGCYC	1 cut(s)	2304
Eco32I	GATATC	1 cut(s)	1194
Eco47I	GGWCC	3 cut(s)	235, 379, 670
Eco52I	CGGCCG	1 cut(s)	442
Eco53kI	GAGCTC	1 cut(s)	2302
Eco57I	CTGAAG	2 cut(s)	142, 861
Eco88I	CYCGRG	2 cut(s)	374, 1463
Eco91I	GGTNACC	2 cut(s)	1207, 1483
EcoICRI	GAGCTC	1 cut(s)	2302
EcoNI	CCTNNNNNAGG	2 cut(s)	503, 1151
EcoO109I	RGGNCCY	1 cut(s)	379
EcoO65I	GGTNACC	2 cut(s)	1207, 1483
EcoRI	GAATTC	1 cut(s)	1926
EcoRII	CCWGG	1 cut(s)	672
EcoRV	GATATC	1 cut(s)	1194
EcoT14I	CCWWGG	3 cut(s)	33, 504, 661
EcoT38I	GRGCYC	1 cut(s)	2304
ErhI	CCWWGG	3 cut(s)	33, 504, 661
FalI	AAGNNNNNCTT	4 cut(s)	1703, 1735, 2577, 2609
FaqI	GGGAC	3 cut(s)	242, 505, 983
FauI	CCCGC	4 cut(s)	196, 272, 476, 1237
FauNDI	CATATG	1 cut(s)	1528
FblI	GTMKAC	1 cut(s)	1842
FokI	GGATG	7 cut(s)	62, 284, 1055, 1261, 1268, 2006, 2239
FriOI	GRGCYC	1 cut(s)	2304
FseI	GGCCGGCC	1 cut(s)	444
FspBI	CTAG	4 cut(s)	23, 1496, 1893, 2453
GlaI	GCGC	5 cut(s)	472, 656, 854, 2059, 2669
GsaI	CCCAGC	3 cut(s)	1035, 1833, 1869
GsuI	CTGGAG	1 cut(s)	2280
HaeII	RGCGCY	1 cut(s)	2061
HaeIII	GGCC	8 cut(s)	38, 168, 332, 440, 444, 509, 1882, 2241
HapII	CCGG	6 cut(s)	221, 441, 453, 615, 885, 2644
HgaI	GACGC	5 cut(s)	281, 337, 920, 1583, 2582
HhaI	GCGC	5 cut(s)	473, 657, 855, 2060, 2670
Hin1I	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
Hin6I	GCGC	5 cut(s)	471, 655, 853, 2058, 2668
HinP1I	GCGC	5 cut(s)	471, 655, 853, 2058, 2668
HincII	GTYRAC	1 cut(s)	2107
HindII	GTYRAC	1 cut(s)	2107
HinfI	GANTC	5 cut(s)	217, 362, 677, 1439, 1726
HpaI	GTTAAC	1 cut(s)	2107
HpaII	CCGG	6 cut(s)	221, 441, 453, 615, 885, 2644
HphI	GGTGA	8 cut(s)	91, 778, 874, 959, 1136, 1201, 1991, 2413
Hpy166II	GTNNAC	4 cut(s)	910, 1843, 2107, 2463
Hpy188III	TCNNGA	9 cut(s)	493, 584, 668, 968, 1281, 1465, 1778, 2113, 2297
Hpy8I	GTNNAC	4 cut(s)	910, 1843, 2107, 2463
Hpy99I	CGWCG	5 cut(s)	275, 320, 323, 326, 536
HpyCH4III	ACNGT	4 cut(s)	574, 642, 2156, 2175
HpyCH4IV	ACGT	5 cut(s)	231, 324, 408, 534, 1421
HpyF3I	CTNAG	7 cut(s)	14, 205, 344, 1560, 2324, 2489, 2613
HpySE526I	ACGT	5 cut(s)	231, 324, 408, 534, 1421
Hsp92I	GRCGYC	6 cut(s)	273, 324, 408, 534, 912, 2574
HspAI	GCGC	5 cut(s)	471, 655, 853, 2058, 2668
KroI	GCCGGC	2 cut(s)	440, 614
KroNI	GCCGGC	2 cut(s)	442, 616
KspAI	GTTAAC	1 cut(s)	2107
LmnI	GCTCC	8 cut(s)	56, 68, 181, 196, 1046, 1862, 2332, 2506
Lsp1109I	GCAGC	8 cut(s)	47, 103, 106, 147, 405, 1207, 1669, 1927
LweI	GCATC	6 cut(s)	73, 721, 1087, 1797, 1902, 1984
MaeI	CTAG	4 cut(s)	23, 1496, 1893, 2453
MaeII	ACGT	5 cut(s)	231, 324, 408, 534, 1421
MaeIII	GTNAC	8 cut(s)	880, 1207, 1483, 1517, 1715, 2156, 2264, 2546
MbiI	CCGCTC	2 cut(s)	191, 1501
MfeI	CAATTG	1 cut(s)	857
MflI	RGATCY	3 cut(s)	495, 1283, 1943
MhlI	GDGCHC	2 cut(s)	1095, 2304
MluI	ACGCGT	1 cut(s)	1575
MlyI	GAGTC	4 cut(s)	211, 371, 1433, 1720
MmeI	TCCRAC	6 cut(s)	661, 1020, 1059, 1782, 2543, 2684
MroNI	GCCGGC	2 cut(s)	440, 614
MroXI	GAANNNNTTC	1 cut(s)	2006
MseI	TTAA	6 cut(s)	1608, 1656, 1794, 2106, 2595, 2686
MslI	CAYNNNNRTG	1 cut(s)	2514
MspA1I	CMGCKG	3 cut(s)	116, 1833, 1918
MspI	CCGG	6 cut(s)	221, 441, 453, 615, 885, 2644
MspR9I	CCNGG	3 cut(s)	453, 674, 886
MunI	CAATTG	1 cut(s)	857
Mva1269I	GAATGC	4 cut(s)	709, 1222, 1384, 2281
MvaI	CCWGG	1 cut(s)	674
MvnI	CGCG	3 cut(s)	471, 1017, 1577
NaeI	GCCGGC	2 cut(s)	442, 616
NciI	CCSGG	2 cut(s)	453, 886
NcoI	CCATGG	1 cut(s)	661
NdeI	CATATG	1 cut(s)	1528
NgoMIV	GCCGGC	2 cut(s)	440, 614
NlaIV	GGNNCC	3 cut(s)	169, 1827, 2142
NmeAIII	GCCGAG	1 cut(s)	1475
NmuCI	GTSAC	3 cut(s)	880, 1207, 1715
NspI	RCATGY	2 cut(s)	893, 2513
PaeI	GCATGC	2 cut(s)	893, 2513
PaeR7I	CTCGAG	2 cut(s)	374, 1463
PcsI	WCGNNNNNNNCGW	5 cut(s)	279, 396, 405, 570, 876
PctI	GAATGC	4 cut(s)	709, 1222, 1384, 2281
PdiI	GCCGGC	2 cut(s)	442, 616
PdmI	GAANNNNTTC	1 cut(s)	2006
PfeI	GAWTC	1 cut(s)	677
PflFI	GACNNNGTC	2 cut(s)	233, 323
PflMI	CCANNNNNTGG	1 cut(s)	667
PleI	GAGTC	4 cut(s)	211, 370, 1433, 1720
PpsI	GAGTC	4 cut(s)	211, 370, 1433, 1720
PpuMI	RGGWCCY	1 cut(s)	379
Psp124BI	GAGCTC	1 cut(s)	2304
Psp1406I	AACGTT	1 cut(s)	1421
Psp5II	RGGWCCY	1 cut(s)	379
Psp6I	CCWGG	1 cut(s)	672
PspEI	GGTNACC	2 cut(s)	1207, 1483
PspFI	CCCAGC	3 cut(s)	1031, 1829, 1865
PspGI	CCWGG	1 cut(s)	672
PspN4I	GGNNCC	3 cut(s)	169, 1827, 2142
PspPI	GGNCC	7 cut(s)	167, 235, 331, 379, 438, 670, 1881
PspPPI	RGGWCCY	1 cut(s)	379
PspXI	VCTCGAGB	1 cut(s)	374
PstNI	CAGNNNCTG	1 cut(s)	2179
PsuI	RGATCY	3 cut(s)	495, 1283, 1943
PsyI	GACNNNGTC	2 cut(s)	233, 323
PvuII	CAGCTG	1 cut(s)	1833
RigI	GGCCGGCC	1 cut(s)	444
RsaI	GTAC	5 cut(s)	563, 1109, 2319, 2583, 2642
RsaNI	GTAC	5 cut(s)	562, 1108, 2318, 2582, 2641
RseI	CAYNNNNRTG	1 cut(s)	2514
SacI	GAGCTC	1 cut(s)	2304
SaqAI	TTAA	6 cut(s)	1608, 1656, 1794, 2106, 2595, 2686
Sau96I	GGNCC	7 cut(s)	167, 235, 331, 379, 438, 670, 1881
SchI	GAGTC	4 cut(s)	211, 371, 1433, 1720
ScrFI	CCNGG	3 cut(s)	453, 674, 886
SduI	GDGCHC	2 cut(s)	1095, 2304
SfaNI	GCATC	6 cut(s)	73, 721, 1087, 1797, 1902, 1984
SfcI	CTRYAG	1 cut(s)	547
Sfr274I	CTCGAG	2 cut(s)	374, 1463
SinI	GGWCC	3 cut(s)	235, 379, 670
SlaI	CTCGAG	2 cut(s)	374, 1463
SmiMI	CAYNNNNRTG	1 cut(s)	2514
SmlI	CTYRAG	6 cut(s)	374, 422, 803, 1148, 1265, 1463
SmoI	CTYRAG	6 cut(s)	374, 422, 803, 1148, 1265, 1463
SphI	GCATGC	2 cut(s)	893, 2513
SspMI	CTAG	4 cut(s)	23, 1496, 1893, 2453
SstI	GAGCTC	1 cut(s)	2304
StyD4I	CCNGG	3 cut(s)	451, 672, 884
StyI	CCWWGG	3 cut(s)	33, 504, 661
TaaI	ACNGT	4 cut(s)	574, 642, 2156, 2175
TaiI	ACGT	5 cut(s)	234, 327, 411, 537, 1424
TatI	WGTACW	1 cut(s)	2581
TauI	GCSGC	4 cut(s)	113, 116, 447, 2244
TfiI	GAWTC	1 cut(s)	677
Tru1I	TTAA	6 cut(s)	1608, 1656, 1794, 2106, 2595, 2686
Tru9I	TTAA	6 cut(s)	1608, 1656, 1794, 2106, 2595, 2686
TscAI	CASTG	1 cut(s)	904
TseFI	GTSAC	3 cut(s)	880, 1207, 1715
TseI	GCWGC	8 cut(s)	60, 116, 119, 135, 393, 1220, 1682, 1915
Tsp45I	GTSAC	3 cut(s)	880, 1207, 1715
TspDTI	ATGAA	7 cut(s)	1193, 1229, 1394, 1487, 1528, 1843, 2138
TspGWI	ACGGA	1 cut(s)	1875
TspRI	CASTG	1 cut(s)	904
Tth111I	GACNNNGTC	2 cut(s)	233, 323
Van91I	CCANNNNNTGG	1 cut(s)	667
VpaK11BI	GGWCC	3 cut(s)	235, 379, 670
XagI	CCTNNNNNAGG	2 cut(s)	503, 1151
XapI	RAATTY	3 cut(s)	1926, 2195, 2419
XceI	RCATGY	2 cut(s)	893, 2513
XhoI	CTCGAG	2 cut(s)	374, 1463
XmiI	GTMKAC	1 cut(s)	1842
XmnI	GAANNNNTTC	1 cut(s)	2006
XspI	CTAG	4 cut(s)	23, 1496, 1893, 2453
ZraI	GACGTC	3 cut(s)	325, 409, 535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.