Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007971

Physical Location & Seq

Genomic Coordinates Reverse (-)

31949691 .. 31955133

Gene Structure 5443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00025450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGCTTTTTTCACTGAAAAATCTAGCCAAATCTTCTTCTCATAGGACCAAGTCACTTGGCATCCTCACCGGCATTGATTCAACTTCAATTCCATTCACCACCACCGCCGCCGCCGCAACAACCACCACTACAACGTGCTTGATCCCGGCCAAGGACATCGTCCTCTCCTTCAAGCAATGGTTCAAGTCCGGCAACCACCTCCTCCTCCACCAAATCTTCCAAATCCTCAAAGCCACCTCCGACAGCGACCACTTCAACCACCACCACCACCACCAGCACTCCTCCGCCGACCTCGCCCTCTCGCATCTCGACCTCCACCTCTCCGAGGCTTTAGTGCTCGAGGTGCTCCGCTACGGCCACGACCACGAGCACGACGTCCTCTCCTGCCTCAAGTTCTTCGACTGGGTCGGCCGCCAGAACAGCTTCTACCACACGCGCGCCACCTTCAACGCCATTTTCAAGATCCTCTCACGCGCCAAGCTCATGTCCCTCATGCTCGACTTCCTCGCCAACTTCCGCAGGCACAAGTTCGACCACGGCGTTCGGTTTCATGATACATTGGTCATGGGCTACGCCGTCTCCGGCAAGCCTGAAGTTGCGTTGCAGCTGTTCGGCAGAATGCGATTTCAGGGACTCGACTTGGATGCTTTTGGCTACCATGTGCTTTTGAATGCTCTGGTGGAGGAGAAGTGTTTCGACGCCGTTGAGGTCGTAGCTAATCAGATTTCCATGAGGGGTTTCGAGAATGAAATCACGCATTCCGTCATGCTCAAGTGGTTCTGCAAGCAGAACTTGTTGGATGAGGCGGAGGGGTATTTGAGAAGATTAGTGACTGAGGGCAAGGCCAATGGCTCTGCGGTGGGGGTTCTTGTTCGTGCATTGTGTCAGCATAAGAAGTTCGAGCGTGCTACTAAGTTGATGGATGAGTTTCGGGACTTAGGGGTGGTTTCTATGGAGAGTGCTTATGCTGTGTGGATTGGAGACCTTGCGCAGGCTGGGAGGTTAGATCAAGCTTTGGAGTTTTTACAGAGCAAGAAGTCATTGGAAGGGTATGTTCCCGATGTCTTTAGGTACAATATTTTGATTGGTAAACTTTTGAGGGAGGTTAGACTCGAGGAGGTTTGTGATCTCATGTTGGAGATGAAGGAGCGTGAGATATCACCCGATGCGGTCACTATGAATGCTGCATTGTGCTTCTTTTGTAAAGTGGGAATGCTGGATATCGCGATTGAGTTGTATAATTCAAGGTCTGAGTTTGGGCTTACCCCCAATAGCATGGCTTACAACTATTTGATCAATACTTTCTGCGGGGATGGCAGCGTTGACGAAGCATATGGAGTATTGAAATCCTCTCTTGACCAAGGTTACTTTCCAGGTAGGAAAACATTTTCTATATTGGCAGATGCTTTGTGCAGAGAGGGAAAGCTTGAGAAGATGAAGGAGTTGGTTATTTTTGCTTTAGAGCGGAATCTTATGCCGAGTGGTTCCACCTATGACAAGTTCATTACTACTCTTTGCAAAACTAGGAGGGTAGAAGACGGTTATTTGATACATGAGGAACTTAACAGAATAAGTGAAGTTCGTAGAGAGAGTACATACATCAACTTGATTGAAGGTTTTTGCAAGTTAAATAAGGGTGATATTGCCGCTAGACTTCTGATTGAAATGCAAGTACATGGTCACTCACCAACTCATAAGCTGTTCCGAGATGTAATTTGCTCTTTATGTGATACGGGAAATCCGGTAAATACAGACCAGCAATTTTTCACCTTATTGGAGATGCAGTTGTCTTGCCGGGAACCTACTTGTCGTATATACAACTTCTTCATCTATGGAGCTGGGCATGCCAAAAGGCCTGATTTGGCTAGAAAAGTATTAGAGATGATGCAGCATAGTGGGATTGAACCTAATTTGAGTTCTGATGTTCTTATGTTGCAGAGTTATTTGAAGAGTGAACGATATTCGGATGCTTTGAACTACTTCAATGATCTGCGTCACAGAAGAAAGATAAGGAGAAGACTTTACAGTGCCATGATTACTGGTATGTGCAAGGCTAAAAAAGCAGATATTGCACTTAACTTACTTAGGGAAATGAGAGAGAACGGAGTGGTTCCAAGCGATGACTGCTATGAGTTTCTTATACAATTGCTCTGCTGGAATAAAGAATACGATATAGCAGTAATCCTCATTAAGGACTTAGAGAAAGTCGGCCGCCACATTACATCCTTTATTGGCAACATACTTCTGTTGCATTCTTTGAAAAGTCAAGAGCTGTACCAGGCTTGGGTTCGTCTAAGAGAGGTGCAAATCGAGATCTCTGATAATTCAATCCTTGGTCTGCTTATTGGTGCATTTTCTGGTCGTATTTGGGTGAGCCAACAGATTGAGAACTTGGAAGAAGTAATTGCTAGCTGCTTTCCGCTAGACGTGTACACATACAATTTATTACTTAGATGTTTCAGTAATAGCAACATGGACCATGCTTGTGCATTGTTCTATAGGATGTCACAGAAAGGGTATGAGCCCAATCAGTGGACTTATGAAATCTTAGTACAAGGTTTTCTTAAGCATGGGAGGACAGACGAGGCAAACAGATGGCTAAAAGCTATGTACGTGAAAGGTTTTAGTCCAAACAAGCAAACGATGCGATTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

885

Amino Acids

101.46

Weight (kDa)

7.37

Isoelectric Point (pI)

38.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	353 - 395	3e-08	PPR repeat family
PPR_2	PF13041	388 - 436	4.8e-10	PPR repeat family
PPR_1	PF12854	420 - 449	5.7e-06	PPR repeat
PPR_2	PF13041	423 - 472	9.2e-08	PPR repeat family
PPR_2	PF13041	675 - 718	1.7e-08	PPR repeat family
PPR_2	PF13041	809 - 856	2.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71210
fragaria_vesca	FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690 FvH4_3g29690
malus_domestica	MD05G1041400.v1.1 MD10G1047800.v1.1
prunus_persica	Prupe.8G057300_v2.0.a1 Prupe.8G057300_v2.0.a1
pyrus_communis	pycom05g03410 pycom10g03380 pycom17g22250
rosa_chinensis	RchiOBHm_Chr5g0055041
rosa_laevigata	RLG00000034959
rosa_multiflora	Rmu_sc0000925.1_g000011
rosa_roxburghii	Rroxscaffold_1G00025450
rosa_rugosa	Rorug05G0290400
rosa_samantha	Rh5AG360300 Rh5BG371300 Rh5CG393600 Rh5DG385100
rosa_wichuraiana	Rw5G033900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	378
Acc16I	TGCGCA	1 cut(s)	990
AccB7I	CCANNNNNTGG	1 cut(s)	2532
AccBSI	CCGCTC	1 cut(s)	1465
AccII	CGCG	4 cut(s)	436, 438, 474, 1226
AclWI	GGATC	2 cut(s)	136, 457
AcoI	YGGCCR	4 cut(s)	147, 355, 409, 2209
AcuI	CTGAAG	1 cut(s)	612
AcyI	GRCGYC	2 cut(s)	375, 699
AfaI	GTAC	7 cut(s)	1073, 1594, 1674, 2276, 2432, 2553, 2612
AfiI	CCNNNNNNNGG	6 cut(s)	151, 325, 991, 2191, 2284, 2532
AflII	CTTAAG	1 cut(s)	2564
AflIII	ACRYGT	1 cut(s)	2427
AjiI	CACGTC	1 cut(s)	2428
AjnI	CCWGG	2 cut(s)	1372, 2277
AjuI	GAANNNNNNNTTGG	2 cut(s)	1353, 1385
Alw21I	GWGCWC	3 cut(s)	339, 348, 372
Alw26I	GTCTC	2 cut(s)	583, 975
AlwI	GGATC	2 cut(s)	136, 457
Ama87I	CYCGRG	2 cut(s)	338, 1112
AoxI	GGCC	6 cut(s)	147, 355, 409, 843, 1853, 2209
ApeKI	GCWGC	5 cut(s)	604, 1184, 1317, 1888, 2412
Asp700I	GAANNNNTTC	3 cut(s)	422, 692, 1979
AspLEI	GCGC	4 cut(s)	438, 440, 476, 991
AspS9I	GGNCC	2 cut(s)	45, 2476
AsuC2I	CCSGG	2 cut(s)	146, 1796
AsuHPI	GGTGA	7 cut(s)	58, 88, 1152, 1649, 1677, 1759, 2383
AsuNHI	GCTAGC	1 cut(s)	2408
AvaI	CYCGRG	2 cut(s)	338, 1112
AvaII	GGWCC	2 cut(s)	45, 2476
BaeI	ACNNNNGTAYC	2 cut(s)	546, 579
BanII	GRGCYC	1 cut(s)	2526
BauI	CACGAG	1 cut(s)	365
BbsI	GAAGAC	2 cut(s)	1542, 2023
Bbv12I	GWGCWC	3 cut(s)	339, 348, 372
BbvI	GCAGC	5 cut(s)	616, 1171, 1329, 1900, 2399
BccI	CCATC	3 cut(s)	913, 1307, 2589
BceAI	ACGGC	4 cut(s)	370, 553, 560, 686
BcgI	CGANNNNNNTGC	2 cut(s)	626, 660
BciT130I	CCWGG	2 cut(s)	1374, 2279
BclI	TGATCA	1 cut(s)	1293
BcnI	CCSGG	2 cut(s)	146, 1796
BcoDI	GTCTC	2 cut(s)	583, 975
BfaI	CTAG	6 cut(s)	23, 1524, 1650, 1866, 2409, 2423
BfmI	CTRYAG	1 cut(s)	2497
BfrI	CTTAAG	1 cut(s)	2564
BglII	AGATCT	1 cut(s)	2313
Bme1390I	CCNGG	4 cut(s)	146, 1374, 1796, 2279
Bme18I	GGWCC	2 cut(s)	45, 2476
BmeT110I	CYCGRG	2 cut(s)	338, 1112
BmgBI	CACGTC	1 cut(s)	2428
BmgT120I	GGNCC	2 cut(s)	45, 2476
BmiI	GGNNCC	3 cut(s)	1486, 1800, 2112
BmrFI	CCNGG	4 cut(s)	146, 1374, 1796, 2279
BmrI	ACTGGG	1 cut(s)	412
BmsI	GCATC	9 cut(s)	69, 313, 634, 1156, 1393, 1770, 1875, 1957, 2634
BmtI	GCTAGC	1 cut(s)	2412
BmuI	ACTGGG	1 cut(s)	412
BpiI	GAAGAC	2 cut(s)	1542, 2023
BpuEI	CTTGAG	3 cut(s)	374, 755, 1448
BpuMI	CCSGG	2 cut(s)	146, 1796
BsaAI	YACGTR	1 cut(s)	2614
BsaHI	GRCGYC	2 cut(s)	375, 699
BsaI	GGTCTC	1 cut(s)	975
BsaJI	CCNNGG	5 cut(s)	150, 324, 535, 1360, 2332
BsaWI	WCCGGW	1 cut(s)	1741
Bsc4I	CCNNNNNNNGG	6 cut(s)	151, 325, 991, 2191, 2284, 2532
Bse118I	RCCGGY	1 cut(s)	68
Bse1I	ACTGG	2 cut(s)	407, 2044
Bse3DI	GCAATG	1 cut(s)	182
BseBI	CCWGG	2 cut(s)	1374, 2279
BseDI	CCNNGG	5 cut(s)	150, 324, 535, 1360, 2332
BseGI	GGATG	8 cut(s)	60, 649, 805, 928, 1318, 1972, 2222, 2508
BseLI	CCNNNNNNNGG	6 cut(s)	151, 325, 991, 2191, 2284, 2532
BseMI	GCAATG	1 cut(s)	182
BseMII	CTCAG	2 cut(s)	825, 1242
BseNI	ACTGG	2 cut(s)	407, 2044
BsePI	GCGCGC	1 cut(s)	436
BseRI	GAGGAG	5 cut(s)	191, 194, 271, 698, 1130
BseX3I	CGGCCG	2 cut(s)	409, 2209
BseXI	GCAGC	5 cut(s)	616, 1171, 1329, 1900, 2399
BseYI	CCCAGC	2 cut(s)	995, 1838
BsgI	GTGCAG	1 cut(s)	1432
Bsh1236I	CGCG	4 cut(s)	436, 438, 474, 1226
Bsh1285I	CGRYCG	2 cut(s)	412, 2212
BshFI	GGCC	6 cut(s)	149, 357, 411, 845, 1855, 2211
BsiEI	CGRYCG	2 cut(s)	412, 2212
BsiHKAI	GWGCWC	3 cut(s)	339, 348, 372
BsiHKCI	CYCGRG	2 cut(s)	338, 1112
BsiSI	CCGG	6 cut(s)	69, 146, 189, 582, 1742, 1795
BslFI	GGGAC	3 cut(s)	472, 645, 947
BslI	CCNNNNNNNGG	6 cut(s)	151, 325, 991, 2191, 2284, 2532
BsmAI	GTCTC	2 cut(s)	583, 975
BsmBI	CGTCTC	1 cut(s)	583
BsmFI	GGGAC	3 cut(s)	472, 645, 947
BsmI	GAATGC	6 cut(s)	624, 676, 757, 1186, 1218, 2251
BsnI	GGCC	6 cut(s)	149, 357, 411, 845, 1855, 2211
Bso31I	GGTCTC	1 cut(s)	975
BsoBI	CYCGRG	2 cut(s)	338, 1112
Bsp1286I	GDGCHC	4 cut(s)	339, 348, 372, 2526
Bsp1407I	TGTACA	1 cut(s)	2430
Bsp143I	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
Bsp68I	TCGCGA	1 cut(s)	1226
BspANI	GGCC	6 cut(s)	149, 357, 411, 845, 1855, 2211
BspCNI	CTCAG	2 cut(s)	826, 1243
BspFNI	CGCG	4 cut(s)	436, 438, 474, 1226
BspHI	TCATGA	1 cut(s)	550
BspLI	GGNNCC	3 cut(s)	1486, 1800, 2112
BspOI	GCTAGC	1 cut(s)	2412
BspPI	GGATC	2 cut(s)	136, 457
BspTI	CTTAAG	1 cut(s)	2564
BspTNI	GGTCTC	1 cut(s)	975
BsrBI	CCGCTC	1 cut(s)	1465
BsrDI	GCAATG	1 cut(s)	182
BsrFI	RCCGGY	1 cut(s)	68
BsrGI	TGTACA	1 cut(s)	2430
BsrI	ACTGG	2 cut(s)	407, 2044
BssAI	RCCGGY	1 cut(s)	68
BssECI	CCNNGG	5 cut(s)	150, 324, 535, 1360, 2332
BssHII	GCGCGC	1 cut(s)	436
BssMI	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
BssNI	GRCGYC	2 cut(s)	375, 699
BssSI	CACGAG	1 cut(s)	365
BssT1I	CCWWGG	3 cut(s)	150, 1360, 2332
Bst2BI	CACGAG	1 cut(s)	365
Bst2UI	CCWGG	2 cut(s)	1374, 2279
Bst4CI	ACNGT	2 cut(s)	1541, 2027
Bst6I	CTCTTC	1 cut(s)	1943
BstACI	GRCGYC	2 cut(s)	375, 699
BstAFI	CTTAAG	1 cut(s)	2564
BstAPI	GCANNNNNTGC	2 cut(s)	2069, 2644
BstAUI	TGTACA	1 cut(s)	2430
BstBAI	YACGTR	1 cut(s)	2614
BstC8I	GCNNGC	8 cut(s)	438, 521, 587, 785, 906, 993, 1845, 2410
BstDEI	CTNAG	9 cut(s)	834, 912, 937, 1251, 2084, 2197, 2294, 2450, 2548
BstDSI	CCRYGG	1 cut(s)	535
BstENI	CCTNNNNNAGG	3 cut(s)	323, 989, 2189
BstF5I	GGATG	8 cut(s)	60, 649, 805, 928, 1318, 1972, 2222, 2508
BstFNI	CGCG	4 cut(s)	436, 438, 474, 1226
BstHHI	GCGC	4 cut(s)	438, 440, 476, 991
BstKTI	GATC	7 cut(s)	144, 465, 1009, 1129, 1296, 1990, 2316
BstMAI	GTCTC	2 cut(s)	583, 975
BstMBI	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
BstMCI	CGRYCG	2 cut(s)	412, 2212
BstNI	CCWGG	2 cut(s)	1374, 2279
BstNSI	RCATGY	1 cut(s)	1847
BstSCI	CCNGG	4 cut(s)	144, 1372, 1794, 2277
BstSFI	CTRYAG	1 cut(s)	2497
BstUI	CGCG	4 cut(s)	436, 438, 474, 1226
BstV1I	GCAGC	5 cut(s)	616, 1171, 1329, 1900, 2399
BstV2I	GAAGAC	2 cut(s)	1542, 2023
BstX2I	RGATCY	2 cut(s)	462, 2313
BstXI	CCANNNNNNTGG	1 cut(s)	1276
BstYI	RGATCY	2 cut(s)	462, 2313
BstZI	CGGCCG	2 cut(s)	409, 2209
BsuRI	GGCC	6 cut(s)	149, 357, 411, 845, 1855, 2211
BtgI	CCRYGG	1 cut(s)	535
BtgZI	GCGATG	1 cut(s)	2133
BtrI	CACGTC	1 cut(s)	2428
BtsCI	GGATG	8 cut(s)	60, 649, 805, 928, 1318, 1972, 2222, 2508
BtsIMutI	CAGTG	3 cut(s)	11, 2032, 2537
BtuMI	TCGCGA	1 cut(s)	1226
Cac8I	GCNNGC	8 cut(s)	438, 521, 587, 785, 906, 993, 1845, 2410
CciI	TCATGA	1 cut(s)	550
CfoI	GCGC	4 cut(s)	438, 440, 476, 991
Cfr10I	RCCGGY	1 cut(s)	68
Cfr13I	GGNCC	2 cut(s)	45, 2476
CseI	GACGC	2 cut(s)	707, 1982
Csp6I	GTAC	7 cut(s)	1072, 1593, 1673, 2275, 2431, 2552, 2611
CviQI	GTAC	7 cut(s)	1072, 1593, 1673, 2275, 2431, 2552, 2611
DdeI	CTNAG	9 cut(s)	834, 912, 937, 1251, 2084, 2197, 2294, 2450, 2548
DpnI	GATC	7 cut(s)	143, 464, 1008, 1128, 1295, 1989, 2315
DpnII	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
EaeI	YGGCCR	4 cut(s)	147, 355, 409, 2209
EagI	CGGCCG	2 cut(s)	409, 2209
Eam1104I	CTCTTC	1 cut(s)	1943
EarI	CTCTTC	1 cut(s)	1943
EciI	GGCGGA	2 cut(s)	274, 821
EclXI	CGGCCG	2 cut(s)	409, 2209
Eco130I	CCWWGG	3 cut(s)	150, 1360, 2332
Eco147I	AGGCCT	1 cut(s)	1855
Eco24I	GRGCYC	1 cut(s)	2526
Eco31I	GGTCTC	1 cut(s)	975
Eco32I	GATATC	2 cut(s)	1158, 1222
Eco47I	GGWCC	2 cut(s)	45, 2476
Eco52I	CGGCCG	2 cut(s)	409, 2209
Eco57I	CTGAAG	1 cut(s)	612
Eco88I	CYCGRG	2 cut(s)	338, 1112
EcoNI	CCTNNNNNAGG	3 cut(s)	323, 989, 2189
EcoRII	CCWGG	2 cut(s)	1372, 2277
EcoRV	GATATC	2 cut(s)	1158, 1222
EcoT14I	CCWWGG	3 cut(s)	150, 1360, 2332
EcoT38I	GRGCYC	1 cut(s)	2526
ErhI	CCWWGG	3 cut(s)	150, 1360, 2332
Esp3I	CGTCTC	1 cut(s)	583
FalI	AAGNNNNNCTT	4 cut(s)	776, 808, 2547, 2579
FaqI	GGGAC	3 cut(s)	472, 645, 947
FauI	CCCGC	1 cut(s)	1301
FauNDI	CATATG	1 cut(s)	1333
FbaI	TGATCA	1 cut(s)	1293
FokI	GGATG	8 cut(s)	47, 656, 812, 935, 1325, 1979, 2209, 2515
FriOI	GRGCYC	1 cut(s)	2526
FspBI	CTAG	6 cut(s)	23, 1524, 1650, 1866, 2409, 2423
FspI	TGCGCA	1 cut(s)	990
GlaI	GCGC	4 cut(s)	437, 439, 475, 990
GsaI	CCCAGC	2 cut(s)	999, 1842
HaeIII	GGCC	6 cut(s)	149, 357, 411, 845, 1855, 2211
HapII	CCGG	6 cut(s)	69, 146, 189, 582, 1742, 1795
HgaI	GACGC	2 cut(s)	707, 1982
HhaI	GCGC	4 cut(s)	438, 440, 476, 991
Hin1I	GRCGYC	2 cut(s)	375, 699
Hin6I	GCGC	4 cut(s)	436, 438, 474, 989
HinP1I	GCGC	4 cut(s)	436, 438, 474, 989
HincII	GTYRAC	1 cut(s)	1324
HindII	GTYRAC	1 cut(s)	1324
HindIII	AAGCTT	2 cut(s)	1011, 1424
HinfI	GANTC	4 cut(s)	77, 633, 1110, 1468
HpaII	CCGG	6 cut(s)	69, 146, 189, 582, 1742, 1795
HphI	GGTGA	7 cut(s)	58, 88, 1152, 1649, 1677, 1759, 2383
Hpy166II	GTNNAC	6 cut(s)	1091, 1324, 1955, 2431, 2433, 2535
Hpy188I	TCNGA	9 cut(s)	241, 325, 723, 1252, 1659, 1706, 1921, 1966, 2320
Hpy8I	GTNNAC	6 cut(s)	1091, 1324, 1955, 2431, 2433, 2535
Hpy99I	CGWCG	2 cut(s)	377, 701
HpyAV	CCTTC	6 cut(s)	178, 454, 1040, 1138, 1432, 1607
HpyCH4III	ACNGT	2 cut(s)	1541, 2027
HpyCH4IV	ACGT	4 cut(s)	134, 375, 2427, 2613
HpyF3I	CTNAG	9 cut(s)	834, 912, 937, 1251, 2084, 2197, 2294, 2450, 2548
HpySE526I	ACGT	4 cut(s)	134, 375, 2427, 2613
Hsp92I	GRCGYC	2 cut(s)	375, 699
HspAI	GCGC	4 cut(s)	436, 438, 474, 989
Ksp22I	TGATCA	1 cut(s)	1293
Kzo9I	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
LmnI	GCTCC	3 cut(s)	351, 1147, 1835
Lsp1109I	GCAGC	5 cut(s)	616, 1171, 1329, 1900, 2399
LweI	GCATC	9 cut(s)	69, 313, 634, 1156, 1393, 1770, 1875, 1957, 2634
MaeI	CTAG	6 cut(s)	23, 1524, 1650, 1866, 2409, 2423
MaeII	ACGT	4 cut(s)	134, 375, 2427, 2613
MaeIII	GTNAC	7 cut(s)	51, 829, 1171, 1364, 1679, 1994, 2505
MalI	GATC	7 cut(s)	143, 464, 1008, 1128, 1295, 1989, 2315
MbiI	CCGCTC	1 cut(s)	1465
MboI	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
MfeI	CAATTG	1 cut(s)	2144
MflI	RGATCY	2 cut(s)	462, 2313
MhlI	GDGCHC	4 cut(s)	339, 348, 372, 2526
MluCI	AATT	9 cut(s)	87, 1240, 1713, 1760, 1909, 2144, 2323, 2403, 2440
MlyI	GAGTC	2 cut(s)	627, 1104
MmeI	TCCRAC	3 cut(s)	264, 777, 1116
MroXI	GAANNNNTTC	3 cut(s)	422, 692, 1979
MseI	TTAA	6 cut(s)	1563, 1628, 2076, 2190, 2565, 2656
MslI	CAYNNNNRTG	1 cut(s)	2484
MspA1I	CMGCKG	1 cut(s)	607
MspCI	CTTAAG	1 cut(s)	2564
MspI	CCGG	6 cut(s)	69, 146, 189, 582, 1742, 1795
MspR9I	CCNGG	4 cut(s)	146, 1374, 1796, 2279
MunI	CAATTG	1 cut(s)	2144
Mva1269I	GAATGC	6 cut(s)	624, 676, 757, 1186, 1218, 2251
MvaI	CCWGG	2 cut(s)	1374, 2279
MvnI	CGCG	4 cut(s)	436, 438, 474, 1226
NciI	CCSGG	2 cut(s)	146, 1796
NdeI	CATATG	1 cut(s)	1333
NdeII	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
NheI	GCTAGC	1 cut(s)	2408
NlaIV	GGNNCC	3 cut(s)	1486, 1800, 2112
NmeAIII	GCCGAG	1 cut(s)	1503
NmuCI	GTSAC	6 cut(s)	51, 829, 1171, 1679, 1994, 2505
NruI	TCGCGA	1 cut(s)	1226
NsbI	TGCGCA	1 cut(s)	990
NspI	RCATGY	1 cut(s)	1847
PaeI	GCATGC	1 cut(s)	1847
PaeR7I	CTCGAG	2 cut(s)	338, 1112
PagI	TCATGA	1 cut(s)	550
PauI	GCGCGC	1 cut(s)	436
PceI	AGGCCT	1 cut(s)	1855
PcsI	WCGNNNNNNNCGW	2 cut(s)	372, 537
PctI	GAATGC	6 cut(s)	624, 676, 757, 1186, 1218, 2251
PdmI	GAANNNNTTC	3 cut(s)	422, 692, 1979
PfeI	GAWTC	2 cut(s)	77, 1468
PflFI	GACNNNGTC	3 cut(s)	49, 158, 404
PflMI	CCANNNNNTGG	1 cut(s)	2532
PleI	GAGTC	2 cut(s)	627, 1104
PpsI	GAGTC	2 cut(s)	627, 1104
Ppu21I	YACGTR	1 cut(s)	2614
Psp6I	CCWGG	2 cut(s)	1372, 2277
PspFI	CCCAGC	2 cut(s)	995, 1838
PspGI	CCWGG	2 cut(s)	1372, 2277
PspN4I	GGNNCC	3 cut(s)	1486, 1800, 2112
PspPI	GGNCC	2 cut(s)	45, 2476
PspXI	VCTCGAGB	2 cut(s)	338, 1112
PsrI	GAACNNNNNNTAC	2 cut(s)	410, 442
PsuI	RGATCY	2 cut(s)	462, 2313
PsyI	GACNNNGTC	3 cut(s)	49, 158, 404
PteI	GCGCGC	1 cut(s)	436
PvuII	CAGCTG	1 cut(s)	607
RruI	TCGCGA	1 cut(s)	1226
RsaI	GTAC	7 cut(s)	1073, 1594, 1674, 2276, 2432, 2553, 2612
RsaNI	GTAC	7 cut(s)	1072, 1593, 1673, 2275, 2431, 2552, 2611
RseI	CAYNNNNRTG	1 cut(s)	2484
SaqAI	TTAA	6 cut(s)	1563, 1628, 2076, 2190, 2565, 2656
Sau3AI	GATC	7 cut(s)	141, 462, 1006, 1126, 1293, 1987, 2313
Sau96I	GGNCC	2 cut(s)	45, 2476
SchI	GAGTC	2 cut(s)	627, 1104
ScrFI	CCNGG	4 cut(s)	146, 1374, 1796, 2279
SduI	GDGCHC	4 cut(s)	339, 348, 372, 2526
SfaNI	GCATC	9 cut(s)	69, 313, 634, 1156, 1393, 1770, 1875, 1957, 2634
SfcI	CTRYAG	1 cut(s)	2497
Sfr274I	CTCGAG	2 cut(s)	338, 1112
SinI	GGWCC	2 cut(s)	45, 2476
SlaI	CTCGAG	2 cut(s)	338, 1112
SmiMI	CAYNNNNRTG	1 cut(s)	2484
SmlI	CTYRAG	6 cut(s)	338, 389, 770, 1112, 1427, 2564
SmoI	CTYRAG	6 cut(s)	338, 389, 770, 1112, 1427, 2564
SphI	GCATGC	1 cut(s)	1847
Sse9I	AATT	9 cut(s)	87, 1240, 1713, 1760, 1909, 2144, 2323, 2403, 2440
SseBI	AGGCCT	1 cut(s)	1855
SspI	AATATT	1 cut(s)	1078
SspMI	CTAG	6 cut(s)	23, 1524, 1650, 1866, 2409, 2423
StuI	AGGCCT	1 cut(s)	1855
StyD4I	CCNGG	4 cut(s)	144, 1372, 1794, 2277
StyI	CCWWGG	3 cut(s)	150, 1360, 2332
TaaI	ACNGT	2 cut(s)	1541, 2027
TaiI	ACGT	4 cut(s)	137, 378, 2430, 2616
TasI	AATT	9 cut(s)	87, 1240, 1713, 1760, 1909, 2144, 2323, 2403, 2440
TatI	WGTACW	4 cut(s)	1592, 1672, 2430, 2551
TauI	GCSGC	6 cut(s)	110, 113, 116, 414, 1649, 2214
TfiI	GAWTC	2 cut(s)	77, 1468
Tru1I	TTAA	6 cut(s)	1563, 1628, 2076, 2190, 2565, 2656
Tru9I	TTAA	6 cut(s)	1563, 1628, 2076, 2190, 2565, 2656
TscAI	CASTG	3 cut(s)	18, 2032, 2537
TseFI	GTSAC	6 cut(s)	51, 829, 1171, 1679, 1994, 2505
TseI	GCWGC	5 cut(s)	604, 1184, 1317, 1888, 2412
Tsp45I	GTSAC	6 cut(s)	51, 829, 1171, 1679, 1994, 2505
TspDTI	ATGAA	8 cut(s)	539, 762, 1157, 1193, 1451, 1492, 1816, 2556
TspGWI	ACGGA	2 cut(s)	751, 2118
TspRI	CASTG	3 cut(s)	18, 2032, 2537
Tth111I	GACNNNGTC	3 cut(s)	49, 158, 404
Van91I	CCANNNNNTGG	1 cut(s)	2532
Vha464I	CTTAAG	1 cut(s)	2564
VpaK11BI	GGWCC	2 cut(s)	45, 2476
XagI	CCTNNNNNAGG	3 cut(s)	323, 989, 2189
XceI	RCATGY	1 cut(s)	1847
XhoI	CTCGAG	2 cut(s)	338, 1112
XmnI	GAANNNNTTC	3 cut(s)	422, 692, 1979
XspI	CTAG	6 cut(s)	23, 1524, 1650, 1866, 2409, 2423
ZraI	GACGTC	1 cut(s)	376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.