MD05G1061300.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003742
Physical Location & Seq
Reverse (-)
10751630 .. 10756706
5077 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1061300.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGGGTTCTGCTTGTTGTGTTGCTGCTAGAGACAAGACTATAACAAACACTGCTAGTAGCGAAATTGTACATCGTAATATTCGGTACTCACCAACATGGAGCTTTCGGTGGGATAACCGAGTGGGGGTAGCAGCTGAAGAGACCTCTGTAAGTTGGTTTTCTGATGGTGTTAGCAGAAATGATGGATCGGAAGTTAAATTTGAGTCGGCATGTGTATCTGAGGAGGGAAGTCCATTAGAACATTTTCGAAGACATACACAGCGAAAATCCTCAATTTCTGAGGGAACTGCCGAGCATGTCAGGACTCCTACTTCAGATCGATCCATTTCAAGAAATATTTCCATGGATGCAACTCTTGAACAGACAAAAGAACCAGCAGAGTCCCCTACCGTTTCATATCCATCCCCGACAAAATTATCAGTTTTGCAGCCTTCTACTTCATCCTTGCCCGCATCCCCTTTGTCATCCCAGAGTCACCTGCCTCTTGCTAGCTCAACGCCAGTGAGGTGGCGCCCCTGTTCTCCAGGACATCAGCTATTAAGGCAAGTATCTGATGCCCGAGTCCCAAGAATCAAGTCACCAAACAGCTTCTCAATTTCTGAGGAAAAGGCAAAGCTTCCCTCATGGAGCAATGAATCTGCTAGGGGCTCTCGTGGTGGGTCTTCTGATAGTTGGTCTATGAATGCATTTTCCGAGCTCATGGCCACATCTAATAGAGAAAGGTGGTCTTTTGATAGTGAGTCCTTTGGCTTTAATTGTGAGAAGATAACTAGACCCAGTAGCAGAGTTTCAGCTTCACCCTCTGTTGATTTGCAGACATGTGGAGTTTGCTCAAAGCTTTTGGCTGAGAAATGTTCATGGAGCAGCCAAAAGATTATTGCAACCAATGAGCTTTCTGTGGTTTCAGTGCTAGTTTGTGGACATGTTTATCATGCCGAGTGTTTGGAGAATCTGACACCTGAAATTAACAAGTATGACCCAGCTTGTCCAGTGTGTACTTTTGGGGAGAAACAGATCCACAAGTTGTCTGAAAAAGCATTGAAAGCTGAAATGGATTTGAAGTCTAGAAACAAGAAATCGAGGAACCGGGTGTTAGATCTTGATGGCGATTCTGCTGTGTTTGATCACTTGAAAAGCACCGGAAATCAAGGAAGGGGTCCCAAGTTCGGATCCAGTTCCAGCATGAGAAGCACCTTGGGAAAGCCATTTTTGAGACGGCACTTCTCATTTGGCTCCAAGAGTGCTTCGCTATCAGATAATCATTCTACTAGAAAGAAAGGGTTCTTCTGGGCAAAATCCAGCAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

437

Amino Acids

47.98

Weight (kDa)

9.28

Isoelectric Point (pI)

64.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_Vps41 PF23555 284 - 317 8.4e-06 Vps41 C-terminal RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 510
AciI CCGC 1 cut(s) 450
AclWI GGATC 5 cut(s) 193, 315, 1009, 1164, 1177
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 2 cut(s) 156, 297
AcyI GRCGYC 1 cut(s) 511
AfaI GTAC 3 cut(s) 69, 86, 997
AfiI CCNNNNNNNGG 2 cut(s) 124, 1166
AflIII ACRYGT 2 cut(s) 818, 922
AgsI TTSAA 5 cut(s) 330, 359, 1042, 1060, 1132
AjnI CCWGG 1 cut(s) 523
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 3 cut(s) 24, 134, 1207
AlwI GGATC 5 cut(s) 193, 315, 1009, 1164, 1177
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 4 cut(s) 23, 131, 427, 864
ApoI RAATTY 1 cut(s) 197
Asp700I GAANNNNTTC 3 cut(s) 243, 337, 1280
AspLEI GCGC 1 cut(s) 513
AspS9I GGNCC 1 cut(s) 1157
AsuC2I CCSGG 1 cut(s) 1088
AsuHPI GGTGA 4 cut(s) 81, 467, 570, 789
AsuII TTCGAA 1 cut(s) 247
AsuNHI GCTAGC 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 1 cut(s) 1157
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 1 cut(s) 1169
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 2 cut(s) 650, 699
BauI CACGAG 1 cut(s) 651
BbsI GAAGAC 2 cut(s) 256, 654
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 4 cut(s) 10, 143, 439, 876
BccI CCATC 4 cut(s) 158, 176, 409, 1097
BceAI ACGGC 1 cut(s) 1232
BciT130I CCWGG 1 cut(s) 525
BclI TGATCA 1 cut(s) 1123
BcnI CCSGG 1 cut(s) 1088
BcoDI GTCTC 3 cut(s) 24, 134, 1207
BfaI CTAG 8 cut(s) 27, 54, 489, 642, 771, 911, 1065, 1269
BfoI RGCGCY 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 486
BglII AGATCT 1 cut(s) 1096
BisI GCNGC 4 cut(s) 24, 132, 428, 865
BlsI GCNGC 4 cut(s) 25, 133, 429, 866
Bme1390I CCNGG 2 cut(s) 525, 1088
Bme18I GGWCC 1 cut(s) 1157
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 1157
BmiI GGNNCC 6 cut(s) 512, 1085, 1158, 1159, 1171, 1234
BmrFI CCNGG 2 cut(s) 525, 1088
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 3 cut(s) 337, 461, 544
BmtI GCTAGC 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 2 cut(s) 256, 654
BpmI CTGGAG 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 1088
Bsa29I ATCGAT 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 342, 1194
BsaWI WCCGGW 1 cut(s) 1139
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 1166
Bse1I ACTGG 4 cut(s) 500, 777, 989, 1173
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 525
BseCI ATCGAT 1 cut(s) 319
BseDI CCNNGG 2 cut(s) 342, 1194
BseGI GGATG 5 cut(s) 352, 401, 440, 452, 464
BseLI CCNNNNNNNGG 2 cut(s) 124, 1166
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 4 cut(s) 210, 270, 591, 837
BseNI ACTGG 4 cut(s) 500, 777, 989, 1173
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 4 cut(s) 10, 143, 439, 876
BseYI CCCAGC 1 cut(s) 979
BshFI GGCC 1 cut(s) 704
BshNI GGYRCC 1 cut(s) 510
BshVI ATCGAT 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 2 cut(s) 1087, 1140
BslFI GGGAC 3 cut(s) 367, 548, 1143
BslI CCNNNNNNNGG 2 cut(s) 124, 1166
BsmAI GTCTC 3 cut(s) 24, 134, 1207
BsmBI CGTCTC 1 cut(s) 1207
BsmFI GGGAC 3 cut(s) 367, 548, 1143
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 1 cut(s) 704
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 558
Bsp119I TTCGAA 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 650, 699
Bsp1407I TGTACA 1 cut(s) 67
Bsp143I GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 1 cut(s) 450
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 4 cut(s) 211, 271, 592, 838
BspDI ATCGAT 1 cut(s) 319
BspLI GGNNCC 6 cut(s) 512, 1085, 1158, 1159, 1171, 1234
BspMI ACCTGC 1 cut(s) 486
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 5 cut(s) 193, 315, 1009, 1164, 1177
BspT104I TTCGAA 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 67
BsrI ACTGG 4 cut(s) 500, 777, 989, 1173
BssECI CCNNGG 2 cut(s) 342, 1194
BssMI GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
BssNI GRCGYC 1 cut(s) 511
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 2 cut(s) 342, 1194
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 511
BstAUI TGTACA 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 450, 490
BstDEI CTNAG 4 cut(s) 219, 279, 600, 846
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 5 cut(s) 352, 401, 440, 452, 464
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 7 cut(s) 188, 319, 323, 1017, 1099, 1126, 1172
BstMAI GTCTC 3 cut(s) 24, 134, 1207
BstMBI GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
BstMWI GCNNNNNNNGC 3 cut(s) 541, 1043, 1188
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 4 cut(s) 213, 299, 822, 926
BstSCI CCNGG 2 cut(s) 523, 1086
BstV1I GCAGC 4 cut(s) 10, 143, 439, 876
BstV2I GAAGAC 2 cut(s) 256, 654
BstX2I RGATCY 3 cut(s) 1014, 1096, 1169
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 3 cut(s) 1014, 1096, 1169
Bsu15I ATCGAT 1 cut(s) 319
BsuRI GGCC 1 cut(s) 704
BsuTUI ATCGAT 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 5 cut(s) 352, 401, 440, 452, 464
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 4 cut(s) 48, 507, 912, 996
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 2 cut(s) 450, 490
CfoI GCGC 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 1157
ClaI ATCGAT 1 cut(s) 319
Csp6I GTAC 3 cut(s) 68, 85, 996
CviQI GTAC 3 cut(s) 68, 85, 996
DdeI CTNAG 4 cut(s) 219, 279, 600, 846
DinI GGCGCC 1 cut(s) 512
DpnI GATC 7 cut(s) 187, 318, 322, 1016, 1098, 1125, 1171
DpnII GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 2 cut(s) 342, 1194
Eco24I GRGCYC 2 cut(s) 650, 699
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 1157
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 156, 297
Eco88I CYCGRG 1 cut(s) 558
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 1157
EcoRII CCWGG 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 342, 1194
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 2 cut(s) 650, 699
EgeI GGCGCC 1 cut(s) 512
EheI GGCGCC 1 cut(s) 512
ErhI CCWWGG 2 cut(s) 342, 1194
Esp3I CGTCTC 1 cut(s) 1207
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 3 cut(s) 367, 548, 1143
FauI CCCGC 1 cut(s) 457
FbaI TGATCA 1 cut(s) 1123
Fnu4HI GCNGC 4 cut(s) 24, 132, 428, 865
FokI GGATG 5 cut(s) 359, 388, 427, 439, 451
FriOI GRGCYC 2 cut(s) 650, 699
Fsp4HI GCNGC 4 cut(s) 24, 132, 428, 865
FspBI CTAG 8 cut(s) 27, 54, 489, 642, 771, 911, 1065, 1269
GlaI GCGC 1 cut(s) 512
GluI GCNGC 4 cut(s) 24, 132, 428, 865
GsaI CCCAGC 1 cut(s) 983
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 1 cut(s) 704
HapII CCGG 2 cut(s) 1087, 1140
HhaI GCGC 1 cut(s) 513
Hin1I GRCGYC 1 cut(s) 511
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 614, 836
HpaII CCGG 2 cut(s) 1087, 1140
HphI GGTGA 4 cut(s) 81, 467, 570, 789
Hpy166II GTNNAC 2 cut(s) 920, 996
Hpy188III TCNNGA 5 cut(s) 301, 330, 356, 1065, 1100
Hpy8I GTNNAC 2 cut(s) 920, 996
HpyAV CCTTC 2 cut(s) 441, 1146
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 5 cut(s) 350, 427, 686, 814, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 541, 1043, 1188
HpyF3I CTNAG 4 cut(s) 219, 279, 600, 846
Hsp92I GRCGYC 1 cut(s) 511
HspAI GCGC 1 cut(s) 511
KasI GGCGCC 1 cut(s) 510
KflI GGGWCCC 1 cut(s) 1157
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
LmnI GCTCC 4 cut(s) 99, 627, 861, 1238
Lsp1109I GCAGC 4 cut(s) 10, 143, 439, 876
LweI GCATC 3 cut(s) 337, 461, 544
MaeI CTAG 8 cut(s) 27, 54, 489, 642, 771, 911, 1065, 1269
MaeIII GTNAC 2 cut(s) 473, 576
MalI GATC 7 cut(s) 187, 318, 322, 1016, 1098, 1125, 1171
MboI GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
MboII GAAGA 5 cut(s) 149, 261, 654, 775, 1276
MflI RGATCY 3 cut(s) 1014, 1096, 1169
MhlI GDGCHC 2 cut(s) 650, 699
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 7 cut(s) 63, 197, 273, 413, 594, 754, 963
MluNI TGGCCA 1 cut(s) 704
Mly113I GGCGCC 1 cut(s) 511
MlyI GAGTC 6 cut(s) 212, 298, 389, 481, 570, 749
Mox20I TGGCCA 1 cut(s) 704
Mph1103I ATGCAT 1 cut(s) 688
MroXI GAANNNNTTC 3 cut(s) 243, 337, 1280
MscI TGGCCA 1 cut(s) 704
MseI TTAA 4 cut(s) 195, 539, 753, 966
MslI CAYNNNNRTG 1 cut(s) 94
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 2 cut(s) 1087, 1140
MspR9I CCNGG 2 cut(s) 525, 1088
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 1 cut(s) 525
MwoI GCNNNNNNNGC 3 cut(s) 541, 1043, 1188
NarI GGCGCC 1 cut(s) 511
NciI CCSGG 1 cut(s) 1088
NcoI CCATGG 1 cut(s) 342
NdeII GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
NheI GCTAGC 1 cut(s) 488
NlaIV GGNNCC 6 cut(s) 512, 1085, 1158, 1159, 1171, 1234
NmeAIII GCCGAG 2 cut(s) 316, 961
NmuCI GTSAC 2 cut(s) 473, 576
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 4 cut(s) 213, 299, 822, 926
NspV TTCGAA 1 cut(s) 247
PaqCI CACCTGC 1 cut(s) 486
PciI ACATGT 2 cut(s) 818, 922
PctI GAATGC 1 cut(s) 688
PdmI GAANNNNTTC 3 cut(s) 243, 337, 1280
PfeI GAWTC 4 cut(s) 570, 635, 949, 1109
PfoI TCCNGGA 1 cut(s) 523
PkrI GCNGC 4 cut(s) 25, 133, 429, 866
PleI GAGTC 6 cut(s) 211, 298, 388, 480, 569, 748
PluTI GGCGCC 1 cut(s) 514
PpsI GAGTC 6 cut(s) 211, 298, 388, 480, 569, 748
PpuMI RGGWCCY 1 cut(s) 1157
PscI ACATGT 2 cut(s) 818, 922
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 1157
Psp6I CCWGG 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 979
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 6 cut(s) 512, 1085, 1158, 1159, 1171, 1234
PspPI GGNCC 1 cut(s) 1157
PspPPI RGGWCCY 1 cut(s) 1157
PsuI RGATCY 3 cut(s) 1014, 1096, 1169
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 3 cut(s) 69, 86, 997
RsaNI GTAC 3 cut(s) 68, 85, 996
RseI CAYNNNNRTG 1 cut(s) 94
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 4 cut(s) 195, 539, 753, 966
SatI GCNGC 4 cut(s) 24, 132, 428, 865
Sau3AI GATC 7 cut(s) 185, 316, 320, 1014, 1096, 1123, 1169
Sau96I GGNCC 1 cut(s) 1157
SchI GAGTC 6 cut(s) 212, 298, 389, 481, 570, 749
ScrFI CCNGG 2 cut(s) 525, 1088
SduI GDGCHC 2 cut(s) 650, 699
SfaNI GCATC 3 cut(s) 337, 461, 544
SfoI GGCGCC 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 247
SinI GGWCC 1 cut(s) 1157
SmiMI CAYNNNNRTG 1 cut(s) 94
Sse9I AATT 7 cut(s) 63, 197, 273, 413, 594, 754, 963
SsiI CCGC 1 cut(s) 450
SspDI GGCGCC 1 cut(s) 510
SspI AATATT 2 cut(s) 79, 337
SspMI CTAG 8 cut(s) 27, 54, 489, 642, 771, 911, 1065, 1269
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 2 cut(s) 523, 1086
StyI CCWWGG 2 cut(s) 342, 1194
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 247, 319, 1079
TasI AATT 7 cut(s) 63, 197, 273, 413, 594, 754, 963
TatI WGTACW 2 cut(s) 67, 995
TfiI GAWTC 4 cut(s) 570, 635, 949, 1109
Tru1I TTAA 4 cut(s) 195, 539, 753, 966
Tru9I TTAA 4 cut(s) 195, 539, 753, 966
TscAI CASTG 4 cut(s) 55, 507, 912, 996
TseFI GTSAC 2 cut(s) 473, 576
TseI GCWGC 4 cut(s) 23, 131, 427, 864
Tsp45I GTSAC 2 cut(s) 473, 576
TspDTI ATGAA 5 cut(s) 384, 429, 648, 695, 846
TspRI CASTG 4 cut(s) 55, 507, 912, 996
VpaK11BI GGWCC 1 cut(s) 1157
XapI RAATTY 1 cut(s) 197
XbaI TCTAGA 1 cut(s) 1064
XceI RCATGY 4 cut(s) 213, 299, 822, 926
XmnI GAANNNNTTC 3 cut(s) 243, 337, 1280
XspI CTAG 8 cut(s) 27, 54, 489, 642, 771, 911, 1065, 1269
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.