Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008409

Physical Location & Seq

Genomic Coordinates Reverse (-)

15418243 .. 15428116

Gene Structure 9874 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1073000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGAAGGCCTTGAATAGCATAGAGGATCTGGTCGAGGAGATTAAGATTCGAGTGGTTTGGTGGGCCCTGTTTGTCTTCTGTGTTACATACTTTCTTTCCTATTCGAGTAAATCAATGTGGATGAATTTACCCATATCAATTTTATTGGTTTCAATGCTACGGATACTAATGAATAACGTGGAATTCCGTTGGAAGAGTCAAAAACTTGTTCGTCCACAATCATATTTGTCTCATTTGGAGAAGAAACAGTTGTCATTGAATGATCCACGACTCTCTACTGGGACTCCACCACCAAAATGGAAAAGGAAAATTGGTTCTCCAATCGTAGAGGATGCAGTTAAAGATTTCATTGACAAACTTTTGAAGGATTTTGTGATTGATTTGTGGTATTCAGATATTACTCCGGACAAGGAGGCGCCAGAGCAGATACGCGCGATAATCATGGATGCTCTGGGTGAAGTATCCGGAAGAGTTAAAGAGATAAACCTTGTTGACTTGCTAACAAGGGATATTATTGATTTAATAGGGGATCATATAGAACTTTTTAGAAAAAACCAAGCTGCTATAGGTGTTGATGTTATGAAAACGTTGTCTTCAGAGGAGAGAGATGAAAGGTTAAAACATCATCTCATGGCTTCGAAGGAGCTCCATCCTGCAATGATTTCACCCGAGAGTGAGTACAAGGTTCTTCAGCGGCTAATGGGTGGGGTATTAGCTGTGGTGTTAAGACCACGAGAAGCTCAATGTCCAGTGGTTAGATCTATTGCCAGAGAACTTCTAACAAGCTTAGTAATTCAACCTGTTTTGAGTTTTGCAAGCCCTGGGTATATCAACGAATTGATTGAATACATTTTGCTAGCCATTAAAGAGGAAATCAGTAAAGTGGTAAGTGGGGACCAATCAGCTGCAGGGGTTGTACATGATCAAGATTCTCCACTGAGGAAATCTGCAACCTTTAACCAAAGCACTGTTATGACATTGGCTAAAGGTGACCACCAGAGAGAAGCGTATTCAGATTATAATAAATTCCAGGAAGATCCTTTGCAGCCACGGCCTGCTGATTGGGCACGAGTTTTAGAGGCTGCAACACAGAGAAGGACTGAAGTTCTTGCTCCTGAAAATCTTGAAAATATGTGGACAAAAGGGAGAAATTACAAAAAAAAAGAGCACAAGAAAAGGATAAGAAGAGTTCAGGAACCTATATCTGAGTGTTCTGGAATAGATAGTGCTGTACCTGCTAAAAATCTGGGCAACGAAATGGTAGCCGACAGGCATGAAATTTCTACAGGAATGGAAGATAGATCTATTGTGAAGCTAAGAAGTGAATTAAGTTTAGATACTCAATTAAGTGGTGGGACCAAAATGGAACTACACTACTCTTTAGATCCCAGCGAAGAATCATATGCGGATCAAGGGCATCTTGTTAATAAATTGGAGGACATTGGAAACCTTGCTGCTGATGGAAATAAAAGCAGGCTTAAAAGATCAAATAGCACTTCTGCTTTGAAAATCCAGCCAGACACAAAAAGAACATTGACAGAAGGTGGAGGGTCTATCATATCAGAATTCTATAGCCCTGAGTTTGGCAGGCATAGAGAAGACCATATTAGTAAGAGTGCCTCGGATATGTTAGTCCACTGTGTGGGACAACAAGTTCCCAAGCTCAGGTGCCGGGTTATGGGAGCCTACTTTGAGAAACTTGGGTCAAAATCTTTTGCAGTTTATTCAATTGCAGTTACAGATTCAGAAAACAGAACTTGGTTTGTGAAAAGAAGATATAGGAATTTTGAACGACTGCATCGCCATCTTAAAGACATTCCAAATTACACTTTACATTTACCACCCAAAAGAATATTCTCGTCAAGCACAGAGGATGCTTTTGTTCATCAGCGATGTATTCAGCTAGACAAATATCTACAGGATCTGTTGTCAATAGCTAATGTTGCCGAACAGCATGAAGTCTGGGATTTTTTAAGTGGTTCATCAAAGAATTACTCTTTTGGAAAATCTCCTTCAGTGATGAGAACTCTTGCAGTCAATGTGGATGACGCTGTGGATGACATTGTACGCCAGTTCAAAGGGGTTTCAGATGGGTTAATGCGTAAGGTTGTTGGTTCTCCCATGTCTGAGGCCTCGTCTTTATTTCCTGGATGGAACTTGTCCGGGAATGCAGATGAGACAGGTGTCCTTGCTTTTAGGCAAAATACAGCAGAGTCGACAAATAGCTTTTCAGATAACGAGGAAGGTACCCTAGAAAATGGGTGGCATTCAGACAATGAATTGAACTACAAGGGTTATCCACCTCGGGTTGTACATACAAGGACCTTAGGTTTGGAGAAGAAAGCTGATTTAGTTGGCAAGGGTGGCTTTCTGGAGGATCCAATTGGAATGCCACCTGAGTGGACTCCACCCAATGTTAGCGTGCCTCTGTTGAATCTAGTGGACAAGGTATTTCAACTTAAGAGAAGGGGCTGGCTGAGACGACAGGTCTTTTGGATGTCAAAACAAATATTGCAGTTAATGATGGACGATGCTATTGATGACTGGCTTATGAGGCAGATTCATTGGCTTCGGAGGGAGGATATTATTGCTTCGGGAATCTATTGGCTTAAAGATGTTCTGTGGCCAAATGGCACATTCTTCCTTAGAGTCGGCAATGTTCAAGACGGCAATCAAAACCCTTTTCAAAATATAAGTCAATTTGATGGCAGTAAGGCTGGTAAGCCGGGGTCTTTTGAGCAGCAGCTTGAGGCTGCTCGCAGAGCTAGTGATATCAAGAAAATGCTCTTTGATGGAACTCCGGCGCCCTTGGTTAGCTTAATTGGGCATAAGCAGTACAGACGTTGTGCAAGAGACATCTATTATTTCATTCAGTCTACCATCTGCATAAAGCAACTTGCATACGCAATACTTGAACTGTCACTCGTTTCAATTTTTCCTGAGCTGCGGGATCTTATTGTTGATGTTCATCAAAAGATGGGTGTTGATCAAACTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0006605 GO:0006622 GO:0006623 GO:0006810 GO:0006886 GO:0007034 GO:0007041 GO:0008104 GO:0008150 GO:0008289 GO:0008333 GO:0010008 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016482 GO:0019898 GO:0031090 GO:0031312 GO:0031313 GO:0031410 GO:0031982 GO:0033036 GO:0033365 GO:0034613 GO:0035091 GO:0042802 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045022 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061462 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098805 GO:0098927

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nexin_C PX PXA

Protein Analysis

1000

Amino Acids

113.66

Weight (kDa)

7.65

Isoelectric Point (pI)

48.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PXA	PF02194	106 - 284	1.3e-41	PXA domain
PX	PF00787	582 - 659	1.6e-13	PX domain
Nexin_C	PF08628	825 - 959	5.3e-24	Sorting nexin C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008409)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15900
fragaria_vesca	FvH4_2g07180 FvH4_2g07180 FvH4_2g07180 FvH4_2g07180 FvH4_2g07180 FvH4_2g07180
malus_domestica	MD05G1073000.v1.1 MD10G1087600.v1.1
prunus_persica	Prupe.8G113600_v2.0.a1 Prupe.8G113600_v2.0.a1
pyrus_communis	pycom05g06550 pycom10g06990
rosa_chinensis	RchiOBHm_Chr6g0259621
rosa_laevigata	RLG00000014517
rosa_multiflora	Rmu_sc0000536.1_g000017
rosa_roxburghii	Rroxscaffold_7G00206920
rosa_rugosa	Rorug05G0590000
rosa_samantha	Rh6AG106200 Rh6BG100700 Rh6CG095300 Rh6DG089200
rosa_wichuraiana	Rw6G009180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1020
Acc36I	ACCTGC	1 cut(s)	1243
Acc65I	GGTACC	1 cut(s)	2246
AccB1I	GGYRCC	4 cut(s)	415, 1668, 2246, 2803
AccB7I	CCANNNNNTGG	1 cut(s)	1642
AccI	GTMKAC	2 cut(s)	2216, 2876
AccII	CGCG	2 cut(s)	432, 434
AccIII	TCCGGA	2 cut(s)	403, 464
AciI	CCGC	3 cut(s)	694, 1406, 2947
AclI	AACGTT	1 cut(s)	587
AcoI	YGGCCR	1 cut(s)	2624
AcsI	RAATTY	6 cut(s)	124, 182, 1025, 1278, 1565, 1783
AcuI	CTGAAG	4 cut(s)	579, 674, 1122, 1998
AcyI	GRCGYC	2 cut(s)	416, 2804
AdeI	CACNNNGTG	1 cut(s)	1642
AfaI	GTAC	7 cut(s)	680, 918, 1233, 2067, 2248, 2313, 2837
AfiI	CCNNNNNNNGG	4 cut(s)	1583, 1642, 1665, 1678
AflII	CTTAAG	1 cut(s)	2459
AhdI	GACNNNNNGTC	1 cut(s)	2486
AjnI	CCWGG	3 cut(s)	820, 1029, 2146
AjuI	GAANNNNNNNTTGG	2 cut(s)	1838, 1870
AleI	CACNNNNGTG	1 cut(s)	2398
AloI	GAACNNNNNNTCC	4 cut(s)	298, 330, 1638, 1670
Alw21I	GWGCWC	2 cut(s)	648, 1170
Alw26I	GTCTC	4 cut(s)	234, 2171, 2473, 2847
AlwNI	CAGNNNCTG	1 cut(s)	1924
Ama87I	CYCGRG	2 cut(s)	668, 2304
Aor13HI	TCCGGA	2 cut(s)	403, 464
AoxI	GGCC	5 cut(s)	6, 63, 1052, 2130, 2624
ApaI	GGGCCC	1 cut(s)	67
ApeKI	GCWGC	9 cut(s)	560, 905, 1045, 1082, 1454, 2740, 2743, 2753, 2944
ApoI	RAATTY	6 cut(s)	124, 182, 1025, 1278, 1565, 1783
ArsI	GACNNNNNNTTYG	6 cut(s)	2314, 2346, 2475, 2507, 2680, 2712
Asp718I	GGTACC	1 cut(s)	2246
AspLEI	GCGC	3 cut(s)	418, 434, 2806
AspS9I	GGNCC	5 cut(s)	63, 64, 895, 1356, 2322
AsuC2I	CCSGG	3 cut(s)	1673, 2164, 2727
AsuHPI	GGTGA	3 cut(s)	467, 657, 1001
AsuII	TTCGAA	1 cut(s)	638
AsuNHI	GCTAGC	1 cut(s)	856
AvaI	CYCGRG	2 cut(s)	668, 2304
AvaII	GGWCC	3 cut(s)	895, 1356, 2322
AxyI	CCTNAGG	1 cut(s)	2326
BaeGI	GKGCMC	2 cut(s)	67, 1069
BalI	TGGCCA	1 cut(s)	2626
BamHI	GGATCC	1 cut(s)	2377
BanI	GGYRCC	4 cut(s)	415, 1668, 2246, 2803
BanII	GRGCYC	2 cut(s)	67, 648
BauI	CACGAG	2 cut(s)	732, 1068
BbsI	GAAGAC	3 cut(s)	67, 585, 1605
Bbv12I	GWGCWC	2 cut(s)	648, 1170
BbvI	GCAGC	9 cut(s)	547, 892, 1057, 1069, 1441, 2740, 2752, 2755, 2931
BceAI	ACGGC	2 cut(s)	1067, 2683
BciT130I	CCWGG	3 cut(s)	822, 1031, 2148
BciVI	GTATCC	2 cut(s)	156, 472
BclI	TGATCA	2 cut(s)	922, 2986
BcnI	CCSGG	3 cut(s)	1673, 2164, 2727
BcoDI	GTCTC	4 cut(s)	234, 2171, 2473, 2847
BfaI	CTAG	5 cut(s)	857, 1904, 2252, 2438, 2766
BfmI	CTRYAG	5 cut(s)	564, 906, 1284, 1570, 1916
BfoI	RGCGCY	2 cut(s)	419, 2807
BfrI	CTTAAG	1 cut(s)	2459
BfuAI	ACCTGC	1 cut(s)	1243
BfuI	GTATCC	2 cut(s)	156, 472
BglII	AGATCT	2 cut(s)	758, 1301
Bme1390I	CCNGG	6 cut(s)	822, 1031, 1673, 2148, 2164, 2727
Bme18I	GGWCC	3 cut(s)	895, 1356, 2322
BmeRI	GACNNNNNGTC	1 cut(s)	2486
BmeT110I	CYCGRG	2 cut(s)	668, 2304
BmgT120I	GGNCC	5 cut(s)	63, 64, 895, 1356, 2322
BmrFI	CCNGG	6 cut(s)	822, 1031, 1673, 2148, 2164, 2727
BmrI	ACTGGG	1 cut(s)	288
BmsI	GCATC	6 cut(s)	322, 436, 1426, 1807, 1864, 2521
BmtI	GCTAGC	1 cut(s)	860
BmuI	ACTGGG	1 cut(s)	288
BoxI	GACNNNNGTC	1 cut(s)	2183
BpiI	GAAGAC	3 cut(s)	67, 585, 1605
BpmI	CTGGAG	1 cut(s)	2393
Bpu10I	CCTNAGC	2 cut(s)	1664, 2940
Bpu14I	TTCGAA	1 cut(s)	638
BpuEI	CTTGAG	1 cut(s)	2768
BpuMI	CCSGG	3 cut(s)	1673, 2164, 2727
BsaBI	GATNNNNATC	1 cut(s)	2967
BsaHI	GRCGYC	2 cut(s)	416, 2804
BsaJI	CCNNGG	7 cut(s)	820, 821, 1049, 1620, 2303, 2726, 2808
BsaWI	WCCGGW	2 cut(s)	403, 464
BsaXI	ACNNNNNCTCC	2 cut(s)	268, 298
Bsc4I	CCNNNNNNNGG	4 cut(s)	1583, 1642, 1665, 1678
Bse1I	ACTGG	4 cut(s)	283, 749, 2071, 2549
Bse21I	CCTNAGG	1 cut(s)	2326
Bse3DI	GCAATG	2 cut(s)	663, 2662
Bse8I	GATNNNNATC	1 cut(s)	2967
BseAI	TCCGGA	2 cut(s)	403, 464
BseBI	CCWGG	3 cut(s)	822, 1031, 2148
BseDI	CCNNGG	7 cut(s)	820, 821, 1049, 1620, 2303, 2726, 2808
BseGI	GGATG	9 cut(s)	126, 337, 451, 649, 1879, 2050, 2062, 2156, 2502
BseJI	GATNNNNATC	1 cut(s)	2967
BseLI	CCNNNNNNNGG	4 cut(s)	1583, 1642, 1665, 1678
BseMI	GCAATG	2 cut(s)	663, 2662
BseMII	CTCAG	8 cut(s)	929, 1197, 1569, 1678, 2118, 2388, 2468, 2931
BseNI	ACTGG	4 cut(s)	283, 749, 2071, 2549
BseRI	GAGGAG	2 cut(s)	50, 614
BseSI	GKGCMC	2 cut(s)	67, 1069
BseXI	GCAGC	9 cut(s)	547, 892, 1057, 1069, 1441, 2740, 2752, 2755, 2931
BseYI	CCCAGC	1 cut(s)	1388
Bsh1236I	CGCG	2 cut(s)	432, 434
BshFI	GGCC	5 cut(s)	8, 65, 1054, 2132, 2626
BshNI	GGYRCC	4 cut(s)	415, 1668, 2246, 2803
BsiHKAI	GWGCWC	2 cut(s)	648, 1170
BsiHKCI	CYCGRG	2 cut(s)	668, 2304
BsiSI	CCGG	6 cut(s)	404, 465, 1672, 2163, 2726, 2801
BslFI	GGGAC	4 cut(s)	295, 908, 1369, 1659
BslI	CCNNNNNNNGG	4 cut(s)	1583, 1642, 1665, 1678
BsmAI	GTCTC	4 cut(s)	234, 2171, 2473, 2847
BsmBI	CGTCTC	1 cut(s)	2473
BsmFI	GGGAC	4 cut(s)	295, 908, 1369, 1659
BsmI	GAATGC	3 cut(s)	2173, 2266, 2394
BsnI	GGCC	5 cut(s)	8, 65, 1054, 2132, 2626
BsoBI	CYCGRG	2 cut(s)	668, 2304
Bsp119I	TTCGAA	1 cut(s)	638
Bsp120I	GGGCCC	1 cut(s)	63
Bsp1286I	GDGCHC	4 cut(s)	67, 648, 1069, 1170
Bsp13I	TCCGGA	2 cut(s)	403, 464
Bsp1407I	TGTACA	2 cut(s)	916, 2311
BspACI	CCGC	3 cut(s)	694, 1406, 2947
BspANI	GGCC	5 cut(s)	8, 65, 1054, 2132, 2626
BspCNI	CTCAG	8 cut(s)	930, 1198, 1570, 1677, 2119, 2389, 2469, 2932
BspEI	TCCGGA	2 cut(s)	403, 464
BspFNI	CGCG	2 cut(s)	432, 434
BspMAI	CTGCAG	1 cut(s)	910
BspMI	ACCTGC	1 cut(s)	1243
BspOI	GCTAGC	1 cut(s)	860
BspT104I	TTCGAA	1 cut(s)	638
BspT107I	GGYRCC	4 cut(s)	415, 1668, 2246, 2803
BspTI	CTTAAG	1 cut(s)	2459
BsrDI	GCAATG	2 cut(s)	663, 2662
BsrGI	TGTACA	2 cut(s)	916, 2311
BsrI	ACTGG	4 cut(s)	283, 749, 2071, 2549
BssECI	CCNNGG	7 cut(s)	820, 821, 1049, 1620, 2303, 2726, 2808
BssNI	GRCGYC	2 cut(s)	416, 2804
BssSI	CACGAG	2 cut(s)	732, 1068
BssT1I	CCWWGG	1 cut(s)	2808
Bst2BI	CACGAG	2 cut(s)	732, 1068
Bst2UI	CCWGG	3 cut(s)	822, 1031, 2148
Bst4CI	ACNGT	5 cut(s)	249, 970, 1640, 2919, 2995
Bst6I	CTCTTC	3 cut(s)	188, 463, 1180
BstACI	GRCGYC	2 cut(s)	416, 2804
BstAFI	CTTAAG	1 cut(s)	2459
BstAUI	TGTACA	2 cut(s)	916, 2311
BstBI	TTCGAA	1 cut(s)	638
BstC8I	GCNNGC	8 cut(s)	817, 858, 1056, 1475, 1589, 2423, 2474, 2758
BstDSI	CCRYGG	1 cut(s)	1049
BstEII	GGTNACC	1 cut(s)	989
BstF5I	GGATG	9 cut(s)	126, 337, 451, 649, 1879, 2050, 2062, 2156, 2502
BstFNI	CGCG	2 cut(s)	432, 434
BstH2I	RGCGCY	2 cut(s)	419, 2807
BstHHI	GCGC	3 cut(s)	418, 434, 2806
BstMAI	GTCTC	4 cut(s)	234, 2171, 2473, 2847
BstMWI	GCNNNNNNNGC	7 cut(s)	1051, 1064, 1235, 1943, 2364, 2554, 2762
BstNI	CCWGG	3 cut(s)	822, 1031, 2148
BstPAI	GACNNNNGTC	1 cut(s)	2183
BstPI	GGTNACC	1 cut(s)	989
BstSCI	CCNGG	6 cut(s)	820, 1029, 1671, 2146, 2162, 2725
BstSFI	CTRYAG	5 cut(s)	564, 906, 1284, 1570, 1916
BstSLI	GKGCMC	2 cut(s)	67, 1069
BstUI	CGCG	2 cut(s)	432, 434
BstV1I	GCAGC	9 cut(s)	547, 892, 1057, 1069, 1441, 2740, 2752, 2755, 2931
BstV2I	GAAGAC	3 cut(s)	67, 585, 1605
BstX2I	RGATCY	8 cut(s)	25, 758, 1036, 1301, 1384, 1921, 2377, 2950
BstXI	CCANNNNNNTGG	2 cut(s)	297, 2400
BstYI	RGATCY	8 cut(s)	25, 758, 1036, 1301, 1384, 1921, 2377, 2950
Bsu36I	CCTNAGG	1 cut(s)	2326
BsuI	GTATCC	2 cut(s)	156, 472
BsuRI	GGCC	5 cut(s)	8, 65, 1054, 2132, 2626
BtgI	CCRYGG	1 cut(s)	1049
BtgZI	GCGATG	2 cut(s)	1784, 1906
BtsCI	GGATG	9 cut(s)	126, 337, 451, 649, 1879, 2050, 2062, 2156, 2502
BtsIMutI	CAGTG	5 cut(s)	756, 935, 966, 1636, 2022
BveI	ACCTGC	1 cut(s)	1243
Cac8I	GCNNGC	8 cut(s)	817, 858, 1056, 1475, 1589, 2423, 2474, 2758
CaiI	CAGNNNCTG	1 cut(s)	1924
CfoI	GCGC	3 cut(s)	418, 434, 2806
Cfr13I	GGNCC	5 cut(s)	63, 64, 895, 1356, 2322
CseI	GACGC	1 cut(s)	2057
Csp6I	GTAC	7 cut(s)	679, 917, 1232, 2066, 2247, 2312, 2836
CviAII	CATG	6 cut(s)	442, 631, 920, 1274, 1955, 2122
CviQI	GTAC	7 cut(s)	679, 917, 1232, 2066, 2247, 2312, 2836
DinI	GGCGCC	2 cut(s)	417, 2805
DraIII	CACNNNGTG	1 cut(s)	1642
DriI	GACNNNNNGTC	1 cut(s)	2486
EaeI	YGGCCR	1 cut(s)	2624
Eam1104I	CTCTTC	3 cut(s)	188, 463, 1180
Eam1105I	GACNNNNNGTC	1 cut(s)	2486
EarI	CTCTTC	3 cut(s)	188, 463, 1180
Ecl136II	GAGCTC	1 cut(s)	646
Eco130I	CCWWGG	1 cut(s)	2808
Eco147I	AGGCCT	2 cut(s)	8, 2132
Eco24I	GRGCYC	2 cut(s)	67, 648
Eco32I	GATATC	1 cut(s)	2773
Eco47I	GGWCC	3 cut(s)	895, 1356, 2322
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	4 cut(s)	579, 674, 1122, 1998
Eco81I	CCTNAGG	1 cut(s)	2326
Eco88I	CYCGRG	2 cut(s)	668, 2304
Eco91I	GGTNACC	1 cut(s)	989
EcoICRI	GAGCTC	1 cut(s)	646
EcoO109I	RGGNCCY	2 cut(s)	64, 2322
EcoO65I	GGTNACC	1 cut(s)	989
EcoRI	GAATTC	2 cut(s)	182, 1565
EcoRII	CCWGG	3 cut(s)	820, 1029, 2146
EcoRV	GATATC	1 cut(s)	2773
EcoT14I	CCWWGG	1 cut(s)	2808
EcoT38I	GRGCYC	2 cut(s)	67, 648
EgeI	GGCGCC	2 cut(s)	417, 2805
EheI	GGCGCC	2 cut(s)	417, 2805
ErhI	CCWWGG	1 cut(s)	2808
Esp3I	CGTCTC	1 cut(s)	2473
FaeI	CATG	6 cut(s)	445, 634, 923, 1277, 1958, 2125
FalI	AAGNNNNNCTT	4 cut(s)	1404, 1436, 2351, 2383
FaqI	GGGAC	4 cut(s)	295, 908, 1369, 1659
FatI	CATG	6 cut(s)	441, 630, 919, 1273, 1954, 2121
FauI	CCCGC	1 cut(s)	2940
FauNDI	CATATG	1 cut(s)	1402
FbaI	TGATCA	2 cut(s)	922, 2986
FblI	GTMKAC	2 cut(s)	2216, 2876
FokI	GGATG	9 cut(s)	133, 344, 458, 636, 1886, 2057, 2069, 2163, 2509
FriOI	GRGCYC	2 cut(s)	67, 648
FspBI	CTAG	5 cut(s)	857, 1904, 2252, 2438, 2766
GlaI	GCGC	3 cut(s)	417, 433, 2805
GsaI	CCCAGC	1 cut(s)	1392
GsuI	CTGGAG	1 cut(s)	2393
HaeII	RGCGCY	2 cut(s)	419, 2807
HaeIII	GGCC	5 cut(s)	8, 65, 1054, 2132, 2626
HapII	CCGG	6 cut(s)	404, 465, 1672, 2163, 2726, 2801
HgaI	GACGC	1 cut(s)	2057
HhaI	GCGC	3 cut(s)	418, 434, 2806
Hin1I	GRCGYC	2 cut(s)	416, 2804
Hin1II	CATG	6 cut(s)	445, 634, 923, 1277, 1958, 2125
Hin6I	GCGC	3 cut(s)	416, 432, 2804
HinP1I	GCGC	3 cut(s)	416, 432, 2804
HincII	GTYRAC	2 cut(s)	493, 2217
HindII	GTYRAC	2 cut(s)	493, 2217
HindIII	AAGCTT	1 cut(s)	784
HpaII	CCGG	6 cut(s)	404, 465, 1672, 2163, 2726, 2801
HphI	GGTGA	3 cut(s)	467, 657, 1001
Hpy166II	GTNNAC	8 cut(s)	215, 493, 1137, 1636, 2217, 2403, 2443, 2877
Hpy8I	GTNNAC	8 cut(s)	215, 493, 1137, 1636, 2217, 2403, 2443, 2877
HpyAV	CCTTC	7 cut(s)	358, 634, 1089, 1535, 2022, 2237, 2460
HpyCH4III	ACNGT	5 cut(s)	249, 970, 1640, 2919, 2995
HpyCH4IV	ACGT	3 cut(s)	177, 587, 2842
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1051, 1064, 1235, 1943, 2364, 2554, 2762
HpySE526I	ACGT	3 cut(s)	177, 587, 2842
Hsp92I	GRCGYC	2 cut(s)	416, 2804
Hsp92II	CATG	6 cut(s)	445, 634, 923, 1277, 1958, 2125
HspAI	GCGC	3 cut(s)	416, 432, 2804
KasI	GGCGCC	2 cut(s)	415, 2803
Kpn2I	TCCGGA	2 cut(s)	403, 464
KpnI	GGTACC	1 cut(s)	2250
Ksp22I	TGATCA	2 cut(s)	922, 2986
LmnI	GCTCC	4 cut(s)	643, 651, 1117, 1682
Lsp1109I	GCAGC	9 cut(s)	547, 892, 1057, 1069, 1441, 2740, 2752, 2755, 2931
LweI	GCATC	6 cut(s)	322, 436, 1426, 1807, 1864, 2521
MaeI	CTAG	5 cut(s)	857, 1904, 2252, 2438, 2766
MaeII	ACGT	3 cut(s)	177, 587, 2842
MaeIII	GTNAC	4 cut(s)	82, 989, 1735, 2919
MfeI	CAATTG	2 cut(s)	1728, 2382
MflI	RGATCY	8 cut(s)	25, 758, 1036, 1301, 1384, 1921, 2377, 2950
MhlI	GDGCHC	4 cut(s)	67, 648, 1069, 1170
MlsI	TGGCCA	1 cut(s)	2626
MluNI	TGGCCA	1 cut(s)	2626
Mly113I	GGCGCC	2 cut(s)	416, 2804
MlyI	GAGTC	6 cut(s)	205, 264, 277, 2222, 2398, 2658
MmeI	TCCRAC	1 cut(s)	170
Mox20I	TGGCCA	1 cut(s)	2626
MroI	TCCGGA	2 cut(s)	403, 464
MscI	TGGCCA	1 cut(s)	2626
MslI	CAYNNNNRTG	3 cut(s)	295, 971, 2398
Msp20I	TGGCCA	1 cut(s)	2626
MspA1I	CMGCKG	2 cut(s)	694, 905
MspCI	CTTAAG	1 cut(s)	2459
MspI	CCGG	6 cut(s)	404, 465, 1672, 2163, 2726, 2801
MspR9I	CCNGG	6 cut(s)	822, 1031, 1673, 2148, 2164, 2727
MunI	CAATTG	2 cut(s)	1728, 2382
Mva1269I	GAATGC	3 cut(s)	2173, 2266, 2394
MvaI	CCWGG	3 cut(s)	822, 1031, 2148
MvnI	CGCG	2 cut(s)	432, 434
MwoI	GCNNNNNNNGC	7 cut(s)	1051, 1064, 1235, 1943, 2364, 2554, 2762
NarI	GGCGCC	2 cut(s)	416, 2804
NciI	CCSGG	3 cut(s)	1673, 2164, 2727
NdeI	CATATG	1 cut(s)	1402
NheI	GCTAGC	1 cut(s)	856
NlaIII	CATG	6 cut(s)	445, 634, 923, 1277, 1958, 2125
NmuCI	GTSAC	2 cut(s)	989, 2919
NspV	TTCGAA	1 cut(s)	638
OliI	CACNNNNGTG	1 cut(s)	2398
PasI	CCCWGGG	1 cut(s)	821
PceI	AGGCCT	2 cut(s)	8, 2132
PctI	GAATGC	3 cut(s)	2173, 2266, 2394
PfeI	GAWTC	7 cut(s)	46, 929, 1397, 1742, 2434, 2560, 2598
PflMI	CCANNNNNTGG	1 cut(s)	1642
PfoI	TCCNGGA	3 cut(s)	1029, 2146, 2162
PleI	GAGTC	6 cut(s)	204, 264, 277, 2221, 2398, 2657
PluTI	GGCGCC	2 cut(s)	419, 2807
PpsI	GAGTC	6 cut(s)	204, 264, 277, 2221, 2398, 2657
PpuMI	RGGWCCY	1 cut(s)	2322
PshAI	GACNNNNGTC	1 cut(s)	2183
PsiI	TTATAA	1 cut(s)	1020
Psp124BI	GAGCTC	1 cut(s)	648
Psp1406I	AACGTT	1 cut(s)	587
Psp5II	RGGWCCY	1 cut(s)	2322
Psp6I	CCWGG	3 cut(s)	820, 1029, 2146
PspEI	GGTNACC	1 cut(s)	989
PspFI	CCCAGC	1 cut(s)	1388
PspGI	CCWGG	3 cut(s)	820, 1029, 2146
PspOMI	GGGCCC	1 cut(s)	63
PspPI	GGNCC	5 cut(s)	63, 64, 895, 1356, 2322
PspPPI	RGGWCCY	1 cut(s)	2322
PstI	CTGCAG	1 cut(s)	910
PstNI	CAGNNNCTG	1 cut(s)	1924
PsuI	RGATCY	8 cut(s)	25, 758, 1036, 1301, 1384, 1921, 2377, 2950
PvuII	CAGCTG	1 cut(s)	905
RsaI	GTAC	7 cut(s)	680, 918, 1233, 2067, 2248, 2313, 2837
RsaNI	GTAC	7 cut(s)	679, 917, 1232, 2066, 2247, 2312, 2836
RseI	CAYNNNNRTG	3 cut(s)	295, 971, 2398
SacI	GAGCTC	1 cut(s)	648
SalI	GTCGAC	1 cut(s)	2215
Sau96I	GGNCC	5 cut(s)	63, 64, 895, 1356, 2322
SchI	GAGTC	6 cut(s)	205, 264, 277, 2222, 2398, 2658
ScrFI	CCNGG	6 cut(s)	822, 1031, 1673, 2148, 2164, 2727
SduI	GDGCHC	4 cut(s)	67, 648, 1069, 1170
SfaNI	GCATC	6 cut(s)	322, 436, 1426, 1807, 1864, 2521
SfcI	CTRYAG	5 cut(s)	564, 906, 1284, 1570, 1916
SfoI	GGCGCC	2 cut(s)	417, 2805
SfuI	TTCGAA	1 cut(s)	638
SinI	GGWCC	3 cut(s)	895, 1356, 2322
SmiMI	CAYNNNNRTG	3 cut(s)	295, 971, 2398
SmlI	CTYRAG	2 cut(s)	2459, 2747
SmoI	CTYRAG	2 cut(s)	2459, 2747
SseBI	AGGCCT	2 cut(s)	8, 2132
SsiI	CCGC	3 cut(s)	694, 1406, 2947
SspDI	GGCGCC	2 cut(s)	415, 2803
SspI	AATATT	2 cut(s)	1854, 2511
SspMI	CTAG	5 cut(s)	857, 1904, 2252, 2438, 2766
SstI	GAGCTC	1 cut(s)	648
StuI	AGGCCT	2 cut(s)	8, 2132
StyD4I	CCNGG	6 cut(s)	820, 1029, 1671, 2146, 2162, 2725
StyI	CCWWGG	1 cut(s)	2808
TaaI	ACNGT	5 cut(s)	249, 970, 1640, 2919, 2995
TaiI	ACGT	3 cut(s)	180, 590, 2845
TaqI	TCGA	5 cut(s)	33, 49, 104, 638, 2216
TatI	WGTACW	4 cut(s)	678, 916, 2311, 2835
TauI	GCSGC	1 cut(s)	697
TfiI	GAWTC	7 cut(s)	46, 929, 1397, 1742, 2434, 2560, 2598
TscAI	CASTG	5 cut(s)	756, 942, 973, 1643, 2022
TseFI	GTSAC	2 cut(s)	989, 2919
TseI	GCWGC	9 cut(s)	560, 905, 1045, 1082, 1454, 2740, 2743, 2753, 2944
Tsp45I	GTSAC	2 cut(s)	989, 2919
TspGWI	ACGGA	2 cut(s)	175, 176
TspRI	CASTG	5 cut(s)	756, 942, 973, 1643, 2022
Van91I	CCANNNNNTGG	1 cut(s)	1642
Vha464I	CTTAAG	1 cut(s)	2459
VpaK11BI	GGWCC	3 cut(s)	895, 1356, 2322
XapI	RAATTY	6 cut(s)	124, 182, 1025, 1278, 1565, 1783
XcmI	CCANNNNNNNNNTGG	1 cut(s)	294
XmiI	GTMKAC	2 cut(s)	2216, 2876
XspI	CTAG	5 cut(s)	857, 1904, 2252, 2438, 2766

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.