MD05G1089200.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004183
Physical Location & Seq
Forward (+)
18960717 .. 18961357
641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1089200.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGCTCTGATAGTAGTGATTTGGGATTATCGATGCAAGAAACTTACGGATAAATGTATCACTGCTCTATTTGATGGCTCTTCCAAGTTGGAGTTGCAAAAATTAGACTTGTCCAATCTTCCTAATCTCTCCGATAATGCAGTTTTATTACTGGCAAAGAGTAGGATTCCGCTACTTGAACTGCGCATGCGGCAATGCCCACTCATAGGTGACACTTCAGTAATGGCATTAGCATCTATGCAAGTTGACGAGGAGAGGTGGCATGGTAACTGCTTAAGGTTGTTGGATCTGTTTAATTGTGGGATTACCCCGCTTTCGTTTCGGTGGTTGAAGAAACCATATTTTCCAAGGCTGAGAATCAGATGGTTGGGAGTTACGGGTAGTGTAAATAGAGACATGGTAGATGCTTTGGCTCCAAGTGGACCGTTCTTGCATGTATCGTGCCATGGTGAGAAGCTGGGAGTTGAGCAAGGGGATACTGCCGATGATGTTTACATGCACGACTTTGATGAGATGGACGAGCTTGAACAGTGGCTTGCAGAAGATGATTATGTTGATGAAGATATGGTGAACGCAGAGGCAGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

197

Amino Acids

22.33

Weight (kDa)

4.68

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 11 - 128 3.2e-20 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 185
AciI CCGC 3 cut(s) 170, 190, 311
AclWI GGATC 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 206
AflII CTTAAG 1 cut(s) 274
AgsI TTSAA 3 cut(s) 179, 331, 527
AluBI AGCT 2 cut(s) 457, 523
AluI AGCT 2 cut(s) 457, 523
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 221, 461, 580
AvaII GGWCC 1 cut(s) 422
BccI CCATC 3 cut(s) 68, 357, 508
BciVI GTATCC 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 387
BfrI CTTAAG 1 cut(s) 274
BfuI GTATCC 1 cut(s) 469
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 3 cut(s) 23, 242, 394
Bsa29I ATCGAT 1 cut(s) 31
BsaJI CCNNGG 2 cut(s) 347, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 347, 445
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 200
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 1 cut(s) 156
BseRI GAGGAG 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 457
BshVI ATCGAT 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 286
Bsp19I CCATGG 1 cut(s) 445
BspACI CCGC 3 cut(s) 170, 190, 311
BspCNI CTCAG 1 cut(s) 345
BspDI ATCGAT 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 85
BspTI CTTAAG 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 200
BsrI ACTGG 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 347, 445
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 2 cut(s) 347, 445
Bst4CI ACNGT 2 cut(s) 426, 531
Bst6I CTCTTC 1 cut(s) 85
BstAFI CTTAAG 1 cut(s) 274
BstC8I GCNNGC 2 cut(s) 188, 537
BstDEI CTNAG 1 cut(s) 353
BstDSI CCRYGG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNSI RCATGY 3 cut(s) 190, 437, 499
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 31
BsuI GTATCC 1 cut(s) 469
BsuTUI ATCGAT 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 445
BtsI GCAGTG 2 cut(s) 60, 588
BtsIMutI CAGTG 3 cut(s) 60, 536, 588
Cac8I GCNNGC 2 cut(s) 188, 537
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 422
ClaI ATCGAT 1 cut(s) 31
CviAII CATG 6 cut(s) 187, 263, 397, 434, 446, 496
CviJI RGCY 7 cut(s) 5, 78, 352, 413, 457, 523, 535
CviKI_1 RGCY 7 cut(s) 5, 78, 352, 413, 457, 523, 535
DdeI CTNAG 1 cut(s) 353
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 347, 445
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 347, 445
ErhI CCWWGG 2 cut(s) 347, 445
FaeI CATG 6 cut(s) 190, 266, 400, 437, 449, 499
FatI CATG 6 cut(s) 186, 262, 396, 433, 445, 495
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 191
FspI TGCGCA 1 cut(s) 185
GlaI GCGC 1 cut(s) 185
GluI GCNGC 1 cut(s) 191
GsaI CCCAGC 1 cut(s) 461
HhaI GCGC 1 cut(s) 186
Hin1II CATG 6 cut(s) 190, 266, 400, 437, 449, 499
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 166, 357
HphI GGTGA 3 cut(s) 221, 461, 580
Hpy166II GTNNAC 4 cut(s) 247, 422, 493, 571
Hpy188I TCNGA 3 cut(s) 9, 133, 362
Hpy8I GTNNAC 4 cut(s) 247, 422, 493, 571
HpyCH4III ACNGT 2 cut(s) 426, 531
HpyCH4V TGCA 7 cut(s) 36, 97, 140, 241, 433, 499, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 6 cut(s) 190, 266, 400, 437, 449, 499
HspAI GCGC 1 cut(s) 184
Kzo9I GATC 1 cut(s) 286
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 2 cut(s) 137, 443
LweI GCATC 3 cut(s) 23, 242, 394
MaeIII GTNAC 3 cut(s) 209, 266, 373
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 5 cut(s) 72, 110, 343, 554, 572
MflI RGATCY 1 cut(s) 286
MluCI AATT 2 cut(s) 101, 295
MmeI TCCRAC 2 cut(s) 69, 264
MnlI CCTC 3 cut(s) 244, 249, 571
MseI TTAA 2 cut(s) 275, 294
MspCI CTTAAG 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 190
NcoI CCATGG 1 cut(s) 445
NdeII GATC 1 cut(s) 286
NlaIII CATG 6 cut(s) 190, 266, 400, 437, 449, 499
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 209
NsbI TGCGCA 1 cut(s) 185
NspI RCATGY 3 cut(s) 190, 437, 499
PaeI GCATGC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 166, 357
PkrI GCNGC 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 457
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 422
PsuI RGATCY 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 275, 294
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 1 cut(s) 422
SetI ASST 5 cut(s) 211, 260, 281, 459, 525
SfaNI GCATC 3 cut(s) 23, 242, 394
SinI GGWCC 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SphI GCATGC 1 cut(s) 190
Sse9I AATT 2 cut(s) 101, 295
SsiI CCGC 3 cut(s) 170, 190, 311
StyI CCWWGG 2 cut(s) 347, 445
TaaI ACNGT 2 cut(s) 426, 531
TaqI TCGA 1 cut(s) 31
TasI AATT 2 cut(s) 101, 295
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 2 cut(s) 166, 357
Tru1I TTAA 2 cut(s) 275, 294
Tru9I TTAA 2 cut(s) 275, 294
TscAI CASTG 3 cut(s) 67, 536, 588
TseFI GTSAC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 573
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 3 cut(s) 67, 536, 588
Vha464I CTTAAG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 422
XceI RCATGY 3 cut(s) 190, 437, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.