MD10G1130400.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004183
Physical Location & Seq
Reverse (-)
21093779 .. 21095997
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1130400.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTTTGATGTATCATCAATCTATCTCCGTCATAATTTCGCTCAAGTTTGGGCTTTGGCGTCGGATAAACTCACGTCCCTCGAGATTGGCTACATTTCATCATTAATGGTGACTGAACTACTTTGCCCAAATTTTGAATCCCATCAATCATCAAACCGTATTCAGCCTTCAATATTGCCAAACATTCACAAATTGTGCCTTTCAGTTGACTATATAACTGATGCTATGGTTGGAACGATTTCTAAAGTGACTAGTTTGGCGTCTAACAAGGATCTGGAAGTTTTGGATTTGAGAGATTGCAGAAACCTCGGGGATGAATCCCTCCGAGCCATAAGCACTCTTCCAAAAGTAAGGACGTCGTTGCTAGATGGCTCTGATATTAGTGATGTGGGATTATCGTATTTGAGACCAGTGGTTGTAAGTACACTAATTTCATTGTCTGTTAGAGGATGCAAGAAACTTACGGATAAATGTATCTCTGCTCTATTTGATGGCTCTTCCAAGTTGGAGTTGCAAGAATTAGACTTGTCCAATCTTCCTTATCTCTCCGATAATGCAGTTTTATTACTGGCAAAGAGTAGGATTCCGCCGCTACTTGAACTGCGCATGCGGCAATGCCCACTCATAGGTGACACTTCAGTAATGGCATTAGCATCTATGCAAATTGACGAGGAGAGGTGGCATGGTAACTGCTTAAGGTTGTTGGATCTGTTTAACTGTGGCGGGATATACCCTGCTTTCGTTTCGGTGGTTGAAGAAACCATATTTTCCAAGGCTGAGATGGTTGGGAGTTACGGACCGTTCTTGCATGTATCGTGCCATGGTGAAGAGCTGGGAGTTGAGCAAGGGGATACTGCCGATGACGTTTACATGCAAGACTTTGATGAGATGGACGAGCTTGAACAGTGGCTTGCAGAAGATGATTATGTTGATGAAGATATGGTGAACGCAGAGGCAGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

322

Amino Acids

35.64

Weight (kDa)

4.41

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 83 - 257 2.5e-33 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
Acc16I TGCGCA 1 cut(s) 605
AciI CCGC 4 cut(s) 587, 590, 610, 723
AclWI GGATC 2 cut(s) 279, 714
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 621
AcyI GRCGYC 3 cut(s) 59, 260, 356
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 626
AflII CTTAAG 1 cut(s) 694
AgsI TTSAA 5 cut(s) 137, 171, 599, 755, 902
AhlI ACTAGT 1 cut(s) 251
AjiI CACGTC 1 cut(s) 75
AluBI AGCT 2 cut(s) 832, 898
AluI AGCT 2 cut(s) 832, 898
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 2 cut(s) 279, 714
Ama87I CYCGRG 2 cut(s) 80, 308
ApoI RAATTY 1 cut(s) 130
AseI ATTAAT 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 606
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 4 cut(s) 121, 641, 836, 955
AvaI CYCGRG 2 cut(s) 80, 308
AvaII GGWCC 1 cut(s) 797
BarI GAAGNNNNNNTAC 2 cut(s) 151, 183
BccI CCATC 5 cut(s) 150, 362, 485, 775, 883
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciVI GTATCC 1 cut(s) 844
BcoDI GTCTC 1 cut(s) 400
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 2 cut(s) 252, 365
BfrI CTTAAG 1 cut(s) 694
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 2 cut(s) 590, 611
BlsI GCNGC 2 cut(s) 591, 612
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 2 cut(s) 80, 308
BmgBI CACGTC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 797
BmsI GCATC 3 cut(s) 211, 440, 662
BpuEI CTTGAG 1 cut(s) 27
BsaHI GRCGYC 3 cut(s) 59, 260, 356
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 307, 771, 820
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 410, 573
Bse3DI GCAATG 1 cut(s) 620
BseDI CCNNGG 3 cut(s) 307, 771, 820
BseGI GGATG 2 cut(s) 319, 455
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 2 cut(s) 410, 573
BseRI GAGGAG 1 cut(s) 686
BseYI CCCAGC 1 cut(s) 832
BsiHKCI CYCGRG 2 cut(s) 80, 308
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 626
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 61
Bso31I GGTCTC 1 cut(s) 400
BsoBI CYCGRG 2 cut(s) 80, 308
Bsp143I GATC 2 cut(s) 271, 706
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 4 cut(s) 587, 590, 610, 723
BspCNI CTCAG 1 cut(s) 769
BspPI GGATC 2 cut(s) 279, 714
BspQI GCTCTTC 2 cut(s) 502, 822
BspTI CTTAAG 1 cut(s) 694
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 620
BsrI ACTGG 2 cut(s) 410, 573
BssECI CCNNGG 3 cut(s) 307, 771, 820
BssMI GATC 2 cut(s) 271, 706
BssNI GRCGYC 3 cut(s) 59, 260, 356
BssT1I CCWWGG 2 cut(s) 771, 820
Bst4CI ACNGT 4 cut(s) 158, 719, 801, 906
Bst6I CTCTTC 3 cut(s) 345, 502, 822
BstACI GRCGYC 3 cut(s) 59, 260, 356
BstAFI CTTAAG 1 cut(s) 694
BstC8I GCNNGC 2 cut(s) 608, 912
BstDEI CTNAG 1 cut(s) 777
BstDSI CCRYGG 1 cut(s) 820
BstF5I GGATG 2 cut(s) 319, 455
BstHHI GCGC 1 cut(s) 606
BstKTI GATC 2 cut(s) 274, 709
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 2 cut(s) 271, 706
BstMWI GCNNNNNNNGC 1 cut(s) 610
BstNSI RCATGY 3 cut(s) 610, 812, 874
BstX2I RGATCY 2 cut(s) 271, 706
BstYI RGATCY 2 cut(s) 271, 706
BsuI GTATCC 1 cut(s) 844
BtgI CCRYGG 1 cut(s) 820
BtrI CACGTC 1 cut(s) 75
BtsCI GGATG 2 cut(s) 319, 455
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 3 cut(s) 417, 911, 963
Cac8I GCNNGC 2 cut(s) 608, 912
CfoI GCGC 1 cut(s) 606
Cfr13I GGNCC 1 cut(s) 797
CpoI CGGWCCG 1 cut(s) 797
CseI GACGC 2 cut(s) 48, 249
Csp6I GTAC 1 cut(s) 423
CspI CGGWCCG 1 cut(s) 797
CviAII CATG 5 cut(s) 607, 683, 809, 821, 871
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 1 cut(s) 777
DpnI GATC 2 cut(s) 273, 708
DpnII GATC 2 cut(s) 271, 706
Eam1104I CTCTTC 3 cut(s) 345, 502, 822
EarI CTCTTC 3 cut(s) 345, 502, 822
EciI GGCGGA 1 cut(s) 576
Eco130I CCWWGG 2 cut(s) 771, 820
Eco31I GGTCTC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 1 cut(s) 621
Eco88I CYCGRG 2 cut(s) 80, 308
EcoT14I CCWWGG 2 cut(s) 771, 820
ErhI CCWWGG 2 cut(s) 771, 820
FaeI CATG 5 cut(s) 610, 686, 812, 824, 874
FaqI GGGAC 1 cut(s) 61
FatI CATG 5 cut(s) 606, 682, 808, 820, 870
FauI CCCGC 1 cut(s) 716
Fnu4HI GCNGC 2 cut(s) 590, 611
FokI GGATG 2 cut(s) 326, 462
Fsp4HI GCNGC 2 cut(s) 590, 611
FspBI CTAG 2 cut(s) 252, 365
FspI TGCGCA 1 cut(s) 605
GlaI GCGC 1 cut(s) 605
GluI GCNGC 2 cut(s) 590, 611
GsaI CCCAGC 1 cut(s) 836
HgaI GACGC 2 cut(s) 48, 249
HhaI GCGC 1 cut(s) 606
Hin1I GRCGYC 3 cut(s) 59, 260, 356
Hin1II CATG 5 cut(s) 610, 686, 812, 824, 874
Hin6I GCGC 1 cut(s) 604
HinP1I GCGC 1 cut(s) 604
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 3 cut(s) 137, 317, 583
HphI GGTGA 4 cut(s) 121, 641, 836, 955
Hpy166II GTNNAC 4 cut(s) 208, 425, 868, 946
Hpy188I TCNGA 4 cut(s) 64, 326, 376, 550
Hpy188III TCNNGA 2 cut(s) 82, 275
Hpy8I GTNNAC 4 cut(s) 208, 425, 868, 946
Hpy99I CGWCG 2 cut(s) 64, 361
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 4 cut(s) 158, 719, 801, 906
HpyCH4IV ACGT 3 cut(s) 74, 356, 864
HpyCH4V TGCA 8 cut(s) 300, 453, 514, 557, 661, 808, 874, 914
HpyF10VI GCNNNNNNNGC 1 cut(s) 610
HpyF3I CTNAG 1 cut(s) 777
HpySE526I ACGT 3 cut(s) 74, 356, 864
Hsp92I GRCGYC 3 cut(s) 59, 260, 356
Hsp92II CATG 5 cut(s) 610, 686, 812, 824, 874
HspAI GCGC 1 cut(s) 604
Kzo9I GATC 2 cut(s) 271, 706
LguI GCTCTTC 2 cut(s) 502, 822
LpnPI CCDG 5 cut(s) 260, 423, 554, 747, 818
LweI GCATC 3 cut(s) 211, 440, 662
MaeI CTAG 2 cut(s) 252, 365
MaeII ACGT 3 cut(s) 74, 356, 864
MaeIII GTNAC 5 cut(s) 109, 247, 629, 686, 791
MalI GATC 2 cut(s) 273, 708
MboI GATC 2 cut(s) 271, 706
MboII GAAGA 7 cut(s) 332, 489, 527, 767, 839, 929, 947
MflI RGATCY 2 cut(s) 271, 706
MluCI AATT 6 cut(s) 34, 130, 191, 429, 518, 663
MmeI TCCRAC 4 cut(s) 42, 211, 486, 684
MnlI CCTC 7 cut(s) 89, 317, 332, 440, 664, 669, 946
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 3 cut(s) 104, 695, 714
MspCI CTTAAG 1 cut(s) 694
MwoI GCNNNNNNNGC 1 cut(s) 610
NcoI CCATGG 1 cut(s) 820
NdeII GATC 2 cut(s) 271, 706
NlaIII CATG 5 cut(s) 610, 686, 812, 824, 874
NmuCI GTSAC 3 cut(s) 109, 247, 629
NsbI TGCGCA 1 cut(s) 605
NspI RCATGY 3 cut(s) 610, 812, 874
PaeI GCATGC 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 502, 822
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 137, 317, 583
PkrI GCNGC 2 cut(s) 591, 612
PshBI ATTAAT 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 832
PspPI GGNCC 1 cut(s) 797
PsuI RGATCY 2 cut(s) 271, 706
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
Rsr2I CGGWCCG 1 cut(s) 797
RsrII CGGWCCG 1 cut(s) 797
SapI GCTCTTC 2 cut(s) 502, 822
SaqAI TTAA 3 cut(s) 104, 695, 714
SatI GCNGC 2 cut(s) 590, 611
Sau3AI GATC 2 cut(s) 271, 706
Sau96I GGNCC 1 cut(s) 797
SetI ASST 9 cut(s) 77, 309, 359, 631, 680, 701, 834, 867, 900
SfaNI GCATC 3 cut(s) 211, 440, 662
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 797
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 3 cut(s) 42, 80, 694
SmoI CTYRAG 3 cut(s) 42, 80, 694
SpeI ACTAGT 1 cut(s) 251
SphI GCATGC 1 cut(s) 610
Sse9I AATT 6 cut(s) 34, 130, 191, 429, 518, 663
SsiI CCGC 4 cut(s) 587, 590, 610, 723
SspI AATATT 1 cut(s) 174
SspMI CTAG 2 cut(s) 252, 365
StyI CCWWGG 2 cut(s) 771, 820
TaaI ACNGT 4 cut(s) 158, 719, 801, 906
TaiI ACGT 3 cut(s) 77, 359, 867
TaqI TCGA 1 cut(s) 81
TasI AATT 6 cut(s) 34, 130, 191, 429, 518, 663
TatI WGTACW 1 cut(s) 422
TauI GCSGC 2 cut(s) 592, 613
TfiI GAWTC 3 cut(s) 137, 317, 583
Tru1I TTAA 3 cut(s) 104, 695, 714
Tru9I TTAA 3 cut(s) 104, 695, 714
TscAI CASTG 3 cut(s) 417, 911, 963
TseFI GTSAC 3 cut(s) 109, 247, 629
Tsp45I GTSAC 3 cut(s) 109, 247, 629
TspDTI ATGAA 4 cut(s) 87, 330, 423, 948
TspGWI ACGGA 3 cut(s) 17, 479, 810
TspRI CASTG 3 cut(s) 417, 911, 963
Vha464I CTTAAG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 797
VspI ATTAAT 1 cut(s) 104
XapI RAATTY 1 cut(s) 130
XceI RCATGY 3 cut(s) 610, 812, 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 238
XspI CTAG 2 cut(s) 252, 365
ZraI GACGTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.