MD10G1130200.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004183
Physical Location & Seq
Reverse (-)
21058055 .. 21059006
952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1130200.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGAAAGCATATTTCTAAGTGGCTTCTGTCGTGTTACAGACAGGGTTCAGTTAATCTATTTGGATTTTCATGACATTTCTGCAACTTCCCTTTCTTTAACACATGTTAGCTTGAGACGGTGCCCTCTTTTAACCAACCATGCAGTGACTAGTTTGGCGTCTAACAAGGATCTGGAAGTTTTGGATTTGAGAGATTGCAGAAAACTCGGGGATGAATCCCACCATGCCATAAGCACTCTTCCAAAAGTAAAGACATTGTTGCTAGATGGCTCTGATAGTAGTGATTTGGGATTATCGTATTTGAGACCAGTGGTTATAAGTTCACTAATTTCATTGTCTGTTAGAGGATGCAAGAAACTTACGGATAAATGTATCACTGCTCTATTTGATGGCTCTTCCAAGTTGGAGTTGCAAAAATTAGACTTGTCCAATCTTCCTAATCTCTCCGATAATGCAGTTTTATTACTGGCAAAGAGTAGGATTCCGCTACTTGAACTGCGCATGCGGCAATGCCCACTCATAGGTGACACTTCAGTAATGGCATTAGCATCTATGCAAGTTGACGAGGAGAGGTGGCATGGTAACTGCTTAAGGTTGTTGGATCTGTTTAATTGTGGGATTACCCCGCTTTCGTTTCGGTGGTTGAAGAAACCATATTTTCCAAGGCTGAGAATCAGATGGTTGGGAGTTACGGGTAGTGTAAATAGAGACATGGTAGATGCTTTGGCTCCAAGTGGACCGTTCTTGCATGTATCGTGCCATGGTGAGAAGCTGGGAGTTGAGCAAGGGGATACTGCCGATGATGTTTACATGCACGACTTTGATGAGATGGACGAGCTTGAACAGTGGCTTGCAGAAGATGATTATGTTGATGAAGATATGGTGAACGCAGAGGCAGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

302

Amino Acids

33.72

Weight (kDa)

5.07

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 16 - 233 1.1e-43 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 317
Acc16I TGCGCA 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 120
AciI CCGC 3 cut(s) 485, 505, 626
AclWI GGATC 2 cut(s) 177, 609
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 521
AflII CTTAAG 1 cut(s) 589
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 3 cut(s) 494, 646, 842
AhlI ACTAGT 1 cut(s) 149
AluBI AGCT 3 cut(s) 111, 772, 838
AluI AGCT 3 cut(s) 111, 772, 838
Alw26I GTCTC 3 cut(s) 109, 298, 702
AlwI GGATC 2 cut(s) 177, 609
Ama87I CYCGRG 1 cut(s) 206
AspLEI GCGC 1 cut(s) 501
AspS9I GGNCC 1 cut(s) 737
AsuHPI GGTGA 3 cut(s) 536, 776, 895
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 737
BaeGI GKGCMC 1 cut(s) 125
BanI GGYRCC 1 cut(s) 120
BccI CCATC 4 cut(s) 260, 383, 672, 823
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciVI GTATCC 1 cut(s) 784
BcoDI GTCTC 3 cut(s) 109, 298, 702
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 2 cut(s) 150, 263
BfrI CTTAAG 1 cut(s) 589
BfuI GTATCC 1 cut(s) 784
BisI GCNGC 1 cut(s) 506
BlsI GCNGC 1 cut(s) 507
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 737
BmiI GGNNCC 2 cut(s) 122, 729
BmsI GCATC 3 cut(s) 338, 557, 709
BpuEI CTTGAG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 158
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 662, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse1I ACTGG 2 cut(s) 308, 471
Bse3DI GCAATG 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 662, 760
BseGI GGATG 2 cut(s) 217, 353
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 1 cut(s) 659
BseNI ACTGG 2 cut(s) 308, 471
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 772
BshNI GGYRCC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 3 cut(s) 109, 298, 702
BsmBI CGTCTC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 298
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 169, 601
Bsp19I CCATGG 1 cut(s) 760
BspACI CCGC 3 cut(s) 485, 505, 626
BspCNI CTCAG 1 cut(s) 660
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 122, 729
BspPI GGATC 2 cut(s) 177, 609
BspQI GCTCTTC 1 cut(s) 400
BspT107I GGYRCC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 515
BsrI ACTGG 2 cut(s) 308, 471
BssECI CCNNGG 2 cut(s) 662, 760
BssMI GATC 2 cut(s) 169, 601
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 2 cut(s) 662, 760
Bst4CI ACNGT 3 cut(s) 120, 741, 846
Bst6I CTCTTC 2 cut(s) 243, 400
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 589
BstC8I GCNNGC 2 cut(s) 503, 852
BstDEI CTNAG 2 cut(s) 17, 668
BstDSI CCRYGG 1 cut(s) 760
BstF5I GGATG 2 cut(s) 217, 353
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 2 cut(s) 172, 604
BstMAI GTCTC 3 cut(s) 109, 298, 702
BstMBI GATC 2 cut(s) 169, 601
BstMWI GCNNNNNNNGC 1 cut(s) 505
BstNSI RCATGY 4 cut(s) 107, 505, 752, 814
BstSLI GKGCMC 1 cut(s) 125
BstX2I RGATCY 2 cut(s) 169, 601
BstYI RGATCY 2 cut(s) 169, 601
BsuI GTATCC 1 cut(s) 784
BtgI CCRYGG 1 cut(s) 760
BtsCI GGATG 2 cut(s) 217, 353
BtsI GCAGTG 3 cut(s) 150, 375, 903
BtsIMutI CAGTG 5 cut(s) 150, 315, 375, 851, 903
Cac8I GCNNGC 2 cut(s) 503, 852
CciI TCATGA 1 cut(s) 70
CfoI GCGC 1 cut(s) 501
Cfr13I GGNCC 1 cut(s) 737
CseI GACGC 1 cut(s) 147
CviJI RGCY 9 cut(s) 24, 111, 270, 393, 667, 728, 772, 838, 850
CviKI_1 RGCY 9 cut(s) 24, 111, 270, 393, 667, 728, 772, 838, 850
DdeI CTNAG 2 cut(s) 17, 668
DpnI GATC 2 cut(s) 171, 603
DpnII GATC 2 cut(s) 169, 601
Eam1104I CTCTTC 2 cut(s) 243, 400
EarI CTCTTC 2 cut(s) 243, 400
Eco130I CCWWGG 2 cut(s) 662, 760
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 662, 760
ErhI CCWWGG 2 cut(s) 662, 760
Esp3I CGTCTC 1 cut(s) 109
FauI CCCGC 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 506
FokI GGATG 2 cut(s) 224, 360
Fsp4HI GCNGC 1 cut(s) 506
FspBI CTAG 2 cut(s) 150, 263
FspI TGCGCA 1 cut(s) 500
GlaI GCGC 1 cut(s) 500
GluI GCNGC 1 cut(s) 506
GsaI CCCAGC 1 cut(s) 776
HgaI GACGC 1 cut(s) 147
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 158
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 3 cut(s) 215, 481, 672
HphI GGTGA 3 cut(s) 536, 776, 895
Hpy166II GTNNAC 5 cut(s) 323, 562, 737, 808, 886
Hpy188I TCNGA 3 cut(s) 274, 448, 677
Hpy188III TCNNGA 2 cut(s) 71, 173
Hpy8I GTNNAC 5 cut(s) 323, 562, 737, 808, 886
HpyCH4III ACNGT 3 cut(s) 120, 741, 846
HpyF10VI GCNNNNNNNGC 1 cut(s) 505
HpyF3I CTNAG 2 cut(s) 17, 668
Hsp92I GRCGYC 1 cut(s) 158
HspAI GCGC 1 cut(s) 499
Kzo9I GATC 2 cut(s) 169, 601
LguI GCTCTTC 1 cut(s) 400
LmnI GCTCC 1 cut(s) 733
LpnPI CCDG 5 cut(s) 28, 158, 321, 452, 758
LweI GCATC 3 cut(s) 338, 557, 709
MaeI CTAG 2 cut(s) 150, 263
MaeIII GTNAC 5 cut(s) 34, 145, 524, 581, 688
MalI GATC 2 cut(s) 171, 603
MboI GATC 2 cut(s) 169, 601
MboII GAAGA 6 cut(s) 230, 387, 425, 658, 869, 887
MflI RGATCY 2 cut(s) 169, 601
MhlI GDGCHC 1 cut(s) 125
MluCI AATT 3 cut(s) 327, 416, 610
MmeI TCCRAC 2 cut(s) 384, 579
MnlI CCTC 5 cut(s) 135, 338, 559, 564, 886
MseI TTAA 5 cut(s) 53, 98, 131, 590, 609
MspCI CTTAAG 1 cut(s) 589
MwoI GCNNNNNNNGC 1 cut(s) 505
NcoI CCATGG 1 cut(s) 760
NdeII GATC 2 cut(s) 169, 601
NlaIV GGNNCC 2 cut(s) 122, 729
NmuCI GTSAC 2 cut(s) 145, 524
NsbI TGCGCA 1 cut(s) 500
NspI RCATGY 4 cut(s) 107, 505, 752, 814
PaeI GCATGC 1 cut(s) 505
PagI TCATGA 1 cut(s) 70
PciI ACATGT 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 215, 481, 672
PkrI GCNGC 1 cut(s) 507
PscI ACATGT 1 cut(s) 103
PsiI TTATAA 1 cut(s) 317
PspFI CCCAGC 1 cut(s) 772
PspN4I GGNNCC 2 cut(s) 122, 729
PspPI GGNCC 1 cut(s) 737
PsuI RGATCY 2 cut(s) 169, 601
SapI GCTCTTC 1 cut(s) 400
SaqAI TTAA 5 cut(s) 53, 98, 131, 590, 609
SatI GCNGC 1 cut(s) 506
Sau3AI GATC 2 cut(s) 169, 601
Sau96I GGNCC 1 cut(s) 737
SduI GDGCHC 1 cut(s) 125
SetI ASST 6 cut(s) 113, 526, 575, 596, 774, 840
SfaNI GCATC 3 cut(s) 338, 557, 709
SinI GGWCC 1 cut(s) 737
SmlI CTYRAG 2 cut(s) 112, 589
SmoI CTYRAG 2 cut(s) 112, 589
SpeI ACTAGT 1 cut(s) 149
SphI GCATGC 1 cut(s) 505
Sse9I AATT 3 cut(s) 327, 416, 610
SsiI CCGC 3 cut(s) 485, 505, 626
SspMI CTAG 2 cut(s) 150, 263
StyI CCWWGG 2 cut(s) 662, 760
TaaI ACNGT 3 cut(s) 120, 741, 846
TasI AATT 3 cut(s) 327, 416, 610
TauI GCSGC 1 cut(s) 508
TfiI GAWTC 3 cut(s) 215, 481, 672
Tru1I TTAA 5 cut(s) 53, 98, 131, 590, 609
Tru9I TTAA 5 cut(s) 53, 98, 131, 590, 609
TscAI CASTG 5 cut(s) 150, 315, 382, 851, 903
TseFI GTSAC 2 cut(s) 145, 524
Tsp45I GTSAC 2 cut(s) 145, 524
TspDTI ATGAA 4 cut(s) 59, 228, 321, 888
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 5 cut(s) 150, 315, 382, 851, 903
Vha464I CTTAAG 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 737
XceI RCATGY 4 cut(s) 107, 505, 752, 814
XspI CTAG 2 cut(s) 150, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.