Rw6G020270

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004183
Physical Location & Seq
Reverse (-)
40547595 .. 40550749
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G020270.1

Sequence Viewer

Length: 1437 bp
ATGTTTGATGTCTCGTTTTCACTGCGTCATGACTTTGCTCGAGTTTGGGCTTTGGCCTCTGAGAAACTCACTTCTCTTGAGATTGGCTACGTTTCATCTGTAATGGTGACTGAACTACTTAGCCCAAATTTCAAATTACCGCAGTCAGCAAATCGTATTCAGCCTTCCATATTGCCTAATATTCATAAATTGTCCCTTTCCGTCGACTACATAACTGATGCCATGGTTGGTACTATATCTAAAGGTCTCACCTCCTTGACCCATTTGGATCTTCGGGATGCACCCCTCATTGAACCAAGAGTTACATTTGACCTTACCAACTCTGGCCTTCAACTGATAAATCAGCATGGAAAGTTGAAGCATTTATCATTGGTTCGGAGCCAGGAGTTTCTCTTCACATATTTCAAACGAGTAAATGATCTGGGGCTCCTTTTATTAGCCGACAAGTGTGAAAAGATGGAAAGTATATTTCTCAGTGGCTTCTGTCGTGTTACAGACACAGGTTTCAAAACAATCTTGCATTCTTGCTTTACGCTCTACAAGCTTAGGGTGTCTCATGGGACTCAGTTAACTGATCTGGTTTTTCATGACATCTCTGCAACTTCCCTTTCTTTAACACACGTTAGCTTGAGACGGTGTACACTTTTAACCAACCATGCAGTCACTAATTTGGCTTCTAACAAGGATCTAAAAGTTCTTGATTTGAGAGATTGCAGAAACCTTGGGAATGAAGCCCTCCGAGCCATAGGCAATCTTCATAATTTGAAGACCTTATTATTGGATGGCTCCGATATCAGTGATCTGGGATTATCATACTTAAGACCAAGGGTTATAAGTTCACTAATTACATTGTCTGTTAGAGGCTGCAAGAAACTGACTGATAAATGCATCTCTGCTCTATTTGATGGCTCTTCTAAATTGGCGTTGCAAGAATTGGACCTATCAAATCTTCCGAATCTCTCTGACAATGGAGTTTTATTACTTGCAAGAAGTAGGATTCCGTTATTGGAACTACGAATGCGACAATGCCCACTCATAGGTGACACATCTGTAGTGGTATTAGCTTCAATGCTGGTAGACGAGGAAAGGTGGCATGGTAATTGCTTGCGGTTGTTGGATCTGTTTAACTGTGGTGGCATTACTGTGGTTGCATTTCGGTGGCTGAAGAAACCATATTTTCCAAGGCTAAGGTGGTTGGGGGTTACAGGTTGTGTAAATAGAGATACAGTAGATGCTTTAGCTCGTAGTAGACCGTTCTTGCATGTGTCATGCCATGGTGAGGAGCTGGGGGCAGAGCAGTGGGATACCGGGGACGACTTTCATATGCATGACTATGATGAGATGGATGAGCTTGAACAGTGGCTTCAAGAAGGTGACTATGAGAGTGGCGATGAAGAAATGGTGGAGGCAGAAAACGTTGCAGAAGCGGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

478

Amino Acids

53.66

Weight (kDa)

5.75

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 48 - 431 2.1e-94 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccI GTMKAC 3 cut(s) 204, 1077, 1249
AciI CCGC 3 cut(s) 140, 1108, 1427
AclI AACGTT 1 cut(s) 1416
AclWI GGATC 3 cut(s) 276, 693, 1125
AcsI RAATTY 1 cut(s) 127
AcuI CTGAAG 1 cut(s) 1184
AfaI GTAC 2 cut(s) 232, 640
AfiI CCNNNNNNNGG 2 cut(s) 1037, 1279
AflII CTTAAG 1 cut(s) 817
AflIII ACRYGT 1 cut(s) 619
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 6 cut(s) 544, 627, 1064, 1241, 1285, 1351
AluI AGCT 6 cut(s) 544, 627, 1064, 1241, 1285, 1351
Alw26I GTCTC 4 cut(s) 16, 251, 558, 625
AlwI GGATC 3 cut(s) 276, 693, 1125
Ama87I CYCGRG 1 cut(s) 39
AoxI GGCC 2 cut(s) 54, 325
ApeKI GCWGC 1 cut(s) 864
ApoI RAATTY 1 cut(s) 127
AspS9I GGNCC 1 cut(s) 937
AsuC2I CCSGG 1 cut(s) 1309
AsuHPI GGTGA 5 cut(s) 118, 241, 1052, 1289, 1385
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 1 cut(s) 937
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 148, 180
BbsI GAAGAC 1 cut(s) 773
BbvI GCAGC 1 cut(s) 851
BccI CCATC 4 cut(s) 451, 776, 899, 1336
BciT130I CCWGG 1 cut(s) 383
BciVI GTATCC 1 cut(s) 1297
BcnI CCSGG 1 cut(s) 1309
BcoDI GTCTC 4 cut(s) 16, 251, 558, 625
BfmI CTRYAG 1 cut(s) 1050
BfrI CTTAAG 1 cut(s) 817
BfuI GTATCC 1 cut(s) 1297
BisI GCNGC 1 cut(s) 865
BlsI GCNGC 1 cut(s) 866
Bme1390I CCNGG 2 cut(s) 383, 1309
Bme18I GGWCC 1 cut(s) 937
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 937
BmiI GGNNCC 3 cut(s) 380, 428, 787
BmrFI CCNGG 2 cut(s) 383, 1309
BmsI GCATC 4 cut(s) 208, 268, 897, 1222
BpiI GAAGAC 1 cut(s) 773
Bpu10I CCTNAGC 2 cut(s) 545, 1187
BpuEI CTTGAG 2 cut(s) 98, 649
BpuMI CCSGG 1 cut(s) 1309
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 6 cut(s) 222, 721, 824, 1181, 1273, 1308
Bsc4I CCNNNNNNNGG 2 cut(s) 1037, 1279
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 6 cut(s) 222, 721, 824, 1181, 1273, 1308
BseGI GGATG 3 cut(s) 283, 787, 1351
BseLI CCNNNNNNNGG 2 cut(s) 1037, 1279
BseMII CTCAG 3 cut(s) 51, 487, 578
BseRI GAGGAG 1 cut(s) 1295
BseXI GCAGC 1 cut(s) 851
BseYI CCCAGC 1 cut(s) 1285
BshFI GGCC 2 cut(s) 56, 327
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 1308
BslFI GGGAC 3 cut(s) 178, 574, 1325
BslI CCNNNNNNNGG 2 cut(s) 1037, 1279
BsmAI GTCTC 4 cut(s) 16, 251, 558, 625
BsmBI CGTCTC 1 cut(s) 625
BsmFI GGGAC 3 cut(s) 178, 574, 1325
BsmI GAATGC 2 cut(s) 520, 1023
BsnI GGCC 2 cut(s) 56, 327
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 638
Bsp143I GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
Bsp19I CCATGG 2 cut(s) 222, 1273
BspACI CCGC 3 cut(s) 140, 1108, 1427
BspANI GGCC 2 cut(s) 56, 327
BspCNI CTCAG 3 cut(s) 52, 486, 577
BspHI TCATGA 2 cut(s) 28, 586
BspLI GGNNCC 3 cut(s) 380, 428, 787
BspPI GGATC 3 cut(s) 276, 693, 1125
BspQI GCTCTTC 1 cut(s) 916
BspTI CTTAAG 1 cut(s) 817
BspTNI GGTCTC 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 638
BssECI CCNNGG 6 cut(s) 222, 721, 824, 1181, 1273, 1308
BssMI GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
BssT1I CCWWGG 5 cut(s) 222, 721, 824, 1181, 1273
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 6 cut(s) 636, 1130, 1144, 1228, 1254, 1359
Bst6I CTCTTC 2 cut(s) 398, 916
BstAFI CTTAAG 1 cut(s) 817
BstAUI TGTACA 1 cut(s) 638
BstC8I GCNNGC 1 cut(s) 1106
BstDEI CTNAG 6 cut(s) 60, 119, 473, 545, 564, 1187
BstDSI CCRYGG 2 cut(s) 222, 1273
BstF5I GGATG 3 cut(s) 283, 787, 1351
BstKTI GATC 6 cut(s) 271, 421, 577, 688, 802, 1120
BstMAI GTCTC 4 cut(s) 16, 251, 558, 625
BstMBI GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 541, 740
BstNI CCWGG 1 cut(s) 383
BstNSI RCATGY 1 cut(s) 1265
BstSCI CCNGG 2 cut(s) 381, 1307
BstSFI CTRYAG 1 cut(s) 1050
BstV1I GCAGC 1 cut(s) 851
BstV2I GAAGAC 1 cut(s) 773
BstX2I RGATCY 3 cut(s) 268, 685, 1117
BstYI RGATCY 3 cut(s) 268, 685, 1117
BsuI GTATCC 1 cut(s) 1297
BsuRI GGCC 2 cut(s) 56, 327
BtgI CCRYGG 2 cut(s) 222, 1273
BtgZI GCGATG 1 cut(s) 1404
BtsCI GGATG 3 cut(s) 283, 787, 1351
BtsI GCAGTG 2 cut(s) 20, 1304
BtsIMutI CAGTG 5 cut(s) 20, 481, 802, 1304, 1364
Cac8I GCNNGC 1 cut(s) 1106
CciI TCATGA 2 cut(s) 28, 586
Cfr13I GGNCC 1 cut(s) 937
CseI GACGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 231, 639
CviQI GTAC 2 cut(s) 231, 639
DdeI CTNAG 6 cut(s) 60, 119, 473, 545, 564, 1187
DpnI GATC 6 cut(s) 270, 420, 576, 687, 801, 1119
DpnII GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
Eam1104I CTCTTC 2 cut(s) 398, 916
EarI CTCTTC 2 cut(s) 398, 916
Eco130I CCWWGG 5 cut(s) 222, 721, 824, 1181, 1273
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 251
Eco32I GATATC 1 cut(s) 793
Eco47I GGWCC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 1184
Eco88I CYCGRG 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 381
EcoRV GATATC 1 cut(s) 793
EcoT14I CCWWGG 5 cut(s) 222, 721, 824, 1181, 1273
EcoT22I ATGCAT 2 cut(s) 890, 1329
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 5 cut(s) 222, 721, 824, 1181, 1273
Esp3I CGTCTC 1 cut(s) 625
FaqI GGGAC 3 cut(s) 178, 574, 1325
FauNDI CATATG 1 cut(s) 1323
FblI GTMKAC 3 cut(s) 204, 1077, 1249
Fnu4HI GCNGC 1 cut(s) 865
FokI GGATG 3 cut(s) 290, 794, 1358
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 1 cut(s) 865
GluI GCNGC 1 cut(s) 865
GsaI CCCAGC 1 cut(s) 1289
HaeIII GGCC 2 cut(s) 56, 327
HapII CCGG 1 cut(s) 1308
HgaI GACGC 1 cut(s) 14
HincII GTYRAC 2 cut(s) 205, 570
HindII GTYRAC 2 cut(s) 205, 570
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 3 cut(s) 562, 955, 997
HpaI GTTAAC 1 cut(s) 570
HpaII CCGG 1 cut(s) 1308
HphI GGTGA 5 cut(s) 118, 241, 1052, 1289, 1385
Hpy166II GTNNAC 7 cut(s) 205, 570, 639, 641, 839, 1078, 1250
Hpy188I TCNGA 6 cut(s) 61, 378, 740, 790, 954, 964
Hpy188III TCNNGA 6 cut(s) 29, 77, 275, 587, 698, 1367
Hpy8I GTNNAC 7 cut(s) 205, 570, 639, 641, 839, 1078, 1250
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 174, 338, 1364
HpyCH4III ACNGT 6 cut(s) 636, 1130, 1144, 1228, 1254, 1359
HpyCH4IV ACGT 3 cut(s) 90, 621, 1416
HpyF10VI GCNNNNNNNGC 2 cut(s) 541, 740
HpyF3I CTNAG 6 cut(s) 60, 119, 473, 545, 564, 1187
HpySE526I ACGT 3 cut(s) 90, 621, 1416
KspAI GTTAAC 1 cut(s) 570
Kzo9I GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
LguI GCTCTTC 1 cut(s) 916
LmnI GCTCC 4 cut(s) 378, 432, 791, 1282
Lsp1109I GCAGC 1 cut(s) 851
LweI GCATC 4 cut(s) 208, 268, 897, 1222
MaeII ACGT 3 cut(s) 90, 621, 1416
MaeIII GTNAC 7 cut(s) 106, 301, 490, 661, 1040, 1201, 1373
MalI GATC 6 cut(s) 270, 420, 576, 687, 801, 1119
MboI GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
MboII GAAGA 8 cut(s) 263, 385, 746, 778, 903, 941, 1177, 1406
MflI RGATCY 3 cut(s) 268, 685, 1117
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 9 cut(s) 127, 134, 188, 667, 760, 843, 917, 932, 1099
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 1 cut(s) 1095
MnlI CCTC 8 cut(s) 67, 262, 296, 746, 854, 1075, 1273, 1399
Mph1103I ATGCAT 2 cut(s) 890, 1329
MseI TTAA 5 cut(s) 569, 614, 647, 818, 1125
MslI CAYNNNNRTG 4 cut(s) 1142, 1156, 1326, 1332
MspCI CTTAAG 1 cut(s) 817
MspI CCGG 1 cut(s) 1308
MspR9I CCNGG 2 cut(s) 383, 1309
Mva1269I GAATGC 2 cut(s) 520, 1023
MvaI CCWGG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 541, 740
NciI CCSGG 1 cut(s) 1309
NcoI CCATGG 2 cut(s) 222, 1273
NdeI CATATG 1 cut(s) 1323
NdeII GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
NlaIV GGNNCC 3 cut(s) 380, 428, 787
NmuCI GTSAC 4 cut(s) 106, 661, 1040, 1373
NsiI ATGCAT 2 cut(s) 890, 1329
NspI RCATGY 1 cut(s) 1265
PaeR7I CTCGAG 1 cut(s) 39
PagI TCATGA 2 cut(s) 28, 586
PciSI GCTCTTC 1 cut(s) 916
PctI GAATGC 2 cut(s) 520, 1023
PfeI GAWTC 2 cut(s) 955, 997
PkrI GCNGC 1 cut(s) 866
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PsiI TTATAA 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 1416
Psp6I CCWGG 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 1285
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 380, 428, 787
PspPI GGNCC 1 cut(s) 937
PspXI VCTCGAGB 1 cut(s) 39
PsuI RGATCY 3 cut(s) 268, 685, 1117
RsaI GTAC 2 cut(s) 232, 640
RsaNI GTAC 2 cut(s) 231, 639
RseI CAYNNNNRTG 4 cut(s) 1142, 1156, 1326, 1332
SalI GTCGAC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 916
SaqAI TTAA 5 cut(s) 569, 614, 647, 818, 1125
SatI GCNGC 1 cut(s) 865
Sau3AI GATC 6 cut(s) 268, 418, 574, 685, 799, 1117
Sau96I GGNCC 1 cut(s) 937
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 2 cut(s) 383, 1309
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 4 cut(s) 208, 268, 897, 1222
SfcI CTRYAG 1 cut(s) 1050
Sfr274I CTCGAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 937
SlaI CTCGAG 1 cut(s) 39
SmiMI CAYNNNNRTG 4 cut(s) 1142, 1156, 1326, 1332
SmlI CTYRAG 4 cut(s) 39, 77, 628, 817
SmoI CTYRAG 4 cut(s) 39, 77, 628, 817
Sse9I AATT 9 cut(s) 127, 134, 188, 667, 760, 843, 917, 932, 1099
SsiI CCGC 3 cut(s) 140, 1108, 1427
SspI AATATT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 381, 1307
StyI CCWWGG 5 cut(s) 222, 721, 824, 1181, 1273
TaaI ACNGT 6 cut(s) 636, 1130, 1144, 1228, 1254, 1359
TaiI ACGT 3 cut(s) 93, 624, 1419
TaqI TCGA 2 cut(s) 40, 204
TasI AATT 9 cut(s) 127, 134, 188, 667, 760, 843, 917, 932, 1099
TatI WGTACW 1 cut(s) 638
TfiI GAWTC 2 cut(s) 955, 997
Tru1I TTAA 5 cut(s) 569, 614, 647, 818, 1125
Tru9I TTAA 5 cut(s) 569, 614, 647, 818, 1125
TscAI CASTG 5 cut(s) 27, 481, 802, 1304, 1364
TseFI GTSAC 4 cut(s) 106, 661, 1040, 1373
TseI GCWGC 1 cut(s) 864
Tsp45I GTSAC 4 cut(s) 106, 661, 1040, 1373
TspDTI ATGAA 7 cut(s) 84, 173, 575, 744, 746, 1310, 1407
TspGWI ACGGA 2 cut(s) 190, 990
TspRI CASTG 5 cut(s) 27, 481, 802, 1304, 1364
Vha464I CTTAAG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 1 cut(s) 127
XceI RCATGY 1 cut(s) 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 1188
XhoI CTCGAG 1 cut(s) 39
XmiI GTMKAC 3 cut(s) 204, 1077, 1249
Zsp2I ATGCAT 2 cut(s) 890, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.