Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004010

Physical Location & Seq

Genomic Coordinates Forward (+)

26523169 .. 26523979

Gene Structure 811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1137000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGTCCTCCCAAGATGTTGAGTTCAGGGGACCACAAATACGATCTCAGTTTAATTCTGCTCCAACAGCGACCCAGGGTGATGCTGTTACTCCAACATCCTCCCAGAATCTTGGGTTCAGGGGATTAAGTGTGAATGTGAACCAGCAAAATTTCATTTCTCAAGATGGCAAGTCAATTAGGCCTCCAGTGCCTCCATCTAGTTCTGATTCCCAGCCCACACAGAGTGTTGCTGCCCAAGGCTTTCCCAGTGAAGGTTCAGTGGGTGGTCCTCAACCACAAAATTCAAACATGTCTAATGATTGGGTTGGTGGAAGGGCAAGTGGAGCAACGACCGAGATACCCTCGCAGATTGTAAAAAAGGGGATCACTCCTTCTGCAACACAAGATGTATTTGGGCAGGCAACATCAGGACCAACAACTTCCCTACCACCTAGGCCACAGGCAGGTTTTGGGTTCAGACCACCGGCTAAGGATTCCAAACCATCAAATATATCTAGGAATGGCTTTGCTCCTTACTCATCTTTTGGAGTCGATGTGTTTTCTGCAACCCCATCTCAACCAAAGCAGAATTTTCCTCTTGGCAGTATACCCGTCTCATCAACCATTGTGCCAGTATCTGCTTGGACCCAATCTTCAGTTACAGTTATGCGCCTATATAGTATGACCACAAACATAACTTTTGGAAGCTATAAGTTCCCCCACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

236

Amino Acids

24.66

Weight (kDa)

9.81

Isoelectric Point (pI)

60.14

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21630 AT1G21630 AT1G21630 AT1G21630
fragaria_vesca	FvH4_2g16750 FvH4_2g16750 FvH4_2g16750
malus_domestica	MD04G1035400.v1.1 MD05G1135500.v1.1 MD05G1137000.v1.1 MD10G1140800.v1.1
prunus_persica	Prupe.8G179600_v2.0.a1 Prupe.8G179600_v2.0.a1 Prupe.8G179600_v2.0.a1
pyrus_communis	pycom03g08400 pycom05g13210 pycom05g13310 pycom10g11940 pycom10g11970
rosa_chinensis	RchiOBHm_Chr6g0280871
rosa_laevigata	RLG00000013024
rosa_multiflora	Rmu_co8142976.1_g000001 Rmu_co8285335.1_g000001 Rmu_sc0004772.1_g000002 Rmu_sc0026236.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187540
rosa_rugosa	Rorug06G0135900
rosa_samantha	Rh6AG248700 Rh6BG250400 Rh6BG250600 Rh6CG251400 Rh6DG243100
rosa_wichuraiana	Rw6G021500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	434
AccI	GTMKAC	1 cut(s)	586
AclWI	GGATC	1 cut(s)	371
AcsI	RAATTY	3 cut(s)	148, 280, 568
AcuI	CTGAAG	1 cut(s)	618
AdeI	CACNNNGTG	1 cut(s)	224
AfiI	CCNNNNNNNGG	2 cut(s)	251, 443
AflIII	ACRYGT	1 cut(s)	288
AgsI	TTSAA	1 cut(s)	285
AjnI	CCWGG	1 cut(s)	72
AluBI	AGCT	1 cut(s)	687
AluI	AGCT	1 cut(s)	687
Alw26I	GTCTC	1 cut(s)	598
AlwI	GGATC	1 cut(s)	371
AlwNI	CAGNNNCTG	1 cut(s)	617
AoxI	GGCC	2 cut(s)	179, 434
ApeKI	GCWGC	1 cut(s)	230
ApoI	RAATTY	3 cut(s)	148, 280, 568
AspA2I	CCTAGG	1 cut(s)	431
AspLEI	GCGC	1 cut(s)	651
AspS9I	GGNCC	4 cut(s)	29, 266, 410, 624
AsuHPI	GGTGA	1 cut(s)	89
AvaII	GGWCC	4 cut(s)	29, 266, 410, 624
AvrII	CCTAGG	1 cut(s)	431
BbvI	GCAGC	1 cut(s)	217
BccI	CCATC	4 cut(s)	158, 202, 490, 559
BciT130I	CCWGG	1 cut(s)	74
BcoDI	GTCTC	1 cut(s)	598
BfaI	CTAG	3 cut(s)	198, 432, 495
BfuAI	ACCTGC	1 cut(s)	434
BisI	GCNGC	1 cut(s)	231
BlnI	CCTAGG	1 cut(s)	431
BlsI	GCNGC	1 cut(s)	232
Bme1390I	CCNGG	1 cut(s)	74
Bme18I	GGWCC	4 cut(s)	29, 266, 410, 624
BmgT120I	GGNCC	4 cut(s)	29, 266, 410, 624
BmiI	GGNNCC	2 cut(s)	30, 626
BmrFI	CCNGG	1 cut(s)	74
BmrI	ACTGGG	1 cut(s)	240
BmsI	GCATC	1 cut(s)	70
BmuI	ACTGGG	1 cut(s)	240
BplI	GAGNNNNNCTC	2 cut(s)	326, 358
BpmI	CTGGAG	1 cut(s)	168
Bpu10I	CCTNAGC	1 cut(s)	468
BpuEI	CTTGAG	1 cut(s)	144
BsaJI	CCNNGG	4 cut(s)	72, 73, 235, 431
BsaXI	ACNNNNNCTCC	2 cut(s)	519, 549
Bsc4I	CCNNNNNNNGG	2 cut(s)	251, 443
Bse118I	RCCGGY	1 cut(s)	463
Bse1I	ACTGG	3 cut(s)	185, 246, 611
BseBI	CCWGG	1 cut(s)	74
BseDI	CCNNGG	4 cut(s)	72, 73, 235, 431
BseGI	GGATG	1 cut(s)	95
BseLI	CCNNNNNNNGG	2 cut(s)	251, 443
BseMII	CTCAG	1 cut(s)	59
BseNI	ACTGG	3 cut(s)	185, 246, 611
BseXI	GCAGC	1 cut(s)	217
BseYI	CCCAGC	1 cut(s)	210
Bsh1285I	CGRYCG	1 cut(s)	333
BshFI	GGCC	2 cut(s)	181, 436
BsiEI	CGRYCG	1 cut(s)	333
BsiSI	CCGG	1 cut(s)	464
BslFI	GGGAC	1 cut(s)	42
BslI	CCNNNNNNNGG	2 cut(s)	251, 443
BsmAI	GTCTC	1 cut(s)	598
BsmBI	CGTCTC	1 cut(s)	598
BsmFI	GGGAC	1 cut(s)	42
BsnI	GGCC	2 cut(s)	181, 436
Bsp143I	GATC	2 cut(s)	41, 363
BspANI	GGCC	2 cut(s)	181, 436
BspCNI	CTCAG	1 cut(s)	58
BspLI	GGNNCC	2 cut(s)	30, 626
BspMI	ACCTGC	1 cut(s)	434
BspPI	GGATC	1 cut(s)	371
BsrFI	RCCGGY	1 cut(s)	463
BsrI	ACTGG	3 cut(s)	185, 246, 611
BssAI	RCCGGY	1 cut(s)	463
BssECI	CCNNGG	4 cut(s)	72, 73, 235, 431
BssMI	GATC	2 cut(s)	41, 363
BssNAI	GTATAC	1 cut(s)	587
BssT1I	CCWWGG	2 cut(s)	235, 431
Bst1107I	GTATAC	1 cut(s)	587
Bst2UI	CCWGG	1 cut(s)	74
Bst4CI	ACNGT	1 cut(s)	643
BstC8I	GCNNGC	1 cut(s)	399
BstDEI	CTNAG	2 cut(s)	45, 468
BstF5I	GGATG	1 cut(s)	95
BstHHI	GCGC	1 cut(s)	651
BstKTI	GATC	2 cut(s)	44, 366
BstMAI	GTCTC	1 cut(s)	598
BstMBI	GATC	2 cut(s)	41, 363
BstMCI	CGRYCG	1 cut(s)	333
BstMWI	GCNNNNNNNGC	3 cut(s)	65, 187, 323
BstNI	CCWGG	1 cut(s)	74
BstNSI	RCATGY	1 cut(s)	292
BstSCI	CCNGG	1 cut(s)	72
BstV1I	GCAGC	1 cut(s)	217
BstXI	CCANNNNNNTGG	1 cut(s)	110
BstZ17I	GTATAC	1 cut(s)	587
BsuRI	GGCC	2 cut(s)	181, 436
BtsCI	GGATG	1 cut(s)	95
BtsIMutI	CAGTG	3 cut(s)	192, 253, 264
BveI	ACCTGC	1 cut(s)	434
Cac8I	GCNNGC	1 cut(s)	399
CaiI	CAGNNNCTG	1 cut(s)	617
CfoI	GCGC	1 cut(s)	651
Cfr10I	RCCGGY	1 cut(s)	463
Cfr13I	GGNCC	4 cut(s)	29, 266, 410, 624
CviAII	CATG	1 cut(s)	289
CviJI	RGCY	7 cut(s)	181, 214, 240, 436, 467, 504, 687
CviKI_1	RGCY	7 cut(s)	181, 214, 240, 436, 467, 504, 687
DdeI	CTNAG	2 cut(s)	45, 468
DpnI	GATC	2 cut(s)	43, 365
DpnII	GATC	2 cut(s)	41, 363
DraIII	CACNNNGTG	1 cut(s)	224
Eco130I	CCWWGG	2 cut(s)	235, 431
Eco147I	AGGCCT	1 cut(s)	181
Eco47I	GGWCC	4 cut(s)	29, 266, 410, 624
Eco57I	CTGAAG	1 cut(s)	618
EcoRII	CCWGG	1 cut(s)	72
EcoT14I	CCWWGG	2 cut(s)	235, 431
ErhI	CCWWGG	2 cut(s)	235, 431
Esp3I	CGTCTC	1 cut(s)	598
FaeI	CATG	1 cut(s)	292
FaiI	YATR	9 cut(s)	290, 491, 587, 647, 655, 657, 662, 674, 690
FaqI	GGGAC	1 cut(s)	42
FatI	CATG	1 cut(s)	288
FblI	GTMKAC	1 cut(s)	586
Fnu4HI	GCNGC	1 cut(s)	231
FokI	GGATG	1 cut(s)	82
Fsp4HI	GCNGC	1 cut(s)	231
FspBI	CTAG	3 cut(s)	198, 432, 495
GlaI	GCGC	1 cut(s)	650
GluI	GCNGC	1 cut(s)	231
GsaI	CCCAGC	1 cut(s)	214
GsuI	CTGGAG	1 cut(s)	168
HaeIII	GGCC	2 cut(s)	181, 436
HapII	CCGG	1 cut(s)	464
HhaI	GCGC	1 cut(s)	651
Hin1II	CATG	1 cut(s)	292
Hin6I	GCGC	1 cut(s)	649
HinP1I	GCGC	1 cut(s)	649
HinfI	GANTC	4 cut(s)	106, 206, 473, 528
HpaII	CCGG	1 cut(s)	464
HphI	GGTGA	1 cut(s)	89
Hpy166II	GTNNAC	2 cut(s)	139, 587
Hpy188I	TCNGA	2 cut(s)	205, 458
Hpy188III	TCNNGA	2 cut(s)	161, 408
Hpy8I	GTNNAC	2 cut(s)	139, 587
HpyAV	CCTTC	3 cut(s)	245, 306, 381
HpyCH4III	ACNGT	1 cut(s)	643
HpyCH4V	TGCA	2 cut(s)	377, 545
HpyF10VI	GCNNNNNNNGC	3 cut(s)	65, 187, 323
HpyF3I	CTNAG	2 cut(s)	45, 468
Hsp92II	CATG	1 cut(s)	292
HspAI	GCGC	1 cut(s)	649
Kzo9I	GATC	2 cut(s)	41, 363
LmnI	GCTCC	3 cut(s)	64, 323, 514
Lsp1109I	GCAGC	1 cut(s)	217
LweI	GCATC	1 cut(s)	70
MaeI	CTAG	3 cut(s)	198, 432, 495
MaeIII	GTNAC	2 cut(s)	85, 637
MalI	GATC	2 cut(s)	43, 365
MboI	GATC	2 cut(s)	41, 363
MboII	GAAGA	1 cut(s)	624
MluCI	AATT	5 cut(s)	52, 148, 174, 280, 568
MlyI	GAGTC	1 cut(s)	537
MmeI	TCCRAC	2 cut(s)	86, 116
MnlI	CCTC	7 cut(s)	16, 109, 192, 201, 279, 352, 585
MseI	TTAA	3 cut(s)	51, 125, 706
MspI	CCGG	1 cut(s)	464
MspR9I	CCNGG	1 cut(s)	74
MvaI	CCWGG	1 cut(s)	74
MwoI	GCNNNNNNNGC	3 cut(s)	65, 187, 323
NdeII	GATC	2 cut(s)	41, 363
NlaIII	CATG	1 cut(s)	292
NlaIV	GGNNCC	2 cut(s)	30, 626
NspI	RCATGY	1 cut(s)	292
PasI	CCCWGGG	1 cut(s)	73
PceI	AGGCCT	1 cut(s)	181
PciI	ACATGT	1 cut(s)	288
PfeI	GAWTC	3 cut(s)	106, 206, 473
PkrI	GCNGC	1 cut(s)	232
PleI	GAGTC	1 cut(s)	536
PpsI	GAGTC	1 cut(s)	536
PscI	ACATGT	1 cut(s)	288
Psp6I	CCWGG	1 cut(s)	72
PspFI	CCCAGC	1 cut(s)	210
PspGI	CCWGG	1 cut(s)	72
PspN4I	GGNNCC	2 cut(s)	30, 626
PspPI	GGNCC	4 cut(s)	29, 266, 410, 624
PstNI	CAGNNNCTG	1 cut(s)	617
SaqAI	TTAA	3 cut(s)	51, 125, 706
SatI	GCNGC	1 cut(s)	231
Sau3AI	GATC	2 cut(s)	41, 363
Sau96I	GGNCC	4 cut(s)	29, 266, 410, 624
SchI	GAGTC	1 cut(s)	537
ScrFI	CCNGG	1 cut(s)	74
SetI	ASST	4 cut(s)	256, 433, 448, 689
SfaNI	GCATC	1 cut(s)	70
SinI	GGWCC	4 cut(s)	29, 266, 410, 624
SmlI	CTYRAG	1 cut(s)	159
SmoI	CTYRAG	1 cut(s)	159
Sse9I	AATT	5 cut(s)	52, 148, 174, 280, 568
SseBI	AGGCCT	1 cut(s)	181
SspMI	CTAG	3 cut(s)	198, 432, 495
StuI	AGGCCT	1 cut(s)	181
StyD4I	CCNGG	1 cut(s)	72
StyI	CCWWGG	2 cut(s)	235, 431
TaaI	ACNGT	1 cut(s)	643
TaqI	TCGA	1 cut(s)	531
TaqII	GACCGA	1 cut(s)	347
TasI	AATT	5 cut(s)	52, 148, 174, 280, 568
TfiI	GAWTC	3 cut(s)	106, 206, 473
Tru1I	TTAA	3 cut(s)	51, 125, 706
Tru9I	TTAA	3 cut(s)	51, 125, 706
TscAI	CASTG	3 cut(s)	192, 253, 264
TseI	GCWGC	1 cut(s)	230
TspDTI	ATGAA	1 cut(s)	142
TspRI	CASTG	3 cut(s)	192, 253, 264
VpaK11BI	GGWCC	4 cut(s)	29, 266, 410, 624
XapI	RAATTY	3 cut(s)	148, 280, 568
XceI	RCATGY	1 cut(s)	292
XcmI	CCANNNNNNNNNTGG	1 cut(s)	618
XmaJI	CCTAGG	1 cut(s)	431
XmiI	GTMKAC	1 cut(s)	586
XspI	CTAG	3 cut(s)	198, 432, 495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.