Rh6BG250600

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004010
Physical Location & Seq
Forward (+)
46243585 .. 46253070
9486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG250600.1

Sequence Viewer

Length: 2394 bp
ATGAAGAATTTTCTCTGCGATTTTCGCTTCCTCCTACTTCTCGCAGCTGCCGCCTTCATCTATATCCAGGTTCGGCTTTTCGCGACACAATCACAGTATGCTGATCGCCTCGCTGATGCGATAGAGTCAGAGAACCAGTGTACATCTCAATTGAGATCACTAATTGATCAAATAAGCTTGCAACAAGGGCAAATTGAAACCATGAAAGAACAAAAGGAACAGCTAGAGCAAGAGTGTGGACAACTGAAGGCCCTTGTTCAAGAACTTGAACCTTGGAGACCTGCAACTGGTATTCCTCCACACATGACACCCCCAGTTGGTGGGACGCCTGGTCCTGGTGGTCGACCCCCAGTTGGAGGGATGCCTGGCCCTGGAGGTCGGCCCCCAGTTGGAGGGACGCCTGGTCCCGGTGGTCGACCTCCAGTTGGAGGAAGGCCACCAAAGCCAGTTCCTGCTTCTCATTTTGAGGAAAGGCCGCAGTTCAATCAGCAGAAACCAAGAGTGCCAGAATTGGAGAAACATCTTGTAGATCAACTGAGTGAAGAGGAGATTAAGTCACTAAACACAAAGTTCAAAGAAGCAACCGAAGCTGATAAAAAGGTAGGAGATCTGGAGAAAGAAATTTTCGAATCTAGAGAGAAAATCGAGTATTTCCGTGTCAAAATGCAGGAACTTGTTTTGTACAAGAGCAGATGTGACAATCGTCTTAATGAGATCACAGAAAGGGCGTCTTCCGATAGACGTGAGGCGGAGATCCTAGCCAAGAAATATGAAGAGAAATACAAACAAACTGGAGATGTAGCTTCCAAATTGACTATTGAAGAAGCCACCTTTCGTGATCTGCAGGAGAAGAAAATGGACCTATACCGGGCAATTGTCAAAATGGAGCAAGAAGGTAGTGGTGATGGCACTCTTCAGGATCGCGTTGATCGCATCCAATCTGATCTTGATGAGCTTGTGAAAACTCTCAATGAGCGCTGCAAAAAGTATGGATTACGTGCAAAACCTACAACATTAACTGAGCTTCCCTTTGGCTGGCAAGTGGGCATCCAAGAAGGAGCGGCTGACTGGGATGAAGATTGGGATAAGTTCGAAGATGAAGGATTCACTTTTGTCAAAGAGCTCACTCTTGATGTACCAAATGTCCTAGCTCCTCCAAGACAGAAATCCTCATCAGCTAAGAAGGAAAAAGCTTCTACAGCTGAGAGTCCAACAGCTGCTTCTCAGCCTAAAGTTGATGGCGAGTTGGAAAAGCCTCAAAGTACAGGTGAAAGAGTTGTTGAAAATGGAGCAGCTTATGATAAAAATGAAGACGAATCAGTAAAAAGTGTTCCAAACAGTCCATTAGCTAGCAGTACTGTTGGAAGCCCGTCTAGAGAAGTTTCAGATTCTAACTTTGGAAAGACTACAGTCTCACCCCGTGACAAGGAAACTCAAAGTGATCATGGGGGTGCTGGGTCTGTGTTTTCTGGTGACAAGAGTTTTGACGAACCAGCGTGGGGTACATTTGACGCCAATGATGACGTTGACTCGGTGTGGGGATTTAATGCAGTTAGTACCACCAAGGATACGGATCATGACGGTAATAGAGATAACTACTATTATGGTGGGTCTGGAGAGTTTGGCCTCAACCCTATCAAGACTGGGTCATCCCAAACTAGTAGCTTTTCCCAGAAGAGCCGCCCATTTACTATTGATGATTCTGTTCCTAGCACTCCACTCAATTCAGGATACTCTCCACCGAGGTTCAAAGATAGTTTAGAACCCTCATTTGACAGCTTTTCCAGGTTTGATTCCTTCAGATCACATGATAGTGGATTTTTCCCTCAAGAAAAGTTTGGAAGATTTGATTCTATGCGCAGCAGTAGAGACTTCGATCAGGGTCATGGGTTCCCATCATTTGATGACATCCCAGATCCATTTGGGTCTTCAGCACCATTTAGGACATCACTGGATAGTGAAACTCCAAGAAGGGACTCAGACCCTTTTGGTTCTTCCTCGTCATTTAGGATATCATTTGACAGTCAAACTCCAAGAAGAGACTCAGACCCTTACGGGTCTTCAGGTCCATTTAGGACATCATTTGACAGTCAAACTCCAGGAAGAGACTCGGACCGTTTTGGTTCTTCAGCGTCATTTAGGACATCATTCGATGGTCAAACTCCAAGAAGAGATTCAGACCCTTATGGGTCTTCAGCTCCATTTAGGTCGTCATTCGACAGTACTCCAAGAGCAGACTCTGACCCTTATGGATCTTCAGCTCCATTCAGGACATCATTTGACGGTCAAAGTTCAAGAAGAGATTCGGACCCTTTTGGGTCCACAGGGCCTTTTAAGCTATCGATGGAGAGTCAGACTCCGAGAAGAGACTCCGATAATTGGAGTGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

797

Amino Acids

88.36

Weight (kDa)

4.96

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNT-I PF03071 15 - 90 1.1e-09 GNT-I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1859
Acc36I ACCTGC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 320
AccBSI CCGCTC 1 cut(s) 1061
AccI GTMKAC 2 cut(s) 343, 415
AccII CGCG 2 cut(s) 83, 924
AciI CCGC 5 cut(s) 51, 476, 749, 1061, 1681
AclWI GGATC 5 cut(s) 748, 927, 1581, 1910, 2260
AcsI RAATTY 2 cut(s) 7, 621
AcuI CTGAAG 8 cut(s) 266, 899, 1783, 1914, 2046, 2112, 2178, 2241
AcyI GRCGYC 4 cut(s) 326, 398, 728, 1512
AdeI CACNNNGTG 1 cut(s) 1421
AfaI GTAC 8 cut(s) 142, 683, 1137, 1264, 1357, 1504, 1558, 2224
AfeI AGCGCT 1 cut(s) 977
AgsI TTSAA 9 cut(s) 197, 260, 269, 484, 574, 821, 1283, 1750, 2295
AhdI GACNNNNNGTC 2 cut(s) 330, 402
AhlI ACTAGT 1 cut(s) 1658
AjiI CACGTC 1 cut(s) 743
AjnI CCWGG 8 cut(s) 66, 328, 334, 364, 370, 400, 1784, 2098
AjuI GAANNNNNNNTTGG 4 cut(s) 1204, 1236, 2158, 2190
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 6 cut(s) 271, 1417, 1863, 2034, 2100, 2361
AlwI GGATC 5 cut(s) 748, 927, 1581, 1910, 2260
AlwNI CAGNNNCTG 2 cut(s) 452, 2240
Aor51HI AGCGCT 1 cut(s) 977
AoxI GGCC 7 cut(s) 249, 367, 380, 434, 473, 1624, 2327
ApeKI GCWGC 6 cut(s) 44, 47, 978, 1217, 1292, 1860
ApoI RAATTY 2 cut(s) 7, 621
ArsI GACNNNNNNTTYG 2 cut(s) 1466, 1498
Asp700I GAANNNNTTC 2 cut(s) 2173, 2302
AspLEI GCGC 2 cut(s) 978, 1860
AsuC2I CCSGG 2 cut(s) 408, 869
AsuHPI GGTGA 4 cut(s) 914, 1280, 1407, 1484
AsuII TTCGAA 2 cut(s) 627, 1092
AsuNHI GCTAGC 1 cut(s) 1349
AvaII GGWCC 7 cut(s) 332, 404, 859, 2066, 2113, 2308, 2319
BanII GRGCYC 1 cut(s) 1125
BbsI GAAGAC 5 cut(s) 723, 1317, 1920, 2052, 2184
Bbv12I GWGCWC 1 cut(s) 1125
BbvI GCAGC 6 cut(s) 34, 56, 965, 1204, 1304, 1872
BccI CCATC 5 cut(s) 899, 1232, 1903, 2147, 2338
BciT130I CCWGG 8 cut(s) 68, 330, 336, 366, 372, 402, 1786, 2100
BciVI GTATCC 2 cut(s) 1561, 1724
BclI TGATCA 2 cut(s) 166, 1441
BcnI CCSGG 2 cut(s) 408, 869
BcoDI GTCTC 6 cut(s) 271, 1417, 1863, 2034, 2100, 2361
BcuI ACTAGT 1 cut(s) 1658
BfaI CTAG 8 cut(s) 224, 633, 758, 1148, 1350, 1374, 1659, 1710
BfmI CTRYAG 3 cut(s) 842, 1197, 1407
BfoI RGCGCY 1 cut(s) 979
BfuAI ACCTGC 1 cut(s) 289
BfuI GTATCC 2 cut(s) 1561, 1724
BglII AGATCT 1 cut(s) 607
BmcAI AGTACT 2 cut(s) 1357, 2224
Bme18I GGWCC 7 cut(s) 332, 404, 859, 2066, 2113, 2308, 2319
BmeRI GACNNNNNGTC 2 cut(s) 330, 402
BmgBI CACGTC 1 cut(s) 743
BmiI GGNNCC 5 cut(s) 383, 406, 1892, 2310, 2320
BmrI ACTGGG 5 cut(s) 308, 344, 380, 1078, 1653
BmsI GCATC 4 cut(s) 106, 351, 942, 1056
BmtI GCTAGC 1 cut(s) 1353
BmuI ACTGGG 5 cut(s) 308, 344, 380, 1078, 1653
BoxI GACNNNNGTC 2 cut(s) 702, 1409
BpiI GAAGAC 5 cut(s) 723, 1317, 1920, 2052, 2184
BplI GAGNNNNNCTC 2 cut(s) 2341, 2373
BpmI CTGGAG 6 cut(s) 393, 405, 632, 813, 1635, 2082
Bpu14I TTCGAA 2 cut(s) 627, 1092
BpuEI CTTGAG 1 cut(s) 1812
BpuMI CCSGG 2 cut(s) 408, 869
Bsa29I ATCGAT 1 cut(s) 2342
BsaAI YACGTR 1 cut(s) 998
BsaBI GATNNNNATC 1 cut(s) 1572
BsaHI GRCGYC 4 cut(s) 326, 398, 728, 1512
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 4 cut(s) 272, 370, 1563, 1742
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bse8I GATNNNNATC 1 cut(s) 1572
BseBI CCWGG 8 cut(s) 68, 330, 336, 366, 372, 402, 1786, 2100
BseCI ATCGAT 1 cut(s) 2342
BseDI CCNNGG 4 cut(s) 272, 370, 1563, 1742
BseGI GGATG 6 cut(s) 366, 933, 1047, 1078, 1649, 1908
BseJI GATNNNNATC 1 cut(s) 1572
BseMII CTCAG 6 cut(s) 527, 1011, 1194, 1238, 1992, 2058
BseRI GAGGAG 2 cut(s) 560, 1143
BseXI GCAGC 6 cut(s) 34, 56, 965, 1204, 1304, 1872
BseYI CCCAGC 1 cut(s) 1454
Bsh1236I CGCG 2 cut(s) 83, 924
BshFI GGCC 7 cut(s) 251, 369, 382, 436, 475, 1626, 2329
BshVI ATCGAT 1 cut(s) 2342
BsiHKAI GWGCWC 1 cut(s) 1125
BsiSI CCGG 2 cut(s) 408, 868
BslFI GGGAC 4 cut(s) 337, 390, 409, 1988
BsmAI GTCTC 6 cut(s) 271, 1417, 1863, 2034, 2100, 2361
BsmFI GGGAC 4 cut(s) 337, 390, 409, 1988
BsnI GGCC 7 cut(s) 251, 369, 382, 436, 475, 1626, 2329
Bso31I GGTCTC 1 cut(s) 271
Bsp119I TTCGAA 2 cut(s) 627, 1092
Bsp1286I GDGCHC 1 cut(s) 1125
Bsp1407I TGTACA 2 cut(s) 140, 681
Bsp68I TCGCGA 1 cut(s) 83
BspACI CCGC 5 cut(s) 51, 476, 749, 1061, 1681
BspANI GGCC 7 cut(s) 251, 369, 382, 436, 475, 1626, 2329
BspCNI CTCAG 6 cut(s) 528, 1012, 1195, 1237, 1991, 2057
BspDI ATCGAT 1 cut(s) 2342
BspFNI CGCG 2 cut(s) 83, 924
BspHI TCATGA 1 cut(s) 1576
BspLI GGNNCC 5 cut(s) 383, 406, 1892, 2310, 2320
BspMAI CTGCAG 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 1353
BspPI GGATC 5 cut(s) 748, 927, 1581, 1910, 2260
BspQI GCTCTTC 1 cut(s) 1670
BspT104I TTCGAA 2 cut(s) 627, 1092
BspTNI GGTCTC 1 cut(s) 271
BsrBI CCGCTC 1 cut(s) 1061
BsrGI TGTACA 2 cut(s) 140, 681
BssECI CCNNGG 4 cut(s) 272, 370, 1563, 1742
BssNI GRCGYC 4 cut(s) 326, 398, 728, 1512
BssT1I CCWWGG 2 cut(s) 272, 1563
Bst2UI CCWGG 8 cut(s) 68, 330, 336, 366, 372, 402, 1786, 2100
Bst6I CTCTTC 9 cut(s) 537, 768, 918, 1670, 2032, 2098, 2164, 2293, 2359
BstACI GRCGYC 4 cut(s) 326, 398, 728, 1512
BstAUI TGTACA 2 cut(s) 140, 681
BstBAI YACGTR 1 cut(s) 998
BstBI TTCGAA 2 cut(s) 627, 1092
BstC8I GCNNGC 3 cut(s) 179, 1037, 1351
BstDEI CTNAG 7 cut(s) 536, 1020, 1179, 1203, 1224, 1978, 2044
BstF5I GGATG 6 cut(s) 366, 933, 1047, 1078, 1649, 1908
BstFNI CGCG 2 cut(s) 83, 924
BstH2I RGCGCY 1 cut(s) 979
BstHHI GCGC 2 cut(s) 978, 1860
BstMAI GTCTC 6 cut(s) 271, 1417, 1863, 2034, 2100, 2361
BstMWI GCNNNNNNNGC 8 cut(s) 24, 50, 187, 442, 587, 930, 1199, 2335
BstNI CCWGG 8 cut(s) 68, 330, 336, 366, 372, 402, 1786, 2100
BstPAI GACNNNNGTC 2 cut(s) 702, 1409
BstSFI CTRYAG 3 cut(s) 842, 1197, 1407
BstUI CGCG 2 cut(s) 83, 924
BstV1I GCAGC 6 cut(s) 34, 56, 965, 1204, 1304, 1872
BstV2I GAAGAC 5 cut(s) 723, 1317, 1920, 2052, 2184
BstX2I RGATCY 4 cut(s) 607, 753, 1915, 2252
BstYI RGATCY 4 cut(s) 607, 753, 1915, 2252
Bsu15I ATCGAT 1 cut(s) 2342
BsuI GTATCC 2 cut(s) 1561, 1724
BsuRI GGCC 7 cut(s) 251, 369, 382, 436, 475, 1626, 2329
BsuTUI ATCGAT 1 cut(s) 2342
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 6 cut(s) 366, 933, 1047, 1078, 1649, 1908
BtsIMutI CAGTG 2 cut(s) 143, 1949
BtuMI TCGCGA 1 cut(s) 83
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 179, 1037, 1351
CaiI CAGNNNCTG 2 cut(s) 452, 2240
CciI TCATGA 1 cut(s) 1576
CfoI GCGC 2 cut(s) 978, 1860
ClaI ATCGAT 1 cut(s) 2342
CpoI CGGWCCG 1 cut(s) 2113
CseI GACGC 5 cut(s) 334, 406, 717, 1520, 2121
Csp6I GTAC 8 cut(s) 141, 682, 1136, 1263, 1356, 1503, 1557, 2223
CspI CGGWCCG 1 cut(s) 2113
CviAII CATG 6 cut(s) 202, 304, 1445, 1577, 1808, 1886
CviQI GTAC 8 cut(s) 141, 682, 1136, 1263, 1356, 1503, 1557, 2223
DdeI CTNAG 7 cut(s) 536, 1020, 1179, 1203, 1224, 1978, 2044
DraIII CACNNNGTG 1 cut(s) 1421
DriI GACNNNNNGTC 2 cut(s) 330, 402
Eam1104I CTCTTC 9 cut(s) 537, 768, 918, 1670, 2032, 2098, 2164, 2293, 2359
Eam1105I GACNNNNNGTC 2 cut(s) 330, 402
EarI CTCTTC 9 cut(s) 537, 768, 918, 1670, 2032, 2098, 2164, 2293, 2359
EciI GGCGGA 1 cut(s) 764
Ecl136II GAGCTC 1 cut(s) 1123
Eco130I CCWWGG 2 cut(s) 272, 1563
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 2013
Eco47I GGWCC 7 cut(s) 332, 404, 859, 2066, 2113, 2308, 2319
Eco47III AGCGCT 1 cut(s) 977
Eco53kI GAGCTC 1 cut(s) 1123
Eco57I CTGAAG 8 cut(s) 266, 899, 1783, 1914, 2046, 2112, 2178, 2241
EcoICRI GAGCTC 1 cut(s) 1123
EcoO109I RGGNCCY 2 cut(s) 250, 2327
EcoRII CCWGG 8 cut(s) 66, 328, 334, 364, 370, 400, 1784, 2098
EcoRV GATATC 1 cut(s) 2013
EcoT14I CCWWGG 2 cut(s) 272, 1563
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 2 cut(s) 272, 1563
FaeI CATG 6 cut(s) 205, 307, 1448, 1580, 1811, 1889
FalI AAGNNNNNCTT 2 cut(s) 715, 747
FaqI GGGAC 4 cut(s) 337, 390, 409, 1988
FatI CATG 6 cut(s) 201, 303, 1444, 1576, 1807, 1885
FbaI TGATCA 2 cut(s) 166, 1441
FblI GTMKAC 2 cut(s) 343, 415
FokI GGATG 6 cut(s) 373, 920, 1034, 1085, 1636, 1895
FriOI GRGCYC 1 cut(s) 1125
FspBI CTAG 8 cut(s) 224, 633, 758, 1148, 1350, 1374, 1659, 1710
FspI TGCGCA 1 cut(s) 1859
GlaI GCGC 2 cut(s) 977, 1859
GsaI CCCAGC 1 cut(s) 1458
GsuI CTGGAG 6 cut(s) 393, 405, 632, 813, 1635, 2082
HaeII RGCGCY 1 cut(s) 979
HaeIII GGCC 7 cut(s) 251, 369, 382, 436, 475, 1626, 2329
HapII CCGG 2 cut(s) 408, 868
HgaI GACGC 5 cut(s) 334, 406, 717, 1520, 2121
HhaI GCGC 2 cut(s) 978, 1860
Hin1I GRCGYC 4 cut(s) 326, 398, 728, 1512
Hin1II CATG 6 cut(s) 205, 307, 1448, 1580, 1811, 1889
Hin6I GCGC 2 cut(s) 976, 1858
HinP1I GCGC 2 cut(s) 976, 1858
HincII GTYRAC 3 cut(s) 344, 416, 1528
HindII GTYRAC 3 cut(s) 344, 416, 1528
HindIII AAGCTT 2 cut(s) 175, 1191
HpaII CCGG 2 cut(s) 408, 868
HphI GGTGA 4 cut(s) 914, 1280, 1407, 1484
Hpy166II GTNNAC 6 cut(s) 141, 239, 344, 416, 1528, 2322
Hpy8I GTNNAC 6 cut(s) 141, 239, 344, 416, 1528, 2322
HpyAV CCTTC 9 cut(s) 64, 241, 426, 887, 1049, 1094, 1177, 1807, 1965
HpyCH4IV ACGT 3 cut(s) 742, 997, 1524
HpyCH4V TGCA 8 cut(s) 181, 284, 667, 844, 981, 1001, 1550, 2387
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 50, 187, 442, 587, 930, 1199, 2335
HpyF3I CTNAG 7 cut(s) 536, 1020, 1179, 1203, 1224, 1978, 2044
HpySE526I ACGT 3 cut(s) 742, 997, 1524
Hsp92I GRCGYC 4 cut(s) 326, 398, 728, 1512
Hsp92II CATG 6 cut(s) 205, 307, 1448, 1580, 1811, 1889
HspAI GCGC 2 cut(s) 976, 1858
Ksp22I TGATCA 2 cut(s) 166, 1441
LguI GCTCTTC 1 cut(s) 1670
LmnI GCTCC 6 cut(s) 886, 1058, 1156, 1289, 2203, 2266
Lsp1109I GCAGC 6 cut(s) 34, 56, 965, 1204, 1304, 1872
LweI GCATC 4 cut(s) 106, 351, 942, 1056
MaeI CTAG 8 cut(s) 224, 633, 758, 1148, 1350, 1374, 1659, 1710
MaeII ACGT 3 cut(s) 742, 997, 1524
MaeIII GTNAC 4 cut(s) 555, 695, 1421, 1472
MbiI CCGCTC 1 cut(s) 1061
MfeI CAATTG 2 cut(s) 149, 873
MflI RGATCY 4 cut(s) 607, 753, 1915, 2252
MhlI GDGCHC 1 cut(s) 1125
MmeI TCCRAC 6 cut(s) 334, 370, 406, 1227, 1235, 1342
MroXI GAANNNNTTC 2 cut(s) 2173, 2302
MseI TTAA 5 cut(s) 552, 708, 1016, 1545, 2334
MslI CAYNNNNRTG 2 cut(s) 1449, 1812
MspA1I CMGCKG 3 cut(s) 47, 1202, 1217
MspI CCGG 2 cut(s) 408, 868
MunI CAATTG 2 cut(s) 149, 873
MvaI CCWGG 8 cut(s) 68, 330, 336, 366, 372, 402, 1786, 2100
MvnI CGCG 2 cut(s) 83, 924
MwoI GCNNNNNNNGC 8 cut(s) 24, 50, 187, 442, 587, 930, 1199, 2335
NciI CCSGG 2 cut(s) 408, 869
NheI GCTAGC 1 cut(s) 1349
NlaIII CATG 6 cut(s) 205, 307, 1448, 1580, 1811, 1889
NlaIV GGNNCC 5 cut(s) 383, 406, 1892, 2310, 2320
NmuCI GTSAC 4 cut(s) 555, 695, 1421, 1472
NruI TCGCGA 1 cut(s) 83
NsbI TGCGCA 1 cut(s) 1859
NspV TTCGAA 2 cut(s) 627, 1092
PagI TCATGA 1 cut(s) 1576
PciSI GCTCTTC 1 cut(s) 1670
PdmI GAANNNNTTC 2 cut(s) 2173, 2302
PfeI GAWTC 9 cut(s) 629, 1104, 1316, 1388, 1700, 1793, 1850, 2174, 2303
PflFI GACNNNGTC 1 cut(s) 1645
PflMI CCANNNNNTGG 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 2098
Ppu21I YACGTR 1 cut(s) 998
PshAI GACNNNNGTC 2 cut(s) 702, 1409
Psp124BI GAGCTC 1 cut(s) 1125
Psp6I CCWGG 8 cut(s) 66, 328, 334, 364, 370, 400, 1784, 2098
PspFI CCCAGC 1 cut(s) 1454
PspGI CCWGG 8 cut(s) 66, 328, 334, 364, 370, 400, 1784, 2098
PspN4I GGNNCC 5 cut(s) 383, 406, 1892, 2310, 2320
PsrI GAACNNNNNNTAC 2 cut(s) 1314, 1346
PstI CTGCAG 1 cut(s) 846
PstNI CAGNNNCTG 2 cut(s) 452, 2240
PsuI RGATCY 4 cut(s) 607, 753, 1915, 2252
PsyI GACNNNGTC 1 cut(s) 1645
PvuII CAGCTG 3 cut(s) 47, 1202, 1217
RruI TCGCGA 1 cut(s) 83
RsaI GTAC 8 cut(s) 142, 683, 1137, 1264, 1357, 1504, 1558, 2224
RsaNI GTAC 8 cut(s) 141, 682, 1136, 1263, 1356, 1503, 1557, 2223
RseI CAYNNNNRTG 2 cut(s) 1449, 1812
Rsr2I CGGWCCG 1 cut(s) 2113
RsrII CGGWCCG 1 cut(s) 2113
SacI GAGCTC 1 cut(s) 1125
SalI GTCGAC 2 cut(s) 342, 414
SapI GCTCTTC 1 cut(s) 1670
SaqAI TTAA 5 cut(s) 552, 708, 1016, 1545, 2334
ScaI AGTACT 2 cut(s) 1357, 2224
SduI GDGCHC 1 cut(s) 1125
SfaNI GCATC 4 cut(s) 106, 351, 942, 1056
SfcI CTRYAG 3 cut(s) 842, 1197, 1407
SfuI TTCGAA 2 cut(s) 627, 1092
SinI GGWCC 7 cut(s) 332, 404, 859, 2066, 2113, 2308, 2319
SmiMI CAYNNNNRTG 2 cut(s) 1449, 1812
SmlI CTYRAG 1 cut(s) 1827
SmoI CTYRAG 1 cut(s) 1827
SpeI ACTAGT 1 cut(s) 1658
SsiI CCGC 5 cut(s) 51, 476, 749, 1061, 1681
SspMI CTAG 8 cut(s) 224, 633, 758, 1148, 1350, 1374, 1659, 1710
SstI GAGCTC 1 cut(s) 1125
StyI CCWWGG 2 cut(s) 272, 1563
TaiI ACGT 3 cut(s) 745, 1000, 1527
TaqI TCGA 9 cut(s) 343, 415, 627, 645, 1092, 1875, 2151, 2217, 2342
TatI WGTACW 5 cut(s) 140, 681, 1262, 1355, 2222
TauI GCSGC 4 cut(s) 53, 478, 1064, 1683
TfiI GAWTC 9 cut(s) 629, 1104, 1316, 1388, 1700, 1793, 1850, 2174, 2303
Tru1I TTAA 5 cut(s) 552, 708, 1016, 1545, 2334
Tru9I TTAA 5 cut(s) 552, 708, 1016, 1545, 2334
TscAI CASTG 2 cut(s) 143, 1956
TseFI GTSAC 4 cut(s) 555, 695, 1421, 1472
TseI GCWGC 6 cut(s) 44, 47, 978, 1217, 1292, 1860
Tsp45I GTSAC 4 cut(s) 555, 695, 1421, 1472
TspDTI ATGAA 7 cut(s) 17, 46, 218, 786, 1089, 1113, 1323
TspGWI ACGGA 2 cut(s) 644, 1586
TspRI CASTG 2 cut(s) 143, 1956
Tth111I GACNNNGTC 1 cut(s) 1645
Van91I CCANNNNNTGG 1 cut(s) 320
VpaK11BI GGWCC 7 cut(s) 332, 404, 859, 2066, 2113, 2308, 2319
XapI RAATTY 2 cut(s) 7, 621
XbaI TCTAGA 2 cut(s) 632, 1373
XmiI GTMKAC 2 cut(s) 343, 415
XmnI GAANNNNTTC 2 cut(s) 2173, 2302
XspI CTAG 8 cut(s) 224, 633, 758, 1148, 1350, 1374, 1659, 1710
ZrmI AGTACT 2 cut(s) 1357, 2224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.