Rh6AG248700

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004010
Physical Location & Seq
Forward (+)
43851585 .. 43862881
11297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG248700.1

Sequence Viewer

Length: 3213 bp
ATGAACCACCAAGCTTTCCTTTCTCAAGGTCAGACAATGAGGCCACCAGTGCCTCAATCTACTACTGCTGCTTCCCAACTGACGCAGGGTGTTTCTTCACAAGGGTTCTCCCAGGGAGTTTCAGTGGTAGGTTCTCGTCCACCAAACTCGAGCATGTCAAATGATTGGGTTGGAGGAAGGGCAGGTGGATCTGTGACAGGGATGCACTCACAAGTCTTAAATAGAGGGATCACTCCATCTGCAGCACAGGATGGATTTGGGCTGGCAACATCTGGACCAACAGTTTCCGCACTCTCTAGACCACAGGCACCTTCTGGCATGACACCATCAGGACCACCGGCTAAGGATTCCAAATCATTAAATGTTTCTGGGAATGGCTTTGCTCCTGACTCATCTTTTGGAGATGATGTGTTTTCTGCAATCCCATCTCAACCAAAGCAAAACACTTCTACAAATTCATTTCCTTCTGGCAGCATACCAGTCTCATCTGCCGTTGTACCAGTATCTGCCGGGCCCCAATCATCTTCTCATGCATTTCCGGGTGGCAGTGTTCCATTCTCATCGGCCATTGTTCCAGTTACTTCTGGGCCTCAATCTTCAGAGAGGCCAAGTACCGTTTCACCCATGCAGCCTGTTGGTGGCCAACCTCAGCAGCCCCGGTCATTTGCATCATCAAACCAGCAGGTCCCCACCCAGACTACAACATCTATCACATCTGGAGTTTCATATGGAGCAGGGAACTCTGCTTCTGGTCAATCCTGGCCAAGGATGACTCAAACTGATGTTCAGAAGTACTCAAATATTTTTGTGAAAGTCGACACAGACAGAGATGGGAAAATCACTGGTGATCAAGCTCGTGACTTATTTTTGAAATGGGGATTGCCAAGAGAGATTTTAAAGCAGGTGTGGGACTTATCTGATCAAGATAATGATAGCATGCTTTCTCTCAAGGAGTTCTGTATCGCACTATATTTGATGGAGCGATATAGGGAGGGACGTCCTCTTCCAGCAGCTCTCCCAAGCAGTGTTTTGTTTGATTTATCCGGTATTGTTCAACCTGCAAATAATTACAGCAATGCTGGCAATGTAGCTTGGAGACCTGCAACTGGTATTCCTCCACACATGACACCCCCAGTTGGTGGGACGCCTGGTCCTGGTGGTCGACCCCCAGTTGGAGGGATGCCTGGCCCTGGAGGTCGGCCCCCAGTTGGAGGGATGCCTGGTCCCGGTGGTCGACCTCCAGTTGGAGGAAGGCCACCAAAGCCAGTTCCTGCTTCTCATTTTGAGGAAAGGCCGCAGATCAATCAGCAGAAACCAAGAGTGCCAGAATTGGAGAAACATCTTGTAGATCAACTGAGTGAAGGGGAGATTAAGTCACTAAACACAAAGTTCAAAGAAGCAACCGAAGCTGATAAAAAGGTAGGAGATCTGGAGAAAGAAATTTTCGAATCTAGAGAGAAAATCGAGTATTTCCGTGTCAAAATGCAGGAACTTGTTTTGTACAAGAGCAGATGTGACAATCGTCTTAATGAGATCACAGAAAGGGCGTCTTCCGATAGACGTGAGGCGGAGATCCTCGCCAAGAAATATGAAGAGAAATACAAACAAACTGGAGATGTAGCTTCCAAATTGACTATTGAAGAAGCCACCTTTCGTGATCTGCAGGAGAAGAAAATGGACCTATACCGGGCAATTGTCAAAATGGAGCAAGAAGGTAGTGGTGATGGCACTCTTCAGGATCGCGTTGATCACATCCAATCTGATCTTGATGAGCTTGTGAAAACTCTCAATGAGCGCTGCAAAAAGTATGGATTACGTGCAAAACCTACAACATTAACTGAGCTTCCCTTTGGCTGGCAAGTGGGCATCCAAGAAGGAGCGGCTGACTGGGATGAAGATTGGGATAAGTTCGAAGATGAAGGATTCACTTTTGTCAAAGAGCTCACTCTTGATGTACCAAATGTACTAGCTCCTCCAAGACAGAAATCCTCTTCAGCTAAGAAGGAAAAAGCTTCTACAGCTGAGAGTCCAACAGCTGCTTCTCAGCCTAAAGTTGATGGCGAGTCGGAAAAGCCTCAAAGTACAGGTGAAAGAGTTGTTGAAAATGGAGCAGCTTATGATAAAAATGAAGGCGAATCAGTAAAAAGTGTTCCAAACAGTCCATTAGCTAGCAGTACTGTTGGAAGCCCGTCTAGAGAAGTTTCAGATTCTAACTTTGGAAAGACTGCAGTCTCACCCCGTGACAAGGAAACTCAAAGTGATCATGGGGGTGCTGGGTCTGTGTTTTCTGGTGACAAGAGTTTTGACGAACCAGCGTGGGGTACATTTGACGCCAATGATGACGTTGACTCGGTGTGGGGATTTAATGCAGTTAGTACCACCAAGGATACGGATCATGACGGTCATAGAGATAACTACTATTATGGTGGGTCTGGAGAGTTTGGCCTCAACCCTATCAAGACTGGGTCATCCCAAACTAGTAGCTTTTCCCAGAAGAGCCGCCCATTTACTTTTGATGATTCTGTTCCTAGCACTCCACTCAATTCAGGATACTCTCCACCAAGGTTCAAAGATAGTTTAGAACCCTCATTTGACAGCTTTTCCAGGTTTGATTCCTTCAGATCACATGATAGTGGATTTTTCCCTCAAGAAAAGTTTGGAAGATTTGATTCTATGCGCAGCAGTAGAGACTTCGATCAGGGTCATGGGTTCCCATCATTTGATGACATCCCAGATCCATTTGGGTCTTCAGCACCATTTAGGACATCACTGGATGGTGAAACTCCAAGAATGGACTCAGACCCTTTTGGTTCTTCCTCGTCATTTAGGATATCATTTGACAGTCAAACTCCAAGAAGAGACTCAGACCCTTATGGGTCTTCAGGTCCATTTAGGACATCATTTGACAGTCAAACTCCAGGAAGAGACTCGGACCGTTTTGGTTCTTCAGCGTCATTTAGGACATCATTCGATGGTCAAACTCCAAGAAGAGATTCAGACCCTTATGGGTCTTCAGCTCCATTTAGGTCGTCATTCGACAGTACTCCAAGAGCAGACTCTGACCCTTATGGATCTTCAGCTCCATTCAGGACATCATTTGACGGTCAAAGTTCAAGAAGAGATTCGGACCCTTTTGGGTCCACAGGGCCTTTTAAGCTATCGATGGAGAGTCAGACTCCGAGAAGAGACTCTGATAATTGGAGTGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

1070

Amino Acids

115.48

Weight (kDa)

5.26

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EH PF12763 258 - 332 2.8e-14 EH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 173, 892
AatII GACGTC 1 cut(s) 1000
Acc16I TGCGCA 1 cut(s) 2678
Acc36I ACCTGC 5 cut(s) 173, 673, 892, 1066, 1108
AccB1I GGYRCC 1 cut(s) 307
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccBSI CCGCTC 1 cut(s) 1880
AccI GTMKAC 3 cut(s) 816, 1162, 1234
AccII CGCG 1 cut(s) 1743
AciI CCGC 5 cut(s) 288, 1295, 1568, 1880, 2500
AclWI GGATC 7 cut(s) 196, 236, 1567, 1746, 2400, 2729, 3079
AcoI YGGCCR 3 cut(s) 564, 640, 761
AcsI RAATTY 2 cut(s) 454, 1440
AcuI CTGAAG 9 cut(s) 582, 1718, 1977, 2602, 2733, 2865, 2931, 2997, 3060
AcyI GRCGYC 4 cut(s) 997, 1145, 1547, 2331
AdeI CACNNNGTG 1 cut(s) 2240
AfeI AGCGCT 1 cut(s) 1796
AgsI TTSAA 7 cut(s) 871, 1055, 1393, 1640, 2102, 2569, 3114
AhdI GACNNNNNGTC 1 cut(s) 1149
AhlI ACTAGT 1 cut(s) 2477
AjiI CACGTC 1 cut(s) 1562
AjnI CCWGG 9 cut(s) 111, 758, 1147, 1153, 1183, 1189, 1219, 2603, 2917
AjuI GAANNNNNNNTTGG 6 cut(s) 601, 633, 2023, 2055, 2977, 3009
Alw21I GWGCWC 1 cut(s) 1944
Alw26I GTCTC 7 cut(s) 487, 1090, 2236, 2682, 2853, 2919, 3180
AlwI GGATC 7 cut(s) 196, 236, 1567, 1746, 2400, 2729, 3079
AlwNI CAGNNNCTG 3 cut(s) 506, 1271, 3059
Ama87I CYCGRG 1 cut(s) 148
Aor51HI AGCGCT 1 cut(s) 1796
ApaI GGGCCC 1 cut(s) 516
ApoI RAATTY 2 cut(s) 454, 1440
ArsI GACNNNNNNTTYG 4 cut(s) 380, 412, 2285, 2317
Asp700I GAANNNNTTC 2 cut(s) 2992, 3121
AspLEI GCGC 2 cut(s) 1797, 2679
AsuC2I CCSGG 5 cut(s) 511, 540, 658, 1227, 1688
AsuHPI GGTGA 7 cut(s) 612, 857, 1733, 2099, 2226, 2303, 2789
AsuII TTCGAA 2 cut(s) 1446, 1911
AsuNHI GCTAGC 1 cut(s) 2168
AvaI CYCGRG 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 516
BalI TGGCCA 2 cut(s) 642, 763
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 2 cut(s) 516, 1944
BauI CACGAG 1 cut(s) 855
BbsI GAAGAC 4 cut(s) 1542, 2739, 2871, 3003
Bbv12I GWGCWC 1 cut(s) 1944
BbvCI CCTCAGC 1 cut(s) 648
BceAI ACGGC 1 cut(s) 476
BciT130I CCWGG 9 cut(s) 113, 760, 1149, 1155, 1185, 1191, 1221, 2605, 2919
BciVI GTATCC 2 cut(s) 2380, 2543
BclI TGATCA 4 cut(s) 847, 919, 1747, 2260
BcnI CCSGG 5 cut(s) 511, 540, 658, 1227, 1688
BcoDI GTCTC 7 cut(s) 487, 1090, 2236, 2682, 2853, 2919, 3180
BcuI ACTAGT 1 cut(s) 2477
BfaI CTAG 7 cut(s) 297, 1452, 1967, 2169, 2193, 2478, 2529
BfmI CTRYAG 4 cut(s) 240, 1661, 2016, 2226
BfoI RGCGCY 1 cut(s) 1798
BfuAI ACCTGC 5 cut(s) 173, 673, 892, 1066, 1108
BfuI GTATCC 2 cut(s) 2380, 2543
BglII AGATCT 1 cut(s) 1426
BmcAI AGTACT 3 cut(s) 794, 2176, 3043
BmeRI GACNNNNNGTC 1 cut(s) 1149
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 1562
BmiI GGNNCC 9 cut(s) 309, 514, 515, 687, 1202, 1225, 2711, 3129, 3139
BmrI ACTGGG 5 cut(s) 1127, 1163, 1199, 1897, 2472
BmsI GCATC 5 cut(s) 192, 677, 1170, 1206, 1875
BmtI GCTAGC 1 cut(s) 2172
BmuI ACTGGG 5 cut(s) 1127, 1163, 1199, 1897, 2472
BoxI GACNNNNGTC 2 cut(s) 1521, 2228
BpiI GAAGAC 4 cut(s) 1542, 2739, 2871, 3003
BplI GAGNNNNNCTC 2 cut(s) 3160, 3192
BpmI CTGGAG 7 cut(s) 738, 1212, 1224, 1451, 1632, 2454, 2901
Bpu10I CCTNAGC 2 cut(s) 342, 648
Bpu14I TTCGAA 2 cut(s) 1446, 1911
BpuEI CTTGAG 3 cut(s) 9, 932, 2631
BpuMI CCSGG 5 cut(s) 511, 540, 658, 1227, 1688
Bsa29I ATCGAT 1 cut(s) 3161
BsaAI YACGTR 1 cut(s) 1817
BsaBI GATNNNNATC 1 cut(s) 2391
BsaHI GRCGYC 4 cut(s) 997, 1145, 1547, 2331
BsaI GGTCTC 1 cut(s) 1090
BsaJI CCNNGG 7 cut(s) 111, 112, 656, 764, 1189, 2382, 2561
BsaWI WCCGGW 1 cut(s) 1043
BsaXI ACNNNNNCTCC 6 cut(s) 108, 138, 393, 423, 1358, 1388
Bse118I RCCGGY 1 cut(s) 337
Bse3DI GCAATG 2 cut(s) 1081, 1090
Bse8I GATNNNNATC 1 cut(s) 2391
BseBI CCWGG 9 cut(s) 113, 760, 1149, 1155, 1185, 1191, 1221, 2605, 2919
BseCI ATCGAT 1 cut(s) 3161
BseDI CCNNGG 7 cut(s) 111, 112, 656, 764, 1189, 2382, 2561
BseJI GATNNNNATC 1 cut(s) 2391
BseMI GCAATG 2 cut(s) 1081, 1090
BseMII CTCAG 7 cut(s) 662, 1346, 1830, 2013, 2057, 2811, 2877
BseRI GAGGAG 1 cut(s) 1962
BseSI GKGCMC 1 cut(s) 516
BseYI CCCAGC 1 cut(s) 2273
Bsh1236I CGCG 1 cut(s) 1743
BshNI GGYRCC 1 cut(s) 307
BshVI ATCGAT 1 cut(s) 3161
BsiHKAI GWGCWC 1 cut(s) 1944
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 7 cut(s) 338, 510, 539, 658, 1044, 1227, 1687
BslFI GGGAC 5 cut(s) 671, 923, 1008, 1156, 1209
BsmAI GTCTC 7 cut(s) 487, 1090, 2236, 2682, 2853, 2919, 3180
BsmFI GGGAC 5 cut(s) 671, 923, 1008, 1156, 1209
Bso31I GGTCTC 1 cut(s) 1090
BsoBI CYCGRG 1 cut(s) 148
Bsp119I TTCGAA 2 cut(s) 1446, 1911
Bsp120I GGGCCC 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 516, 1944
Bsp1407I TGTACA 1 cut(s) 1500
BspACI CCGC 5 cut(s) 288, 1295, 1568, 1880, 2500
BspCNI CTCAG 7 cut(s) 661, 1347, 1831, 2014, 2056, 2810, 2876
BspDI ATCGAT 1 cut(s) 3161
BspFNI CGCG 1 cut(s) 1743
BspHI TCATGA 1 cut(s) 2395
BspLI GGNNCC 9 cut(s) 309, 514, 515, 687, 1202, 1225, 2711, 3129, 3139
BspMAI CTGCAG 3 cut(s) 244, 1665, 2230
BspMI ACCTGC 5 cut(s) 173, 673, 892, 1066, 1108
BspOI GCTAGC 1 cut(s) 2172
BspPI GGATC 7 cut(s) 196, 236, 1567, 1746, 2400, 2729, 3079
BspQI GCTCTTC 1 cut(s) 2489
BspT104I TTCGAA 2 cut(s) 1446, 1911
BspT107I GGYRCC 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 1090
BsrBI CCGCTC 1 cut(s) 1880
BsrDI GCAATG 2 cut(s) 1081, 1090
BsrFI RCCGGY 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 1500
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 7 cut(s) 111, 112, 656, 764, 1189, 2382, 2561
BssNI GRCGYC 4 cut(s) 997, 1145, 1547, 2331
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 3 cut(s) 764, 2382, 2561
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 9 cut(s) 113, 760, 1149, 1155, 1185, 1191, 1221, 2605, 2919
BstACI GRCGYC 4 cut(s) 997, 1145, 1547, 2331
BstAUI TGTACA 1 cut(s) 1500
BstBAI YACGTR 1 cut(s) 1817
BstBI TTCGAA 2 cut(s) 1446, 1911
BstC8I GCNNGC 5 cut(s) 264, 938, 1081, 1856, 2170
BstDEI CTNAG 9 cut(s) 342, 648, 1355, 1839, 1998, 2022, 2043, 2797, 2863
BstENI CCTNNNNNAGG 1 cut(s) 763
BstFNI CGCG 1 cut(s) 1743
BstH2I RGCGCY 1 cut(s) 1798
BstHHI GCGC 2 cut(s) 1797, 2679
BstMAI GTCTC 7 cut(s) 487, 1090, 2236, 2682, 2853, 2919, 3180
BstMWI GCNNNNNNNGC 6 cut(s) 49, 1080, 1261, 1406, 2018, 3154
BstNI CCWGG 9 cut(s) 113, 760, 1149, 1155, 1185, 1191, 1221, 2605, 2919
BstNSI RCATGY 2 cut(s) 157, 940
BstPAI GACNNNNGTC 2 cut(s) 1521, 2228
BstSFI CTRYAG 4 cut(s) 240, 1661, 2016, 2226
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 1743
BstV2I GAAGAC 4 cut(s) 1542, 2739, 2871, 3003
BstX2I RGATCY 5 cut(s) 188, 1426, 1572, 2734, 3071
BstYI RGATCY 5 cut(s) 188, 1426, 1572, 2734, 3071
Bsu15I ATCGAT 1 cut(s) 3161
BsuI GTATCC 2 cut(s) 2380, 2543
BsuTUI ATCGAT 1 cut(s) 3161
BtrI CACGTC 1 cut(s) 1562
BtsI GCAGTG 2 cut(s) 553, 1030
BtsIMutI CAGTG 6 cut(s) 54, 129, 553, 840, 1030, 2768
BveI ACCTGC 5 cut(s) 173, 673, 892, 1066, 1108
Cac8I GCNNGC 5 cut(s) 264, 938, 1081, 1856, 2170
CaiI CAGNNNCTG 3 cut(s) 506, 1271, 3059
CciI TCATGA 1 cut(s) 2395
CfoI GCGC 2 cut(s) 1797, 2679
Cfr10I RCCGGY 1 cut(s) 337
ClaI ATCGAT 1 cut(s) 3161
CpoI CGGWCCG 1 cut(s) 2932
CseI GACGC 5 cut(s) 91, 1153, 1536, 2339, 2940
CspI CGGWCCG 1 cut(s) 2932
DdeI CTNAG 9 cut(s) 342, 648, 1355, 1839, 1998, 2022, 2043, 2797, 2863
DraI TTTAAA 1 cut(s) 897
DraIII CACNNNGTG 1 cut(s) 2240
DriI GACNNNNNGTC 1 cut(s) 1149
EaeI YGGCCR 3 cut(s) 564, 640, 761
Eam1105I GACNNNNNGTC 1 cut(s) 1149
EciI GGCGGA 1 cut(s) 1583
Ecl136II GAGCTC 1 cut(s) 1942
Eco130I CCWWGG 3 cut(s) 764, 2382, 2561
Eco24I GRGCYC 2 cut(s) 516, 1944
Eco31I GGTCTC 1 cut(s) 1090
Eco32I GATATC 1 cut(s) 2832
Eco47III AGCGCT 1 cut(s) 1796
Eco53kI GAGCTC 1 cut(s) 1942
Eco57I CTGAAG 9 cut(s) 582, 1718, 1977, 2602, 2733, 2865, 2931, 2997, 3060
Eco88I CYCGRG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 1942
EcoNI CCTNNNNNAGG 1 cut(s) 763
EcoO109I RGGNCCY 3 cut(s) 513, 685, 3146
EcoRII CCWGG 9 cut(s) 111, 758, 1147, 1153, 1183, 1189, 1219, 2603, 2917
EcoRV GATATC 1 cut(s) 2832
EcoT14I CCWWGG 3 cut(s) 764, 2382, 2561
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 2 cut(s) 516, 1944
ErhI CCWWGG 3 cut(s) 764, 2382, 2561
FalI AAGNNNNNCTT 3 cut(s) 35, 1534, 1566
FaqI GGGAC 5 cut(s) 671, 923, 1008, 1156, 1209
FauNDI CATATG 1 cut(s) 727
FbaI TGATCA 4 cut(s) 847, 919, 1747, 2260
FblI GTMKAC 3 cut(s) 816, 1162, 1234
FriOI GRGCYC 2 cut(s) 516, 1944
FspBI CTAG 7 cut(s) 297, 1452, 1967, 2169, 2193, 2478, 2529
FspI TGCGCA 1 cut(s) 2678
GlaI GCGC 2 cut(s) 1796, 2678
GsaI CCCAGC 1 cut(s) 2277
GsuI CTGGAG 7 cut(s) 738, 1212, 1224, 1451, 1632, 2454, 2901
HaeII RGCGCY 1 cut(s) 1798
HapII CCGG 7 cut(s) 338, 510, 539, 658, 1044, 1227, 1687
HgaI GACGC 5 cut(s) 91, 1153, 1536, 2339, 2940
HhaI GCGC 2 cut(s) 1797, 2679
Hin1I GRCGYC 4 cut(s) 997, 1145, 1547, 2331
Hin6I GCGC 2 cut(s) 1795, 2677
HinP1I GCGC 2 cut(s) 1795, 2677
HincII GTYRAC 4 cut(s) 817, 1163, 1235, 2347
HindII GTYRAC 4 cut(s) 817, 1163, 1235, 2347
HindIII AAGCTT 2 cut(s) 12, 2010
HpaII CCGG 7 cut(s) 338, 510, 539, 658, 1044, 1227, 1687
HphI GGTGA 7 cut(s) 612, 857, 1733, 2099, 2226, 2303, 2789
Hpy166II GTNNAC 6 cut(s) 140, 817, 1163, 1235, 2347, 3141
Hpy8I GTNNAC 6 cut(s) 140, 817, 1163, 1235, 2347, 3141
HpyCH4IV ACGT 4 cut(s) 997, 1561, 1816, 2343
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 1080, 1261, 1406, 2018, 3154
HpyF3I CTNAG 9 cut(s) 342, 648, 1355, 1839, 1998, 2022, 2043, 2797, 2863
HpySE526I ACGT 4 cut(s) 997, 1561, 1816, 2343
Hsp92I GRCGYC 4 cut(s) 997, 1145, 1547, 2331
HspAI GCGC 2 cut(s) 1795, 2677
Ksp22I TGATCA 4 cut(s) 847, 919, 1747, 2260
LguI GCTCTTC 1 cut(s) 2489
LmnI GCTCC 9 cut(s) 388, 731, 979, 1705, 1877, 1975, 2108, 3022, 3085
LweI GCATC 5 cut(s) 192, 677, 1170, 1206, 1875
MaeI CTAG 7 cut(s) 297, 1452, 1967, 2169, 2193, 2478, 2529
MaeII ACGT 4 cut(s) 997, 1561, 1816, 2343
MaeIII GTNAC 7 cut(s) 193, 577, 857, 1374, 1514, 2240, 2291
MbiI CCGCTC 1 cut(s) 1880
MfeI CAATTG 1 cut(s) 1692
MflI RGATCY 5 cut(s) 188, 1426, 1572, 2734, 3071
MhlI GDGCHC 2 cut(s) 516, 1944
MlsI TGGCCA 2 cut(s) 642, 763
MluCI AATT 8 cut(s) 454, 1066, 1328, 1440, 1628, 1692, 2542, 3196
MluNI TGGCCA 2 cut(s) 642, 763
MmeI TCCRAC 7 cut(s) 151, 1153, 1189, 1225, 2046, 2054, 2161
Mox20I TGGCCA 2 cut(s) 642, 763
Mph1103I ATGCAT 1 cut(s) 535
MroXI GAANNNNTTC 2 cut(s) 2992, 3121
MscI TGGCCA 2 cut(s) 642, 763
MseI TTAA 8 cut(s) 218, 359, 896, 1371, 1527, 1835, 2364, 3153
MslI CAYNNNNRTG 2 cut(s) 2268, 2631
Msp20I TGGCCA 2 cut(s) 642, 763
MspA1I CMGCKG 2 cut(s) 2021, 2036
MspI CCGG 7 cut(s) 338, 510, 539, 658, 1044, 1227, 1687
MunI CAATTG 1 cut(s) 1692
MvaI CCWGG 9 cut(s) 113, 760, 1149, 1155, 1185, 1191, 1221, 2605, 2919
MvnI CGCG 1 cut(s) 1743
MwoI GCNNNNNNNGC 6 cut(s) 49, 1080, 1261, 1406, 2018, 3154
NciI CCSGG 5 cut(s) 511, 540, 658, 1227, 1688
NdeI CATATG 1 cut(s) 727
NheI GCTAGC 1 cut(s) 2168
NlaIV GGNNCC 9 cut(s) 309, 514, 515, 687, 1202, 1225, 2711, 3129, 3139
NmuCI GTSAC 6 cut(s) 193, 857, 1374, 1514, 2240, 2291
NsbI TGCGCA 1 cut(s) 2678
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 2 cut(s) 157, 940
NspV TTCGAA 2 cut(s) 1446, 1911
PaeI GCATGC 1 cut(s) 940
PaeR7I CTCGAG 1 cut(s) 148
PagI TCATGA 1 cut(s) 2395
PaqCI CACCTGC 2 cut(s) 173, 892
PasI CCCWGGG 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 2489
PdmI GAANNNNTTC 2 cut(s) 2992, 3121
PflFI GACNNNGTC 1 cut(s) 2464
PflMI CCANNNNNTGG 1 cut(s) 1139
PfoI TCCNGGA 1 cut(s) 2917
Ppu21I YACGTR 1 cut(s) 1817
PpuMI RGGWCCY 1 cut(s) 685
PshAI GACNNNNGTC 2 cut(s) 1521, 2228
Psp124BI GAGCTC 1 cut(s) 1944
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 9 cut(s) 111, 758, 1147, 1153, 1183, 1189, 1219, 2603, 2917
PspFI CCCAGC 1 cut(s) 2273
PspGI CCWGG 9 cut(s) 111, 758, 1147, 1153, 1183, 1189, 1219, 2603, 2917
PspN4I GGNNCC 9 cut(s) 309, 514, 515, 687, 1202, 1225, 2711, 3129, 3139
PspOMI GGGCCC 1 cut(s) 512
PspPPI RGGWCCY 1 cut(s) 685
PspXI VCTCGAGB 1 cut(s) 148
PsrI GAACNNNNNNTAC 2 cut(s) 2133, 2165
PstI CTGCAG 3 cut(s) 244, 1665, 2230
PstNI CAGNNNCTG 3 cut(s) 506, 1271, 3059
PsuI RGATCY 5 cut(s) 188, 1426, 1572, 2734, 3071
PsyI GACNNNGTC 1 cut(s) 2464
PvuII CAGCTG 2 cut(s) 2021, 2036
RseI CAYNNNNRTG 2 cut(s) 2268, 2631
Rsr2I CGGWCCG 1 cut(s) 2932
RsrII CGGWCCG 1 cut(s) 2932
SacI GAGCTC 1 cut(s) 1944
SalI GTCGAC 3 cut(s) 815, 1161, 1233
SapI GCTCTTC 1 cut(s) 2489
SaqAI TTAA 8 cut(s) 218, 359, 896, 1371, 1527, 1835, 2364, 3153
ScaI AGTACT 3 cut(s) 794, 2176, 3043
SduI GDGCHC 2 cut(s) 516, 1944
SfaNI GCATC 5 cut(s) 192, 677, 1170, 1206, 1875
SfcI CTRYAG 4 cut(s) 240, 1661, 2016, 2226
Sfr274I CTCGAG 1 cut(s) 148
SfuI TTCGAA 2 cut(s) 1446, 1911
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 2268, 2631
SmlI CTYRAG 4 cut(s) 24, 148, 947, 2646
SmoI CTYRAG 4 cut(s) 24, 148, 947, 2646
SpeI ACTAGT 1 cut(s) 2477
SphI GCATGC 1 cut(s) 940
Sse9I AATT 8 cut(s) 454, 1066, 1328, 1440, 1628, 1692, 2542, 3196
SsiI CCGC 5 cut(s) 288, 1295, 1568, 1880, 2500
SspI AATATT 1 cut(s) 802
SspMI CTAG 7 cut(s) 297, 1452, 1967, 2169, 2193, 2478, 2529
SstI GAGCTC 1 cut(s) 1944
StyI CCWWGG 3 cut(s) 764, 2382, 2561
TaiI ACGT 4 cut(s) 1000, 1564, 1819, 2346
TasI AATT 8 cut(s) 454, 1066, 1328, 1440, 1628, 1692, 2542, 3196
TatI WGTACW 6 cut(s) 792, 1500, 1963, 2081, 2174, 3041
TauI GCSGC 3 cut(s) 1297, 1883, 2502
Tru1I TTAA 8 cut(s) 218, 359, 896, 1371, 1527, 1835, 2364, 3153
Tru9I TTAA 8 cut(s) 218, 359, 896, 1371, 1527, 1835, 2364, 3153
TscAI CASTG 6 cut(s) 54, 129, 553, 847, 1030, 2775
TseFI GTSAC 6 cut(s) 193, 857, 1374, 1514, 2240, 2291
Tsp45I GTSAC 6 cut(s) 193, 857, 1374, 1514, 2240, 2291
TspDTI ATGAA 7 cut(s) 17, 447, 714, 1605, 1908, 1932, 2142
TspGWI ACGGA 2 cut(s) 1463, 2405
TspRI CASTG 6 cut(s) 54, 129, 553, 847, 1030, 2775
Tth111I GACNNNGTC 1 cut(s) 2464
Van91I CCANNNNNTGG 1 cut(s) 1139
XagI CCTNNNNNAGG 1 cut(s) 763
XapI RAATTY 2 cut(s) 454, 1440
XbaI TCTAGA 3 cut(s) 296, 1451, 2192
XceI RCATGY 2 cut(s) 157, 940
XhoI CTCGAG 1 cut(s) 148
XmiI GTMKAC 3 cut(s) 816, 1162, 1234
XmnI GAANNNNTTC 2 cut(s) 2992, 3121
XspI CTAG 7 cut(s) 297, 1452, 1967, 2169, 2193, 2478, 2529
ZraI GACGTC 1 cut(s) 998
ZrmI AGTACT 3 cut(s) 794, 2176, 3043
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.