Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007271

Physical Location & Seq

Genomic Coordinates Reverse (-)

28246134 .. 28250319

Gene Structure 4186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1151600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGCTCCTGCTGCTGCTGGAGCTGGATTGGAATAGGATGTACGACGGCGAAAAGCCAGTTCCAACAGAGAACAAAAGCTCCAAACCTGAAACAACAGAGAACAAAAGTTCAAAGTCTGAGGCATCGTCTGGAGACTCACTGTCAGGACAACCAGGGGCTACCCCTTTCCAACTTCCAGAGGGTGGATTTCCTCCCAATCCTTTTGATTTCTCAGCCATGACTGGCTTGCTGAATGATCCTAGCATAAAGGAACTAGCTGAACAGATATCAAAAGATCCTTCATTCAATCAGATGGCAGAACAACTTCAGAAAACTTTTCAAGGTGTGTCAGTTGATGAAGGTGTCCCTCAGTTTGATAGCCAAGAATACTATTCCACCATGCAACAGGTTATGCAGAATCCTCAGTTCATGACCATGGCCGAGCGCCTCGGTAATGCATTGATGCAGGATCCATCTATGAATACCATGCTTGAGAGTTTCGCCAATCCATCAAGCAAAGATCAGCTTGAGGAACGCATGTCACGTATCAAAGAAGATCCTTCTTTGAAGCCTATTTTGGAAGAAATAGAAACTGGTGGTCCAGCTGCTATGATGAGATACTGGAACGATAAAGATGTTTTGCAGAAGTTGGGAGAAGCAATGGGTCTTGCAGTTGGGGATGCAGCTACTTCTGCTGAACCACCAGAAGAAGCTGAAGATGCCGGAAATGAAGATGAATCAATTGTTCATCAAACTGCTAGTGTTGGTGATGTGGAGGGTTTGAAAGCTGCCCTAGCCTCTGGTGCTGACAAGGATGAGGAAGATTCAGAAGGGAGGACAGCATTACATTTTGCATGCGGATATGGTGAGGTGAAGTGTGCTCAAGTTCTTCTCGAGGCTGGAGCAAGAGTCGATGCTCTTGATAAGAACAAAAACACCGCACTTCATTATGCAGCCGGTTATGGCAGGAAGGAATGCGTGGCACTTCTACTTGAGAATGGCGCAGCTGTCACTCTCCAGAACATGGATGGCAAGACTCCGATCGACGTTGCCAAGCTGAACAACCAAAACGACGTACTAAAGTTGCTCGAGAAGGACGCCTTCCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005048 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006355 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008289 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009889 GO:0009941 GO:0010468 GO:0010556 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0019219 GO:0019222 GO:0019867 GO:0030941 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031323 GO:0031326 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0042170 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0051171 GO:0051179 GO:0051234 GO:0051252 GO:0051641 GO:0051649 GO:0051861 GO:0060255 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072594 GO:0072596 GO:0072598 GO:0080090 GO:0097367 GO:0098588 GO:0098805 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_3 Ank_4

Protein Analysis

363

Amino Acids

39.31

Weight (kDa)

4.37

Isoelectric Point (pI)

41.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STI1-HOP_DP	PF17830	172 - 215	1e-08	STI1/HOP, DP domain
Ank_KRIT1	PF24521	237 - 351	5.4e-06	KRIT1 ankyrin-repeats domain
Ank_2	PF12796	243 - 333	8e-20	Ankyrin repeats (3 copies)
Ank	PF00023	271 - 302	1.5e-06	Ankyrin repeat
Ank_4	PF13637	278 - 324	6e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	290 - 344	1.4e-10	Ankyrin repeats (many copies)
Ank_2	PF12796	298 - 357	3.1e-10	Ankyrin repeats (3 copies)
Ank	PF00023	303 - 334	1e-06	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007271)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17390 AT4G35450 AT4G35450 AT4G35450 AT4G35450 AT4G35450
fragaria_vesca	FvH4_2g17270 FvH4_2g17270
malus_domestica	MD05G1151600.v1.1 MD10G1145800.v1.1
prunus_persica	Prupe.8G184200_v2.0.a1
pyrus_communis	pycom05g14610 pycom05g14650 pycom10g12580
rosa_chinensis	RchiOBHm_Chr6g0281771
rosa_laevigata	RLG00000012936
rosa_multiflora	Rmu_ssc0000126.1_g000046
rosa_roxburghii	Rroxscaffold_7G00186810
rosa_rugosa	Rorug06G0143600 Rorug06G0143600
rosa_samantha	Rh6AG256300 Rh6BG259200 Rh6CG258200 Rh6DG250300
rosa_wichuraiana	Rw6G022220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	182, 1003
AciI	CCGC	2 cut(s)	837, 918
AclWI	GGATC	5 cut(s)	230, 269, 443, 456, 530
AcoI	YGGCCR	1 cut(s)	417
AcuI	CTGAAG	2 cut(s)	290, 714
AcyI	GRCGYC	1 cut(s)	1077
AfaI	GTAC	2 cut(s)	41, 1056
AfiI	CCNNNNNNNGG	2 cut(s)	182, 1003
AgsI	TTSAA	5 cut(s)	111, 286, 320, 547, 763
AhdI	GACNNNNNGTC	1 cut(s)	139
AjnI	CCWGG	1 cut(s)	151
AjuI	GAANNNNNNNTTGG	2 cut(s)	539, 571
AloI	GAACNNNNNNTCC	2 cut(s)	62, 94
Alw21I	GWGCWC	1 cut(s)	862
Alw26I	GTCTC	1 cut(s)	126
AlwI	GGATC	5 cut(s)	230, 269, 443, 456, 530
Ama87I	CYCGRG	2 cut(s)	872, 1067
AoxI	GGCC	1 cut(s)	417
ApeKI	GCWGC	7 cut(s)	10, 13, 584, 662, 767, 932, 983
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	2 cut(s)	426, 983
AspS9I	GGNCC	1 cut(s)	578
AsuHPI	GGTGA	3 cut(s)	758, 857, 862
AvaI	CYCGRG	2 cut(s)	872, 1067
AvaII	GGWCC	1 cut(s)	578
BamHI	GGATCC	1 cut(s)	448
Bbv12I	GWGCWC	1 cut(s)	862
BbvI	GCAGC	5 cut(s)	571, 674, 754, 944, 995
BccI	CCATC	4 cut(s)	286, 460, 496, 1001
BceAI	ACGGC	1 cut(s)	61
BciT130I	CCWGG	1 cut(s)	153
BcoDI	GTCTC	1 cut(s)	126
BfaI	CTAG	4 cut(s)	240, 254, 738, 773
BfoI	RGCGCY	1 cut(s)	427
BisI	GCNGC	7 cut(s)	11, 14, 585, 663, 768, 933, 984
BlsI	GCNGC	7 cut(s)	12, 15, 586, 664, 769, 934, 985
Bme1390I	CCNGG	1 cut(s)	153
Bme18I	GGWCC	1 cut(s)	578
BmeRI	GACNNNNNGTC	1 cut(s)	139
BmeT110I	CYCGRG	2 cut(s)	872, 1067
BmgT120I	GGNCC	1 cut(s)	578
BmiI	GGNNCC	1 cut(s)	450
BmrFI	CCNGG	1 cut(s)	153
BmsI	GCATC	5 cut(s)	131, 432, 649, 688, 883
BpmI	CTGGAG	4 cut(s)	38, 150, 900, 980
BpuEI	CTTGAG	4 cut(s)	491, 527, 846, 992
BsaAI	YACGTR	1 cut(s)	524
BsaHI	GRCGYC	1 cut(s)	1077
BsaJI	CCNNGG	3 cut(s)	152, 414, 427
BsaXI	ACNNNNNCTCC	4 cut(s)	62, 92, 873, 903
Bsc4I	CCNNNNNNNGG	2 cut(s)	182, 1003
Bse118I	RCCGGY	1 cut(s)	935
Bse1I	ACTGG	4 cut(s)	56, 226, 577, 605
Bse3DI	GCAATG	1 cut(s)	645
BseBI	CCWGG	1 cut(s)	153
BseDI	CCNNGG	3 cut(s)	152, 414, 427
BseGI	GGATG	4 cut(s)	42, 664, 799, 1012
BseLI	CCNNNNNNNGG	2 cut(s)	182, 1003
BseMI	GCAATG	1 cut(s)	645
BseMII	CTCAG	4 cut(s)	108, 225, 362, 416
BseNI	ACTGG	4 cut(s)	56, 226, 577, 605
BseXI	GCAGC	5 cut(s)	571, 674, 754, 944, 995
Bsh1285I	CGRYCG	1 cut(s)	1023
BshFI	GGCC	1 cut(s)	419
BsiEI	CGRYCG	1 cut(s)	1023
BsiHKAI	GWGCWC	1 cut(s)	862
BsiHKCI	CYCGRG	2 cut(s)	872, 1067
BsiSI	CCGG	2 cut(s)	702, 936
BslFI	GGGAC	1 cut(s)	329
BslI	CCNNNNNNNGG	2 cut(s)	182, 1003
BsmAI	GTCTC	1 cut(s)	126
BsmFI	GGGAC	1 cut(s)	329
BsmI	GAATGC	1 cut(s)	959
BsnI	GGCC	1 cut(s)	419
BsoBI	CYCGRG	2 cut(s)	872, 1067
Bsp1286I	GDGCHC	1 cut(s)	862
Bsp143I	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
Bsp19I	CCATGG	1 cut(s)	414
BspACI	CCGC	2 cut(s)	837, 918
BspANI	GGCC	1 cut(s)	419
BspCNI	CTCAG	4 cut(s)	109, 224, 361, 415
BspHI	TCATGA	1 cut(s)	408
BspLI	GGNNCC	1 cut(s)	450
BspPI	GGATC	5 cut(s)	230, 269, 443, 456, 530
BsrDI	GCAATG	1 cut(s)	645
BsrFI	RCCGGY	1 cut(s)	935
BsrI	ACTGG	4 cut(s)	56, 226, 577, 605
BssAI	RCCGGY	1 cut(s)	935
BssECI	CCNNGG	3 cut(s)	152, 414, 427
BssMI	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
BssNI	GRCGYC	1 cut(s)	1077
BssT1I	CCWWGG	1 cut(s)	414
Bst2UI	CCWGG	1 cut(s)	153
Bst4CI	ACNGT	1 cut(s)	141
BstACI	GRCGYC	1 cut(s)	1077
BstBAI	YACGTR	1 cut(s)	524
BstC8I	GCNNGC	2 cut(s)	227, 835
BstDEI	CTNAG	4 cut(s)	117, 211, 348, 402
BstDSI	CCRYGG	1 cut(s)	414
BstF5I	GGATG	4 cut(s)	42, 664, 799, 1012
BstH2I	RGCGCY	1 cut(s)	427
BstHHI	GCGC	2 cut(s)	426, 983
BstKTI	GATC	6 cut(s)	238, 277, 451, 502, 538, 1023
BstMAI	GTCTC	1 cut(s)	126
BstMBI	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
BstMCI	CGRYCG	1 cut(s)	1023
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 19, 671, 698, 773, 782
BstNI	CCWGG	1 cut(s)	153
BstNSI	RCATGY	2 cut(s)	520, 837
BstSCI	CCNGG	1 cut(s)	151
BstV1I	GCAGC	5 cut(s)	571, 674, 754, 944, 995
BstX2I	RGATCY	3 cut(s)	274, 448, 535
BstYI	RGATCY	3 cut(s)	274, 448, 535
BsuRI	GGCC	1 cut(s)	419
BtgI	CCRYGG	1 cut(s)	414
BtsCI	GGATG	4 cut(s)	42, 664, 799, 1012
BtsIMutI	CAGTG	1 cut(s)	137
Cac8I	GCNNGC	2 cut(s)	227, 835
CciI	TCATGA	1 cut(s)	408
CfoI	GCGC	2 cut(s)	426, 983
Cfr10I	RCCGGY	1 cut(s)	935
Cfr13I	GGNCC	1 cut(s)	578
Csp6I	GTAC	2 cut(s)	40, 1055
CviAII	CATG	8 cut(s)	217, 379, 409, 415, 466, 517, 834, 1003
CviQI	GTAC	2 cut(s)	40, 1055
DdeI	CTNAG	4 cut(s)	117, 211, 348, 402
DpnI	GATC	6 cut(s)	237, 276, 450, 501, 537, 1022
DpnII	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
DriI	GACNNNNNGTC	1 cut(s)	139
EaeI	YGGCCR	1 cut(s)	417
Eam1105I	GACNNNNNGTC	1 cut(s)	139
Eco130I	CCWWGG	1 cut(s)	414
Eco32I	GATATC	1 cut(s)	267
Eco47I	GGWCC	1 cut(s)	578
Eco57I	CTGAAG	2 cut(s)	290, 714
Eco88I	CYCGRG	2 cut(s)	872, 1067
EcoRII	CCWGG	1 cut(s)	151
EcoRV	GATATC	1 cut(s)	267
EcoT14I	CCWWGG	1 cut(s)	414
EcoT22I	ATGCAT	1 cut(s)	439
ErhI	CCWWGG	1 cut(s)	414
FaeI	CATG	8 cut(s)	220, 382, 412, 418, 469, 520, 837, 1006
FalI	AAGNNNNNCTT	3 cut(s)	489, 521, 1064
FaqI	GGGAC	1 cut(s)	329
FatI	CATG	8 cut(s)	216, 378, 408, 414, 465, 516, 833, 1002
Fnu4HI	GCNGC	7 cut(s)	11, 14, 585, 663, 768, 933, 984
FokI	GGATG	4 cut(s)	49, 671, 806, 1019
Fsp4HI	GCNGC	7 cut(s)	11, 14, 585, 663, 768, 933, 984
FspBI	CTAG	4 cut(s)	240, 254, 738, 773
GlaI	GCGC	2 cut(s)	425, 982
GluI	GCNGC	7 cut(s)	11, 14, 585, 663, 768, 933, 984
GsuI	CTGGAG	4 cut(s)	38, 150, 900, 980
HaeII	RGCGCY	1 cut(s)	427
HaeIII	GGCC	1 cut(s)	419
HapII	CCGG	2 cut(s)	702, 936
HhaI	GCGC	2 cut(s)	426, 983
Hin1I	GRCGYC	1 cut(s)	1077
Hin1II	CATG	8 cut(s)	220, 382, 412, 418, 469, 520, 837, 1006
Hin6I	GCGC	2 cut(s)	424, 981
HinP1I	GCGC	2 cut(s)	424, 981
HinfI	GANTC	6 cut(s)	134, 397, 716, 803, 888, 1015
HpaII	CCGG	2 cut(s)	702, 936
HphI	GGTGA	3 cut(s)	758, 857, 862
Hpy188I	TCNGA	5 cut(s)	118, 291, 309, 808, 1020
Hpy188III	TCNNGA	8 cut(s)	129, 144, 176, 409, 872, 899, 997, 1069
Hpy99I	CGWCG	3 cut(s)	47, 1028, 1055
HpyAV	CCTTC	6 cut(s)	288, 332, 549, 803, 943, 1066
HpyCH4III	ACNGT	1 cut(s)	141
HpyCH4IV	ACGT	3 cut(s)	523, 1026, 1053
HpyCH4V	TGCA	9 cut(s)	382, 394, 437, 445, 622, 650, 662, 833, 932
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 19, 671, 698, 773, 782
HpyF3I	CTNAG	4 cut(s)	117, 211, 348, 402
HpySE526I	ACGT	3 cut(s)	523, 1026, 1053
Hsp92I	GRCGYC	1 cut(s)	1077
Hsp92II	CATG	8 cut(s)	220, 382, 412, 418, 469, 520, 837, 1006
HspAI	GCGC	2 cut(s)	424, 981
Kzo9I	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
LmnI	GCTCC	4 cut(s)	9, 19, 83, 881
Lsp1109I	GCAGC	5 cut(s)	571, 674, 754, 944, 995
LweI	GCATC	5 cut(s)	131, 432, 649, 688, 883
MaeI	CTAG	4 cut(s)	240, 254, 738, 773
MaeII	ACGT	3 cut(s)	523, 1026, 1053
MaeIII	GTNAC	2 cut(s)	519, 988
MalI	GATC	6 cut(s)	237, 276, 450, 501, 537, 1022
MboI	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
MboII	GAAGA	7 cut(s)	545, 572, 698, 707, 722, 812, 860
MfeI	CAATTG	1 cut(s)	720
MflI	RGATCY	3 cut(s)	274, 448, 535
MhlI	GDGCHC	1 cut(s)	862
MluCI	AATT	1 cut(s)	720
MlyI	GAGTC	3 cut(s)	128, 897, 1009
MmeI	TCCRAC	2 cut(s)	86, 193
Mph1103I	ATGCAT	1 cut(s)	439
MroXI	GAANNNNTTC	1 cut(s)	303
MslI	CAYNNNNRTG	1 cut(s)	413
MspA1I	CMGCKG	2 cut(s)	584, 986
MspI	CCGG	2 cut(s)	702, 936
MspR9I	CCNGG	1 cut(s)	153
MunI	CAATTG	1 cut(s)	720
Mva1269I	GAATGC	1 cut(s)	959
MvaI	CCWGG	1 cut(s)	153
MwoI	GCNNNNNNNGC	6 cut(s)	10, 19, 671, 698, 773, 782
NcoI	CCATGG	1 cut(s)	414
NdeII	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
NlaIII	CATG	8 cut(s)	220, 382, 412, 418, 469, 520, 837, 1006
NlaIV	GGNNCC	1 cut(s)	450
NmeAIII	GCCGAG	1 cut(s)	445
NmuCI	GTSAC	2 cut(s)	519, 988
NsiI	ATGCAT	1 cut(s)	439
NspI	RCATGY	2 cut(s)	520, 837
PaeI	GCATGC	1 cut(s)	837
PaeR7I	CTCGAG	2 cut(s)	872, 1067
PagI	TCATGA	1 cut(s)	408
PcsI	WCGNNNNNNNCGW	1 cut(s)	520
PctI	GAATGC	1 cut(s)	959
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	3 cut(s)	397, 716, 803
PflMI	CCANNNNNTGG	2 cut(s)	182, 1003
PkrI	GCNGC	7 cut(s)	12, 15, 586, 664, 769, 934, 985
Ple19I	CGATCG	1 cut(s)	1023
PleI	GAGTC	3 cut(s)	128, 896, 1009
PpsI	GAGTC	3 cut(s)	128, 896, 1009
Ppu21I	YACGTR	1 cut(s)	524
Psp6I	CCWGG	1 cut(s)	151
PspGI	CCWGG	1 cut(s)	151
PspN4I	GGNNCC	1 cut(s)	450
PspPI	GGNCC	1 cut(s)	578
PsuI	RGATCY	3 cut(s)	274, 448, 535
PvuI	CGATCG	1 cut(s)	1023
PvuII	CAGCTG	2 cut(s)	584, 986
RsaI	GTAC	2 cut(s)	41, 1056
RsaNI	GTAC	2 cut(s)	40, 1055
RseI	CAYNNNNRTG	1 cut(s)	413
SatI	GCNGC	7 cut(s)	11, 14, 585, 663, 768, 933, 984
Sau3AI	GATC	6 cut(s)	235, 274, 448, 499, 535, 1020
Sau96I	GGNCC	1 cut(s)	578
SchI	GAGTC	3 cut(s)	128, 897, 1009
ScrFI	CCNGG	1 cut(s)	153
SduI	GDGCHC	1 cut(s)	862
SfaNI	GCATC	5 cut(s)	131, 432, 649, 688, 883
Sfr274I	CTCGAG	2 cut(s)	872, 1067
SinI	GGWCC	1 cut(s)	578
SlaI	CTCGAG	2 cut(s)	872, 1067
SmiMI	CAYNNNNRTG	1 cut(s)	413
SmlI	CTYRAG	6 cut(s)	470, 506, 861, 872, 971, 1067
SmoI	CTYRAG	6 cut(s)	470, 506, 861, 872, 971, 1067
SphI	GCATGC	1 cut(s)	837
Sse9I	AATT	1 cut(s)	720
SsiI	CCGC	2 cut(s)	837, 918
SspMI	CTAG	4 cut(s)	240, 254, 738, 773
StyD4I	CCNGG	1 cut(s)	151
StyI	CCWWGG	1 cut(s)	414
TaaI	ACNGT	1 cut(s)	141
TaiI	ACGT	3 cut(s)	526, 1029, 1056
TaqI	TCGA	4 cut(s)	873, 891, 1023, 1068
TasI	AATT	1 cut(s)	720
TfiI	GAWTC	3 cut(s)	397, 716, 803
TscAI	CASTG	1 cut(s)	144
TseFI	GTSAC	2 cut(s)	519, 988
TseI	GCWGC	7 cut(s)	10, 13, 584, 662, 767, 932, 983
Tsp45I	GTSAC	2 cut(s)	519, 988
TspDTI	ATGAA	8 cut(s)	270, 351, 397, 473, 716, 723, 729, 914
TspRI	CASTG	1 cut(s)	144
Van91I	CCANNNNNTGG	2 cut(s)	182, 1003
VpaK11BI	GGWCC	1 cut(s)	578
XceI	RCATGY	2 cut(s)	520, 837
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1004
XhoI	CTCGAG	2 cut(s)	872, 1067
XmnI	GAANNNNTTC	1 cut(s)	303
XspI	CTAG	4 cut(s)	240, 254, 738, 773
Zsp2I	ATGCAT	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.