MD05G1156300.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011730
Physical Location & Seq
Forward (+)
28625059 .. 28627308
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1156300.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGATGTAGGTACTTTGGGAGGTCTGGTTCCGGAACCTTATGTGGGTATGGAATTTGATTCCGAAGATGCTGCCAGGAAATTCTATACTGACTATGCCAGACAGGTCGGGTTTGTTGTCCGTGTTATGCAGCGTCGTCGATCAGGAATTGATGGGAGAACTCTTGCCCGTCGGCTTGGGTGTAACAAACAAGGGTTTTCTCCTAACCAGCCGGGAAAATTTGGACCTGAGAAAAAGCCTAGACCTAGTGCACGAGAAGGTTGTAAGGCAACAATCTTGGTGAAGATGGAAAAATCTGGAAAATGGGTTGTTACAAGATTTGTAAAGGATCATAATCATCCTCTGGTCGTTACTGCCAATGGTTTTAGCACAGAGGGTGATAAGGATCGGAAAATTGAGGAACTTACACTGGAGTTGGAGCATCAGGATCAACGATGTGCAGCTTATCGAGAAAAATTACTAAGCTTCATGCACAATGTTGAGGCGGAAACAGAAGAACTGTCTGGAAAGATACAAGTTATTGTCGAAAATGTGAGAAAAGTTGAATCTGAAATGCAAAAACATTCCTTTCGTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

193

Amino Acids

21.91

Weight (kDa)

9.25

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 27 - 116 1.7e-29 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 1 cut(s) 485
AclWI GGATC 3 cut(s) 336, 393, 435
AcsI RAATTY 3 cut(s) 53, 80, 218
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 1 cut(s) 545
AjnI CCWGG 1 cut(s) 74
AloI GAACNNNNNNTCC 2 cut(s) 12, 44
AluBI AGCT 2 cut(s) 443, 465
AluI AGCT 2 cut(s) 443, 465
Alw21I GWGCWC 1 cut(s) 253
Alw44I GTGCAC 1 cut(s) 249
AlwI GGATC 3 cut(s) 336, 393, 435
Aor13HI TCCGGA 1 cut(s) 31
ApaLI GTGCAC 1 cut(s) 249
ApeKI GCWGC 3 cut(s) 71, 130, 440
ApoI RAATTY 3 cut(s) 53, 80, 218
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 292, 389
AvaII GGWCC 1 cut(s) 224
BaeGI GKGCMC 1 cut(s) 253
BauI CACGAG 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 3 cut(s) 58, 142, 452
BccI CCATC 2 cut(s) 146, 280
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 119, 153
BciT130I CCWGG 1 cut(s) 76
BcnI CCSGG 1 cut(s) 213
BfaI CTAG 2 cut(s) 240, 246
BisI GCNGC 3 cut(s) 72, 131, 441
BlsI GCNGC 3 cut(s) 73, 132, 442
Bme1390I CCNGG 2 cut(s) 76, 213
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 2 cut(s) 30, 36
BmrFI CCNGG 2 cut(s) 76, 213
BmsI GCATC 2 cut(s) 58, 430
BpmI CTGGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 213
BsaBI GATNNNNATC 2 cut(s) 333, 384
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 414
Bse8I GATNNNNATC 2 cut(s) 333, 384
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 1 cut(s) 76
BseGI GGATG 2 cut(s) 10, 337
BseJI GATNNNNATC 2 cut(s) 333, 384
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 414
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 3 cut(s) 58, 142, 452
BsgI GTGCAG 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 2 cut(s) 32, 212
BslI CCNNNNNNNGG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 253
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 4 cut(s) 140, 328, 385, 427
BspACI CCGC 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 30, 36
BspPI GGATC 3 cut(s) 336, 393, 435
BsrI ACTGG 1 cut(s) 414
BssMI GATC 4 cut(s) 140, 328, 385, 427
BssSI CACGAG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 1 cut(s) 501
BstDEI CTNAG 2 cut(s) 228, 461
BstF5I GGATG 2 cut(s) 10, 337
BstKTI GATC 4 cut(s) 143, 331, 388, 430
BstMBI GATC 4 cut(s) 140, 328, 385, 427
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 74, 211
BstSLI GKGCMC 1 cut(s) 253
BstV1I GCAGC 3 cut(s) 58, 142, 452
BtsCI GGATG 2 cut(s) 10, 337
BtsIMutI CAGTG 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 12
CviAII CATG 1 cut(s) 469
CviJI RGCY 5 cut(s) 175, 211, 238, 443, 465
CviKI_1 RGCY 5 cut(s) 175, 211, 238, 443, 465
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 228, 461
DpnI GATC 4 cut(s) 142, 330, 387, 429
DpnII GATC 4 cut(s) 140, 328, 385, 427
EciI GGCGGA 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 74
FaeI CATG 1 cut(s) 472
FaiI YATR 7 cut(s) 42, 50, 87, 96, 128, 333, 470
FatI CATG 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 72, 131, 441
FokI GGATG 2 cut(s) 17, 324
Fsp4HI GCNGC 3 cut(s) 72, 131, 441
FspBI CTAG 2 cut(s) 240, 246
GluI GCNGC 3 cut(s) 72, 131, 441
GsuI CTGGAG 1 cut(s) 431
HapII CCGG 2 cut(s) 32, 212
HgaI GACGC 1 cut(s) 122
Hin1II CATG 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 2 cut(s) 59, 545
HpaII CCGG 2 cut(s) 32, 212
HphI GGTGA 2 cut(s) 292, 389
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 3 cut(s) 64, 390, 550
Hpy188III TCNNGA 6 cut(s) 32, 144, 297, 425, 449, 504
Hpy8I GTNNAC 1 cut(s) 251
Hpy99I CGWCG 3 cut(s) 138, 141, 174
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 501
HpyCH4V TGCA 5 cut(s) 130, 251, 440, 472, 556
HpyF3I CTNAG 2 cut(s) 228, 461
Hsp92II CATG 1 cut(s) 472
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 4 cut(s) 140, 328, 385, 427
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 3 cut(s) 58, 142, 452
LweI GCATC 2 cut(s) 58, 430
MaeI CTAG 2 cut(s) 240, 246
MaeIII GTNAC 3 cut(s) 182, 310, 349
MalI GATC 4 cut(s) 142, 330, 387, 429
MboI GATC 4 cut(s) 140, 328, 385, 427
MboII GAAGA 3 cut(s) 77, 295, 506
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 6 cut(s) 53, 80, 147, 218, 393, 455
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 5 cut(s) 14, 351, 367, 391, 475
MroI TCCGGA 1 cut(s) 31
MspI CCGG 2 cut(s) 32, 212
MspR9I CCNGG 2 cut(s) 76, 213
MvaI CCWGG 1 cut(s) 76
NciI CCSGG 1 cut(s) 213
NdeII GATC 4 cut(s) 140, 328, 385, 427
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 30, 36
PfeI GAWTC 2 cut(s) 59, 545
PkrI GCNGC 3 cut(s) 73, 132, 442
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 30, 36
PspPI GGNCC 1 cut(s) 224
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SatI GCNGC 3 cut(s) 72, 131, 441
Sau3AI GATC 4 cut(s) 140, 328, 385, 427
Sau96I GGNCC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 76, 213
SduI GDGCHC 1 cut(s) 253
SetI ASST 9 cut(s) 13, 25, 40, 108, 229, 247, 262, 445, 467
SfaNI GCATC 2 cut(s) 58, 430
SinI GGWCC 1 cut(s) 224
Sse9I AATT 6 cut(s) 53, 80, 147, 218, 393, 455
SsiI CCGC 1 cut(s) 485
SspMI CTAG 2 cut(s) 240, 246
StyD4I CCNGG 2 cut(s) 74, 211
TaaI ACNGT 1 cut(s) 501
TaqI TCGA 3 cut(s) 139, 448, 525
TasI AATT 6 cut(s) 53, 80, 147, 218, 393, 455
TfiI GAWTC 2 cut(s) 59, 545
TscAI CASTG 1 cut(s) 414
TseI GCWGC 3 cut(s) 71, 130, 440
TspDTI ATGAA 1 cut(s) 457
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 414
VneI GTGCAC 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 3 cut(s) 53, 80, 218
XspI CTAG 2 cut(s) 240, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.