MD05G1156400.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011730
Physical Location & Seq
Reverse (-)
28627520 .. 28628582
1063 bp
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UTR
Exon/CDS
Intron
MD05G1156400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGAGAAGAGAATGTTGTAAAGCAATGATGCTAGTCAAACTTGATGAGTCTGGAAAATGGGTTGTTACAAGAGTTGTTAAGGACCATGCTCATCCACTTATAGTTTCTTCCGGGAATTCTATGGACGCAAAGGATATGAAGATTGCGGAACTCACTATGGACCTGGGGCGTGAAGAGCAATTATGTGGACAATACCATGAGCCACTACTTACATTGCTGAATAATGTTGAGGCGGAGACGGAACTGATAGCAACGAAAGTTAGAGGAGTCGTTAACCATGTAAGAGAATTTGAGACTGACGTTGAAGGGCTTCCACAAAATCAAATGGAGGCTAAAGTTCGACGGCTTCCTCCAAATCAAACGGTGACTGAAGTTCAAGGGCTTCCACAAAGTCAATCAGGCCACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

137

Amino Acids

15.3

Weight (kDa)

5.72

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 3 - 34 5.7e-08 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 146, 233
AcsI RAATTY 2 cut(s) 115, 287
AcuI CTGAAG 1 cut(s) 390
AgsI TTSAA 2 cut(s) 305, 377
AjnI CCWGG 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 230, 287
AoxI GGCC 1 cut(s) 400
ApoI RAATTY 2 cut(s) 115, 287
Asp700I GAANNNNTTC 1 cut(s) 309
AspS9I GGNCC 2 cut(s) 82, 160
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 2 cut(s) 82, 160
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 230, 287
BfaI CTAG 1 cut(s) 32
Bme1390I CCNGG 2 cut(s) 112, 164
Bme18I GGWCC 2 cut(s) 82, 160
BmgT120I GGNCC 2 cut(s) 82, 160
BmrFI CCNGG 2 cut(s) 112, 164
BmsI GCATC 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 163
Bse3DI GCAATG 2 cut(s) 30, 212
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 91
BseMI GCAATG 2 cut(s) 30, 212
BseRI GAGGAG 1 cut(s) 279
BshFI GGCC 1 cut(s) 402
BsiSI CCGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 230, 287
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 1 cut(s) 402
BspACI CCGC 2 cut(s) 146, 233
BspANI GGCC 1 cut(s) 402
BspQI GCTCTTC 1 cut(s) 168
BsrDI GCAATG 2 cut(s) 30, 212
BssECI CCNNGG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 168
BstF5I GGATG 1 cut(s) 91
BstMAI GTCTC 2 cut(s) 230, 287
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 110, 162
BsuRI GGCC 1 cut(s) 402
BtsCI GGATG 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 82, 160
CseI GACGC 1 cut(s) 134
CviAII CATG 3 cut(s) 86, 197, 278
CviJI RGCY 6 cut(s) 202, 310, 332, 346, 382, 402
CviKI_1 RGCY 6 cut(s) 202, 310, 332, 346, 382, 402
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 82, 160
Eco57I CTGAAG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 3 cut(s) 89, 200, 281
FaiI YATR 8 cut(s) 87, 101, 122, 137, 158, 184, 198, 279
FatI CATG 3 cut(s) 85, 196, 277
FokI GGATG 1 cut(s) 78
FspBI CTAG 1 cut(s) 32
HaeIII GGCC 1 cut(s) 402
HapII CCGG 1 cut(s) 111
HgaI GACGC 1 cut(s) 134
Hin1II CATG 3 cut(s) 89, 200, 281
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 47, 267
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 2 cut(s) 188, 274
Hpy188III TCNNGA 1 cut(s) 51
Hpy8I GTNNAC 2 cut(s) 188, 274
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 3 cut(s) 89, 200, 281
KspAI GTTAAC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 168
LpnPI CCDG 5 cut(s) 36, 124, 149, 176, 384
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 64, 364
MboII GAAGA 4 cut(s) 18, 99, 151, 185
MluCI AATT 4 cut(s) 115, 179, 287, 406
MlyI GAGTC 2 cut(s) 56, 276
MnlI CCTC 4 cut(s) 223, 257, 322, 360
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 2 cut(s) 78, 273
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 112, 164
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 112
NlaIII CATG 3 cut(s) 89, 200, 281
NmuCI GTSAC 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 110
PleI GAGTC 2 cut(s) 55, 275
PpsI GAGTC 2 cut(s) 55, 275
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspPI GGNCC 2 cut(s) 82, 160
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 78, 273
Sau96I GGNCC 2 cut(s) 82, 160
SchI GAGTC 2 cut(s) 56, 276
ScrFI CCNGG 2 cut(s) 112, 164
SetI ASST 2 cut(s) 165, 303
SfaNI GCATC 1 cut(s) 18
SinI GGWCC 2 cut(s) 82, 160
Sse9I AATT 4 cut(s) 115, 179, 287, 406
SsiI CCGC 2 cut(s) 146, 233
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 110, 162
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 340
TasI AATT 4 cut(s) 115, 179, 287, 406
Tru1I TTAA 2 cut(s) 78, 273
Tru9I TTAA 2 cut(s) 78, 273
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 82, 160
XapI RAATTY 2 cut(s) 115, 287
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.