MD10G1150000.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011730
Physical Location & Seq
Reverse (-)
23717986 .. 23720076
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1150000.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGAGTTTGAGTCTGAAGATGCTGCCAAGGAGTTTTACGAAGATTATGCAAGGCGTGTTGGATTCATCATGCGTATAGGCCAGTGTCGTCGATCACATGTTGAGAAGAGAATTCTTTCCCGTAAGCTATCCTGCAATAAGCAAGGTGGGCGTTCTTTAAAAGCTAGAGACCAAGTCCGGTCAGTTCGAAGACCGCAACCTACAACAGGAGAAGGGTGTAAAGCGATGATGCTAGTCAAACTTAAAAAATCTGGAAAATGGGTCATTACAAGAGTTGTCAAGGACCATACTCATCCACTTATAGTTTCTTCCGGGAATTCTATGGATGCAAAGGATATGAAGATTGCAGAACTCACTATGGACCTGGAGCGTGAAGAGCAATTATGTGGACAATATCGTGAGCTACTACTTGCATTGCTGAATAATGTTGAGGAGGAGACAGACCACATAGCAACGAAAGTTAGAGGAGTCGTAAACTATGTAAGAGAATTTGAGACTCAAGTTGAAGGGATTCCAGAAAATCAAATGGAGTCAAGTTCGAGGGCTTCCTCAAAATCAAACGGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

190

Amino Acids

21.59

Weight (kDa)

8.87

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 11 - 101 1.2e-25 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 194
AcsI RAATTY 3 cut(s) 111, 316, 488
AcuI CTGAAG 1 cut(s) 36
AfiI CCNNNNNNNGG 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 1 cut(s) 506
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 3 cut(s) 127, 164, 403
AluI AGCT 3 cut(s) 127, 164, 403
Alw26I GTCTC 4 cut(s) 162, 431, 488, 558
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 3 cut(s) 111, 316, 488
Asp700I GAANNNNTTC 2 cut(s) 115, 510
AspS9I GGNCC 2 cut(s) 283, 361
AsuC2I CCSGG 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 187
AvaII GGWCC 2 cut(s) 283, 361
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 10
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 1 cut(s) 365
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 4 cut(s) 162, 431, 488, 558
BfaI CTAG 2 cut(s) 165, 233
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 313, 365
Bme18I GGWCC 2 cut(s) 283, 361
BmgT120I GGNCC 2 cut(s) 283, 361
BmrFI CCNGG 2 cut(s) 313, 365
BmsI GCATC 3 cut(s) 10, 219, 316
BpiI GAAGAC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 187
BpuEI CTTGAG 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 413
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 292, 331
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 413
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 3 cut(s) 446, 449, 480
BseXI GCAGC 1 cut(s) 10
BshFI GGCC 1 cut(s) 81
BsiSI CCGG 2 cut(s) 178, 312
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 4 cut(s) 162, 431, 488, 558
BsnI GGCC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 187
Bsp143I GATC 1 cut(s) 92
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 413
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 365
Bst6I CTCTTC 2 cut(s) 101, 369
BstBI TTCGAA 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 292, 331
BstKTI GATC 1 cut(s) 95
BstMAI GTCTC 4 cut(s) 162, 431, 488, 558
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 148, 376
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 311, 363
BstV1I GCAGC 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 196
BsuRI GGCC 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 292, 331
BtsIMutI CAGTG 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 283, 361
CviAII CATG 2 cut(s) 70, 98
CviJI RGCY 5 cut(s) 81, 127, 164, 403, 545
CviKI_1 RGCY 5 cut(s) 81, 127, 164, 403, 545
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
DraI TTTAAA 1 cut(s) 159
Eam1104I CTCTTC 2 cut(s) 101, 369
EarI CTCTTC 2 cut(s) 101, 369
Eco130I CCWWGG 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 2 cut(s) 283, 361
Eco57I CTGAAG 1 cut(s) 36
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoRI GAATTC 2 cut(s) 111, 316
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 73, 101
FatI CATG 2 cut(s) 69, 97
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 2 cut(s) 279, 338
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 2 cut(s) 165, 233
GluI GCNGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 1 cut(s) 81
HapII CCGG 2 cut(s) 178, 312
Hin1II CATG 2 cut(s) 73, 101
HinfI GANTC 6 cut(s) 11, 63, 468, 496, 511, 530
HpaII CCGG 2 cut(s) 178, 312
Hpy166II GTNNAC 2 cut(s) 389, 475
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 3 cut(s) 252, 398, 515
Hpy8I GTNNAC 2 cut(s) 389, 475
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 206, 500
HpyCH4V TGCA 5 cut(s) 50, 135, 329, 347, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 376
Hsp92II CATG 2 cut(s) 73, 101
Kzo9I GATC 1 cut(s) 92
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 9 cut(s) 95, 145, 191, 192, 237, 325, 350, 377, 528
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 3 cut(s) 10, 219, 316
MaeI CTAG 2 cut(s) 165, 233
MalI GATC 1 cut(s) 94
MboI GATC 1 cut(s) 92
MboII GAAGA 7 cut(s) 29, 53, 118, 201, 300, 352, 386
MluCI AATT 4 cut(s) 111, 316, 380, 488
MlyI GAGTC 4 cut(s) 20, 477, 490, 539
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 5 cut(s) 424, 427, 458, 534, 559
MroXI GAANNNNTTC 2 cut(s) 115, 510
MseI TTAA 2 cut(s) 158, 243
MspI CCGG 2 cut(s) 178, 312
MspR9I CCNGG 2 cut(s) 313, 365
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 2 cut(s) 148, 376
NciI CCSGG 1 cut(s) 313
NdeII GATC 1 cut(s) 92
NlaIII CATG 2 cut(s) 73, 101
NspI RCATGY 1 cut(s) 101
NspV TTCGAA 1 cut(s) 187
PciI ACATGT 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 369
PdmI GAANNNNTTC 2 cut(s) 115, 510
PfeI GAWTC 2 cut(s) 63, 511
PflFI GACNNNGTC 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 311
PkrI GCNGC 1 cut(s) 25
PleI GAGTC 4 cut(s) 19, 476, 490, 538
PpsI GAGTC 4 cut(s) 19, 476, 490, 538
PscI ACATGT 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspPI GGNCC 2 cut(s) 283, 361
PsyI GACNNNGTC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 158, 243
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 2 cut(s) 283, 361
SchI GAGTC 4 cut(s) 20, 477, 490, 539
ScrFI CCNGG 2 cut(s) 313, 365
SetI ASST 6 cut(s) 129, 148, 166, 202, 366, 405
SfaNI GCATC 3 cut(s) 10, 219, 316
SfuI TTCGAA 1 cut(s) 187
SinI GGWCC 2 cut(s) 283, 361
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
Sse9I AATT 4 cut(s) 111, 316, 380, 488
SsiI CCGC 1 cut(s) 194
SspMI CTAG 2 cut(s) 165, 233
StyD4I CCNGG 2 cut(s) 311, 363
StyI CCWWGG 1 cut(s) 27
TaqI TCGA 3 cut(s) 91, 187, 539
TasI AATT 4 cut(s) 111, 316, 380, 488
TfiI GAWTC 2 cut(s) 63, 511
Tru1I TTAA 2 cut(s) 158, 243
Tru9I TTAA 2 cut(s) 158, 243
TscAI CASTG 1 cut(s) 89
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 55, 353
TspRI CASTG 1 cut(s) 89
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 283, 361
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 3 cut(s) 111, 316, 488
XceI RCATGY 1 cut(s) 101
XmnI GAANNNNTTC 2 cut(s) 115, 510
XspI CTAG 2 cut(s) 165, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.