MD05G1173200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014531
Physical Location & Seq
Forward (+)
30192261 .. 30193665
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1173200.v1.1.491

Sequence Viewer

Length: 624 bp
ATGTCAGAAAACAGAGTCAGGACGATAAAGCTGCGCTGCCCATCGGCTGCGAAAACGGCGTCATTCGCTGCACAGGAGGAGCAGAAGCTGGACCTGGGCTCCATAGCCAGAGCATTCGGGGTGGACCCACAAACGCTGAAGCTCAACGGACACTTCATCGGCAGAGGCTCCGACCTCATCGCTTCCTCGGTCACCTGGAAGTCCCTCCTCTCTTTCTTCTTCACCAAACGACTCCCCACGGGGGAAGACGACGCCAACCCCATCGTCGTCGACGGCAAGCTCTGTAGAGCTGGCACCAAGAGAGTTGGGTGTGACGTTGTTGATGATAGCAGAGGAAGAGAGGCAGAGTTGGAAGATGGGGAATTTTTGGGGATGAAGAGTAAAAAGATGAGGACGACTAGCAATGTTGGAATTTGCTCCAAGAGGAAGCAATTGTTGGAGGATGTGAAGTTGCTCAAAAAGTTGAAAATTAGTGAAACCAACCCAGATATTCAAGGAAGCAGTAATGGACTGTGCAAGAGCATCGCGAGCAACCACTTCAGTTGCAGTTATATGAGTGGGAGTATGAAGAGGATGACAGAAGATGAGGCAGGTGTGGCTTCTCCTTGCAAAAAAATTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.61

Weight (kDa)

9.36

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014531)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07150
fragaria_vesca FvH4_2g18740 FvH4_2g18740
malus_domestica MD05G1173200.v1.1
prunus_persica Prupe.8G200700_v2.0.a1
pyrus_communis pycom05g16000
rosa_chinensis RchiOBHm_Chr6g0284361
rosa_laevigata RLG00000012747
rosa_multiflora Rmu_sc0002698.1_g000002 Rmu_sc0018899.1_g000004
rosa_roxburghii Rroxscaffold_7G00184720
rosa_rugosa Rorug06G0163000
rosa_samantha Rh6AG275500 Rh6BG276100 Rh6CG277900 Rh6DG270700
rosa_wichuraiana Rw6G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 293
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 527
AcsI RAATTY 2 cut(s) 362, 411
AcuI CTGAAG 2 cut(s) 158, 523
AcyI GRCGYC 2 cut(s) 59, 252
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 2 cut(s) 466, 494
AjnI CCWGG 2 cut(s) 93, 194
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AluBI AGCT 5 cut(s) 31, 88, 142, 280, 290
AluI AGCT 5 cut(s) 31, 88, 142, 280, 290
AlwNI CAGNNNCTG 1 cut(s) 88
ApeKI GCWGC 4 cut(s) 31, 36, 47, 68
ApoI RAATTY 2 cut(s) 362, 411
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 91, 124
AsuHPI GGTGA 2 cut(s) 184, 214
AvaII GGWCC 2 cut(s) 91, 124
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 4 cut(s) 18, 23, 34, 55
BccI CCATC 3 cut(s) 49, 269, 350
BceAI ACGGC 2 cut(s) 72, 289
BcgI CGANNNNNNTGC 4 cut(s) 13, 47, 505, 539
BciT130I CCWGG 2 cut(s) 95, 196
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 283
BfuAI ACCTGC 1 cut(s) 581
BisI GCNGC 4 cut(s) 32, 37, 48, 69
BlsI GCNGC 4 cut(s) 33, 38, 49, 70
Bme1390I CCNGG 2 cut(s) 95, 196
Bme18I GGWCC 2 cut(s) 91, 124
BmgT120I GGNCC 2 cut(s) 91, 124
BmiI GGNNCC 4 cut(s) 100, 126, 169, 295
BmrFI CCNGG 2 cut(s) 95, 196
BmsI GCATC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 59, 252
BsaJI CCNNGG 3 cut(s) 94, 186, 237
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 409
BseBI CCWGG 2 cut(s) 95, 196
BseDI CCNNGG 3 cut(s) 94, 186, 237
BseGI GGATG 3 cut(s) 378, 448, 579
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 1 cut(s) 409
BseRI GAGGAG 2 cut(s) 92, 197
BseXI GCAGC 4 cut(s) 18, 23, 34, 55
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 293
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 101
Bsp68I TCGCGA 1 cut(s) 527
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 4 cut(s) 100, 126, 169, 295
BspMI ACCTGC 1 cut(s) 581
BspT107I GGYRCC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 409
BssECI CCNNGG 3 cut(s) 94, 186, 237
BssNI GRCGYC 2 cut(s) 59, 252
Bst2UI CCWGG 2 cut(s) 95, 196
Bst4CI ACNGT 1 cut(s) 513
Bst6I CTCTTC 3 cut(s) 331, 371, 563
BstACI GRCGYC 2 cut(s) 59, 252
BstC8I GCNNGC 3 cut(s) 278, 292, 529
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 1 cut(s) 190
BstF5I GGATG 3 cut(s) 378, 448, 579
BstFNI CGCG 1 cut(s) 527
BstHHI GCGC 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 528, 596
BstNI CCWGG 2 cut(s) 95, 196
BstPI GGTNACC 1 cut(s) 190
BstSCI CCNGG 2 cut(s) 93, 194
BstSFI CTRYAG 1 cut(s) 283
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 4 cut(s) 18, 23, 34, 55
BstV2I GAAGAC 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 2 cut(s) 163, 508
BtsCI GGATG 3 cut(s) 378, 448, 579
BtuMI TCGCGA 1 cut(s) 527
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 3 cut(s) 278, 292, 529
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 91, 124
CseI GACGC 2 cut(s) 48, 260
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
Eam1104I CTCTTC 3 cut(s) 331, 371, 563
EarI CTCTTC 3 cut(s) 331, 371, 563
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 2 cut(s) 91, 124
Eco57I CTGAAG 2 cut(s) 158, 523
Eco91I GGTNACC 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 93, 194
EcoT38I GRGCYC 1 cut(s) 101
FaiI YATR 4 cut(s) 104, 552, 554, 566
FaqI GGGAC 1 cut(s) 187
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 4 cut(s) 32, 37, 48, 69
FokI GGATG 3 cut(s) 385, 455, 586
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 32, 37, 48, 69
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 35
GluI GCNGC 4 cut(s) 32, 37, 48, 69
HgaI GACGC 2 cut(s) 48, 260
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 2 cut(s) 59, 252
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 15, 231
HphI GGTGA 2 cut(s) 184, 214
Hpy166II GTNNAC 2 cut(s) 124, 271
Hpy188I TCNGA 2 cut(s) 7, 172
Hpy188III TCNNGA 2 cut(s) 19, 526
Hpy8I GTNNAC 2 cut(s) 124, 271
Hpy99I CGWCG 4 cut(s) 254, 269, 272, 275
HpyCH4III ACNGT 1 cut(s) 513
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 4 cut(s) 71, 516, 546, 609
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 528, 596
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 2 cut(s) 59, 252
HspAI GCGC 1 cut(s) 34
LmnI GCTCC 4 cut(s) 79, 104, 173, 422
Lsp1109I GCAGC 4 cut(s) 18, 23, 34, 55
LweI GCATC 1 cut(s) 531
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 190, 311
MboII GAAGA 8 cut(s) 208, 211, 257, 348, 365, 388, 580, 593
MfeI CAATTG 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 5 cut(s) 362, 411, 431, 468, 615
MlyI GAGTC 2 cut(s) 24, 225
MmeI TCCRAC 4 cut(s) 195, 330, 388, 417
MspR9I CCNGG 2 cut(s) 95, 196
MunI CAATTG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 2 cut(s) 95, 196
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 528, 596
NlaIV GGNNCC 4 cut(s) 100, 126, 169, 295
NmuCI GTSAC 2 cut(s) 190, 311
NruI TCGCGA 1 cut(s) 527
PaqCI CACCTGC 1 cut(s) 581
PctI GAATGC 1 cut(s) 113
PkrI GCNGC 4 cut(s) 33, 38, 49, 70
PleI GAGTC 2 cut(s) 23, 225
PpsI GAGTC 2 cut(s) 23, 225
Psp6I CCWGG 2 cut(s) 93, 194
PspEI GGTNACC 1 cut(s) 190
PspGI CCWGG 2 cut(s) 93, 194
PspN4I GGNNCC 4 cut(s) 100, 126, 169, 295
PspPI GGNCC 2 cut(s) 91, 124
PstNI CAGNNNCTG 1 cut(s) 88
RruI TCGCGA 1 cut(s) 527
SalI GTCGAC 1 cut(s) 269
SatI GCNGC 4 cut(s) 32, 37, 48, 69
Sau96I GGNCC 2 cut(s) 91, 124
SchI GAGTC 2 cut(s) 24, 225
ScrFI CCNGG 2 cut(s) 95, 196
SduI GDGCHC 1 cut(s) 101
SfaNI GCATC 1 cut(s) 531
SfcI CTRYAG 1 cut(s) 283
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 2 cut(s) 91, 124
Sse9I AATT 5 cut(s) 362, 411, 431, 468, 615
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 93, 194
TaaI ACNGT 1 cut(s) 513
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 270
TaqII GACCGA 1 cut(s) 178
TasI AATT 5 cut(s) 362, 411, 431, 468, 615
TseFI GTSAC 2 cut(s) 190, 311
TseI GCWGC 4 cut(s) 31, 36, 47, 68
Tsp45I GTSAC 2 cut(s) 190, 311
TspDTI ATGAA 3 cut(s) 145, 389, 581
TspGWI ACGGA 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 91, 124
XapI RAATTY 2 cut(s) 362, 411
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.