Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007932

Physical Location & Seq

Genomic Coordinates Forward (+)

31572737 .. 31580679

Gene Structure 7943 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1187600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4608 bp

ATGGGTCCCACTATCCTCCTCCATGGAGGCATCCCTTCGTTTTCTCCAGCAGCTCAGCAATCCTCAATTACCCTTTTGACAGCTCTTCAGGGTTTGCCTGTTTTGGAGCTCGCTTCAATTCTCATCAATTTGGTGCTCTTTGTTGGGTTCCTCTGCATCCTCTTAGCTCGCCGGGTGTTTGGTTGTCTGAGTCGGATTAGAATCCTCAAGGATGATTCGGATTTGAATTCGAGCTCAATTCGGCGTAATAGTGCTGTGGATGGAGGAACTCGTGAGGTTAGAGTTGGAAGAGACTTCAAATTTTCGGTCTTTTGTTGCTTCTATGTGTTGTTTGTGCAAGTTTTGGTGTTGGGTTTTGATGGGGTTGGTCTGATAAGGGAGAGAAGTAATGGGAATGTTGTGGATTGGTCTGTGATAGTCTTGCCTGCTGCACAAGCTTTGGCTTCGTTCGTGCTGAGCTTTTCGGCTCTGCATTGTAAGTTCAAGGCATGTGAGAAATTCCCACTGCTTTTGAGGGTCTGGTGGTCTGTGTCATTTCTGATTTGCTTGTGCACTTTATATGTTGATGGGAGAGCTTTTGCAATTGAAGGATCGAAACACATGAGTTCTCATGTTGTGGCAAATCTCGCTGTGACACCGGCCCTAGCGTTTCTTTGTTTCGTTGCCTGTAGGGGTGTTACTGGTATTCAAGTTTCTGGACCATCTGATCTTCAAGAGCCATTGCTTGAAGAAGAAGCAGGGTGTCTTAAGGTTAGCCCTTATCATGATGCCGGGCTTTTCAGCTTAGCCACACTGTCTTGGATGAACCCGCTTCTTTCAATTGGGGCAAAAAGACCGCTTGAGATCAAGGACATTCCACTTCTTGCACCACAAGATCGAGCCAAAACTAATTATAAGATCTTGAATTCAAATTGGGAGAAAGTGAAGGCTGAAAACCGTTCAGGACAGCCTTCTTTAGCCTGGGCAATTCTCAGGTCCTTTTGGAAGGAGGGAGCTTGTAATGCTGTATTTGCTGGATTAAATACTCTTGTTTCATATGTGGGTCCGTTTATGATTAGCTATTTTGTTGATTATTTGGGGGGGATAGAGACTTTCCCTCATGAAGGGTATATTCTTGCCGGTACTTTCTTTGCAGCAAAGCTTGTTGAGACCTTAACAACCCGGCAGTGGTATCTTGGGGTAGACATTTTGGGTATGCATGTAAGATCAGCTCTAACGGCAATGGTATACCGAAAGGGACTCAGACTTTCCAGCTCGGCCAAGCAGAGTCACACTAGTGGAGAGATTGTTAACTACATGGCTGTTGATGTGCAAAGGATAGGGGACTACTCTTGGTATCTCCAGGACATGTGGATGCTTCCCATGCAAATCATTCTCGCCCTTGCAATTTTGTACAAGAATGTTGGAATCGCTTCTGTTGCAACCTTGATTGCCACCATTATCTCCATTGTTCTTACTGTTCCTGTGGCAAAGATACAAGAAGAATATCAAGACAAGTTGATGACTGCCAAGGATGAAAGAATGAGGAAGACTTCAGAGTGCCTGCGAAACATGAGGATTCTCAAGTTGCAAGCTTGGGAGGACCGGTACCGATTGAAGTTAGAGGAGATGCGTGGTGTGGAGTTCAAGTGGCTACGCAAAGCTCTCTACTCTCAAGCTTTTATTACATTCATGTTCTGGAGCTCTCCTATATTTGTTTCAGCCGTAACTTTTGGTACCTCTATATTCTTGGGTCACCAGCTGACTGCAGGTGGTGTGCTTTCTGCTCTAGCCACTTTCAGGATACTCCAAGAACCACTCAGGAATTTCCCTGACCTTGTATCAATGATGGCACAGACAAAAGTTTCCCTTGATCGAATTTCTGGATTCCTGCAGGAGGAAGAGTTGCAGGAGGACGCAACAATAGTCCTGCCACGAGGCATCTCAACCACGTCAATAGAAATTGAAGATGGTGTCTTTTGCTGGGACCCTTCCTCTCCCAGGCCAACATTATCAGGAATACAGATGAAAGTGGAAAGAGGGATGCGTGTGGCTGTTTGTGGAATGGTTGGGGCTGGCAAGTCAAGCTTTCTCTCTTGCATCCTTGGTGAGATCCCGAAAATCTCCGGTGAAGTAAGGCTATGTGGTACTGCTGCCTATGTTCCTCAGTCAGCATGGATACAGTCTGGAAATATTGAAGAAAATATTCTTTTTGGTAGCCCAATGGATAAACCAAAGTACAAGAAGGTTATCCATGCTTGTTCACTGAAAAAGGATTTGGAACTTTTCTCACATGGAGACCAGACCATAATCGGTGATAGGGGTATAAATCTGAGTGGTGGCCAGAAGCAACGTGTGCAGCTTGCAAGGGCACTGTATCAAGATGCTGACATTTATTTACTTGATGACCCCTTTAGTGCAGTTGATGCACACACTGGGTCAGAGTTGTTTAAGGAATACATATTGACAGCATTAGAAGATAAAACTGTGGTCTTTGTGACCCATCAAGTTGAATTTCTGCCTGCTGCTGATTTGATACTGGTTCTTAAAGGAGGGCGCATCATACAGGCAGGAAAATATGATGATCTTTTACAGGCAGGAACTGATTTCAAATCACTAGTCTCAGCTCACCATGAAGCAATTGAAGCTATGGATATTCCCAATAACTCATCGGGAGATTCAGATCAAAGCTTGTGTCTGGATACTGGGCTCCGAAAAAATTGTGATAAACCTAGCAGTAGTGTTGACTGTTTGGCAAAGGAAGTGCAAGAAGGTGTGTCAGCTTCAGAGCAGAAAGCAATTAAAGAGAAAAAGAAAGCAAAGCGCTCAAGAAAAAAGCAGCTTGTCCAAGAAGAGGAAAGGGTAAGAGGAAGGGTCAGCATGAAGGTCTACTTTTCGTATATGGCTGCAGCATATAAAGGATTGCTGATTCCACCAATAATCATTGCACAAACAGTATTTCAGTTTCTTCAAATTGCTAGTAGTTGGTGGATGGCTTGGGCAAATCCCCAAACAGAAGGAGATCAACCGAAAGTGAGTTCGATGGTCCTTCTTTGTGTTTACATGGCCCTTGCATTCGGAAGCTCTTGGTTTATATTTGTTAGGGCTGTTTTGGTAGCTACGTTTGGTCTAGCAGCTGCTCAGAAATTGTTTGTGAAGATGCTTAGAAGTGTGTTCCGGGCACCAATGTCTTTCTTTGACTCTACTCCTGCTGGACGGATCTTGAATCGTGTATCAATTGATCAAAGTGTGGTGGATCTTGATATTCCTTTTAGACTTGGTGGGTTCGCTTCAACGACAATACAACTTATTGGTATTGTTGGTGTGATGACAACAGTTACCTGGCAAGTTTTGCTGCTTGTTATTCCTATGGCCATTGCTTGTTTGTGGATGCAGAAATACTACATGGCTTCATCAAGGGAACTGGTCCGAATTGTTAGCATCCAGAAGTCTCCAATTATCCATCTTTTTGGCGAGTCAATTGCTGGAGCTGCCACTATAAGAGGATTTGGACAAGAAAAAAGGTTCATGAAGAGGAACCTTTATCTTCTTGATTGTTTCGCTCGCCCTTTCTTCTGCAGTATTGCAGCTATTGAATGGCTCTGTCTGCGCATGGAATTACTCTCAACTTTTGTATTTGCTTTCTGCATGATTCTACTTGTGAGCTTTCCACATGGGAGTATCGATCCAAGCATGGCTGGCCTCGCTGTCACATATGGTCTGAATTTAAATGCACGCCTATCACGATGGATACTTAGCTTTTGCAAGCTTGAAAACAAAATTATTTCTATTGAAAGGATATATCAGTACAGTCAAATCCCAAGTGAAGCTCCATCCGTTATTGAGGATTCTCAACCTCCATCTACATGGCCAGAGAATGGAGCAATTGACATAATTGATTTAAAGGTTCGCTACAAGGAAAATCTTCCTGTTGTCCTTCATGGGGTTACTTGTAGTTTTCCCGGTGGAAAGAATATTGGAATTGTTGGGCGTACTGGAAGTGGTAAATCTACTTTGATCCAGGCATTATTTCGACTAATTGAACCATCAGCTGGGAGGATCCTTATAGACAATGTTGATATTTCGACAATTGGTCTTCATGATCTCCGAAGTCGTCTCAGTATCATACCCCAGGATCCTACCTTATTTGAAGGGACCATTAGGCTCAATCTTGATCCCCTTGAAGAGCATTTGGATCATGAAATTTGGCAGGCACTTGACAAGTCTCAGCTTGGAGACATAATCCGTGAAAAGGAGCAAAAGCTTGATGCACCAGTACTGGAAAATGGAGATAATTGGAGTGTGGGTCAGCGGCAACTGGTTTCTCTTGGAAGAGCATTGCTTAAGCAGGCAAAAATATTGGTGCTTGATGAAGCAACAGCATCGGTTGATACAGCTACAGACAATCTTATCCAGAAAATTATTCGAACAGAGTTTAAGAACTGTACTGTGTGCACGATTGCACATCGTATCCCAACCGTCATTGATAGTGATCTAGTGCTGGTACTCAGTGATGGTCGAGTGGCAGAGTTTGATACTCCTATACGACTATTAGAAGATAAGTCATCCATGTTTCTAAAATTGGTGACAGAGTATTCATCTAGGTCGAGTGGCATACCAGAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004888 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006873 GO:0006875 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008281 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0010118 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022804 GO:0022857 GO:0023052 GO:0030003 GO:0030004 GO:0030007 GO:0031090 GO:0032870 GO:0033993 GO:0038023 GO:0042221 GO:0042592 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0097305 GO:0097306 GO:0098588 GO:0098771 GO:0098805 GO:1901527 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1536

Amino Acids

170.26

Weight (kDa)

8.22

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	335 - 600	1.1e-29	ABC transporter transmembrane region
ABC_tran	PF00005	664 - 798	1.2e-18	ABC transporter
ABC_membrane	PF00664	973 - 1214	9.2e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1306 - 1454	8e-27	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04120 AT1G04120 AT1G04120
fragaria_vesca	FvH4_2g20350 FvH4_2g20350 FvH4_2g20350
malus_domestica	MD05G1187600.v1.1 MD10G1175300.v1.1
prunus_persica	Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1
pyrus_communis	pycom05g17240 pycom10g15100
rosa_chinensis	RchiOBHm_Chr6g0286471
rosa_laevigata	RLG00000012569
rosa_multiflora	Rmu_sc0034370.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182310
rosa_rugosa	Rorug06G0179900 Rorug06G0180000
rosa_samantha	Rh6AG292200 Rh6BG295800 Rh6CG296500 Rh6DG288300
rosa_wichuraiana	Rw6G025200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	894
AarI	CACCTGC	1 cut(s)	1739
Acc16I	TGCGCA	1 cut(s)	3590
Acc36I	ACCTGC	1 cut(s)	1739
Acc65I	GGTACC	2 cut(s)	1587, 1715
AccB1I	GGYRCC	3 cut(s)	1587, 1715, 3160
AccB7I	CCANNNNNTGG	2 cut(s)	3857, 4031
AccI	GTMKAC	3 cut(s)	1182, 1227, 2868
AciI	CCGC	3 cut(s)	809, 836, 4291
AcoI	YGGCCR	4 cut(s)	1257, 2320, 3351, 3848
AcuI	CTGAAG	3 cut(s)	71, 1518, 2748
AfeI	AGCGCT	1 cut(s)	2804
AflII	CTTAAG	2 cut(s)	746, 4322
AflIII	ACRYGT	2 cut(s)	1347, 2332
AgeI	ACCGGT	1 cut(s)	1584
AhdI	GACNNNNNGTC	1 cut(s)	4071
AhlI	ACTAGT	2 cut(s)	1274, 2596
AjiI	CACGTC	1 cut(s)	1932
AjnI	CCWGG	6 cut(s)	959, 1341, 1979, 3320, 3999, 4110
AjuI	GAANNNNNNNTTGG	2 cut(s)	2240, 2272
AleI	CACNNNNGTG	1 cut(s)	1275
Alw21I	GWGCWC	6 cut(s)	111, 138, 236, 554, 1685, 4436
Alw26I	GTCTC	9 cut(s)	285, 1082, 1142, 2271, 2605, 3435, 4100, 4209, 4209
Alw44I	GTGCAC	2 cut(s)	550, 4432
AlwNI	CAGNNNCTG	1 cut(s)	2582
Aor51HI	AGCGCT	1 cut(s)	2804
AoxI	GGCC	8 cut(s)	639, 1257, 1982, 2320, 3045, 3351, 3679, 3848
ApaLI	GTGCAC	2 cut(s)	550, 4432
ArsI	GACNNNNNNTTYG	6 cut(s)	291, 323, 2583, 2615, 2659, 2691
AsiGI	ACCGGT	1 cut(s)	1584
Asp700I	GAANNNNTTC	3 cut(s)	1411, 2184, 3903
Asp718I	GGTACC	2 cut(s)	1587, 1715
AspLEI	GCGC	3 cut(s)	2538, 2805, 3591
AsuC2I	CCSGG	5 cut(s)	173, 772, 1162, 3158, 3942
AsuHPI	GGTGA	6 cut(s)	1727, 2099, 2120, 2306, 2600, 4576
AsuII	TTCGAA	1 cut(s)	4405
AvaII	GGWCC	9 cut(s)	5, 698, 975, 1043, 1582, 1966, 3025, 3406, 4134
BaeGI	GKGCMC	4 cut(s)	554, 2353, 3163, 4436
BalI	TGGCCA	3 cut(s)	2322, 3353, 3850
BamHI	GGATCC	2 cut(s)	4038, 4114
BanI	GGYRCC	3 cut(s)	1587, 1715, 3160
BanII	GRGCYC	4 cut(s)	111, 236, 1685, 2691
BauI	CACGAG	2 cut(s)	270, 1914
BbsI	GAAGAC	2 cut(s)	1535, 4067
Bbv12I	GWGCWC	6 cut(s)	111, 138, 236, 554, 1685, 4436
BceAI	ACGGC	2 cut(s)	1233, 1688
BcgI	CGANNNNNNTGC	4 cut(s)	3443, 3477, 4344, 4378
BciT130I	CCWGG	6 cut(s)	961, 1343, 1981, 3322, 4001, 4112
BciVI	GTATCC	5 cut(s)	1776, 2151, 2674, 3723, 4460
BclI	TGATCA	1 cut(s)	3220
BcnI	CCSGG	5 cut(s)	173, 772, 1162, 3158, 3942
BcoDI	GTCTC	9 cut(s)	285, 1082, 1142, 2271, 2605, 3435, 4100, 4209, 4209
BcuI	ACTAGT	2 cut(s)	1274, 2596
BfaI	CTAG	9 cut(s)	644, 1275, 1769, 2597, 2712, 2958, 3110, 4475, 4581
BfmI	CTRYAG	6 cut(s)	667, 1746, 1871, 2886, 3556, 4377
BfoI	RGCGCY	1 cut(s)	2806
BfrI	CTTAAG	2 cut(s)	746, 4322
BfuAI	ACCTGC	1 cut(s)	1739
BfuI	GTATCC	5 cut(s)	1776, 2151, 2674, 3723, 4460
BglII	AGATCT	1 cut(s)	897
BlpI	GCTNAGC	3 cut(s)	54, 455, 784
BmcAI	AGTACT	1 cut(s)	4257
Bme18I	GGWCC	9 cut(s)	5, 698, 975, 1043, 1582, 1966, 3025, 3406, 4134
BmeRI	GACNNNNNGTC	1 cut(s)	4071
BmgBI	CACGTC	1 cut(s)	1932
BmrI	ACTGGG	2 cut(s)	2424, 2694
BmuI	ACTGGG	2 cut(s)	2424, 2694
BpiI	GAAGAC	2 cut(s)	1535, 4067
BpmI	CTGGAG	4 cut(s)	30, 1325, 1699, 3486
Bpu1102I	GCTNAGC	3 cut(s)	54, 455, 784
Bpu14I	TTCGAA	1 cut(s)	4405
BpuEI	CTTGAG	5 cut(s)	191, 860, 1547, 1638, 2791
BpuMI	CCSGG	5 cut(s)	173, 772, 1162, 3158, 3942
Bsa29I	ATCGAT	1 cut(s)	3663
BsaBI	GATNNNNATC	3 cut(s)	200, 2661, 4470
BsaI	GGTCTC	2 cut(s)	1142, 2271
BsaJI	CCNNGG	6 cut(s)	22, 960, 1509, 1979, 2083, 4110
BsaWI	WCCGGW	2 cut(s)	1584, 2105
Bse118I	RCCGGY	3 cut(s)	637, 1118, 1584
Bse1I	ACTGG	9 cut(s)	685, 2419, 2523, 2689, 3408, 3979, 4253, 4263, 4302
Bse3DI	GCAATG	5 cut(s)	719, 1227, 2922, 3354, 4316
Bse8I	GATNNNNATC	3 cut(s)	200, 2661, 4470
BseBI	CCWGG	6 cut(s)	961, 1343, 1981, 3322, 4001, 4112
BseCI	ATCGAT	1 cut(s)	3663
BseDI	CCNNGG	6 cut(s)	22, 960, 1509, 1979, 2083, 4110
BseJI	GATNNNNATC	3 cut(s)	200, 2661, 4470
BseMI	GCAATG	5 cut(s)	719, 1227, 2922, 3354, 4316
BseNI	ACTGG	9 cut(s)	685, 2419, 2523, 2689, 3408, 3979, 4253, 4263, 4302
BseRI	GAGGAG	2 cut(s)	8, 1619
BseSI	GKGCMC	4 cut(s)	554, 2353, 3163, 4436
BseYI	CCCAGC	2 cut(s)	1962, 4031
BsgI	GTGCAG	3 cut(s)	414, 2357, 2418
BshFI	GGCC	8 cut(s)	641, 1259, 1984, 2322, 3047, 3353, 3681, 3850
BshNI	GGYRCC	3 cut(s)	1587, 1715, 3160
BshTI	ACCGGT	1 cut(s)	1584
BshVI	ATCGAT	1 cut(s)	3663
BsiHKAI	GWGCWC	6 cut(s)	111, 138, 236, 554, 1685, 4436
BsiSI	CCGG	9 cut(s)	172, 638, 771, 1119, 1162, 1585, 2106, 3157, 3942
BslFI	GGGAC	4 cut(s)	1251, 1337, 1979, 4147
BsmAI	GTCTC	9 cut(s)	285, 1082, 1142, 2271, 2605, 3435, 4100, 4209, 4209
BsmBI	CGTCTC	1 cut(s)	4100
BsmFI	GGGAC	4 cut(s)	1251, 1337, 1979, 4147
BsmI	GAATGC	1 cut(s)	3053
BsnI	GGCC	8 cut(s)	641, 1259, 1984, 2322, 3047, 3353, 3681, 3850
Bso31I	GGTCTC	2 cut(s)	1142, 2271
Bsp119I	TTCGAA	1 cut(s)	4405
Bsp1286I	GDGCHC	9 cut(s)	111, 138, 236, 554, 1685, 2353, 2691, 3163, 4436
Bsp1407I	TGTACA	1 cut(s)	1392
Bsp1720I	GCTNAGC	3 cut(s)	54, 455, 784
Bsp19I	CCATGG	1 cut(s)	22
BspACI	CCGC	3 cut(s)	809, 836, 4291
BspANI	GGCC	8 cut(s)	641, 1259, 1984, 2322, 3047, 3353, 3681, 3850
BspDI	ATCGAT	1 cut(s)	3663
BspHI	TCATGA	5 cut(s)	763, 1099, 3507, 4078, 4177
BspMAI	CTGCAG	4 cut(s)	1750, 1875, 2890, 3560
BspMI	ACCTGC	1 cut(s)	1739
BspQI	GCTCTTC	3 cut(s)	90, 4158, 4306
BspT104I	TTCGAA	1 cut(s)	4405
BspT107I	GGYRCC	3 cut(s)	1587, 1715, 3160
BspTI	CTTAAG	2 cut(s)	746, 4322
BspTNI	GGTCTC	2 cut(s)	1142, 2271
BsrDI	GCAATG	5 cut(s)	719, 1227, 2922, 3354, 4316
BsrFI	RCCGGY	3 cut(s)	637, 1118, 1584
BsrGI	TGTACA	1 cut(s)	1392
BsrI	ACTGG	9 cut(s)	685, 2419, 2523, 2689, 3408, 3979, 4253, 4263, 4302
BssAI	RCCGGY	3 cut(s)	637, 1118, 1584
BssECI	CCNNGG	6 cut(s)	22, 960, 1509, 1979, 2083, 4110
BssNAI	GTATAC	1 cut(s)	1228
BssSI	CACGAG	2 cut(s)	270, 1914
BssT1I	CCWWGG	3 cut(s)	22, 1509, 2083
Bst1107I	GTATAC	1 cut(s)	1228
Bst2BI	CACGAG	2 cut(s)	270, 1914
Bst2UI	CCWGG	6 cut(s)	961, 1343, 1981, 3322, 4001, 4112
Bst6I	CTCTTC	7 cut(s)	90, 283, 1875, 2826, 3506, 4158, 4306
BstAFI	CTTAAG	2 cut(s)	746, 4322
BstAPI	GCANNNNNTGC	3 cut(s)	2335, 2405, 3331
BstAUI	TGTACA	1 cut(s)	1392
BstBI	TTCGAA	1 cut(s)	4405
BstDSI	CCRYGG	1 cut(s)	22
BstEII	GGTNACC	1 cut(s)	1733
BstENI	CCTNNNNNAGG	3 cut(s)	1101, 1874, 1978
BstH2I	RGCGCY	1 cut(s)	2806
BstHHI	GCGC	3 cut(s)	2538, 2805, 3591
BstMAI	GTCTC	9 cut(s)	285, 1082, 1142, 2271, 2605, 3435, 4100, 4209, 4209
BstNI	CCWGG	6 cut(s)	961, 1343, 1981, 3322, 4001, 4112
BstNSI	RCATGY	3 cut(s)	492, 1202, 1351
BstPI	GGTNACC	1 cut(s)	1733
BstSFI	CTRYAG	6 cut(s)	667, 1746, 1871, 2886, 3556, 4377
BstSLI	GKGCMC	4 cut(s)	554, 2353, 3163, 4436
BstV2I	GAAGAC	2 cut(s)	1535, 4067
BstX2I	RGATCY	6 cut(s)	897, 2091, 3198, 3235, 4038, 4114
BstXI	CCANNNNNNTGG	2 cut(s)	3449, 3846
BstYI	RGATCY	6 cut(s)	897, 2091, 3198, 3235, 4038, 4114
BstZ17I	GTATAC	1 cut(s)	1228
Bsu15I	ATCGAT	1 cut(s)	3663
BsuI	GTATCC	5 cut(s)	1776, 2151, 2674, 3723, 4460
BsuRI	GGCC	8 cut(s)	641, 1259, 1984, 2322, 3047, 3353, 3681, 3850
BsuTUI	ATCGAT	1 cut(s)	3663
BtgI	CCRYGG	1 cut(s)	22
BtrI	CACGTC	1 cut(s)	1932
BtsI	GCAGTG	2 cut(s)	503, 1172
BtsIMutI	CAGTG	7 cut(s)	503, 791, 1172, 2243, 2351, 2412, 4495
BveI	ACCTGC	1 cut(s)	1739
CaiI	CAGNNNCTG	1 cut(s)	2582
CciI	TCATGA	5 cut(s)	763, 1099, 3507, 4078, 4177
CfoI	GCGC	3 cut(s)	2538, 2805, 3591
Cfr10I	RCCGGY	3 cut(s)	637, 1118, 1584
ClaI	ATCGAT	1 cut(s)	3663
CseI	GACGC	1 cut(s)	1904
CspAI	ACCGGT	1 cut(s)	1584
CspCI	CAANNNNNGTGG	2 cut(s)	492, 527
DraI	TTTAAA	2 cut(s)	3708, 3882
DriI	GACNNNNNGTC	1 cut(s)	4071
EaeI	YGGCCR	4 cut(s)	1257, 2320, 3351, 3848
Eam1104I	CTCTTC	7 cut(s)	90, 283, 1875, 2826, 3506, 4158, 4306
Eam1105I	GACNNNNNGTC	1 cut(s)	4071
EarI	CTCTTC	7 cut(s)	90, 283, 1875, 2826, 3506, 4158, 4306
Ecl136II	GAGCTC	3 cut(s)	109, 234, 1683
Eco130I	CCWWGG	3 cut(s)	22, 1509, 2083
Eco24I	GRGCYC	4 cut(s)	111, 236, 1685, 2691
Eco31I	GGTCTC	2 cut(s)	1142, 2271
Eco47I	GGWCC	9 cut(s)	5, 698, 975, 1043, 1582, 1966, 3025, 3406, 4134
Eco47III	AGCGCT	1 cut(s)	2804
Eco53kI	GAGCTC	3 cut(s)	109, 234, 1683
Eco57I	CTGAAG	3 cut(s)	71, 1518, 2748
Eco91I	GGTNACC	1 cut(s)	1733
EcoICRI	GAGCTC	3 cut(s)	109, 234, 1683
EcoNI	CCTNNNNNAGG	3 cut(s)	1101, 1874, 1978
EcoO109I	RGGNCCY	3 cut(s)	5, 975, 1966
EcoO65I	GGTNACC	1 cut(s)	1733
EcoRI	GAATTC	2 cut(s)	226, 904
EcoRII	CCWGG	6 cut(s)	959, 1341, 1979, 3320, 3999, 4110
EcoT14I	CCWWGG	3 cut(s)	22, 1509, 2083
EcoT22I	ATGCAT	1 cut(s)	1200
EcoT38I	GRGCYC	4 cut(s)	111, 236, 1685, 2691
ErhI	CCWWGG	3 cut(s)	22, 1509, 2083
Esp3I	CGTCTC	1 cut(s)	4100
FalI	AAGNNNNNCTT	6 cut(s)	1833, 1865, 2051, 2083, 2855, 2887
FaqI	GGGAC	4 cut(s)	1251, 1337, 1979, 4147
FauI	CCCGC	1 cut(s)	816
FauNDI	CATATG	2 cut(s)	1036, 3694
FbaI	TGATCA	1 cut(s)	3220
FblI	GTMKAC	3 cut(s)	1182, 1227, 2868
FriOI	GRGCYC	4 cut(s)	111, 236, 1685, 2691
FspBI	CTAG	9 cut(s)	644, 1275, 1769, 2597, 2712, 2958, 3110, 4475, 4581
FspI	TGCGCA	1 cut(s)	3590
GlaI	GCGC	3 cut(s)	2537, 2804, 3590
GsaI	CCCAGC	2 cut(s)	1966, 4035
GsuI	CTGGAG	4 cut(s)	30, 1325, 1699, 3486
HaeII	RGCGCY	1 cut(s)	2806
HaeIII	GGCC	8 cut(s)	641, 1259, 1984, 2322, 3047, 3353, 3681, 3850
HapII	CCGG	9 cut(s)	172, 638, 771, 1119, 1162, 1585, 2106, 3157, 3942
HgaI	GACGC	1 cut(s)	1904
HhaI	GCGC	3 cut(s)	2538, 2805, 3591
Hin6I	GCGC	3 cut(s)	2536, 2803, 3589
HinP1I	GCGC	3 cut(s)	2536, 2803, 3589
HincII	GTYRAC	2 cut(s)	1291, 2725
HindII	GTYRAC	2 cut(s)	1291, 2725
HindIII	AAGCTT	8 cut(s)	435, 1139, 1572, 1656, 2065, 2668, 3746, 4241
HpaI	GTTAAC	1 cut(s)	1291
HpaII	CCGG	9 cut(s)	172, 638, 771, 1119, 1162, 1585, 2106, 3157, 3942
HphI	GGTGA	6 cut(s)	1727, 2099, 2120, 2306, 2600, 4576
Hpy166II	GTNNAC	9 cut(s)	552, 1183, 1228, 1291, 2243, 2725, 2869, 3040, 4434
Hpy8I	GTNNAC	9 cut(s)	552, 1183, 1228, 1291, 2243, 2725, 2869, 3040, 4434
HpyCH4IV	ACGT	3 cut(s)	1931, 2332, 3101
HpySE526I	ACGT	3 cut(s)	1931, 2332, 3101
HspAI	GCGC	3 cut(s)	2536, 2803, 3589
KflI	GGGWCCC	2 cut(s)	5, 1966
KpnI	GGTACC	2 cut(s)	1591, 1719
Ksp22I	TGATCA	1 cut(s)	3220
KspAI	GTTAAC	1 cut(s)	1291
LguI	GCTCTTC	3 cut(s)	90, 4158, 4306
LmnI	GCTCC	8 cut(s)	106, 992, 1680, 2694, 3467, 3814, 3860, 4234
MaeI	CTAG	9 cut(s)	644, 1275, 1769, 2597, 2712, 2958, 3110, 4475, 4581
MaeII	ACGT	3 cut(s)	1931, 2332, 3101
MfeI	CAATTG	7 cut(s)	582, 819, 2619, 3216, 3459, 3864, 4068
MflI	RGATCY	6 cut(s)	897, 2091, 3198, 3235, 4038, 4114
MhlI	GDGCHC	9 cut(s)	111, 138, 236, 554, 1685, 2353, 2691, 3163, 4436
MlsI	TGGCCA	3 cut(s)	2322, 3353, 3850
MluNI	TGGCCA	3 cut(s)	2322, 3353, 3850
MlyI	GAGTC	5 cut(s)	199, 1233, 1276, 3173, 3464
MmeI	TCCRAC	3 cut(s)	173, 265, 1384
Mox20I	TGGCCA	3 cut(s)	2322, 3353, 3850
Mph1103I	ATGCAT	1 cut(s)	1200
MroXI	GAANNNNTTC	3 cut(s)	1411, 2184, 3903
MscI	TGGCCA	3 cut(s)	2322, 3353, 3850
MslI	CAYNNNNRTG	3 cut(s)	1275, 1352, 3844
Msp20I	TGGCCA	3 cut(s)	2322, 3353, 3850
MspA1I	CMGCKG	4 cut(s)	1741, 3116, 4031, 4291
MspCI	CTTAAG	2 cut(s)	746, 4322
MspI	CCGG	9 cut(s)	172, 638, 771, 1119, 1162, 1585, 2106, 3157, 3942
MunI	CAATTG	7 cut(s)	582, 819, 2619, 3216, 3459, 3864, 4068
Mva1269I	GAATGC	1 cut(s)	3053
MvaI	CCWGG	6 cut(s)	961, 1343, 1981, 3322, 4001, 4112
NciI	CCSGG	5 cut(s)	173, 772, 1162, 3158, 3942
NcoI	CCATGG	1 cut(s)	22
NdeI	CATATG	2 cut(s)	1036, 3694
NmeAIII	GCCGAG	1 cut(s)	1235
NmuCI	GTSAC	6 cut(s)	631, 1268, 1733, 2476, 3688, 4564
NsbI	TGCGCA	1 cut(s)	3590
NsiI	ATGCAT	1 cut(s)	1200
NspI	RCATGY	3 cut(s)	492, 1202, 1351
NspV	TTCGAA	1 cut(s)	4405
OliI	CACNNNNGTG	1 cut(s)	1275
PagI	TCATGA	5 cut(s)	763, 1099, 3507, 4078, 4177
PaqCI	CACCTGC	1 cut(s)	1739
PciI	ACATGT	1 cut(s)	1347
PciSI	GCTCTTC	3 cut(s)	90, 4158, 4306
PcsI	WCGNNNNNNNCGW	1 cut(s)	3721
PctI	GAATGC	1 cut(s)	3053
PdmI	GAANNNNTTC	3 cut(s)	1411, 2184, 3903
PflMI	CCANNNNNTGG	2 cut(s)	3857, 4031
PfoI	TCCNGGA	1 cut(s)	1341
PinAI	ACCGGT	1 cut(s)	1584
PleI	GAGTC	5 cut(s)	198, 1233, 1275, 3173, 3463
PpsI	GAGTC	5 cut(s)	198, 1233, 1275, 3173, 3463
PpuMI	RGGWCCY	3 cut(s)	5, 975, 1966
PscI	ACATGT	1 cut(s)	1347
PsiI	TTATAA	1 cut(s)	894
Psp124BI	GAGCTC	3 cut(s)	111, 236, 1685
Psp5II	RGGWCCY	3 cut(s)	5, 975, 1966
Psp6I	CCWGG	6 cut(s)	959, 1341, 1979, 3320, 3999, 4110
PspEI	GGTNACC	1 cut(s)	1733
PspFI	CCCAGC	2 cut(s)	1962, 4031
PspGI	CCWGG	6 cut(s)	959, 1341, 1979, 3320, 3999, 4110
PspPPI	RGGWCCY	3 cut(s)	5, 975, 1966
PstI	CTGCAG	4 cut(s)	1750, 1875, 2890, 3560
PstNI	CAGNNNCTG	1 cut(s)	2582
PsuI	RGATCY	6 cut(s)	897, 2091, 3198, 3235, 4038, 4114
PvuII	CAGCTG	3 cut(s)	1741, 3116, 4031
RseI	CAYNNNNRTG	3 cut(s)	1275, 1352, 3844
SacI	GAGCTC	3 cut(s)	111, 236, 1685
SapI	GCTCTTC	3 cut(s)	90, 4158, 4306
SbfI	CCTGCAGG	1 cut(s)	1875
ScaI	AGTACT	1 cut(s)	4257
SchI	GAGTC	5 cut(s)	199, 1233, 1276, 3173, 3464
SdaI	CCTGCAGG	1 cut(s)	1875
SduI	GDGCHC	9 cut(s)	111, 138, 236, 554, 1685, 2353, 2691, 3163, 4436
SfcI	CTRYAG	6 cut(s)	667, 1746, 1871, 2886, 3556, 4377
SfuI	TTCGAA	1 cut(s)	4405
SinI	GGWCC	9 cut(s)	5, 698, 975, 1043, 1582, 1966, 3025, 3406, 4134
SmiI	ATTTAAAT	1 cut(s)	3708
SmiMI	CAYNNNNRTG	3 cut(s)	1275, 1352, 3844
SmlI	CTYRAG	7 cut(s)	206, 746, 839, 1562, 1653, 2806, 4322
SmoI	CTYRAG	7 cut(s)	206, 746, 839, 1562, 1653, 2806, 4322
SpeI	ACTAGT	2 cut(s)	1274, 2596
Sse8387I	CCTGCAGG	1 cut(s)	1875
SsiI	CCGC	3 cut(s)	809, 836, 4291
SspI	AATATT	4 cut(s)	2173, 2185, 3955, 4338
SspMI	CTAG	9 cut(s)	644, 1275, 1769, 2597, 2712, 2958, 3110, 4475, 4581
SstI	GAGCTC	3 cut(s)	111, 236, 1685
StyI	CCWWGG	3 cut(s)	22, 1509, 2083
SwaI	ATTTAAAT	1 cut(s)	3708
TaiI	ACGT	3 cut(s)	1934, 2335, 3104
TaqII	GACCGA	1 cut(s)	295
TatI	WGTACW	5 cut(s)	1392, 2217, 3786, 4255, 4424
TauI	GCSGC	1 cut(s)	4294
TscAI	CASTG	7 cut(s)	510, 798, 1172, 2250, 2358, 2419, 4495
TseFI	GTSAC	6 cut(s)	631, 1268, 1733, 2476, 3688, 4564
Tsp45I	GTSAC	6 cut(s)	631, 1268, 1733, 2476, 3688, 4564
TspGWI	ACGGA	4 cut(s)	1035, 3211, 3805, 4214
TspRI	CASTG	7 cut(s)	510, 798, 1172, 2250, 2358, 2419, 4495
Van91I	CCANNNNNTGG	2 cut(s)	3857, 4031
Vha464I	CTTAAG	2 cut(s)	746, 4322
VneI	GTGCAC	2 cut(s)	550, 4432
VpaK11BI	GGWCC	9 cut(s)	5, 698, 975, 1043, 1582, 1966, 3025, 3406, 4134
XagI	CCTNNNNNAGG	3 cut(s)	1101, 1874, 1978
XceI	RCATGY	3 cut(s)	492, 1202, 1351
XmiI	GTMKAC	3 cut(s)	1182, 1227, 2868
XmnI	GAANNNNTTC	3 cut(s)	1411, 2184, 3903
XspI	CTAG	9 cut(s)	644, 1275, 1769, 2597, 2712, 2958, 3110, 4475, 4581
ZrmI	AGTACT	1 cut(s)	4257
Zsp2I	ATGCAT	1 cut(s)	1200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.