Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007932

Physical Location & Seq

Genomic Coordinates Forward (+)

26762312 .. 26770446

Gene Structure 8135 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1175300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4620 bp

ATGGGTCCCACTCTCCTCCTCCTTTTCCAGGGAAGCACCTCCTCAGCTCCTTCGTTATCTCCAGCAGCTCAGCAATGGTCGATTACCCTTTTAACAGCTCTGCAGGCTCTGCCTGTTTTGGAGCTCGCTTCAATTCTCATCAATTTGGTACTCTTTGTTGGGTTCCTCTTCGTCCTCTCAGCTCGACAGGTGTTTGCTTGTCTGGGTCGGATTAGAATCTTCAAGGACAATTCGGGTTCGAACTCGAGCTCAATTCGGCATAATAGTGCTGTGGATGGAGGAACTCATGAGATTAGAGTTGGCACAGACTTCAAATTTTCAGTCTTTTGTTGTTTCTATGTGTTGTTTGTGCAAGTTTTGGTGTTGGGTTTTGATGGGGTTGGTCTGATAAGGGAGAGACGTAATGGGAATGTTGTGGATTGGTCTGTGATGGTCTTGCCTGCTGCACAAGCTTTGGCTTGGTTCGCGCTGAGCTTTTCGGCTCTGCATTGTAAATTCAAGGGGTGTGAGAAATTCCCACTACTTTTGAGGGTCTGGTGGTCAGTGTCATTTCTGATTTGCTTGTGCACTTTATATGTTGATGGGAAAGCTTTTGCAATTGAAGGATTGAAACGCATGAGTTCTCATGTTGTGGCAAATCTTGCTGTGACACCGGCTCTGGCGTTTCTTTGTTTCGTCGCGTTTAGGGGTGTTACTGGTATTCAAGTTTCTGGACAATCTGATCTTCAAGAGCCATTGCTGCAAGAAGAAGCAGGGTGTCTTAAGGTTAGCCCTTATCATGATGCTGGGCTTTTCAGTTTAGCCACACTGTCTTGGATGAACCCGCTTCTTTCAATTGGGGCAAAGAGACCGCTTGAGATCAAGGACATTCCCCTTCTTGCGCCACAAGATCGAGCCAAAACCAATTATAAGATCTTGAATTCAAATTGGGAGAAAGGTAAGGCTGAAAACCCTTCAGGGCAGCCTTCTTTAGCCTGGGCAATTCTCAAGTCCTTTTGGAAGGAGGCAGCTTGTAATGCTGTATTTGCTGGATTGAATACTCTTGTTTCATACGTGGGTCCATTTATGATTAGCTATTTTGTTGATTACTTAGGGGGGATAGAGACTTTCCCCCATGAAGGGTATATCCTTGCTGGTACTTTCTTTGCGGCAAAGCTTGTTGAGACCATAACGACCCGGCAGTGGTATCTCGGGGTAGACATTTTGGGTATGCATGTAAGATCAGCTCTAACGGCAATGGTATACCGAAAGGGACTTAGACTTTCCAGCTCAGCCAAGCAAAGTCACACTAGTGGAGAGATTGTTAACTACATGGCTGTTGATGTGCAAAGGATAGGGGACTACTCTTGGTATCTCCATGACATGTGGATGCTCCCTATGCAAATCATTCTTGCCCTTGCAATTTTGTACAAGAATGTTGGAATTGCTTCTGTTGCGACATTGATTGCCACCATTATCTCCATTGTTCTTACTGTTCCAGTGGCCAAGATACAAGAAGAGTATCAAGACAAGTTGATGACTGCCAAGGATGAAAGAATGAGGAAGACTTCTGAGTGTCTGCGAAACATGAGGATTCTCAAGTTGCAAGCTTGGGAGGACAGGTACCGGTTGAAGTTAGAGGAAATGCGCGGTGTAGAGTTCAAGTGGCTACGCAAAGCTCTCTACTCTCAAGCTTTTATTACATTCATGTTCTGGAGCTCTCCTATATTTGTTTCAGCCGTAACATTTGGTACCTCTATATTCTTGGGTCACCATCTAACTGCAGGTGGTGTTCTTTCTGCTCTAGCCACTTTCAGGATACTCCAAGAACCACTCAGGAATTTCCCTGACCTTGTATCAATGATGGCTCAGACAAAAGTTTCCCTTGATCGAATTTCTGGATTCCTGCAGGAGGAAGAGTTGCAGGAGGATGCAACAATTGTCCTACCAGTAGGCATCACAACCACGTCAATAGAAATTGAAGATGGTGTCTTTTGCTGGGACCCTTCCTCACCCAGGCCAACGTTATCAGGAATACAAATGAAAGTGGAAAAAGGGATGCGAGTGGCTGTTTGTGGAATGGTTGGCGCTGGAAAGTCAAGCTTTCTCTCTTGCATCCTTGGTGAGATTCCAAAAATCTCCGGTGAAGTAAGGCTATGTGGCACTGCTGCTTATGTTCCTCAGTCAGCGTGGATACAGTCTGGAAATATTGAAGAAAATATTCTTTTTGGCAGCCCAATGGATAAACCAAAGTACAAGAAGGTTATCCATGCTTGTTCACTGAAAAAGGATTTGGAACTTTTCTCACATGGAGATCAGACCATAATTGGTGATAGGGGTATAAATCTGAGTGGTGGCCAGAAGCAACGTGTGCAGCTCGCAAGGGCGCTGTATCAAGATGCTGACATTTATTTACTTGATGACCCCTTTAGTGCAGTTGATGCACACACTGGGTCAGAGTTGTTTAAGGAATACATATTGACAGCATTAGAAGATAAAACTGTGGTCTTCGTGACCCATCAAGTTGAATTTCTGCCTGCTGCTGATTTAATACTGGTTCTTAAAGGAGGCCACATCATACAGGCAGGAAAATATGATGATCTTTTGCAGGCAGGAACTGATTTTAAATCACTAGTCTCAGCTCACCATGAAGCAATTGAAGCTATGGATATTCCCAATTATTCATCGGGAGATTCAGATCAAAGCTTGTGTGCAGATATTGGGCTCCGAAAAAATTGTGATAAAGCTAGCAGTAGTGTTGACTGTTTGGCAAAGGTGCAAGAAGGTATGTCAGCTTCAGAGCAGAAAGCAATTAAAGAGAAAAAGAAAGCAAAGCACTCAAGAAAAAAGCAGCTTGTCCAAGAAGAGGAAAGGGTACGAGGAAGGGTGAGCATGAAGGTCTACTTTTCATATATGGCTGCAGCATATAAAGGATTGCTGATTCCACTCATAATCATTGCACAAATAGTATTTCAGTTCCTTCAAATCGCTAGTAGTTGGTGGATGGCTTGGGCAAATCCCCAAACAGAAGGAGACCAACCGAAAGTGAGTTCAATGGTCCTCCTTGTTGTTTACATGGCCCTTGCATTCGGAAGCTCTTGGTTTATATTTGTTAGGGCTGTTTTGGTAGCTACATTTGGTCTAGCAGCTGCTCAAAAATTGTTTGTGAAGATGCTTAGAAGTGTGTTCCGGGCACCAATGTCTTTCTTTGACTCTACTCCTGCTGGACGGATCTTGAATCGTGTATCAATTGATCAAAGTGTGGTGGATCTTGACATTCCCTTTAGACTTGGCGGGTTTGCTTCAACGACAATACAACTTATTGGTATTGTTGGTGTGATGACAACAGTTACCTGGCAAATTTTGCTGCTTGTTATTCCTATGGCCATTGCTTGTTTGTGGATGCAGAAATACTACATGTCTTCATCAAGGGAACTGGTCCGTATTGTTAGCATCCAGAAATCTCCAATTATCCATCTTTTTGGTGAGTCAATTGCTGGAGCTGCCACTATAAGAGGATTTGGACAAGAAAAAAGGTTCATGAAGAGGAATCTTTATCTTCTTGATTGCTTTGCTCGCCCATTCTTCTGCAGTATTGCAGCTATTGAATGGCTCTGTCTGCGCATGGAATTACTCTCAACTTTTGTATTCGCTTTCTGCATGATTTTACTTGTGAGTTTTCCACATGGGAGTATCGATCCAAGCATGGCTGGCCTCGCTGTCACATATGGTCTGAATTTAAATGCACGCCTATCACGATGGATACTTAGCTTTTGCAAGCTTGAAAACAAAATTATTTCCATTGAAAGGATATATCAGTACAGTCAAATCCCAAGTGAAGCTCCATCCCTTATTGAGGATTCTCAACCTCCATCTACATGGCCAGAGAATGGAACAATTGACATAATTGATTTAAAGGTTCGCTACAAGGAAAATCTTCCTGTCGTCCTTCATGGGGTAACCTGTAGTTTTCCTGGTGGACAGAAGATTGGAATTGTTGGGCGTACTGGAAGTGGTAAATCTACCTTGATCCAGGCATTATTTCGACTTATTGAACCATCAGCTGGGAGGATCCTTATAGACAATGTTGATATTTCGACGATTGGTCTTCATGATCTCCGAAGCCGTCTCAGTATCATACCCCAGGACCCTACCTTATTTGAAGGGACCATTAGGCTCAATCTTGATCCCCTTGAAGAGCATTTGGATCATGAGATTTGGCAGGCACTTGACAAATCTCAGCTTGGAGATGTAATCCGTGAGAAAGAGCAAAGGCTTGATGCACCAGTGCTGGAAAATGGAGATAATTGGAGTGTGGGGCAGCGGCAACTTGTTTCTCTCGGAAGAGCATTGCTTAAGCAGGCAAAAATATTGGTGCTTGATGAGGCGACAGCATCAGTTGATACAGCCACAGACAATCTCATCCAGAAGATTATTCGTACAGAGTTTAAGAACTGCACCGTGTGCACTATCGCACATCGTATTCCAACCGTCATTGACAGTGATCTAGTACTGGTACTCAGTGATGGTCGAGTTGCAGAGTTCGATACTCCTACACGACTATTAGAAGATAAGTCATCCATGTTTCTAAAATTGGTAACAGAGTATTCCTCTAGGTCAAGTGGCATACCAGAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004888 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006873 GO:0006875 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008281 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0010118 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022804 GO:0022857 GO:0023052 GO:0030003 GO:0030004 GO:0030007 GO:0031090 GO:0032870 GO:0033993 GO:0038023 GO:0042221 GO:0042592 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0097305 GO:0097306 GO:0098588 GO:0098771 GO:0098805 GO:1901527 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1540

Amino Acids

170.72

Weight (kDa)

8.07

Isoelectric Point (pI)

42.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	340 - 605	3e-30	ABC transporter transmembrane region
ABC_tran	PF00005	669 - 803	1.2e-18	ABC transporter
ABC_membrane	PF00664	976 - 1218	1.7e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1310 - 1458	5.1e-27	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04120 AT1G04120 AT1G04120
fragaria_vesca	FvH4_2g20350 FvH4_2g20350 FvH4_2g20350
malus_domestica	MD05G1187600.v1.1 MD10G1175300.v1.1
prunus_persica	Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1
pyrus_communis	pycom05g17240 pycom10g15100
rosa_chinensis	RchiOBHm_Chr6g0286471
rosa_laevigata	RLG00000012569
rosa_multiflora	Rmu_sc0034370.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182310
rosa_rugosa	Rorug06G0179900 Rorug06G0180000
rosa_samantha	Rh6AG292200 Rh6BG295800 Rh6CG296500 Rh6DG288300
rosa_wichuraiana	Rw6G025200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	909
AarI	CACCTGC	1 cut(s)	1754
Acc16I	TGCGCA	1 cut(s)	3602
Acc36I	ACCTGC	1 cut(s)	1754
Acc65I	GGTACC	2 cut(s)	1602, 1730
AccB1I	GGYRCC	3 cut(s)	1602, 1730, 3172
AccB7I	CCANNNNNTGG	2 cut(s)	3869, 4043
AccI	GTMKAC	3 cut(s)	1197, 1242, 2880
AccII	CGCG	3 cut(s)	467, 680, 1629
AciI	CCGC	6 cut(s)	824, 851, 1148, 1629, 3273, 4303
AclI	AACGTT	1 cut(s)	2003
AclWI	GGATC	8 cut(s)	3218, 3255, 3671, 4003, 4045, 4058, 4160, 4194
AcoI	YGGCCR	4 cut(s)	1482, 2335, 3363, 3860
AcsI	RAATTY	9 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3339, 3715
AcuI	CTGAAG	2 cut(s)	939, 2760
AdeI	CACNNNGTG	1 cut(s)	4443
AflII	CTTAAG	2 cut(s)	761, 4334
AflIII	ACRYGT	3 cut(s)	1362, 2347, 3396
AgeI	ACCGGT	1 cut(s)	1605
AhdI	GACNNNNNGTC	1 cut(s)	4083
AhlI	ACTAGT	2 cut(s)	1289, 2611
AjiI	CACGTC	1 cut(s)	1947
AjnI	CCWGG	7 cut(s)	27, 974, 1994, 3332, 3952, 4011, 4122
AjuI	GAANNNNNNNTTGG	2 cut(s)	2255, 2287
AleI	CACNNNNGTG	1 cut(s)	1290
Alw21I	GWGCWC	5 cut(s)	126, 251, 569, 1700, 4448
Alw26I	GTCTC	7 cut(s)	391, 841, 1097, 1157, 2620, 3006, 4112
Alw44I	GTGCAC	2 cut(s)	565, 4444
AlwI	GGATC	8 cut(s)	3218, 3255, 3671, 4003, 4045, 4058, 4160, 4194
AlwNI	CAGNNNCTG	2 cut(s)	109, 2597
Ama87I	CYCGRG	2 cut(s)	244, 1190
AoxI	GGCC	8 cut(s)	1482, 1997, 2335, 2548, 3057, 3363, 3691, 3860
ApaLI	GTGCAC	2 cut(s)	565, 4444
ApoI	RAATTY	9 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3339, 3715
ArsI	GACNNNNNNTTYG	2 cut(s)	306, 338
AsiGI	ACCGGT	1 cut(s)	1605
Asp700I	GAANNNNTTC	3 cut(s)	1426, 2199, 3915
Asp718I	GGTACC	2 cut(s)	1602, 1730
AspLEI	GCGC	6 cut(s)	469, 883, 1629, 2069, 2368, 3603
AspS9I	GGNCC	8 cut(s)	5, 1058, 1981, 3037, 3058, 3418, 4126, 4146
AsuC2I	CCSGG	2 cut(s)	1177, 3170
AsuHPI	GGTGA	8 cut(s)	1742, 1983, 2114, 2135, 2321, 2615, 2878, 3476
AsuII	TTCGAA	1 cut(s)	239
AsuNHI	GCTAGC	1 cut(s)	2726
AvaI	CYCGRG	2 cut(s)	244, 1190
AvaII	GGWCC	7 cut(s)	5, 1058, 1981, 3037, 3418, 4126, 4146
BaeGI	GKGCMC	3 cut(s)	569, 3175, 4448
BalI	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
BamHI	GGATCC	1 cut(s)	4050
BanI	GGYRCC	3 cut(s)	1602, 1730, 3172
BanII	GRGCYC	4 cut(s)	126, 251, 1700, 2706
BarI	GAAGNNNNNNTAC	2 cut(s)	2758, 2790
BbsI	GAAGAC	4 cut(s)	1550, 2479, 3393, 4079
Bbv12I	GWGCWC	5 cut(s)	126, 251, 569, 1700, 4448
BbvCI	CCTCAGC	1 cut(s)	43
BceAI	ACGGC	3 cut(s)	1248, 1703, 4089
BciT130I	CCWGG	7 cut(s)	29, 976, 1996, 3334, 3954, 4013, 4124
BciVI	GTATCC	3 cut(s)	1791, 2166, 3735
BclI	TGATCA	1 cut(s)	3232
BcnI	CCSGG	2 cut(s)	1177, 3170
BcoDI	GTCTC	7 cut(s)	391, 841, 1097, 1157, 2620, 3006, 4112
BcuI	ACTAGT	2 cut(s)	1289, 2611
BfaI	CTAG	8 cut(s)	1290, 1784, 2612, 2727, 2970, 3122, 4487, 4593
BfmI	CTRYAG	6 cut(s)	101, 1761, 1886, 2898, 3568, 3943
BfoI	RGCGCY	2 cut(s)	2070, 2369
BfrI	CTTAAG	2 cut(s)	761, 4334
BfuAI	ACCTGC	1 cut(s)	1754
BfuI	GTATCC	3 cut(s)	1791, 2166, 3735
BglII	AGATCT	1 cut(s)	912
BlpI	GCTNAGC	3 cut(s)	69, 470, 1270
BmcAI	AGTACT	1 cut(s)	4491
Bme1390I	CCNGG	9 cut(s)	29, 976, 1177, 1996, 3170, 3334, 3954, 4013, 4124
Bme18I	GGWCC	7 cut(s)	5, 1058, 1981, 3037, 3418, 4126, 4146
BmeRI	GACNNNNNGTC	1 cut(s)	4083
BmeT110I	CYCGRG	2 cut(s)	244, 1190
BmgBI	CACGTC	1 cut(s)	1947
BmgT120I	GGNCC	8 cut(s)	5, 1058, 1981, 3037, 3058, 3418, 4126, 4146
BmrFI	CCNGG	9 cut(s)	29, 976, 1177, 1996, 3170, 3334, 3954, 4013, 4124
BmrI	ACTGGG	1 cut(s)	2439
BmtI	GCTAGC	1 cut(s)	2730
BmuI	ACTGGG	1 cut(s)	2439
BpiI	GAAGAC	4 cut(s)	1550, 2479, 3393, 4079
BplI	GAGNNNNNCTC	2 cut(s)	4574, 4606
BpmI	CTGGAG	3 cut(s)	45, 1714, 3498
Bpu10I	CCTNAGC	1 cut(s)	43
Bpu1102I	GCTNAGC	3 cut(s)	69, 470, 1270
Bpu14I	TTCGAA	1 cut(s)	239
BpuEI	CTTGAG	5 cut(s)	875, 971, 1562, 1653, 2803
BpuMI	CCSGG	2 cut(s)	1177, 3170
Bsa29I	ATCGAT	1 cut(s)	3675
BsaAI	YACGTR	1 cut(s)	1054
BsaBI	GATNNNNATC	2 cut(s)	215, 2676
BsaI	GGTCTC	3 cut(s)	841, 1157, 3006
BsaJI	CCNNGG	6 cut(s)	28, 975, 1524, 1994, 2098, 4122
BsaWI	WCCGGW	2 cut(s)	1605, 2120
BsaXI	ACNNNNNCTCC	6 cut(s)	1287, 1317, 1586, 1616, 1883, 1913
Bse118I	RCCGGY	2 cut(s)	652, 1605
Bse1I	ACTGG	9 cut(s)	700, 1478, 1928, 2434, 2538, 3420, 3991, 4265, 4497
Bse3DI	GCAATG	6 cut(s)	80, 734, 1242, 2934, 3366, 4328
Bse8I	GATNNNNATC	2 cut(s)	215, 2676
BseBI	CCWGG	7 cut(s)	29, 976, 1996, 3334, 3954, 4013, 4124
BseCI	ATCGAT	1 cut(s)	3675
BseDI	CCNNGG	6 cut(s)	28, 975, 1524, 1994, 2098, 4122
BseJI	GATNNNNATC	2 cut(s)	215, 2676
BseMI	GCAATG	6 cut(s)	80, 734, 1242, 2934, 3366, 4328
BseNI	ACTGG	9 cut(s)	700, 1478, 1928, 2434, 2538, 3420, 3991, 4265, 4497
BseRI	GAGGAG	3 cut(s)	5, 8, 31
BseSI	GKGCMC	3 cut(s)	569, 3175, 4448
BseYI	CCCAGC	3 cut(s)	785, 1977, 4043
BsgI	GTGCAG	5 cut(s)	429, 2372, 2433, 2712, 4420
Bsh1236I	CGCG	3 cut(s)	467, 680, 1629
BshFI	GGCC	8 cut(s)	1484, 1999, 2337, 2550, 3059, 3365, 3693, 3862
BshNI	GGYRCC	3 cut(s)	1602, 1730, 3172
BshTI	ACCGGT	1 cut(s)	1605
BshVI	ATCGAT	1 cut(s)	3675
BsiHKAI	GWGCWC	5 cut(s)	126, 251, 569, 1700, 4448
BsiHKCI	CYCGRG	2 cut(s)	244, 1190
BsiSI	CCGG	5 cut(s)	653, 1177, 1606, 2121, 3169
BslFI	GGGAC	4 cut(s)	1266, 1352, 1994, 4159
BsmAI	GTCTC	7 cut(s)	391, 841, 1097, 1157, 2620, 3006, 4112
BsmBI	CGTCTC	2 cut(s)	391, 4112
BsmFI	GGGAC	4 cut(s)	1266, 1352, 1994, 4159
BsmI	GAATGC	1 cut(s)	3065
BsnI	GGCC	8 cut(s)	1484, 1999, 2337, 2550, 3059, 3365, 3693, 3862
Bso31I	GGTCTC	3 cut(s)	841, 1157, 3006
BsoBI	CYCGRG	2 cut(s)	244, 1190
Bsp119I	TTCGAA	1 cut(s)	239
Bsp1286I	GDGCHC	7 cut(s)	126, 251, 569, 1700, 2706, 3175, 4448
Bsp1407I	TGTACA	1 cut(s)	1407
Bsp1720I	GCTNAGC	3 cut(s)	69, 470, 1270
BspACI	CCGC	6 cut(s)	824, 851, 1148, 1629, 3273, 4303
BspANI	GGCC	8 cut(s)	1484, 1999, 2337, 2550, 3059, 3365, 3693, 3862
BspDI	ATCGAT	1 cut(s)	3675
BspFNI	CGCG	3 cut(s)	467, 680, 1629
BspHI	TCATGA	5 cut(s)	286, 778, 3519, 4090, 4189
BspMAI	CTGCAG	5 cut(s)	105, 1765, 1890, 2902, 3572
BspMI	ACCTGC	1 cut(s)	1754
BspOI	GCTAGC	1 cut(s)	2730
BspPI	GGATC	8 cut(s)	3218, 3255, 3671, 4003, 4045, 4058, 4160, 4194
BspQI	GCTCTTC	2 cut(s)	4170, 4318
BspT104I	TTCGAA	1 cut(s)	239
BspT107I	GGYRCC	3 cut(s)	1602, 1730, 3172
BspTI	CTTAAG	2 cut(s)	761, 4334
BspTNI	GGTCTC	3 cut(s)	841, 1157, 3006
BsrDI	GCAATG	6 cut(s)	80, 734, 1242, 2934, 3366, 4328
BsrFI	RCCGGY	2 cut(s)	652, 1605
BsrGI	TGTACA	1 cut(s)	1407
BsrI	ACTGG	9 cut(s)	700, 1478, 1928, 2434, 2538, 3420, 3991, 4265, 4497
BssAI	RCCGGY	2 cut(s)	652, 1605
BssECI	CCNNGG	6 cut(s)	28, 975, 1524, 1994, 2098, 4122
BssNAI	GTATAC	1 cut(s)	1243
BssT1I	CCWWGG	2 cut(s)	1524, 2098
Bst1107I	GTATAC	1 cut(s)	1243
Bst2UI	CCWGG	7 cut(s)	29, 976, 1996, 3334, 3954, 4013, 4124
Bst6I	CTCTTC	7 cut(s)	173, 1491, 1890, 2838, 3518, 4170, 4318
BstAFI	CTTAAG	2 cut(s)	761, 4334
BstAPI	GCANNNNNTGC	6 cut(s)	109, 641, 2350, 2420, 3343, 4443
BstAUI	TGTACA	1 cut(s)	1407
BstBAI	YACGTR	1 cut(s)	1054
BstBI	TTCGAA	1 cut(s)	239
BstEII	GGTNACC	2 cut(s)	1748, 3937
BstENI	CCTNNNNNAGG	4 cut(s)	26, 1889, 1993, 3833
BstFNI	CGCG	3 cut(s)	467, 680, 1629
BstH2I	RGCGCY	2 cut(s)	2070, 2369
BstHHI	GCGC	6 cut(s)	469, 883, 1629, 2069, 2368, 3603
BstMAI	GTCTC	7 cut(s)	391, 841, 1097, 1157, 2620, 3006, 4112
BstNI	CCWGG	7 cut(s)	29, 976, 1996, 3334, 3954, 4013, 4124
BstNSI	RCATGY	3 cut(s)	1217, 1366, 3400
BstPI	GGTNACC	2 cut(s)	1748, 3937
BstSCI	CCNGG	9 cut(s)	27, 974, 1175, 1994, 3168, 3332, 3952, 4011, 4122
BstSFI	CTRYAG	6 cut(s)	101, 1761, 1886, 2898, 3568, 3943
BstSLI	GKGCMC	3 cut(s)	569, 3175, 4448
BstUI	CGCG	3 cut(s)	467, 680, 1629
BstV2I	GAAGAC	4 cut(s)	1550, 2479, 3393, 4079
BstX2I	RGATCY	4 cut(s)	912, 3210, 3247, 4050
BstXI	CCANNNNNNTGG	2 cut(s)	3461, 3858
BstYI	RGATCY	4 cut(s)	912, 3210, 3247, 4050
BstZ17I	GTATAC	1 cut(s)	1243
Bsu15I	ATCGAT	1 cut(s)	3675
BsuI	GTATCC	3 cut(s)	1791, 2166, 3735
BsuRI	GGCC	8 cut(s)	1484, 1999, 2337, 2550, 3059, 3365, 3693, 3862
BsuTUI	ATCGAT	1 cut(s)	3675
BtrI	CACGTC	1 cut(s)	1947
BtsI	GCAGTG	2 cut(s)	1187, 2142
BveI	ACCTGC	1 cut(s)	1754
CaiI	CAGNNNCTG	2 cut(s)	109, 2597
CciI	TCATGA	5 cut(s)	286, 778, 3519, 4090, 4189
CfoI	GCGC	6 cut(s)	469, 883, 1629, 2069, 2368, 3603
Cfr10I	RCCGGY	2 cut(s)	652, 1605
Cfr13I	GGNCC	8 cut(s)	5, 1058, 1981, 3037, 3058, 3418, 4126, 4146
ClaI	ATCGAT	1 cut(s)	3675
CspAI	ACCGGT	1 cut(s)	1605
CspCI	CAANNNNNGTGG	2 cut(s)	507, 542
DraI	TTTAAA	3 cut(s)	2605, 3720, 3894
DraIII	CACNNNGTG	1 cut(s)	4443
DriI	GACNNNNNGTC	1 cut(s)	4083
EaeI	YGGCCR	4 cut(s)	1482, 2335, 3363, 3860
Eam1104I	CTCTTC	7 cut(s)	173, 1491, 1890, 2838, 3518, 4170, 4318
Eam1105I	GACNNNNNGTC	1 cut(s)	4083
EarI	CTCTTC	7 cut(s)	173, 1491, 1890, 2838, 3518, 4170, 4318
Ecl136II	GAGCTC	3 cut(s)	124, 249, 1698
Eco130I	CCWWGG	2 cut(s)	1524, 2098
Eco24I	GRGCYC	4 cut(s)	126, 251, 1700, 2706
Eco31I	GGTCTC	3 cut(s)	841, 1157, 3006
Eco47I	GGWCC	7 cut(s)	5, 1058, 1981, 3037, 3418, 4126, 4146
Eco53kI	GAGCTC	3 cut(s)	124, 249, 1698
Eco57I	CTGAAG	2 cut(s)	939, 2760
Eco88I	CYCGRG	2 cut(s)	244, 1190
Eco91I	GGTNACC	2 cut(s)	1748, 3937
EcoICRI	GAGCTC	3 cut(s)	124, 249, 1698
EcoNI	CCTNNNNNAGG	4 cut(s)	26, 1889, 1993, 3833
EcoO109I	RGGNCCY	3 cut(s)	5, 1981, 4126
EcoO65I	GGTNACC	2 cut(s)	1748, 3937
EcoRI	GAATTC	1 cut(s)	919
EcoRII	CCWGG	7 cut(s)	27, 974, 1994, 3332, 3952, 4011, 4122
EcoT14I	CCWWGG	2 cut(s)	1524, 2098
EcoT22I	ATGCAT	1 cut(s)	1215
EcoT38I	GRGCYC	4 cut(s)	126, 251, 1700, 2706
ErhI	CCWWGG	2 cut(s)	1524, 2098
Esp3I	CGTCTC	2 cut(s)	391, 4112
FalI	AAGNNNNNCTT	6 cut(s)	1848, 1880, 2066, 2098, 2867, 2899
FaqI	GGGAC	4 cut(s)	1266, 1352, 1994, 4159
FauI	CCCGC	2 cut(s)	831, 3266
FauNDI	CATATG	1 cut(s)	3706
FbaI	TGATCA	1 cut(s)	3232
FblI	GTMKAC	3 cut(s)	1197, 1242, 2880
FriOI	GRGCYC	4 cut(s)	126, 251, 1700, 2706
FspBI	CTAG	8 cut(s)	1290, 1784, 2612, 2727, 2970, 3122, 4487, 4593
FspI	TGCGCA	1 cut(s)	3602
GlaI	GCGC	6 cut(s)	468, 882, 1628, 2068, 2367, 3602
GsaI	CCCAGC	3 cut(s)	789, 1981, 4047
GsuI	CTGGAG	3 cut(s)	45, 1714, 3498
HaeII	RGCGCY	2 cut(s)	2070, 2369
HaeIII	GGCC	8 cut(s)	1484, 1999, 2337, 2550, 3059, 3365, 3693, 3862
HapII	CCGG	5 cut(s)	653, 1177, 1606, 2121, 3169
HhaI	GCGC	6 cut(s)	469, 883, 1629, 2069, 2368, 3603
Hin6I	GCGC	6 cut(s)	467, 881, 1627, 2067, 2366, 3601
HinP1I	GCGC	6 cut(s)	467, 881, 1627, 2067, 2366, 3601
HincII	GTYRAC	2 cut(s)	1306, 2740
HindII	GTYRAC	2 cut(s)	1306, 2740
HindIII	AAGCTT	8 cut(s)	450, 588, 1154, 1587, 1671, 2080, 2683, 3758
HpaI	GTTAAC	1 cut(s)	1306
HpaII	CCGG	5 cut(s)	653, 1177, 1606, 2121, 3169
HphI	GGTGA	8 cut(s)	1742, 1983, 2114, 2135, 2321, 2615, 2878, 3476
Hpy99I	CGWCG	2 cut(s)	680, 4081
HpyCH4IV	ACGT	5 cut(s)	400, 1053, 1946, 2003, 2347
HpySE526I	ACGT	5 cut(s)	400, 1053, 1946, 2003, 2347
HspAI	GCGC	6 cut(s)	467, 881, 1627, 2067, 2366, 3601
KflI	GGGWCCC	2 cut(s)	5, 1981
KpnI	GGTACC	2 cut(s)	1606, 1734
Ksp22I	TGATCA	1 cut(s)	3232
KspAI	GTTAAC	1 cut(s)	1306
LguI	GCTCTTC	2 cut(s)	4170, 4318
LmnI	GCTCC	7 cut(s)	52, 121, 1377, 1695, 2709, 3479, 3826
MaeI	CTAG	8 cut(s)	1290, 1784, 2612, 2727, 2970, 3122, 4487, 4593
MaeII	ACGT	5 cut(s)	400, 1053, 1946, 2003, 2347
MfeI	CAATTG	7 cut(s)	597, 834, 1917, 2634, 3228, 3471, 3876
MflI	RGATCY	4 cut(s)	912, 3210, 3247, 4050
MhlI	GDGCHC	7 cut(s)	126, 251, 569, 1700, 2706, 3175, 4448
MlsI	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
MluNI	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
MlyI	GAGTC	2 cut(s)	3185, 3476
MmeI	TCCRAC	3 cut(s)	188, 1399, 4490
Mox20I	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
Mph1103I	ATGCAT	1 cut(s)	1215
MroXI	GAANNNNTTC	3 cut(s)	1426, 2199, 3915
MscI	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
MslI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3856
Msp20I	TGGCCA	4 cut(s)	1484, 2337, 3365, 3862
MspA1I	CMGCKG	3 cut(s)	3128, 4043, 4303
MspCI	CTTAAG	2 cut(s)	761, 4334
MspI	CCGG	5 cut(s)	653, 1177, 1606, 2121, 3169
MspR9I	CCNGG	9 cut(s)	29, 976, 1177, 1996, 3170, 3334, 3954, 4013, 4124
MunI	CAATTG	7 cut(s)	597, 834, 1917, 2634, 3228, 3471, 3876
Mva1269I	GAATGC	1 cut(s)	3065
MvaI	CCWGG	7 cut(s)	29, 976, 1996, 3334, 3954, 4013, 4124
MvnI	CGCG	3 cut(s)	467, 680, 1629
NciI	CCSGG	2 cut(s)	1177, 3170
NdeI	CATATG	1 cut(s)	3706
NheI	GCTAGC	1 cut(s)	2726
NmuCI	GTSAC	5 cut(s)	646, 1283, 1748, 2491, 3700
NsbI	TGCGCA	1 cut(s)	3602
NsiI	ATGCAT	1 cut(s)	1215
NspI	RCATGY	3 cut(s)	1217, 1366, 3400
NspV	TTCGAA	1 cut(s)	239
OliI	CACNNNNGTG	1 cut(s)	1290
PaeR7I	CTCGAG	1 cut(s)	244
PagI	TCATGA	5 cut(s)	286, 778, 3519, 4090, 4189
PaqCI	CACCTGC	1 cut(s)	1754
PciI	ACATGT	2 cut(s)	1362, 3396
PciSI	GCTCTTC	2 cut(s)	4170, 4318
PcsI	WCGNNNNNNNCGW	1 cut(s)	3733
PctI	GAATGC	1 cut(s)	3065
PdmI	GAANNNNTTC	3 cut(s)	1426, 2199, 3915
PfeI	GAWTC	9 cut(s)	216, 1573, 1881, 2107, 2672, 2920, 3217, 3529, 3839
PflMI	CCANNNNNTGG	2 cut(s)	3869, 4043
PinAI	ACCGGT	1 cut(s)	1605
PleI	GAGTC	2 cut(s)	3185, 3475
PpsI	GAGTC	2 cut(s)	3185, 3475
Ppu21I	YACGTR	1 cut(s)	1054
PpuMI	RGGWCCY	3 cut(s)	5, 1981, 4126
PscI	ACATGT	2 cut(s)	1362, 3396
PsiI	TTATAA	1 cut(s)	909
Psp124BI	GAGCTC	3 cut(s)	126, 251, 1700
Psp1406I	AACGTT	1 cut(s)	2003
Psp5II	RGGWCCY	3 cut(s)	5, 1981, 4126
Psp6I	CCWGG	7 cut(s)	27, 974, 1994, 3332, 3952, 4011, 4122
PspEI	GGTNACC	2 cut(s)	1748, 3937
PspFI	CCCAGC	3 cut(s)	785, 1977, 4043
PspGI	CCWGG	7 cut(s)	27, 974, 1994, 3332, 3952, 4011, 4122
PspPI	GGNCC	8 cut(s)	5, 1058, 1981, 3037, 3058, 3418, 4126, 4146
PspPPI	RGGWCCY	3 cut(s)	5, 1981, 4126
PspXI	VCTCGAGB	1 cut(s)	244
PstI	CTGCAG	5 cut(s)	105, 1765, 1890, 2902, 3572
PstNI	CAGNNNCTG	2 cut(s)	109, 2597
PsuI	RGATCY	4 cut(s)	912, 3210, 3247, 4050
PvuII	CAGCTG	2 cut(s)	3128, 4043
RseI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3856
SacI	GAGCTC	3 cut(s)	126, 251, 1700
SapI	GCTCTTC	2 cut(s)	4170, 4318
Sau96I	GGNCC	8 cut(s)	5, 1058, 1981, 3037, 3058, 3418, 4126, 4146
SbfI	CCTGCAGG	1 cut(s)	1890
ScaI	AGTACT	1 cut(s)	4491
SchI	GAGTC	2 cut(s)	3185, 3476
ScrFI	CCNGG	9 cut(s)	29, 976, 1177, 1996, 3170, 3334, 3954, 4013, 4124
SdaI	CCTGCAGG	1 cut(s)	1890
SduI	GDGCHC	7 cut(s)	126, 251, 569, 1700, 2706, 3175, 4448
SfcI	CTRYAG	6 cut(s)	101, 1761, 1886, 2898, 3568, 3943
Sfr274I	CTCGAG	1 cut(s)	244
SfuI	TTCGAA	1 cut(s)	239
SinI	GGWCC	7 cut(s)	5, 1058, 1981, 3037, 3418, 4126, 4146
SlaI	CTCGAG	1 cut(s)	244
SmiI	ATTTAAAT	1 cut(s)	3720
SmiMI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3856
SmlI	CTYRAG	8 cut(s)	244, 761, 854, 986, 1577, 1668, 2818, 4334
SmoI	CTYRAG	8 cut(s)	244, 761, 854, 986, 1577, 1668, 2818, 4334
SpeI	ACTAGT	2 cut(s)	1289, 2611
Sse8387I	CCTGCAGG	1 cut(s)	1890
SsiI	CCGC	6 cut(s)	824, 851, 1148, 1629, 3273, 4303
SspI	AATATT	3 cut(s)	2188, 2200, 4350
SspMI	CTAG	8 cut(s)	1290, 1784, 2612, 2727, 2970, 3122, 4487, 4593
SstI	GAGCTC	3 cut(s)	126, 251, 1700
StyD4I	CCNGG	9 cut(s)	27, 974, 1175, 1994, 3168, 3332, 3952, 4011, 4122
StyI	CCWWGG	2 cut(s)	1524, 2098
SwaI	ATTTAAAT	1 cut(s)	3720
TaiI	ACGT	5 cut(s)	403, 1056, 1949, 2006, 2350
TatI	WGTACW	4 cut(s)	1407, 2232, 3798, 4489
TauI	GCSGC	2 cut(s)	1151, 4306
TfiI	GAWTC	9 cut(s)	216, 1573, 1881, 2107, 2672, 2920, 3217, 3529, 3839
TseFI	GTSAC	5 cut(s)	646, 1283, 1748, 2491, 3700
Tsp45I	GTSAC	5 cut(s)	646, 1283, 1748, 2491, 3700
TspGWI	ACGGA	3 cut(s)	3223, 3410, 4226
Van91I	CCANNNNNTGG	2 cut(s)	3869, 4043
Vha464I	CTTAAG	2 cut(s)	761, 4334
VneI	GTGCAC	2 cut(s)	565, 4444
VpaK11BI	GGWCC	7 cut(s)	5, 1058, 1981, 3037, 3418, 4126, 4146
XagI	CCTNNNNNAGG	4 cut(s)	26, 1889, 1993, 3833
XapI	RAATTY	9 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3339, 3715
XceI	RCATGY	3 cut(s)	1217, 1366, 3400
XhoI	CTCGAG	1 cut(s)	244
XmiI	GTMKAC	3 cut(s)	1197, 1242, 2880
XmnI	GAANNNNTTC	3 cut(s)	1426, 2199, 3915
XspI	CTAG	8 cut(s)	1290, 1784, 2612, 2727, 2970, 3122, 4487, 4593
ZrmI	AGTACT	1 cut(s)	4491
Zsp2I	ATGCAT	1 cut(s)	1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.