Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0007932

Physical Location & Seq

Genomic Coordinates Forward (+)

48476864 .. 48483622

Gene Structure 6759 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G025200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4560 bp

ATGGGTCTCACTCTCTTGCTTCACAAAATCTCAGCTTCTTCTTCATCTTCAAACCCAGCTCAGCTATCCCAGACGACCCCTTTGGGAGCTGTGCAAGGTCTGCCCCTCCTGGAGCTGTCTTCTATTGTCATCAATTTGGCGCTAGTTCTTGCGTTTCTCTTCATCGTTTCGGCCCGGCGGATGGTTGTCTGTCTGGGCCGGATTAGGGTGCTGAAAGACGATTTGGGTTCGAATGGGAACCCAATTCAGCATGATAGTGCTGTTGATAGGGGAATCCAGGAGGTTAGAGTGGGCACAGACTTTAAATTTTCAGTGTTTTGCTGCTTCTATGTGTTGTTTGTGCAAGTTGTGGTGTTGGGTTTTGATGGGGTTGGTTTGGTTAGAGGAGGAGGGGTTGGTAGTGGGAGGGTGGTGGTTTGGTCTGTGCTTTGCTTACCAGCTGCACAGGGCTTAGCTTGGTTTGTGATGAGCTTCTCGGCTTTGCATTGTAAGTTCAAGGCGGCCGAGAAGTTCCCATTGCTGTTGAGGTGTTGGTGGGCTGTCTCCTTTGTGCTTTGCTTGTGCACTTTGTATGTTGATGGGAGAGGTTTTGTACAAGAAGGCTCGATACACCTTCATTCTCATGTTGTGGCTAATTTCGCAGTCACACCGGCCCTAGCGTTTCTCTGTTTCGTTGGAGTTAGGGGTGTGACTGGTGTGATTGTTTGTAGAAACTCTGAGCTTCAAGAGCCATTGCTTGAAGAAGAAGCAGGGTGTCTTAAGGTTACTCCTTATAGTGATGCTGGGATTTTCAGCTTGGCAACACTATCTTGGATAAACCCTCTTCTTTCAATAGGGGCAAAGAGACCGCTTGAGATCAAGGATATTCCTCTTCTTGCACCACAAGATCGATCCAAGACCAATTATAAGGTCTTGAATTCAAATTGGGAGAAATTGAAGGCTGAAGATCCTTCAAAGCAGCCTTCTTTAGCCTGGGCAATTCTCAAGTCATTTTGGAAGGAGGCAGCTTGTAATGCCATTTTCGCCGGTTTGAACACTCTTGTTTCATACGTGGGTCCGTATATGATCAGCTATTTCGTTGATTACTTGGGGGGAATAGAGACTTTCCCCCATGAAGGGTATATCCTTGCTGGGACCTTCTTTGCAGCGAAGCTAGTTGAGACCTTAACAACCCGGCAGTGGTATCTTGGGGTGGACATTTTGGGTATGCATGTAAGATCAGCTCTAACGGCAATGGTATACCGAAAGGGACTCAGGCTTTCCAGCTCAGCCAAGCAAAGTCACACAAGTGGAGAGATTGTTAACTACATGGCTGTTGATGTGCAGAGGGTAGGGGACTATTCCTGGTATCTCCATGACATATGGATGCTTCCTCTGCAAATTGTTCTTGCCCTTGCAATTTTATACAAGAATGTTGGAATTGCTTCTGTTGCTACATTGATTGCCACCATTATCTCCATTGTTCTTACTGTTCCTTTGGCAAAGATACAAGAAGATTATCAAGACAAGTTGATGACGGCCAAGGATGAAAGAATGAGGAAGACTTCTGAGTGCCTGCGGAACATGAGAATTCTTAAGTTGCAAGCTTGGGAGGACAGATACCGACTGATGTTAGAGGAGATGCGTAGTGTGGAGTTTAAGTATCTACGCAAAGCCCTCTACTCGCAAGCTTTTATCACATTCATGTTCTGGAGCTCTCCTATATTTGTTTCAGCTGTCACTTTCGGTACTTCTGTATTTTTGGGTACTCGTCTCACTGCAGGTGGTGTTCTTTCTGCTCTCGCCACTTTCAGGATCCTCCAAGAACCACTCAGGAATTTCCCTGACCTGGTATCAATGATGGCTCAGACAAAAGTTTCCCTTGATCGTATTTCTGGATTCTTGCAGGAGGAAGAGTTACAGCAGATCACGTCAATAGAAATTAAAGATGGCGTGTTTAGCTGGGACCCTTCCTCTGTAAGACCAACGTTATCGGGAATACAAATGAAAGTGGAAAGAGGGATGCGTGTTGCTGTATGTGGAATGGTTGGTTCTGGAAAATCAAGTTTTCTCTCTTGCATCCTTGGGGAGATCCCGAAAGTTTCCGGTGAAGTAAAGTTATGTGGTACTGCTGCTTATGTTTCTCAGTCAGCATGGATACAGTCTGGTAATATAGAAGAAAATATTCTTTTTGGCAGCCCAATGGAGAAACCAAAGTACAAGAAGGTTATCCATGCTTGTTCACTGAAAAAGGATTTGGAACTTTTCTCACATGGAGATCAAACCATAATTGGTGATAGGGGTATAAATTTGAGTGGTGGCCAGAAGCAACGTGTGCAGCTTGCAAGGGCACTCTATCAGGATGCTGACATTTATTTACTTGACGACCCCTTCAGTGCAGTTGATGCACACACTGGATCAGAGTTGTTTAAGGAATACATATTGACAGCATTAGAAGACAAAACTGTTGTTTTCGTGACCCATCAAGTCGAATTTCTGCCTTCTGCTGATTTAATCCTGGTTCTTAAAGAAGGGCGCATCATACAAGCAGGAAAATATGATGATCTTTTACAGGCAGGAACTGATTTTAAGACACTAGTCTCAGCTCACCATGATGCAATTGAAGCTATGGATATTCCCAATTATTCGTCAGGCGACTCTGATCACAACTTGTGTCCAGATGGCTCTGTTGGACTTGGCAAAAATCCTGATTTACCAAGCAGCAGTGTTGATTGTTTGGCAAAGGAAGTGCAAGAAGGTCCATCAGCTTCAGAGCAGAAAGCAATTAAAGAGAAAAAGAAGGCAAAGCGGGCAAGAAAGAAGCAGCTTGTACAAGAAGAGGAAAGGGTAAGAGGAAGAGTCAGCATGAAGGTTTACTTGTCATATATGGCTGCAGCATATAAAGGATCGCTGATTCCACTCATAATTATTGCACAAGCAATATTTCAATTCCTTCAAATTGCTAGTAGTTGGTGGATGGCTTGGGCGAATCCCCAAACACAGGGAGATCAGCCTAAAGTGAGTGCTATGGTCCTTCTTGGTGTTTACATGGCCCTTGCATTTGGGAGCTCTTGGTTTATATTTATTAGGGCTGTGTTGGTAGCTACCTTTGGTCTAGAAGCTGCACAGAAATTGTTTCTGAAGATGCTTAGGAGTGTTTTCCGTGCACCAATGTCTTTCTTTGATTCTACTCCTGCTGGACGGATCTTGAATCGTGTATCAATTGATCAAAGTGTGGTGGATCTTGATATTCCTTTTAGACTTGGCGGGTTCGCTTCAACGACAATACAGCTTATAGGCATTGTTGGTGTGATGACAAAAGTTACCTGGCAAGTTTTGCTTCTTGTTGTTCCTATGGCAATTGCTTGTTTGTGGATGCAGAAATACTACATGGCTTCATCAAGGGAATTAGTCCGAATTGTTAGCATTCAGAAATCTCCAATTATCCATCTTTTCGGTGAGTCAATTGCAGGGGCGGCCACAATAAGAGGATTTGGACAGGAAAAAAGGTTCATGAAAAGGAATCTTTATTTCCTTGATTGTTTTGCTCGCCCATTCTTCTGCAGTATTGCTGCTATTGAATGGCTCTGCCTACGGATGGAATTACTCTCGACTTTTGTATTTGCTTTCTGCATGCTTTTACTTGTGAGCTTTCCACATGGAACCATTGATCCAAGCATGGCGGGTCTTGCTGTAACATATGGCTTGAATTTAAATGCACGGTTATCACGGTGGATACTTAGCTTTTGCAAGCTTGAGAACAAAATTATTTCCATAGAAAGGATTTATCAGTACAGTCAAATCCCAAGTGAAGCTCCCCCAGTTATTGAGGACTCTCGTCCTCCAACTAGATGGCCAGAGAATGGAACAATTGAACTACATGATTTAAAGGTTCGTTACAAGGAAAATCTTCCCGTAGTGCTTCATGGGGTAACTTGCACTTTTCCTGGCGGAAAGAAGATTGGAATTGTTGGCCGTACTGGAAGTGGCAAATCCACCTTGATCCAGGCCTTATTTAGACTGATTGAACCAGCAGGTGGGAGGATCCTTATAGACAAAATAGATATTTCAACTATCATACCCCAGGATCCTACCTTATTCGAAGGAACTATTAGGGGGAATCTTGATCCCCTTCAAGAGCATTCGGATCATGAAGTTTGGCAGGCACTTGACAAATCTCAGCTTGGAGATGTAATCCGTAAAACAGAGCACAAGCTTGATTCACCAGTGCTAGAAAATGGGGATAATTGGAGCGTGGGGCAGCGACAACTTGTTTCTCTGGGTCGGGCATTGCTTAAACAGGCCAAAATACTGGTTCTTGATGAAGCAACAGCATCAGTTGATACGCAGACAGACAATCTCATCCAGAAAATTATTCGAACAGAGTTTAAGAACTGTACTGTGTGCACTATAGCGCATCGTATACCAACCGTCATTGACAGTGATCTAGTATTGGTTCTGAGTGATGGTCGAGTGGCAGAGTTTGATACTCCTCAACGGCTGTTAGAAGATAAATCGTCCATGTTTCTAAAATTGGTAACTGAGTATTCATCAAGGTCAACTGGTATACCGGATTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004888 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006873 GO:0006875 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008281 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0010118 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022804 GO:0022857 GO:0023052 GO:0030003 GO:0030004 GO:0030007 GO:0031090 GO:0032870 GO:0033993 GO:0038023 GO:0042221 GO:0042592 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0097305 GO:0097306 GO:0098588 GO:0098771 GO:0098805 GO:1901527 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1519

Amino Acids

168.36

Weight (kDa)

8.53

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	339 - 604	1.7e-29	ABC transporter transmembrane region
ABC_tran	PF00005	657 - 791	2e-18	ABC transporter
ABC_membrane	PF00664	967 - 1209	1.8e-29	ABC transporter transmembrane region
ABC_tran	PF00005	1301 - 1438	3.2e-22	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04120 AT1G04120 AT1G04120
fragaria_vesca	FvH4_2g20350 FvH4_2g20350 FvH4_2g20350
malus_domestica	MD05G1187600.v1.1 MD10G1175300.v1.1
prunus_persica	Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1
pyrus_communis	pycom05g17240 pycom10g15100
rosa_chinensis	RchiOBHm_Chr6g0286471
rosa_laevigata	RLG00000012569
rosa_multiflora	Rmu_sc0034370.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182310
rosa_rugosa	Rorug06G0179900 Rorug06G0180000
rosa_samantha	Rh6AG292200 Rh6BG295800 Rh6CG296500 Rh6DG288300
rosa_wichuraiana	Rw6G025200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	906
AarI	CACCTGC	2 cut(s)	1751, 4004
Acc36I	ACCTGC	2 cut(s)	1751, 4004
AccB7I	CCANNNNNTGG	2 cut(s)	3842, 4016
AccI	GTMKAC	3 cut(s)	1239, 4402, 4546
AciI	CCGC	9 cut(s)	178, 500, 848, 1558, 2788, 3246, 3455, 3662, 3930
AclI	AACGTT	1 cut(s)	1967
AcoI	YGGCCR	6 cut(s)	501, 1518, 2299, 3456, 3833, 3952
AcsI	RAATTY	7 cut(s)	305, 916, 1569, 1816, 2287, 2471, 3688
AcuI	CTGAAG	4 cut(s)	963, 2356, 2733, 3140
AfaI	GTAC	9 cut(s)	594, 1729, 1747, 2107, 2198, 2811, 3773, 3958, 4378
AflII	CTTAAG	2 cut(s)	758, 1574
AflIII	ACRYGT	1 cut(s)	2311
AhlI	ACTAGT	1 cut(s)	2575
AjiI	CACGTC	1 cut(s)	1911
AjuI	GAANNNNNNNTTGG	4 cut(s)	206, 238, 2219, 2251
AleI	CACNNNNGTG	1 cut(s)	1287
AloI	GAACNNNNNNTCC	4 cut(s)	3473, 3505, 3634, 3666
Alw21I	GWGCWC	6 cut(s)	566, 1697, 3050, 3148, 4191, 4388
Alw26I	GTCTC	7 cut(s)	11, 547, 838, 1094, 1154, 1757, 2584
Alw44I	GTGCAC	3 cut(s)	562, 3144, 4384
AlwNI	CAGNNNCTG	1 cut(s)	2561
ApaLI	GTGCAC	3 cut(s)	562, 3144, 4384
ApoI	RAATTY	7 cut(s)	305, 916, 1569, 1816, 2287, 2471, 3688
ArsI	GACNNNNNNTTYG	2 cut(s)	64, 96
Asp700I	GAANNNNTTC	3 cut(s)	1423, 2163, 3888
AspLEI	GCGC	3 cut(s)	142, 2517, 4396
AspS9I	GGNCC	9 cut(s)	172, 196, 652, 1055, 1134, 1945, 2738, 3010, 3031
AsuC2I	CCSGG	2 cut(s)	175, 1174
AsuHPI	GGTGA	5 cut(s)	2099, 2285, 2579, 3449, 4194
AsuII	TTCGAA	3 cut(s)	230, 4080, 4357
AvaII	GGWCC	5 cut(s)	1055, 1134, 1945, 2738, 3010
BaeGI	GKGCMC	5 cut(s)	296, 566, 2332, 3148, 4388
BaeI	ACNNNNGTAYC	2 cut(s)	1719, 1752
BalI	TGGCCA	2 cut(s)	2301, 3835
BamHI	GGATCC	3 cut(s)	1794, 4023, 4066
BanII	GRGCYC	2 cut(s)	1697, 3050
BarI	GAAGNNNNNNTAC	2 cut(s)	591, 623
BbsI	GAAGAC	3 cut(s)	111, 1547, 2442
Bbv12I	GWGCWC	6 cut(s)	566, 1697, 3050, 3148, 4191, 4388
BceAI	ACGGC	4 cut(s)	1245, 1533, 3939, 4493
BciVI	GTATCC	2 cut(s)	2130, 3708
BclI	TGATCA	3 cut(s)	1065, 2641, 3205
BcnI	CCSGG	2 cut(s)	175, 1174
BcoDI	GTCTC	7 cut(s)	11, 547, 838, 1094, 1154, 1757, 2584
BcuI	ACTAGT	1 cut(s)	2575
BfaI	CTAG	9 cut(s)	143, 656, 1154, 2576, 2943, 3095, 3828, 4211, 4427
BfmI	CTRYAG	4 cut(s)	1758, 2871, 3541, 4389
BfoI	RGCGCY	1 cut(s)	143
BfrI	CTTAAG	2 cut(s)	758, 1574
BfuAI	ACCTGC	2 cut(s)	1751, 4004
BfuI	GTATCC	2 cut(s)	2130, 3708
BlpI	GCTNAGC	3 cut(s)	60, 451, 1267
Bme18I	GGWCC	5 cut(s)	1055, 1134, 1945, 2738, 3010
BmgBI	CACGTC	1 cut(s)	1911
BmgT120I	GGNCC	9 cut(s)	172, 196, 652, 1055, 1134, 1945, 2738, 3010, 3031
BmiI	GGNNCC	9 cut(s)	239, 1056, 1135, 1796, 1946, 1947, 3643, 4025, 4068
BmrI	ACTGGG	1 cut(s)	3794
BmuI	ACTGGG	1 cut(s)	3794
BoxI	GACNNNNGTC	2 cut(s)	2576, 3816
BpiI	GAAGAC	3 cut(s)	111, 1547, 2442
BpmI	CTGGAG	2 cut(s)	131, 1711
Bpu10I	CCTNAGC	1 cut(s)	3128
Bpu1102I	GCTNAGC	3 cut(s)	60, 451, 1267
Bpu14I	TTCGAA	3 cut(s)	230, 4080, 4357
BpuEI	CTTGAG	3 cut(s)	872, 968, 3755
BpuMI	CCSGG	2 cut(s)	175, 1174
Bsa29I	ATCGAT	1 cut(s)	889
BsaAI	YACGTR	1 cut(s)	1051
BsaI	GGTCTC	3 cut(s)	11, 838, 1154
BsaJI	CCNNGG	4 cut(s)	972, 1521, 2062, 4062
BsaWI	WCCGGW	2 cut(s)	2084, 4549
BsaXI	ACNNNNNCTCC	8 cut(s)	378, 408, 1284, 1314, 1625, 1655, 1880, 1910
Bse118I	RCCGGY	2 cut(s)	649, 1025
Bse1I	ACTGG	7 cut(s)	697, 2398, 3800, 3964, 4205, 4296, 4546
Bse3DI	GCAATG	4 cut(s)	515, 731, 1239, 4268
BseCI	ATCGAT	1 cut(s)	889
BseDI	CCNNGG	4 cut(s)	972, 1521, 2062, 4062
BseMI	GCAATG	4 cut(s)	515, 731, 1239, 4268
BseNI	ACTGG	7 cut(s)	697, 2398, 3800, 3964, 4205, 4296, 4546
BseRI	GAGGAG	4 cut(s)	399, 402, 1631, 4461
BseSI	GKGCMC	5 cut(s)	296, 566, 2332, 3148, 4388
BseX3I	CGGCCG	1 cut(s)	501
BseYI	CCCAGC	4 cut(s)	55, 782, 1130, 1941
BsgI	GTGCAG	5 cut(s)	426, 1343, 2336, 2397, 3087
Bsh1285I	CGRYCG	1 cut(s)	504
BshVI	ATCGAT	1 cut(s)	889
BsiEI	CGRYCG	1 cut(s)	504
BsiHKAI	GWGCWC	6 cut(s)	566, 1697, 3050, 3148, 4191, 4388
BsiSI	CCGG	7 cut(s)	175, 199, 650, 1026, 1174, 2085, 4550
BslFI	GGGAC	4 cut(s)	1147, 1263, 1349, 1958
BsmAI	GTCTC	7 cut(s)	11, 547, 838, 1094, 1154, 1757, 2584
BsmBI	CGTCTC	1 cut(s)	1757
BsmFI	GGGAC	4 cut(s)	1147, 1263, 1349, 1958
BsmI	GAATGC	2 cut(s)	3405, 4120
Bso31I	GGTCTC	3 cut(s)	11, 838, 1154
Bsp119I	TTCGAA	3 cut(s)	230, 4080, 4357
Bsp1286I	GDGCHC	8 cut(s)	296, 566, 1697, 2332, 3050, 3148, 4191, 4388
Bsp1407I	TGTACA	2 cut(s)	592, 2809
Bsp1720I	GCTNAGC	3 cut(s)	60, 451, 1267
BspACI	CCGC	9 cut(s)	178, 500, 848, 1558, 2788, 3246, 3455, 3662, 3930
BspDI	ATCGAT	1 cut(s)	889
BspHI	TCATGA	2 cut(s)	3492, 4129
BspLI	GGNNCC	9 cut(s)	239, 1056, 1135, 1796, 1946, 1947, 3643, 4025, 4068
BspMAI	CTGCAG	3 cut(s)	1762, 2875, 3545
BspMI	ACCTGC	2 cut(s)	1751, 4004
BspT104I	TTCGAA	3 cut(s)	230, 4080, 4357
BspTI	CTTAAG	2 cut(s)	758, 1574
BspTNI	GGTCTC	3 cut(s)	11, 838, 1154
BsrDI	GCAATG	4 cut(s)	515, 731, 1239, 4268
BsrFI	RCCGGY	2 cut(s)	649, 1025
BsrGI	TGTACA	2 cut(s)	592, 2809
BsrI	ACTGG	7 cut(s)	697, 2398, 3800, 3964, 4205, 4296, 4546
BssAI	RCCGGY	2 cut(s)	649, 1025
BssECI	CCNNGG	4 cut(s)	972, 1521, 2062, 4062
BssNAI	GTATAC	3 cut(s)	1240, 4403, 4547
BssT1I	CCWWGG	2 cut(s)	1521, 2062
Bst1107I	GTATAC	3 cut(s)	1240, 4403, 4547
Bst6I	CTCTTC	6 cut(s)	164, 828, 876, 1887, 2811, 2830
BstAFI	CTTAAG	2 cut(s)	758, 1574
BstAPI	GCANNNNNTGC	4 cut(s)	100, 2314, 2384, 3316
BstAUI	TGTACA	2 cut(s)	592, 2809
BstBAI	YACGTR	1 cut(s)	1051
BstBI	TTCGAA	3 cut(s)	230, 4080, 4357
BstC8I	GCNNGC	9 cut(s)	1556, 1584, 1668, 2322, 2790, 3529, 3614, 3731, 4143
BstH2I	RGCGCY	1 cut(s)	143
BstHHI	GCGC	3 cut(s)	142, 2517, 4396
BstMAI	GTCTC	7 cut(s)	11, 547, 838, 1094, 1154, 1757, 2584
BstMCI	CGRYCG	1 cut(s)	504
BstNSI	RCATGY	2 cut(s)	1214, 3616
BstPAI	GACNNNNGTC	2 cut(s)	2576, 3816
BstSFI	CTRYAG	4 cut(s)	1758, 2871, 3541, 4389
BstSLI	GKGCMC	5 cut(s)	296, 566, 2332, 3148, 4388
BstV2I	GAAGAC	3 cut(s)	111, 1547, 2442
BstX2I	RGATCY	7 cut(s)	946, 1794, 2070, 3183, 3220, 4023, 4066
BstXI	CCANNNNNNTGG	2 cut(s)	3831, 4291
BstYI	RGATCY	7 cut(s)	946, 1794, 2070, 3183, 3220, 4023, 4066
BstZ17I	GTATAC	3 cut(s)	1240, 4403, 4547
BstZI	CGGCCG	1 cut(s)	501
Bsu15I	ATCGAT	1 cut(s)	889
BsuI	GTATCC	2 cut(s)	2130, 3708
BsuTUI	ATCGAT	1 cut(s)	889
BtrI	CACGTC	1 cut(s)	1911
BtsI	GCAGTG	3 cut(s)	1184, 1755, 2710
BtsIMutI	CAGTG	9 cut(s)	318, 1184, 1755, 2222, 2380, 2391, 2710, 4212, 4426
BveI	ACCTGC	2 cut(s)	1751, 4004
Cac8I	GCNNGC	9 cut(s)	1556, 1584, 1668, 2322, 2790, 3529, 3614, 3731, 4143
CaiI	CAGNNNCTG	1 cut(s)	2561
CciI	TCATGA	2 cut(s)	3492, 4129
CfoI	GCGC	3 cut(s)	142, 2517, 4396
Cfr10I	RCCGGY	2 cut(s)	649, 1025
Cfr13I	GGNCC	9 cut(s)	172, 196, 652, 1055, 1134, 1945, 2738, 3010, 3031
ClaI	ATCGAT	1 cut(s)	889
CsiI	ACCWGGT	1 cut(s)	1827
Csp6I	GTAC	9 cut(s)	593, 1728, 1746, 2106, 2197, 2810, 3772, 3957, 4377
CviQI	GTAC	9 cut(s)	593, 1728, 1746, 2106, 2197, 2810, 3772, 3957, 4377
DraI	TTTAAA	3 cut(s)	304, 3693, 3867
EaeI	YGGCCR	6 cut(s)	501, 1518, 2299, 3456, 3833, 3952
EagI	CGGCCG	1 cut(s)	501
Eam1104I	CTCTTC	6 cut(s)	164, 828, 876, 1887, 2811, 2830
EarI	CTCTTC	6 cut(s)	164, 828, 876, 1887, 2811, 2830
EciI	GGCGGA	2 cut(s)	193, 3945
Ecl136II	GAGCTC	2 cut(s)	1695, 3048
EclXI	CGGCCG	1 cut(s)	501
Eco130I	CCWWGG	2 cut(s)	1521, 2062
Eco147I	AGGCCT	1 cut(s)	3989
Eco24I	GRGCYC	2 cut(s)	1697, 3050
Eco31I	GGTCTC	3 cut(s)	11, 838, 1154
Eco47I	GGWCC	5 cut(s)	1055, 1134, 1945, 2738, 3010
Eco52I	CGGCCG	1 cut(s)	501
Eco53kI	GAGCTC	2 cut(s)	1695, 3048
Eco57I	CTGAAG	4 cut(s)	963, 2356, 2733, 3140
EcoICRI	GAGCTC	2 cut(s)	1695, 3048
EcoO109I	RGGNCCY	2 cut(s)	1134, 1945
EcoRI	GAATTC	2 cut(s)	916, 1569
EcoT14I	CCWWGG	2 cut(s)	1521, 2062
EcoT22I	ATGCAT	1 cut(s)	1212
EcoT38I	GRGCYC	2 cut(s)	1697, 3050
ErhI	CCWWGG	2 cut(s)	1521, 2062
Esp3I	CGTCTC	1 cut(s)	1757
FalI	AAGNNNNNCTT	6 cut(s)	1845, 1877, 2840, 2872, 3303, 3335
FaqI	GGGAC	4 cut(s)	1147, 1263, 1349, 1958
FauI	CCCGC	3 cut(s)	2781, 3239, 3655
FauNDI	CATATG	2 cut(s)	1361, 3679
FbaI	TGATCA	3 cut(s)	1065, 2641, 3205
FblI	GTMKAC	3 cut(s)	1239, 4402, 4546
FriOI	GRGCYC	2 cut(s)	1697, 3050
FspBI	CTAG	9 cut(s)	143, 656, 1154, 2576, 2943, 3095, 3828, 4211, 4427
GlaI	GCGC	3 cut(s)	141, 2516, 4395
GsaI	CCCAGC	4 cut(s)	59, 786, 1134, 1945
GsuI	CTGGAG	2 cut(s)	131, 1711
HaeII	RGCGCY	1 cut(s)	143
HapII	CCGG	7 cut(s)	175, 199, 650, 1026, 1174, 2085, 4550
HhaI	GCGC	3 cut(s)	142, 2517, 4396
Hin6I	GCGC	3 cut(s)	140, 2515, 4394
HinP1I	GCGC	3 cut(s)	140, 2515, 4394
HincII	GTYRAC	2 cut(s)	1303, 4539
HindII	GTYRAC	2 cut(s)	1303, 4539
HindIII	AAGCTT	4 cut(s)	1584, 1668, 3731, 4193
HpaI	GTTAAC	1 cut(s)	1303
HpaII	CCGG	7 cut(s)	175, 199, 650, 1026, 1174, 2085, 4550
HphI	GGTGA	5 cut(s)	2099, 2285, 2579, 3449, 4194
HpyCH4IV	ACGT	4 cut(s)	1050, 1910, 1967, 2311
HpySE526I	ACGT	4 cut(s)	1050, 1910, 1967, 2311
HspAI	GCGC	3 cut(s)	140, 2515, 4394
KflI	GGGWCCC	1 cut(s)	1945
Ksp22I	TGATCA	3 cut(s)	1065, 2641, 3205
KspAI	GTTAAC	1 cut(s)	1303
LmnI	GCTCC	6 cut(s)	86, 112, 1692, 3045, 3799, 4230
MabI	ACCWGGT	1 cut(s)	1827
MaeI	CTAG	9 cut(s)	143, 656, 1154, 2576, 2943, 3095, 3828, 4211, 4427
MaeII	ACGT	4 cut(s)	1050, 1910, 1967, 2311
MfeI	CAATTG	5 cut(s)	2598, 3201, 3339, 3444, 3849
MflI	RGATCY	7 cut(s)	946, 1794, 2070, 3183, 3220, 4023, 4066
MhlI	GDGCHC	8 cut(s)	296, 566, 1697, 2332, 3050, 3148, 4191, 4388
MlsI	TGGCCA	2 cut(s)	2301, 3835
MluNI	TGGCCA	2 cut(s)	2301, 3835
MlyI	GAGTC	5 cut(s)	1245, 2630, 2847, 3449, 3806
MmeI	TCCRAC	4 cut(s)	655, 1396, 2650, 3848
Mox20I	TGGCCA	2 cut(s)	2301, 3835
Mph1103I	ATGCAT	1 cut(s)	1212
MroXI	GAANNNNTTC	3 cut(s)	1423, 2163, 3888
MscI	TGGCCA	2 cut(s)	2301, 3835
MslI	CAYNNNNRTG	6 cut(s)	255, 621, 1287, 1364, 1682, 2592
Msp20I	TGGCCA	2 cut(s)	2301, 3835
MspA1I	CMGCKG	2 cut(s)	440, 1715
MspCI	CTTAAG	2 cut(s)	758, 1574
MspI	CCGG	7 cut(s)	175, 199, 650, 1026, 1174, 2085, 4550
MunI	CAATTG	5 cut(s)	2598, 3201, 3339, 3444, 3849
Mva1269I	GAATGC	2 cut(s)	3405, 4120
NciI	CCSGG	2 cut(s)	175, 1174
NdeI	CATATG	2 cut(s)	1361, 3679
NlaIV	GGNNCC	9 cut(s)	239, 1056, 1135, 1796, 1946, 1947, 3643, 4025, 4068
NmeAIII	GCCGAG	2 cut(s)	455, 529
NmuCI	GTSAC	5 cut(s)	643, 688, 1280, 1717, 2455
NsiI	ATGCAT	1 cut(s)	1212
NspI	RCATGY	2 cut(s)	1214, 3616
NspV	TTCGAA	3 cut(s)	230, 4080, 4357
OliI	CACNNNNGTG	1 cut(s)	1287
PaeI	GCATGC	1 cut(s)	3616
PagI	TCATGA	2 cut(s)	3492, 4129
PaqCI	CACCTGC	2 cut(s)	1751, 4004
PceI	AGGCCT	1 cut(s)	3989
PctI	GAATGC	2 cut(s)	3405, 4120
PdmI	GAANNNNTTC	3 cut(s)	1423, 2163, 3888
PfeI	GAWTC	9 cut(s)	273, 1878, 2893, 2968, 3164, 3190, 3502, 4099, 4199
PflMI	CCANNNNNTGG	2 cut(s)	3842, 4016
PfoI	TCCNGGA	2 cut(s)	108, 276
PleI	GAGTC	5 cut(s)	1245, 2630, 2846, 3448, 3806
PpsI	GAGTC	5 cut(s)	1245, 2630, 2846, 3448, 3806
Ppu21I	YACGTR	1 cut(s)	1051
PpuMI	RGGWCCY	2 cut(s)	1134, 1945
PshAI	GACNNNNGTC	2 cut(s)	2576, 3816
PsiI	TTATAA	1 cut(s)	906
Psp124BI	GAGCTC	2 cut(s)	1697, 3050
Psp1406I	AACGTT	1 cut(s)	1967
Psp5II	RGGWCCY	2 cut(s)	1134, 1945
PspFI	CCCAGC	4 cut(s)	55, 782, 1130, 1941
PspN4I	GGNNCC	9 cut(s)	239, 1056, 1135, 1796, 1946, 1947, 3643, 4025, 4068
PspPI	GGNCC	9 cut(s)	172, 196, 652, 1055, 1134, 1945, 2738, 3010, 3031
PspPPI	RGGWCCY	2 cut(s)	1134, 1945
PstI	CTGCAG	3 cut(s)	1762, 2875, 3545
PstNI	CAGNNNCTG	1 cut(s)	2561
PsuI	RGATCY	7 cut(s)	946, 1794, 2070, 3183, 3220, 4023, 4066
PvuII	CAGCTG	2 cut(s)	440, 1715
RsaI	GTAC	9 cut(s)	594, 1729, 1747, 2107, 2198, 2811, 3773, 3958, 4378
RsaNI	GTAC	9 cut(s)	593, 1728, 1746, 2106, 2197, 2810, 3772, 3957, 4377
RseI	CAYNNNNRTG	6 cut(s)	255, 621, 1287, 1364, 1682, 2592
SacI	GAGCTC	2 cut(s)	1697, 3050
Sau96I	GGNCC	9 cut(s)	172, 196, 652, 1055, 1134, 1945, 2738, 3010, 3031
SchI	GAGTC	5 cut(s)	1245, 2630, 2847, 3449, 3806
SduI	GDGCHC	8 cut(s)	296, 566, 1697, 2332, 3050, 3148, 4191, 4388
SexAI	ACCWGGT	1 cut(s)	1827
SfcI	CTRYAG	4 cut(s)	1758, 2871, 3541, 4389
SfuI	TTCGAA	3 cut(s)	230, 4080, 4357
SinI	GGWCC	5 cut(s)	1055, 1134, 1945, 2738, 3010
SmiI	ATTTAAAT	1 cut(s)	3693
SmiMI	CAYNNNNRTG	6 cut(s)	255, 621, 1287, 1364, 1682, 2592
SmlI	CTYRAG	5 cut(s)	758, 851, 983, 1574, 3734
SmoI	CTYRAG	5 cut(s)	758, 851, 983, 1574, 3734
SpeI	ACTAGT	1 cut(s)	2575
SphI	GCATGC	1 cut(s)	3616
SseBI	AGGCCT	1 cut(s)	3989
SsiI	CCGC	9 cut(s)	178, 500, 848, 1558, 2788, 3246, 3455, 3662, 3930
SspI	AATATT	2 cut(s)	2164, 2922
SspMI	CTAG	9 cut(s)	143, 656, 1154, 2576, 2943, 3095, 3828, 4211, 4427
SstI	GAGCTC	2 cut(s)	1697, 3050
StuI	AGGCCT	1 cut(s)	3989
StyI	CCWWGG	2 cut(s)	1521, 2062
SwaI	ATTTAAAT	1 cut(s)	3693
TaiI	ACGT	4 cut(s)	1053, 1913, 1970, 2314
TaqI	TCGA	8 cut(s)	230, 605, 889, 2469, 3590, 4080, 4357, 4450
TatI	WGTACW	5 cut(s)	592, 2196, 2809, 3771, 4376
TauI	GCSGC	2 cut(s)	503, 3458
TfiI	GAWTC	9 cut(s)	273, 1878, 2893, 2968, 3164, 3190, 3502, 4099, 4199
TscAI	CASTG	9 cut(s)	318, 1184, 1762, 2229, 2380, 2398, 2710, 4212, 4426
TseFI	GTSAC	5 cut(s)	643, 688, 1280, 1717, 2455
Tsp45I	GTSAC	5 cut(s)	643, 688, 1280, 1717, 2455
TspGWI	ACGGA	5 cut(s)	1047, 3131, 3196, 3589, 4166
TspRI	CASTG	9 cut(s)	318, 1184, 1762, 2229, 2380, 2398, 2710, 4212, 4426
Van91I	CCANNNNNTGG	2 cut(s)	3842, 4016
Vha464I	CTTAAG	2 cut(s)	758, 1574
VneI	GTGCAC	3 cut(s)	562, 3144, 4384
VpaK11BI	GGWCC	5 cut(s)	1055, 1134, 1945, 2738, 3010
XapI	RAATTY	7 cut(s)	305, 916, 1569, 1816, 2287, 2471, 3688
XbaI	TCTAGA	1 cut(s)	3094
XceI	RCATGY	2 cut(s)	1214, 3616
XmiI	GTMKAC	3 cut(s)	1239, 4402, 4546
XmnI	GAANNNNTTC	3 cut(s)	1423, 2163, 3888
XspI	CTAG	9 cut(s)	143, 656, 1154, 2576, 2943, 3095, 3828, 4211, 4427
Zsp2I	ATGCAT	1 cut(s)	1212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.