Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006477

Physical Location & Seq

Genomic Coordinates Reverse (-)

34091775 .. 34096063

Gene Structure 4289 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1209300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGAGATTACCAACGTCAGCGAGTATGAGGCCATCGCAAAGGAGAAGTTGCCAAAGATGGTCTATGACTACTATGCATCAGGAGCAGAGGACCAGTGGACTCTTGCTGAAAACAGAAATGCTTTCGCGAGAATTCTGTTTCGACCCCGTATTCTCATTGATGTGAGCCGCATAGACATGACCACCACTGTTTTGGGCTTCAAGATCTCAATGCCCATCATGATTGCCCCAACAGCCATGCAGAAAATGGCCCATCCTGAAGGTGAATATGCTACTGCGAGGGCAGCATCAGCTGCAGGAACAATTATGACCTTATCTTCATGGGCTACTTCAAGTGTTGAAGAGGTTGCTTCAACTGGACCTGGAATCCGTTTCTTCCAGCTCTATGTCTACAAGGACAGGAATGTGGTTGCTCAGCTGGTGAGAAGAGCTGAAAGGGCTGGGTTCAAGGCTATTGCTCTTACAGTTGATACCCCAAGGCTCGGTCGCAGAGAAGCTGACATCAAGAACAGATTTACCTTGCCTCCGTTTTTGACACTGAAGAACTTTGAGGGTTTGGACCTCGGAAAGATGGACAAAGCTGCTGACTCTGGACTTGCTTCATACGTTGCTGGTCAAATCGATCGTTCTCTTAGCTGGAAGGATGTGCAGTGGCTTCAGACAATCACTTCACTGCCTATCCTAGTTAAGGGTGTTCTCACTGCCGAAGATGCAAGGATTGCAGTACAAGCTGGAGCAGCTGGGATTATTGTGTCCAACCATGGAGCTCGCCAACTTGATTACGTCCCTTCGACTATCATGGCCCTCGAAGAGGTTGTTAAAGGTGCACAAGGACGCATTCCTGTTTTCTTGGATGGTGGTGTCCGTCGAGGAACAGATGTCTTCAAAGCATTAGCTTTGGGTGCCTCTGGCATATTTATTGGGCGCCCTGTGGTGTTCTCTCTCGCTGCTGAAGGAGAGGCTGGCATTAGAAAGGTACTCCAAATGCTGCGCGAAGAGTTTGAGCTAACCATGGCATTAAGTGGATGCCGTTCGCTCAAAGAGATCACCCGCGATCACATTGTGACTGACTGGGACGCTCCTCGCCCTCGCCATCAGCCAAGGTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003973 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005829 GO:0005840 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008891 GO:0009058 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0016020 GO:0016491 GO:0016614 GO:0016899 GO:0016999 GO:0017000 GO:0017144 GO:0022626 GO:0030054 GO:0032991 GO:0042221 GO:0042579 GO:0042742 GO:0042743 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044249 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048046 GO:0050665 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055114 GO:0072593 GO:0098542 GO:1903409 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko04146 map00630 map01100 map01110 map01120 map01130 map01200 map04146

Pfam Domains

Protein Families

FMN_dh

Protein Analysis

370

Amino Acids

40.58

Weight (kDa)

9.17

Isoelectric Point (pI)

33.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FMN_dh	PF01070	13 - 355	8.8e-138	FMN-dependent dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14415 AT3G14415 AT3G14415 AT3G14420 AT3G14420 AT3G14420 AT3G14420 AT3G14420 AT3G14420
fragaria_vesca	FvH4_3g16520
malus_domestica	MD05G1209300.v1.1 MD10G1195700.v1.1
prunus_persica	Prupe.4G148200_v2.0.a1 Prupe.4G148200_v2.0.a1
pyrus_communis	pycom05g19540 pycom10g16930
rosa_chinensis	RchiOBHm_Chr5g0027571
rosa_laevigata	RLG00000033027
rosa_multiflora	Rmu_sc0001647.1_g000002 Rmu_sc0011056.1_g000007
rosa_roxburghii	Rroxscaffold_1G00051970
rosa_rugosa	Rorug05G0099800 Rorug05G0099800
rosa_samantha	Rh5AG194000 Rh5BG192000
rosa_wichuraiana	Rw5G017690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	902, 924
AccI	GTMKAC	1 cut(s)	390
AccII	CGCG	3 cut(s)	128, 993, 1053
AciI	CCGC	2 cut(s)	169, 1051
AcsI	RAATTY	1 cut(s)	132
AcuI	CTGAAG	4 cut(s)	279, 560, 641, 972
AcyI	GRCGYC	1 cut(s)	925
AdeI	CACNNNGTG	1 cut(s)	1063
AfaI	GTAC	2 cut(s)	726, 978
AfiI	CCNNNNNNNGG	3 cut(s)	482, 688, 811
AgsI	TTSAA	6 cut(s)	202, 333, 341, 354, 448, 886
AjnI	CCWGG	1 cut(s)	361
Alw21I	GWGCWC	2 cut(s)	769, 829
Alw44I	GTGCAC	1 cut(s)	825
AoxI	GGCC	3 cut(s)	30, 249, 801
ApaLI	GTGCAC	1 cut(s)	825
ApeKI	GCWGC	6 cut(s)	284, 293, 581, 737, 947, 988
ApoI	RAATTY	1 cut(s)	132
AspLEI	GCGC	2 cut(s)	927, 993
AspS9I	GGNCC	5 cut(s)	91, 250, 359, 559, 802
AsuHPI	GGTGA	3 cut(s)	275, 433, 1039
AvaII	GGWCC	3 cut(s)	91, 359, 559
BaeGI	GKGCMC	1 cut(s)	829
BanI	GGYRCC	2 cut(s)	902, 924
BanII	GRGCYC	1 cut(s)	769
BbsI	GAAGAC	1 cut(s)	874
Bbv12I	GWGCWC	2 cut(s)	769, 829
BbvI	GCAGC	6 cut(s)	280, 296, 568, 749, 934, 975
BccI	CCATC	7 cut(s)	41, 52, 224, 261, 565, 848, 1101
BceAI	ACGGC	1 cut(s)	1014
BciT130I	CCWGG	1 cut(s)	363
BfaI	CTAG	1 cut(s)	683
BfmI	CTRYAG	1 cut(s)	294
BfoI	RGCGCY	1 cut(s)	928
BglII	AGATCT	1 cut(s)	204
BisI	GCNGC	7 cut(s)	169, 285, 294, 582, 738, 948, 989
BlpI	GCTNAGC	1 cut(s)	414
BlsI	GCNGC	7 cut(s)	170, 286, 295, 583, 739, 949, 990
Bme1390I	CCNGG	1 cut(s)	363
Bme18I	GGWCC	3 cut(s)	91, 359, 559
BmgT120I	GGNCC	5 cut(s)	91, 250, 359, 559, 802
BmiI	GGNNCC	2 cut(s)	904, 926
BmrFI	CCNGG	1 cut(s)	363
BmrI	ACTGGG	1 cut(s)	1081
BmsI	GCATC	4 cut(s)	86, 296, 700, 1016
BmuI	ACTGGG	1 cut(s)	1081
BpiI	GAAGAC	1 cut(s)	874
BpmI	CTGGAG	1 cut(s)	753
Bpu1102I	GCTNAGC	1 cut(s)	414
Bsa29I	ATCGAT	1 cut(s)	621
BsaHI	GRCGYC	1 cut(s)	925
BsaJI	CCNNGG	5 cut(s)	476, 562, 760, 1011, 1100
BsaXI	ACNNNNNCTCC	2 cut(s)	508, 538
Bsc4I	CCNNNNNNNGG	3 cut(s)	482, 688, 811
Bse1I	ACTGG	3 cut(s)	94, 361, 1076
BseBI	CCWGG	1 cut(s)	363
BseCI	ATCGAT	1 cut(s)	621
BseDI	CCNNGG	5 cut(s)	476, 562, 760, 1011, 1100
BseGI	GGATG	4 cut(s)	253, 649, 859, 1031
BseLI	CCNNNNNNNGG	3 cut(s)	482, 688, 811
BseMII	CTCAG	1 cut(s)	428
BseNI	ACTGG	3 cut(s)	94, 361, 1076
BseRI	GAGGAG	1 cut(s)	1071
BseSI	GKGCMC	1 cut(s)	829
BseXI	GCAGC	6 cut(s)	280, 296, 568, 749, 934, 975
BseYI	CCCAGC	2 cut(s)	440, 740
BsgI	GTGCAG	1 cut(s)	668
Bsh1236I	CGCG	3 cut(s)	128, 993, 1053
Bsh1285I	CGRYCG	2 cut(s)	487, 625
BshFI	GGCC	3 cut(s)	32, 251, 803
BshNI	GGYRCC	2 cut(s)	902, 924
BshVI	ATCGAT	1 cut(s)	621
BsiEI	CGRYCG	2 cut(s)	487, 625
BsiHKAI	GWGCWC	2 cut(s)	769, 829
BslFI	GGGAC	2 cut(s)	770, 1088
BslI	CCNNNNNNNGG	3 cut(s)	482, 688, 811
BsmFI	GGGAC	2 cut(s)	770, 1088
BsmI	GAATGC	1 cut(s)	837
BsnI	GGCC	3 cut(s)	32, 251, 803
Bsp1286I	GDGCHC	2 cut(s)	769, 829
Bsp143I	GATC	4 cut(s)	204, 622, 1044, 1054
Bsp1720I	GCTNAGC	1 cut(s)	414
Bsp19I	CCATGG	2 cut(s)	760, 1011
Bsp68I	TCGCGA	1 cut(s)	128
BspACI	CCGC	2 cut(s)	169, 1051
BspANI	GGCC	3 cut(s)	32, 251, 803
BspCNI	CTCAG	1 cut(s)	427
BspDI	ATCGAT	1 cut(s)	621
BspFNI	CGCG	3 cut(s)	128, 993, 1053
BspHI	TCATGA	1 cut(s)	219
BspLI	GGNNCC	2 cut(s)	904, 926
BspMAI	CTGCAG	1 cut(s)	298
BspQI	GCTCTTC	1 cut(s)	421
BspT107I	GGYRCC	2 cut(s)	902, 924
BsrI	ACTGG	3 cut(s)	94, 361, 1076
BssECI	CCNNGG	5 cut(s)	476, 562, 760, 1011, 1100
BssMI	GATC	4 cut(s)	204, 622, 1044, 1054
BssNI	GRCGYC	1 cut(s)	925
BssT1I	CCWWGG	4 cut(s)	476, 760, 1011, 1100
Bst2UI	CCWGG	1 cut(s)	363
Bst4CI	ACNGT	2 cut(s)	190, 466
Bst6I	CTCTTC	4 cut(s)	336, 421, 804, 990
BstACI	GRCGYC	1 cut(s)	925
BstAPI	GCANNNNNTGC	2 cut(s)	293, 719
BstC8I	GCNNGC	2 cut(s)	769, 964
BstDEI	CTNAG	2 cut(s)	414, 632
BstDSI	CCRYGG	2 cut(s)	760, 1011
BstENI	CCTNNNNNAGG	2 cut(s)	686, 809
BstF5I	GGATG	4 cut(s)	253, 649, 859, 1031
BstFNI	CGCG	3 cut(s)	128, 993, 1053
BstH2I	RGCGCY	1 cut(s)	928
BstHHI	GCGC	2 cut(s)	927, 993
BstKTI	GATC	4 cut(s)	207, 625, 1047, 1057
BstMBI	GATC	4 cut(s)	204, 622, 1044, 1054
BstMCI	CGRYCG	2 cut(s)	487, 625
BstNI	CCWGG	1 cut(s)	363
BstSCI	CCNGG	1 cut(s)	361
BstSFI	CTRYAG	1 cut(s)	294
BstSLI	GKGCMC	1 cut(s)	829
BstUI	CGCG	3 cut(s)	128, 993, 1053
BstV1I	GCAGC	6 cut(s)	280, 296, 568, 749, 934, 975
BstV2I	GAAGAC	1 cut(s)	874
BstX2I	RGATCY	1 cut(s)	204
BstXI	CCANNNNNNTGG	1 cut(s)	193
BstYI	RGATCY	1 cut(s)	204
Bsu15I	ATCGAT	1 cut(s)	621
BsuRI	GGCC	3 cut(s)	32, 251, 803
BsuTUI	ATCGAT	1 cut(s)	621
BtgI	CCRYGG	2 cut(s)	760, 1011
BtgZI	GCGATG	1 cut(s)	19
BtsCI	GGATG	4 cut(s)	253, 649, 859, 1031
BtsI	GCAGTG	3 cut(s)	656, 671, 699
BtsIMutI	CAGTG	6 cut(s)	101, 186, 536, 656, 671, 699
BtuMI	TCGCGA	1 cut(s)	128
Cac8I	GCNNGC	2 cut(s)	769, 964
CciI	TCATGA	1 cut(s)	219
CfoI	GCGC	2 cut(s)	927, 993
Cfr13I	GGNCC	5 cut(s)	91, 250, 359, 559, 802
ClaI	ATCGAT	1 cut(s)	621
CseI	GACGC	2 cut(s)	843, 1085
Csp6I	GTAC	2 cut(s)	725, 977
CviAII	CATG	7 cut(s)	178, 220, 238, 321, 761, 799, 1012
CviQI	GTAC	2 cut(s)	725, 977
DdeI	CTNAG	2 cut(s)	414, 632
DinI	GGCGCC	1 cut(s)	926
DpnI	GATC	4 cut(s)	206, 624, 1046, 1056
DpnII	GATC	4 cut(s)	204, 622, 1044, 1054
DraIII	CACNNNGTG	1 cut(s)	1063
Eam1104I	CTCTTC	4 cut(s)	336, 421, 804, 990
EarI	CTCTTC	4 cut(s)	336, 421, 804, 990
Ecl136II	GAGCTC	1 cut(s)	767
Eco130I	CCWWGG	4 cut(s)	476, 760, 1011, 1100
Eco24I	GRGCYC	1 cut(s)	769
Eco47I	GGWCC	3 cut(s)	91, 359, 559
Eco53kI	GAGCTC	1 cut(s)	767
Eco57I	CTGAAG	4 cut(s)	279, 560, 641, 972
EcoICRI	GAGCTC	1 cut(s)	767
EcoNI	CCTNNNNNAGG	2 cut(s)	686, 809
EcoRI	GAATTC	1 cut(s)	132
EcoRII	CCWGG	1 cut(s)	361
EcoT14I	CCWWGG	4 cut(s)	476, 760, 1011, 1100
EcoT22I	ATGCAT	1 cut(s)	79
EcoT38I	GRGCYC	1 cut(s)	769
EgeI	GGCGCC	1 cut(s)	926
EheI	GGCGCC	1 cut(s)	926
ErhI	CCWWGG	4 cut(s)	476, 760, 1011, 1100
FaeI	CATG	7 cut(s)	181, 223, 241, 324, 764, 802, 1015
FaqI	GGGAC	2 cut(s)	770, 1088
FatI	CATG	7 cut(s)	177, 219, 237, 320, 760, 798, 1011
FauI	CCCGC	1 cut(s)	1058
FblI	GTMKAC	1 cut(s)	390
Fnu4HI	GCNGC	7 cut(s)	169, 285, 294, 582, 738, 948, 989
FokI	GGATG	4 cut(s)	240, 656, 866, 1038
FriOI	GRGCYC	1 cut(s)	769
Fsp4HI	GCNGC	7 cut(s)	169, 285, 294, 582, 738, 948, 989
FspBI	CTAG	1 cut(s)	683
GlaI	GCGC	2 cut(s)	926, 992
GluI	GCNGC	7 cut(s)	169, 285, 294, 582, 738, 948, 989
GsaI	CCCAGC	2 cut(s)	444, 744
GsuI	CTGGAG	1 cut(s)	753
HaeII	RGCGCY	1 cut(s)	928
HaeIII	GGCC	3 cut(s)	32, 251, 803
HgaI	GACGC	2 cut(s)	843, 1085
HhaI	GCGC	2 cut(s)	927, 993
Hin1I	GRCGYC	1 cut(s)	925
Hin1II	CATG	7 cut(s)	181, 223, 241, 324, 764, 802, 1015
Hin6I	GCGC	2 cut(s)	925, 991
HinP1I	GCGC	2 cut(s)	925, 991
HinfI	GANTC	3 cut(s)	100, 366, 587
HphI	GGTGA	3 cut(s)	275, 433, 1039
Hpy166II	GTNNAC	3 cut(s)	99, 391, 827
Hpy188I	TCNGA	2 cut(s)	566, 660
Hpy188III	TCNNGA	7 cut(s)	81, 127, 202, 220, 257, 505, 591
Hpy8I	GTNNAC	3 cut(s)	99, 391, 827
Hpy99I	CGWCG	1 cut(s)	870
HpyAV	CCTTC	4 cut(s)	254, 634, 798, 947
HpyCH4III	ACNGT	2 cut(s)	190, 466
HpyCH4IV	ACGT	3 cut(s)	15, 606, 783
HpyCH4V	TGCA	7 cut(s)	77, 241, 296, 649, 713, 722, 827
HpyF3I	CTNAG	2 cut(s)	414, 632
HpySE526I	ACGT	3 cut(s)	15, 606, 783
Hsp92I	GRCGYC	1 cut(s)	925
Hsp92II	CATG	7 cut(s)	181, 223, 241, 324, 764, 802, 1015
HspAI	GCGC	2 cut(s)	925, 991
KasI	GGCGCC	1 cut(s)	924
Kzo9I	GATC	4 cut(s)	204, 622, 1044, 1054
LguI	GCTCTTC	1 cut(s)	421
LmnI	GCTCC	4 cut(s)	83, 734, 764, 1084
Lsp1109I	GCAGC	6 cut(s)	280, 296, 568, 749, 934, 975
LweI	GCATC	4 cut(s)	86, 296, 700, 1016
MaeI	CTAG	1 cut(s)	683
MaeII	ACGT	3 cut(s)	15, 606, 783
MaeIII	GTNAC	1 cut(s)	1063
MalI	GATC	4 cut(s)	206, 624, 1046, 1056
MboI	GATC	4 cut(s)	204, 622, 1044, 1054
MboII	GAAGA	9 cut(s)	309, 353, 367, 438, 553, 719, 821, 874, 1007
MflI	RGATCY	1 cut(s)	204
MhlI	GDGCHC	2 cut(s)	769, 829
MluCI	AATT	2 cut(s)	132, 303
Mly113I	GGCGCC	1 cut(s)	925
MlyI	GAGTC	2 cut(s)	94, 581
MmeI	TCCRAC	1 cut(s)	780
Mph1103I	ATGCAT	1 cut(s)	79
MseI	TTAA	3 cut(s)	687, 819, 1019
MslI	CAYNNNNRTG	2 cut(s)	161, 176
MspA1I	CMGCKG	3 cut(s)	293, 418, 740
MspR9I	CCNGG	1 cut(s)	363
Mva1269I	GAATGC	1 cut(s)	837
MvaI	CCWGG	1 cut(s)	363
MvnI	CGCG	3 cut(s)	128, 993, 1053
NarI	GGCGCC	1 cut(s)	925
NcoI	CCATGG	2 cut(s)	760, 1011
NdeII	GATC	4 cut(s)	204, 622, 1044, 1054
NlaIII	CATG	7 cut(s)	181, 223, 241, 324, 764, 802, 1015
NlaIV	GGNNCC	2 cut(s)	904, 926
NmuCI	GTSAC	1 cut(s)	1063
NruI	TCGCGA	1 cut(s)	128
NsiI	ATGCAT	1 cut(s)	79
PagI	TCATGA	1 cut(s)	219
PciSI	GCTCTTC	1 cut(s)	421
PctI	GAATGC	1 cut(s)	837
PfeI	GAWTC	1 cut(s)	366
PkrI	GCNGC	7 cut(s)	170, 286, 295, 583, 739, 949, 990
Ple19I	CGATCG	1 cut(s)	625
PleI	GAGTC	2 cut(s)	94, 581
PluTI	GGCGCC	1 cut(s)	928
PpsI	GAGTC	2 cut(s)	94, 581
Psp124BI	GAGCTC	1 cut(s)	769
Psp6I	CCWGG	1 cut(s)	361
PspFI	CCCAGC	2 cut(s)	440, 740
PspGI	CCWGG	1 cut(s)	361
PspN4I	GGNNCC	2 cut(s)	904, 926
PspPI	GGNCC	5 cut(s)	91, 250, 359, 559, 802
PstI	CTGCAG	1 cut(s)	298
PsuI	RGATCY	1 cut(s)	204
PvuI	CGATCG	1 cut(s)	625
PvuII	CAGCTG	3 cut(s)	293, 418, 740
RruI	TCGCGA	1 cut(s)	128
RsaI	GTAC	2 cut(s)	726, 978
RsaNI	GTAC	2 cut(s)	725, 977
RseI	CAYNNNNRTG	2 cut(s)	161, 176
SacI	GAGCTC	1 cut(s)	769
SapI	GCTCTTC	1 cut(s)	421
SaqAI	TTAA	3 cut(s)	687, 819, 1019
SatI	GCNGC	7 cut(s)	169, 285, 294, 582, 738, 948, 989
Sau3AI	GATC	4 cut(s)	204, 622, 1044, 1054
Sau96I	GGNCC	5 cut(s)	91, 250, 359, 559, 802
SchI	GAGTC	2 cut(s)	94, 581
ScrFI	CCNGG	1 cut(s)	363
SduI	GDGCHC	2 cut(s)	769, 829
SfaNI	GCATC	4 cut(s)	86, 296, 700, 1016
SfcI	CTRYAG	1 cut(s)	294
SfoI	GGCGCC	1 cut(s)	926
SinI	GGWCC	3 cut(s)	91, 359, 559
SmiMI	CAYNNNNRTG	2 cut(s)	161, 176
Sse9I	AATT	2 cut(s)	132, 303
SsiI	CCGC	2 cut(s)	169, 1051
SspDI	GGCGCC	1 cut(s)	924
SspMI	CTAG	1 cut(s)	683
SstI	GAGCTC	1 cut(s)	769
StyD4I	CCNGG	1 cut(s)	361
StyI	CCWWGG	4 cut(s)	476, 760, 1011, 1100
TaaI	ACNGT	2 cut(s)	190, 466
TaiI	ACGT	3 cut(s)	18, 609, 786
TaqI	TCGA	5 cut(s)	142, 621, 791, 807, 868
TaqII	GACCGA	1 cut(s)	473
TasI	AATT	2 cut(s)	132, 303
TatI	WGTACW	1 cut(s)	724
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	1 cut(s)	366
Tru1I	TTAA	3 cut(s)	687, 819, 1019
Tru9I	TTAA	3 cut(s)	687, 819, 1019
TscAI	CASTG	6 cut(s)	101, 193, 543, 656, 678, 706
TseFI	GTSAC	1 cut(s)	1063
TseI	GCWGC	6 cut(s)	284, 293, 581, 737, 947, 988
Tsp45I	GTSAC	1 cut(s)	1063
TspDTI	ATGAA	2 cut(s)	309, 591
TspGWI	ACGGA	3 cut(s)	359, 516, 854
TspRI	CASTG	6 cut(s)	101, 193, 543, 656, 678, 706
VneI	GTGCAC	1 cut(s)	825
VpaK11BI	GGWCC	3 cut(s)	91, 359, 559
XagI	CCTNNNNNAGG	2 cut(s)	686, 809
XapI	RAATTY	1 cut(s)	132
XcmI	CCANNNNNNNNNTGG	2 cut(s)	190, 244
XmiI	GTMKAC	1 cut(s)	390
XspI	CTAG	1 cut(s)	683
Zsp2I	ATGCAT	1 cut(s)	79

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.