MD05G1222700.v1.1

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010186
Physical Location & Seq
Forward (+)
35525839 .. 35527355
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1222700.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGCGTCGGCGGCGCCGGTGAACAGGATCGAGCGGGCCCACCAGATGCACCGGGAAGGTAGCTACTCGGAGGCGCTAGGGTTTTACACCGAAGCCCTGTCCATGGCCAGGACCAAACCCCAGAAGATCGCCCTCCACAGCAACCGAGCTGCTTGCTTCTTGAAGCTTCACGATTTCAAAAAGGCAGCAGATGAATGTACCTCAGTTCTTGAGCTTGATTACAATCACACTGGAGCATTGATGTTGCGGGCGCAGACCCTGGTGACCCTCAAGGAATATCACTCGGCGCTCTTCGATGTTCATCGTCTTATCGAGCTAAATCCATCATCGGAAGTTTATCAAAACCTTCAAGCCCGTCTAAAGACACAAGTGTCACTTGCTCCAATACCTGAATCCGAAGCAGAGCTAGAAGAAGAGGAAGACGAGGAGGAGGATGAAGCAGAGCCAGATAGATATCAGGAGAAAGAGGAGCAATACGAAGAAAAAGAAGATGTATTTTCAGGTGGAGGAAGGGATCAGATTCCTGAGGTTAATGAGACAACTGCTGTTGCCAATGTTATTGCCCCTACGACACCAGTAAACAGGGAAGTTTCTCAACAGGGAAGGGATCCAAAAGGTAACTCGATGAAGACCACTTCCCCAGCTGAAGTTGTGACCCCTCGTGCAGAGCACGTCAAGGCATCGTCTGAAAAAGAAAAAGACAAAGGATGGCAAGCAATCCCGAAGCCAAAGGGACACTCTTCTCTGGACTATGCACGGTGGGACAGAGTGGAAGATGACTCTAGTGAAGATGATGACGATGAAGACTCTCAACCTCAGTATAGATTCCGTGTAAAAACTGTTGGTGTCCGGCCAGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

288

Amino Acids

32.44

Weight (kDa)

4.92

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 20 - 97 3.2e-06 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 34
AciI CCGC 3 cut(s) 11, 34, 247
AclWI GGATC 4 cut(s) 35, 522, 602, 615
AcoI YGGCCR 2 cut(s) 105, 851
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 2 cut(s) 5, 14
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 103, 108
AgsI TTSAA 3 cut(s) 163, 178, 350
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 2 cut(s) 107, 258
AluBI AGCT 7 cut(s) 63, 149, 166, 214, 316, 406, 644
AluI AGCT 7 cut(s) 63, 149, 166, 214, 316, 406, 644
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 4 cut(s) 35, 522, 602, 615
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 3 cut(s) 36, 105, 851
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 149, 185
AspLEI GCGC 4 cut(s) 16, 76, 253, 289
AspS9I GGNCC 3 cut(s) 36, 37, 111
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 31, 274
AvaII GGWCC 1 cut(s) 111
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 107
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 40
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 3 cut(s) 426, 635, 810
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 136, 197
BccI CCATC 2 cut(s) 331, 702
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 2 cut(s) 109, 260
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 3 cut(s) 77, 407, 783
BfoI RGCGCY 3 cut(s) 17, 77, 290
BisI GCNGC 3 cut(s) 12, 150, 186
BlsI GCNGC 3 cut(s) 13, 151, 187
Bme1390I CCNGG 3 cut(s) 53, 109, 260
Bme18I GGWCC 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 3 cut(s) 36, 37, 111
BmiI GGNNCC 3 cut(s) 15, 38, 609
BmrFI CCNGG 3 cut(s) 53, 109, 260
BmsI GCATC 2 cut(s) 36, 689
BpiI GAAGAC 3 cut(s) 426, 635, 810
BpmI CTGGAG 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 230, 254
BpuMI CCSGG 1 cut(s) 53
BsaBI GATNNNNATC 3 cut(s) 222, 300, 453
BsaHI GRCGYC 2 cut(s) 5, 14
BsaJI CCNNGG 2 cut(s) 102, 258
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 108
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 3 cut(s) 235, 575, 854
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 3 cut(s) 222, 300, 453
BseBI CCWGG 2 cut(s) 109, 260
BseDI CCNNGG 2 cut(s) 102, 258
BseGI GGATG 2 cut(s) 439, 713
BseJI GATNNNNATC 3 cut(s) 222, 300, 453
BseLI CCNNNNNNNGG 2 cut(s) 103, 108
BseMII CTCAG 3 cut(s) 216, 516, 830
BseNI ACTGG 3 cut(s) 235, 575, 854
BseRI GAGGAG 3 cut(s) 440, 443, 482
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 2 cut(s) 136, 197
BseYI CCCAGC 1 cut(s) 640
BsgI GTGCAG 1 cut(s) 684
BshFI GGCC 3 cut(s) 38, 107, 853
BshNI GGYRCC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 3 cut(s) 17, 52, 850
BslFI GGGAC 2 cut(s) 747, 776
BslI CCNNNNNNNGG 2 cut(s) 103, 108
BsmAI GTCTC 1 cut(s) 530
BsmFI GGGAC 2 cut(s) 747, 776
BsnI GGCC 3 cut(s) 38, 107, 853
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 40, 672
Bsp143I GATC 4 cut(s) 27, 126, 514, 607
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 3 cut(s) 11, 34, 247
BspANI GGCC 3 cut(s) 38, 107, 853
BspCNI CTCAG 3 cut(s) 215, 517, 829
BspLI GGNNCC 3 cut(s) 15, 38, 609
BspPI GGATC 4 cut(s) 35, 522, 602, 615
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 3 cut(s) 235, 575, 854
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 102, 258
BssMI GATC 4 cut(s) 27, 126, 514, 607
BssNI GRCGYC 2 cut(s) 5, 14
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 109, 260
Bst4CI ACNGT 2 cut(s) 759, 841
Bst6I CTCTTC 3 cut(s) 296, 408, 745
BstACI GRCGYC 2 cut(s) 5, 14
BstC8I GCNNGC 4 cut(s) 36, 154, 249, 714
BstDEI CTNAG 3 cut(s) 202, 525, 816
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 2 cut(s) 439, 713
BstH2I RGCGCY 3 cut(s) 17, 77, 290
BstHHI GCGC 4 cut(s) 16, 76, 253, 289
BstKTI GATC 4 cut(s) 30, 129, 517, 610
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 4 cut(s) 27, 126, 514, 607
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 109, 260
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 51, 107, 258
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 136, 197
BstV2I GAAGAC 3 cut(s) 426, 635, 810
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 3 cut(s) 38, 107, 853
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 2 cut(s) 439, 713
BtsIMutI CAGTG 2 cut(s) 228, 861
Cac8I GCNNGC 4 cut(s) 36, 154, 249, 714
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 4 cut(s) 16, 76, 253, 289
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 36, 37, 111
Csp6I GTAC 1 cut(s) 198
CviAII CATG 1 cut(s) 103
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 3 cut(s) 202, 525, 816
DinI GGCGCC 1 cut(s) 15
DpnI GATC 4 cut(s) 29, 128, 516, 609
DpnII GATC 4 cut(s) 27, 126, 514, 607
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 2 cut(s) 105, 851
Eam1104I CTCTTC 3 cut(s) 296, 408, 745
EarI CTCTTC 3 cut(s) 296, 408, 745
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 107, 258
EcoRV GATATC 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 106
FaiI YATR 3 cut(s) 104, 753, 822
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 2 cut(s) 747, 776
FatI CATG 1 cut(s) 102
FauI CCCGC 2 cut(s) 27, 240
Fnu4HI GCNGC 3 cut(s) 12, 150, 186
FokI GGATG 2 cut(s) 446, 720
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 3 cut(s) 12, 150, 186
FspBI CTAG 3 cut(s) 77, 407, 783
GlaI GCGC 4 cut(s) 15, 75, 252, 288
GluI GCNGC 3 cut(s) 12, 150, 186
GsaI CCCAGC 1 cut(s) 644
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 3 cut(s) 17, 77, 290
HaeIII GGCC 3 cut(s) 38, 107, 853
HapII CCGG 3 cut(s) 17, 52, 850
HhaI GCGC 4 cut(s) 16, 76, 253, 289
Hin1I GRCGYC 2 cut(s) 5, 14
Hin1II CATG 1 cut(s) 106
Hin6I GCGC 4 cut(s) 14, 74, 251, 287
HinP1I GCGC 4 cut(s) 14, 74, 251, 287
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 5 cut(s) 392, 520, 779, 806, 825
HpaII CCGG 3 cut(s) 17, 52, 850
HphI GGTGA 2 cut(s) 31, 274
Hpy166II GTNNAC 2 cut(s) 22, 580
Hpy188I TCNGA 5 cut(s) 70, 331, 397, 519, 688
Hpy188III TCNNGA 7 cut(s) 160, 170, 209, 458, 524, 721, 746
Hpy8I GTNNAC 2 cut(s) 22, 580
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 50, 356, 504, 597
HpyCH4III ACNGT 2 cut(s) 759, 841
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 3 cut(s) 49, 665, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 202, 525, 816
HpySE526I ACGT 1 cut(s) 672
Hsp92I GRCGYC 2 cut(s) 5, 14
Hsp92II CATG 1 cut(s) 106
HspAI GCGC 4 cut(s) 14, 74, 251, 287
KasI GGCGCC 1 cut(s) 13
Kzo9I GATC 4 cut(s) 27, 126, 514, 607
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 3 cut(s) 233, 385, 469
Lsp1109I GCAGC 2 cut(s) 136, 197
LweI GCATC 2 cut(s) 36, 689
MaeI CTAG 3 cut(s) 77, 407, 783
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 4 cut(s) 262, 372, 617, 652
MalI GATC 4 cut(s) 29, 128, 516, 609
MbiI CCGCTC 1 cut(s) 34
MboI GATC 4 cut(s) 27, 126, 514, 607
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 2 cut(s) 40, 672
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 2 cut(s) 773, 800
Mox20I TGGCCA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 107
MseI TTAA 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 3 cut(s) 17, 52, 850
MspR9I CCNGG 3 cut(s) 53, 109, 260
MvaI CCWGG 2 cut(s) 109, 260
MwoI GCNNNNNNNGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 1 cut(s) 53
NcoI CCATGG 1 cut(s) 102
NdeII GATC 4 cut(s) 27, 126, 514, 607
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 3 cut(s) 15, 38, 609
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 3 cut(s) 262, 372, 652
PciSI GCTCTTC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 392, 520, 825
PkrI GCNGC 3 cut(s) 13, 151, 187
PleI GAGTC 2 cut(s) 773, 800
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 2 cut(s) 773, 800
Psp6I CCWGG 2 cut(s) 107, 258
PspEI GGTNACC 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 640
PspGI CCWGG 2 cut(s) 107, 258
PspN4I GGNNCC 3 cut(s) 15, 38, 609
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 3 cut(s) 36, 37, 111
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 607
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 3 cut(s) 12, 150, 186
Sau3AI GATC 4 cut(s) 27, 126, 514, 607
Sau96I GGNCC 3 cut(s) 36, 37, 111
SchI GAGTC 2 cut(s) 773, 800
ScrFI CCNGG 3 cut(s) 53, 109, 260
SduI GDGCHC 2 cut(s) 40, 672
SfaNI GCATC 2 cut(s) 36, 689
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 16
SinI GGWCC 1 cut(s) 111
SmlI CTYRAG 2 cut(s) 209, 269
SmoI CTYRAG 2 cut(s) 209, 269
SsiI CCGC 3 cut(s) 11, 34, 247
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 3 cut(s) 77, 407, 783
StyD4I CCNGG 3 cut(s) 51, 107, 258
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 759, 841
TaiI ACGT 1 cut(s) 675
TaqI TCGA 4 cut(s) 30, 294, 312, 623
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 3 cut(s) 392, 520, 825
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 235, 861
TseFI GTSAC 3 cut(s) 262, 372, 652
TseI GCWGC 2 cut(s) 149, 185
Tsp45I GTSAC 3 cut(s) 262, 372, 652
TspDTI ATGAA 5 cut(s) 207, 290, 450, 641, 816
TspGWI ACGGA 1 cut(s) 818
TspRI CASTG 2 cut(s) 235, 861
VpaK11BI GGWCC 1 cut(s) 111
XspI CTAG 3 cut(s) 77, 407, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.