MD10G1204300.v1.1

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010186
Physical Location & Seq
Forward (+)
30338500 .. 30340948
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1204300.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGCTTCGGCGCCGGTGAACAAGATTGAGCGGGCCCACCAGATGTACCGAGAAGGTAGCTACTCGGAGGCGCTAGGGTTTTACACCGAAGCCCTATCCATGGCCAAGACCAAACCCCAGAAGATCGCCCTCCACAGCAACCGAGCTGCTTGCTTCTTGAAGCTCCACGATTTCAAAAAGGCAGCAGATGAATGTACCTCAGTGCTTGAGCTTGATTACAATCACACTGGAGCATTGATGTTGCGGGCGCAGACCTTGGTGACCCTCAAGGAATATCACTCGGCACTCTTTGATGTTCATCGGCTCATCGAGTTAAATCCATCATCAGAAATTTATCAAAACCTTCAAGCCCGATTGAAGACACAAGTGTCACTTGCTCCAATACCTGAATCCGAAGCAGAGCTAGAAGAAGAGGAAGAAGATGAGGAGGATGAAGCAGAGCCAAATAGATATGCGGAGAAAGTGGAGCAATACGAAGAAAAAGAAGATGCTGTATTTTCAAGTGCAGGAAAGGAACAGATTCCTGAGGTTAATGAGACAACTGCTGTTGCCGATGTTATTCCCCCCAAGACACCGGTAAACAGGGAAGTTTCTCCACAAGGAAGGGATCCAAAAGTTAACTACATAAAGACCATTTCCCCAGCTGAAGTTGTTGCCGCTCGTGCAGTCAAGGTATCGTCTGAAAAAGAAAAAGAAAAAGAAAAAGAAAACGGATGGCAAGCAATTCCAAAACCAAAGGGACACTCTTCTTTGGACTATGCACGGTGGGACAGAGTCGAAGATGACTCTAGTGAAGAGGATGATGAGGACGATGATGATGAAGACTCTCAACCTCAGTTTAGATTCCGTGTGAGAACTGTTGGTGTCCGACCAGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

294

Amino Acids

33.22

Weight (kDa)

4.81

Isoelectric Point (pI)

53.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 19 - 96 3.7e-06 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 2 cut(s) 31, 659
AciI CCGC 4 cut(s) 31, 244, 455, 657
AclWI GGATC 2 cut(s) 602, 615
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 330
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 2 cut(s) 47, 196
AfiI CCNNNNNNNGG 1 cut(s) 100
AgeI ACCGGT 1 cut(s) 574
AgsI TTSAA 5 cut(s) 160, 175, 347, 358, 501
AluBI AGCT 6 cut(s) 60, 146, 163, 211, 403, 644
AluI AGCT 6 cut(s) 60, 146, 163, 211, 403, 644
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 2 cut(s) 602, 615
AoxI GGCC 2 cut(s) 33, 102
ApaI GGGCCC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 146, 182
ApoI RAATTY 1 cut(s) 330
AsiGI ACCGGT 1 cut(s) 574
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 3 cut(s) 13, 73, 250
AspS9I GGNCC 2 cut(s) 33, 34
AsuHPI GGTGA 2 cut(s) 28, 271
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 37
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 365, 828
BbvI GCAGC 2 cut(s) 133, 194
BccI CCATC 2 cut(s) 328, 708
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 3 cut(s) 74, 404, 789
BfoI RGCGCY 2 cut(s) 14, 74
BisI GCNGC 3 cut(s) 147, 183, 657
BlsI GCNGC 3 cut(s) 148, 184, 658
BmgT120I GGNCC 2 cut(s) 33, 34
BmiI GGNNCC 3 cut(s) 12, 35, 609
BmsI GCATC 1 cut(s) 478
BpiI GAAGAC 2 cut(s) 365, 828
BpmI CTGGAG 1 cut(s) 249
BpuEI CTTGAG 2 cut(s) 227, 251
BsaBI GATNNNNATC 2 cut(s) 219, 297
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 99, 255
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse118I RCCGGY 2 cut(s) 13, 574
Bse1I ACTGG 2 cut(s) 232, 872
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 2 cut(s) 219, 297
BseDI CCNNGG 2 cut(s) 99, 255
BseGI GGATG 3 cut(s) 436, 719, 805
BseJI GATNNNNATC 2 cut(s) 219, 297
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 213, 516, 848
BseNI ACTGG 2 cut(s) 232, 872
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 133, 194
BseYI CCCAGC 1 cut(s) 640
BsgI GTGCAG 2 cut(s) 525, 684
BshFI GGCC 2 cut(s) 35, 104
BshNI GGYRCC 1 cut(s) 10
BshTI ACCGGT 1 cut(s) 574
BsiSI CCGG 2 cut(s) 14, 575
BslFI GGGAC 2 cut(s) 753, 782
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 530
BsmFI GGGAC 2 cut(s) 753, 782
BsnI GGCC 2 cut(s) 35, 104
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 2 cut(s) 123, 607
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 4 cut(s) 31, 244, 455, 657
BspANI GGCC 2 cut(s) 35, 104
BspCNI CTCAG 3 cut(s) 212, 517, 847
BspLI GGNNCC 3 cut(s) 12, 35, 609
BspPI GGATC 2 cut(s) 602, 615
BspT107I GGYRCC 1 cut(s) 10
BsrBI CCGCTC 2 cut(s) 31, 659
BsrFI RCCGGY 2 cut(s) 13, 574
BsrI ACTGG 2 cut(s) 232, 872
BssAI RCCGGY 2 cut(s) 13, 574
BssECI CCNNGG 2 cut(s) 99, 255
BssMI GATC 2 cut(s) 123, 607
BssNI GRCGYC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 99, 255
Bst2BI CACGAG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 765, 859
Bst6I CTCTTC 3 cut(s) 405, 751, 789
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 33, 151, 246, 720
BstDEI CTNAG 3 cut(s) 199, 525, 834
BstDSI CCRYGG 1 cut(s) 99
BstEII GGTNACC 1 cut(s) 259
BstF5I GGATG 3 cut(s) 436, 719, 805
BstH2I RGCGCY 2 cut(s) 14, 74
BstHHI GCGC 3 cut(s) 13, 73, 250
BstKTI GATC 2 cut(s) 126, 610
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 2 cut(s) 123, 607
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstPI GGTNACC 1 cut(s) 259
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 133, 194
BstV2I GAAGAC 2 cut(s) 365, 828
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 2 cut(s) 35, 104
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 3 cut(s) 436, 719, 805
BtsIMutI CAGTG 3 cut(s) 207, 225, 879
Cac8I GCNNGC 4 cut(s) 33, 151, 246, 720
CfoI GCGC 3 cut(s) 13, 73, 250
Cfr10I RCCGGY 2 cut(s) 13, 574
Cfr13I GGNCC 2 cut(s) 33, 34
Csp6I GTAC 2 cut(s) 46, 195
CspAI ACCGGT 1 cut(s) 574
CviAII CATG 1 cut(s) 100
CviQI GTAC 2 cut(s) 46, 195
DdeI CTNAG 3 cut(s) 199, 525, 834
DinI GGCGCC 1 cut(s) 12
DpnI GATC 2 cut(s) 125, 609
DpnII GATC 2 cut(s) 123, 607
DrdI GACNNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 3 cut(s) 405, 751, 789
EarI CTCTTC 3 cut(s) 405, 751, 789
Eco130I CCWWGG 2 cut(s) 99, 255
Eco24I GRGCYC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 259
EcoO65I GGTNACC 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 99, 255
EcoT38I GRGCYC 1 cut(s) 37
EgeI GGCGCC 1 cut(s) 12
EheI GGCGCC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 99, 255
FaeI CATG 1 cut(s) 103
FaiI YATR 4 cut(s) 101, 453, 626, 759
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 753, 782
FatI CATG 1 cut(s) 99
FauI CCCGC 2 cut(s) 24, 237
Fnu4HI GCNGC 3 cut(s) 147, 183, 657
FokI GGATG 3 cut(s) 443, 726, 812
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 3 cut(s) 147, 183, 657
FspBI CTAG 3 cut(s) 74, 404, 789
GlaI GCGC 3 cut(s) 12, 72, 249
GluI GCNGC 3 cut(s) 147, 183, 657
GsaI CCCAGC 1 cut(s) 644
GsuI CTGGAG 1 cut(s) 249
HaeII RGCGCY 2 cut(s) 14, 74
HaeIII GGCC 2 cut(s) 35, 104
HapII CCGG 2 cut(s) 14, 575
HhaI GCGC 3 cut(s) 13, 73, 250
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 3 cut(s) 11, 71, 248
HinP1I GCGC 3 cut(s) 11, 71, 248
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 6 cut(s) 389, 520, 774, 785, 824, 843
HpaI GTTAAC 1 cut(s) 619
HpaII CCGG 2 cut(s) 14, 575
HphI GGTGA 2 cut(s) 28, 271
Hpy166II GTNNAC 3 cut(s) 19, 580, 619
Hpy188I TCNGA 5 cut(s) 67, 328, 394, 682, 869
Hpy188III TCNNGA 2 cut(s) 157, 524
Hpy8I GTNNAC 3 cut(s) 19, 580, 619
HpyAV CCTTC 3 cut(s) 47, 353, 597
HpyCH4III ACNGT 2 cut(s) 765, 859
HpyCH4V TGCA 3 cut(s) 506, 665, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 3 cut(s) 199, 525, 834
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 3 cut(s) 11, 71, 248
KasI GGCGCC 1 cut(s) 10
KspAI GTTAAC 1 cut(s) 619
Kzo9I GATC 2 cut(s) 123, 607
LmnI GCTCC 4 cut(s) 168, 230, 382, 466
Lsp1109I GCAGC 2 cut(s) 133, 194
LweI GCATC 1 cut(s) 478
MaeI CTAG 3 cut(s) 74, 404, 789
MaeIII GTNAC 2 cut(s) 259, 369
MalI GATC 2 cut(s) 125, 609
MbiI CCGCTC 2 cut(s) 31, 659
MboI GATC 2 cut(s) 123, 607
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 2 cut(s) 330, 723
MluNI TGGCCA 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 11
MlyI GAGTC 3 cut(s) 779, 783, 818
Mox20I TGGCCA 1 cut(s) 104
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 104
MseI TTAA 3 cut(s) 314, 531, 618
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 2 cut(s) 14, 575
MwoI GCNNNNNNNGC 1 cut(s) 662
NarI GGCGCC 1 cut(s) 11
NcoI CCATGG 1 cut(s) 99
NdeII GATC 2 cut(s) 123, 607
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 3 cut(s) 12, 35, 609
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 259, 369
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 389, 520, 843
PflFI GACNNNGTC 1 cut(s) 773
PinAI ACCGGT 1 cut(s) 574
PkrI GCNGC 3 cut(s) 148, 184, 658
PleI GAGTC 3 cut(s) 779, 782, 818
PluTI GGCGCC 1 cut(s) 14
PpsI GAGTC 3 cut(s) 779, 782, 818
PspEI GGTNACC 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 640
PspN4I GGNNCC 3 cut(s) 12, 35, 609
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 2 cut(s) 33, 34
PsuI RGATCY 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 773
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 47, 196
RsaNI GTAC 2 cut(s) 46, 195
SaqAI TTAA 3 cut(s) 314, 531, 618
SatI GCNGC 3 cut(s) 147, 183, 657
Sau3AI GATC 2 cut(s) 123, 607
Sau96I GGNCC 2 cut(s) 33, 34
SchI GAGTC 3 cut(s) 779, 783, 818
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 1 cut(s) 478
SfoI GGCGCC 1 cut(s) 12
SgrAI CRCCGGYG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 206, 266
SmoI CTYRAG 2 cut(s) 206, 266
Sse9I AATT 2 cut(s) 330, 723
SsiI CCGC 4 cut(s) 31, 244, 455, 657
SspDI GGCGCC 1 cut(s) 10
SspMI CTAG 3 cut(s) 74, 404, 789
StyI CCWWGG 2 cut(s) 99, 255
TaaI ACNGT 2 cut(s) 765, 859
TaqI TCGA 2 cut(s) 309, 777
TasI AATT 2 cut(s) 330, 723
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 3 cut(s) 389, 520, 843
Tru1I TTAA 3 cut(s) 314, 531, 618
Tru9I TTAA 3 cut(s) 314, 531, 618
TscAI CASTG 3 cut(s) 207, 232, 879
TseFI GTSAC 2 cut(s) 259, 369
TseI GCWGC 2 cut(s) 146, 182
Tsp45I GTSAC 2 cut(s) 259, 369
TspDTI ATGAA 4 cut(s) 204, 287, 447, 834
TspGWI ACGGA 2 cut(s) 726, 836
TspRI CASTG 3 cut(s) 207, 232, 879
Tth111I GACNNNGTC 1 cut(s) 773
XapI RAATTY 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 3 cut(s) 74, 404, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.