Prupe.4G137600_v2.0.a1

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010186
Physical Location & Seq
Reverse (-)
7646093 .. 7649364
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G137600.1

Sequence Viewer

Length: 867 bp
ATGGCGTCGGCGCCGGTGAACAAGATCGAGCGGGCCCACCTGATGTACCGGGAAGGGAGGTACTCGGAGGCCCTAGGGTTTTACACGGAAGCCCTATCCATGGCCAAGACGAAGCCCCAGAAGATCGCTTTGCACAGCAACAGAGCTGCTTGCTTCTTGAAGCTTCACGATTTCAAAAAGGCTGCAGATGAATGCACCTCAGTGCTTGAGCTTGATTTCAATCACACTGGAGCTTTGATGTTGCGGGCGCAGACCCTAGTGACCCTCAAGGAATATCACTCAGCTCTCTTTGATGTTCATCGGCTGATAGAGTTAAATCCATCATCAGAAGTGTATCAAAACCTTCAAGCCCGTTTAAAGACACAAGTGTCCCTTGCTCCAATACCTGAATCCGACGCAGAGCTAGAAGAAGAGGAAGAAGACGAGGATGAAGTAGAGCTAAATGGATATGGGAAGGAAGAGGAGAAATACGAAGGACAAGAAGGTGCAGTATTTTCAGGTGCAGTAAAGGATCAGTTTGCTGAGGTTAATAGGACGACTGCTGTTGCTGATGTTATTCCCCCCAAGACACCAGTAAACAAGGAAGTTCCTGAACAAGGAAGGGATCCAAAAGTTAATTACACAAAGACTACTTCCCCAGCTGAAGTTGCTGCCCCTCGTGCAGAACTCATCAAAGTACCGTCTGAAAAAGAAAAAGGATGGCAAGCAATCCCAAAACCGAAGGGGCACTCTTCTCTGGACTATGCACGGTGGGACACAGTAGAAGATGACTCTAGTGAAGATGACGACGACGACGATGATGATGAAGAGTCTCAACCTCAGTATAGATTCCGTGTAAGAACTGTTGGTGTCCGACCAGTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

289

Amino Acids

32.41

Weight (kDa)

4.83

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 1 cut(s) 31
AciI CCGC 2 cut(s) 31, 244
AclWI GGATC 3 cut(s) 519, 599, 612
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 663
AcyI GRCGYC 2 cut(s) 5, 11
AfaI GTAC 3 cut(s) 47, 62, 678
AfiI CCNNNNNNNGG 2 cut(s) 100, 596
AgsI TTSAA 4 cut(s) 160, 175, 220, 347
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 8 cut(s) 146, 163, 211, 233, 284, 403, 439, 641
AluI AGCT 8 cut(s) 146, 163, 211, 233, 284, 403, 439, 641
Alw26I GTCTC 1 cut(s) 816
AlwI GGATC 3 cut(s) 519, 599, 612
AoxI GGCC 3 cut(s) 33, 69, 102
ApaI GGGCCC 1 cut(s) 37
ApeKI GCWGC 3 cut(s) 146, 182, 650
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 2 cut(s) 13, 250
AspS9I GGNCC 3 cut(s) 33, 34, 70
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 28
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 2 cut(s) 37, 729
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 37
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 426
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 133, 169, 637
BccI CCATC 2 cut(s) 328, 693
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 816
BfaI CTAG 4 cut(s) 74, 257, 404, 774
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 14
BisI GCNGC 3 cut(s) 147, 183, 651
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 3 cut(s) 148, 184, 652
Bme1390I CCNGG 1 cut(s) 50
BmgT120I GGNCC 3 cut(s) 33, 34, 70
BmiI GGNNCC 3 cut(s) 12, 35, 606
BmrFI CCNGG 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 227, 251
BpuMI CCSGG 1 cut(s) 50
BsaBI GATNNNNATC 2 cut(s) 219, 297
BsaHI GRCGYC 2 cut(s) 5, 11
BsaJI CCNNGG 2 cut(s) 73, 99
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 596
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 232, 572, 857
Bse8I GATNNNNATC 2 cut(s) 219, 297
BseDI CCNNGG 2 cut(s) 73, 99
BseGI GGATG 2 cut(s) 433, 704
BseJI GATNNNNATC 2 cut(s) 219, 297
BseLI CCNNNNNNNGG 2 cut(s) 100, 596
BseMII CTCAG 4 cut(s) 213, 294, 513, 833
BseNI ACTGG 3 cut(s) 232, 572, 857
BseRI GAGGAG 1 cut(s) 476
BseSI GKGCMC 2 cut(s) 37, 729
BseXI GCAGC 3 cut(s) 133, 169, 637
BseYI CCCAGC 1 cut(s) 637
BsgI GTGCAG 3 cut(s) 507, 522, 681
BshFI GGCC 3 cut(s) 35, 71, 104
BshNI GGYRCC 1 cut(s) 10
BsiSI CCGG 2 cut(s) 14, 49
BslFI GGGAC 2 cut(s) 355, 767
BslI CCNNNNNNNGG 2 cut(s) 100, 596
BsmAI GTCTC 1 cut(s) 816
BsmFI GGGAC 2 cut(s) 355, 767
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 3 cut(s) 35, 71, 104
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 37, 729
Bsp143I GATC 4 cut(s) 24, 123, 511, 604
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 2 cut(s) 31, 244
BspANI GGCC 3 cut(s) 35, 71, 104
BspCNI CTCAG 4 cut(s) 212, 293, 514, 832
BspLI GGNNCC 3 cut(s) 12, 35, 606
BspMAI CTGCAG 1 cut(s) 187
BspPI GGATC 3 cut(s) 519, 599, 612
BspT107I GGYRCC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 232, 572, 857
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 73, 99
BssMI GATC 4 cut(s) 24, 123, 511, 604
BssNI GRCGYC 2 cut(s) 5, 11
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 2 cut(s) 73, 99
Bst2BI CACGAG 1 cut(s) 657
Bst4CI ACNGT 4 cut(s) 681, 750, 760, 844
Bst6I CTCTTC 4 cut(s) 405, 453, 736, 801
BstACI GRCGYC 2 cut(s) 5, 11
BstC8I GCNNGC 4 cut(s) 33, 151, 246, 705
BstDEI CTNAG 4 cut(s) 199, 280, 522, 819
BstDSI CCRYGG 1 cut(s) 99
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 2 cut(s) 433, 704
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 2 cut(s) 13, 250
BstKTI GATC 4 cut(s) 27, 126, 514, 607
BstMAI GTCTC 1 cut(s) 816
BstMBI GATC 4 cut(s) 24, 123, 511, 604
BstMWI GCNNNNNNNGC 2 cut(s) 647, 659
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 2 cut(s) 37, 729
BstV1I GCAGC 3 cut(s) 133, 169, 637
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 3 cut(s) 35, 71, 104
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 2 cut(s) 433, 704
BtsIMutI CAGTG 2 cut(s) 207, 225
Cac8I GCNNGC 4 cut(s) 33, 151, 246, 705
CfoI GCGC 2 cut(s) 13, 250
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 3 cut(s) 33, 34, 70
CseI GACGC 1 cut(s) 404
Csp6I GTAC 3 cut(s) 46, 61, 677
CviAII CATG 1 cut(s) 100
CviQI GTAC 3 cut(s) 46, 61, 677
DdeI CTNAG 4 cut(s) 199, 280, 522, 819
DinI GGCGCC 1 cut(s) 12
DpnI GATC 4 cut(s) 26, 125, 513, 606
DpnII GATC 4 cut(s) 24, 123, 511, 604
DraI TTTAAA 1 cut(s) 357
DrdI GACNNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 4 cut(s) 405, 453, 736, 801
EarI CTCTTC 4 cut(s) 405, 453, 736, 801
Eco130I CCWWGG 2 cut(s) 73, 99
Eco24I GRGCYC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 663
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 73, 99
EcoT38I GRGCYC 1 cut(s) 37
EgeI GGCGCC 1 cut(s) 12
EheI GGCGCC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 73, 99
FaeI CATG 1 cut(s) 103
FaiI YATR 4 cut(s) 101, 450, 744, 825
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 355, 767
FatI CATG 1 cut(s) 99
FauI CCCGC 2 cut(s) 24, 237
Fnu4HI GCNGC 3 cut(s) 147, 183, 651
FokI GGATG 2 cut(s) 440, 711
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 3 cut(s) 147, 183, 651
FspBI CTAG 4 cut(s) 74, 257, 404, 774
GlaI GCGC 2 cut(s) 12, 249
GluI GCNGC 3 cut(s) 147, 183, 651
GsaI CCCAGC 1 cut(s) 641
GsuI CTGGAG 1 cut(s) 249
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 3 cut(s) 35, 71, 104
HapII CCGG 2 cut(s) 14, 49
HgaI GACGC 1 cut(s) 404
HhaI GCGC 2 cut(s) 13, 250
Hin1I GRCGYC 2 cut(s) 5, 11
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 2 cut(s) 11, 248
HinP1I GCGC 2 cut(s) 11, 248
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 4 cut(s) 389, 770, 809, 828
HpaII CCGG 2 cut(s) 14, 49
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 19, 577
Hpy188I TCNGA 5 cut(s) 67, 328, 394, 685, 854
Hpy188III TCNNGA 4 cut(s) 157, 167, 590, 737
Hpy8I GTNNAC 2 cut(s) 19, 577
Hpy99I CGWCG 5 cut(s) 10, 398, 791, 794, 797
HpyAV CCTTC 7 cut(s) 47, 353, 448, 467, 476, 594, 715
HpyCH4III ACNGT 4 cut(s) 681, 750, 760, 844
HpyCH4V TGCA 7 cut(s) 133, 185, 195, 488, 503, 662, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 647, 659
HpyF3I CTNAG 4 cut(s) 199, 280, 522, 819
Hsp92I GRCGYC 2 cut(s) 5, 11
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 2 cut(s) 11, 248
KasI GGCGCC 1 cut(s) 10
Kzo9I GATC 4 cut(s) 24, 123, 511, 604
LmnI GCTCC 2 cut(s) 230, 382
Lsp1109I GCAGC 3 cut(s) 133, 169, 637
MaeI CTAG 4 cut(s) 74, 257, 404, 774
MaeIII GTNAC 1 cut(s) 259
MalI GATC 4 cut(s) 26, 125, 513, 606
MbiI CCGCTC 1 cut(s) 31
MboI GATC 4 cut(s) 24, 123, 511, 604
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 37, 729
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 1 cut(s) 616
MluNI TGGCCA 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 11
MlyI GAGTC 2 cut(s) 764, 818
MmeI TCCRAC 1 cut(s) 417
Mox20I TGGCCA 1 cut(s) 104
MscI TGGCCA 1 cut(s) 104
MseI TTAA 5 cut(s) 314, 356, 528, 615, 861
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 2 cut(s) 14, 49
MspR9I CCNGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 647, 659
NarI GGCGCC 1 cut(s) 11
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 99
NdeII GATC 4 cut(s) 24, 123, 511, 604
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 3 cut(s) 12, 35, 606
NmuCI GTSAC 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 792
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 2 cut(s) 389, 828
PkrI GCNGC 3 cut(s) 148, 184, 652
PleI GAGTC 2 cut(s) 764, 817
PluTI GGCGCC 1 cut(s) 14
PpsI GAGTC 2 cut(s) 764, 817
PspFI CCCAGC 1 cut(s) 637
PspN4I GGNNCC 3 cut(s) 12, 35, 606
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 3 cut(s) 33, 34, 70
PstI CTGCAG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 3 cut(s) 47, 62, 678
RsaNI GTAC 3 cut(s) 46, 61, 677
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 5 cut(s) 314, 356, 528, 615, 861
SatI GCNGC 3 cut(s) 147, 183, 651
Sau3AI GATC 4 cut(s) 24, 123, 511, 604
Sau96I GGNCC 3 cut(s) 33, 34, 70
SchI GAGTC 2 cut(s) 764, 818
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 2 cut(s) 37, 729
SfcI CTRYAG 1 cut(s) 183
SfoI GGCGCC 1 cut(s) 12
SgrAI CRCCGGYG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 206, 266
SmoI CTYRAG 2 cut(s) 206, 266
Sse9I AATT 1 cut(s) 616
SsiI CCGC 2 cut(s) 31, 244
SspDI GGCGCC 1 cut(s) 10
SspMI CTAG 4 cut(s) 74, 257, 404, 774
StyD4I CCNGG 1 cut(s) 48
StyI CCWWGG 2 cut(s) 73, 99
TaaI ACNGT 4 cut(s) 681, 750, 760, 844
TaqI TCGA 1 cut(s) 27
TasI AATT 1 cut(s) 616
TfiI GAWTC 2 cut(s) 389, 828
Tru1I TTAA 5 cut(s) 314, 356, 528, 615, 861
Tru9I TTAA 5 cut(s) 314, 356, 528, 615, 861
TscAI CASTG 2 cut(s) 207, 232
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 146, 182, 650
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 4 cut(s) 204, 287, 444, 819
TspGWI ACGGA 2 cut(s) 101, 821
TspRI CASTG 2 cut(s) 207, 232
XagI CCTNNNNNAGG 1 cut(s) 594
XmaJI CCTAGG 1 cut(s) 73
XspI CTAG 4 cut(s) 74, 257, 404, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.