pycom10g17500

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010186
Physical Location & Seq
Forward (+)
20736576 .. 20737377
802 bp
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UTR
Exon/CDS
Intron
pycom10g17500.1

Sequence Viewer

Length: 378 bp
ATGATGGCGTCGGCGCCGGTGAACAAGATTGAGCGGGCCCACCAGATGTACCGGGAAGGTAGCTACTCGGAGGCGCTAGGGTTTTACACCGAAGCCCTATCCATGGCCAAGACCAAACCCCAGAAGATCGCCCTCCACAGCAACCGAGCTGCTTGCTTCTTGAAGCTCCGCGATTTCAAAAAGGCAGCAGATGAATGTACCTCAGTGCTTGAGCTTGATTACAATCACACTGGAGCATTGATGTTGCGGGCGCAGACCTTGGTGACCCTCAAGGAATATCACTCGGCACTCTTTGATGTTCATCGGCTCATTGAGTTAAATCCATCATCAGAAGTTTATCAAAACCTTCAAGCCCGATTGAAGACACAAGTGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

126

Amino Acids

14.21

Weight (kDa)

8.91

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 20 - 97 3.6e-07 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 34
AccII CGCG 1 cut(s) 171
AciI CCGC 3 cut(s) 34, 169, 247
AcoI YGGCCR 1 cut(s) 105
AcyI GRCGYC 2 cut(s) 8, 14
AfaI GTAC 2 cut(s) 50, 199
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 4 cut(s) 163, 178, 350, 361
AluBI AGCT 4 cut(s) 63, 149, 166, 214
AluI AGCT 4 cut(s) 63, 149, 166, 214
AoxI GGCC 2 cut(s) 36, 105
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 149, 185
AspLEI GCGC 3 cut(s) 16, 76, 253
AspS9I GGNCC 2 cut(s) 36, 37
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 31, 274
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 136, 197
BccI CCATC 1 cut(s) 331
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BcnI CCSGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 2 cut(s) 17, 77
BisI GCNGC 2 cut(s) 150, 186
BlsI GCNGC 2 cut(s) 151, 187
Bme1390I CCNGG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 36, 37
BmiI GGNNCC 2 cut(s) 15, 38
BmrFI CCNGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 230, 254
BpuMI CCSGG 1 cut(s) 53
BsaBI GATNNNNATC 2 cut(s) 222, 300
BsaHI GRCGYC 2 cut(s) 8, 14
BsaJI CCNNGG 2 cut(s) 102, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 235
Bse8I GATNNNNATC 2 cut(s) 222, 300
BseDI CCNNGG 2 cut(s) 102, 258
BseJI GATNNNNATC 2 cut(s) 222, 300
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 235
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 2 cut(s) 136, 197
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 38, 107
BshNI GGYRCC 1 cut(s) 13
BsiSI CCGG 2 cut(s) 17, 52
BslI CCNNNNNNNGG 1 cut(s) 103
BsnI GGCC 2 cut(s) 38, 107
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 3 cut(s) 34, 169, 247
BspANI GGCC 2 cut(s) 38, 107
BspCNI CTCAG 1 cut(s) 215
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 15, 38
BspT107I GGYRCC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 102, 258
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 2 cut(s) 8, 14
BssT1I CCWWGG 2 cut(s) 102, 258
BstACI GRCGYC 2 cut(s) 8, 14
BstC8I GCNNGC 3 cut(s) 36, 154, 249
BstDEI CTNAG 1 cut(s) 202
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 262
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 2 cut(s) 17, 77
BstHHI GCGC 3 cut(s) 16, 76, 253
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 51
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 136, 197
BstV2I GAAGAC 1 cut(s) 368
BsuRI GGCC 2 cut(s) 38, 107
BtgI CCRYGG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 210, 228
Cac8I GCNNGC 3 cut(s) 36, 154, 249
CfoI GCGC 3 cut(s) 16, 76, 253
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 36, 37
Csp6I GTAC 2 cut(s) 49, 198
CviAII CATG 1 cut(s) 103
CviJI RGCY 9 cut(s) 38, 63, 95, 107, 149, 166, 214, 307, 353
CviKI_1 RGCY 9 cut(s) 38, 63, 95, 107, 149, 166, 214, 307, 353
CviQI GTAC 2 cut(s) 49, 198
DdeI CTNAG 1 cut(s) 202
DinI GGCGCC 1 cut(s) 15
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 102, 258
Eco24I GRGCYC 1 cut(s) 40
Eco91I GGTNACC 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 102, 258
EcoT38I GRGCYC 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 102, 258
FaeI CATG 1 cut(s) 106
FaiI YATR 2 cut(s) 104, 376
FatI CATG 1 cut(s) 102
FauI CCCGC 2 cut(s) 27, 240
Fnu4HI GCNGC 2 cut(s) 150, 186
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 150, 186
FspBI CTAG 1 cut(s) 77
GlaI GCGC 3 cut(s) 15, 75, 252
GluI GCNGC 2 cut(s) 150, 186
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 2 cut(s) 17, 77
HaeIII GGCC 2 cut(s) 38, 107
HapII CCGG 2 cut(s) 17, 52
HhaI GCGC 3 cut(s) 16, 76, 253
Hin1I GRCGYC 2 cut(s) 8, 14
Hin1II CATG 1 cut(s) 106
Hin6I GCGC 3 cut(s) 14, 74, 251
HinP1I GCGC 3 cut(s) 14, 74, 251
HpaII CCGG 2 cut(s) 17, 52
HphI GGTGA 2 cut(s) 31, 274
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 2 cut(s) 70, 331
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 50, 356
HpyF3I CTNAG 1 cut(s) 202
Hsp92I GRCGYC 2 cut(s) 8, 14
Hsp92II CATG 1 cut(s) 106
HspAI GCGC 3 cut(s) 14, 74, 251
KasI GGCGCC 1 cut(s) 13
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 2 cut(s) 171, 233
LpnPI CCDG 5 cut(s) 30, 56, 65, 134, 216
Lsp1109I GCAGC 2 cut(s) 136, 197
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 1 cut(s) 262
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 34
MboI GATC 1 cut(s) 126
MboII GAAGA 2 cut(s) 136, 373
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 14
MnlI CCTC 4 cut(s) 64, 143, 211, 278
Mox20I TGGCCA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 107
MseI TTAA 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 2 cut(s) 17, 52
MspR9I CCNGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 171
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 1 cut(s) 53
NcoI CCATGG 1 cut(s) 102
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 2 cut(s) 15, 38
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 262
PkrI GCNGC 2 cut(s) 151, 187
PluTI GGCGCC 1 cut(s) 17
PspEI GGTNACC 1 cut(s) 262
PspN4I GGNNCC 2 cut(s) 15, 38
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 2 cut(s) 36, 37
RsaI GTAC 2 cut(s) 50, 199
RsaNI GTAC 2 cut(s) 49, 198
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 2 cut(s) 150, 186
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 2 cut(s) 36, 37
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 40
SetI ASST 8 cut(s) 61, 65, 151, 168, 203, 216, 260, 348
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 209, 269
SmoI CTYRAG 2 cut(s) 209, 269
SsiI CCGC 3 cut(s) 34, 169, 247
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 2 cut(s) 102, 258
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 210, 235
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 2 cut(s) 149, 185
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 207, 290
TspRI CASTG 2 cut(s) 210, 235
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.