RLG00000032904

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010186
Physical Location & Seq
Reverse (-)
20639941 .. 20642751
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032904

Sequence Viewer

Length: 843 bp
ATGGCATCGGCGGCGCCGGCGAACAAGATCGAGCGGGCCCACCTCATGTACCGGGAAGGGAGGTACCAGGAGGCCCTGGGATTCTACACCGAAGCCCTATCCATGGCCAAGACCAAACCCCAGAAGATTGCCCTCCACAGCAATAGAGCCGCTTGCTTCCTCAAGCTTCACGATTTCAAAAAGGCTGCAGAGGAATGTACCTCAGTGCTTGAGCTTGATTACAATCACACTGGAGCATTGATGTTGCGGGCTCAGACCCTGGTGACACTAAAGGAGTACCGCTCAGCACTCTTTGATGTTAATAGGCTCATAGAGTTAAATCCATCATCCGAAGTGTATCAGAACCTTGAAGCCCGTTTAAGGACGCAAGTGTCACTGGCTCCAATACCTGAGTCCGAAGCAGAGCTAGAAGAAGAGGATGAAGCAGAGCCAAATAGATATGAGGAGAGACAAGAGCAATATATAGGAAAAGAAGATGTCCTACCTTTGAGGGAAGGAAGGGATCAGCTTGCTGAGGTTAATAGGACTACTATTGCTGATGTCATCCCACTCAAGACACCAATGAACAAGGACTCTGCTGAAGAAGTAAGGGATCCAAAAGAAAACTCTATTAAGACTACTTCCCCGGCTGAAGTTACACCAGATAACATCAAAGTATCACCTGAAAAAGAAAAGGGATGGCAAGCAATCCCAAAACCGAAGGGACACTCTTCTCTGGACTATGGACGGTGGGACAGTGTGGAAGATGACTCTAGCGAAGAAGATGATGATGATGATGAGGAAGAGTCTCGACCACAGTATCGGTTCCGTGTAAAAACTGTTGGTGTTCGGCCGGTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

281

Amino Acids

31.94

Weight (kDa)

4.93

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 20 - 97 2.7e-06 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 13, 63
AccBSI CCGCTC 2 cut(s) 34, 282
AciI CCGC 5 cut(s) 11, 34, 150, 247, 280
AclWI GGATC 3 cut(s) 510, 587, 600
AcoI YGGCCR 2 cut(s) 105, 830
AcuI CTGAAG 2 cut(s) 600, 651
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 4 cut(s) 50, 65, 199, 278
AfiI CCNNNNNNNGG 2 cut(s) 103, 360
AgsI TTSAA 2 cut(s) 178, 350
AjnI CCWGG 3 cut(s) 66, 75, 258
AluBI AGCT 4 cut(s) 166, 214, 406, 508
AluI AGCT 4 cut(s) 166, 214, 406, 508
Alw26I GTCTC 2 cut(s) 442, 792
AlwI GGATC 3 cut(s) 510, 587, 600
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 4 cut(s) 36, 72, 105, 830
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 3 cut(s) 36, 37, 73
AsuC2I CCSGG 2 cut(s) 53, 626
AsuHPI GGTGA 2 cut(s) 274, 651
BaeGI GKGCMC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 782, 815
BalI TGGCCA 1 cut(s) 107
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 2 cut(s) 13, 63
BanII GRGCYC 2 cut(s) 40, 253
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 331, 672
BciT130I CCWGG 3 cut(s) 68, 77, 260
BcnI CCSGG 2 cut(s) 53, 626
BcoDI GTCTC 2 cut(s) 442, 792
BfaI CTAG 2 cut(s) 407, 753
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 17
BisI GCNGC 3 cut(s) 12, 150, 186
BlpI GCTNAGC 1 cut(s) 283
BlsI GCNGC 3 cut(s) 13, 151, 187
Bme1390I CCNGG 5 cut(s) 53, 68, 77, 260, 626
BmgT120I GGNCC 3 cut(s) 36, 37, 73
BmiI GGNNCC 6 cut(s) 15, 38, 65, 381, 594, 806
BmrFI CCNGG 5 cut(s) 53, 68, 77, 260, 626
BmsI GCATC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 283
BpuEI CTTGAG 3 cut(s) 146, 230, 536
BpuMI CCSGG 2 cut(s) 53, 626
BsaBI GATNNNNATC 1 cut(s) 222
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 5 cut(s) 75, 76, 102, 258, 624
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 360
Bse118I RCCGGY 2 cut(s) 16, 832
Bse1I ACTGG 2 cut(s) 235, 381
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 3 cut(s) 68, 77, 260
BseDI CCNNGG 5 cut(s) 75, 76, 102, 258, 624
BseGI GGATG 4 cut(s) 326, 424, 543, 683
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 103, 360
BseMII CTCAG 5 cut(s) 216, 266, 297, 381, 504
BseNI ACTGG 2 cut(s) 235, 381
BseRI GAGGAG 1 cut(s) 458
BseSI GKGCMC 1 cut(s) 40
BseX3I CGGCCG 1 cut(s) 830
BseXI GCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 833
BshFI GGCC 4 cut(s) 38, 74, 107, 832
BshNI GGYRCC 2 cut(s) 13, 63
BsiEI CGRYCG 1 cut(s) 833
BsiSI CCGG 4 cut(s) 17, 52, 626, 833
BslFI GGGAC 2 cut(s) 717, 746
BslI CCNNNNNNNGG 2 cut(s) 103, 360
BsmAI GTCTC 2 cut(s) 442, 792
BsmFI GGGAC 2 cut(s) 717, 746
BsnI GGCC 4 cut(s) 38, 74, 107, 832
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 40, 253
Bsp143I GATC 3 cut(s) 27, 502, 592
Bsp1720I GCTNAGC 1 cut(s) 283
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 5 cut(s) 11, 34, 150, 247, 280
BspANI GGCC 4 cut(s) 38, 74, 107, 832
BspCNI CTCAG 5 cut(s) 215, 265, 296, 382, 505
BspLI GGNNCC 6 cut(s) 15, 38, 65, 381, 594, 806
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 3 cut(s) 510, 587, 600
BspT107I GGYRCC 2 cut(s) 13, 63
BsrBI CCGCTC 2 cut(s) 34, 282
BsrFI RCCGGY 2 cut(s) 16, 832
BsrI ACTGG 2 cut(s) 235, 381
BssAI RCCGGY 2 cut(s) 16, 832
BssECI CCNNGG 5 cut(s) 75, 76, 102, 258, 624
BssMI GATC 3 cut(s) 27, 502, 592
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 68, 77, 260
Bst4CI ACNGT 4 cut(s) 729, 737, 798, 820
Bst6I CTCTTC 3 cut(s) 408, 715, 777
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 6 cut(s) 18, 36, 154, 249, 510, 684
BstDEI CTNAG 5 cut(s) 202, 252, 283, 390, 513
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 4 cut(s) 326, 424, 543, 683
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 3 cut(s) 30, 505, 595
BstMAI GTCTC 2 cut(s) 442, 792
BstMBI GATC 3 cut(s) 27, 502, 592
BstMCI CGRYCG 1 cut(s) 833
BstMWI GCNNNNNNNGC 2 cut(s) 11, 17
BstNI CCWGG 3 cut(s) 68, 77, 260
BstSCI CCNGG 5 cut(s) 51, 66, 75, 258, 624
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BstZI CGGCCG 1 cut(s) 830
BsuRI GGCC 4 cut(s) 38, 74, 107, 832
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 4 cut(s) 326, 424, 543, 683
BtsIMutI CAGTG 4 cut(s) 210, 228, 374, 742
Cac8I GCNNGC 6 cut(s) 18, 36, 154, 249, 510, 684
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 2 cut(s) 16, 832
Cfr13I GGNCC 3 cut(s) 36, 37, 73
CseI GACGC 1 cut(s) 373
Csp6I GTAC 4 cut(s) 49, 64, 198, 277
CviAII CATG 2 cut(s) 46, 103
CviQI GTAC 4 cut(s) 49, 64, 198, 277
DdeI CTNAG 5 cut(s) 202, 252, 283, 390, 513
DinI GGCGCC 1 cut(s) 15
DpnI GATC 3 cut(s) 29, 504, 594
DpnII GATC 3 cut(s) 27, 502, 592
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 2 cut(s) 105, 830
EagI CGGCCG 1 cut(s) 830
Eam1104I CTCTTC 3 cut(s) 408, 715, 777
EarI CTCTTC 3 cut(s) 408, 715, 777
EclXI CGGCCG 1 cut(s) 830
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 2 cut(s) 40, 253
Eco52I CGGCCG 1 cut(s) 830
Eco57I CTGAAG 2 cut(s) 600, 651
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 66, 75, 258
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 2 cut(s) 40, 253
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 49, 106
FaiI YATR 7 cut(s) 47, 104, 311, 441, 462, 464, 723
FaqI GGGAC 2 cut(s) 717, 746
FatI CATG 2 cut(s) 45, 102
FauI CCCGC 2 cut(s) 27, 240
Fnu4HI GCNGC 3 cut(s) 12, 150, 186
FokI GGATG 4 cut(s) 313, 431, 530, 690
FriOI GRGCYC 2 cut(s) 40, 253
Fsp4HI GCNGC 3 cut(s) 12, 150, 186
FspBI CTAG 2 cut(s) 407, 753
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 12, 150, 186
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 4 cut(s) 38, 74, 107, 832
HapII CCGG 4 cut(s) 17, 52, 626, 833
HgaI GACGC 1 cut(s) 373
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 2 cut(s) 49, 106
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 5 cut(s) 81, 392, 572, 749, 785
HpaII CCGG 4 cut(s) 17, 52, 626, 833
HphI GGTGA 2 cut(s) 274, 651
Hpy188I TCNGA 4 cut(s) 255, 331, 342, 397
Hpy188III TCNNGA 4 cut(s) 170, 553, 716, 789
HpyAV CCTTC 4 cut(s) 50, 488, 492, 694
HpyCH4III ACNGT 4 cut(s) 729, 737, 798, 820
HpyCH4V TGCA 1 cut(s) 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 17
HpyF3I CTNAG 5 cut(s) 202, 252, 283, 390, 513
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 49, 106
HspAI GCGC 1 cut(s) 14
KasI GGCGCC 1 cut(s) 13
KpnI GGTACC 1 cut(s) 67
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Kzo9I GATC 3 cut(s) 27, 502, 592
LmnI GCTCC 2 cut(s) 233, 385
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 407, 753
MaeIII GTNAC 3 cut(s) 262, 372, 634
MalI GATC 3 cut(s) 29, 504, 594
MbiI CCGCTC 2 cut(s) 34, 282
MboI GATC 3 cut(s) 27, 502, 592
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 40, 253
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 4 cut(s) 401, 566, 743, 794
Mox20I TGGCCA 1 cut(s) 107
MreI CGCCGGCG 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 16
MscI TGGCCA 1 cut(s) 107
MseI TTAA 5 cut(s) 300, 317, 359, 519, 612
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 4 cut(s) 17, 52, 626, 833
MspR9I CCNGG 5 cut(s) 53, 68, 77, 260, 626
MvaI CCWGG 3 cut(s) 68, 77, 260
MwoI GCNNNNNNNGC 2 cut(s) 11, 17
NaeI GCCGGC 1 cut(s) 18
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 2 cut(s) 53, 626
NcoI CCATGG 1 cut(s) 102
NdeII GATC 3 cut(s) 27, 502, 592
NgoMIV GCCGGC 1 cut(s) 16
NlaIII CATG 2 cut(s) 49, 106
NlaIV GGNNCC 6 cut(s) 15, 38, 65, 381, 594, 806
NmuCI GTSAC 2 cut(s) 262, 372
PasI CCCWGGG 1 cut(s) 76
PdiI GCCGGC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 81
PkrI GCNGC 3 cut(s) 13, 151, 187
PleI GAGTC 4 cut(s) 400, 566, 743, 793
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 4 cut(s) 400, 566, 743, 793
Psp6I CCWGG 3 cut(s) 66, 75, 258
PspGI CCWGG 3 cut(s) 66, 75, 258
PspN4I GGNNCC 6 cut(s) 15, 38, 65, 381, 594, 806
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 3 cut(s) 36, 37, 73
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 4 cut(s) 50, 65, 199, 278
RsaNI GTAC 4 cut(s) 49, 64, 198, 277
SaqAI TTAA 5 cut(s) 300, 317, 359, 519, 612
SatI GCNGC 3 cut(s) 12, 150, 186
Sau3AI GATC 3 cut(s) 27, 502, 592
Sau96I GGNCC 3 cut(s) 36, 37, 73
SchI GAGTC 4 cut(s) 401, 566, 743, 794
ScrFI CCNGG 5 cut(s) 53, 68, 77, 260, 626
SduI GDGCHC 2 cut(s) 40, 253
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 186
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 16
SmlI CTYRAG 3 cut(s) 161, 209, 551
SmoI CTYRAG 3 cut(s) 161, 209, 551
SsiI CCGC 5 cut(s) 11, 34, 150, 247, 280
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 2 cut(s) 407, 753
StyD4I CCNGG 5 cut(s) 51, 66, 75, 258, 624
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 4 cut(s) 729, 737, 798, 820
TaqI TCGA 2 cut(s) 30, 790
TauI GCSGC 2 cut(s) 14, 152
TfiI GAWTC 1 cut(s) 81
Tru1I TTAA 5 cut(s) 300, 317, 359, 519, 612
Tru9I TTAA 5 cut(s) 300, 317, 359, 519, 612
TscAI CASTG 4 cut(s) 210, 235, 381, 742
TseFI GTSAC 2 cut(s) 262, 372
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 2 cut(s) 262, 372
TspDTI ATGAA 2 cut(s) 435, 578
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 4 cut(s) 210, 235, 381, 742
XspI CTAG 2 cut(s) 407, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.