Rw5G016630

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010186
Physical Location & Seq
Reverse (-)
21122968 .. 21125419
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016630.1

Sequence Viewer

Length: 678 bp
ATGACATCGGCGGCGCCGGCGAACAAGATCGAGCGGGCCCACCTCATGTACCGGGAAGGGAGGTACCAGGAGGCCCTGGGATTCTACACCGAAGCCCTATCCATGGCCAAGACCAAACCCCAGAAGATTGCCCTCCACAGCAACAGAGCCGCTTGCTTCCTCAAGCTTCACGATTTCAAAAAGGCTGCAGAGGAATGTACCTCAGTGCTTGAGCTTGATTACAATCACACTGGAGCATTGATGTTGCGGGCTCAGACCCTGGGAACACTAAAGGAGTACCTTAAATCCATCATCCGAATGTATCAGAACCTTGAAGCCCGTTTAAGGACGCAAGTGTCACTGGCTCCAATACCCGAGTCCGAAGCAGAGCTAGAAGAAGAGGAAGAAGAAGAGGATGAAGCAGGGCCAAATAGATATGGGGGAGAGAAGAGCAATAATAGGAAAAGAAGATACTACTTCCCGGCTGAAGTTACACCAGATAAACATCAAGTATCACCTGAAAAAGAAAAGGGATGGCAAGCAATCCCAAAACCAAAGGGACACTCTTCTCTGGATTATGGAAGGTGGGACAGTGTGGAGGATGACTCTAGCGAAGATGATGACGATGATGATGAGGAAGAGTCTCGACCACAGTATCGGTTCCGTGTAAAAACTGTTGGTGTTCGGCCGGTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

225

Amino Acids

25.89

Weight (kDa)

5.43

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 11 - 64 7.9e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 13, 63
AccBSI CCGCTC 1 cut(s) 34
AciI CCGC 4 cut(s) 11, 34, 150, 247
AcoI YGGCCR 2 cut(s) 105, 665
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 4 cut(s) 50, 65, 199, 278
AfiI CCNNNNNNNGG 2 cut(s) 103, 324
AgsI TTSAA 2 cut(s) 178, 314
AjnI CCWGG 3 cut(s) 66, 75, 258
AluBI AGCT 3 cut(s) 166, 214, 370
AluI AGCT 3 cut(s) 166, 214, 370
Alw26I GTCTC 1 cut(s) 627
AlwNI CAGNNNCTG 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 5 cut(s) 36, 72, 105, 404, 665
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 4 cut(s) 36, 37, 73, 404
AsuC2I CCSGG 2 cut(s) 53, 461
AsuHPI GGTGA 1 cut(s) 486
AvaI CYCGRG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 617, 650
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 2 cut(s) 13, 63
BanII GRGCYC 2 cut(s) 40, 253
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 296, 507
BciT130I CCWGG 3 cut(s) 68, 77, 260
BcnI CCSGG 2 cut(s) 53, 461
BcoDI GTCTC 1 cut(s) 627
BfaI CTAG 2 cut(s) 371, 588
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 17
BisI GCNGC 3 cut(s) 12, 150, 186
BlsI GCNGC 3 cut(s) 13, 151, 187
Bme1390I CCNGG 5 cut(s) 53, 68, 77, 260, 461
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 4 cut(s) 36, 37, 73, 404
BmiI GGNNCC 5 cut(s) 15, 38, 65, 345, 641
BmrFI CCNGG 5 cut(s) 53, 68, 77, 260, 461
BplI GAGNNNNNCTC 2 cut(s) 569, 601
BpmI CTGGAG 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 146, 230
BpuMI CCSGG 2 cut(s) 53, 461
BsaBI GATNNNNATC 2 cut(s) 222, 483
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 5 cut(s) 75, 76, 102, 258, 259
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 324
Bse118I RCCGGY 2 cut(s) 16, 667
Bse1I ACTGG 2 cut(s) 235, 345
Bse8I GATNNNNATC 2 cut(s) 222, 483
BseBI CCWGG 3 cut(s) 68, 77, 260
BseDI CCNNGG 5 cut(s) 75, 76, 102, 258, 259
BseGI GGATG 4 cut(s) 291, 400, 518, 586
BseJI GATNNNNATC 2 cut(s) 222, 483
BseLI CCNNNNNNNGG 2 cut(s) 103, 324
BseMII CTCAG 2 cut(s) 216, 266
BseNI ACTGG 2 cut(s) 235, 345
BseSI GKGCMC 1 cut(s) 40
BseX3I CGGCCG 1 cut(s) 665
BseXI GCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 668
BshFI GGCC 5 cut(s) 38, 74, 107, 406, 667
BshNI GGYRCC 2 cut(s) 13, 63
BsiEI CGRYCG 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 4 cut(s) 17, 52, 461, 668
BslFI GGGAC 2 cut(s) 552, 581
BslI CCNNNNNNNGG 2 cut(s) 103, 324
BsmAI GTCTC 1 cut(s) 627
BsmFI GGGAC 2 cut(s) 552, 581
BsnI GGCC 5 cut(s) 38, 74, 107, 406, 667
BsoBI CYCGRG 1 cut(s) 353
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 40, 253
Bsp143I GATC 1 cut(s) 27
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 4 cut(s) 11, 34, 150, 247
BspANI GGCC 5 cut(s) 38, 74, 107, 406, 667
BspCNI CTCAG 2 cut(s) 215, 265
BspLI GGNNCC 5 cut(s) 15, 38, 65, 345, 641
BspMAI CTGCAG 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 422
BspT107I GGYRCC 2 cut(s) 13, 63
BsrBI CCGCTC 1 cut(s) 34
BsrFI RCCGGY 2 cut(s) 16, 667
BsrI ACTGG 2 cut(s) 235, 345
BssAI RCCGGY 2 cut(s) 16, 667
BssECI CCNNGG 5 cut(s) 75, 76, 102, 258, 259
BssMI GATC 1 cut(s) 27
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 68, 77, 260
Bst4CI ACNGT 3 cut(s) 572, 633, 655
Bst6I CTCTTC 5 cut(s) 372, 384, 422, 550, 612
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 5 cut(s) 18, 36, 154, 249, 519
BstDEI CTNAG 2 cut(s) 202, 252
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 4 cut(s) 291, 400, 518, 586
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 1 cut(s) 27
BstMCI CGRYCG 1 cut(s) 668
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 3 cut(s) 68, 77, 260
BstSCI CCNGG 5 cut(s) 51, 66, 75, 258, 459
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 172
BstZI CGGCCG 1 cut(s) 665
BsuRI GGCC 5 cut(s) 38, 74, 107, 406, 667
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 4 cut(s) 291, 400, 518, 586
BtsIMutI CAGTG 4 cut(s) 210, 228, 338, 577
Cac8I GCNNGC 5 cut(s) 18, 36, 154, 249, 519
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 2 cut(s) 16, 667
Cfr13I GGNCC 4 cut(s) 36, 37, 73, 404
CseI GACGC 1 cut(s) 337
Csp6I GTAC 4 cut(s) 49, 64, 198, 277
CviAII CATG 2 cut(s) 46, 103
CviQI GTAC 4 cut(s) 49, 64, 198, 277
DdeI CTNAG 2 cut(s) 202, 252
DinI GGCGCC 1 cut(s) 15
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 105, 665
EagI CGGCCG 1 cut(s) 665
Eam1104I CTCTTC 5 cut(s) 372, 384, 422, 550, 612
EarI CTCTTC 5 cut(s) 372, 384, 422, 550, 612
EclXI CGGCCG 1 cut(s) 665
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 2 cut(s) 40, 253
Eco52I CGGCCG 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 66, 75, 258
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 2 cut(s) 40, 253
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 49, 106
FaiI YATR 4 cut(s) 47, 104, 417, 558
FaqI GGGAC 2 cut(s) 552, 581
FatI CATG 2 cut(s) 45, 102
FauI CCCGC 2 cut(s) 27, 240
Fnu4HI GCNGC 3 cut(s) 12, 150, 186
FokI GGATG 4 cut(s) 278, 407, 525, 593
FriOI GRGCYC 2 cut(s) 40, 253
Fsp4HI GCNGC 3 cut(s) 12, 150, 186
FspBI CTAG 2 cut(s) 371, 588
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 12, 150, 186
GsuI CTGGAG 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 5 cut(s) 38, 74, 107, 406, 667
HapII CCGG 4 cut(s) 17, 52, 461, 668
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 2 cut(s) 49, 106
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 4 cut(s) 81, 356, 584, 620
HpaII CCGG 4 cut(s) 17, 52, 461, 668
HphI GGTGA 1 cut(s) 486
Hpy188I TCNGA 4 cut(s) 255, 296, 306, 361
Hpy188III TCNNGA 3 cut(s) 170, 551, 624
HpyAV CCTTC 2 cut(s) 50, 555
HpyCH4III ACNGT 3 cut(s) 572, 633, 655
HpyCH4V TGCA 1 cut(s) 188
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 2 cut(s) 202, 252
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 49, 106
HspAI GCGC 1 cut(s) 14
KasI GGCGCC 1 cut(s) 13
KpnI GGTACC 1 cut(s) 67
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 27
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 2 cut(s) 233, 349
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 2 cut(s) 371, 588
MaeIII GTNAC 2 cut(s) 336, 469
MalI GATC 1 cut(s) 29
MbiI CCGCTC 1 cut(s) 34
MboI GATC 1 cut(s) 27
MhlI GDGCHC 2 cut(s) 40, 253
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 3 cut(s) 365, 578, 629
Mox20I TGGCCA 1 cut(s) 107
MreI CGCCGGCG 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 16
MscI TGGCCA 1 cut(s) 107
MseI TTAA 2 cut(s) 282, 323
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 4 cut(s) 17, 52, 461, 668
MspR9I CCNGG 5 cut(s) 53, 68, 77, 260, 461
MvaI CCWGG 3 cut(s) 68, 77, 260
MwoI GCNNNNNNNGC 1 cut(s) 17
NaeI GCCGGC 1 cut(s) 18
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 2 cut(s) 53, 461
NcoI CCATGG 1 cut(s) 102
NdeII GATC 1 cut(s) 27
NgoMIV GCCGGC 1 cut(s) 16
NlaIII CATG 2 cut(s) 49, 106
NlaIV GGNNCC 5 cut(s) 15, 38, 65, 345, 641
NmuCI GTSAC 1 cut(s) 336
PasI CCCWGGG 2 cut(s) 76, 259
PciSI GCTCTTC 1 cut(s) 422
PdiI GCCGGC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 81
PkrI GCNGC 3 cut(s) 13, 151, 187
PleI GAGTC 3 cut(s) 364, 578, 628
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 3 cut(s) 364, 578, 628
Psp6I CCWGG 3 cut(s) 66, 75, 258
PspGI CCWGG 3 cut(s) 66, 75, 258
PspN4I GGNNCC 5 cut(s) 15, 38, 65, 345, 641
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 4 cut(s) 36, 37, 73, 404
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 4 cut(s) 50, 65, 199, 278
RsaNI GTAC 4 cut(s) 49, 64, 198, 277
RseI CAYNNNNRTG 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 2 cut(s) 282, 323
SatI GCNGC 3 cut(s) 12, 150, 186
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 4 cut(s) 36, 37, 73, 404
SchI GAGTC 3 cut(s) 365, 578, 629
ScrFI CCNGG 5 cut(s) 53, 68, 77, 260, 461
SduI GDGCHC 2 cut(s) 40, 253
SfcI CTRYAG 1 cut(s) 186
SfoI GGCGCC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 2 cut(s) 161, 209
SmoI CTYRAG 2 cut(s) 161, 209
SsiI CCGC 4 cut(s) 11, 34, 150, 247
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 2 cut(s) 371, 588
StyD4I CCNGG 5 cut(s) 51, 66, 75, 258, 459
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 3 cut(s) 572, 633, 655
TaqI TCGA 2 cut(s) 30, 625
TauI GCSGC 2 cut(s) 14, 152
TfiI GAWTC 1 cut(s) 81
Tru1I TTAA 2 cut(s) 282, 323
Tru9I TTAA 2 cut(s) 282, 323
TscAI CASTG 4 cut(s) 210, 235, 345, 577
TseFI GTSAC 1 cut(s) 336
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 336
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 4 cut(s) 210, 235, 345, 577
XspI CTAG 2 cut(s) 371, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.