pycom05g20530

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010186
Physical Location & Seq
Forward (+)
23314228 .. 23315827
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20530.3

Sequence Viewer

Length: 846 bp
ATGCGGGCCCACCGGATGTACCGGGAAGGTAGCTACTCGGAGGCGCTAGGGTTTTACACCGAAGCCCTGTCCATGGCCAGGACCAAACCCCAGAAGATCGCCCTCCGCAGCAACCGAGCTGCTTGCTTCTTGAAGCTCCACGATTTCAAAAAGGCCGCAGATGAATGTACCTCAGTTCTTGAGCTTGATTACAATCACACTGGAGCACTGATGTTGCGGGCGCAGACCCTGGTGACCCTCAAGGAATATCACTCAGCGCTCTTTGATGTTCATCGACTTATCGAGCTAAATCCATCATCGGAAGTTTATCAAAACCTTCAAGCCCGTCTAAAGACGCAAGTGTCACTTGCTCCAATACCTGAATCCGAAGCAGAGCTAGAAGAAGAGGAAGACGAGGAGGAGGATGAAGTAGAGCCAGATAGATATCAGGAGAAAGAGGAGCAATACAAAGAAAAAGAAGATGTATTTTCAGGTGCACGAAGGGATCAGATTCCTGAGGTTAATGAGACAACTGCTGTCGCCAATGTGATTCCCCCTAAGACACCAGTAAACAGGGAAGTTTCTCCACAGGGAAGGGATCCAAAAGGTAACTCGATAAAGACCACTTCCCCAGCTGAAGTTGTTGCCCCTCGTGCAGAGCACGTCAAGGCATCGTCTGAAAAAGACAAAGACAAAGGATGGCAAGCAATCCCGAAACCAAAGGGACACTCTTCTCTGGACTATGCACGGTGGGACAGAGTGGAAGATGACTCTAGTGAAGATGATGATGATGACGATGACGATGAGGACTCTCAACCTCAGTATAGATTCCGTGTAAAAACTGTTGGTGTCCGGCCAGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

282

Amino Acids

32.07

Weight (kDa)

4.92

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 340
AciI CCGC 4 cut(s) 4, 106, 156, 217
AclWI GGATC 3 cut(s) 492, 572, 585
AcoI YGGCCR 2 cut(s) 75, 833
AcuI CTGAAG 1 cut(s) 636
AfaI GTAC 2 cut(s) 20, 169
AfeI AGCGCT 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 73, 78
AgsI TTSAA 3 cut(s) 133, 148, 320
AjiI CACGTC 1 cut(s) 643
AjnI CCWGG 2 cut(s) 77, 228
AluBI AGCT 7 cut(s) 33, 119, 136, 184, 286, 376, 614
AluI AGCT 7 cut(s) 33, 119, 136, 184, 286, 376, 614
Alw21I GWGCWC 3 cut(s) 208, 478, 642
Alw26I GTCTC 1 cut(s) 500
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 3 cut(s) 492, 572, 585
AlwNI CAGNNNCTG 1 cut(s) 229
Aor51HI AGCGCT 1 cut(s) 258
AoxI GGCC 4 cut(s) 6, 75, 153, 833
ApaI GGGCCC 1 cut(s) 10
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 2 cut(s) 108, 119
AspLEI GCGC 3 cut(s) 46, 223, 259
AspS9I GGNCC 3 cut(s) 6, 7, 81
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 1 cut(s) 81
AxyI CCTNAGG 1 cut(s) 495
BaeGI GKGCMC 2 cut(s) 10, 478
BalI TGGCCA 1 cut(s) 77
BamHI GGATCC 1 cut(s) 577
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 3 cut(s) 208, 478, 642
BbvI GCAGC 2 cut(s) 106, 120
BccI CCATC 2 cut(s) 301, 672
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 2 cut(s) 79, 230
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 3 cut(s) 47, 377, 753
BfoI RGCGCY 2 cut(s) 47, 260
BisI GCNGC 3 cut(s) 109, 120, 156
BlsI GCNGC 3 cut(s) 110, 121, 157
Bme1390I CCNGG 3 cut(s) 23, 79, 230
Bme18I GGWCC 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 643
BmgT120I GGNCC 3 cut(s) 6, 7, 81
BmiI GGNNCC 2 cut(s) 8, 579
BmrFI CCNGG 3 cut(s) 23, 79, 230
BmsI GCATC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 396
BpmI CTGGAG 1 cut(s) 222
BpuEI CTTGAG 2 cut(s) 200, 224
BpuMI CCSGG 1 cut(s) 23
BsaBI GATNNNNATC 3 cut(s) 192, 270, 423
BsaJI CCNNGG 2 cut(s) 72, 228
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 78
Bse1I ACTGG 3 cut(s) 205, 545, 836
Bse21I CCTNAGG 1 cut(s) 495
Bse8I GATNNNNATC 3 cut(s) 192, 270, 423
BseBI CCWGG 2 cut(s) 79, 230
BseDI CCNNGG 2 cut(s) 72, 228
BseGI GGATG 3 cut(s) 21, 409, 683
BseJI GATNNNNATC 3 cut(s) 192, 270, 423
BseLI CCNNNNNNNGG 2 cut(s) 73, 78
BseMII CTCAG 4 cut(s) 186, 267, 486, 812
BseNI ACTGG 3 cut(s) 205, 545, 836
BseRI GAGGAG 3 cut(s) 410, 413, 452
BseSI GKGCMC 2 cut(s) 10, 478
BseXI GCAGC 2 cut(s) 106, 120
BseYI CCCAGC 1 cut(s) 610
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 4 cut(s) 8, 77, 155, 835
BsiHKAI GWGCWC 3 cut(s) 208, 478, 642
BsiSI CCGG 3 cut(s) 13, 22, 832
BslFI GGGAC 2 cut(s) 717, 746
BslI CCNNNNNNNGG 2 cut(s) 73, 78
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 717, 746
BsnI GGCC 4 cut(s) 8, 77, 155, 835
Bsp120I GGGCCC 1 cut(s) 6
Bsp1286I GDGCHC 4 cut(s) 10, 208, 478, 642
Bsp143I GATC 3 cut(s) 96, 484, 577
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 4 cut(s) 4, 106, 156, 217
BspANI GGCC 4 cut(s) 8, 77, 155, 835
BspCNI CTCAG 4 cut(s) 185, 266, 487, 811
BspLI GGNNCC 2 cut(s) 8, 579
BspPI GGATC 3 cut(s) 492, 572, 585
BsrI ACTGG 3 cut(s) 205, 545, 836
BssECI CCNNGG 2 cut(s) 72, 228
BssMI GATC 3 cut(s) 96, 484, 577
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 79, 230
Bst4CI ACNGT 2 cut(s) 729, 823
Bst6I CTCTTC 2 cut(s) 378, 715
BstC8I GCNNGC 4 cut(s) 6, 124, 219, 684
BstDEI CTNAG 5 cut(s) 172, 253, 495, 537, 798
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 232
BstF5I GGATG 3 cut(s) 21, 409, 683
BstH2I RGCGCY 2 cut(s) 47, 260
BstHHI GCGC 3 cut(s) 46, 223, 259
BstKTI GATC 3 cut(s) 99, 487, 580
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 3 cut(s) 96, 484, 577
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 2 cut(s) 79, 230
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 21, 77, 228
BstSLI GKGCMC 2 cut(s) 10, 478
BstV1I GCAGC 2 cut(s) 106, 120
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 4 cut(s) 8, 77, 155, 835
BtgI CCRYGG 1 cut(s) 72
BtrI CACGTC 1 cut(s) 643
BtsCI GGATG 3 cut(s) 21, 409, 683
BtsIMutI CAGTG 3 cut(s) 198, 206, 843
Cac8I GCNNGC 4 cut(s) 6, 124, 219, 684
CaiI CAGNNNCTG 1 cut(s) 229
CfoI GCGC 3 cut(s) 46, 223, 259
Cfr13I GGNCC 3 cut(s) 6, 7, 81
CseI GACGC 1 cut(s) 343
Csp6I GTAC 2 cut(s) 19, 168
CviAII CATG 1 cut(s) 73
CviQI GTAC 2 cut(s) 19, 168
DdeI CTNAG 5 cut(s) 172, 253, 495, 537, 798
DpnI GATC 3 cut(s) 98, 486, 579
DpnII GATC 3 cut(s) 96, 484, 577
DrdI GACNNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 340
EaeI YGGCCR 2 cut(s) 75, 833
Eam1104I CTCTTC 2 cut(s) 378, 715
EarI CTCTTC 2 cut(s) 378, 715
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 81
Eco47III AGCGCT 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 636
Eco81I CCTNAGG 1 cut(s) 495
Eco91I GGTNACC 1 cut(s) 232
EcoO65I GGTNACC 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 77, 228
EcoRV GATATC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 1 cut(s) 76
FaiI YATR 3 cut(s) 74, 723, 804
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 2 cut(s) 717, 746
FatI CATG 1 cut(s) 72
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 109, 120, 156
FokI GGATG 3 cut(s) 28, 416, 690
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 3 cut(s) 109, 120, 156
FspBI CTAG 3 cut(s) 47, 377, 753
GlaI GCGC 3 cut(s) 45, 222, 258
GluI GCNGC 3 cut(s) 109, 120, 156
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 2 cut(s) 47, 260
HaeIII GGCC 4 cut(s) 8, 77, 155, 835
HapII CCGG 3 cut(s) 13, 22, 832
HgaI GACGC 1 cut(s) 343
HhaI GCGC 3 cut(s) 46, 223, 259
Hin1II CATG 1 cut(s) 76
Hin6I GCGC 3 cut(s) 44, 221, 257
HinP1I GCGC 3 cut(s) 44, 221, 257
HinfI GANTC 6 cut(s) 362, 490, 529, 749, 788, 807
HpaII CCGG 3 cut(s) 13, 22, 832
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 2 cut(s) 476, 550
Hpy188I TCNGA 5 cut(s) 40, 301, 367, 489, 658
Hpy188III TCNNGA 6 cut(s) 130, 179, 428, 494, 691, 716
Hpy8I GTNNAC 2 cut(s) 476, 550
HpyAV CCTTC 4 cut(s) 20, 326, 474, 567
HpyCH4III ACNGT 2 cut(s) 729, 823
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 3 cut(s) 476, 635, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 5 cut(s) 172, 253, 495, 537, 798
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 1 cut(s) 76
HspAI GCGC 3 cut(s) 44, 221, 257
Kzo9I GATC 3 cut(s) 96, 484, 577
LmnI GCTCC 4 cut(s) 141, 203, 355, 439
Lsp1109I GCAGC 2 cut(s) 106, 120
LweI GCATC 1 cut(s) 659
MaeI CTAG 3 cut(s) 47, 377, 753
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 232, 342, 587
MalI GATC 3 cut(s) 98, 486, 579
MboI GATC 3 cut(s) 96, 484, 577
MboII GAAGA 8 cut(s) 106, 392, 395, 401, 470, 702, 755, 770
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 4 cut(s) 10, 208, 478, 642
MlsI TGGCCA 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 2 cut(s) 743, 782
Mox20I TGGCCA 1 cut(s) 77
MscI TGGCCA 1 cut(s) 77
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 3 cut(s) 13, 22, 832
MspR9I CCNGG 3 cut(s) 23, 79, 230
MvaI CCWGG 2 cut(s) 79, 230
MwoI GCNNNNNNNGC 1 cut(s) 632
NciI CCSGG 1 cut(s) 23
NcoI CCATGG 1 cut(s) 72
NdeII GATC 3 cut(s) 96, 484, 577
NlaIII CATG 1 cut(s) 76
NlaIV GGNNCC 2 cut(s) 8, 579
NmuCI GTSAC 2 cut(s) 232, 342
PfeI GAWTC 4 cut(s) 362, 490, 529, 807
PkrI GCNGC 3 cut(s) 110, 121, 157
PleI GAGTC 2 cut(s) 743, 782
PpsI GAGTC 2 cut(s) 743, 782
Psp6I CCWGG 2 cut(s) 77, 228
PspEI GGTNACC 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 2 cut(s) 77, 228
PspN4I GGNNCC 2 cut(s) 8, 579
PspOMI GGGCCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 6, 7, 81
PstNI CAGNNNCTG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 20, 169
RsaNI GTAC 2 cut(s) 19, 168
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 3 cut(s) 109, 120, 156
Sau3AI GATC 3 cut(s) 96, 484, 577
Sau96I GGNCC 3 cut(s) 6, 7, 81
SchI GAGTC 2 cut(s) 743, 782
ScrFI CCNGG 3 cut(s) 23, 79, 230
SduI GDGCHC 4 cut(s) 10, 208, 478, 642
SfaNI GCATC 1 cut(s) 659
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 179, 239
SmoI CTYRAG 2 cut(s) 179, 239
SsiI CCGC 4 cut(s) 4, 106, 156, 217
SspMI CTAG 3 cut(s) 47, 377, 753
StyD4I CCNGG 3 cut(s) 21, 77, 228
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 729, 823
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 274, 282, 593
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 4 cut(s) 362, 490, 529, 807
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 3 cut(s) 205, 213, 843
TseFI GTSAC 2 cut(s) 232, 342
TseI GCWGC 2 cut(s) 108, 119
Tsp45I GTSAC 2 cut(s) 232, 342
TspDTI ATGAA 3 cut(s) 177, 260, 420
TspGWI ACGGA 1 cut(s) 800
TspRI CASTG 3 cut(s) 205, 213, 843
VneI GTGCAC 1 cut(s) 474
VpaK11BI GGWCC 1 cut(s) 81
XspI CTAG 3 cut(s) 47, 377, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.