MD05G1223200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012212
Physical Location & Seq
Forward (+)
35569555 .. 35570163
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1223200.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCCGACGGGAGAACAAACCAATCGATCATGGAGCCAAAGCCCTCACACCCACTTCACCAAATCGCTCAGTCGCCGAGCCACAAGCTTCTCCTGAAGCAATGGCTGAAAGAAGAAGAGCTAAATCTCAACAGGGTCTCTCTCAAAGAATCCCAAATCGACTCGGTTCGAAAGGAAATCACGATTCTTTTCATTATCTTCTTCCTCTTCCACTCCACGGCCCTCACCCTTCTTTTCAGTTCCTCATCGTGGGACCCGCGCGGGTTCGCATGCCGCCGGTCCTGGATCCCTTCGCTCTGCTCGCTCTGCTTCTCACTGGGGATCATTTGGGCGATTAGGTATAAAACCGACACGGAGGGGCACCTGGAGAAGTTGTTGGAGAGAGAGAAAGAGGATAAGAATCTTCTGGGAAAGTGCATCGAGGAGCTGAAGAAGAAAGGGTTGGAGTTCGATTTGTTAAAGGAGGTGGACGCGCTGAGGAGGGCCAAGAGCTTGAGAGTCGAAGCGAAGGCAGTAAGGAAGTGGAATGCGAGGGATTTTGTGACACTGTTTTTCTTCACTGTGTCTTGCTTTGTGATCATTGTCACAAGGGTTATTTTGTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

23.48

Weight (kDa)

9.28

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12870
fragaria_vesca FvH4_3g15460
malus_domestica MD01G1075700.v1.1 MD05G1223200.v1.1 MD10G1204600.v1.1 MD12G1131000.v1.1
prunus_persica Prupe.4G137100_v2.0.a1
pyrus_communis pycom05g20560 pycom10g17530
rosa_chinensis RchiOBHm_Chr5g0025961
rosa_laevigata RLG00000032887
rosa_multiflora Rmu_sc0003020.1_g000010
rosa_roxburghii Rroxscaffold_1G00053280
rosa_rugosa Rorug05G0089900
rosa_samantha Rh5AG181700 Rh5BG179800 Rh5CG198200 Rh5DG180700
rosa_wichuraiana Rw5G016530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 360
AccII CGCG 3 cut(s) 259, 261, 473
AciI CCGC 3 cut(s) 257, 261, 274
AclWI GGATC 3 cut(s) 280, 293, 329
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 116, 449
AfiI CCNNNNNNNGG 2 cut(s) 217, 249
AjnI CCWGG 2 cut(s) 281, 363
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 4 cut(s) 88, 121, 427, 492
AluI AGCT 4 cut(s) 88, 121, 427, 492
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 3 cut(s) 280, 293, 329
AoxI GGCC 3 cut(s) 3, 219, 483
AspLEI GCGC 2 cut(s) 261, 475
AspS9I GGNCC 4 cut(s) 220, 253, 279, 483
AsuHPI GGTGA 2 cut(s) 50, 217
AsuII TTCGAA 1 cut(s) 169
AvaII GGWCC 2 cut(s) 253, 279
BaeGI GKGCMC 1 cut(s) 363
BamHI GGATCC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 360
BbvCI CCTCAGC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 2 cut(s) 283, 365
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 607
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme1390I CCNGG 2 cut(s) 283, 365
Bme18I GGWCC 2 cut(s) 253, 279
BmgT120I GGNCC 4 cut(s) 220, 253, 279, 483
BmiI GGNNCC 5 cut(s) 36, 254, 255, 287, 362
BmrFI CCNGG 2 cut(s) 283, 365
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 426
BmuI ACTGGG 1 cut(s) 326
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 476
Bpu14I TTCGAA 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 514
Bsa29I ATCGAT 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 249
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 2 cut(s) 283, 365
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 217, 249
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 2 cut(s) 83, 467
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 2 cut(s) 437, 493
BseSI GKGCMC 1 cut(s) 363
Bsh1236I CGCG 3 cut(s) 259, 261, 473
BshFI GGCC 3 cut(s) 5, 221, 485
BshNI GGYRCC 1 cut(s) 360
BshVI ATCGAT 1 cut(s) 26
BsiSI CCGG 1 cut(s) 277
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 217, 249
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 3 cut(s) 5, 221, 485
Bso31I GGTCTC 1 cut(s) 142
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 4 cut(s) 27, 285, 321, 576
BspACI CCGC 3 cut(s) 257, 261, 274
BspANI GGCC 3 cut(s) 5, 221, 485
BspCNI CTCAG 2 cut(s) 82, 468
BspDI ATCGAT 1 cut(s) 26
BspFNI CGCG 3 cut(s) 259, 261, 473
BspLI GGNNCC 5 cut(s) 36, 254, 255, 287, 362
BspPI GGATC 3 cut(s) 280, 293, 329
BspQI GCTCTTC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 360
BspTNI GGTCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 4 cut(s) 27, 285, 321, 576
Bst2UI CCWGG 2 cut(s) 283, 365
Bst4CI ACNGT 2 cut(s) 549, 562
Bst6I CTCTTC 2 cut(s) 111, 212
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 271, 302
BstDEI CTNAG 2 cut(s) 69, 476
BstDSI CCRYGG 1 cut(s) 216
BstFNI CGCG 3 cut(s) 259, 261, 473
BstHHI GCGC 2 cut(s) 261, 475
BstKTI GATC 4 cut(s) 30, 288, 324, 579
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 4 cut(s) 27, 285, 321, 576
BstMWI GCNNNNNNNGC 2 cut(s) 301, 306
BstNI CCWGG 2 cut(s) 283, 365
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 281, 363
BstSLI GKGCMC 1 cut(s) 363
BstUI CGCG 3 cut(s) 259, 261, 473
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 3 cut(s) 5, 221, 485
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 216
BtsIMutI CAGTG 3 cut(s) 314, 545, 558
Cac8I GCNNGC 2 cut(s) 271, 302
CfoI GCGC 2 cut(s) 261, 475
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 4 cut(s) 220, 253, 279, 483
ClaI ATCGAT 1 cut(s) 26
CseI GACGC 1 cut(s) 479
CviAII CATG 2 cut(s) 31, 270
DdeI CTNAG 2 cut(s) 69, 476
DpnI GATC 4 cut(s) 29, 287, 323, 578
DpnII GATC 4 cut(s) 27, 285, 321, 576
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 111, 212
EarI CTCTTC 2 cut(s) 111, 212
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 253, 279
Eco57I CTGAAG 2 cut(s) 116, 449
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 281, 363
FaeI CATG 2 cut(s) 34, 273
FaiI YATR 3 cut(s) 32, 271, 342
FaqI GGGAC 1 cut(s) 266
FatI CATG 2 cut(s) 30, 269
FauI CCCGC 2 cut(s) 254, 264
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 1 cut(s) 274
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 1 cut(s) 607
GlaI GCGC 2 cut(s) 260, 474
GluI GCNGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 3 cut(s) 5, 221, 485
HapII CCGG 1 cut(s) 277
HgaI GACGC 1 cut(s) 479
HhaI GCGC 2 cut(s) 261, 475
Hin1II CATG 2 cut(s) 34, 273
Hin6I GCGC 2 cut(s) 259, 473
HinP1I GCGC 2 cut(s) 259, 473
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 5 cut(s) 149, 161, 184, 400, 498
HpaII CCGG 1 cut(s) 277
HphI GGTGA 2 cut(s) 50, 217
Hpy166II GTNNAC 1 cut(s) 469
Hpy188III TCNNGA 2 cut(s) 94, 181
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 239, 300, 502
HpyCH4III ACNGT 2 cut(s) 549, 562
HpyCH4V TGCA 1 cut(s) 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 306
HpyF3I CTNAG 2 cut(s) 69, 476
Hsp92II CATG 2 cut(s) 34, 273
HspAI GCGC 2 cut(s) 259, 473
KflI GGGWCCC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 4 cut(s) 27, 285, 321, 576
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 34, 424
LpnPI CCDG 9 cut(s) 107, 118, 268, 290, 295, 302, 350, 377, 392
LweI GCATC 1 cut(s) 426
MaeI CTAG 1 cut(s) 607
MaeIII GTNAC 3 cut(s) 541, 583, 602
MalI GATC 4 cut(s) 29, 287, 323, 578
MboI GATC 4 cut(s) 27, 285, 321, 576
MboII GAAGA 9 cut(s) 125, 128, 190, 193, 199, 395, 442, 445, 547
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 363
MlyI GAGTC 2 cut(s) 155, 507
MmeI TCCRAC 2 cut(s) 357, 423
MseI TTAA 1 cut(s) 458
MspI CCGG 1 cut(s) 277
MspR9I CCNGG 2 cut(s) 283, 365
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 2 cut(s) 283, 365
MvnI CGCG 3 cut(s) 259, 261, 473
MwoI GCNNNNNNNGC 2 cut(s) 301, 306
NdeII GATC 4 cut(s) 27, 285, 321, 576
NlaIII CATG 2 cut(s) 34, 273
NlaIV GGNNCC 5 cut(s) 36, 254, 255, 287, 362
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 2 cut(s) 541, 583
NspI RCATGY 1 cut(s) 273
NspV TTCGAA 1 cut(s) 169
PaeI GCATGC 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 111
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 3 cut(s) 149, 184, 400
PfoI TCCNGGA 1 cut(s) 281
PkrI GCNGC 1 cut(s) 275
PleI GAGTC 2 cut(s) 155, 506
PpsI GAGTC 2 cut(s) 155, 506
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 281, 363
PspGI CCWGG 2 cut(s) 281, 363
PspN4I GGNNCC 5 cut(s) 36, 254, 255, 287, 362
PspPI GGNCC 4 cut(s) 220, 253, 279, 483
PspPPI RGGWCCY 1 cut(s) 253
PsuI RGATCY 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 4 cut(s) 27, 285, 321, 576
Sau96I GGNCC 4 cut(s) 220, 253, 279, 483
SchI GAGTC 2 cut(s) 155, 507
ScrFI CCNGG 2 cut(s) 283, 365
SduI GDGCHC 1 cut(s) 363
SetI ASST 7 cut(s) 90, 123, 341, 366, 429, 468, 494
SfaNI GCATC 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 2 cut(s) 253, 279
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
SphI GCATGC 1 cut(s) 273
SsiI CCGC 3 cut(s) 257, 261, 274
SspMI CTAG 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 281, 363
TaaI ACNGT 2 cut(s) 549, 562
TaqI TCGA 6 cut(s) 26, 159, 169, 420, 450, 501
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 3 cut(s) 149, 184, 400
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 3 cut(s) 321, 552, 565
TseFI GTSAC 2 cut(s) 541, 583
Tsp45I GTSAC 2 cut(s) 541, 583
TspDTI ATGAA 1 cut(s) 181
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 3 cut(s) 321, 552, 565
VpaK11BI GGWCC 2 cut(s) 253, 279
XceI RCATGY 1 cut(s) 273
XspI CTAG 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.