pycom10g17530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012212
Physical Location & Seq
Forward (+)
20755690 .. 20756830
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17530.3

Sequence Viewer

Length: 678 bp
ATGAAAATAGAGTTCACTATCACAGATCTGATGATCTGGAATAATCCTAGCCGTTACAAAAGCTCGCGATCTCGTGAATACCCGCATACGAAATCGATCATGGAGCCGAAGTTCTCACACCCACTTCACCAAATCGCTCAGTCTCCGAGCCACAAGCTTCTCCTGAAGCAATGGCTGAAAGAAGAAGAGCTAATCCTCAACAGGGTCTCTCTCAAGGAGTCCCAAATCGACTCAGTTCGAAAGGAGATCACGATGCTCTTCATCTTCTTCTTCCTCTTCCACTCCACGGCTCTCACCCTTCTCTTCAGTTCCTCGTCGAGGGACCCGCACGGGTTCGCGTGCCGCAGGTCCTGGATCCCGTCGCTCTGCTCTCTCTGCTTCTCGCTGGGGATCATTTGGGCGATAAGGTATAAAAGCGACACGGAGGGGCACCTGGAGAAGTTGTTGGAGAGAGAAAAGGAGGATAAGAACCTGCTGGGAAAGTGCATTGAGGAGCTGAAGAAGAAGGGGTTGGAGTTCGATTTGTTGAAGGAGGTTGACGCGCTGAGGAGGGCCAAGAGTTTGAGAGTCGAAGCAAAGGCGGTGAGGAAGTGGAATGCGAGGGATTTTGTGACAATGTTTTTCTTCACTGTGTCTTGCTTTGTGATCATTGTCACCAGAGTTATTTTGTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

26.48

Weight (kDa)

9.47

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12870
fragaria_vesca FvH4_3g15460
malus_domestica MD01G1075700.v1.1 MD05G1223200.v1.1 MD10G1204600.v1.1 MD12G1131000.v1.1
prunus_persica Prupe.4G137100_v2.0.a1
pyrus_communis pycom05g20560 pycom10g17530
rosa_chinensis RchiOBHm_Chr5g0025961
rosa_laevigata RLG00000032887
rosa_multiflora Rmu_sc0003020.1_g000010
rosa_roxburghii Rroxscaffold_1G00053280
rosa_rugosa Rorug05G0089900
rosa_samantha Rh5AG181700 Rh5BG179800 Rh5CG198200 Rh5DG180700
rosa_wichuraiana Rw5G016530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 336, 480
AccB1I GGYRCC 1 cut(s) 429
AccII CGCG 3 cut(s) 67, 338, 542
AciI CCGC 4 cut(s) 83, 326, 343, 581
AclWI GGATC 3 cut(s) 349, 362, 398
AcuI CTGAAG 3 cut(s) 185, 289, 518
AfiI CCNNNNNNNGG 3 cut(s) 202, 286, 318
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 2 cut(s) 350, 432
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 4 cut(s) 63, 157, 190, 496
AluI AGCT 4 cut(s) 63, 157, 190, 496
Alw26I GTCTC 2 cut(s) 147, 211
AlwI GGATC 3 cut(s) 349, 362, 398
AlwNI CAGNNNCTG 1 cut(s) 351
AoxI GGCC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 322, 348, 552
AsuHPI GGTGA 4 cut(s) 119, 286, 595, 646
AsuII TTCGAA 1 cut(s) 238
AvaII GGWCC 2 cut(s) 322, 348
BaeGI GKGCMC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 354
BanI GGYRCC 1 cut(s) 429
BauI CACGAG 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 545
BceAI ACGGC 2 cut(s) 36, 303
BciT130I CCWGG 2 cut(s) 352, 434
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 147, 211
BfaI CTAG 2 cut(s) 48, 676
BfuAI ACCTGC 2 cut(s) 336, 480
BglII AGATCT 1 cut(s) 25
BisI GCNGC 1 cut(s) 343
BlsI GCNGC 1 cut(s) 344
Bme1390I CCNGG 2 cut(s) 352, 434
Bme18I GGWCC 2 cut(s) 322, 348
BmgT120I GGNCC 3 cut(s) 322, 348, 552
BmiI GGNNCC 5 cut(s) 105, 323, 324, 356, 431
BmrFI CCNGG 2 cut(s) 352, 434
BmsI GCATC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 197
Bsa29I ATCGAT 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 285
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 286, 318
Bse3DI GCAATG 1 cut(s) 176
BseBI CCWGG 2 cut(s) 352, 434
BseCI ATCGAT 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 285
BseLI CCNNNNNNNGG 3 cut(s) 202, 286, 318
BseMI GCAATG 1 cut(s) 176
BseMII CTCAG 3 cut(s) 152, 246, 536
BseRI GAGGAG 2 cut(s) 506, 562
BseSI GKGCMC 1 cut(s) 432
BseYI CCCAGC 2 cut(s) 385, 475
Bsh1236I CGCG 3 cut(s) 67, 338, 542
BshFI GGCC 1 cut(s) 554
BshNI GGYRCC 1 cut(s) 429
BshVI ATCGAT 1 cut(s) 95
BslFI GGGAC 2 cut(s) 205, 335
BslI CCNNNNNNNGG 3 cut(s) 202, 286, 318
BsmAI GTCTC 2 cut(s) 147, 211
BsmFI GGGAC 2 cut(s) 205, 335
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 1 cut(s) 554
Bso31I GGTCTC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
Bsp68I TCGCGA 1 cut(s) 67
BspACI CCGC 4 cut(s) 83, 326, 343, 581
BspANI GGCC 1 cut(s) 554
BspCNI CTCAG 3 cut(s) 151, 245, 537
BspDI ATCGAT 1 cut(s) 95
BspFNI CGCG 3 cut(s) 67, 338, 542
BspLI GGNNCC 5 cut(s) 105, 323, 324, 356, 431
BspMI ACCTGC 2 cut(s) 336, 480
BspPI GGATC 3 cut(s) 349, 362, 398
BspQI GCTCTTC 2 cut(s) 180, 263
BspT104I TTCGAA 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
BssSI CACGAG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 352, 434
Bst4CI ACNGT 1 cut(s) 631
Bst6I CTCTTC 4 cut(s) 180, 263, 281, 308
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 65, 340
BstDEI CTNAG 3 cut(s) 138, 232, 545
BstDSI CCRYGG 1 cut(s) 285
BstENI CCTNNNNNAGG 1 cut(s) 316
BstFNI CGCG 3 cut(s) 67, 338, 542
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 8 cut(s) 28, 36, 71, 99, 249, 357, 393, 648
BstMAI GTCTC 2 cut(s) 147, 211
BstMBI GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 2 cut(s) 352, 434
BstSCI CCNGG 2 cut(s) 350, 432
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 3 cut(s) 67, 338, 542
BstX2I RGATCY 2 cut(s) 25, 354
BstYI RGATCY 2 cut(s) 25, 354
Bsu15I ATCGAT 1 cut(s) 95
BsuRI GGCC 1 cut(s) 554
BsuTUI ATCGAT 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 627
BtuMI TCGCGA 1 cut(s) 67
BveI ACCTGC 2 cut(s) 336, 480
Cac8I GCNNGC 2 cut(s) 65, 340
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 3 cut(s) 322, 348, 552
ClaI ATCGAT 1 cut(s) 95
CseI GACGC 1 cut(s) 548
CviAII CATG 1 cut(s) 100
DdeI CTNAG 3 cut(s) 138, 232, 545
DpnI GATC 8 cut(s) 27, 35, 70, 98, 248, 356, 392, 647
DpnII GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
Eam1104I CTCTTC 4 cut(s) 180, 263, 281, 308
EarI CTCTTC 4 cut(s) 180, 263, 281, 308
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 322, 348
Eco57I CTGAAG 3 cut(s) 185, 289, 518
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoO109I RGGNCCY 2 cut(s) 322, 348
EcoRII CCWGG 2 cut(s) 350, 432
FaeI CATG 1 cut(s) 103
FaiI YATR 3 cut(s) 87, 101, 411
FaqI GGGAC 2 cut(s) 205, 335
FatI CATG 1 cut(s) 99
FauI CCCGC 2 cut(s) 90, 333
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 1 cut(s) 343
Fsp4HI GCNGC 1 cut(s) 343
FspBI CTAG 2 cut(s) 48, 676
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 343
GsaI CCCAGC 2 cut(s) 389, 479
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 1 cut(s) 554
HgaI GACGC 1 cut(s) 548
HhaI GCGC 1 cut(s) 544
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 3 cut(s) 218, 230, 567
HphI GGTGA 4 cut(s) 119, 286, 595, 646
Hpy166II GTNNAC 2 cut(s) 15, 538
Hpy188I TCNGA 2 cut(s) 30, 147
Hpy188III TCNNGA 5 cut(s) 37, 66, 74, 163, 250
Hpy8I GTNNAC 2 cut(s) 15, 538
Hpy99I CGWCG 2 cut(s) 319, 364
HpyAV CCTTC 3 cut(s) 308, 499, 523
HpyCH4III ACNGT 1 cut(s) 631
HpyCH4V TGCA 1 cut(s) 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 3 cut(s) 138, 232, 545
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 1 cut(s) 542
KflI GGGWCCC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
LguI GCTCTTC 2 cut(s) 180, 263
LmnI GCTCC 2 cut(s) 103, 493
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 48, 676
MaeIII GTNAC 4 cut(s) 53, 610, 652, 671
MalI GATC 8 cut(s) 27, 35, 70, 98, 248, 356, 392, 647
MboI GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
MflI RGATCY 2 cut(s) 25, 354
MhlI GDGCHC 1 cut(s) 432
MlyI GAGTC 3 cut(s) 224, 227, 576
MmeI TCCRAC 2 cut(s) 426, 492
MspR9I CCNGG 2 cut(s) 352, 434
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 2 cut(s) 352, 434
MvnI CGCG 3 cut(s) 67, 338, 542
MwoI GCNNNNNNNGC 1 cut(s) 375
NdeII GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 5 cut(s) 105, 323, 324, 356, 431
NmuCI GTSAC 2 cut(s) 610, 652
NruI TCGCGA 1 cut(s) 67
NspV TTCGAA 1 cut(s) 238
PciSI GCTCTTC 2 cut(s) 180, 263
PctI GAATGC 1 cut(s) 601
PfoI TCCNGGA 1 cut(s) 350
PkrI GCNGC 1 cut(s) 344
PleI GAGTC 3 cut(s) 224, 226, 575
PpsI GAGTC 3 cut(s) 224, 226, 575
PpuMI RGGWCCY 2 cut(s) 322, 348
Psp5II RGGWCCY 2 cut(s) 322, 348
Psp6I CCWGG 2 cut(s) 350, 432
PspFI CCCAGC 2 cut(s) 385, 475
PspGI CCWGG 2 cut(s) 350, 432
PspN4I GGNNCC 5 cut(s) 105, 323, 324, 356, 431
PspPI GGNCC 3 cut(s) 322, 348, 552
PspPPI RGGWCCY 2 cut(s) 322, 348
PstNI CAGNNNCTG 1 cut(s) 351
PsuI RGATCY 2 cut(s) 25, 354
RruI TCGCGA 1 cut(s) 67
SapI GCTCTTC 2 cut(s) 180, 263
SatI GCNGC 1 cut(s) 343
Sau3AI GATC 8 cut(s) 25, 33, 68, 96, 246, 354, 390, 645
Sau96I GGNCC 3 cut(s) 322, 348, 552
SchI GAGTC 3 cut(s) 224, 227, 576
ScrFI CCNGG 2 cut(s) 352, 434
SduI GDGCHC 1 cut(s) 432
SetI ASST 9 cut(s) 65, 159, 192, 350, 410, 435, 474, 498, 537
SfaNI GCATC 1 cut(s) 243
SfuI TTCGAA 1 cut(s) 238
SinI GGWCC 2 cut(s) 322, 348
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SsiI CCGC 4 cut(s) 83, 326, 343, 581
SspMI CTAG 2 cut(s) 48, 676
StyD4I CCNGG 2 cut(s) 350, 432
TaaI ACNGT 1 cut(s) 631
TaqI TCGA 6 cut(s) 95, 228, 238, 317, 519, 570
TauI GCSGC 1 cut(s) 345
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 2 cut(s) 610, 652
Tsp45I GTSAC 2 cut(s) 610, 652
TspDTI ATGAA 2 cut(s) 17, 250
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 322, 348
XagI CCTNNNNNAGG 1 cut(s) 316
XspI CTAG 2 cut(s) 48, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.