pycom05g20560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012212
Physical Location & Seq
Forward (+)
23336572 .. 23337467
896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20560.2

Sequence Viewer

Length: 708 bp
ATGACCGTTGGAGCGTGCCAGTTCAGGGGACAAACACGAAATTTCCCCGTCACAGATCTGATGATCTGGAACAATCCTAGCCGTTACAAAAGTTCGCGATCTCGTGAATTCCCGCACACTCTAGGGGTATTTTCGCCAAAGCCCTTACACCCACTTCACCAAATCGCTCAGTCGCCGAGCCACAAGCTTCTCCTGAAGCAATGGCTGAAAGAAGAAGAGCTAAATCTCAACAGGGTCTCTCTCAAAGAATCCCAAATCGACTCGGTTCGAAAGGAAATCACGATTCTTTTCATCATCTTCTTCCTCTTCCACTCCATGGCCCTCACCCTTCTCTTCAGTTCCTCGTCACGGGACCCGCGCGGGTTCGCATGCCACCGGTCCTGGATCCCTTCGCTCTGCTCGCTCTGCTTCTCACTGGGGATCATTTGGGCGATTAGGTATAAAACCGACACGGAGGGCCACCTGGAGAAGTTGTTGGAGAGAGAGAAGGAGGATAAGAATCTTCTGGGAAAGTGCATCGAGGAGCTGAAGAAGAAAGGGTTGGAGTTCGATTTGTTAAAGGAGGTGGACGCGCTGAGGAGGGCCAAGAGCTTGAGAGTCGAAGCGAAGGCAGTAAGGAAGTGGAATGCGAGGGATTTTGTGACACTGTTTTTCTTCACCGTGTCTTGCTTTGTGATCATTGTCACAAGGGTTATTTTGTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

27.36

Weight (kDa)

9.63

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12870
fragaria_vesca FvH4_3g15460
malus_domestica MD01G1075700.v1.1 MD05G1223200.v1.1 MD10G1204600.v1.1 MD12G1131000.v1.1
prunus_persica Prupe.4G137100_v2.0.a1
pyrus_communis pycom05g20560 pycom10g17530
rosa_chinensis RchiOBHm_Chr5g0025961
rosa_laevigata RLG00000032887
rosa_multiflora Rmu_sc0003020.1_g000010
rosa_roxburghii Rroxscaffold_1G00053280
rosa_rugosa Rorug05G0089900
rosa_samantha Rh5AG181700 Rh5BG179800 Rh5CG198200 Rh5DG180700
rosa_wichuraiana Rw5G016530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 316
AccII CGCG 4 cut(s) 97, 358, 360, 572
AciI CCGC 3 cut(s) 113, 356, 360
AclWI GGATC 3 cut(s) 379, 392, 428
AcsI RAATTY 2 cut(s) 40, 107
AcuI CTGAAG 3 cut(s) 215, 319, 548
AfiI CCNNNNNNNGG 3 cut(s) 25, 316, 348
AgeI ACCGGT 1 cut(s) 375
AjnI CCWGG 2 cut(s) 380, 462
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AluBI AGCT 4 cut(s) 187, 220, 526, 591
AluI AGCT 4 cut(s) 187, 220, 526, 591
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 3 cut(s) 379, 392, 428
AoxI GGCC 3 cut(s) 318, 457, 582
ApoI RAATTY 2 cut(s) 40, 107
AsiGI ACCGGT 1 cut(s) 375
AspLEI GCGC 2 cut(s) 360, 574
AspS9I GGNCC 5 cut(s) 319, 352, 378, 457, 582
AsuHPI GGTGA 3 cut(s) 149, 316, 649
AsuII TTCGAA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 352, 378
BamHI GGATCC 1 cut(s) 384
BauI CACGAG 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 575
BceAI ACGGC 1 cut(s) 66
BciT130I CCWGG 2 cut(s) 382, 464
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 3 cut(s) 78, 122, 706
BglII AGATCT 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 382, 464
Bme18I GGWCC 2 cut(s) 352, 378
BmgT120I GGNCC 5 cut(s) 319, 352, 378, 457, 582
BmiI GGNNCC 3 cut(s) 353, 354, 386
BmrFI CCNGG 2 cut(s) 382, 464
BmrI ACTGGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 425
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 575
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 613
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 316, 348
Bse118I RCCGGY 1 cut(s) 375
Bse1I ACTGG 2 cut(s) 19, 420
Bse3DI GCAATG 1 cut(s) 206
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 2 cut(s) 382, 464
BseDI CCNNGG 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 25, 316, 348
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 2 cut(s) 182, 566
BseNI ACTGG 2 cut(s) 19, 420
BseRI GAGGAG 2 cut(s) 536, 592
Bsh1236I CGCG 4 cut(s) 97, 358, 360, 572
BshFI GGCC 3 cut(s) 320, 459, 584
BshTI ACCGGT 1 cut(s) 375
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 42, 365
BslI CCNNNNNNNGG 3 cut(s) 25, 316, 348
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 2 cut(s) 42, 365
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 3 cut(s) 320, 459, 584
Bso31I GGTCTC 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 268
Bsp143I GATC 6 cut(s) 55, 63, 98, 384, 420, 675
Bsp19I CCATGG 1 cut(s) 315
Bsp68I TCGCGA 1 cut(s) 97
BspACI CCGC 3 cut(s) 113, 356, 360
BspANI GGCC 3 cut(s) 320, 459, 584
BspCNI CTCAG 2 cut(s) 181, 567
BspFNI CGCG 4 cut(s) 97, 358, 360, 572
BspLI GGNNCC 3 cut(s) 353, 354, 386
BspPI GGATC 3 cut(s) 379, 392, 428
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 375
BsrI ACTGG 2 cut(s) 19, 420
BssAI RCCGGY 1 cut(s) 375
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 6 cut(s) 55, 63, 98, 384, 420, 675
BssSI CACGAG 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 382, 464
Bst4CI ACNGT 3 cut(s) 7, 648, 661
Bst6I CTCTTC 3 cut(s) 210, 311, 338
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 16, 370, 401
BstDEI CTNAG 2 cut(s) 168, 575
BstDSI CCRYGG 1 cut(s) 315
BstFNI CGCG 4 cut(s) 97, 358, 360, 572
BstHHI GCGC 2 cut(s) 360, 574
BstKTI GATC 6 cut(s) 58, 66, 101, 387, 423, 678
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 6 cut(s) 55, 63, 98, 384, 420, 675
BstMWI GCNNNNNNNGC 2 cut(s) 400, 405
BstNI CCWGG 2 cut(s) 382, 464
BstNSI RCATGY 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 380, 462
BstUI CGCG 4 cut(s) 97, 358, 360, 572
BstX2I RGATCY 2 cut(s) 55, 384
BstYI RGATCY 2 cut(s) 55, 384
BsuRI GGCC 3 cut(s) 320, 459, 584
BtgI CCRYGG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 413, 644
BtuMI TCGCGA 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 16, 370, 401
CfoI GCGC 2 cut(s) 360, 574
Cfr10I RCCGGY 1 cut(s) 375
Cfr13I GGNCC 5 cut(s) 319, 352, 378, 457, 582
CseI GACGC 1 cut(s) 578
CspAI ACCGGT 1 cut(s) 375
CviAII CATG 2 cut(s) 316, 369
DdeI CTNAG 2 cut(s) 168, 575
DpnI GATC 6 cut(s) 57, 65, 100, 386, 422, 677
DpnII GATC 6 cut(s) 55, 63, 98, 384, 420, 675
Eam1104I CTCTTC 3 cut(s) 210, 311, 338
EarI CTCTTC 3 cut(s) 210, 311, 338
Eco130I CCWWGG 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 352, 378
Eco57I CTGAAG 3 cut(s) 215, 319, 548
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 380, 462
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 319, 372
FaiI YATR 3 cut(s) 317, 370, 441
FaqI GGGAC 2 cut(s) 42, 365
FatI CATG 2 cut(s) 315, 368
FauI CCCGC 3 cut(s) 120, 353, 363
FbaI TGATCA 1 cut(s) 675
FspBI CTAG 3 cut(s) 78, 122, 706
GlaI GCGC 2 cut(s) 359, 573
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 320, 459, 584
HapII CCGG 1 cut(s) 376
HgaI GACGC 1 cut(s) 578
HhaI GCGC 2 cut(s) 360, 574
Hin1II CATG 2 cut(s) 319, 372
Hin6I GCGC 2 cut(s) 358, 572
HinP1I GCGC 2 cut(s) 358, 572
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 5 cut(s) 248, 260, 283, 499, 597
HpaII CCGG 1 cut(s) 376
HphI GGTGA 3 cut(s) 149, 316, 649
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 5 cut(s) 67, 96, 104, 193, 280
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 4 cut(s) 338, 399, 481, 601
HpyCH4III ACNGT 3 cut(s) 7, 648, 661
HpyCH4V TGCA 1 cut(s) 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 400, 405
HpyF3I CTNAG 2 cut(s) 168, 575
Hsp92II CATG 2 cut(s) 319, 372
HspAI GCGC 2 cut(s) 358, 572
KflI GGGWCCC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 6 cut(s) 55, 63, 98, 384, 420, 675
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 11, 523
LweI GCATC 1 cut(s) 525
MaeI CTAG 3 cut(s) 78, 122, 706
MaeIII GTNAC 6 cut(s) 49, 83, 345, 640, 682, 701
MalI GATC 6 cut(s) 57, 65, 100, 386, 422, 677
MboI GATC 6 cut(s) 55, 63, 98, 384, 420, 675
MflI RGATCY 2 cut(s) 55, 384
MluCI AATT 2 cut(s) 40, 107
MlyI GAGTC 2 cut(s) 254, 606
MmeI TCCRAC 2 cut(s) 456, 522
MseI TTAA 1 cut(s) 557
MspI CCGG 1 cut(s) 376
MspR9I CCNGG 2 cut(s) 382, 464
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 2 cut(s) 382, 464
MvnI CGCG 4 cut(s) 97, 358, 360, 572
MwoI GCNNNNNNNGC 2 cut(s) 400, 405
NcoI CCATGG 1 cut(s) 315
NdeII GATC 6 cut(s) 55, 63, 98, 384, 420, 675
NlaIII CATG 2 cut(s) 319, 372
NlaIV GGNNCC 3 cut(s) 353, 354, 386
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 4 cut(s) 49, 345, 640, 682
NruI TCGCGA 1 cut(s) 97
NspI RCATGY 1 cut(s) 372
NspV TTCGAA 1 cut(s) 268
PaeI GCATGC 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 210
PctI GAATGC 1 cut(s) 631
PfeI GAWTC 3 cut(s) 248, 283, 499
PflMI CCANNNNNTGG 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 380
PinAI ACCGGT 1 cut(s) 375
PleI GAGTC 2 cut(s) 254, 605
PpsI GAGTC 2 cut(s) 254, 605
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 380, 462
PspGI CCWGG 2 cut(s) 380, 462
PspN4I GGNNCC 3 cut(s) 353, 354, 386
PspPI GGNCC 5 cut(s) 319, 352, 378, 457, 582
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 2 cut(s) 55, 384
RruI TCGCGA 1 cut(s) 97
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 557
Sau3AI GATC 6 cut(s) 55, 63, 98, 384, 420, 675
Sau96I GGNCC 5 cut(s) 319, 352, 378, 457, 582
SchI GAGTC 2 cut(s) 254, 606
ScrFI CCNGG 2 cut(s) 382, 464
SetI ASST 7 cut(s) 189, 222, 440, 465, 528, 567, 593
SfaNI GCATC 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 2 cut(s) 352, 378
SmlI CTYRAG 1 cut(s) 592
SmoI CTYRAG 1 cut(s) 592
SphI GCATGC 1 cut(s) 372
Sse9I AATT 2 cut(s) 40, 107
SsiI CCGC 3 cut(s) 113, 356, 360
SspMI CTAG 3 cut(s) 78, 122, 706
StyD4I CCNGG 2 cut(s) 380, 462
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 7, 648, 661
TaqI TCGA 5 cut(s) 258, 268, 519, 549, 600
TasI AATT 2 cut(s) 40, 107
TfiI GAWTC 3 cut(s) 248, 283, 499
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 2 cut(s) 420, 651
TseFI GTSAC 4 cut(s) 49, 345, 640, 682
Tsp45I GTSAC 4 cut(s) 49, 345, 640, 682
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 2 cut(s) 420, 651
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 352, 378
XapI RAATTY 2 cut(s) 40, 107
XceI RCATGY 1 cut(s) 372
XspI CTAG 3 cut(s) 78, 122, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.