MD10G1204600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012212
Physical Location & Seq
Forward (+)
30357258 .. 30357866
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1204600.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCCGACGAGAGCACAAACAAATCGATCATGGAGCCGAAGTTCTCTCACCCACTTCACCAAATCTCTCAGTCTCCGAGCCACAAGCTTCTCCTGAAGCAATGGCTGAAAGAAGAAGAGCTAATCCTCAACAGGGTCTCTCTCAAGGAGTCCCAAATCGACTCGGTTCGAAAGGAGATCACGATGCTCTTCATCTTCTTCTTCCTCTTCCACTCCACGGCTCTCACCCTTCTCTTCAGTTCATCGTCTCGGGACCCGAACGGGTCCGCGTGCCGCAGGTCCTGGATCCCATCGCTCTGCTCTCTCTGCTTCTCGCTGGGGATCATTTGGGCGATAAGGTATAAAAGCGACACGGAGGGGCACCTGGAGAAGTTGTTGGAGAGAGAAAAGGAGGATAAGAACCTTCTGGGAAAATGCATTGAGGAGCTGAAGAAGAAGGGGTTGGAGTTCGATTTGTTGAAGGAGGTCGACGCGCTGAGGAGGGCCAAGAGTTTGAGAGTCGAAGCGAAGGCGGTGAGGAAGTGGAATGCGAGGGATTTTGTGACTCTGTTTTTCTTCACTGTGTCTTGCTTTGTGATCATTGTCACCAGAGTTATTTTGTGTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

23.46

Weight (kDa)

9.15

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12870
fragaria_vesca FvH4_3g15460
malus_domestica MD01G1075700.v1.1 MD05G1223200.v1.1 MD10G1204600.v1.1 MD12G1131000.v1.1
prunus_persica Prupe.4G137100_v2.0.a1
pyrus_communis pycom05g20560 pycom10g17530
rosa_chinensis RchiOBHm_Chr5g0025961
rosa_laevigata RLG00000032887
rosa_multiflora Rmu_sc0003020.1_g000010
rosa_roxburghii Rroxscaffold_1G00053280
rosa_rugosa Rorug05G0089900
rosa_samantha Rh5AG181700 Rh5BG179800 Rh5CG198200 Rh5DG180700
rosa_wichuraiana Rw5G016530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 360
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 269, 473
AciI CCGC 3 cut(s) 267, 274, 512
AclWI GGATC 3 cut(s) 280, 293, 329
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 116, 220, 449
AfiI CCNNNNNNNGG 2 cut(s) 133, 217
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 2 cut(s) 281, 363
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
AluBI AGCT 3 cut(s) 88, 121, 427
AluI AGCT 3 cut(s) 88, 121, 427
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 78, 142, 252
AlwI GGATC 3 cut(s) 280, 293, 329
AlwNI CAGNNNCTG 1 cut(s) 282
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 2 cut(s) 3, 483
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 4 cut(s) 253, 264, 279, 483
AsuHPI GGTGA 5 cut(s) 41, 50, 217, 526, 577
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 3 cut(s) 253, 264, 279
BaeGI GKGCMC 1 cut(s) 363
BamHI GGATCC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 298
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 2 cut(s) 283, 365
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 3 cut(s) 78, 142, 252
BfaI CTAG 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 267
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme1390I CCNGG 2 cut(s) 283, 365
Bme18I GGWCC 3 cut(s) 253, 264, 279
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 4 cut(s) 253, 264, 279, 483
BmiI GGNNCC 6 cut(s) 36, 254, 255, 265, 287, 362
BmrFI CCNGG 2 cut(s) 283, 365
BmsI GCATC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 476
Bpu14I TTCGAA 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 128
Bsa29I ATCGAT 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 216
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 217
Bse3DI GCAATG 1 cut(s) 107
BseBI CCWGG 2 cut(s) 283, 365
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 216
BseLI CCNNNNNNNGG 2 cut(s) 133, 217
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 2 cut(s) 83, 467
BseRI GAGGAG 2 cut(s) 437, 493
BseSI GKGCMC 1 cut(s) 363
BseYI CCCAGC 1 cut(s) 316
Bsh1236I CGCG 2 cut(s) 269, 473
BshFI GGCC 2 cut(s) 5, 485
BshNI GGYRCC 1 cut(s) 360
BshVI ATCGAT 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 249
BslFI GGGAC 2 cut(s) 136, 266
BslI CCNNNNNNNGG 2 cut(s) 133, 217
BsmAI GTCTC 3 cut(s) 78, 142, 252
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 136, 266
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 5, 485
Bso31I GGTCTC 1 cut(s) 142
BsoBI CYCGRG 1 cut(s) 249
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 17, 363
Bsp143I GATC 5 cut(s) 27, 177, 285, 321, 576
BspACI CCGC 3 cut(s) 267, 274, 512
BspANI GGCC 2 cut(s) 5, 485
BspCNI CTCAG 2 cut(s) 82, 468
BspDI ATCGAT 1 cut(s) 26
BspFNI CGCG 2 cut(s) 269, 473
BspLI GGNNCC 6 cut(s) 36, 254, 255, 265, 287, 362
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 3 cut(s) 280, 293, 329
BspQI GCTCTTC 2 cut(s) 111, 194
BspT104I TTCGAA 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 360
BspTNI GGTCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 5 cut(s) 27, 177, 285, 321, 576
Bst2UI CCWGG 2 cut(s) 283, 365
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 4 cut(s) 111, 194, 212, 239
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 2 cut(s) 69, 476
BstDSI CCRYGG 1 cut(s) 216
BstFNI CGCG 2 cut(s) 269, 473
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 5 cut(s) 30, 180, 288, 324, 579
BstMAI GTCTC 3 cut(s) 78, 142, 252
BstMBI GATC 5 cut(s) 27, 177, 285, 321, 576
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 2 cut(s) 283, 365
BstSCI CCNGG 2 cut(s) 281, 363
BstSLI GKGCMC 1 cut(s) 363
BstUI CGCG 2 cut(s) 269, 473
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 5, 485
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 558
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 271
CaiI CAGNNNCTG 1 cut(s) 282
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 4 cut(s) 253, 264, 279, 483
ClaI ATCGAT 1 cut(s) 26
CseI GACGC 1 cut(s) 479
CviAII CATG 1 cut(s) 31
CviJI RGCY 9 cut(s) 5, 37, 81, 88, 106, 121, 221, 427, 485
CviKI_1 RGCY 9 cut(s) 5, 37, 81, 88, 106, 121, 221, 427, 485
DdeI CTNAG 2 cut(s) 69, 476
DpnI GATC 5 cut(s) 29, 179, 287, 323, 578
DpnII GATC 5 cut(s) 27, 177, 285, 321, 576
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 111, 194, 212, 239
EarI CTCTTC 4 cut(s) 111, 194, 212, 239
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 253, 264, 279
Eco57I CTGAAG 3 cut(s) 116, 220, 449
Eco88I CYCGRG 1 cut(s) 249
EcoO109I RGGNCCY 2 cut(s) 253, 279
EcoRII CCWGG 2 cut(s) 281, 363
EcoT22I ATGCAT 1 cut(s) 419
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 1 cut(s) 34
FaiI YATR 2 cut(s) 32, 342
FaqI GGGAC 2 cut(s) 136, 266
FatI CATG 1 cut(s) 30
FbaI TGATCA 1 cut(s) 576
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 274
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 1 cut(s) 607
GlaI GCGC 1 cut(s) 474
GluI GCNGC 1 cut(s) 274
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 2 cut(s) 5, 485
HgaI GACGC 1 cut(s) 479
HhaI GCGC 1 cut(s) 475
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 4 cut(s) 149, 161, 498, 544
HphI GGTGA 5 cut(s) 41, 50, 217, 526, 577
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 3 cut(s) 94, 181, 251
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 2 cut(s) 11, 473
HpyAV CCTTC 5 cut(s) 239, 413, 430, 454, 502
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4V TGCA 1 cut(s) 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 2 cut(s) 69, 476
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 1 cut(s) 473
KflI GGGWCCC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 5 cut(s) 27, 177, 285, 321, 576
LguI GCTCTTC 2 cut(s) 111, 194
LmnI GCTCC 2 cut(s) 34, 424
LweI GCATC 1 cut(s) 174
MaeI CTAG 1 cut(s) 607
MaeIII GTNAC 3 cut(s) 541, 583, 602
MalI GATC 5 cut(s) 29, 179, 287, 323, 578
MboI GATC 5 cut(s) 27, 177, 285, 321, 576
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 2 cut(s) 17, 363
MlyI GAGTC 4 cut(s) 155, 158, 507, 538
MmeI TCCRAC 2 cut(s) 357, 423
Mph1103I ATGCAT 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 283, 365
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 2 cut(s) 283, 365
MvnI CGCG 2 cut(s) 269, 473
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeII GATC 5 cut(s) 27, 177, 285, 321, 576
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 6 cut(s) 36, 254, 255, 265, 287, 362
NmuCI GTSAC 2 cut(s) 541, 583
NsiI ATGCAT 1 cut(s) 419
NspV TTCGAA 1 cut(s) 169
PciSI GCTCTTC 2 cut(s) 111, 194
PctI GAATGC 1 cut(s) 532
PfoI TCCNGGA 1 cut(s) 281
PkrI GCNGC 1 cut(s) 275
PleI GAGTC 4 cut(s) 155, 157, 506, 538
PpsI GAGTC 4 cut(s) 155, 157, 506, 538
PpuMI RGGWCCY 2 cut(s) 253, 279
Psp5II RGGWCCY 2 cut(s) 253, 279
Psp6I CCWGG 2 cut(s) 281, 363
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 2 cut(s) 281, 363
PspN4I GGNNCC 6 cut(s) 36, 254, 255, 265, 287, 362
PspPI GGNCC 4 cut(s) 253, 264, 279, 483
PspPPI RGGWCCY 2 cut(s) 253, 279
PstNI CAGNNNCTG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 285
SalI GTCGAC 1 cut(s) 467
SapI GCTCTTC 2 cut(s) 111, 194
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 5 cut(s) 27, 177, 285, 321, 576
Sau96I GGNCC 4 cut(s) 253, 264, 279, 483
SchI GAGTC 4 cut(s) 155, 158, 507, 538
ScrFI CCNGG 2 cut(s) 283, 365
SduI GDGCHC 2 cut(s) 17, 363
SetI ASST 8 cut(s) 90, 123, 281, 341, 366, 405, 429, 468
SfaNI GCATC 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 3 cut(s) 253, 264, 279
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SsiI CCGC 3 cut(s) 267, 274, 512
SspMI CTAG 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 281, 363
TaaI ACNGT 1 cut(s) 562
TaqI TCGA 6 cut(s) 26, 159, 169, 450, 468, 501
TauI GCSGC 1 cut(s) 276
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 2 cut(s) 541, 583
Tsp45I GTSAC 2 cut(s) 541, 583
TspDTI ATGAA 2 cut(s) 181, 231
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 1 cut(s) 565
VpaK11BI GGWCC 3 cut(s) 253, 264, 279
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 1 cut(s) 607
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.