Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006161

Physical Location & Seq

Genomic Coordinates Forward (+)

37075707 .. 37090586

Gene Structure 14880 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1239900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3774 bp

ATGATGCAGCAGCATCAACCTCCGCCAGTCCCACTGCAATCGCAGACCGCCGGAACCCCAGGCGGAGTGCAGGTCCGCTGCGCCGGCTGCGCCAAGATCCTGACTTTGGACGCGGGGGTCACCGAATTCGCCTGCGACGACTGCCAATTGCCCCAGATGCTGCCGCCCGAGCTCATGACCCGAGCCCAGGCCCATGCCCAAGCCCACCCTGGGCCCAACAAGGCCCGCACTCCGCCTCCACTGCCGCCTCCACCGCCCCATGTTGCGCCTCACGGCGTTGATCCCTCGAAGATCCAGATCCCCTGCGCCAACTGCAAGGCCATCCTCAACGTGCCTCATGGCCTCGCGAGGTTCCGGTGCCCTCAGTGCCAAGTTGACCTCGCCGTCGATGTGTCAAAGCTCCAGCAGTTCTTCCCTCCCCGTCTGCCCCTGCCGCCTCCGCCCGAGGAGGTCAATGAGGTTGCCATTGAAGTGGAGCGAGAAGAGGATGAAGGTGGCATGGCAGGAGAAACATTTACGGACTATCGCCCTCCAAAACTATCCATTGGCCCACCTCATCCAGATCCTGTTGTGGAAACATCGTCCTTGTCTGCTGTGCAGCCACCTGAGCCCACTTATGATCTGAAAATCAAGGATGATTTGGAAAATAAAAAGGCTTTGTCATGTTTACAGATTGAAACATTGGTCTATGCTTGTCAGAGACACCTGCAACATCTTCCAAGTGGTGAGAGGGCGGGATTCTTTGTCGGGGATGGAGCTGGTGTGGGGAAAGGTCGAACAATAGCAGGGTTAATATGGGAGAATTGGCACCGTGGGATGAAGAAAGCAGTGTGGGTTTCTGTTGGTTCAGACTTGAAGTTTGATGCAAGAAGAGACTTAGATGATGTATGTGCAACTTGCATTGAAGTACATGCCTTGAACAAGCTGCCTTATTCCAAACTTGATTCAAAGTCTGTTGGCGTCAAGGAGGGCGTTATTTTTCTGACATACAGCAGCCTCATTGCATCCTCTGAGAAAGGTCGTTCACGTATGGGGCAGCTTGTGCAGTGGTGTGGACCAGGATTTAATGGTCTCATCATATTTGATGAGTGCCACAAGGCCAAAAATCTGGTGCCTGAATCTGGAAGTCAGCCAACACGTACTGGTGAAGCAGTTCTTGAAATTCAGGCTAAGCTTCCTGAAGCTCGTGTTATTTATTGCTCAGCAACTGGTGCATCTGAACCACGAAATTTGGGTTACATGGTACGGCTTGGTCTTTGGGGGCCTGGAACATCTTTTGCTGATTTCCGTGAATTTCTAGGTGCTATTGAGAAGGGAGGTGTTGGAGCACTGGAACTTGTTGCCATGGATATGAAAGCAAGGGGGATGTATGTATGTCGAACTCTTAGCTACAATGGGGTAGAGTTTGAAGTTGTTGAAGCACCGCTAGAAGCTGAAATGATGGACATGTACAAAAAAGCAGCAGAATTTTGGACAGAGCTGCGACAGGATATACTAACAGCATCTCAATTTCTCACTAATGAAAAGCCAATTTCTAGTCAAGTGTGGCGATTATATTGGGCTAGCCATCAGCGTTTCTTTAGACATATGTGCATGTCAGCTAAGGTGCCGGCTGCTGTAAGACTGGCTAAGCAAGCATTGATGGATGGCAAGTGTGTGGTTATTGGCCTTCAGAGTACTGGAGAGGCGCGAACTGAGGAAGCTGTGACCAAATATGGTCTTGAACTTGATGATTTTATTTCCGGACCTCGAGAACTTTTACTGAAATTTGTTGAGGAAAATTATCCTTTGCCTGAAAAGCCTGAACCTCTCGAAGGAGAAGATAATGTGAAGGAGCTGCAGCGGAAGAGGCACTCAGCAACTCCTGGTGTATCAATGAAAGGGAGAGTGAGGAAAGTTGCAAAATGGAAACCTGCAAGTGATGAAGATTCTGAAACTGATTCTGCTCAGGAATCTACTGAATCTGACGACGAGTTTCAAATATGTGAAATTTGCCAATCTGAGGAGGAAAGAAAGAAACTGCTTCATTGTTCCTGTTGTGGCCAACTGGTCCATGCTGCATGTCTTATCCCACCTGTGACAGATGAAGTATCAGTAGATTGGTCATGTCATTCATGCAAGGAAAAAACAGAGGAATTTTTGAAAAGAAGACAAGAATATATAGCTGTACTTACGAAGATGTATGAAGAAGCTTTGGCTCGTAAGTTAAACATTTTGGAGATAGTTCGTTCTTTGGATCTTCCGAACAATCCTTTGGATGATATCATTGATCAGCTTGGAGGCCCTGATAAAGTAGCAGAAATGACTGGAAGGAGAGGTATGCTTGTGAGGGCATCTGGTGGAAAAGGTGTTACCTATCAGGCACGGAACACGAAGGAAATTTCCATGGAAATGGTAAACATGCATGAGAAGCAGCTTTTCATGGAAGGCAAGAAGTTGGTTGCCATCATTTCTGAAGCTGGATCTGCTGGCGTGTCTTTGCAGGCAGATAGAAGAGCTACAAATCAGAGACGAAGGGTGCATCTTACTCTGGAACTTCCTTGGAGTGCTGATCGTGCGATTCAACAATTTGGAAGAACTCACCGGTCAAATCAAGCCTCCGCTCCTGAATACAGGCTTCTCTTTACTAACCTTGGTGGAGAGCGTCGATTTGCATCCATTGTAGCAAAGAGATTAGAATCTCTAGGGGCACTAACACAGGGTGACCGCAGGGCAGGGCCATCATTGAGTGCCTATAACTACGATAGTGCTTATGGAAAGAAGGCCCTGATGTTGATGTATAGAGGAATCATGGAGCAGGATTCTCTGCCTGTTGTACCGCCAGGATGTTCATCAGAAAAACCGGAAACAATCCAAGATTTTATCGAGAGGGCTAAAGCTGCTCTTGTTTTAGTAGGAATAATTAGGGATGCTCATGGGAAAGATCATGGCAGGCTCACTGGCCGTATAATTGAATCAGATATGCATGATGTTGGACGTTTCCTCAATCGGATTTTAGGAGTACCGCCCGATATACAGAATAGGCTGTTTGAGTGTTTCGTTAGCATTTTGGATCTTATTGTCCATAATGCACGCATTGAAGGGAATCTTGACTCTGGGATTGTTGATATGAAAGCCAATGTTATAGAGCTACAAGGAACTCCAAAGACTGTTTATGTTGATCAAATGTCTGGAGCGTCAACAGTGCTTTTTACATTTACCCTGGATCGTGGGGTTACGTGGGAGTCTGCTAGTGCCATGCTTGAAGAGAAACAAAAGGATGGACTTGGTTCTGCCAATGATGGGTTCTATGAATCTAGGAGGGAATGGTTGGGGAGACGTCATGTCATATTAGCCTTTGAGAGTTCTACTTCTGGATTGTATAAGATAGTCCGTCCTGCGGTTGGAGAGTCAGTGAGAGAAATGCCTCTGTCAGAGCTAAAAAGCAAATACAGAAAAACTTCAACGGTGGAGAAGGCTCGCGGTGGTTGGGAAGATGAATATGAAGTTTCGTCCAAACAGTGCATGCATGGACGCAATTGTAAGCTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGCCTCATTCTTCCTGTCTGGGGCACTATTGAGAAGGCTCTTTCGAAGCAGCAACGGCAAAGCCACAAGCGGCTACGTGTTGTGCGTATAGAAACCACGACGGATAACCGCAGAATCGTTGGTCTATCTGTCCCAAATGCGGCAGTGGAATCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATCGACGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1258

Amino Acids

139.46

Weight (kDa)

6.39

Isoelectric Point (pI)

51.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_FGT1_1	PF23547	23 - 57	2.8e-18	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1	PF23547	98 - 127	3.6e-10	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2	PF23548	98 - 131	5.4e-17	FORGETTER1 second zinc ribbon domain
AAA_34	PF13872	185 - 485	4.4e-133	P-loop containing NTP hydrolase pore-1
PHD	PF00628	662 - 706	5.7e-06	PHD-finger
Helicase_C_4	PF13871	750 - 1036	5.7e-105	C-terminal domain on Strawberry notch homologue
SBNO	PF25373	1073 - 1185	5.8e-19	SBNO alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006161)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79350
fragaria_vesca	FvH4_3g13920 FvH4_3g13920 FvH4_3g13920 FvH4_3g13920
malus_domestica	MD05G1239900.v1.1 MD10G1218000.v1.1
prunus_persica	Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1
pyrus_communis	pycom05g21600 pycom10g18380
rosa_chinensis	RchiOBHm_Chr5g0023151
rosa_laevigata	RLG00000032703
rosa_multiflora	Rmu_co8366291.1_g000001 Rmu_sc0007300.1_g000019 Rmu_sc0015109.1_g000002 Rmu_sc0033377.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056190
rosa_rugosa	Rorug05G0077600
rosa_samantha	Rh5AG163600 Rh5BG163000 Rh5CG177800 Rh5DG164200
rosa_wichuraiana	Rw5G014760 Rw5G015070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	714
AasI	GACNNNNNNGTC	2 cut(s)	116, 383
AatII	GACGTC	1 cut(s)	3322
Acc36I	ACCTGC	3 cut(s)	61, 714, 1921
AccB1I	GGYRCC	4 cut(s)	357, 807, 1111, 1606
AccBSI	CCGCTC	1 cut(s)	2603
AccII	CGCG	4 cut(s)	113, 347, 1690, 3462
AccIII	TCCGGA	1 cut(s)	1742
AclWI	GGATC	9 cut(s)	91, 275, 286, 292, 557, 2244, 2470, 3060, 3213
AcoI	YGGCCR	2 cut(s)	2041, 2941
AcsI	RAATTY	9 cut(s)	125, 1161, 1228, 1292, 1466, 1766, 1989, 2135, 2379
AcuI	CTGAAG	3 cut(s)	1200, 1655, 2475
AcyI	GRCGYC	2 cut(s)	960, 3319
AdeI	CACNNNGTG	1 cut(s)	2702
AflIII	ACRYGT	3 cut(s)	1136, 1446, 3655
AgeI	ACCGGT	1 cut(s)	2583
AhdI	GACNNNNNGTC	1 cut(s)	3323
AjnI	CCWGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
AjuI	GAANNNNNNNTTGG	4 cut(s)	2237, 2269, 3269, 3301
Alw21I	GWGCWC	2 cut(s)	174, 1330
Alw26I	GTCTC	5 cut(s)	694, 867, 1076, 2503, 3310
AlwI	GGATC	9 cut(s)	91, 275, 286, 292, 557, 2244, 2470, 3060, 3213
AlwNI	CAGNNNCTG	3 cut(s)	160, 566, 1208
Ama87I	CYCGRG	4 cut(s)	167, 180, 443, 1749
Aor13HI	TCCGGA	1 cut(s)	1742
ApaI	GGGCCC	1 cut(s)	216
ApoI	RAATTY	9 cut(s)	125, 1161, 1228, 1292, 1466, 1766, 1989, 2135, 2379
ArsI	GACNNNNNNTTYG	2 cut(s)	842, 874
AsiGI	ACCGGT	1 cut(s)	2583
Asp700I	GAANNNNTTC	2 cut(s)	1152, 3439
AspLEI	GCGC	5 cut(s)	83, 92, 268, 308, 1690
AsuHPI	GGTGA	5 cut(s)	112, 737, 1157, 2573, 2714
AsuII	TTCGAA	1 cut(s)	3623
AsuNHI	GCTAGC	1 cut(s)	1562
AvaI	CYCGRG	4 cut(s)	167, 180, 443, 1749
AvaII	GGWCC	4 cut(s)	73, 1055, 1745, 2050
BaeGI	GKGCMC	4 cut(s)	216, 362, 2692, 3605
BalI	TGGCCA	1 cut(s)	2043
BanI	GGYRCC	4 cut(s)	357, 807, 1111, 1606
BanII	GRGCYC	4 cut(s)	174, 187, 216, 612
BauI	CACGAG	1 cut(s)	1185
BbsI	GAAGAC	2 cut(s)	2155, 3559
Bbv12I	GWGCWC	2 cut(s)	174, 1330
BceAI	ACGGC	5 cut(s)	289, 368, 1262, 2928, 3650
BciT130I	CCWGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
BclI	TGATCA	2 cut(s)	2269, 3160
BcoDI	GTCTC	5 cut(s)	694, 867, 1076, 2503, 3310
BfaI	CTAG	7 cut(s)	1298, 1427, 1536, 1563, 2684, 3231, 3297
BfmI	CTRYAG	2 cut(s)	1838, 3557
BfuAI	ACCTGC	3 cut(s)	61, 714, 1921
BlpI	GCTNAGC	3 cut(s)	1170, 1201, 1629
BmcAI	AGTACT	1 cut(s)	1678
Bme1390I	CCNGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
Bme18I	GGWCC	4 cut(s)	73, 1055, 1745, 2050
BmeRI	GACNNNNNGTC	1 cut(s)	3323
BmeT110I	CYCGRG	4 cut(s)	167, 180, 443, 1749
BmiI	GGNNCC	8 cut(s)	55, 214, 353, 359, 809, 1113, 1263, 1608
BmrFI	CCNGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
BmtI	GCTAGC	1 cut(s)	1566
BpiI	GAAGAC	2 cut(s)	2155, 3559
BpmI	CTGGAG	3 cut(s)	386, 1701, 3192
Bpu10I	CCTNAGC	3 cut(s)	606, 1602, 1947
Bpu1102I	GCTNAGC	3 cut(s)	1170, 1201, 1629
Bpu14I	TTCGAA	1 cut(s)	3623
BsaAI	YACGTR	4 cut(s)	1028, 1139, 3219, 3656
BsaBI	GATNNNNATC	4 cut(s)	296, 2653, 2677, 2830
BsaHI	GRCGYC	2 cut(s)	960, 3319
BsaI	GGTCTC	1 cut(s)	1076
BsaWI	WCCGGW	4 cut(s)	354, 1742, 2583, 2842
BsaXI	ACNNNNNCTCC	8 cut(s)	57, 87, 220, 250, 747, 777, 3378, 3408
Bse118I	RCCGGY	3 cut(s)	83, 1609, 2583
Bse1I	ACTGG	9 cut(s)	26, 1147, 1213, 1335, 1629, 1684, 2052, 2311, 2944
Bse3DI	GCAATG	1 cut(s)	999
Bse8I	GATNNNNATC	4 cut(s)	296, 2653, 2677, 2830
BseAI	TCCGGA	1 cut(s)	1742
BseBI	CCWGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
BseJI	GATNNNNATC	4 cut(s)	296, 2653, 2677, 2830
BseMI	GCAATG	1 cut(s)	999
BseMII	CTCAG	8 cut(s)	377, 597, 1002, 1215, 1686, 1869, 1961, 1992
BseNI	ACTGG	9 cut(s)	26, 1147, 1213, 1335, 1629, 1684, 2052, 2311, 2944
BseRI	GAGGAG	2 cut(s)	461, 2018
BseSI	GKGCMC	4 cut(s)	216, 362, 2692, 3605
BsgI	GTGCAG	3 cut(s)	89, 617, 1064
Bsh1236I	CGCG	4 cut(s)	113, 347, 1690, 3462
BshNI	GGYRCC	4 cut(s)	357, 807, 1111, 1606
BshTI	ACCGGT	1 cut(s)	2583
BsiHKAI	GWGCWC	2 cut(s)	174, 1330
BsiHKCI	CYCGRG	4 cut(s)	167, 180, 443, 1749
BsiSI	CCGG	7 cut(s)	51, 84, 355, 1610, 1743, 2584, 2843
BslFI	GGGAC	2 cut(s)	14, 3695
BsmAI	GTCTC	5 cut(s)	694, 867, 1076, 2503, 3310
BsmBI	CGTCTC	2 cut(s)	2503, 3310
BsmFI	GGGAC	2 cut(s)	14, 3695
Bso31I	GGTCTC	1 cut(s)	1076
BsoBI	CYCGRG	4 cut(s)	167, 180, 443, 1749
Bsp119I	TTCGAA	1 cut(s)	3623
Bsp120I	GGGCCC	1 cut(s)	212
Bsp1286I	GDGCHC	8 cut(s)	174, 187, 216, 362, 612, 1330, 2692, 3605
Bsp13I	TCCGGA	1 cut(s)	1742
Bsp1407I	TGTACA	1 cut(s)	1449
Bsp1720I	GCTNAGC	3 cut(s)	1170, 1201, 1629
Bsp19I	CCATGG	2 cut(s)	1344, 2385
Bsp68I	TCGCGA	1 cut(s)	347
BspCNI	CTCAG	8 cut(s)	376, 598, 1003, 1214, 1687, 1868, 1960, 1993
BspEI	TCCGGA	1 cut(s)	1742
BspFNI	CGCG	4 cut(s)	113, 347, 1690, 3462
BspHI	TCATGA	1 cut(s)	174
BspLI	GGNNCC	8 cut(s)	55, 214, 353, 359, 809, 1113, 1263, 1608
BspMAI	CTGCAG	2 cut(s)	1842, 3561
BspMI	ACCTGC	3 cut(s)	61, 714, 1921
BspOI	GCTAGC	1 cut(s)	1566
BspPI	GGATC	9 cut(s)	91, 275, 286, 292, 557, 2244, 2470, 3060, 3213
BspQI	GCTCTTC	1 cut(s)	2488
BspT104I	TTCGAA	1 cut(s)	3623
BspT107I	GGYRCC	4 cut(s)	357, 807, 1111, 1606
BspTNI	GGTCTC	1 cut(s)	1076
BsrBI	CCGCTC	1 cut(s)	2603
BsrDI	GCAATG	1 cut(s)	999
BsrFI	RCCGGY	3 cut(s)	83, 1609, 2583
BsrGI	TGTACA	1 cut(s)	1449
BsrI	ACTGG	9 cut(s)	26, 1147, 1213, 1335, 1629, 1684, 2052, 2311, 2944
BssAI	RCCGGY	3 cut(s)	83, 1609, 2583
BssNI	GRCGYC	2 cut(s)	960, 3319
BssSI	CACGAG	1 cut(s)	1185
BssT1I	CCWWGG	4 cut(s)	1344, 2385, 2540, 2632
Bst2BI	CACGAG	1 cut(s)	1185
Bst2UI	CCWGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
Bst4CI	ACNGT	6 cut(s)	812, 3151, 3184, 3448, 3501, 3544
Bst6I	CTCTTC	5 cut(s)	477, 865, 1841, 2488, 3240
BstACI	GRCGYC	2 cut(s)	960, 3319
BstAPI	GCANNNNNTGC	2 cut(s)	1042, 1211
BstAUI	TGTACA	1 cut(s)	1449
BstBAI	YACGTR	4 cut(s)	1028, 1139, 3219, 3656
BstBI	TTCGAA	1 cut(s)	3623
BstDSI	CCRYGG	3 cut(s)	811, 1344, 2385
BstEII	GGTNACC	2 cut(s)	118, 2702
BstENI	CCTNNNNNAGG	1 cut(s)	1812
BstFNI	CGCG	4 cut(s)	113, 347, 1690, 3462
BstHHI	GCGC	5 cut(s)	83, 92, 268, 308, 1690
BstMAI	GTCTC	5 cut(s)	694, 867, 1076, 2503, 3310
BstNI	CCWGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
BstNSI	RCATGY	6 cut(s)	914, 1450, 1597, 2064, 2404, 3508
BstPI	GGTNACC	2 cut(s)	118, 2702
BstSCI	CCNGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
BstSFI	CTRYAG	2 cut(s)	1838, 3557
BstSLI	GKGCMC	4 cut(s)	216, 362, 2692, 3605
BstUI	CGCG	4 cut(s)	113, 347, 1690, 3462
BstV2I	GAAGAC	2 cut(s)	2155, 3559
BstX2I	RGATCY	7 cut(s)	96, 291, 297, 562, 2236, 2462, 3052
BstXI	CCANNNNNNTGG	3 cut(s)	472, 1108, 2392
BstYI	RGATCY	7 cut(s)	96, 291, 297, 562, 2236, 2462, 3052
BtgI	CCRYGG	3 cut(s)	811, 1344, 2385
BtsI	GCAGTG	5 cut(s)	32, 239, 834, 1052, 3729
BtuMI	TCGCGA	1 cut(s)	347
BveI	ACCTGC	3 cut(s)	61, 714, 1921
CaiI	CAGNNNCTG	3 cut(s)	160, 566, 1208
CciI	TCATGA	1 cut(s)	174
CfoI	GCGC	5 cut(s)	83, 92, 268, 308, 1690
Cfr10I	RCCGGY	3 cut(s)	83, 1609, 2583
CseI	GACGC	5 cut(s)	119, 949, 2633, 3165, 3522
CspAI	ACCGGT	1 cut(s)	2583
CspCI	CAANNNNNGTGG	2 cut(s)	1212, 1247
DraIII	CACNNNGTG	1 cut(s)	2702
DrdI	GACNNNNNNGTC	2 cut(s)	116, 383
DriI	GACNNNNNGTC	1 cut(s)	3323
DseDI	GACNNNNNNGTC	2 cut(s)	116, 383
EaeI	YGGCCR	2 cut(s)	2041, 2941
Eam1104I	CTCTTC	5 cut(s)	477, 865, 1841, 2488, 3240
Eam1105I	GACNNNNNGTC	1 cut(s)	3323
EarI	CTCTTC	5 cut(s)	477, 865, 1841, 2488, 3240
EciI	GGCGGA	4 cut(s)	12, 78, 222, 429
Ecl136II	GAGCTC	1 cut(s)	172
Eco130I	CCWWGG	4 cut(s)	1344, 2385, 2540, 2632
Eco24I	GRGCYC	4 cut(s)	174, 187, 216, 612
Eco31I	GGTCTC	1 cut(s)	1076
Eco32I	GATATC	1 cut(s)	2263
Eco47I	GGWCC	4 cut(s)	73, 1055, 1745, 2050
Eco53kI	GAGCTC	1 cut(s)	172
Eco57I	CTGAAG	3 cut(s)	1200, 1655, 2475
Eco88I	CYCGRG	4 cut(s)	167, 180, 443, 1749
Eco91I	GGTNACC	2 cut(s)	118, 2702
EcoICRI	GAGCTC	1 cut(s)	172
EcoNI	CCTNNNNNAGG	1 cut(s)	1812
EcoO109I	RGGNCCY	3 cut(s)	1262, 2282, 2764
EcoO65I	GGTNACC	2 cut(s)	118, 2702
EcoRI	GAATTC	1 cut(s)	125
EcoRII	CCWGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
EcoRV	GATATC	1 cut(s)	2263
EcoT14I	CCWWGG	4 cut(s)	1344, 2385, 2540, 2632
EcoT22I	ATGCAT	3 cut(s)	2406, 2967, 3510
EcoT38I	GRGCYC	4 cut(s)	174, 187, 216, 612
ErhI	CCWWGG	4 cut(s)	1344, 2385, 2540, 2632
Esp3I	CGTCTC	2 cut(s)	2503, 3310
FalI	AAGNNNNNCTT	6 cut(s)	1140, 1172, 2868, 2900, 3072, 3104
FaqI	GGGAC	2 cut(s)	14, 3695
FauI	CCCGC	3 cut(s)	106, 233, 727
FauNDI	CATATG	1 cut(s)	1587
FbaI	TGATCA	2 cut(s)	2269, 3160
FriOI	GRGCYC	4 cut(s)	174, 187, 216, 612
FspBI	CTAG	7 cut(s)	1298, 1427, 1536, 1563, 2684, 3231, 3297
GlaI	GCGC	5 cut(s)	82, 91, 267, 307, 1689
GsuI	CTGGAG	3 cut(s)	386, 1701, 3192
HapII	CCGG	7 cut(s)	51, 84, 355, 1610, 1743, 2584, 2843
HgaI	GACGC	5 cut(s)	119, 949, 2633, 3165, 3522
HhaI	GCGC	5 cut(s)	83, 92, 268, 308, 1690
Hin1I	GRCGYC	2 cut(s)	960, 3319
Hin6I	GCGC	5 cut(s)	81, 90, 266, 306, 1688
HinP1I	GCGC	5 cut(s)	81, 90, 266, 306, 1688
HincII	GTYRAC	2 cut(s)	376, 3180
HindII	GTYRAC	2 cut(s)	376, 3180
HindIII	AAGCTT	3 cut(s)	1172, 2190, 3524
HpaII	CCGG	7 cut(s)	51, 84, 355, 1610, 1743, 2584, 2843
HphI	GGTGA	5 cut(s)	112, 737, 1157, 2573, 2714
Hpy166II	GTNNAC	6 cut(s)	376, 668, 1025, 1055, 2398, 3180
Hpy8I	GTNNAC	6 cut(s)	376, 668, 1025, 1055, 2398, 3180
Hpy99I	CGWCG	6 cut(s)	140, 389, 1973, 2649, 3682, 3770
HpyCH4III	ACNGT	6 cut(s)	812, 3151, 3184, 3448, 3501, 3544
HpyCH4IV	ACGT	7 cut(s)	330, 1027, 1138, 2977, 3218, 3319, 3655
HpySE526I	ACGT	7 cut(s)	330, 1027, 1138, 2977, 3218, 3319, 3655
Hsp92I	GRCGYC	2 cut(s)	960, 3319
HspAI	GCGC	5 cut(s)	81, 90, 266, 306, 1688
Kpn2I	TCCGGA	1 cut(s)	1742
KroI	GCCGGC	2 cut(s)	83, 1609
KroNI	GCCGGC	2 cut(s)	85, 1611
Ksp22I	TGATCA	2 cut(s)	2269, 3160
LguI	GCTCTTC	1 cut(s)	2488
LmnI	GCTCC	8 cut(s)	405, 475, 755, 1325, 1834, 2608, 2794, 3173
MaeI	CTAG	7 cut(s)	1298, 1427, 1536, 1563, 2684, 3231, 3297
MaeII	ACGT	7 cut(s)	330, 1027, 1138, 2977, 3218, 3319, 3655
MaeIII	GTNAC	7 cut(s)	118, 1235, 1705, 2077, 2350, 2702, 3214
MbiI	CCGCTC	1 cut(s)	2603
MfeI	CAATTG	2 cut(s)	146, 3517
MflI	RGATCY	7 cut(s)	96, 291, 297, 562, 2236, 2462, 3052
MhlI	GDGCHC	8 cut(s)	174, 187, 216, 362, 612, 1330, 2692, 3605
MlsI	TGGCCA	1 cut(s)	2043
MluNI	TGGCCA	1 cut(s)	2043
MlyI	GAGTC	3 cut(s)	3086, 3233, 3398
MmeI	TCCRAC	3 cut(s)	1303, 2953, 3364
Mox20I	TGGCCA	1 cut(s)	2043
Mph1103I	ATGCAT	3 cut(s)	2406, 2967, 3510
MroI	TCCGGA	1 cut(s)	1742
MroNI	GCCGGC	2 cut(s)	83, 1609
MroXI	GAANNNNTTC	2 cut(s)	1152, 3439
MscI	TGGCCA	1 cut(s)	2043
MseI	TTAA	3 cut(s)	791, 1065, 2207
MslI	CAYNNNNRTG	3 cut(s)	470, 1349, 2390
Msp20I	TGGCCA	1 cut(s)	2043
MspA1I	CMGCKG	2 cut(s)	78, 1843
MspI	CCGG	7 cut(s)	51, 84, 355, 1610, 1743, 2584, 2843
MspR9I	CCNGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
MunI	CAATTG	2 cut(s)	146, 3517
MvaI	CCWGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
MvnI	CGCG	4 cut(s)	113, 347, 1690, 3462
NaeI	GCCGGC	2 cut(s)	85, 1611
NcoI	CCATGG	2 cut(s)	1344, 2385
NdeI	CATATG	1 cut(s)	1587
NgoMIV	GCCGGC	2 cut(s)	83, 1609
NheI	GCTAGC	1 cut(s)	1562
NlaIV	GGNNCC	8 cut(s)	55, 214, 353, 359, 809, 1113, 1263, 1608
NmuCI	GTSAC	4 cut(s)	118, 1705, 2077, 2702
NruI	TCGCGA	1 cut(s)	347
NsiI	ATGCAT	3 cut(s)	2406, 2967, 3510
NspI	RCATGY	6 cut(s)	914, 1450, 1597, 2064, 2404, 3508
NspV	TTCGAA	1 cut(s)	3623
PaeI	GCATGC	1 cut(s)	3508
PaeR7I	CTCGAG	1 cut(s)	1749
PagI	TCATGA	1 cut(s)	174
PaqCI	CACCTGC	1 cut(s)	714
PasI	CCCWGGG	1 cut(s)	209
PciI	ACATGT	1 cut(s)	1446
PciSI	GCTCTTC	1 cut(s)	2488
PcsI	WCGNNNNNNNCGW	2 cut(s)	135, 3661
PdiI	GCCGGC	2 cut(s)	85, 1611
PdmI	GAANNNNTTC	2 cut(s)	1152, 3439
PinAI	ACCGGT	1 cut(s)	2583
PleI	GAGTC	3 cut(s)	3086, 3232, 3397
PpsI	GAGTC	3 cut(s)	3086, 3232, 3397
Ppu21I	YACGTR	4 cut(s)	1028, 1139, 3219, 3656
PscI	ACATGT	1 cut(s)	1446
Psp124BI	GAGCTC	1 cut(s)	174
Psp6I	CCWGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
PspEI	GGTNACC	2 cut(s)	118, 2702
PspGI	CCWGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
PspN4I	GGNNCC	8 cut(s)	55, 214, 353, 359, 809, 1113, 1263, 1608
PspOMI	GGGCCC	1 cut(s)	212
PstI	CTGCAG	2 cut(s)	1842, 3561
PstNI	CAGNNNCTG	3 cut(s)	160, 566, 1208
PsuI	RGATCY	7 cut(s)	96, 291, 297, 562, 2236, 2462, 3052
RruI	TCGCGA	1 cut(s)	347
RseI	CAYNNNNRTG	3 cut(s)	470, 1349, 2390
SacI	GAGCTC	1 cut(s)	174
SapI	GCTCTTC	1 cut(s)	2488
SaqAI	TTAA	3 cut(s)	791, 1065, 2207
ScaI	AGTACT	1 cut(s)	1678
SchI	GAGTC	3 cut(s)	3086, 3233, 3398
ScrFI	CCNGG	8 cut(s)	60, 188, 210, 1059, 1266, 1866, 2823, 3203
SduI	GDGCHC	8 cut(s)	174, 187, 216, 362, 612, 1330, 2692, 3605
SfcI	CTRYAG	2 cut(s)	1838, 3557
Sfr274I	CTCGAG	1 cut(s)	1749
SfuI	TTCGAA	1 cut(s)	3623
SinI	GGWCC	4 cut(s)	73, 1055, 1745, 2050
SlaI	CTCGAG	1 cut(s)	1749
SmiMI	CAYNNNNRTG	3 cut(s)	470, 1349, 2390
SmlI	CTYRAG	1 cut(s)	1749
SmoI	CTYRAG	1 cut(s)	1749
SphI	GCATGC	1 cut(s)	3508
SspMI	CTAG	7 cut(s)	1298, 1427, 1536, 1563, 2684, 3231, 3297
SstI	GAGCTC	1 cut(s)	174
StyD4I	CCNGG	8 cut(s)	58, 186, 208, 1057, 1264, 1864, 2821, 3201
StyI	CCWWGG	4 cut(s)	1344, 2385, 2540, 2632
TaaI	ACNGT	6 cut(s)	812, 3151, 3184, 3448, 3501, 3544
TaiI	ACGT	7 cut(s)	333, 1030, 1141, 2980, 3221, 3322, 3658
TatI	WGTACW	5 cut(s)	907, 1449, 1676, 2167, 3539
TauI	GCSGC	5 cut(s)	166, 247, 436, 3652, 3722
Tru1I	TTAA	3 cut(s)	791, 1065, 2207
Tru9I	TTAA	3 cut(s)	791, 1065, 2207
TseFI	GTSAC	4 cut(s)	118, 1705, 2077, 2702
Tsp45I	GTSAC	4 cut(s)	118, 1705, 2077, 2702
TspGWI	ACGGA	6 cut(s)	533, 1277, 2380, 3362, 3695, 3721
VpaK11BI	GGWCC	4 cut(s)	73, 1055, 1745, 2050
XagI	CCTNNNNNAGG	1 cut(s)	1812
XapI	RAATTY	9 cut(s)	125, 1161, 1228, 1292, 1466, 1766, 1989, 2135, 2379
XceI	RCATGY	6 cut(s)	914, 1450, 1597, 2064, 2404, 3508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	206
XhoI	CTCGAG	1 cut(s)	1749
XmnI	GAANNNNTTC	2 cut(s)	1152, 3439
XspI	CTAG	7 cut(s)	1298, 1427, 1536, 1563, 2684, 3231, 3297
ZraI	GACGTC	1 cut(s)	3320
ZrmI	AGTACT	1 cut(s)	1678
Zsp2I	ATGCAT	3 cut(s)	2406, 2967, 3510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.