Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006161

Physical Location & Seq

Genomic Coordinates Reverse (-)

17994342 .. 18008972

Gene Structure 14631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032703

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3780 bp

ATGGCGCAGCAGCAGCAGCAGCAGCCTCCGTCGGTCCCGCCGCAATTGCCGACCGCCGGCGCCAGCGGGCCCGTCGGAGGCGTCCAGGTGCGGTGCGCCGGCTGCCACAAGATCCTCACCATCGCGGCCGGGGTCACCGAATTCGTCTGCGGCACGTGTCAATTGCCGCAGATGCTTCCGCCGGAGCTGATGAGCCGGGCCCAGGCCCATAACCAGCCCAACAAGGGCCCGGTTCCCCCGCCCCCGCCGCGGCCGCAGCCGCAGCACGTGGCCGCCCACGGCGTCGATCCTACGAAGATCCAGCTCCCCTGCGCCAACTGCAAGGCGATTCTCAATGTGCCTCATGGACTCTCTCGGTTTCAGTGCCCTCAGTGCCATGTTGACCTCGCCGTCGATGTGTCCAAGCTCAAAGAGTTCTTGCTGCCACCACCGCCTTCCGAGGAGGTCAACGAGGTTGCCATTGAAGTGGAGCGAGAAGAAGATGAAGGTGGCCTGGCCGGGGAAACATTTACAGATTATCGCCCTCCAAAGCTGTCAATTGGACCTCCTCATCCAGATCCCGTTGTGGAAACATCATCCCTGTCGGCTGTGCAGCCACCTGAGCCCACTTACGATCTAACAATCAAGGATGATTTGGAAAATTCAAAGGCTTTATCATGTTTGCAGATTGAGACATTGGTTTATGCCTGTCAGAGACACCTCCAGCACCTTCCAAGTGGTGTTAGGGCTGGATTCTTTGTTGGAGATGGAGCTGGTGTGGGTAAAGGTCGAACAATTGCAGGGTTAATTTGGGAAAATTGGCACCATGGGAGGAGAAAGGCAGTGTGGATTTCTGTTGGTTCAGACTTGAAGTTTGATGCAAGAAGAGACTTAGATGATGTAGGCGCAGCTTGTATAGAAGTTCATGCTTTGAACAAGCTGCCATATTCTAAGCTTGATTCCAAGTCTGTTGGCATCAAGGAGGGAGTTATATTCCTCACATACAGCAGCCTTATAGCATCTTCTGAGAAAGGTCGTTCACGCATGCAGCAGCTTGTGCAGTGGTGTGGATCAGGATATGATGGTCTTATCGTCTTTGACGAGTGCCACAAAGCCAAAAATTTGATACCTGAAGCTGGAAGTCAGCCAACACGAACCGGTGAAGCAGTTCTTGACATTCAGGCTCGACTGCCTGAAGCTCGGGTTATTTATTGCTCGGCAACTGGTGCATCAGAACCACGCAACTTGGGTTACATGGTTCGGCTTGGTCTTTGGGGCCCTGGAACATCTTTTTCTGAGTTCCGTGAATTTCTAGGTGCTCTTGAGAAAGGAGGTGTTGGTGCACTTGAACTTGTTGCCATGGACATGAAAGCAAGGGGGATGTATGTCTGTCGTACTCTTAGCTATAAAGGAGCAGAGTTTGAAGTTGTTGAGGCACCACTAGAAGATGAAATGATGGAAATGTACAAAAAGGCAGCAGAATTTTGGACAGAGCTGCGAGTTGAAATACTAACAGCTACTGCCTATCTCACTAATGAAAAGCCCATTTCTAGTCAAGTCTGGCGATTGTATTGGGCTAGTCATCAGCGTTTCTTTAGACATATGTGTATGTCGGCTAAGGTTCCTGCTGCTGTAAGACTAGCGAAGAAAGCATTGATGGATGACAAGTGTGTCGTAATTGGCCTTCAGAGTACTGGAGAGGCACGAACTGAAGAAGCTGTGACTAAATATGGTCTTGAGCTTGATGATTTTATTTCGGGACCTCGAGAACTGTTGCTGAAATTTGTTGAAGAAAACTATCCTTTGCCTGAAAAGCCTGATCCCGTCACAGGAGAAGAAAGTGTAAAGGAGCTTCAACGGAAGAGGCACTCAGCAAGTCCTGGTGTTTCGATGAAAGGGAGAGTGAGGAAAGTTGCAAAAGTGCAACCGCCAATTGATAACGAAAGTGACGAAGAATCTGAAACTGATTCTGCTGTTGAATCTACTGAATCAGATGACGAGTTTCAAATCTGTGAAATCTGCAATGCTGAAACGGAAAGAAAGAAATTGCTCCACTGTTCCTGTTGTGGCCAACTGGTCCATGCTGAATGCCTTATCCCACCTGTGATAGATGAGGTATCAGAAGATTGGTCATGTCATTCGTGCAAGGAAAAAACAGATGAATATCTTCAACGGAGAGAGCAGTATATAGCTGAATTGAAGAAGAGGTATGAAGCAGCTTTGGAGCGTAAGTCAAAAATTCTGGGGATAATTCGTTCCTTGGATCTTCCAAACAATCCTTTGGACGATATCATTGATCAGCTTGGAGGCCCTGATAAGGTAGCTGAAATGACAGGAAGGCGAGGTATGCTTGTGAGGGCATCTAATGGTAAAGGTGTTACCTATCAGGCTCGAAACACGAAGGAGGTTTCCATGGAGATGGTCAACATGCATGAGAAACAGCTTTTCATGGATGGCAAAAAGTTTGTTGCTATAATTTCTGAGGCTGGGTCAGCTGGTGTTTCTTTGCAGGCAGATAGAAGAGCTGTAAATCAGAAAAGAAGGGTACATCTAACCCTGGAACTTCCTTGGAGTGCCGATCGAGCGATTCAACAGTTCGGAAGAACTCACCGGTCAAATCAAGCTTCTGCTCCTGAATACAGGCTACTCTTTACTAACCTCGGTGGAGAACGTCGGTTTGCATCCATTGTTGCAAAGAGATTACAATCTCTTGGAGCCCTAACTCAGGGTGATCGCAGGGCAGGCCCATCATTGAGTGCCTATAATTATGATAGTGCTTATGGAAAGAAGGCATTAATATTGATGTATAGAGGAATATTGGAGCAGGAAGCTCTGCCTGTTGTACCACCAGGATGTTCATCTGAAGACCCGGAGACAATCCAAGAATTTATTGTGGAGGCAAGAGCTGCTCTTGTGTTTGTAGGAATAATTAGGGATGCTAAAGATTCTGGCAAGCTCTCTGGTCGTGTAGCTGATTCAGACATGCATGATGTAGGACGTTTCCTCAATCGGATTTTAGGATTGCCACCTAAAGTACAAAATAGGCTGTTCGAGTTATTTGTTAGTATTTTGGATCTTCTTGTCCATAATGCACGCATTGAAGGAAATCTTGACTCTGGGATTGTTGATATGAAAGCTAATGTTATAGAGTTACAAGGAACTCCAAAGACTGTCCATGTTGACGAAATGTCTGGAGCTTCAACTGTGCTCTTCACTTTTACCCTGGATCGTGGCATCACATGGGAGTCTGCAAGTGCCATGCTTGAAGGTAAACAAGAGGAGGGGCTTAGTTGTGCCCATGATGGCTTCTATGAATCTAGAAGGGAGTGGATGGGGAGGCGCCATACCATATTAGCCTTTGAAAGTTCTACTTCTGGGTCCTTTAAGATAGTCCGTCCGGCAGTTGGAGAGTCAGTGAGAGAAATGTCTCTATCTGAGCTAAAAAGCAAATACAGAAGAACATCATCGTTGGAGAAGGCTCGCAGCGGTTGGAAAGATGAATATGAAGTTTCATCCAAACAGTGCATGCATGGTCCCAAGTGTAAGGTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGTCTCATTCTTCCTGTATGGGGCACTATTGAGAAAGCTCTTTCTAAGCAGTCCCGGCAAAGCCACAGGCGGCTACGTGTTGTGCGTATAGAAACCACAACAGATAACCAGAGAATTGTTGGTCTCTTTGTCCCCAATGCAGCTGTTGAAGCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATTGAAGATTCACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1260

Amino Acids

139.35

Weight (kDa)

6.25

Isoelectric Point (pI)

52.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_FGT1_1	PF23547	28 - 62	3.5e-18	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1	PF23547	100 - 129	6.4e-10	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2	PF23548	100 - 133	3.8e-17	FORGETTER1 second zinc ribbon domain
AAA_34	PF13872	183 - 483	1.2e-134	P-loop containing NTP hydrolase pore-1
Helicase_C_4	PF13871	752 - 1036	2.4e-104	C-terminal domain on Strawberry notch homologue
SBNO	PF25373	1073 - 1185	1.7e-18	SBNO alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006161)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79350
fragaria_vesca	FvH4_3g13920 FvH4_3g13920 FvH4_3g13920 FvH4_3g13920
malus_domestica	MD05G1239900.v1.1 MD10G1218000.v1.1
prunus_persica	Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1
pyrus_communis	pycom05g21600 pycom10g18380
rosa_chinensis	RchiOBHm_Chr5g0023151
rosa_laevigata	RLG00000032703
rosa_multiflora	Rmu_co8366291.1_g000001 Rmu_sc0007300.1_g000019 Rmu_sc0015109.1_g000002 Rmu_sc0033377.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056190
rosa_rugosa	Rorug05G0077600
rosa_samantha	Rh5AG163600 Rh5BG163000 Rh5CG177800 Rh5DG164200
rosa_wichuraiana	Rw5G014760 Rw5G015070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	389, 1649
AccB1I	GGYRCC	4 cut(s)	59, 801, 1414, 3318
AccII	CGCG	2 cut(s)	125, 250
AclWI	GGATC	9 cut(s)	106, 281, 292, 551, 1057, 1793, 2250, 3060, 3213
AcoI	YGGCCR	5 cut(s)	126, 251, 270, 495, 2047
AcsI	RAATTY	8 cut(s)	140, 640, 1099, 1286, 1460, 1760, 2217, 2864
AcuI	CTGAAG	5 cut(s)	1131, 1194, 1649, 1710, 2862
AcvI	CACGTG	2 cut(s)	156, 268
AcyI	GRCGYC	4 cut(s)	60, 81, 282, 3319
AfaI	GTAC	8 cut(s)	1375, 1445, 1672, 2526, 2823, 3015, 3541, 3735
AflIII	ACRYGT	2 cut(s)	155, 3655
AgeI	ACCGGT	2 cut(s)	1136, 2589
AhdI	GACNNNNNGTC	1 cut(s)	3166
AjnI	CCWGG	8 cut(s)	84, 201, 492, 1258, 1858, 2535, 2827, 3201
AloI	GAACNNNNNNTCC	2 cut(s)	2218, 2250
Alw21I	GWGCWC	3 cut(s)	1300, 1324, 3189
Alw26I	GTCTC	7 cut(s)	665, 688, 861, 2846, 3411, 3587, 3707
Alw44I	GTGCAC	1 cut(s)	1320
AlwI	GGATC	9 cut(s)	106, 281, 292, 551, 1057, 1793, 2250, 3060, 3213
AlwNI	CAGNNNCTG	1 cut(s)	1499
Ama87I	CYCGRG	2 cut(s)	1179, 1743
ApaI	GGGCCC	4 cut(s)	72, 202, 230, 1259
ApaLI	GTGCAC	1 cut(s)	1320
ApoI	RAATTY	8 cut(s)	140, 640, 1099, 1286, 1460, 1760, 2217, 2864
ArsI	GACNNNNNNTTYG	2 cut(s)	836, 868
AseI	ATTAAT	1 cut(s)	2774
AsiGI	ACCGGT	2 cut(s)	1136, 2589
Asp700I	GAANNNNTTC	2 cut(s)	1146, 2145
AspLEI	GCGC	6 cut(s)	7, 62, 98, 314, 887, 3321
AsuC2I	CCSGG	6 cut(s)	130, 197, 230, 499, 2849, 3634
AsuHPI	GGTGA	5 cut(s)	109, 127, 1151, 2579, 2720
AvaI	CYCGRG	2 cut(s)	1179, 1743
AvaII	GGWCC	6 cut(s)	34, 542, 1739, 2056, 3357, 3512
BaeGI	GKGCMC	8 cut(s)	72, 202, 230, 368, 1259, 1324, 3277, 3605
BalI	TGGCCA	1 cut(s)	2049
BanI	GGYRCC	4 cut(s)	59, 801, 1414, 3318
BanII	GRGCYC	6 cut(s)	72, 202, 230, 606, 1259, 2698
BarI	GAAGNNNNNNTAC	4 cut(s)	1647, 1679, 1815, 1847
BbrPI	CACGTG	2 cut(s)	156, 268
BbsI	GAAGAC	2 cut(s)	2850, 3559
Bbv12I	GWGCWC	3 cut(s)	1300, 1324, 3189
BceAI	ACGGC	3 cut(s)	295, 374, 3716
BciT130I	CCWGG	8 cut(s)	86, 203, 494, 1260, 1860, 2537, 2829, 3203
BclI	TGATCA	1 cut(s)	2275
BcnI	CCSGG	6 cut(s)	130, 197, 230, 499, 2849, 3634
BcoDI	GTCTC	7 cut(s)	665, 688, 861, 2846, 3411, 3587, 3707
BfaI	CTAG	6 cut(s)	1292, 1421, 1530, 1557, 1619, 3297
BfmI	CTRYAG	1 cut(s)	3557
BfoI	RGCGCY	2 cut(s)	63, 3322
BmcAI	AGTACT	1 cut(s)	1672
Bme18I	GGWCC	6 cut(s)	34, 542, 1739, 2056, 3357, 3512
BmeRI	GACNNNNNGTC	1 cut(s)	3166
BmeT110I	CYCGRG	2 cut(s)	1179, 1743
BmsI	GCATC	9 cut(s)	162, 847, 963, 1007, 1217, 2348, 2669, 2905, 3222
BpiI	GAAGAC	2 cut(s)	2850, 3559
BpmI	CTGGAG	3 cut(s)	686, 1695, 3192
Bpu10I	CCTNAGC	2 cut(s)	600, 1596
BpuEI	CTTGAG	2 cut(s)	1322, 1736
BpuMI	CCSGG	6 cut(s)	130, 197, 230, 499, 2849, 3634
BsaAI	YACGTR	3 cut(s)	156, 268, 3656
BsaBI	GATNNNNATC	3 cut(s)	2142, 2683, 2836
BsaHI	GRCGYC	4 cut(s)	60, 81, 282, 3319
BsaI	GGTCTC	2 cut(s)	3587, 3707
BsaWI	WCCGGW	2 cut(s)	1136, 2589
BsaXI	ACNNNNNCTCC	6 cut(s)	741, 771, 2195, 2225, 3378, 3408
Bse118I	RCCGGY	4 cut(s)	56, 98, 1136, 2589
Bse1I	ACTGG	3 cut(s)	1207, 1678, 2058
Bse3DI	GCAATG	1 cut(s)	2008
Bse8I	GATNNNNATC	3 cut(s)	2142, 2683, 2836
BseBI	CCWGG	8 cut(s)	86, 203, 494, 1260, 1860, 2537, 2829, 3203
BseJI	GATNNNNATC	3 cut(s)	2142, 2683, 2836
BseMI	GCAATG	1 cut(s)	2008
BseMII	CTCAG	8 cut(s)	383, 591, 996, 1266, 1863, 2451, 2717, 3405
BseNI	ACTGG	3 cut(s)	1207, 1678, 2058
BseRI	GAGGAG	4 cut(s)	455, 537, 826, 3272
BseSI	GKGCMC	8 cut(s)	72, 202, 230, 368, 1259, 1324, 3277, 3605
BseX3I	CGGCCG	2 cut(s)	126, 251
BseYI	CCCAGC	1 cut(s)	2465
BsgI	GTGCAG	2 cut(s)	611, 1058
Bsh1236I	CGCG	2 cut(s)	125, 250
Bsh1285I	CGRYCG	4 cut(s)	54, 129, 254, 2560
BshNI	GGYRCC	4 cut(s)	59, 801, 1414, 3318
BshTI	ACCGGT	2 cut(s)	1136, 2589
BsiEI	CGRYCG	4 cut(s)	54, 129, 254, 2560
BsiHKAI	GWGCWC	3 cut(s)	1300, 1324, 3189
BsiHKCI	CYCGRG	2 cut(s)	1179, 1743
BslFI	GGGAC	5 cut(s)	20, 1752, 3498, 3616, 3695
BsmAI	GTCTC	7 cut(s)	665, 688, 861, 2846, 3411, 3587, 3707
BsmFI	GGGAC	5 cut(s)	20, 1752, 3498, 3616, 3695
BsmI	GAATGC	1 cut(s)	2072
Bso31I	GGTCTC	2 cut(s)	3587, 3707
BsoBI	CYCGRG	2 cut(s)	1179, 1743
Bsp120I	GGGCCC	4 cut(s)	68, 198, 226, 1255
Bsp1407I	TGTACA	1 cut(s)	1443
Bsp19I	CCATGG	3 cut(s)	805, 1338, 2391
BspCNI	CTCAG	8 cut(s)	382, 592, 997, 1267, 1862, 2452, 2716, 3406
BspFNI	CGCG	2 cut(s)	125, 250
BspMAI	CTGCAG	1 cut(s)	3561
BspPI	GGATC	9 cut(s)	106, 281, 292, 551, 1057, 1793, 2250, 3060, 3213
BspQI	GCTCTTC	2 cut(s)	2494, 3194
BspT107I	GGYRCC	4 cut(s)	59, 801, 1414, 3318
BspTNI	GGTCTC	2 cut(s)	3587, 3707
BsrDI	GCAATG	1 cut(s)	2008
BsrFI	RCCGGY	4 cut(s)	56, 98, 1136, 2589
BsrGI	TGTACA	1 cut(s)	1443
BsrI	ACTGG	3 cut(s)	1207, 1678, 2058
BssAI	RCCGGY	4 cut(s)	56, 98, 1136, 2589
BssNI	GRCGYC	4 cut(s)	60, 81, 282, 3319
BssT1I	CCWWGG	5 cut(s)	805, 1338, 2238, 2391, 2546
Bst2UI	CCWGG	8 cut(s)	86, 203, 494, 1260, 1860, 2537, 2829, 3203
Bst4CI	ACNGT	8 cut(s)	1752, 2036, 2574, 3151, 3184, 3501, 3544, 3777
Bst6I	CTCTTC	5 cut(s)	859, 1835, 2177, 2494, 3194
BstACI	GRCGYC	4 cut(s)	60, 81, 282, 3319
BstAPI	GCANNNNNTGC	3 cut(s)	1036, 1205, 2885
BstAUI	TGTACA	1 cut(s)	1443
BstBAI	YACGTR	3 cut(s)	156, 268, 3656
BstDSI	CCRYGG	5 cut(s)	248, 277, 805, 1338, 2391
BstEII	GGTNACC	1 cut(s)	133
BstENI	CCTNNNNNAGG	1 cut(s)	2702
BstFNI	CGCG	2 cut(s)	125, 250
BstH2I	RGCGCY	2 cut(s)	63, 3322
BstHHI	GCGC	6 cut(s)	7, 62, 98, 314, 887, 3321
BstMAI	GTCTC	7 cut(s)	665, 688, 861, 2846, 3411, 3587, 3707
BstMCI	CGRYCG	4 cut(s)	54, 129, 254, 2560
BstNI	CCWGG	8 cut(s)	86, 203, 494, 1260, 1860, 2537, 2829, 3203
BstNSI	RCATGY	4 cut(s)	1027, 2410, 2965, 3508
BstPI	GGTNACC	1 cut(s)	133
BstSFI	CTRYAG	1 cut(s)	3557
BstSLI	GKGCMC	8 cut(s)	72, 202, 230, 368, 1259, 1324, 3277, 3605
BstUI	CGCG	2 cut(s)	125, 250
BstV2I	GAAGAC	2 cut(s)	2850, 3559
BstX2I	RGATCY	5 cut(s)	111, 297, 556, 2242, 3052
BstXI	CCANNNNNNTGG	2 cut(s)	466, 2398
BstYI	RGATCY	5 cut(s)	111, 297, 556, 2242, 3052
BstZI	CGGCCG	2 cut(s)	126, 251
BtgI	CCRYGG	5 cut(s)	248, 277, 805, 1338, 2391
BtgZI	GCGATG	1 cut(s)	106
BtsI	GCAGTG	2 cut(s)	828, 1046
BtsIMutI	CAGTG	7 cut(s)	368, 377, 828, 1046, 2032, 3399, 3506
CaiI	CAGNNNCTG	1 cut(s)	1499
CciNI	GCGGCCGC	1 cut(s)	251
CfoI	GCGC	6 cut(s)	7, 62, 98, 314, 887, 3321
Cfr10I	RCCGGY	4 cut(s)	56, 98, 1136, 2589
Cfr42I	CCGCGG	1 cut(s)	251
CseI	GACGC	2 cut(s)	70, 271
Csp6I	GTAC	8 cut(s)	1374, 1444, 1671, 2525, 2822, 3014, 3540, 3734
CspAI	ACCGGT	2 cut(s)	1136, 2589
CspCI	CAANNNNNGTGG	2 cut(s)	1206, 1241
CviQI	GTAC	8 cut(s)	1374, 1444, 1671, 2525, 2822, 3014, 3540, 3734
DinI	GGCGCC	2 cut(s)	61, 3320
DrdI	GACNNNNNNGTC	2 cut(s)	389, 1649
DriI	GACNNNNNGTC	1 cut(s)	3166
DseDI	GACNNNNNNGTC	2 cut(s)	389, 1649
EaeI	YGGCCR	5 cut(s)	126, 251, 270, 495, 2047
EagI	CGGCCG	2 cut(s)	126, 251
Eam1104I	CTCTTC	5 cut(s)	859, 1835, 2177, 2494, 3194
Eam1105I	GACNNNNNGTC	1 cut(s)	3166
EarI	CTCTTC	5 cut(s)	859, 1835, 2177, 2494, 3194
EciI	GGCGGA	1 cut(s)	168
EclXI	CGGCCG	2 cut(s)	126, 251
Eco130I	CCWWGG	5 cut(s)	805, 1338, 2238, 2391, 2546
Eco24I	GRGCYC	6 cut(s)	72, 202, 230, 606, 1259, 2698
Eco31I	GGTCTC	2 cut(s)	3587, 3707
Eco32I	GATATC	1 cut(s)	2269
Eco47I	GGWCC	6 cut(s)	34, 542, 1739, 2056, 3357, 3512
Eco52I	CGGCCG	2 cut(s)	126, 251
Eco57I	CTGAAG	5 cut(s)	1131, 1194, 1649, 1710, 2862
Eco72I	CACGTG	2 cut(s)	156, 268
Eco88I	CYCGRG	2 cut(s)	1179, 1743
Eco91I	GGTNACC	1 cut(s)	133
EcoNI	CCTNNNNNAGG	1 cut(s)	2702
EcoO109I	RGGNCCY	6 cut(s)	226, 1255, 1256, 1739, 2288, 3357
EcoO65I	GGTNACC	1 cut(s)	133
EcoRI	GAATTC	1 cut(s)	140
EcoRII	CCWGG	8 cut(s)	84, 201, 492, 1258, 1858, 2535, 2827, 3201
EcoRV	GATATC	1 cut(s)	2269
EcoT14I	CCWWGG	5 cut(s)	805, 1338, 2238, 2391, 2546
EcoT22I	ATGCAT	3 cut(s)	2412, 2967, 3510
EcoT38I	GRGCYC	6 cut(s)	72, 202, 230, 606, 1259, 2698
EgeI	GGCGCC	2 cut(s)	61, 3320
EheI	GGCGCC	2 cut(s)	61, 3320
ErhI	CCWWGG	5 cut(s)	805, 1338, 2238, 2391, 2546
FalI	AAGNNNNNCTT	8 cut(s)	1134, 1166, 3072, 3104, 3334, 3366, 3720, 3752
FaqI	GGGAC	5 cut(s)	20, 1752, 3498, 3616, 3695
FauI	CCCGC	4 cut(s)	45, 59, 246, 252
FauNDI	CATATG	1 cut(s)	1581
FbaI	TGATCA	1 cut(s)	2275
FriOI	GRGCYC	6 cut(s)	72, 202, 230, 606, 1259, 2698
FspBI	CTAG	6 cut(s)	1292, 1421, 1530, 1557, 1619, 3297
GlaI	GCGC	6 cut(s)	6, 61, 97, 313, 886, 3320
GsaI	CCCAGC	1 cut(s)	2469
GsuI	CTGGAG	3 cut(s)	686, 1695, 3192
HaeII	RGCGCY	2 cut(s)	63, 3322
HgaI	GACGC	2 cut(s)	70, 271
HhaI	GCGC	6 cut(s)	7, 62, 98, 314, 887, 3321
Hin1I	GRCGYC	4 cut(s)	60, 81, 282, 3319
Hin6I	GCGC	6 cut(s)	5, 60, 96, 312, 885, 3319
HinP1I	GCGC	6 cut(s)	5, 60, 96, 312, 885, 3319
HincII	GTYRAC	4 cut(s)	382, 448, 2404, 3160
HindII	GTYRAC	4 cut(s)	382, 448, 2404, 3160
HindIII	AAGCTT	2 cut(s)	932, 2601
HphI	GGTGA	5 cut(s)	109, 127, 1151, 2579, 2720
Hpy166II	GTNNAC	7 cut(s)	382, 448, 1019, 1322, 2404, 3160, 3251
Hpy8I	GTNNAC	7 cut(s)	382, 448, 1019, 1322, 2404, 3160, 3251
Hpy99I	CGWCG	5 cut(s)	34, 77, 287, 395, 2655
HpyCH4III	ACNGT	8 cut(s)	1752, 2036, 2574, 3151, 3184, 3501, 3544, 3777
HpyCH4IV	ACGT	5 cut(s)	155, 267, 2650, 2977, 3655
HpySE526I	ACGT	5 cut(s)	155, 267, 2650, 2977, 3655
Hsp92I	GRCGYC	4 cut(s)	60, 81, 282, 3319
HspAI	GCGC	6 cut(s)	5, 60, 96, 312, 885, 3319
KasI	GGCGCC	2 cut(s)	59, 3318
KroI	GCCGGC	2 cut(s)	56, 98
KroNI	GCCGGC	2 cut(s)	58, 100
Ksp22I	TGATCA	1 cut(s)	2275
KspI	CCGCGG	1 cut(s)	251
LguI	GCTCTTC	2 cut(s)	2494, 3194
LweI	GCATC	9 cut(s)	162, 847, 963, 1007, 1217, 2348, 2669, 2905, 3222
MaeI	CTAG	6 cut(s)	1292, 1421, 1530, 1557, 1619, 3297
MaeII	ACGT	5 cut(s)	155, 267, 2650, 2977, 3655
MaeIII	GTNAC	7 cut(s)	133, 1229, 1699, 1804, 1925, 2356, 3129
MfeI	CAATTG	5 cut(s)	44, 161, 537, 774, 1911
MflI	RGATCY	5 cut(s)	111, 297, 556, 2242, 3052
MlsI	TGGCCA	1 cut(s)	2049
MluNI	TGGCCA	1 cut(s)	2049
Mly113I	GGCGCC	2 cut(s)	60, 3319
MlyI	GAGTC	4 cut(s)	342, 3086, 3233, 3398
MmeI	TCCRAC	5 cut(s)	55, 721, 3364, 3429, 3449
Mox20I	TGGCCA	1 cut(s)	2049
Mph1103I	ATGCAT	3 cut(s)	2412, 2967, 3510
MreI	CGCCGGCG	1 cut(s)	56
MroNI	GCCGGC	2 cut(s)	56, 98
MroXI	GAANNNNTTC	2 cut(s)	1146, 2145
MscI	TGGCCA	1 cut(s)	2049
MseI	TTAA	3 cut(s)	785, 2774, 3363
MslI	CAYNNNNRTG	4 cut(s)	464, 1343, 2396, 2830
Msp20I	TGGCCA	1 cut(s)	2049
MspA1I	CMGCKG	5 cut(s)	66, 250, 2474, 3465, 3722
MunI	CAATTG	5 cut(s)	44, 161, 537, 774, 1911
Mva1269I	GAATGC	1 cut(s)	2072
MvaI	CCWGG	8 cut(s)	86, 203, 494, 1260, 1860, 2537, 2829, 3203
MvnI	CGCG	2 cut(s)	125, 250
NaeI	GCCGGC	2 cut(s)	58, 100
NarI	GGCGCC	2 cut(s)	60, 3319
NciI	CCSGG	6 cut(s)	130, 197, 230, 499, 2849, 3634
NcoI	CCATGG	3 cut(s)	805, 1338, 2391
NdeI	CATATG	1 cut(s)	1581
NgoMIV	GCCGGC	2 cut(s)	56, 98
NmeAIII	GCCGAG	1 cut(s)	1175
NmuCI	GTSAC	4 cut(s)	133, 1699, 1804, 1925
NotI	GCGGCCGC	1 cut(s)	251
NsiI	ATGCAT	3 cut(s)	2412, 2967, 3510
NspI	RCATGY	4 cut(s)	1027, 2410, 2965, 3508
PaeI	GCATGC	2 cut(s)	1027, 3508
PaeR7I	CTCGAG	1 cut(s)	1743
PciSI	GCTCTTC	2 cut(s)	2494, 3194
PcsI	WCGNNNNNNNCGW	3 cut(s)	1077, 1926, 3661
PctI	GAATGC	1 cut(s)	2072
PdiI	GCCGGC	2 cut(s)	58, 100
PdmI	GAANNNNTTC	2 cut(s)	1146, 2145
PinAI	ACCGGT	2 cut(s)	1136, 2589
Ple19I	CGATCG	1 cut(s)	2560
PleI	GAGTC	4 cut(s)	342, 3086, 3232, 3397
PluTI	GGCGCC	2 cut(s)	63, 3322
PmaCI	CACGTG	2 cut(s)	156, 268
PmlI	CACGTG	2 cut(s)	156, 268
PpsI	GAGTC	4 cut(s)	342, 3086, 3232, 3397
Ppu21I	YACGTR	3 cut(s)	156, 268, 3656
PpuMI	RGGWCCY	2 cut(s)	1739, 3357
PshBI	ATTAAT	1 cut(s)	2774
Psp5II	RGGWCCY	2 cut(s)	1739, 3357
Psp6I	CCWGG	8 cut(s)	84, 201, 492, 1258, 1858, 2535, 2827, 3201
PspCI	CACGTG	2 cut(s)	156, 268
PspEI	GGTNACC	1 cut(s)	133
PspFI	CCCAGC	1 cut(s)	2465
PspGI	CCWGG	8 cut(s)	84, 201, 492, 1258, 1858, 2535, 2827, 3201
PspOMI	GGGCCC	4 cut(s)	68, 198, 226, 1255
PspPPI	RGGWCCY	2 cut(s)	1739, 3357
PstI	CTGCAG	1 cut(s)	3561
PstNI	CAGNNNCTG	1 cut(s)	1499
PsuI	RGATCY	5 cut(s)	111, 297, 556, 2242, 3052
PvuI	CGATCG	1 cut(s)	2560
PvuII	CAGCTG	2 cut(s)	2474, 3722
RsaI	GTAC	8 cut(s)	1375, 1445, 1672, 2526, 2823, 3015, 3541, 3735
RsaNI	GTAC	8 cut(s)	1374, 1444, 1671, 2525, 2822, 3014, 3540, 3734
RseI	CAYNNNNRTG	4 cut(s)	464, 1343, 2396, 2830
SacII	CCGCGG	1 cut(s)	251
SapI	GCTCTTC	2 cut(s)	2494, 3194
SaqAI	TTAA	3 cut(s)	785, 2774, 3363
ScaI	AGTACT	1 cut(s)	1672
SchI	GAGTC	4 cut(s)	342, 3086, 3233, 3398
SfaNI	GCATC	9 cut(s)	162, 847, 963, 1007, 1217, 2348, 2669, 2905, 3222
SfcI	CTRYAG	1 cut(s)	3557
SfoI	GGCGCC	2 cut(s)	61, 3320
Sfr274I	CTCGAG	1 cut(s)	1743
Sfr303I	CCGCGG	1 cut(s)	251
SgrAI	CRCCGGYG	1 cut(s)	56
SgrBI	CCGCGG	1 cut(s)	251
SinI	GGWCC	6 cut(s)	34, 542, 1739, 2056, 3357, 3512
SlaI	CTCGAG	1 cut(s)	1743
SmiMI	CAYNNNNRTG	4 cut(s)	464, 1343, 2396, 2830
SmlI	CTYRAG	3 cut(s)	1301, 1715, 1743
SmoI	CTYRAG	3 cut(s)	1301, 1715, 1743
SphI	GCATGC	2 cut(s)	1027, 3508
SspDI	GGCGCC	2 cut(s)	59, 3318
SspI	AATATT	2 cut(s)	2778, 2796
SspMI	CTAG	6 cut(s)	1292, 1421, 1530, 1557, 1619, 3297
StyI	CCWWGG	5 cut(s)	805, 1338, 2238, 2391, 2546
TaaI	ACNGT	8 cut(s)	1752, 2036, 2574, 3151, 3184, 3501, 3544, 3777
TaiI	ACGT	5 cut(s)	158, 270, 2653, 2980, 3658
TaqI	TCGA	9 cut(s)	285, 393, 769, 1165, 1744, 1868, 2371, 2560, 3030
TaqII	GACCGA	1 cut(s)	22
TatI	WGTACW	4 cut(s)	1443, 1670, 3013, 3539
Tru1I	TTAA	3 cut(s)	785, 2774, 3363
Tru9I	TTAA	3 cut(s)	785, 2774, 3363
TscAI	CASTG	7 cut(s)	368, 377, 828, 1046, 2039, 3399, 3506
TseFI	GTSAC	4 cut(s)	133, 1699, 1804, 1925
Tsp45I	GTSAC	4 cut(s)	133, 1699, 1804, 1925
TspGWI	ACGGA	6 cut(s)	18, 1271, 1852, 2027, 2167, 3362
TspRI	CASTG	7 cut(s)	368, 377, 828, 1046, 2039, 3399, 3506
VneI	GTGCAC	1 cut(s)	1320
VpaK11BI	GGWCC	6 cut(s)	34, 542, 1739, 2056, 3357, 3512
VspI	ATTAAT	1 cut(s)	2774
XagI	CCTNNNNNAGG	1 cut(s)	2702
XapI	RAATTY	8 cut(s)	140, 640, 1099, 1286, 1460, 1760, 2217, 2864
XbaI	TCTAGA	1 cut(s)	3296
XceI	RCATGY	4 cut(s)	1027, 2410, 2965, 3508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3695
XhoI	CTCGAG	1 cut(s)	1743
XmnI	GAANNNNTTC	2 cut(s)	1146, 2145
XspI	CTAG	6 cut(s)	1292, 1421, 1530, 1557, 1619, 3297
ZrmI	AGTACT	1 cut(s)	1672
Zsp2I	ATGCAT	3 cut(s)	2412, 2967, 3510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.