Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006161

Physical Location & Seq

Genomic Coordinates Forward (+)

31618171 .. 31631511

Gene Structure 13341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1218000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3747 bp

ATGCAGCAGCATCAACCTCCGCCAGTCCCACTGCAATCGCAGACCGCCGGAACCCCAGCCGGAGTGCAGGTCCGCTGCGCCGGCTGCGGCAAGATCCTGACTTTGGACGCAGGGGTCACCGAATTCGCCTGCGACGCCTGCCAATTGCCCCAAATGTTGCCGCCCGAGCTGATGACCCGAGCCCAGGCATATGCCCAGGCCCGCGCTCCGCCTCCGCCCCATGTTGCGCCTCACGGCGTTGATCCTTCGAAGATCCAGCTTCCCTGCGCTAATTGCAAGGCCATCCTCAACGTGCCTCATGGCCTCGCGAGGTTCCGGTGCCCTCAGTGCCAAGTTGACCTGGCTGTCGATGTCTCGAAGCTCCAGCAGTTCTTCCCGCCCCGTCTGCCCCTGCCTCCTCCACCCGAGGAGGTCAATGAGGTCGCCATCGAAGTAGAGCGAGAAGAGGATGAAGGTGGCAAGGCAGGCGAAACATTTACAGACTATCGCCCTCCAAAACTATCCATTGGCCCTCCTCATCCAGATCCTGTTGTGGAAACATCGTCCTTGGCTGCTGTGCAGCCACCTGAGCCCACTTATGATCTGAAAATCAAGGATGATTTGGAAAATTTAAAGGCTTTGTCATGCTTACAGATTGAGACATTGGTCTATGCTTGTCAGAGACACCTGCAGCATCTTCCAAGTGGTGAGAGGGCAGGATTCTTTGTTGGGGATGGAGCTGGTGTGGGGAAAGGTCGAACAATAGCAGGGTTAATATGGGAGAATTGGCACCGTGGGATGAGGAAAGCAGTGTGGGTTTCTGTTGGTTCAGACTTGAAGTTTGATGCGAGAAGAGACTTAGATGATGTAGGTGCAACTTGCATTGAAGTACACGCCTTGAACAAGCTGCCTTATTCCAAACTTGATTCGAAGTCTGTTGGCGTCAAGGAGGGCGTTGTTTTTCTGACATACAGCAGCCTCATTGCATCCTCTGAGAAAGGTCGTTCACGTATGGGGCAGCTTGTGCAGTGGTGTGGACCAGGATTTAATGGTCTTGTTGTATTTGATGAGTGCCACAAGGCCAAAAATCTGGTGCCTGAATCTGGAAGTCAGCCAACACGTACTGGTGAAGCAGTTCTTGAAATTCAGGCTAAGCTTCCTGAAGCTCGTGTTATTTATTGTTCAGCAACTGGTGCATCCGAACCACGAAATTTGGGTTACATGGTACGCCTTGGTCTTTGGGGCCCTGGAACATCTTTTTCTGATTTCCGTGAATTTCTAGGTGCTCTTGAGAAAGGAGGCGTTGGAGCACTCGAACTTGTTGCCATGGATATGAAAGCAAGGGGGATGTACGTATGCCGTACTCTTAGCTACAAAGGGGCAGAGTTTGAAGTTGTTGAAGCACCGCTAGAAGCTGAAATGATGGACATGTACGGAAAAGCAGCTGAATTTTGGACAGAACTGCGACAGGATATACTAACTGCATCTGAATTTCTTGCTAATGAAAAGCCTGTTTCTAGTCAAGTGTGGCGATTATATTGGGCCAGCCATCAGCGTTTCTTTAGACACATGTGCATGTCAGCTAAGGTGCCGGCTGCTGTAAGACTAGCTAAGCAAGCATTGATGGATGGCAAGTGTGTGGTTATTGGCCTTCAGAGTACTGGAGAGGCACGAACTGAGGAGGCTGTGACCAAATATGGTCTTGAACTTGATGATTTTATTTCCGGACCTCGAGAACTTTTACTGAAATTTGTTGAGGAAAATTATCCCTTGCCTGAAAAGCCTGAACCTCTCGAAGGAGAAGATAGTGTGAAGGAGCTGCAGCGGAAGAGGCACTCAGCAACTCCTGGTGTTTCAATGAAAGGGAGAGTGAGGAAAGTTGCAAAATGGAAACCTGCAAGTGATGATGAAAGTGATGAAGAATCTGAAACTGAGTCTGCTCAGGAATCTACTGAATCTGACGATGAGTTTCAAATTTGTGAAATTTGCCAATCTGAGGAGGAAAGAAAGAAACTGCTTCATTGTTCCTGTTGTGGGCAACTGGTCCATGCTGCATGTCTTATCCCACCTGTGACAGATGAAGTATCAGTAGACTGGTCATGCCATTCATGCAAGGAAAAAACAGATGAATTTTTGAAAAGAAGACAAGAGTATATAGCTGTACTTACGAAAAGGTATGAAGAAGCTTTGGCTCGTAAGTTAAACATATTGGAGATAGTTCGTTCTTTGGATCTTCCGAACAATCCTTTGGATGATATCATTGATCAGCTTGGAGGCCCTGATAAAGTAGCAGAAATGACTGGAAGGCGAGGTATGCTTGTGAGGGCATCTGGTGGAAAAGGTGTTACCTATCAGGCACGGAACACGAAGGAAATTTCCATGGAGATGGTAAACATGCATGAGAAGCAGCTTTTCATGGACGGCAAGAAGTTGGTTGCCATCATTTCTGAAGCTGGATCTGCTGGTGTGTCTTTGCAGGCAGATAGAAGAGCTACAAATCAGAGACGAAGGGTACATCTTACTCTGGAACTTCCTTGGAGTGCTGATCGAGCAATTCAACAGTTTGGAAGAACTCATCGGTCAAATCAAGCCTCCGCTCCTGAATACAGGCTTATTTTTACTAACCTTGGTGGAGAGCGTCGATTTGCATCCATCGTAGCAAAGAGATTAGAATCTCTTGGAGCACTAACACAGGGTGACCGCAGGGCAGGGCTATCATTGAGTGCCTATAACTACGATAGTGCTTATGGAAAGAAGGCCTTGATGTTGATGTATAGAGGAATCATGGAGCAGGATTCTCTGCCTGTTGTGCCGCTAGGATGTTCATCAGAAAAACCGGAAACAATCCAAGATTTTATTGAGAAGGCTAAAGCTGCTCTTGTTCTTGTAGGAATAGTTAGGGATGCTCATGGGAAAGATCATGGCAGGCTCGCTGGCCGTATAATTGAATCAGATATGCATGATGTTGGACGTTTCCTCAATCGGATTTTAGGAGTACCGCCTGATATACAGAATAGGCTGTTTGAGTGTTTCGTTAGCATTTTGGATCATATTGTCCATAATGCACGCATTGAAGGGAATCTGGACTCTGGGATTGTTGATATGAAAGCTAATGTTATAGAGCTACAAGGAACTCCAAAGACTGTTTACGTAGATCAAATGTCTGGAGCTTCGACAGTGCTTTTTACATTTACCCTGGATCGTGGGGTCACGTGGGAGTCTGCGAGTGCCATGCTTGAAGAGAAACAAAAGGATGGACTTGGTTCTGCCAATGATGGATTCTATGAATCTAGGAGGGAATGGTTGGGAAGACGTCATGTCATGTTAGCCTTTGAGAGTTCTACTTCCGGATTGAACAAGATAGTCCGCCCTGCGGTGGGAGAGTCAGTAAGAGAAATGCCTCTGTCAGAGCTAAAAAGCAAATACAGAAAAACATCAACATTAGAGAAGGCTCGTCGTGGCTGGGAAGATGAATATGAAGTTTCGTCCAAACAGTGCATGCATGGACGCAATTGTAAGCTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGCCTAATTCTTCCTGTCTGGGGCACTATTGAGAAGGCTCTTTCTAAGCAGCAACGGCAAAGCCACAAGCGGCTACGTGTTGTGCGTATAGAAACCACTACTGATAACAGCAGAATCGTCGGTCTCTCTGTACCAAATGCAGCAGTGGAATCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATTGACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1249

Amino Acids

138.31

Weight (kDa)

6.21

Isoelectric Point (pI)

51.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_FGT1_1	PF23547	22 - 56	4.6e-18	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1	PF23547	85 - 114	4.8e-10	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2	PF23548	85 - 118	6.5e-17	FORGETTER1 second zinc ribbon domain
AAA_34	PF13872	172 - 472	1.3e-132	P-loop containing NTP hydrolase pore-1
PHD	PF00628	653 - 697	5.7e-06	PHD-finger
Helicase_C_4	PF13871	741 - 1027	7.2e-106	C-terminal domain on Strawberry notch homologue
SBNO	PF25373	1064 - 1176	2e-17	SBNO alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006161)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79350
fragaria_vesca	FvH4_3g13920 FvH4_3g13920 FvH4_3g13920 FvH4_3g13920
malus_domestica	MD05G1239900.v1.1 MD10G1218000.v1.1
prunus_persica	Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1
pyrus_communis	pycom05g21600 pycom10g18380
rosa_chinensis	RchiOBHm_Chr5g0023151
rosa_laevigata	RLG00000032703
rosa_multiflora	Rmu_co8366291.1_g000001 Rmu_sc0007300.1_g000019 Rmu_sc0015109.1_g000002 Rmu_sc0033377.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056190
rosa_rugosa	Rorug05G0077600
rosa_samantha	Rh5AG163600 Rh5BG163000 Rh5CG177800 Rh5DG164200
rosa_wichuraiana	Rw5G014760 Rw5G015070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	675
AasI	GACNNNNNNGTC	2 cut(s)	113, 344
AatII	GACGTC	1 cut(s)	3295
Acc36I	ACCTGC	3 cut(s)	58, 675, 1882
AccB1I	GGYRCC	4 cut(s)	318, 768, 1072, 1567
AccBSI	CCGCTC	1 cut(s)	2576
AccI	GTMKAC	1 cut(s)	2070
AccII	CGCG	2 cut(s)	204, 308
AccIII	TCCGGA	2 cut(s)	1703, 3326
AclWI	GGATC	8 cut(s)	88, 236, 247, 518, 2217, 2443, 3033, 3186
AcoI	YGGCCR	1 cut(s)	2914
AcuI	CTGAAG	3 cut(s)	1161, 1616, 2448
AcvI	CACGTG	1 cut(s)	3192
AcyI	GRCGYC	3 cut(s)	135, 921, 3292
AdeI	CACNNNGTG	1 cut(s)	2675
AflIII	ACRYGT	4 cut(s)	1097, 1407, 1548, 3628
AhdI	GACNNNNNGTC	1 cut(s)	3296
AjnI	CCWGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
AjuI	GAANNNNNNNTTGG	4 cut(s)	2210, 2242, 3242, 3274
Alw21I	GWGCWC	3 cut(s)	1267, 1291, 2665
Alw26I	GTCTC	6 cut(s)	358, 632, 655, 828, 2476, 3680
AlwI	GGATC	8 cut(s)	88, 236, 247, 518, 2217, 2443, 3033, 3186
AlwNI	CAGNNNCTG	2 cut(s)	527, 1169
Ama87I	CYCGRG	4 cut(s)	164, 177, 404, 1710
Aor13HI	TCCGGA	2 cut(s)	1703, 3326
ApaI	GGGCCC	1 cut(s)	1226
ArsI	GACNNNNNNTTYG	2 cut(s)	803, 835
Asp700I	GAANNNNTTC	1 cut(s)	1113
AspLEI	GCGC	4 cut(s)	80, 206, 229, 269
AsuHPI	GGTGA	4 cut(s)	109, 698, 1118, 2687
AsuII	TTCGAA	2 cut(s)	248, 908
AvaI	CYCGRG	4 cut(s)	164, 177, 404, 1710
AvaII	GGWCC	4 cut(s)	70, 1016, 1706, 2023
BaeGI	GKGCMC	3 cut(s)	323, 1226, 3578
BanI	GGYRCC	4 cut(s)	318, 768, 1072, 1567
BanII	GRGCYC	3 cut(s)	184, 573, 1226
BauI	CACGAG	1 cut(s)	1146
BbrPI	CACGTG	1 cut(s)	3192
BbsI	GAAGAC	3 cut(s)	2128, 3295, 3532
Bbv12I	GWGCWC	3 cut(s)	1267, 1291, 2665
BceAI	ACGGC	5 cut(s)	250, 1323, 2416, 2901, 3623
BcgI	CGANNNNNNTGC	6 cut(s)	1283, 1317, 3193, 3227, 3652, 3686
BciT130I	CCWGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
BclI	TGATCA	1 cut(s)	2242
BcoDI	GTCTC	6 cut(s)	358, 632, 655, 828, 2476, 3680
BfaI	CTAG	6 cut(s)	1259, 1388, 1497, 1586, 2795, 3270
BfmI	CTRYAG	3 cut(s)	668, 1799, 3530
BfuAI	ACCTGC	3 cut(s)	58, 675, 1882
BlpI	GCTNAGC	2 cut(s)	1131, 1590
BmcAI	AGTACT	1 cut(s)	1639
Bme1390I	CCNGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
Bme18I	GGWCC	4 cut(s)	70, 1016, 1706, 2023
BmeRI	GACNNNNNGTC	1 cut(s)	3296
BmeT110I	CYCGRG	4 cut(s)	164, 177, 404, 1710
BmiI	GGNNCC	8 cut(s)	52, 314, 320, 770, 1074, 1223, 1224, 1569
BmrFI	CCNGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
BmsI	GCATC	9 cut(s)	19, 682, 814, 974, 1184, 1472, 2315, 2636, 2872
BoxI	GACNNNNGTC	1 cut(s)	644
BpiI	GAAGAC	3 cut(s)	2128, 3295, 3532
BpmI	CTGGAG	3 cut(s)	347, 1662, 3165
Bpu10I	CCTNAGC	3 cut(s)	567, 1563, 1920
Bpu1102I	GCTNAGC	2 cut(s)	1131, 1590
Bpu14I	TTCGAA	2 cut(s)	248, 908
BpuEI	CTTGAG	1 cut(s)	1289
BsaAI	YACGTR	6 cut(s)	989, 1100, 1333, 3130, 3192, 3629
BsaBI	GATNNNNATC	3 cut(s)	2626, 2650, 2803
BsaHI	GRCGYC	3 cut(s)	135, 921, 3292
BsaI	GGTCTC	1 cut(s)	3680
BsaWI	WCCGGW	4 cut(s)	315, 1703, 2815, 3326
BsaXI	ACNNNNNCTCC	6 cut(s)	54, 84, 708, 738, 3351, 3381
Bse118I	RCCGGY	2 cut(s)	80, 1570
Bse1I	ACTGG	7 cut(s)	23, 1108, 1174, 1645, 2025, 2078, 2284
Bse3DI	GCAATG	1 cut(s)	960
Bse8I	GATNNNNATC	3 cut(s)	2626, 2650, 2803
BseAI	TCCGGA	2 cut(s)	1703, 3326
BseBI	CCWGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
BseJI	GATNNNNATC	3 cut(s)	2626, 2650, 2803
BseMI	GCAATG	1 cut(s)	960
BseMII	CTCAG	8 cut(s)	338, 558, 963, 1647, 1830, 1902, 1934, 1965
BseNI	ACTGG	7 cut(s)	23, 1108, 1174, 1645, 2025, 2078, 2284
BseRI	GAGGAG	5 cut(s)	387, 422, 504, 1673, 1991
BseSI	GKGCMC	3 cut(s)	323, 1226, 3578
BseYI	CCCAGC	2 cut(s)	55, 3441
BsgI	GTGCAG	3 cut(s)	86, 578, 1025
Bsh1236I	CGCG	2 cut(s)	204, 308
BshNI	GGYRCC	4 cut(s)	318, 768, 1072, 1567
BsiHKAI	GWGCWC	3 cut(s)	1267, 1291, 2665
BsiHKCI	CYCGRG	4 cut(s)	164, 177, 404, 1710
BsiSI	CCGG	8 cut(s)	48, 60, 81, 316, 1571, 1704, 2816, 3327
BslFI	GGGAC	1 cut(s)	11
BsmAI	GTCTC	6 cut(s)	358, 632, 655, 828, 2476, 3680
BsmBI	CGTCTC	1 cut(s)	2476
BsmFI	GGGAC	1 cut(s)	11
Bso31I	GGTCTC	1 cut(s)	3680
BsoBI	CYCGRG	4 cut(s)	164, 177, 404, 1710
Bsp119I	TTCGAA	2 cut(s)	248, 908
Bsp120I	GGGCCC	1 cut(s)	1222
Bsp1286I	GDGCHC	8 cut(s)	184, 323, 573, 1226, 1267, 1291, 2665, 3578
Bsp13I	TCCGGA	2 cut(s)	1703, 3326
Bsp1720I	GCTNAGC	2 cut(s)	1131, 1590
Bsp19I	CCATGG	2 cut(s)	1305, 2358
Bsp68I	TCGCGA	1 cut(s)	308
BspCNI	CTCAG	8 cut(s)	337, 559, 964, 1648, 1829, 1903, 1933, 1966
BspEI	TCCGGA	2 cut(s)	1703, 3326
BspFNI	CGCG	2 cut(s)	204, 308
BspLI	GGNNCC	8 cut(s)	52, 314, 320, 770, 1074, 1223, 1224, 1569
BspMAI	CTGCAG	3 cut(s)	672, 1803, 3534
BspMI	ACCTGC	3 cut(s)	58, 675, 1882
BspPI	GGATC	8 cut(s)	88, 236, 247, 518, 2217, 2443, 3033, 3186
BspQI	GCTCTTC	1 cut(s)	2461
BspT104I	TTCGAA	2 cut(s)	248, 908
BspT107I	GGYRCC	4 cut(s)	318, 768, 1072, 1567
BspTNI	GGTCTC	1 cut(s)	3680
BsrBI	CCGCTC	1 cut(s)	2576
BsrDI	GCAATG	1 cut(s)	960
BsrFI	RCCGGY	2 cut(s)	80, 1570
BsrI	ACTGG	7 cut(s)	23, 1108, 1174, 1645, 2025, 2078, 2284
BssAI	RCCGGY	2 cut(s)	80, 1570
BssNI	GRCGYC	3 cut(s)	135, 921, 3292
BssSI	CACGAG	1 cut(s)	1146
BssT1I	CCWWGG	6 cut(s)	546, 1210, 1305, 2358, 2513, 2605
Bst2BI	CACGAG	1 cut(s)	1146
Bst2UI	CCWGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
Bst4CI	ACNGT	6 cut(s)	773, 2541, 3124, 3157, 3474, 3517
Bst6I	CTCTTC	5 cut(s)	438, 826, 1802, 2461, 3213
BstACI	GRCGYC	3 cut(s)	135, 921, 3292
BstAPI	GCANNNNNTGC	2 cut(s)	1003, 1172
BstBAI	YACGTR	6 cut(s)	989, 1100, 1333, 3130, 3192, 3629
BstBI	TTCGAA	2 cut(s)	248, 908
BstDSI	CCRYGG	3 cut(s)	772, 1305, 2358
BstEII	GGTNACC	2 cut(s)	115, 2675
BstENI	CCTNNNNNAGG	1 cut(s)	1773
BstFNI	CGCG	2 cut(s)	204, 308
BstHHI	GCGC	4 cut(s)	80, 206, 229, 269
BstMAI	GTCTC	6 cut(s)	358, 632, 655, 828, 2476, 3680
BstNI	CCWGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
BstNSI	RCATGY	6 cut(s)	1411, 1552, 1558, 2037, 2377, 3481
BstPAI	GACNNNNGTC	1 cut(s)	644
BstPI	GGTNACC	2 cut(s)	115, 2675
BstSCI	CCNGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
BstSFI	CTRYAG	3 cut(s)	668, 1799, 3530
BstSLI	GKGCMC	3 cut(s)	323, 1226, 3578
BstSNI	TACGTA	2 cut(s)	1333, 3130
BstUI	CGCG	2 cut(s)	204, 308
BstV2I	GAAGAC	3 cut(s)	2128, 3295, 3532
BstX2I	RGATCY	5 cut(s)	93, 252, 523, 2209, 2435
BstXI	CCANNNNNNTGG	2 cut(s)	1069, 2365
BstYI	RGATCY	5 cut(s)	93, 252, 523, 2209, 2435
BtgI	CCRYGG	3 cut(s)	772, 1305, 2358
BtsI	GCAGTG	4 cut(s)	29, 795, 1013, 3702
BtsIMutI	CAGTG	7 cut(s)	29, 332, 795, 1013, 3162, 3479, 3702
BtuMI	TCGCGA	1 cut(s)	308
BveI	ACCTGC	3 cut(s)	58, 675, 1882
CaiI	CAGNNNCTG	2 cut(s)	527, 1169
CfoI	GCGC	4 cut(s)	80, 206, 229, 269
Cfr10I	RCCGGY	2 cut(s)	80, 1570
CseI	GACGC	5 cut(s)	116, 143, 910, 2606, 3495
CspCI	CAANNNNNGTGG	2 cut(s)	1173, 1208
DraI	TTTAAA	1 cut(s)	612
DraIII	CACNNNGTG	1 cut(s)	2675
DrdI	GACNNNNNNGTC	2 cut(s)	113, 344
DriI	GACNNNNNGTC	1 cut(s)	3296
DseDI	GACNNNNNNGTC	2 cut(s)	113, 344
EaeI	YGGCCR	1 cut(s)	2914
Eam1104I	CTCTTC	5 cut(s)	438, 826, 1802, 2461, 3213
Eam1105I	GACNNNNNGTC	1 cut(s)	3296
EarI	CTCTTC	5 cut(s)	438, 826, 1802, 2461, 3213
EciI	GGCGGA	4 cut(s)	9, 198, 204, 3335
Eco105I	TACGTA	2 cut(s)	1333, 3130
Eco130I	CCWWGG	6 cut(s)	546, 1210, 1305, 2358, 2513, 2605
Eco147I	AGGCCT	1 cut(s)	2738
Eco24I	GRGCYC	3 cut(s)	184, 573, 1226
Eco31I	GGTCTC	1 cut(s)	3680
Eco32I	GATATC	1 cut(s)	2236
Eco47I	GGWCC	4 cut(s)	70, 1016, 1706, 2023
Eco57I	CTGAAG	3 cut(s)	1161, 1616, 2448
Eco72I	CACGTG	1 cut(s)	3192
Eco88I	CYCGRG	4 cut(s)	164, 177, 404, 1710
Eco91I	GGTNACC	2 cut(s)	115, 2675
EcoNI	CCTNNNNNAGG	1 cut(s)	1773
EcoO109I	RGGNCCY	3 cut(s)	1222, 1223, 2255
EcoO65I	GGTNACC	2 cut(s)	115, 2675
EcoRI	GAATTC	1 cut(s)	122
EcoRII	CCWGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
EcoRV	GATATC	1 cut(s)	2236
EcoT14I	CCWWGG	6 cut(s)	546, 1210, 1305, 2358, 2513, 2605
EcoT22I	ATGCAT	3 cut(s)	2379, 2940, 3483
EcoT38I	GRGCYC	3 cut(s)	184, 573, 1226
ErhI	CCWWGG	6 cut(s)	546, 1210, 1305, 2358, 2513, 2605
Esp3I	CGTCTC	1 cut(s)	2476
FalI	AAGNNNNNCTT	6 cut(s)	1101, 1133, 2723, 2755, 2841, 2873
FaqI	GGGAC	1 cut(s)	11
FauI	CCCGC	2 cut(s)	209, 384
FauNDI	CATATG	1 cut(s)	190
FbaI	TGATCA	1 cut(s)	2242
FblI	GTMKAC	1 cut(s)	2070
FriOI	GRGCYC	3 cut(s)	184, 573, 1226
FspBI	CTAG	6 cut(s)	1259, 1388, 1497, 1586, 2795, 3270
GlaI	GCGC	4 cut(s)	79, 205, 228, 268
GsaI	CCCAGC	2 cut(s)	59, 3445
GsuI	CTGGAG	3 cut(s)	347, 1662, 3165
HapII	CCGG	8 cut(s)	48, 60, 81, 316, 1571, 1704, 2816, 3327
HgaI	GACGC	5 cut(s)	116, 143, 910, 2606, 3495
HhaI	GCGC	4 cut(s)	80, 206, 229, 269
Hin1I	GRCGYC	3 cut(s)	135, 921, 3292
Hin6I	GCGC	4 cut(s)	78, 204, 227, 267
HinP1I	GCGC	4 cut(s)	78, 204, 227, 267
HincII	GTYRAC	1 cut(s)	337
HindII	GTYRAC	1 cut(s)	337
HindIII	AAGCTT	3 cut(s)	1133, 2163, 3497
HpaII	CCGG	8 cut(s)	48, 60, 81, 316, 1571, 1704, 2816, 3327
HphI	GGTGA	4 cut(s)	109, 698, 1118, 2687
Hpy166II	GTNNAC	7 cut(s)	337, 871, 986, 1016, 2071, 2371, 3127
Hpy8I	GTNNAC	7 cut(s)	337, 871, 986, 1016, 2071, 2371, 3127
Hpy99I	CGWCG	4 cut(s)	137, 2622, 3438, 3674
HpyCH4III	ACNGT	6 cut(s)	773, 2541, 3124, 3157, 3474, 3517
HpyCH4IV	ACGT	9 cut(s)	291, 988, 1099, 1332, 2950, 3129, 3191, 3292, 3628
HpySE526I	ACGT	9 cut(s)	291, 988, 1099, 1332, 2950, 3129, 3191, 3292, 3628
Hsp92I	GRCGYC	3 cut(s)	135, 921, 3292
HspAI	GCGC	4 cut(s)	78, 204, 227, 267
Kpn2I	TCCGGA	2 cut(s)	1703, 3326
KroI	GCCGGC	2 cut(s)	80, 1570
KroNI	GCCGGC	2 cut(s)	82, 1572
Ksp22I	TGATCA	1 cut(s)	2242
LguI	GCTCTTC	1 cut(s)	2461
LmnI	GCTCC	9 cut(s)	211, 366, 716, 1286, 1795, 2581, 2660, 2767, 3146
LweI	GCATC	9 cut(s)	19, 682, 814, 974, 1184, 1472, 2315, 2636, 2872
MaeI	CTAG	6 cut(s)	1259, 1388, 1497, 1586, 2795, 3270
MaeII	ACGT	9 cut(s)	291, 988, 1099, 1332, 2950, 3129, 3191, 3292, 3628
MaeIII	GTNAC	7 cut(s)	115, 1196, 1666, 2050, 2323, 2675, 3187
MbiI	CCGCTC	1 cut(s)	2576
MfeI	CAATTG	2 cut(s)	143, 3490
MflI	RGATCY	5 cut(s)	93, 252, 523, 2209, 2435
MhlI	GDGCHC	8 cut(s)	184, 323, 573, 1226, 1267, 1291, 2665, 3578
MlyI	GAGTC	4 cut(s)	1922, 3059, 3206, 3371
MmeI	TCCRAC	2 cut(s)	1264, 2926
Mph1103I	ATGCAT	3 cut(s)	2379, 2940, 3483
MroI	TCCGGA	2 cut(s)	1703, 3326
MroNI	GCCGGC	2 cut(s)	80, 1570
MroXI	GAANNNNTTC	1 cut(s)	1113
MseI	TTAA	4 cut(s)	611, 752, 1026, 2180
MslI	CAYNNNNRTG	3 cut(s)	1310, 1553, 2363
MspA1I	CMGCKG	3 cut(s)	75, 1424, 1804
MspI	CCGG	8 cut(s)	48, 60, 81, 316, 1571, 1704, 2816, 3327
MspR9I	CCNGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
MunI	CAATTG	2 cut(s)	143, 3490
MvaI	CCWGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
MvnI	CGCG	2 cut(s)	204, 308
NaeI	GCCGGC	2 cut(s)	82, 1572
NcoI	CCATGG	2 cut(s)	1305, 2358
NdeI	CATATG	1 cut(s)	190
NgoMIV	GCCGGC	2 cut(s)	80, 1570
NlaIV	GGNNCC	8 cut(s)	52, 314, 320, 770, 1074, 1223, 1224, 1569
NmuCI	GTSAC	5 cut(s)	115, 1666, 2050, 2675, 3187
NruI	TCGCGA	1 cut(s)	308
NsiI	ATGCAT	3 cut(s)	2379, 2940, 3483
NspI	RCATGY	6 cut(s)	1411, 1552, 1558, 2037, 2377, 3481
NspV	TTCGAA	2 cut(s)	248, 908
PaeI	GCATGC	1 cut(s)	3481
PaeR7I	CTCGAG	1 cut(s)	1710
PaqCI	CACCTGC	1 cut(s)	675
PceI	AGGCCT	1 cut(s)	2738
PciI	ACATGT	2 cut(s)	1407, 1548
PciSI	GCTCTTC	1 cut(s)	2461
PcsI	WCGNNNNNNNCGW	1 cut(s)	3634
PdiI	GCCGGC	2 cut(s)	82, 1572
PdmI	GAANNNNTTC	1 cut(s)	1113
PleI	GAGTC	4 cut(s)	1921, 3059, 3205, 3370
PmaCI	CACGTG	1 cut(s)	3192
PmlI	CACGTG	1 cut(s)	3192
PpsI	GAGTC	4 cut(s)	1921, 3059, 3205, 3370
Ppu21I	YACGTR	6 cut(s)	989, 1100, 1333, 3130, 3192, 3629
PscI	ACATGT	2 cut(s)	1407, 1548
PshAI	GACNNNNGTC	1 cut(s)	644
Psp6I	CCWGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
PspCI	CACGTG	1 cut(s)	3192
PspEI	GGTNACC	2 cut(s)	115, 2675
PspFI	CCCAGC	2 cut(s)	55, 3441
PspGI	CCWGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
PspN4I	GGNNCC	8 cut(s)	52, 314, 320, 770, 1074, 1223, 1224, 1569
PspOMI	GGGCCC	1 cut(s)	1222
PstI	CTGCAG	3 cut(s)	672, 1803, 3534
PstNI	CAGNNNCTG	2 cut(s)	527, 1169
PsuI	RGATCY	5 cut(s)	93, 252, 523, 2209, 2435
PvuII	CAGCTG	1 cut(s)	1424
RruI	TCGCGA	1 cut(s)	308
RseI	CAYNNNNRTG	3 cut(s)	1310, 1553, 2363
SapI	GCTCTTC	1 cut(s)	2461
SaqAI	TTAA	4 cut(s)	611, 752, 1026, 2180
ScaI	AGTACT	1 cut(s)	1639
SchI	GAGTC	4 cut(s)	1922, 3059, 3206, 3371
ScrFI	CCNGG	7 cut(s)	185, 197, 341, 1020, 1227, 1827, 3176
SduI	GDGCHC	8 cut(s)	184, 323, 573, 1226, 1267, 1291, 2665, 3578
SfaNI	GCATC	9 cut(s)	19, 682, 814, 974, 1184, 1472, 2315, 2636, 2872
SfcI	CTRYAG	3 cut(s)	668, 1799, 3530
Sfr274I	CTCGAG	1 cut(s)	1710
SfuI	TTCGAA	2 cut(s)	248, 908
SinI	GGWCC	4 cut(s)	70, 1016, 1706, 2023
SlaI	CTCGAG	1 cut(s)	1710
SmiMI	CAYNNNNRTG	3 cut(s)	1310, 1553, 2363
SmlI	CTYRAG	2 cut(s)	1268, 1710
SmoI	CTYRAG	2 cut(s)	1268, 1710
SnaBI	TACGTA	2 cut(s)	1333, 3130
SphI	GCATGC	1 cut(s)	3481
SseBI	AGGCCT	1 cut(s)	2738
SspMI	CTAG	6 cut(s)	1259, 1388, 1497, 1586, 2795, 3270
StuI	AGGCCT	1 cut(s)	2738
StyD4I	CCNGG	7 cut(s)	183, 195, 339, 1018, 1225, 1825, 3174
StyI	CCWWGG	6 cut(s)	546, 1210, 1305, 2358, 2513, 2605
TaaI	ACNGT	6 cut(s)	773, 2541, 3124, 3157, 3474, 3517
TaiI	ACGT	9 cut(s)	294, 991, 1102, 1335, 2953, 3132, 3194, 3295, 3631
TaqII	GACCGA	2 cut(s)	2547, 3662
TatI	WGTACW	4 cut(s)	868, 1637, 2140, 3512
TauI	GCSGC	4 cut(s)	90, 163, 2794, 3625
Tru1I	TTAA	4 cut(s)	611, 752, 1026, 2180
Tru9I	TTAA	4 cut(s)	611, 752, 1026, 2180
TscAI	CASTG	7 cut(s)	36, 332, 795, 1013, 3162, 3479, 3702
TseFI	GTSAC	5 cut(s)	115, 1666, 2050, 2675, 3187
Tsp45I	GTSAC	5 cut(s)	115, 1666, 2050, 2675, 3187
TspGWI	ACGGA	4 cut(s)	1238, 1428, 2353, 3694
TspRI	CASTG	7 cut(s)	36, 332, 795, 1013, 3162, 3479, 3702
VpaK11BI	GGWCC	4 cut(s)	70, 1016, 1706, 2023
XagI	CCTNNNNNAGG	1 cut(s)	1773
XceI	RCATGY	6 cut(s)	1411, 1552, 1558, 2037, 2377, 3481
XhoI	CTCGAG	1 cut(s)	1710
XmiI	GTMKAC	1 cut(s)	2070
XmnI	GAANNNNTTC	1 cut(s)	1113
XspI	CTAG	6 cut(s)	1259, 1388, 1497, 1586, 2795, 3270
ZraI	GACGTC	1 cut(s)	3293
ZrmI	AGTACT	1 cut(s)	1639
Zsp2I	ATGCAT	3 cut(s)	2379, 2940, 3483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.