Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0006161

Physical Location & Seq

Genomic Coordinates Reverse (-)

16970649 .. 16985052

Gene Structure 14404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG163000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3777 bp

ATGGCGCAGCAGCAGCAGCAGCCTCCGTCGGTCCCGCCGCAATTGCCGACCGCCGGCGCCAGCGGGCCCGTCGGAGGCGTCCAGGTGCGGTGCGCCGGCTGCCACAAGATCCTCACCATCGCGGCCGGGGTCACCGAATTCGTCTGCGGCACGTGTCAATTGCCGCAGATGCTTCCGCCGGAGCTGATGAGCCGGGCCCAGGCCCATAACCAGCCCAACAAGGGCCCGCTTCCCCTGCCCCCGCCGCGGCCGCAGCCGCAGCACGTGGCCGCCCACGGCGTCGATCCTTCGAAGATCCAGCTCCCCTGCGCCAACTGCAAGGCGATTCTCAATGTGCCTCATGGACTCTCTCGGTTTCAGTGCCCTCAGTGCCATGTTGACCTCGCCGTCGATGTGTCCAAGCTCAAAGAGTTCTTGCTGCCACCACTGCCTTCCGAGGAGGTCAACGAGGTTGCCATTGAAGTGGAGCGAGAAGAAGATGAAGGTGGCCTGGCCGGGGAAACATTTACAGATTATCGCCCTCCAAAGCTGTCCATTGGACCTCCTCATCCAGATCCCGTTGTGGAAACATCATCCCTGTCGGCTGTGCAGCCACCTGAGCCCACTTACGACCTAACAATCAAGGATGATTTGGAAAATTCAAAGGCTTTATCATGTTTGCAGATTGAGACATTGGTTTATGCCTGTCAGAGACACCTCCAGCACCTTCCAAGTGGTGTTAGGGCTGGATTCTTTGTTGGAGATGGAGCTGGTGTGGGTAAAGGTCGAACAATTGCAGGGTTAATTTGGGAAAATTGGCACCATGGGAGGAGAAAGGCAGTGTGGATTTCTGTTGGTTCAGACTTGAAGTTTGATGCAAGAAGAGACTTAGATGATGTAGGCGCAACTTGTATAGAAGTTCATGCTTTGAACAAGCTGCCATATTCTAAGCTTGATTCCAAGTCTGTTGGCATCAAGGAGGGAGTTATATTCCTCACATACAGCAGCCTTATAGCATCTTCTGAGAAAGGTCGTTCACGCATGCAGCAGCTTGTGCAGTGGTGTGGATCAGGATATGATGGTCTTATCGTCTTTGACGAGTGTCACAAAGCCAAAAATTTGATACCTGAAGCTGGAAGTCAGCCAACACGAACCGGTGAAGCAGTTCTTGACATTCAGGCTCGACTGCCTGAAGCTCGGGTTATTTATTGCTCGGCAACTGGTGCATCAGAACCACGCAACTTGGGTTACATGGTTCGGCTTGGTCTTTGGGGCCCTGGAACATCTTTTTCTGAGTTCCGTGAATTTCTAGGTGCTCTTGAGAAAGGAGGTGTTGGTGCACTTGAACTTGTTGCCATGGACATGAAAGCAAGGGGGATGTATGTCTGTCGTACTCTTAGCTATAAAGGAGCAGAGTTTGACGTTGTTGAGGCACCACTAGAAGATGAAATGATGGAAATGTACAAAAAAGCAGCAGAATTTTGGACAGAGCTGCGAGTTGAAATACTAACAGCTACTGCCTATCTCACTAATGAAAAGCCCATTTCTAGTCAAGTCTGGCGATTGTATTGGGCTAGTCATCAGCGTTTCTTTAGACATATGTGTATGTCGGCTAAGGTTCCTGCTGCTGTAAGACTAGCGAAGAAAGCATTGATGGATGACAAGTGTGTCGTAATTGGCCTTCAGAGTACTGGAGAGGCACGAACTGAAGAAGCTGTGACCAAATATGGTCTTGAGCTTGATGATTTTATTTCGGGACCTCGAGAACTGTTGCTGAAATTTGTTGAAGAAAACTATCCTTTGCCTGAAAAGCCTGATCCTGTCACAGGAGAAGAAAGTGTAAAGGAGCTTCAACGGAAGAGGCACTCAGCAAGTCCTGGTGTTTCGATGAAAGGGAGAGTGAGGAAAGTTGCAAAAGTGCAACCGCCAATTGATAACGAAAGTGACGAAGAATCTGAAACTGATTCTGCTGTTGAATCTACTGAATCAGATGACGAGTTTCAAATCTGTGAAATCTGCAATGCCGAAACGGAAAGAAAGAAATTGCTCCACTGTTCTTGTTGTGGCCAACTGGTCCATGCTGAATGCCTTATCCCACCTGTGATAGATGAGGTATCAGAAGATTGGTCATGTCATTCGTGCAAGGAAAAAACAGATGAATATCTTCAACGGAGAGAGCAGTATATAGCTGAATTGAAGAAGAGGTATGAAGCAGCTTTGGAGCGTAAGTCAAAAATTCTGGGGATAATTCGTTCCTTGGATCTTCCAAACAATCCTTTGGACGATATCATTGATCAGCTTGGAGGCCCTGATAAAGTAGCTGAAATGACAGGAAGGCGAGGTATGCTTGTGAGGGCATCTAATGGTAAAGGTGTTACCTATCAGGCTCGAAACACGAAGGAGGTTTCCATGGAGATGGTCAACATGCATGAGAAACAGCTTTTCATGGATGGCAAAAAGTTTGTTGCTATAATTTCTGAGGCTGGGTCAGCTGGTGTTTCTTTGCAGGCAGATAGAAGAGCTGTAAATCAGAAAAGAAGGGTACATCTAACCCTGGAACTTCCTTGGAGTGCCGATCGAGCGATTCAACAGTTTGGAAGAACTCACCGGTCAAATCAAGCTTCTGCTCCTGAATACAGGCTACTCTTTACTAACCTCGGTGGAGAACGTCGGTTTGCATCCATTGTTGCAAAGAGATTACAATCTCTTGGAGCTCTAACTCAGGGTGATCGCAGGGCAGGCCCATCATTGAGTGCCTATAATTATGATAGTGCTTACGGAAAGAAGGCATTAATATTGATGTATAGAGGAATATTGGAGCAGGAAGCTCTGCCTGTTGTACCACCAGGATGTTCATCTGAAGACCCGGAGACAATCCAAGATTTTATTGAGGAGGCAAGAGCTGCTCTTGTGTTTGTAGGAATAATTAGGGATGCTAAAGATTCTGGCAAGCTCTCTGGCCGTGTAGCTGATTCAGACATGCATGACGTCGGACGTTTCCTCAATCGGATTTTAGGATTGCCACCTAAAGTACAAAATAGGCTGTTCGAGTTATTTGTTAGTATTTTGGATCTTCTTGTCCATAATGCACGCATTGAAGGAAATCTTGACTCTGGGATTGTTGATATGAAAGCTAATGTTATAGAGCTACAAGGAACTCCAAAGACTGTCCATGTTGACGAAATGTCTGGAGCTTCAACTGTGCTCTTCACTTTTACCCTGGATCGTGGCATCACATGGGAGTCTGCAAGTGCCATGCTTGAAGGTAAACAAGAGGAGGGGCTTAGTTGTGCTCATGATGGCTTCTATGAATCTAGAAGGGAGTGGATGGGGAGGCGCCATACCATATTAGCCTTTGAAAGTTCTACTTCTGGGTCCTATAAGATAGTCCGTCCGGCAGTTGGAGAGTCGGTGAGAGAAATGTCTCTGTCTGAGCTAAAAAGCAAATACAGAAGAACATCATCGTTGGAGAAGGCTCGCAGCGGTTGGAATGATGAATATGAAGTTTCATCCAAACAGTGCATGCATGGTCCCAAGTGTAAGGTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGTCTCATTCTTCCTGTATGGGGCACTATTGAGAAAGCTCTTTCTAAGCAGTCCCGGCAAAGCCACAGGCGGCTACGTGTTGTGCGTATAGAAACCACAACAGATAACCAGAGAATCGTTGGTCTGTTTGTCCCCAATGCAGCTGTTGAAGCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATTGAAGATTCACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1258

Amino Acids

139.28

Weight (kDa)

6.15

Isoelectric Point (pI)

52.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_FGT1_1	PF23547	27 - 61	3.5e-18	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1	PF23547	99 - 128	6.4e-10	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2	PF23548	99 - 132	3.8e-17	FORGETTER1 second zinc ribbon domain
AAA_34	PF13872	182 - 482	1.7e-134	P-loop containing NTP hydrolase pore-1
Helicase_C_4	PF13871	751 - 1035	2.5e-104	C-terminal domain on Strawberry notch homologue
SBNO	PF25373	1072 - 1184	2.6e-18	SBNO alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006161)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79350
fragaria_vesca	FvH4_3g13920 FvH4_3g13920 FvH4_3g13920 FvH4_3g13920
malus_domestica	MD05G1239900.v1.1 MD10G1218000.v1.1
prunus_persica	Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1
pyrus_communis	pycom05g21600 pycom10g18380
rosa_chinensis	RchiOBHm_Chr5g0023151
rosa_laevigata	RLG00000032703
rosa_multiflora	Rmu_co8366291.1_g000001 Rmu_sc0007300.1_g000019 Rmu_sc0015109.1_g000002 Rmu_sc0033377.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056190
rosa_rugosa	Rorug05G0077600
rosa_samantha	Rh5AG163600 Rh5BG163000 Rh5CG177800 Rh5DG164200
rosa_wichuraiana	Rw5G014760 Rw5G015070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	386, 1646
AatII	GACGTC	1 cut(s)	2970
AccB1I	GGYRCC	4 cut(s)	56, 798, 1411, 3315
AccII	CGCG	2 cut(s)	122, 247
AclWI	GGATC	9 cut(s)	103, 278, 289, 548, 1054, 1790, 2247, 3057, 3210
AcoI	YGGCCR	6 cut(s)	123, 248, 267, 492, 2044, 2938
AcsI	RAATTY	7 cut(s)	137, 637, 1096, 1283, 1457, 1757, 2214
AcuI	CTGAAG	5 cut(s)	1128, 1191, 1646, 1707, 2859
AcvI	CACGTG	2 cut(s)	153, 265
AcyI	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
AfaI	GTAC	8 cut(s)	1372, 1442, 1669, 2523, 2820, 3012, 3538, 3732
AflIII	ACRYGT	2 cut(s)	152, 3652
AgeI	ACCGGT	2 cut(s)	1133, 2586
AhdI	GACNNNNNGTC	1 cut(s)	3163
AjnI	CCWGG	8 cut(s)	81, 198, 489, 1255, 1855, 2532, 2824, 3198
AloI	GAACNNNNNNTCC	2 cut(s)	2215, 2247
Alw21I	GWGCWC	5 cut(s)	1297, 1321, 2695, 3186, 3274
Alw26I	GTCTC	6 cut(s)	662, 685, 858, 2843, 3408, 3584
Alw44I	GTGCAC	1 cut(s)	1317
AlwI	GGATC	9 cut(s)	103, 278, 289, 548, 1054, 1790, 2247, 3057, 3210
AlwNI	CAGNNNCTG	1 cut(s)	1496
Ama87I	CYCGRG	2 cut(s)	1176, 1740
ApaI	GGGCCC	4 cut(s)	69, 199, 227, 1256
ApaLI	GTGCAC	1 cut(s)	1317
ApoI	RAATTY	7 cut(s)	137, 637, 1096, 1283, 1457, 1757, 2214
ArsI	GACNNNNNNTTYG	2 cut(s)	833, 865
AseI	ATTAAT	1 cut(s)	2771
AsiGI	ACCGGT	2 cut(s)	1133, 2586
Asp700I	GAANNNNTTC	2 cut(s)	1143, 2142
AspLEI	GCGC	6 cut(s)	7, 59, 95, 311, 884, 3318
AsuC2I	CCSGG	5 cut(s)	127, 194, 496, 2846, 3631
AsuHPI	GGTGA	6 cut(s)	106, 124, 1148, 2576, 2717, 3403
AsuII	TTCGAA	1 cut(s)	290
AvaI	CYCGRG	2 cut(s)	1176, 1740
AvaII	GGWCC	6 cut(s)	31, 539, 1736, 2053, 3354, 3509
BaeGI	GKGCMC	7 cut(s)	69, 199, 227, 365, 1256, 1321, 3602
BalI	TGGCCA	1 cut(s)	2046
BanI	GGYRCC	4 cut(s)	56, 798, 1411, 3315
BanII	GRGCYC	6 cut(s)	69, 199, 227, 603, 1256, 2695
BarI	GAAGNNNNNNTAC	4 cut(s)	1644, 1676, 1812, 1844
BbrPI	CACGTG	2 cut(s)	153, 265
BbsI	GAAGAC	2 cut(s)	2847, 3556
Bbv12I	GWGCWC	5 cut(s)	1297, 1321, 2695, 3186, 3274
BceAI	ACGGC	4 cut(s)	292, 371, 2925, 3713
BciT130I	CCWGG	8 cut(s)	83, 200, 491, 1257, 1857, 2534, 2826, 3200
BclI	TGATCA	1 cut(s)	2272
BcnI	CCSGG	5 cut(s)	127, 194, 496, 2846, 3631
BcoDI	GTCTC	6 cut(s)	662, 685, 858, 2843, 3408, 3584
BfaI	CTAG	6 cut(s)	1289, 1418, 1527, 1554, 1616, 3294
BfmI	CTRYAG	1 cut(s)	3554
BfoI	RGCGCY	2 cut(s)	60, 3319
BmcAI	AGTACT	1 cut(s)	1669
Bme18I	GGWCC	6 cut(s)	31, 539, 1736, 2053, 3354, 3509
BmeRI	GACNNNNNGTC	1 cut(s)	3163
BmeT110I	CYCGRG	2 cut(s)	1176, 1740
BmsI	GCATC	9 cut(s)	159, 844, 960, 1004, 1214, 2345, 2666, 2902, 3219
BoxI	GACNNNNGTC	1 cut(s)	1080
BpiI	GAAGAC	2 cut(s)	2847, 3556
BpmI	CTGGAG	3 cut(s)	683, 1692, 3189
Bpu10I	CCTNAGC	2 cut(s)	597, 1593
Bpu14I	TTCGAA	1 cut(s)	290
BpuEI	CTTGAG	2 cut(s)	1319, 1733
BpuMI	CCSGG	5 cut(s)	127, 194, 496, 2846, 3631
BsaAI	YACGTR	3 cut(s)	153, 265, 3653
BsaBI	GATNNNNATC	3 cut(s)	2139, 2680, 2833
BsaHI	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
BsaI	GGTCTC	1 cut(s)	3584
BsaWI	WCCGGW	2 cut(s)	1133, 2586
BsaXI	ACNNNNNCTCC	6 cut(s)	738, 768, 2192, 2222, 3375, 3405
Bse118I	RCCGGY	4 cut(s)	53, 95, 1133, 2586
Bse1I	ACTGG	3 cut(s)	1204, 1675, 2055
Bse3DI	GCAATG	1 cut(s)	2005
Bse8I	GATNNNNATC	3 cut(s)	2139, 2680, 2833
BseBI	CCWGG	8 cut(s)	83, 200, 491, 1257, 1857, 2534, 2826, 3200
BseJI	GATNNNNATC	3 cut(s)	2139, 2680, 2833
BseMI	GCAATG	1 cut(s)	2005
BseMII	CTCAG	8 cut(s)	380, 588, 993, 1263, 1860, 2448, 2714, 3402
BseNI	ACTGG	3 cut(s)	1204, 1675, 2055
BseRI	GAGGAG	5 cut(s)	452, 534, 823, 2885, 3269
BseSI	GKGCMC	7 cut(s)	69, 199, 227, 365, 1256, 1321, 3602
BseX3I	CGGCCG	2 cut(s)	123, 248
BseYI	CCCAGC	1 cut(s)	2462
BsgI	GTGCAG	2 cut(s)	608, 1055
Bsh1236I	CGCG	2 cut(s)	122, 247
Bsh1285I	CGRYCG	4 cut(s)	51, 126, 251, 2557
BshNI	GGYRCC	4 cut(s)	56, 798, 1411, 3315
BshTI	ACCGGT	2 cut(s)	1133, 2586
BsiEI	CGRYCG	4 cut(s)	51, 126, 251, 2557
BsiHKAI	GWGCWC	5 cut(s)	1297, 1321, 2695, 3186, 3274
BsiHKCI	CYCGRG	2 cut(s)	1176, 1740
BslFI	GGGAC	5 cut(s)	17, 1749, 3495, 3613, 3692
BsmAI	GTCTC	6 cut(s)	662, 685, 858, 2843, 3408, 3584
BsmFI	GGGAC	5 cut(s)	17, 1749, 3495, 3613, 3692
BsmI	GAATGC	1 cut(s)	2069
Bso31I	GGTCTC	1 cut(s)	3584
BsoBI	CYCGRG	2 cut(s)	1176, 1740
Bsp119I	TTCGAA	1 cut(s)	290
Bsp120I	GGGCCC	4 cut(s)	65, 195, 223, 1252
Bsp1407I	TGTACA	1 cut(s)	1440
Bsp19I	CCATGG	3 cut(s)	802, 1335, 2388
BspCNI	CTCAG	8 cut(s)	379, 589, 994, 1264, 1859, 2449, 2713, 3403
BspFNI	CGCG	2 cut(s)	122, 247
BspHI	TCATGA	1 cut(s)	3274
BspMAI	CTGCAG	1 cut(s)	3558
BspPI	GGATC	9 cut(s)	103, 278, 289, 548, 1054, 1790, 2247, 3057, 3210
BspQI	GCTCTTC	2 cut(s)	2491, 3191
BspT104I	TTCGAA	1 cut(s)	290
BspT107I	GGYRCC	4 cut(s)	56, 798, 1411, 3315
BspTNI	GGTCTC	1 cut(s)	3584
BsrDI	GCAATG	1 cut(s)	2005
BsrFI	RCCGGY	4 cut(s)	53, 95, 1133, 2586
BsrGI	TGTACA	1 cut(s)	1440
BsrI	ACTGG	3 cut(s)	1204, 1675, 2055
BssAI	RCCGGY	4 cut(s)	53, 95, 1133, 2586
BssNI	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
BssT1I	CCWWGG	5 cut(s)	802, 1335, 2235, 2388, 2543
Bst2UI	CCWGG	8 cut(s)	83, 200, 491, 1257, 1857, 2534, 2826, 3200
Bst4CI	ACNGT	8 cut(s)	1749, 2033, 2571, 3148, 3181, 3498, 3541, 3774
Bst6I	CTCTTC	5 cut(s)	856, 1832, 2174, 2491, 3191
BstACI	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
BstAPI	GCANNNNNTGC	3 cut(s)	1033, 1202, 2882
BstAUI	TGTACA	1 cut(s)	1440
BstBAI	YACGTR	3 cut(s)	153, 265, 3653
BstBI	TTCGAA	1 cut(s)	290
BstDSI	CCRYGG	5 cut(s)	245, 274, 802, 1335, 2388
BstEII	GGTNACC	1 cut(s)	130
BstENI	CCTNNNNNAGG	1 cut(s)	1803
BstFNI	CGCG	2 cut(s)	122, 247
BstH2I	RGCGCY	2 cut(s)	60, 3319
BstHHI	GCGC	6 cut(s)	7, 59, 95, 311, 884, 3318
BstMAI	GTCTC	6 cut(s)	662, 685, 858, 2843, 3408, 3584
BstMCI	CGRYCG	4 cut(s)	51, 126, 251, 2557
BstNI	CCWGG	8 cut(s)	83, 200, 491, 1257, 1857, 2534, 2826, 3200
BstNSI	RCATGY	4 cut(s)	1024, 2407, 2962, 3505
BstPAI	GACNNNNGTC	1 cut(s)	1080
BstPI	GGTNACC	1 cut(s)	130
BstSFI	CTRYAG	1 cut(s)	3554
BstSLI	GKGCMC	7 cut(s)	69, 199, 227, 365, 1256, 1321, 3602
BstUI	CGCG	2 cut(s)	122, 247
BstV2I	GAAGAC	2 cut(s)	2847, 3556
BstX2I	RGATCY	5 cut(s)	108, 294, 553, 2239, 3049
BstXI	CCANNNNNNTGG	2 cut(s)	463, 2395
BstYI	RGATCY	5 cut(s)	108, 294, 553, 2239, 3049
BstZI	CGGCCG	2 cut(s)	123, 248
BtgI	CCRYGG	5 cut(s)	245, 274, 802, 1335, 2388
BtgZI	GCGATG	1 cut(s)	103
BtsI	GCAGTG	3 cut(s)	425, 825, 1043
BtsIMutI	CAGTG	7 cut(s)	365, 374, 425, 825, 1043, 2029, 3503
CaiI	CAGNNNCTG	1 cut(s)	1496
CciI	TCATGA	1 cut(s)	3274
CciNI	GCGGCCGC	1 cut(s)	248
CfoI	GCGC	6 cut(s)	7, 59, 95, 311, 884, 3318
Cfr10I	RCCGGY	4 cut(s)	53, 95, 1133, 2586
Cfr42I	CCGCGG	1 cut(s)	248
CseI	GACGC	2 cut(s)	67, 268
Csp6I	GTAC	8 cut(s)	1371, 1441, 1668, 2522, 2819, 3011, 3537, 3731
CspAI	ACCGGT	2 cut(s)	1133, 2586
CspCI	CAANNNNNGTGG	4 cut(s)	1203, 1238, 2018, 2053
CviQI	GTAC	8 cut(s)	1371, 1441, 1668, 2522, 2819, 3011, 3537, 3731
DinI	GGCGCC	2 cut(s)	58, 3317
DrdI	GACNNNNNNGTC	2 cut(s)	386, 1646
DriI	GACNNNNNGTC	1 cut(s)	3163
DseDI	GACNNNNNNGTC	2 cut(s)	386, 1646
EaeI	YGGCCR	6 cut(s)	123, 248, 267, 492, 2044, 2938
EagI	CGGCCG	2 cut(s)	123, 248
Eam1104I	CTCTTC	5 cut(s)	856, 1832, 2174, 2491, 3191
Eam1105I	GACNNNNNGTC	1 cut(s)	3163
EarI	CTCTTC	5 cut(s)	856, 1832, 2174, 2491, 3191
EciI	GGCGGA	1 cut(s)	165
Ecl136II	GAGCTC	1 cut(s)	2693
EclXI	CGGCCG	2 cut(s)	123, 248
Eco130I	CCWWGG	5 cut(s)	802, 1335, 2235, 2388, 2543
Eco24I	GRGCYC	6 cut(s)	69, 199, 227, 603, 1256, 2695
Eco31I	GGTCTC	1 cut(s)	3584
Eco32I	GATATC	1 cut(s)	2266
Eco47I	GGWCC	6 cut(s)	31, 539, 1736, 2053, 3354, 3509
Eco52I	CGGCCG	2 cut(s)	123, 248
Eco53kI	GAGCTC	1 cut(s)	2693
Eco57I	CTGAAG	5 cut(s)	1128, 1191, 1646, 1707, 2859
Eco72I	CACGTG	2 cut(s)	153, 265
Eco88I	CYCGRG	2 cut(s)	1176, 1740
Eco91I	GGTNACC	1 cut(s)	130
EcoICRI	GAGCTC	1 cut(s)	2693
EcoNI	CCTNNNNNAGG	1 cut(s)	1803
EcoO109I	RGGNCCY	6 cut(s)	223, 1252, 1253, 1736, 2285, 3354
EcoO65I	GGTNACC	1 cut(s)	130
EcoRI	GAATTC	1 cut(s)	137
EcoRII	CCWGG	8 cut(s)	81, 198, 489, 1255, 1855, 2532, 2824, 3198
EcoRV	GATATC	1 cut(s)	2266
EcoT14I	CCWWGG	5 cut(s)	802, 1335, 2235, 2388, 2543
EcoT22I	ATGCAT	3 cut(s)	2409, 2964, 3507
EcoT38I	GRGCYC	6 cut(s)	69, 199, 227, 603, 1256, 2695
EgeI	GGCGCC	2 cut(s)	58, 3317
EheI	GGCGCC	2 cut(s)	58, 3317
ErhI	CCWWGG	5 cut(s)	802, 1335, 2235, 2388, 2543
FalI	AAGNNNNNCTT	8 cut(s)	1131, 1163, 3069, 3101, 3331, 3363, 3717, 3749
FaqI	GGGAC	5 cut(s)	17, 1749, 3495, 3613, 3692
FauI	CCCGC	4 cut(s)	42, 56, 234, 249
FauNDI	CATATG	1 cut(s)	1578
FbaI	TGATCA	1 cut(s)	2272
FriOI	GRGCYC	6 cut(s)	69, 199, 227, 603, 1256, 2695
FspBI	CTAG	6 cut(s)	1289, 1418, 1527, 1554, 1616, 3294
GlaI	GCGC	6 cut(s)	6, 58, 94, 310, 883, 3317
GsaI	CCCAGC	1 cut(s)	2466
GsuI	CTGGAG	3 cut(s)	683, 1692, 3189
HaeII	RGCGCY	2 cut(s)	60, 3319
HgaI	GACGC	2 cut(s)	67, 268
HhaI	GCGC	6 cut(s)	7, 59, 95, 311, 884, 3318
Hin1I	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
Hin6I	GCGC	6 cut(s)	5, 57, 93, 309, 882, 3316
HinP1I	GCGC	6 cut(s)	5, 57, 93, 309, 882, 3316
HincII	GTYRAC	4 cut(s)	379, 445, 2401, 3157
HindII	GTYRAC	4 cut(s)	379, 445, 2401, 3157
HindIII	AAGCTT	2 cut(s)	929, 2598
HphI	GGTGA	6 cut(s)	106, 124, 1148, 2576, 2717, 3403
Hpy166II	GTNNAC	7 cut(s)	379, 445, 1016, 1319, 2401, 3157, 3248
Hpy8I	GTNNAC	7 cut(s)	379, 445, 1016, 1319, 2401, 3157, 3248
Hpy99I	CGWCG	6 cut(s)	31, 74, 284, 392, 2652, 2972
HpyCH4III	ACNGT	8 cut(s)	1749, 2033, 2571, 3148, 3181, 3498, 3541, 3774
HpyCH4IV	ACGT	7 cut(s)	152, 264, 1401, 2647, 2967, 2974, 3652
HpySE526I	ACGT	7 cut(s)	152, 264, 1401, 2647, 2967, 2974, 3652
Hsp92I	GRCGYC	5 cut(s)	57, 78, 279, 2967, 3316
HspAI	GCGC	6 cut(s)	5, 57, 93, 309, 882, 3316
KasI	GGCGCC	2 cut(s)	56, 3315
KroI	GCCGGC	2 cut(s)	53, 95
KroNI	GCCGGC	2 cut(s)	55, 97
Ksp22I	TGATCA	1 cut(s)	2272
KspI	CCGCGG	1 cut(s)	248
LguI	GCTCTTC	2 cut(s)	2491, 3191
LweI	GCATC	9 cut(s)	159, 844, 960, 1004, 1214, 2345, 2666, 2902, 3219
MaeI	CTAG	6 cut(s)	1289, 1418, 1527, 1554, 1616, 3294
MaeII	ACGT	7 cut(s)	152, 264, 1401, 2647, 2967, 2974, 3652
MaeIII	GTNAC	7 cut(s)	130, 1082, 1226, 1696, 1801, 1922, 2353
MfeI	CAATTG	4 cut(s)	41, 158, 771, 1908
MflI	RGATCY	5 cut(s)	108, 294, 553, 2239, 3049
MlsI	TGGCCA	1 cut(s)	2046
MluNI	TGGCCA	1 cut(s)	2046
Mly113I	GGCGCC	2 cut(s)	57, 3316
MlyI	GAGTC	4 cut(s)	339, 3083, 3230, 3395
MmeI	TCCRAC	6 cut(s)	52, 718, 2950, 3361, 3426, 3446
Mox20I	TGGCCA	1 cut(s)	2046
Mph1103I	ATGCAT	3 cut(s)	2409, 2964, 3507
MreI	CGCCGGCG	1 cut(s)	53
MroNI	GCCGGC	2 cut(s)	53, 95
MroXI	GAANNNNTTC	2 cut(s)	1143, 2142
MscI	TGGCCA	1 cut(s)	2046
MseI	TTAA	2 cut(s)	782, 2771
MslI	CAYNNNNRTG	4 cut(s)	461, 1340, 2393, 2827
Msp20I	TGGCCA	1 cut(s)	2046
MspA1I	CMGCKG	5 cut(s)	63, 247, 2471, 3462, 3719
MunI	CAATTG	4 cut(s)	41, 158, 771, 1908
Mva1269I	GAATGC	1 cut(s)	2069
MvaI	CCWGG	8 cut(s)	83, 200, 491, 1257, 1857, 2534, 2826, 3200
MvnI	CGCG	2 cut(s)	122, 247
NaeI	GCCGGC	2 cut(s)	55, 97
NarI	GGCGCC	2 cut(s)	57, 3316
NciI	CCSGG	5 cut(s)	127, 194, 496, 2846, 3631
NcoI	CCATGG	3 cut(s)	802, 1335, 2388
NdeI	CATATG	1 cut(s)	1578
NgoMIV	GCCGGC	2 cut(s)	53, 95
NmeAIII	GCCGAG	1 cut(s)	1172
NmuCI	GTSAC	5 cut(s)	130, 1082, 1696, 1801, 1922
NotI	GCGGCCGC	1 cut(s)	248
NsiI	ATGCAT	3 cut(s)	2409, 2964, 3507
NspI	RCATGY	4 cut(s)	1024, 2407, 2962, 3505
NspV	TTCGAA	1 cut(s)	290
PaeI	GCATGC	2 cut(s)	1024, 3505
PaeR7I	CTCGAG	1 cut(s)	1740
PagI	TCATGA	1 cut(s)	3274
PciSI	GCTCTTC	2 cut(s)	2491, 3191
PcsI	WCGNNNNNNNCGW	3 cut(s)	1074, 1923, 3658
PctI	GAATGC	1 cut(s)	2069
PdiI	GCCGGC	2 cut(s)	55, 97
PdmI	GAANNNNTTC	2 cut(s)	1143, 2142
PinAI	ACCGGT	2 cut(s)	1133, 2586
Ple19I	CGATCG	1 cut(s)	2557
PleI	GAGTC	4 cut(s)	339, 3083, 3229, 3394
PluTI	GGCGCC	2 cut(s)	60, 3319
PmaCI	CACGTG	2 cut(s)	153, 265
PmlI	CACGTG	2 cut(s)	153, 265
PpsI	GAGTC	4 cut(s)	339, 3083, 3229, 3394
Ppu21I	YACGTR	3 cut(s)	153, 265, 3653
PpuMI	RGGWCCY	2 cut(s)	1736, 3354
PshAI	GACNNNNGTC	1 cut(s)	1080
PshBI	ATTAAT	1 cut(s)	2771
Psp124BI	GAGCTC	1 cut(s)	2695
Psp5II	RGGWCCY	2 cut(s)	1736, 3354
Psp6I	CCWGG	8 cut(s)	81, 198, 489, 1255, 1855, 2532, 2824, 3198
PspCI	CACGTG	2 cut(s)	153, 265
PspEI	GGTNACC	1 cut(s)	130
PspFI	CCCAGC	1 cut(s)	2462
PspGI	CCWGG	8 cut(s)	81, 198, 489, 1255, 1855, 2532, 2824, 3198
PspOMI	GGGCCC	4 cut(s)	65, 195, 223, 1252
PspPPI	RGGWCCY	2 cut(s)	1736, 3354
PstI	CTGCAG	1 cut(s)	3558
PstNI	CAGNNNCTG	1 cut(s)	1496
PsuI	RGATCY	5 cut(s)	108, 294, 553, 2239, 3049
PvuI	CGATCG	1 cut(s)	2557
PvuII	CAGCTG	2 cut(s)	2471, 3719
RsaI	GTAC	8 cut(s)	1372, 1442, 1669, 2523, 2820, 3012, 3538, 3732
RsaNI	GTAC	8 cut(s)	1371, 1441, 1668, 2522, 2819, 3011, 3537, 3731
RseI	CAYNNNNRTG	4 cut(s)	461, 1340, 2393, 2827
SacI	GAGCTC	1 cut(s)	2695
SacII	CCGCGG	1 cut(s)	248
SapI	GCTCTTC	2 cut(s)	2491, 3191
SaqAI	TTAA	2 cut(s)	782, 2771
ScaI	AGTACT	1 cut(s)	1669
SchI	GAGTC	4 cut(s)	339, 3083, 3230, 3395
SfaNI	GCATC	9 cut(s)	159, 844, 960, 1004, 1214, 2345, 2666, 2902, 3219
SfcI	CTRYAG	1 cut(s)	3554
SfoI	GGCGCC	2 cut(s)	58, 3317
Sfr274I	CTCGAG	1 cut(s)	1740
Sfr303I	CCGCGG	1 cut(s)	248
SfuI	TTCGAA	1 cut(s)	290
SgrAI	CRCCGGYG	1 cut(s)	53
SgrBI	CCGCGG	1 cut(s)	248
SinI	GGWCC	6 cut(s)	31, 539, 1736, 2053, 3354, 3509
SlaI	CTCGAG	1 cut(s)	1740
SmiMI	CAYNNNNRTG	4 cut(s)	461, 1340, 2393, 2827
SmlI	CTYRAG	3 cut(s)	1298, 1712, 1740
SmoI	CTYRAG	3 cut(s)	1298, 1712, 1740
SphI	GCATGC	2 cut(s)	1024, 3505
SspDI	GGCGCC	2 cut(s)	56, 3315
SspI	AATATT	2 cut(s)	2775, 2793
SspMI	CTAG	6 cut(s)	1289, 1418, 1527, 1554, 1616, 3294
SstI	GAGCTC	1 cut(s)	2695
StyI	CCWWGG	5 cut(s)	802, 1335, 2235, 2388, 2543
TaaI	ACNGT	8 cut(s)	1749, 2033, 2571, 3148, 3181, 3498, 3541, 3774
TaiI	ACGT	7 cut(s)	155, 267, 1404, 2650, 2970, 2977, 3655
TaqII	GACCGA	1 cut(s)	19
TatI	WGTACW	4 cut(s)	1440, 1667, 3010, 3536
Tru1I	TTAA	2 cut(s)	782, 2771
Tru9I	TTAA	2 cut(s)	782, 2771
TscAI	CASTG	7 cut(s)	365, 374, 432, 825, 1043, 2036, 3503
TseFI	GTSAC	5 cut(s)	130, 1082, 1696, 1801, 1922
Tsp45I	GTSAC	5 cut(s)	130, 1082, 1696, 1801, 1922
TspGWI	ACGGA	7 cut(s)	15, 1268, 1849, 2024, 2164, 2772, 3359
TspRI	CASTG	7 cut(s)	365, 374, 432, 825, 1043, 2036, 3503
VneI	GTGCAC	1 cut(s)	1317
VpaK11BI	GGWCC	6 cut(s)	31, 539, 1736, 2053, 3354, 3509
VspI	ATTAAT	1 cut(s)	2771
XagI	CCTNNNNNAGG	1 cut(s)	1803
XapI	RAATTY	7 cut(s)	137, 637, 1096, 1283, 1457, 1757, 2214
XbaI	TCTAGA	1 cut(s)	3293
XceI	RCATGY	4 cut(s)	1024, 2407, 2962, 3505
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3692
XhoI	CTCGAG	1 cut(s)	1740
XmnI	GAANNNNTTC	2 cut(s)	1143, 2142
XspI	CTAG	6 cut(s)	1289, 1418, 1527, 1554, 1616, 3294
ZraI	GACGTC	1 cut(s)	2968
ZrmI	AGTACT	1 cut(s)	1669
Zsp2I	ATGCAT	3 cut(s)	2409, 2964, 3507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.