MD05G1246200.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008334
Physical Location & Seq
Reverse (-)
37763796 .. 37766710
2915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1246200.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGAGGCCATTGGAAATGCAGGGACTGCAATTGGGATATTGTCGAAAGATGGAGTTGTGTTGGTAGGTGAAAAGAAGGTGACTTCCAAGCTCCTTCAGACCTCTACATCAACAGAGAAGATGTACAAGATTGATGACCATGTTGCATGTGCCGTGGCTGGGATAATGTCTGATGCCAACATCCTGATCAACACAGCCAGGGTTCAAGCACAGCGCTACACATTTGCTTACCAAGAGCCAATGCCAGTTGAGCAACTAGTCCAGTCTCTCTGTGACACAAAACAAGGCTACACGCAGTTTGGTGGGCTCCGTCCATTTGGCGTTTCGTTTCTGTTTGCAGGTTGGGACAAAAACTACGGCTTTCAGCTGTACATGAGCGACCCAAGTGGAAACTATGGCGGCTGGAAGGCTGCAGCCATTGGAGCGAACAACCAGGCAGCACAGTCAATGCTGAAGCAGGACTACAAGGATGAAATAACAAGGGAAGAGGCGGTTGAGCTTGCACTGAAGGTGCTCAGCAAGACAATGGACAGCACTAGTCTGACATCCGACAAGCTTGAGTTAGCGGAAGTCTTTGTCTTGCCTTCGGGTAAAGTGAAGTACCAGGTGTGCTCGCCAGATGCGCTGAGCAAACTGTTGGTGAAGAGTGGAGTGACTCAACCTGCCGCCGAGGCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

227

Amino Acids

24.37

Weight (kDa)

5.54

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 6 - 190 2.1e-60 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 329, 670
AciI CCGC 4 cut(s) 399, 491, 566, 666
AcuI CTGAAG 3 cut(s) 80, 473, 527
AfaI GTAC 3 cut(s) 125, 371, 602
AfeI AGCGCT 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 1 cut(s) 206
AhlI ACTAGT 2 cut(s) 256, 536
AjnI CCWGG 3 cut(s) 197, 432, 603
AluBI AGCT 4 cut(s) 91, 367, 499, 556
AluI AGCT 4 cut(s) 91, 367, 499, 556
Alw21I GWGCWC 2 cut(s) 516, 614
Alw26I GTCTC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 26
Aor51HI AGCGCT 1 cut(s) 215
AoxI GGCC 2 cut(s) 6, 672
ApeKI GCWGC 3 cut(s) 410, 413, 437
AspLEI GCGC 2 cut(s) 216, 625
AsuHPI GGTGA 3 cut(s) 80, 91, 652
BanII GRGCYC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 446, 478
Bbv12I GWGCWC 2 cut(s) 516, 614
BbvI GCAGC 3 cut(s) 397, 425, 449
BccI CCATC 1 cut(s) 44
BceAI ACGGC 2 cut(s) 137, 373
BciT130I CCWGG 3 cut(s) 199, 434, 605
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 2 cut(s) 256, 536
BfaI CTAG 2 cut(s) 257, 537
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 217
BfuAI ACCTGC 2 cut(s) 329, 670
BglI GCCNNNNNGGC 1 cut(s) 671
BisI GCNGC 5 cut(s) 400, 411, 414, 438, 666
BlpI GCTNAGC 2 cut(s) 515, 626
BlsI GCNGC 5 cut(s) 401, 412, 415, 439, 667
Bme1390I CCNGG 3 cut(s) 199, 434, 605
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 3 cut(s) 199, 434, 605
BmsI GCATC 2 cut(s) 163, 610
Bpu1102I GCTNAGC 2 cut(s) 515, 626
BpuEI CTTGAG 1 cut(s) 578
BsaJI CCNNGG 3 cut(s) 153, 198, 669
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse1I ACTGG 2 cut(s) 245, 262
BseBI CCWGG 3 cut(s) 199, 434, 605
BseDI CCNNGG 3 cut(s) 153, 198, 669
BseGI GGATG 3 cut(s) 180, 475, 545
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 529, 617
BseNI ACTGG 2 cut(s) 245, 262
BseXI GCAGC 3 cut(s) 397, 425, 449
BseYI CCCAGC 1 cut(s) 158
BshFI GGCC 2 cut(s) 8, 674
BsiHKAI GWGCWC 2 cut(s) 516, 614
BslFI GGGAC 2 cut(s) 37, 359
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 2 cut(s) 37, 359
BsnI GGCC 2 cut(s) 8, 674
Bsp1286I GDGCHC 3 cut(s) 309, 516, 614
Bsp1407I TGTACA 2 cut(s) 123, 369
Bsp143I GATC 1 cut(s) 186
Bsp1720I GCTNAGC 2 cut(s) 515, 626
BspACI CCGC 4 cut(s) 399, 491, 566, 666
BspANI GGCC 2 cut(s) 8, 674
BspCNI CTCAG 2 cut(s) 528, 618
BspLI GGNNCC 1 cut(s) 308
BspMAI CTGCAG 1 cut(s) 415
BspMI ACCTGC 2 cut(s) 329, 670
BsrGI TGTACA 2 cut(s) 123, 369
BsrI ACTGG 2 cut(s) 245, 262
BssECI CCNNGG 3 cut(s) 153, 198, 669
BssMI GATC 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 199, 434, 605
Bst4CI ACNGT 2 cut(s) 444, 636
Bst6I CTCTTC 2 cut(s) 480, 638
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 2 cut(s) 123, 369
BstC8I GCNNGC 2 cut(s) 501, 614
BstDEI CTNAG 3 cut(s) 515, 626, 678
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 180, 475, 545
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 2 cut(s) 216, 625
BstKTI GATC 1 cut(s) 189
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 5 cut(s) 26, 250, 422, 622, 671
BstNI CCWGG 3 cut(s) 199, 434, 605
BstNSI RCATGY 1 cut(s) 150
BstSCI CCNGG 3 cut(s) 197, 432, 603
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 397, 425, 449
BsuRI GGCC 2 cut(s) 8, 674
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 180, 475, 545
BtsIMutI CAGTG 1 cut(s) 503
BveI ACCTGC 2 cut(s) 329, 670
Cac8I GCNNGC 2 cut(s) 501, 614
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 2 cut(s) 216, 625
CsiI ACCWGGT 1 cut(s) 603
Csp6I GTAC 3 cut(s) 124, 370, 601
CviAII CATG 3 cut(s) 140, 147, 373
CviQI GTAC 3 cut(s) 124, 370, 601
DdeI CTNAG 3 cut(s) 515, 626, 678
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 480, 638
EarI CTCTTC 2 cut(s) 480, 638
Eco147I AGGCCT 1 cut(s) 674
Eco24I GRGCYC 1 cut(s) 309
Eco47III AGCGCT 1 cut(s) 215
Eco57I CTGAAG 3 cut(s) 80, 473, 527
EcoRII CCWGG 3 cut(s) 197, 432, 603
EcoT38I GRGCYC 1 cut(s) 309
FaeI CATG 3 cut(s) 143, 150, 376
FaiI YATR 4 cut(s) 141, 148, 374, 396
FaqI GGGAC 2 cut(s) 37, 359
FatI CATG 3 cut(s) 139, 146, 372
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 400, 411, 414, 438, 666
FokI GGATG 3 cut(s) 167, 482, 532
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 400, 411, 414, 438, 666
FspBI CTAG 2 cut(s) 257, 537
GlaI GCGC 2 cut(s) 215, 624
GluI GCNGC 5 cut(s) 400, 411, 414, 438, 666
GsaI CCCAGC 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 2 cut(s) 8, 674
HhaI GCGC 2 cut(s) 216, 625
Hin1II CATG 3 cut(s) 143, 150, 376
Hin6I GCGC 2 cut(s) 214, 623
HinP1I GCGC 2 cut(s) 214, 623
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 1 cut(s) 655
HphI GGTGA 3 cut(s) 80, 91, 652
Hpy188I TCNGA 4 cut(s) 99, 172, 543, 550
Hpy188III TCNNGA 1 cut(s) 184
HpyAV CCTTC 5 cut(s) 70, 104, 400, 502, 594
HpyCH4III ACNGT 2 cut(s) 444, 636
HpyCH4V TGCA 6 cut(s) 20, 29, 146, 338, 413, 503
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 250, 422, 622, 671
HpyF3I CTNAG 3 cut(s) 515, 626, 678
Hsp92II CATG 3 cut(s) 143, 150, 376
HspAI GCGC 2 cut(s) 214, 623
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 96, 312, 422
Lsp1109I GCAGC 3 cut(s) 397, 425, 449
LweI GCATC 2 cut(s) 163, 610
MabI ACCWGGT 1 cut(s) 603
MaeI CTAG 2 cut(s) 257, 537
MaeIII GTNAC 3 cut(s) 79, 272, 652
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 130, 497, 655
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 3 cut(s) 309, 516, 614
MluCI AATT 1 cut(s) 30
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 573
MnlI CCTC 3 cut(s) 112, 481, 664
MspA1I CMGCKG 1 cut(s) 367
MspR9I CCNGG 3 cut(s) 199, 434, 605
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 3 cut(s) 199, 434, 605
MwoI GCNNNNNNNGC 5 cut(s) 26, 250, 422, 622, 671
NdeII GATC 1 cut(s) 186
NlaIII CATG 3 cut(s) 143, 150, 376
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 3 cut(s) 79, 272, 652
NspI RCATGY 1 cut(s) 150
PceI AGGCCT 1 cut(s) 674
PkrI GCNGC 5 cut(s) 401, 412, 415, 439, 667
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Psp6I CCWGG 3 cut(s) 197, 432, 603
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 3 cut(s) 197, 432, 603
PspN4I GGNNCC 1 cut(s) 308
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 3 cut(s) 125, 371, 602
RsaNI GTAC 3 cut(s) 124, 370, 601
SatI GCNGC 5 cut(s) 400, 411, 414, 438, 666
Sau3AI GATC 1 cut(s) 186
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 3 cut(s) 199, 434, 605
SduI GDGCHC 3 cut(s) 309, 516, 614
SexAI ACCWGGT 1 cut(s) 603
SfaNI GCATC 2 cut(s) 163, 610
SfcI CTRYAG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SpeI ACTAGT 2 cut(s) 256, 536
Sse9I AATT 1 cut(s) 30
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 4 cut(s) 399, 491, 566, 666
SspMI CTAG 2 cut(s) 257, 537
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 3 cut(s) 197, 432, 603
TaaI ACNGT 2 cut(s) 444, 636
TaqI TCGA 1 cut(s) 44
TasI AATT 1 cut(s) 30
TatI WGTACW 2 cut(s) 123, 369
TauI GCSGC 2 cut(s) 402, 668
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 3 cut(s) 79, 272, 652
TseI GCWGC 3 cut(s) 410, 413, 437
Tsp45I GTSAC 3 cut(s) 79, 272, 652
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 510
XceI RCATGY 1 cut(s) 150
XspI CTAG 2 cut(s) 257, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.