MD10G1227000.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008334
Physical Location & Seq
Reverse (-)
32387543 .. 32390839
3297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1227000.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGAGGCCATTGGAAACGCAGGGACTGCAATTGGGATATTGTCGAAAGATGGAGTTGTGTTGGTAGGTGAAAAGAAGGTGACTTCCAAGCTCCTTCAGACCTCTACATCAACAGAGAAGATGTACAAGATTGATGACCACGTCGCATGTGCAGTGGCTGGGATAATGTCTGATGCCAACATCTTGATCAACACAGCCAGGGTCCAAGCACAGCGCTACACATTTGCTTACCAAGAGCCAATGCCTGTTGAACAACTAGTCCAATCTCTGTGTGACACCAAACAAGGCTACACGCAGTTTGGTGGGCTCCGTCCATTTGGCGTTTCGTTTCTGTTTGCAGGTTGGGACAAAAACTACGGTTTTCAGCTGTACATGAGCGACCCAAGTGGAAACTATGGCGGTTGGAAGGCTGCGGCCATTGGGGCAAACAACCAGGCAGCACAGTCAATGCTGAAGCAGGACTACAAGGATGAAATAACAAGAGAAGAGGCAGTTGAGCTTGCGCTGAAGGTGCTGAGCAAGACAATGGACAGCACTAGTCTGACATCCGATAAGCTCGAGTTGGCGGAAGTCTTTGTCTTGCCTTCGGGTAAGGTGAAGTACCAGGTGTGCTCCCCTGATGCACTGAGCAAACTGTTGGTGAAGAGTGGAGTGACTCAACCTGCCGCCGAGGCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

227

Amino Acids

24.37

Weight (kDa)

5.54

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 6 - 190 2.1e-60 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 329, 670
AciI CCGC 4 cut(s) 399, 413, 566, 666
AcoI YGGCCR 1 cut(s) 414
AcuI CTGAAG 3 cut(s) 80, 473, 527
AfaI GTAC 3 cut(s) 125, 371, 602
AfeI AGCGCT 1 cut(s) 215
AgsI TTSAA 1 cut(s) 251
AhlI ACTAGT 2 cut(s) 256, 536
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 3 cut(s) 197, 432, 603
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 4 cut(s) 91, 367, 499, 556
AluI AGCT 4 cut(s) 91, 367, 499, 556
Alw21I GWGCWC 1 cut(s) 614
AlwNI CAGNNNCTG 2 cut(s) 26, 158
Ama87I CYCGRG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 215
AoxI GGCC 3 cut(s) 6, 414, 672
ApeKI GCWGC 2 cut(s) 410, 437
AspLEI GCGC 2 cut(s) 216, 505
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 80, 91, 607, 652
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 446, 478
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 2 cut(s) 397, 449
BccI CCATC 1 cut(s) 44
BciT130I CCWGG 3 cut(s) 199, 434, 605
BclI TGATCA 1 cut(s) 186
BcuI ACTAGT 2 cut(s) 256, 536
BfaI CTAG 2 cut(s) 257, 537
BfoI RGCGCY 1 cut(s) 217
BfuAI ACCTGC 2 cut(s) 329, 670
BglI GCCNNNNNGGC 2 cut(s) 422, 671
BisI GCNGC 4 cut(s) 411, 414, 438, 666
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 4 cut(s) 412, 415, 439, 667
Bme1390I CCNGG 3 cut(s) 199, 434, 605
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 557
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 203, 308
BmrFI CCNGG 3 cut(s) 199, 434, 605
BmsI GCATC 2 cut(s) 163, 610
Bpu1102I GCTNAGC 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 198, 669
BseBI CCWGG 3 cut(s) 199, 434, 605
BseDI CCNNGG 2 cut(s) 198, 669
BseGI GGATG 2 cut(s) 475, 545
BseMII CTCAG 2 cut(s) 506, 617
BseXI GCAGC 2 cut(s) 397, 449
BseYI CCCAGC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 3 cut(s) 8, 416, 674
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 557
BslFI GGGAC 2 cut(s) 37, 359
BsmFI GGGAC 2 cut(s) 37, 359
BsnI GGCC 3 cut(s) 8, 416, 674
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 309, 614
Bsp1407I TGTACA 2 cut(s) 123, 369
Bsp143I GATC 1 cut(s) 186
Bsp1720I GCTNAGC 1 cut(s) 515
BspACI CCGC 4 cut(s) 399, 413, 566, 666
BspANI GGCC 3 cut(s) 8, 416, 674
BspCNI CTCAG 2 cut(s) 507, 618
BspLI GGNNCC 2 cut(s) 203, 308
BspMI ACCTGC 2 cut(s) 329, 670
BsrGI TGTACA 2 cut(s) 123, 369
BssECI CCNNGG 2 cut(s) 198, 669
BssMI GATC 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 199, 434, 605
Bst4CI ACNGT 3 cut(s) 359, 444, 636
Bst6I CTCTTC 2 cut(s) 480, 638
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 2 cut(s) 123, 369
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 3 cut(s) 515, 626, 678
BstF5I GGATG 2 cut(s) 475, 545
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 2 cut(s) 216, 505
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 4 cut(s) 26, 422, 511, 671
BstNI CCWGG 3 cut(s) 199, 434, 605
BstNSI RCATGY 1 cut(s) 150
BstSCI CCNGG 3 cut(s) 197, 432, 603
BstV1I GCAGC 2 cut(s) 397, 449
BsuRI GGCC 3 cut(s) 8, 416, 674
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 2 cut(s) 475, 545
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 159, 623
BveI ACCTGC 2 cut(s) 329, 670
Cac8I GCNNGC 1 cut(s) 501
CaiI CAGNNNCTG 2 cut(s) 26, 158
CfoI GCGC 2 cut(s) 216, 505
Cfr13I GGNCC 1 cut(s) 202
CsiI ACCWGGT 1 cut(s) 603
Csp6I GTAC 3 cut(s) 124, 370, 601
CviAII CATG 2 cut(s) 147, 373
CviQI GTAC 3 cut(s) 124, 370, 601
DdeI CTNAG 3 cut(s) 515, 626, 678
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 480, 638
EarI CTCTTC 2 cut(s) 480, 638
EciI GGCGGA 1 cut(s) 581
Eco147I AGGCCT 1 cut(s) 674
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 202
Eco47III AGCGCT 1 cut(s) 215
Eco57I CTGAAG 3 cut(s) 80, 473, 527
Eco88I CYCGRG 1 cut(s) 557
EcoRII CCWGG 3 cut(s) 197, 432, 603
EcoT38I GRGCYC 1 cut(s) 309
FaeI CATG 2 cut(s) 150, 376
FaiI YATR 3 cut(s) 148, 374, 396
FaqI GGGAC 2 cut(s) 37, 359
FatI CATG 2 cut(s) 146, 372
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 411, 414, 438, 666
FokI GGATG 2 cut(s) 482, 532
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 4 cut(s) 411, 414, 438, 666
FspBI CTAG 2 cut(s) 257, 537
GlaI GCGC 2 cut(s) 215, 504
GluI GCNGC 4 cut(s) 411, 414, 438, 666
GsaI CCCAGC 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 3 cut(s) 8, 416, 674
HhaI GCGC 2 cut(s) 216, 505
Hin1II CATG 2 cut(s) 150, 376
Hin6I GCGC 2 cut(s) 214, 503
HinP1I GCGC 2 cut(s) 214, 503
HinfI GANTC 1 cut(s) 655
HphI GGTGA 4 cut(s) 80, 91, 607, 652
Hpy188I TCNGA 4 cut(s) 99, 172, 543, 550
Hpy188III TCNNGA 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 5 cut(s) 70, 104, 400, 502, 594
HpyCH4III ACNGT 3 cut(s) 359, 444, 636
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 29, 152, 338, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 422, 511, 671
HpyF3I CTNAG 3 cut(s) 515, 626, 678
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 2 cut(s) 150, 376
HspAI GCGC 2 cut(s) 214, 503
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 96, 312, 617
Lsp1109I GCAGC 2 cut(s) 397, 449
LweI GCATC 2 cut(s) 163, 610
MabI ACCWGGT 1 cut(s) 603
MaeI CTAG 2 cut(s) 257, 537
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 79, 272, 652
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 3 cut(s) 130, 497, 655
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 309, 614
MluCI AATT 1 cut(s) 30
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 3 cut(s) 112, 481, 664
MspA1I CMGCKG 1 cut(s) 367
MspR9I CCNGG 3 cut(s) 199, 434, 605
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 3 cut(s) 199, 434, 605
MwoI GCNNNNNNNGC 4 cut(s) 26, 422, 511, 671
NdeII GATC 1 cut(s) 186
NlaIII CATG 2 cut(s) 150, 376
NlaIV GGNNCC 2 cut(s) 203, 308
NmuCI GTSAC 3 cut(s) 79, 272, 652
NspI RCATGY 1 cut(s) 150
PaeR7I CTCGAG 1 cut(s) 557
PceI AGGCCT 1 cut(s) 674
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 4 cut(s) 412, 415, 439, 667
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
Psp6I CCWGG 3 cut(s) 197, 432, 603
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 3 cut(s) 197, 432, 603
PspN4I GGNNCC 2 cut(s) 203, 308
PspPI GGNCC 1 cut(s) 202
PspXI VCTCGAGB 1 cut(s) 557
PstNI CAGNNNCTG 2 cut(s) 26, 158
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 3 cut(s) 125, 371, 602
RsaNI GTAC 3 cut(s) 124, 370, 601
SatI GCNGC 4 cut(s) 411, 414, 438, 666
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 3 cut(s) 199, 434, 605
SduI GDGCHC 2 cut(s) 309, 614
SexAI ACCWGGT 1 cut(s) 603
SfaNI GCATC 2 cut(s) 163, 610
Sfr274I CTCGAG 1 cut(s) 557
SinI GGWCC 1 cut(s) 202
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SpeI ACTAGT 2 cut(s) 256, 536
Sse9I AATT 1 cut(s) 30
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 4 cut(s) 399, 413, 566, 666
SspMI CTAG 2 cut(s) 257, 537
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 3 cut(s) 197, 432, 603
TaaI ACNGT 3 cut(s) 359, 444, 636
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 44, 558
TasI AATT 1 cut(s) 30
TatI WGTACW 2 cut(s) 123, 369
TauI GCSGC 2 cut(s) 416, 668
TscAI CASTG 2 cut(s) 159, 630
TseFI GTSAC 3 cut(s) 79, 272, 652
TseI GCWGC 2 cut(s) 410, 437
Tsp45I GTSAC 3 cut(s) 79, 272, 652
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 159, 630
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 202
XceI RCATGY 1 cut(s) 150
XhoI CTCGAG 1 cut(s) 557
XspI CTAG 2 cut(s) 257, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.