pycom17g12310

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008334
Physical Location & Seq
Forward (+)
10005113 .. 10005654
542 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g12310.1

Sequence Viewer

Length: 498 bp
ATGTACAAGATTGATGACCATCTTGCTTGTTCTGTGGCTGACATAATTTCTGATGCTCGGGTCCAAGCACAGCACTACATGTTTGCATACCGGGAACCCATGCCTGTGGAGCAGCTTGTTCAATCTCTTTGTGATACCAAGCAGGGGTGCACACAGTTTGGTGGTCTCTGGCCCTTTGGTGTTTCGTGTTTGTTTGCTGGTTGGGACAAGAATTATGGTTTCCAGCTTTACATGAGTGACCCTAGCGGAAATTATGCTGGCTGGAAAGCTGCGGTGATTGGGGCTAACAACCAAGCTGCGCAGTCGATAGTCGAGCTAGAGCTGAAGGTGCTCAGTAAAACCATGGACAGCACGAGTCTCACTCCGGAGAAGCTTGAGTTGGCTGAGGTATTCCTCCGCCTTCTGGAAAAAGTGAAATATCAAGTTTGTCCACCTGCATCTTTGAGTAACTTGTTGGAGAAGGTTGGGGTGAACCAACCAGCGACCGAGGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

166

Amino Acids

18.26

Weight (kDa)

4.85

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 2 - 108 3e-25 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
Acc16I TGCGCA 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 3 cut(s) 246, 272, 397
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 144, 403
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 7 cut(s) 115, 226, 269, 296, 316, 322, 373
AluI AGCT 7 cut(s) 115, 226, 269, 296, 316, 322, 373
Alw21I GWGCWC 2 cut(s) 152, 333
Alw26I GTCTC 2 cut(s) 170, 362
Alw44I GTGCAC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 1 cut(s) 170
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 3 cut(s) 112, 269, 296
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 61, 171
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 286, 481
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 152
BauI CACGAG 1 cut(s) 352
Bbv12I GWGCWC 2 cut(s) 152, 333
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 3 cut(s) 124, 256, 283
BccI CCATC 1 cut(s) 27
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 170, 362
BfaI CTAG 2 cut(s) 243, 317
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 3 cut(s) 113, 270, 297
BlsI GCNGC 3 cut(s) 114, 271, 298
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 61, 171
BmiI GGNNCC 2 cut(s) 62, 96
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 2 cut(s) 43, 446
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 342, 486
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 403
Bse8I GATNNNNATC 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 342, 486
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 2 cut(s) 144, 403
BseMII CTCAG 2 cut(s) 346, 375
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 3 cut(s) 124, 256, 283
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 152, 333
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 2 cut(s) 91, 365
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 144, 403
BsmAI GTCTC 2 cut(s) 170, 362
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 152, 333
Bsp13I TCCGGA 1 cut(s) 364
Bsp1407I TGTACA 1 cut(s) 3
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 3 cut(s) 246, 272, 397
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 2 cut(s) 345, 376
BspEI TCCGGA 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 62, 96
BspMI ACCTGC 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 2 cut(s) 342, 486
BssSI CACGAG 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 332, 384
BstDSI CCRYGG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 301
BstMAI GTCTC 2 cut(s) 170, 362
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 2 cut(s) 109, 328
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 152
BstV1I GCAGC 3 cut(s) 124, 256, 283
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 1 cut(s) 172
BtgI CCRYGG 1 cut(s) 342
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 2 cut(s) 61, 171
Csp6I GTAC 1 cut(s) 4
CviAII CATG 4 cut(s) 79, 100, 232, 343
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 332, 384
EciI GGCGGA 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 4 cut(s) 82, 103, 235, 346
FaiI YATR 8 cut(s) 44, 80, 88, 101, 216, 233, 255, 344
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 78, 99, 231, 342
Fnu4HI GCNGC 3 cut(s) 113, 270, 297
Fsp4HI GCNGC 3 cut(s) 113, 270, 297
FspBI CTAG 2 cut(s) 243, 317
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 1 cut(s) 300
GluI GCNGC 3 cut(s) 113, 270, 297
HaeIII GGCC 1 cut(s) 172
HapII CCGG 2 cut(s) 91, 365
HhaI GCGC 1 cut(s) 301
Hin1II CATG 4 cut(s) 82, 103, 235, 346
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 2 cut(s) 355, 491
HpaII CCGG 2 cut(s) 91, 365
HphI GGTGA 2 cut(s) 286, 481
Hpy166II GTNNAC 3 cut(s) 150, 431, 472
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 2 cut(s) 365, 404
Hpy8I GTNNAC 3 cut(s) 150, 431, 472
HpyAV CCTTC 3 cut(s) 319, 410, 454
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 86, 150, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 328
HpyF3I CTNAG 2 cut(s) 332, 384
Hsp92II CATG 4 cut(s) 82, 103, 235, 346
HspAI GCGC 1 cut(s) 299
Kpn2I TCCGGA 1 cut(s) 364
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 3 cut(s) 124, 256, 283
LweI GCATC 2 cut(s) 43, 446
MaeI CTAG 2 cut(s) 243, 317
MaeIII GTNAC 2 cut(s) 236, 446
MhlI GDGCHC 2 cut(s) 152, 333
MluCI AATT 3 cut(s) 45, 211, 250
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 3 cut(s) 379, 404, 481
MroI TCCGGA 1 cut(s) 364
MseI TTAA 1 cut(s) 496
MslI CAYNNNNRTG 1 cut(s) 104
MspI CCGG 2 cut(s) 91, 365
MspR9I CCNGG 1 cut(s) 92
MwoI GCNNNNNNNGC 2 cut(s) 109, 328
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 342
NlaIII CATG 4 cut(s) 82, 103, 235, 346
NlaIV GGNNCC 2 cut(s) 62, 96
NmuCI GTSAC 1 cut(s) 236
NsbI TGCGCA 1 cut(s) 300
NspI RCATGY 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 442
PciI ACATGT 1 cut(s) 78
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 3 cut(s) 114, 271, 298
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PscI ACATGT 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 62, 96
PspPI GGNCC 2 cut(s) 61, 171
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 3 cut(s) 113, 270, 297
Sau96I GGNCC 2 cut(s) 61, 171
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 152, 333
SfaNI GCATC 2 cut(s) 43, 446
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 3 cut(s) 45, 211, 250
SsiI CCGC 3 cut(s) 246, 272, 397
SspMI CTAG 2 cut(s) 243, 317
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 305, 312
TasI AATT 3 cut(s) 45, 211, 250
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 3 cut(s) 112, 269, 296
Tsp45I GTSAC 1 cut(s) 236
VneI GTGCAC 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 61
XceI RCATGY 1 cut(s) 82
XspI CTAG 2 cut(s) 243, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.